CN210347665U - African swine fever virus micro-fluidic detector - Google Patents
African swine fever virus micro-fluidic detector Download PDFInfo
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- CN210347665U CN210347665U CN201920617118.7U CN201920617118U CN210347665U CN 210347665 U CN210347665 U CN 210347665U CN 201920617118 U CN201920617118 U CN 201920617118U CN 210347665 U CN210347665 U CN 210347665U
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Abstract
The utility model relates to the technical field of immunodetection, and discloses an African swine fever virus microfluidic detector, which comprises a microfluidic chip, a temperature control device and a detection device, wherein the temperature control device and the detection device are respectively connected with the microfluidic chip, and the microfluidic chip comprises a sample inlet area, a sample processing area, a detection area and a sample outlet area which are sequentially communicated; the sample introduction area is provided with a quantitative ring and is communicated with the sample treatment area through the quantitative ring; the detection area comprises a positive control area, a blank control area and a reaction area, biochemical reagents for detecting undetermined indexes are coated in the positive control area, the blank control area and the reaction area, and an emptying valve is arranged on the sample outlet area; the micro-fluidic chip also comprises a buffer solution pool and a reagent pool, wherein the buffer solution pool and the reagent pool are respectively communicated with the sample processing area. The utility model discloses can detect the African swine fever virus at the scene, and detect fast, convenient operation, can be used for quantitative analysis.
Description
Technical Field
The utility model relates to an immunodetection technical field especially relates to a little fluidic detector of African swine fever virus.
Background
African swine fever is an acute, hemorrhagic and virulent infectious disease caused by African Swine Fever Virus (ASFV) infecting domestic pigs and various wild pigs (such as African wild pigs, European wild pigs, etc.). The acute cough-relieving and acute infection has the characteristics of short disease process, high mortality rate of the most acute and acute infections up to 100%, fever (up to 40-42 ℃) as clinical manifestations, accelerated heartbeat, dyspnea, partial cough, serous or mucoid purulent secretion of eyes and nose, cyanosis of skin and obvious bleeding of lymph nodes, kidney and gastrointestinal mucosa.
China is a big country for raising pigs and consuming pork, the marketing amount, the stocking amount and the pork consumption amount of live pigs are all in the top of the world, the total import amount of the pigs and pork products is huge every year, and the trade is frequent in a plurality of countries; moreover, China has frequent trips with passengers in other countries, and the number of commodities carried by the passengers is large and the types of the commodities are various. Therefore, the risk of transferring the African swine fever into China is increased, and once the African swine fever is transferred, the direct and indirect loss caused by the African swine fever is immeasurable.
The key to the prevention and control of viral animal epidemic diseases is the rapid detection of epidemic viruses. The existing detection method of African swine fever virus mainly comprises a virus separation method, a reverse transcription polymerase chain reaction method (RT-PCR) and a colloidal gold immunochromatography test strip. The virus separation method is a gold standard method for diagnosis and identification at present, has high sensitivity, but has long detection time which needs 7-10 days; RT-PCR is a detection method recommended by the world health organization, needs 3-6 hours, has high sensitivity, and needs complex sample pretreatment; the test strip has short detection time, usually within 15 minutes, but has low sensitivity and high false positive rate. The existing African swine fever virus detection method has the defects of overlong time, complex operation or low accuracy, and limits the primary animal epidemic disease prevention and control mechanism to carry out the rapid screening work of animal epidemic diseases to a certain extent.
At present, the invention patent with the patent application number of 201410334079.1 discloses a virus detector, which comprises an interdigital array microelectrode, a microfluidic detection cell and an impedance detection module; when a sample containing viruses is injected into the microfluidic detection cell, antibodies or other biological recognition materials fixed on the surface of the interdigital array microelectrode embedded in the microfluidic detection cell are combined with the viruses to generate impedance change, impedance measurement is carried out through an impedance detection module, data processing is carried out by using a standard curve, and the content of the avian influenza viruses can be rapidly and quantitatively detected. The detector has the advantages of high detection speed, convenient operation, quantitative analysis and field detection, but the micro-fluidic detector specially used for detecting and analyzing the African swine fever virus does not exist in the current market.
SUMMERY OF THE UTILITY MODEL
The utility model provides an African swine fever virus micro-fluidic detector to the above-mentioned shortcoming that exists among the prior art.
