CN205786665U - A kind of test strips of quick detection Muscovy duck parvovirus antibody - Google Patents

Abstract

A kind of test strips of quick detection Muscovy duck parvovirus antibody, including supporting layer, sample adsorption fibrous layer is pasted in supporting layer one end, the other end pastes water accepting layer, bonding partially cellulose membrane layer between adsorbing fiber layer and the water accepting layer of supporting layer and gold mark fibrous layer, adsorbing fiber layer, the gold intersection fiber that splices each other between fibrous layer, cellulose membrane layer, water accepting layer of mark cross one another infiltration.This utility model at least includes following beneficial effect: (1) detection high specificity, sensitivity is high.The test strips that this utility model provides is prepared from based on the restructuring Muscovy duck parvovirus VP2 albumen of gold colloidal mark Muscovy duck parvovirus or gold labelling, gold secondary disease poison is formed without covalent bond between gold grain and virus protein molecule, the two is combined by the Van der Waals force between the charges of different polarity, gold colloidal mark is the least on specificity and the affinity impact of virus protein, and has higher trace rate.(2) easy and simple to handle, quickly.

Description

A kind of test strips of quick detection Muscovy duck parvovirus antibody

Technical field

This utility model relates to a kind of utensil detecting Muscovy duck parvovirus antibody, particularly relates to a kind of quickly detection kind The test strips of Duck parvovirus antibody.

Background technology

Muscovy duck parvovirus is sick (Muscovy duck parvovirus Disease, MDPD), is by the tiny disease of Muscovy duck Poison (Muscovy duck parvovirus, MDPV) causes, with the one suffered from diarrhoea, breathe and weak foot is main clinical symptom Acute, septic infectious disease, this disease takes place mostly in the Muscovy duck within 3 week old, therefore sick also known as three weeks, and its main clinic symptoms is Dyspnea, anorexia, suffer from diarrhoea, be dehydrated and become thin rapidly.Owing to this disease is propagated fast, sickness rate, mortality rate are the highest, support to Muscovy duck Grow and cause Important Economic to lose.

Muscovy duck parvovirus disease comes across China Fujian early than the middle and late stage eighties in 20th century and France is western Brittany area, the most widely distributed.Owing to MDPV belongs to parvovirus, strong to environmental resistance, 65 DEG C of heating 30min are to it Titre is without impact, insensitive to organic solvents such as ether, stable to pancreatin and pH3, it is difficult to eliminate from environment, significantly impact The sound development of China's duck culturing industry.

Quickly detection MDPDV antibody, is possible not only to evaluate immune effect of vaccine, and can diagnose with adjuvant clinical, be to have Effect controls the premise of MDPD.The method of detection MDPDV antibody mainly has agar immunodiffusion test (AGP), serum to neutralize at present The methods such as experiment, IFA and ELISA.But, AGP is the longest, answers with the operation of the detection technique such as experiment, IFA and ELISA in serum Miscellaneous, instrument and equipment, process that needs are expensive are longer, time and effort consuming, need particular instrument equipment and professional and technical personnel's operation, very Difficult popularize in basic unit, it is impossible to meet production line or epidemic disease on-the-spot quickly detect needs.It would therefore be highly desirable to there is a kind of quickly detection The equipment of fowl Muscovy duck parvovirus antibody and technology.

Utility model content

A purpose of the present utility model is to provide the test strips of a kind of quick detection Muscovy duck parvovirus antibody, and provides The advantage that at least will be described later.

This utility model it is also an object that provide a kind of result intuitive display, accurately, quick detection MDPV antibody Test strips, compared with other detection methods, this test strips high specificity, highly sensitive, easy and simple to handle, result show quickly, inspection Survey with low cost.

