CN216209190U - Rapid detection structure for liquid phase sample sampling and detection - Google Patents
Rapid detection structure for liquid phase sample sampling and detection Download PDFInfo
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- CN216209190U CN216209190U CN202122395917.4U CN202122395917U CN216209190U CN 216209190 U CN216209190 U CN 216209190U CN 202122395917 U CN202122395917 U CN 202122395917U CN 216209190 U CN216209190 U CN 216209190U
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Abstract
The utility model discloses a rapid detection structure for sampling and detecting a liquid phase sample, which consists of a detection structure and a sampling structure which is combined with the detection structure for use and can form detachable connection, wherein the detection structure consists of a detection buckle and a reagent strip positioned in the detection buckle, the sampling structure is an integrated structure provided with a sampling head and a sampling handle, the sampling head consists of a fixed structure and a sampling swab arranged on the fixed structure, the sampling swab is a water absorption material which is changed from hard to soft after absorbing water and is used as an indicator for judging a sampling end point, the detection buckle is provided with a sampling connection structure, the side surface of the far-end groove wall of the detection buckle is provided with a lever fulcrum, and the bottom of the groove is provided with an opening structure which is in direct liquid phase communication with the reagent strip. The utility model can be used for developing various rapid immunoassay products such as colloidal gold, fluorescence immunoassay and the like, improves the detection efficiency, convenience and accuracy of the immunoassay products, and has important clinical significance.
Description
Technical Field
The utility model relates to the technical field of medical instruments, in particular to a rapid detection structure for sampling and detecting a liquid phase sample and application thereof.
Background
The immunological detection technology is an experimental means for determining antigens, antibodies, immune cells, chemical components and the like by applying the immunological principle, and is widely applied to samples which are derived from human bodies and animal bodies and can be used for disease diagnosis and health detection and samples for environmental, pharmaceutical, food and industrial analysis. Commonly used are immunoturbidimetry, solid-phase enzyme immunoassay, chemiluminescence detection, immunofluorescence labeling, quantum dot immunoassay, colloidal gold immunoassay, spot immunoassay, etc. Among them, point-of-care testing (POCT) is the branch of the fastest development, immunochromatography testing is the most common testing method, and colloidal gold and fluorescence lateral flow chromatography immunoassay technology products are most widely used. High sensitivity, rapidness, convenience, miniaturization, full quantification and automation are the development trends and the development efforts of technical innovation, and are being perfected by various technical innovations and technical improvements. The adopted samples to be detected comprise liquid phase samples such as saliva, blood, urine, various secretions and the like, wherein the blood is used most, most comprehensively and most typically, but the samples have certain inconvenience due to interventional sampling. In recent years, saliva detection is increasingly widely used due to the convenience of sampling, and a plurality of blood biochemical indexes are changed and infectious pathogen markers are also shown in saliva, so that a more convenient detection means is provided for diagnosis of various diseases, particularly for primary screening. The currently adopted liquid phase collection method to be detected is generally two or more steps, such as sampling, dilution extraction, sample adding, observation and the like, so that a detection technology which is more convenient, faster and higher in performance and can save the dilution extraction step is developed, the popularization and the use of clinical detection products are facilitated, the diagnosis and treatment quality is improved, and the method has an important application value.
Disclosure of Invention
Compared with the prior art, the rapid detection structure for sampling and detecting the liquid phase sample and the application thereof have the characteristics of convenience, rapidness, direct sample adding and the like, and effectively improve the detection quality and the user acceptance degree.