In order to solve the technical problem, the utility model discloses a following technical scheme can solve:
the African swine fever virus micro-fluidic detector comprises a micro-fluidic chip, a temperature control device and a detection device, wherein the temperature control device and the detection device are respectively connected with the micro-fluidic chip, and the micro-fluidic chip comprises a sample inlet area, a sample processing area, a detection area and a sample outlet area which are sequentially communicated; the sample introduction area is provided with a quantitative ring and is communicated with the sample treatment area through the quantitative ring; the detection area comprises a positive control area, a blank control area and a reaction area, biochemical reagents for detecting undetermined indexes are coated in the positive control area, the blank control area and the reaction area, the sample processing area is respectively communicated with the positive control area, the blank control area and the reaction area, and an emptying valve is arranged on the sample outlet area; the micro-fluidic chip also comprises a buffer solution pool, a reagent pool and a dosing pool, wherein a buffer solution for processing a sample is arranged in the buffer solution pool, a reagent for processing the sample is arranged in the reagent pool, the buffer solution pool and the reagent pool are respectively communicated with the sample processing area, and the dosing pool is communicated with the detection area; the sample processing area and the detection area are respectively connected with a temperature control device, and the detection device is connected with the detection area.
Preferably, the temperature control device comprises a heating device and a temperature sensor, the heating device is connected with the sample processing area and the detection area, the heating device is used for heating the solution in the sample processing area and the detection area, and the temperature sensor is used for detecting the temperature of the solution in the sample processing area and the detection area.
Preferably, the detection device is a microplate reader, and the microplate reader is connected with the detection area and is used for detecting signals of the solution in the detection area.
Preferably, the detection zone further comprises a negative control zone within which a biochemical reagent for detecting the indicia to be determined is coated, the negative control zone being in communication with the sample treatment zone.
Preferably, the number of the sample injection zones is 3, the number of the sample outlet zones is 1, the number of the positive control zone, the number of the negative control zone and the blank control zone in the detection zone are respectively 1, the number of the reaction zones is at least 2, one sample injection zone is respectively used for the positive control zone and the negative control zone, and the sample injection zone and the sample outlet zone are shared by the blank control zone and the reaction zones.
Preferably, the positive control area, the blank control area, the reaction area and the negative control area have a length of 8.5-10 mm and a width of 5-6 mm. In this case, less biochemical reagents can be required, which reduces the detection cost and also contributes to more complete reaction.
The sample is quantitatively conveyed to a sample treatment area through a quantitative ring for corresponding treatment, the treated sample respectively enters a positive control area, a blank control area, a negative control area and a reaction area, and is subjected to reaction with a biochemical reagent which is coated in the detection area and is used for detecting undetermined indexes, and then the detection is carried out by using a detection device. The temperature control device can provide proper reaction temperature for sample treatment and reaction processes, so that the experiment can be completed better.
The utility model discloses owing to adopted above technical scheme, have apparent technological effect:
the micro-fluidic detector of the utility model can detect the African swine fever virus, can detect the African swine fever virus on site, has fast detection speed and convenient operation, and can be used for quantitative analysis; meanwhile, the kit is provided with a positive control area, a blank control area, a negative control area and a plurality of reaction areas, a plurality of indexes can be tested at one time, corresponding control tests can be completed, biochemical reagents for detecting the indexes to be determined are coated in the detection areas, the detection is convenient and sensitive, and unnecessary experimental errors can be reduced; the reagent pool and the buffer pool are communicated with the sample treatment area, so that the liquid can be taken quickly, and the sample can be conveniently treated.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural diagram of the microfluidic chip.
Fig. 3 is a schematic structural view of the microfluidic chip of example 2.
The names of the parts indicated by the numerical references in the drawings are as follows: 1-microfluidic chip, 11-sample introduction zone, 12-sample treatment zone, 13-detection zone, 131-positive control zone, 132-negative control zone, 133-blank control zone, 134-reaction zone, 14-sample discharge zone, 15-quantification ring, 16-emptying valve, 17-buffer solution pool, 18-reagent pool, 19-dosing pool, 2-temperature control device and 3-detection device.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Example 1
The African swine fever virus micro-fluidic detector comprises a micro-fluidic chip 1, a temperature control device 2 and a detection device 3, wherein the temperature control device 2 and the detection device 3 are respectively connected with the micro-fluidic chip 1, and the micro-fluidic chip 1 comprises a sample inlet area 11, a sample processing area 12, a detection area 13 and a sample outlet area 14 which are sequentially communicated; the sample inlet area 11 is provided with a quantitative ring 15, and the sample inlet area 11 is communicated with the sample processing area 12 through the quantitative ring 15; the detection area 13 comprises a positive control area 131, a blank control area 133 and a reaction area 134, biochemical reagents for detecting undetermined indexes are coated in the positive control area 131, the blank control area 133 and the reaction area 134, the sample processing area 12 is respectively communicated with the positive control area 131, the blank control area 133 and the reaction area 134, and the sample outlet area 14 is provided with an emptying valve 16; the micro-fluidic chip 1 also comprises a buffer solution pool 17, a reagent pool 18 and a dosing pool 19, wherein a buffer solution for processing a sample is arranged in the buffer solution pool 17, a reagent for processing the sample is arranged in the reagent pool 18, the buffer solution pool 17 and the reagent pool 18 are respectively communicated with the sample processing area 12, and the dosing pool 19 is communicated with the detection area 11; the sample processing area 12 and the detection area 13 are connected to the temperature control device 2, and the detection device 3 is connected to the detection area 13.
