CN219142858U

CN219142858U - Detection card for rapidly detecting influenza A virus in saliva

Info

Publication number: CN219142858U
Application number: CN202222450477.2U
Authority: CN
Inventors: 刘国迪; 顾章杰; 田晓丽
Original assignee: Shanghai Yihao Biotechnology Co ltd
Current assignee: Shanghai Yihao Biotechnology Co ltd
Priority date: 2022-09-16
Filing date: 2022-09-16
Publication date: 2023-06-06
Anticipated expiration: 2032-09-16

Abstract

The utility model provides a detection card for rapidly detecting influenza A virus in saliva, which relates to the technical field of infectious virus detection, and comprises a saliva liquid suction head, a sample pad, a colloidal gold pad, a nitrocellulose reaction membrane, a water absorption pad and a plastic card.

Description

Detection card for rapidly detecting influenza A virus in saliva

Technical Field

The utility model relates to the technical field of biological detection, in particular to a detection card for rapidly detecting influenza A virus in saliva.

Background

Human influenza is mainly caused by influenza a virus and influenza b virus. In particular, influenza a virus has a very high frequency of antigenic variation and is rapidly transmitted mainly by droplets in the air, contact between susceptible and infected persons, or contact with contaminated articles, and is the main cause of recurrent pandemic of stage influenza. Typical clinical symptoms of influenza a are acute hyperthermia, general pain, significant debilitation and respiratory symptoms, severe ones can cause pneumonia and even be life threatening. The common real-time quantitative fluorescent PCR nucleic acid detection reagent for influenza A virus is limited by complicated operation, long time consumption, cost and the like, and cannot be popularized and applied on a large scale, and the common rapid detection means generally aim at swab samples, the swab samples need training, are relatively difficult to operate, and result errors are easily caused by improper sampling. Therefore, the development of the rapid detection method for the influenza A virus by taking saliva as a sample has important significance for timely blocking the pandemic of the influenza virus and maintaining the life health of vast people.

Disclosure of Invention

The utility model provides a detection card for rapidly detecting influenza A virus in saliva to solve the problems.

In order to solve the technical problems, the technical scheme of the utility model is as follows: a detection card for rapidly detecting influenza A virus in saliva comprises a saliva liquid suction head, a sample pad, a colloidal gold pad, a nitrocellulose reaction membrane, a water absorption pad and a plastic card;

the saliva imbibition head, the sample pad, the colloidal gold pad, the nitrocellulose reaction membrane and the water absorption pad are sequentially stuck on the plastic card, and the colloidal gold pad contains a colloidal gold marked influenza A antibody I.

Further, the nitrocellulose reaction membrane is provided with a detection line and a quality control line, the detection line is positioned on one side of the nitrocellulose reaction membrane, which is close to the colloidal gold pad, the detection line is coated with an influenza A virus antibody II, and the quality control line is positioned on one side of the nitrocellulose reaction membrane, which is far away from the colloidal gold pad, and contains a goat anti-mouse IgG polyclonal antibody.

Further, the influenza A virus antibody I and the influenza A virus antibody II are antibodies with different epitopes, and the antibodies are prepared manually.

Further, saliva is taken as a detection sample by the detection card.

The utility model has the following beneficial effects: the detection card for rapidly detecting the influenza A virus in saliva can specifically identify the influenza A virus, saliva is used as a detection sample, the sample is easy to obtain, a sampling swab is not needed, the influenza A virus detection flow can be simplified, the operation is simple and convenient, the detection period is short, and the judgment is easy.

Drawings

FIG. 1 is a front view of a test card for rapid detection of influenza A virus in saliva according to the present utility model;

FIG. 2 is a positive schematic diagram of the detection result of a detection card for rapidly detecting influenza A virus in saliva;

FIG. 3 is a negative schematic diagram of the detection result of the detection card for rapidly detecting influenza A virus in saliva;

fig. 4 (1) and 4 (2) are schematic diagrams showing that the detection result of the detection card for rapidly detecting influenza a virus in saliva is invalid.

As shown in the figure: 1. a saliva suction head; 2. a sample pad; 3. a colloidal gold pad; 4. a nitrocellulose reaction membrane; 5. a water absorbing pad; 6. a plastic card; t, detecting lines; C. and a quality control line.

Detailed Description

The utility model is further illustrated by the following specific examples, which are intended to illustrate the problem and to explain the utility model, without limiting it.