In order to achieve the above object, the present invention provides a rapid detection structure for sampling and detecting a liquid phase sample, which comprises a detection structure and a sampling structure combined with the detection structure and capable of forming a detachable connection, wherein the detection structure is an immunochromatography detection structure and comprises a detection buckle and a reagent strip located in the detection buckle, the reagent strip arranged from a proximal end to a distal end of the detection buckle is a test strip sample structure, and sequentially comprises a sample pad, a marker binding pad, a nitrocellulose membrane pad and a water absorption paper pad, and the detection buckle is provided with an observation window above the nitrocellulose membrane pad, and is characterized in that:
the near end of the detection buckle is provided with a sampling connection structure with a groove-shaped structure, wherein the near end of the reagent strip is arranged below the sampling connection structure;
the sampling structure is an integrated structure provided with a sampling head and a sampling handle, the sampling head consists of a fixed structure and a sampling swab arranged on the fixed structure, the sampling swab is made of a water absorption material and absorbs a sample, and a lever supporting arm is arranged at the top end of the fixed structure;
the water absorption material is a material which changes from hard to soft after absorbing water, and the indicator of the sampling end point of the sampling swab is that the water absorption material changes from hard to soft;
a lever fulcrum is arranged on the side face of the far end of the groove wall of the sampling connection structure, and an opening structure which forms direct liquid phase communication with the reagent strip is arranged at the bottom of the groove;
the sampling head is detachably inserted into the groove-shaped structure of the sampling connecting structure, and a sample to be detected is transferred to the reagent strip through the opening structure.
Furthermore, the sampling connection structure is a groove-shaped structure with an upward opening, and the opening structure arranged at the bottom is arranged at the near end of the bottom and corresponds to the sample pad part of the reagent strip. The opening structure is arranged at the position 1/2-1/3 at the proximal end of the bottom in the groove-shaped structure.
Furthermore, the near end of the sampling connecting structure is provided with an extrusion fixing structure for detachably fixing the sampling handle.
Further, the fulcrum of the sampling connection structure has at least one of the following characteristics:
1) a recessed structure disposed on an inner wall of the distal end of the slot wall; at the moment, a lever supporting arm arranged at the top end of the fixing structure of the sampling connection structure is inserted into the sunken structure to realize the fixed support of the lever head;
2) a raised structure disposed on an inner wall of the distal end of the slot wall; at the moment, a lever supporting arm arranged at the top end of the fixing structure of the sampling connection structure is inserted below the bulge structure to realize the fixed support of the lever head;
3) a through-wall open cell structure disposed at a distal end of the cell wall; at the moment, a lever supporting arm arranged at the top end of the fixing structure of the sampling connection structure is inserted into the through-wall opening structure to realize the fixed support of the lever head;
4) the bracket type structure is arranged on the inner wall of the far end of the groove wall and is a lever riveting supporting structure containing a lower support and an upper support. At the moment, the lever supporting arm arranged at the top end of the fixing structure of the sampling connection structure is inserted into the lever riveting supporting structure, the lower support is used as a lower fulcrum, the upper support is used as an upper fulcrum, and the fixed support of the lever head is realized.
Furthermore, the water absorbing material which changes from hard to soft after absorbing water is one or more of sponge products and polyvinyl alcohol products. The water absorbing material is mainly natural and modified high molecular high water absorbing resin and artificially synthesized water absorbing resin, including starch series, cellulose series, other natural product series, polyvinyl acid salt series, polyvinyl alcohol series, polyoxyethylene series, etc. and partial polyvinyl alcohol series product has the features of hard drying and soft absorbing water. The polyvinyl alcohol (PVA) water-absorbing sponge not only has excellent chemical stability, water-absorbing function and high-quality physical property, but also has the characteristics of high water-absorbing rate, high liquid-absorbing rate, softness after water absorption and the like, and is widely used in the fields of daily cleaning, medical treatment and health care and the like.
Further, the detection reagent strip is at least one of a reagent strip prepared by a colloidal gold immunoassay method and a reagent strip prepared by a fluorescence immunoassay method. The structure of the kit sequentially comprises a sample pad, a marker combination pad, a nitrocellulose membrane pad and a water absorption paper pad from near to far. The sample pad is used for adsorbing a sample to be detected and pretreating the sample to be detected before detection, the marker combining pad is used for coating a marker, a colloidal gold method is a colloidal gold labeled antibody or antigen, a fluorescence immunoassay method is a fluorescence labeled antibody or antigen, the nitrocellulose membrane pad is coated with the antibody or antigen matched with the coated antibody or antigen on the marker combining pad, and the absorbent paper pad is a fiber product with good water absorption performance and assists the sample to be detected to flow on the reagent strip.