The detection device 3 is an enzyme-labeling instrument which is connected with the detection area 13 and used for detecting the signal of the solution in the detection area 13.
The detection zone 13 also includes a negative control zone 132, the negative control zone 132 being internally coated with a biochemical reagent for detecting an analyte of interest, the negative control zone 132 being in communication with the sample treatment zone 12.
The number of the sample inlet regions 11 is 3, the number of the sample outlet regions 14 is 1, the number of the positive control regions 131, the negative control regions 132 and the blank control regions 133 in the detection region 13 is 1, the number of the reaction regions 134 is 3, one sample inlet region (11) is used for each of the positive control regions 131 and the negative control regions 132, and the sample inlet region 11 and the sample outlet region 14 are shared by the blank control regions 133 and the reaction regions 134.
The positive control area 131, the blank control area 133, and the reaction area 134 and the negative control area 132 were 10mm in length and 6mm in width.
Example 2
Unlike example 1, in this example, the number of reaction regions 134 was 2, and the positive control region 131, the blank control region 133, the reaction regions 134 and the negative control region 132 were 8.5mm in length and 5mm in width.
Example 3
In the same manner as in example 1, except that the positive control zone 131, the blank control zone 133, the reaction zone 134 and the negative control zone 132 in this example were 8.5mm in length and 6mm in width.
In short, the above description is only a preferred embodiment of the present invention, and all the equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the scope of the present invention.
Claims (5)
1. African swine fever virus micro-fluidic detector, including micro-fluidic chip (1), temperature control device (2), detection device (3), its characterized in that: the temperature control device (2) and the detection device (3) are respectively connected with the microfluidic chip (1), and the microfluidic chip (1) comprises a sample inlet area (11), a sample processing area (12), a detection area (13) and a sample outlet area (14) which are sequentially communicated; the sample introduction area (11) is provided with a quantitative ring (15), and the sample introduction area (11) is communicated with the sample processing area (12) through the quantitative ring (15); the detection area (13) comprises a positive control area (131), a blank control area (133) and a reaction area (134), biochemical reagents for detecting undetermined indexes are coated in the positive control area (131), the blank control area (133) and the reaction area (134), the sample processing area (12) is respectively communicated with the positive control area (131), the blank control area (133) and the reaction area (134), and the sample outlet area (14) is provided with an emptying valve (16); the micro-fluidic chip (1) also comprises a buffer solution pool (17), a reagent pool (18) and a dosing pool (19), wherein a buffer solution for processing a sample is arranged in the buffer solution pool (17), a reagent for processing the sample is arranged in the reagent pool (18), the buffer solution pool (17) and the reagent pool (18) are respectively communicated with the sample processing area (12), and the dosing pool (19) is communicated with the detection area (13); the sample processing area (12) and the detection area (13) are respectively connected with the temperature control device (2), and the detection device (3) is connected with the detection area (13).
2. The microfluidic detector of African swine fever virus according to claim 1, wherein: temperature control device (2) includes heating device and temperature sensor, and heating device is connected with sample processing district (12) and detection zone (13), and heating device is used for heating solution in sample processing district (12) and detection zone (13), and temperature sensor is used for detecting the temperature of solution in sample processing district (12) and detection zone (13).
3. The microfluidic detector of African swine fever virus according to claim 1, wherein: the detection device (3) is an enzyme-labeling instrument, and the enzyme-labeling instrument is connected with the detection area (13) and is used for detecting signals of the solution in the detection area (13).
4. The microfluidic detector of African swine fever virus according to claim 1, wherein: the detection zone (13) further comprises a negative control zone (132), the negative control zone (132) is internally coated with a biochemical reagent for detecting an index to be determined, and the negative control zone (132) is communicated with the sample processing zone (12).
5. The microfluidic detection instrument for African swine fever virus according to claim 4, wherein: the positive control area (131), the blank control area (133), the reaction area (134) and the negative control area (132) are 8.5-10 mm in length and 5-6 mm in width.
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| CN201920617118.7U CN210347665U (en) | 2019-04-30 | 2019-04-30 | African swine fever virus micro-fluidic detector |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112169853A (en) * | 2020-12-01 | 2021-01-05 | 南京岚煜生物科技有限公司 | Multifunctional microfluidic detection chip |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112169853A (en) * | 2020-12-01 | 2021-01-05 | 南京岚煜生物科技有限公司 | Multifunctional microfluidic detection chip |
| CN112169853B (en) * | 2020-12-01 | 2021-02-26 | 南京岚煜生物科技有限公司 | Multifunctional microfluidic detection chip |
| WO2022116758A1 (en) * | 2020-12-01 | 2022-06-09 | 南京岚煜生物科技有限公司 | Multi-functional microfluidic test chip |
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