Example 1

A detection card for rapidly detecting influenza A virus in saliva is shown in figure 1, and comprises a saliva liquid suction head 1, a sample pad 2, a colloidal gold pad 3, a nitrocellulose reaction membrane 4, a water absorption pad 5 and a plastic card 6; in this embodiment, the saliva suction head 1, the sample pad 2, the colloidal gold pad 3, the nitrocellulose reaction membrane 4 and the water absorption pad 5 are sequentially adhered to the plastic card 6, and the colloidal gold pad is a colloidal gold pad containing a colloidal gold-labeled influenza a antibody one; in this embodiment, the nitrocellulose reaction membrane is provided with a detection line T and a quality control line C, the detection line T is located at one side of the nitrocellulose reaction membrane 4 close to the colloidal gold pad 3, the detection line T is coated with influenza a virus antibodies two, and the quality control line C is located at one side of the nitrocellulose reaction membrane 4 away from the colloidal gold pad 3 and contains goat anti-mouse IgG polyclonal antibodies; in this embodiment, the influenza a virus antibody a and the influenza a virus antibody b are antibodies with different epitopes, and the antibodies are all prepared manually.

The application mode of the utility model is as follows:

1) Collecting a sufficient amount of saliva sample;

2) The saliva suction head 1 is contacted with the saliva sample for at least 2 minutes, wherein the saliva suction head 1 of the detection card can be immersed into the saliva sample, and the saliva sample moves towards the water absorption pad 5 along each attachment on the plastic card 6 in sequence;

3) When liquid appears in the observation window, the liquid is placed on a clean and flat workbench, and the detection result of the influenza A virus in the saliva sample is obtained after 15 minutes.

If the saliva sample contains influenza a virus, the saliva sample specifically binds with the colloidal gold labeled influenza a virus antibody on the detection card to form a complex, and then proceeds, and in the process of passing through the nitrocellulose membrane 4, the saliva sample specifically binds with the influenza a virus antibody 2, a red color appears at the detection line T, which indicates that the sample to be detected is positive, and refer to fig. 2.

If the saliva product does not contain influenza A virus, the detection line T does not appear red, and the sample is negative; and the colloidal gold labeled influenza A virus antibody 1 continues to advance to be combined with the goat anti-mouse IgG polyclonal antibody, and the red color appears at the C band of the quality control line, referring to FIG. 3;

whether the sample contains influenza A virus or not, the colloidal gold labeled influenza A virus antibody 1 can continuously move to the quality control line C and combine with the goat anti-mouse IgG polyclonal antibody coated on the quality control line C to form a red strip, namely the quality control line. If the quality control line does not appear in the detection process, the failure or misoperation of the detection card is proved, the result is invalid, and the detection needs to be re-performed, and the two results shown as 4 (1) and 4 (2) in fig. 4 are referred to.

The foregoing is merely a preferred embodiment of the utility model, and it should be noted that modifications could be made by those skilled in the art without departing from the principles of the utility model, which modifications would also be considered to be within the scope of the utility model.

Claims

1. A detection card for rapidly detecting influenza a virus in saliva, which is characterized in that: comprises a saliva suction head (1), a sample pad (2), a colloidal gold pad (3), a nitrocellulose reaction membrane (4), a water absorption pad (5) and a plastic card (6);

the saliva pipette head (1), the sample pad (2), the colloidal gold pad (3), the nitrocellulose reaction membrane (4) and the water absorption pad (5) are sequentially stuck to the plastic card (6), and the colloidal gold pad (3) is provided with a colloidal gold marked influenza A antibody I.

2. A test card for rapid detection of influenza a virus in saliva as defined in claim 1, wherein: the detection line (T) is positioned on one side of the nitrocellulose reaction membrane (4) close to the colloidal gold pad (3), the detection line (T) is coated with influenza A virus antibodies II, and the quality control line (C) is positioned on one side of the nitrocellulose reaction membrane (4) far away from the colloidal gold pad (3) and contains goat anti-mouse IgG polyclonal antibodies.

3. A test card for rapid detection of influenza a virus in saliva as defined in claim 2, wherein: the influenza A virus antibody I and the influenza A virus antibody II are antibodies with different epitopes, and the antibodies are prepared by manpower.

4. A test card for rapid detection of influenza a virus in saliva as defined in claim 1, wherein: the detection card takes saliva as a detection sample.