Furthermore, the lever riveting support structure is a structure that the lower support and the upper support are arranged in a staggered manner, the lower support is arranged at the near end, the upper support is arranged at the far end, and the upper edge of the lower support and the lower edge of the upper support are arranged on the same plane.
Further, the sample collected by the sampling structure includes at least one of saliva, urine, blood, and secretion.
Further, the operation of the rapid detection structure comprises the following steps:
1) the sampling head is extended into the sampling part, and the sample is sucked by the sampling swab to finish sampling;
2) inserting the sampling swab into the sampling connection structure in a mode that the sampling swab faces the bottom surface of the groove-shaped structure, and butting a lever supporting arm positioned at the top end of the fixing structure with a lever fulcrum of the sampling connection structure;
3) the sampling handle is pressed towards the bottom surface of the groove-shaped structure, a sample sucked by the sampling swab flows into the detection reagent strip through the opening structure and then flows through the combination pad, the nitrocellulose membrane pad and the absorbent paper pad, and detection is finished;
4) and reading a detection result from the observation window of the detection buckle.
During operation, the sampling handle is pressed downwards to a lever fulcrum located at the far end of the groove-shaped structure is used as a support, a liquid phase sample absorbed in the sampling swab is extruded to flow out of the sampling swab, flows into the detection reagent strip sample pad through the outlet structure, is subjected to adsorption pretreatment, then flows through the combination pad, is combined with the coated labeled antibody or antigen, flows into the nitrocellulose membrane pad, is captured by the coated corresponding antibody or antigen, and the absorbent paper pad attracts and drives a liquid phase to flow on the reagent strip, so that the detection result is read, and the detection is completed.
Due to the adoption of the technical scheme, the utility model has the following advantages:
1. the utility model adopts a rapid detection structure of split sampling and combined integrated detection, so that the sampling head is not in direct contact with the detection reagent strip during sampling, thereby avoiding the pollution of components on the detection reagent strip to a detected person, simultaneously ensuring the direct contact between the sampling head and the detection reagent strip during detection reaction, not only improving the convenience from sampling to detection operation of a clinical sample, but also effectively avoiding the interference of other factors on the sample detection, and improving the detection quality.
2. The utility model adopts the water absorbing material which is changed from hard to soft after absorbing water as the sampling swab, thereby not only improving the comfort of the sampling process, but also realizing the perceptibility of the detected person to the sampling process, controlling the completion condition of the sampling by perceiving the softening degree of the sampling swab, namely being used as an indicator for judging the completion of the sampling, and improving the success rate of the sampling.
3. According to the utility model, the lever principle structure is arranged in the sampling connection structure, so that a sample to be detected absorbed to the sampling swab is transferred to the detection reagent strip for detection through the lever squeezing effect, the convenience of detection operation is improved, the speed of the sample to be detected entering the sample pad and flowing through the combination pad is effectively promoted, the detection process is accelerated, and the detection success rate and the detection speed are improved.
4. The utility model adopts the detection test strip as a detection part, is suitable for various detection technologies which take lateral flow as main technical characteristics, such as colloidal gold, fluorescence immunity and the like, and expands the application range of the detection technology.
5. The method has simple operation steps, is easy to realize household use or self-operation, is convenient to use, reduces the waste of raw materials, obviously improves the working efficiency, and is applied to various fields of specialty and amateur detection.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of a detection structure of the present invention
FIG. 3 is a schematic view of a sampling connection according to the present invention;
FIG. 4 is a schematic diagram of a sampling structure of the present invention;
FIG. 5 is a schematic view of a sampling head structure according to the present invention;
FIG. 6 is a schematic view of the detachable structure of the present invention;
FIG. 7 is a schematic view of a lever riveting support structure according to the present invention;
FIG. 8 is a schematic longitudinal sectional view of the detachable connection of the present invention;
FIG. 9 is a schematic view of the structure of the test strip of the present invention.