The technical solution of the utility model particularly as follows:

The test strips of a kind of quick detection Muscovy duck parvovirus antibody, including supporting layer, supporting layer one end is pasted sample and is inhaled Attached fibrous layer, the other end pastes water accepting layer, the bonding partially cellulose membrane between adsorbing fiber layer and the water accepting layer of supporting layer Layer and gold mark fibrous layer, the intersection that adsorbing fiber layer, gold mark splice between fibrous layer, cellulose membrane layer, water accepting layer each other is fine Dimension crosses one another infiltration, it is characterised in that: described cellulose membrane layer is provided with labelling, and to comprise the Muscovy duck parvovirus with purification anti- The detection trace and comprise the trace that compares with sheep or rabbit anti-Muscovy duck parvovirus antibody of body, detection trace is with to compare trace arranged side by side Being arranged on cellulose membrane layer, that detects trace is located proximate to adsorbing fiber layer；Gold mark fibrous layer is for being attached with colloid gold label Muscovy duck parvovirus or colloid gold label restructuring Muscovy duck parvovirus VP2 albumen glass cotton make；This test strips is provided with The fibrolaminar one end of sample adsorption is water intake end, and the one end being provided with water accepting layer is holding end, and water intake end outer side covers has white First protecting film, holding end is coated with the second protecting film；At the protecting film that adsorbing fiber layer is corresponding with gold mark fibrous layer intersection On be partial at sample adsorption fibrous layer side 0.5cm be printed on trace line；Holding end is provided with the groove being easy to grip.

Supporting layer is set to latticed at water intake end, generally circular in shape, square, triangle, the rhombus of its mesh, six limits Shape.

Supporting layer is for make with plastic slice bar.

This utility model at least includes following beneficial effect:

(1) detection high specificity, sensitivity is high.The test strips that this utility model provides is with gold colloidal mark Muscovy duck parvovirus Or be prepared from based on the restructuring Muscovy duck parvovirus VP2 albumen of gold labelling, in gold secondary disease poison, gold grain divides with virus protein Being formed without covalent bond between son, the two is combined by the Van der Waals force between the charges of different polarity, the gold colloidal mark spy to virus protein The opposite sex and affinity impact are the least, and have higher trace rate.

(2) easy and simple to handle, quickly.Test strips of the present utility model is in use without additional any Other Instruments and examination Agent, as long as by its test lead insertion measuring samples liquid about 30 seconds, i.e. can determine that testing result in about 5 minutes.

(3) result intuitive display, accurately.Test strips of the present utility model is to show henna detection trace and comparison print Mark, as the positive detected and negative trace, i.e. shows two brownish red traces on cellulose membrane, represents at detected sample In have antiviral antibody to detect, result is positive；On cellulose membrane, only one brownish red of display compares trace C, represents tested Surveying in sample liquid and do not detect antiviral antibody, result is negative.Result judges directly perceived, accurate, simple and clear, is difficult to false negative occur Judge by accident with false positive.

(4) investment and testing cost are reduced.Test strips of the present utility model in use, is not required to separately join Other Instruments, set Standby and reagent, saves big measuring appratus, equipment and additive reagent expense；Specialty and layman all can carry out scene whenever and wherever possible Detection, it is not necessary to payment expert diagnosis Laboratory Fee or feeding sample remove the travelling expenses of diagnosis room, saves testing cost, and testing cost is low.

(5) applied range, it is simple to popularity is applied.The test strips that this utility model provides is simple to operate, becomes " a step Formula " or " foolproof ", and be convenient for carrying and preserve, the needs of different levels personnel can be met, including specialty chemical examination, customs's inspection Epidemic disease, health and epidemic prevention, quality-monitoring, livestock products processing, intensive culture, to individuality cultivation etc., have wide market prospect and relatively Good economical, societal benefits.

(6) this utility model is according to the ultimate principle of ELISA, detects antiviral antibody with immune chromatography test paper, utilizes micropore The diafiltration concentration of filter membrane and capillarity, forwarded to antigen-antibody reaction on solid phase filter membrane by the Traditional liquid phase environment of ELISA Quickly carry out, and use gold colloidal trace to replace enzyme trace, with the colour developing situation of direct visual perception gold colloidal, immediately obtain inspection Surveying result, therefore the method is easier than serological methods such as ELISA, quickly.