The figures are labeled as follows:
a detection structure 1; a sampling structure 2; a sampling connection structure 3; a sampling head 4; a sampling handle 5; a pressing and fixing structure 6; an observation window 7; a detection reagent strip 8; the upper cover 9 is detected; a detection base 10; a recessed lever fulcrum 11; a sampling swab 12; a fixed structure 13; a channel structure 14; a lever support arm 15; an opening structure 16; a lower support 17; an upper support 18; a sample pad 19; a label-binding pad 20; a nitrocellulose membrane mat 21; a water absorbent paper pad 22.
Detailed Description
To further illustrate the technical means and effects of the present invention for achieving the predetermined purpose, the following embodiments are further described with reference to the accompanying drawings, but the present invention is not limited to the following description.
As shown in fig. 1, the overall structure of the utility model comprises a detection structure 1, a sampling structure 2, a sampling connection structure 3, a sampling head 4, a sampling handle 5, an extrusion fixing structure 6 and an observation window 7, and is a rapid detection structure for liquid phase sample sampling and detection, which is composed of the detection structure 1 and the sampling structure 2 which is used in combination with the detection structure and can form a detachable connection.
As shown in fig. 2, the detecting structure of the present invention includes a sampling connecting structure 3, an extruding fixing structure 6, an observation window 7, a detecting reagent strip 8, a detecting upper cover 9, a detecting base 10, a recessed lever fulcrum 11, a groove-shaped structure 14, and an opening structure 16, wherein the detecting reagent strip 9 is placed on the detecting base 10, the detecting upper cover 9 is provided with the observation window 7, the reaction condition of the detecting reagent strip 8 can be observed through the observation window 7, and the extruding fixing structure 6, the recessed lever fulcrum 11, the groove-shaped structure 14, and the opening structure 16 are combined components of the sampling connecting structure 3, and are used for detecting and transmitting a test sample.
As shown in fig. 3, 4 and 5, the sampling structure of the present invention comprises a sampling head 4, a sampling handle 5, a sampling swab 12, a fixing structure 13 and a lever supporting arm 15, wherein the sampling head 4 is fixed on the sampling handle 5 through the sampling head fixing structure 13, and the sampling head 4 is placed on a sampling site for sampling by holding the sampling handle 5 with a hand during operation. If a saliva sample is to be taken, the sampling head 4 is placed in the mouth. The sampling head 4 is placed in the blood if a blood sample is to be taken. If a urine sample is to be collected, the sampling head 4 is placed in the urine. The sampling swab 12 is made of a water-absorbing material which changes from hard to soft after absorbing water, and one or more of a sponge product and a polyvinyl alcohol product are selected. The polyvinyl alcohol (PVA) water-absorbing sponge not only has excellent chemical stability, water-absorbing function and excellent physical properties, but also has the characteristics of high water-absorbing rate, high liquid-absorbing rate, softness after water absorption and the like, and is one of the choices of the utility model. By adopting the water absorbing material which is changed from hard to soft after absorbing water as the sampling swab, the comfort of the sampling process is improved, the perceptibility of a detected person to the sampling process is realized, the completion condition of sampling is controlled by sensing the softening degree of the sampling swab, namely, the sampling swab is used as an indicator for judging the completion of sampling, and the success rate of sampling is improved. The lever support arm 15 is a squeezing support arm for the sampling structure to transfer the sample to be examined to the detection structure 1.
As shown in fig. 6 and 8, in the detachable connection structure of the present invention, the sampling structure 2 and the detection structure 1 are separated from each other before sampling, after sampling, the sampling swab 12 of the sampling head is inserted into the groove structure 14 of the sampling connection structure, the sampling swab 12 is squeezed by pressing down the sampling handle 5, the sampling handle 5 is locked in a pressed-down state by the squeezing and fixing structure 6, so that the collected sample to be detected flows into the detection reagent strip 8 located below the sampling swab through the opening structure 16, and the detection reaction is started and completed.
As shown in fig. 7 and 8, the lever riveting support structure of the present invention comprises a groove structure 14, an opening structure 16, a lower support 17 and an upper support 18, wherein the lever support arm disposed at the top end of the fixing structure of the sampling head is inserted into the lever riveting support structure, the lower support 17 is used as a lower fulcrum, the upper support 18 is used as an upper fulcrum, and the fixing support of the lever head and the effective downward pressing action of the sampling swab 12 are realized.
As shown in fig. 9, the detection reagent strip structure of the present invention is disposed on the detection base, and sequentially comprises a sample pad 19, a marker combination pad 20, a nitrocellulose membrane pad 21 and a water absorbent paper pad 22 from near to far, wherein the corresponding structure above the sample pad 19 corresponds to the opening structure 16, and the nitrocellulose membrane pad 21 corresponds to the observation window 7. The sample pad is used for adsorbing a sample to be detected and pretreating the sample to be detected before detection, the marker combining pad is used for coating a marker, a colloidal gold method is a colloidal gold labeled antibody or antigen, a fluorescence immunoassay method is a fluorescence labeled antibody or antigen, the nitrocellulose membrane pad is coated with the antibody or antigen matched with the coated antibody or antigen on the marker combining pad, and the absorbent paper pad is a fiber product with good water absorption performance and assists the sample to be detected to flow on the reagent strip.
Thus, in practical operation, when the rapid detection structure of the utility model is a colloidal gold immunoassay structure, the detection reagent strip 8 is a test strip prepared by a colloidal gold method, and a sample pad 19, a colloidal gold conjugate pad 20 coated with a colloidal gold marker, a nitrocellulose membrane pad 21 coated with a non-labeled capture reagent and a water absorbent paper pad 22 are sequentially adhered on the PVC support plate; when the rapid detection structure is a fluorescence immunoassay structure, the detection reagent strip 8 is a test strip prepared by a fluorescence immunoassay method, and a sample pad 19, a fluorescent microsphere bonding pad 20 coated with a fluorescent marker, a nitrocellulose membrane pad 21 coated with a non-marker capture reagent and a water absorption paper pad 22 are sequentially adhered on a PVC support plate.
When in use, the detection reagent card is taken out, the sampling structure 2 is taken out, the sampling handle 5 is held by hand, the sampling head 4 is used for collecting a sample, then the sampling head 4 is put into the sampling connecting structure 3 on the detection reagent card, the lever supporting arm 15 positioned on the sampling head 4 is butted with the lever fulcrum 11 on the far end wall of the groove-shaped structure 14, the sampling handle 5 is pushed downwards, the sampling swab 12 is extruded in the groove-shaped structure 14, the sampling handle is locked to be in a downward pressing state by the extruding and fixing structure 6, the still standing is carried out for 10 to 30 minutes, the collected sample flows into the sample pad 19 of the detection reagent strip 8 through the opening structure 16, the sample is pretreated by the sample pad, flows into the marker combination pad 20, the object to be detected is combined with the marker, flows into the cellulose nitrate membrane pad 21, the object to be detected combined with the marker is captured, the corresponding colloidal gold color development or fluorescence is generated, the result is read, and finishing the detection.
Experimental study of the utility model: the following experiment is illustrative of the detection method of the present invention and its effects, but is not intended to limit the present invention. The experimental methods used in the following experiments are all conventional methods unless otherwise specified. The materials, reagents and the like used are commercially available unless otherwise specified.