Accompanying drawing explanation

Fig. 1 is generalized section of the present utility model；

Fig. 2 is schematic top plan view of the present utility model；

Fig. 3 is the schematic diagram of supporting layer.

Detailed description of the invention

Below in conjunction with the accompanying drawings this utility model is described in further detail, to make those skilled in the art with reference to explanation Book word can be implemented according to this.

Such as Fig. 1 and Fig. 2, the test strips of a kind of quick detection Muscovy duck parvovirus antibody, including supporting layer 1, supporting layer 1 one End pastes sample adsorption fibrous layer 2, and the other end pastes water accepting layer 5, between the adsorbing fiber layer 2 and water accepting layer 5 of supporting layer 1 Bonding partially cellulose membrane layer 4 and gold mark fibrous layer 3, adsorbing fiber layer 2, gold mark fibrous layer 3, cellulose membrane layer 4, water accepting layer 5 Between the intersection fiber that splices each other cross one another infiltration, cellulose membrane layer 4 is provided with labelling and comprises the Muscovy duck with purification The detection trace 6 of parvovirus antibody compares trace 7 with comprising with sheep or rabbit anti-Muscovy duck parvovirus antibody, detects trace 6 With compare trace 7 arranged in parallel on cellulose membrane layer, detection trace 6 be located proximate to adsorbing fiber layer, the generation of detection trace 6 Number it is C；The code name of comparison trace 7 is T.

Gold mark fibrous layer 3 is that to be attached with the restructuring Muscovy duck of the Muscovy duck parvovirus of colloid gold label or colloid gold label tiny The glass cotton of virus VP 2 albumen is made.

It is water intake end (also referred to as test lead) that this test strips is provided with one end of sample adsorption fibrous layer 2, is provided with water accepting layer 5 One end is holding end, and water intake end outer side covers has the first protecting film 81 of white, and holding end is coated with other color such as yellow The second protecting film 82.Sample adsorption is partial to by the protecting film that adsorbing fiber layer 2 is corresponding with gold mark fibrous layer 3 intersection fine Being printed on trace line 9 at dimension layer 2 side 0.5cm, the right-hand member of trace line is printed on arrow and MAX printed words.

Even if it is true that be provided with the protecting film of different colours, it is also possible to make testing staff be mixed with holding end by water intake end Confuse, cause detection accident.So, the groove 83 or projection (only having drawn groove in figure) being easy to grip it is provided with at holding end, recessed Groove 83 or raised marking have thumb shape, so arrange, and testing staff can be facilitated to distinguish water end (W.E.) and holding end, be also convenient for Grip, more meet ergonomics.

If groove 83, groove 83 extends inward into water accepting layer 5 or supporting layer 1, convenient gripping.

If projection, projection can be elastomeric material, convenient gripping.

Supporting layer 1 is for make with plastic slice bar.

Such as Fig. 3, supporting layer 1 is set to latticed at water intake end, generally circular in shape, square, the triangle of its mesh 11, Pedicellus et Pericarpium Trapae Shape, hexagon.During normal ELISA test strip, the opposite side of water accepting layer 5 only supporting layer 1 can absorb water (solution), and this reality It is latticed with the water intake end of novel supporting layer 1, water accepting layer 5 can be made also to be inhaled by mesh 11 in the side of supporting layer 1 Water, so since, water accepting layer 5 can absorb water with two sides, accelerates the speed of water suction, shortens the time used by detection, improves Work efficiency.

Sample adsorption fibrous layer 2 is for make with glass cotton.

Cellulose membrane layer 4 is make with nitrocellulose filter.

Water accepting layer 5 is for be made up of absorbent filter.