Experiment one: the utility model relates to an immune colloidal gold method new coronavirus antigen rapid detection experiment:
firstly, preparing a detection reagent strip:
preparing a detection reagent strip by adopting a conventional immune colloidal gold detection technology and a double-antibody sandwich method, and preparing a detection kit by adopting the rapid detection structure to perform a new coronavirus antigen detection experiment, wherein a colloidal gold label of a detection line T of the detection reagent strip indicates that an antibody is an anti-new coronavirus S protein monoclonal antibody of 10ug/ml, and colloidal gold particles with the particle size of 50nm are coated on a glass cellulose membrane colloidal gold conjugate pad; the capture antibody of the detection line T of the detection reagent strip is a 1.0mg/ml paired anti-new coronavirus S protein monoclonal antibody, and the capture antibody is coated on a nitrocellulose membrane; the capture antibody of the quality control line C of the detection reagent strip is a goat anti-mouse IgG polyclonal antibody of 1.0mg/ml, and the capture antibody is coated on a nitrocellulose membrane and used for capturing the colloidal gold labeled anti-new coronavirus S protein monoclonal antibody which is not captured specifically. And respectively sticking a water absorption paper membrane pad and a colloidal gold mark combined membrane pad at two ends of the nitrocellulose membrane printing membrane, and sticking a sample pad at one side of the combined membrane pad. And placing the adhered detection sheet on a strip cutting machine, and cutting into 3.5mm test strips.
Secondly, preparing a rapid detection structure:
the upper cover, the base and the sampling structure of the rapid detection structure are designed by Solidworks, a sample is printed in a 3D mode, a polyvinyl alcohol absorbent sponge sampling swab is pasted on the sampling head fixing structure, and the sample is prepared for experimental detection.
Third, experimental method and result:
during the experiment, the prepared detection reagent strip and the rapid detection structure are assembled into a detection buckle, the assembled detection buckle is placed into an aluminum amber sealing bag with a drying agent, the sealing is carried out on a sealing machine, and a label is added. Preparing recombinant new coronavirus antigen S protein solutions with different concentrations by using healthy human saliva. Taking out the sampling structure, soaking the sampling head with the prepared solution, then placing the sampling head in the groove-shaped structure, downwards extruding the sampling handle and inserting the sampling handle into the detection buckle card through the insertion hole, locking the sampling handle in a downwards-pressed state by using the extrusion fixing structure, standing for 20 minutes, observing a detection window, and reading a color development result on the test strip. The quality control line C of the test strip is colored, and the test line T is not colored and is negative; the quality control line C is colored, and the detection line T is also colored and is positive. As a result, positive reactions were observed at antigen S protein solution concentrations of 1.0, 0.1 and 0.05ng/ml, and negative reactions were observed at concentrations of 0.01ng/ml or less.
Experiment two: the utility model relates to an immunofluorescence method new coronavirus antigen rapid detection experiment, which comprises the following steps:
firstly, preparing a detection reagent strip:
preparing a detection reagent strip by adopting a conventional immunofluorescence detection technology double-antibody sandwich method, and preparing a detection kit by adopting the rapid detection structure to perform a new coronavirus antigen detection experiment, wherein fluorescent microspheres in a detection line T of the detection reagent strip mark an anti-new coronavirus S protein monoclonal antibody with an indication antibody of 20ug/ml, and the fluorescent microspheres with the particle size of 50 mu m are coated on a cellophane film colloidal gold combination pad; the capture antibody of the detection line T of the detection reagent strip is a 1.0mg/ml paired anti-new coronavirus S protein monoclonal antibody, and the capture antibody is coated on a nitrocellulose membrane; the capture antibody of the quality control line C of the detection reagent strip is a goat anti-mouse IgG polyclonal antibody of 1.0mg/ml, and the capture antibody is coated on a nitrocellulose membrane and used for capturing the fluorescent microsphere labeled anti-new coronavirus S protein monoclonal antibody which is not captured specifically. And respectively sticking a water absorption paper membrane pad and a fluorescent microsphere mark combined membrane pad at two ends of the nitrocellulose membrane printing membrane, and sticking a sample pad at one side of the combined membrane pad. And placing the adhered detection sheet on a strip cutting machine, and cutting into 3.5mm test strips.
Secondly, preparing a rapid detection structure:
prepared as in experiment one.
Thirdly, a fluorescence detector: a commercially available blue Bo AFS-100 fluorescence detector was used.
Fourthly, experimental method and result:
during the experiment, the prepared detection reagent strip and the rapid detection structure are assembled into a detection buckle, the assembled detection buckle is placed into an aluminum amber sealing bag with a drying agent, the sealing is carried out on a sealing machine, and a label is added. Preparing recombinant new coronavirus antigen S protein solutions with different concentrations by using healthy human saliva. Taking out the sampling structure, soaking the sampling head with the prepared solution, then placing the sampling head in the groove-shaped structure, downwards extruding the sampling handle, locking the sampling handle in a downwards-pressed state by using the extruding and fixing structure, standing for 20 minutes, placing the detection buckle in a fluorescence detector, starting detection, and reading a fluorescence test result on the test strip. The ratio of the fluorescence value of the detection line T on the detection test strip to the fluorescence value of the quality control line C is more than 0.02, and the detection test strip is positive; the ratio of the fluorescence value of the detection line T on the detection test strip to the fluorescence value of the quality control line C is less than or equal to 0.02, and the detection test strip is negative. As a result, the antigen S protein solution was found to be positive at concentrations of 1.0, 0.1, 0.05 and 0.01ng/ml, and negative at concentrations of 0.005ng/ml or less.
Claims (6)
1. The utility model provides a short-term test structure for liquid phase sample sampling and detection, by detect the structure and with it uses and can form the sampling structure component that detachable connects to detect the structure combination, it detects the structure to detect the structure for immunochromatography, detains the card and is located by the detection detect the reagent strip in detaining the card and constitute, it sets up from near-end to distal end to detect detain the card the reagent strip is test paper strip appearance structure, includes sample pad, marker combination pad, nitrocellulose membrane pad and the paper pad that absorbs water in proper order, it is provided with observation window, its characterized in that to detect the knot card in the top that nitrocellulose membrane pad corresponds:
the near end of the detection buckle is provided with a sampling connection structure with a groove-shaped structure, wherein the near end of the reagent strip is arranged below the sampling connection structure;
the sampling structure is an integrated structure provided with a sampling head and a sampling handle, the sampling head consists of a fixed structure and a sampling swab arranged on the fixed structure, the sampling swab is made of a water absorption material and absorbs a sample, and a lever supporting arm is arranged at the top end of the fixed structure;
the water absorption material is a material which changes from hard to soft after absorbing water, and the indicator of the sampling end point of the sampling swab is that the water absorption material changes from hard to soft;
a lever fulcrum is arranged on the side face of the far end of the groove wall of the sampling connection structure, and an opening structure which forms direct liquid phase communication with the reagent strip is arranged at the bottom of the groove;
the sampling head is detachably inserted into the groove-shaped structure of the sampling connecting structure, and a sample to be detected is transferred to the reagent strip through the opening structure.
2. The rapid test structure according to claim 1, wherein the sampling connection structure is a groove structure with an upward opening, and the opening structure disposed at the bottom is disposed at the proximal end of the bottom and corresponds to the sample pad portion of the reagent strip.
3. The rapid detection structure of claim 1, wherein the proximal end of the sampling connection structure is provided with a press-fit structure that removably secures the sampling handle.
4. The rapid sensing architecture of claim 1, wherein the fulcrum of the sampling connection architecture has at least one of the following characteristics:
1) a recessed structure disposed on an inner wall of the distal end of the slot wall;
2) a raised structure disposed on an inner wall of the distal end of the slot wall;
3) a through-wall open cell structure disposed at a distal end of the cell wall;
4) the bracket type structure is arranged on the inner wall of the far end of the groove wall and is a lever riveting supporting structure containing a lower support and an upper support.
5. The rapid test structure according to claim 1, wherein the water-absorbing material that changes from hard to soft after absorbing water is selected from a polyvinyl alcohol product.
6. The rapid test structure according to claim 1, wherein the reagent strip is at least one of a reagent strip prepared by a colloidal gold immunoassay and a reagent strip prepared by a fluorescence immunoassay.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2024046296A1 (en) * | 2022-08-29 | 2024-03-07 | 利多(香港)有限公司 | Test kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024046296A1 (en) * | 2022-08-29 | 2024-03-07 | 利多(香港)有限公司 | Test kit |
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