Detection operational approach of the present utility model

The preparation of a detection sample liquid: the aseptic blood taking tested animal, and separate serum, make 1:200 with normal saline Dilute serum again, obtains testing sample；

B detects operation: inserted by this test strips water intake end in detected sample, and insertion depth is less than trace line, and about 30 Test strips, horizontal positioned about 1-5 minute, observed result simultaneously is taken out after Miao.

C result judges: if only demonstrating a brownish red comparison trace C on the cellulose membrane of test strips, representing and surveying Inspection result is negative, and illustrates not detect Muscovy duck parvovirus antibody in test sample liquid；If the cellulose in test strips There is henna comparison trace C and detection trace T in film, represents that testing result is positive, and i.e. detects Muscovy duck in measuring samples Parvovirus antibody；If not having any brownish red trace to show on cellulose membrane, then show that test strips had lost efficacy or operated By mistake.

Cleaning Principle of the present utility model:

After the i.e. adsorbing fiber layer of test strips test lead inserts detected sample solution, solution to be checked is by chromatography zone of action Gold mark antigen in dynamic sick Muscovy duck parvovirus antibody to be checked and gold mark fibrous membrane spreads to cellulose membrane together, and eventually penetrates In the water accepting layer of handle end, the gold mark Muscovy duck parvovirus that in diffusion process, antiviral antibody to be checked can be corresponding with this antiviral antibody Or gold indicated weight group Muscovy duck parvovirus VP2 albumen combines, and then detect the virus combination in trace on cellulose membrane, thus Demonstrate henna detection trace T；Compare the sheep in trace or rabbit anti-Muscovy duck parvovirus IgG then can be with gold mark Muscovy duck Parvovirus or gold indicated weight group Muscovy duck parvovirus VP2 protein binding, form brownish red comparison trace C.If measuring samples liquid In there is no MDPV antibody, test strips only demonstrates brownish red comparison trace C；If there is no any brownish red on cellulose membrane Trace shows, then show that test strips had lost efficacy or operational error.

Claims (1)

1. a test strips for quick detection Muscovy duck parvovirus antibody, including supporting layer (1), sample is pasted in supporting layer (1) one end Product adsorbing fiber layer (2), the other end pastes water accepting layer (5), between the adsorbing fiber layer (2) and water accepting layer (5) of supporting layer (1) Bonding partially cellulose membrane layer (4) with gold mark fibrous layer (3), adsorbing fiber layer (2), gold mark fibrous layer (3), cellulose membrane layer (4), the intersection fiber spliced each other between water accepting layer (5) crosses one another infiltration, it is characterised in that:

Described cellulose membrane layer (4) be provided be marked with the detection trace (6) that comprises the Muscovy duck parvovirus antibody with purification with Comprise with the comparison trace (7) of sheep or rabbit anti-Muscovy duck parvovirus antibody, detect trace (6) and compare trace (7) arranged in parallel On cellulose membrane layer, that detects trace (6) is located proximate to adsorbing fiber layer；

Gold mark fibrous layer (3) is for be attached with the Muscovy duck parvovirus of colloid gold label or the tiny disease of restructuring Muscovy duck of colloid gold label The glass cotton of poison VP2 albumen is made；

It is water intake end that this test strips is provided with one end of sample adsorption fibrous layer (2), and being provided with water accepting layer (5) one end is holding end, enters Water end (W.E.) outer side covers has first protecting film (81) of white, and holding end is coated with the second protecting film (82)；In adsorbing fiber layer (2) It is partial at sample adsorption fibrous layer (2) side 0.5cm on the protecting film corresponding with gold mark fibrous layer (3) intersection be printed on trace Line (9)；

Holding end is provided with the groove (83) being easy to grip；

Supporting layer (1) is set to latticed at water intake end, its mesh (11) be shaped as square；

Described supporting layer (1) is for make with plastic slice bar；

Water accepting layer (5) is for be made up of absorbent filter.