CN1700006A - Avian influenza virus piezoelectric biological sensor detecting instrument - Google Patents
Avian influenza virus piezoelectric biological sensor detecting instrument Download PDFInfo
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- CN1700006A CN1700006A CN 200510057022 CN200510057022A CN1700006A CN 1700006 A CN1700006 A CN 1700006A CN 200510057022 CN200510057022 CN 200510057022 CN 200510057022 A CN200510057022 A CN 200510057022A CN 1700006 A CN1700006 A CN 1700006A
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Abstract
The invention relates to a piezoelectric crystal sensor with specially treated and filmed macromolecular film and combined with AIV monoclonal antibody, which uses data colleting system and relicon together to do qualitative and quantitative measure to AIV. It has the following characters: AIV piezoelectric biosensor's probe crystal is though specially treated and it can be used as piezoelectric sensor signal transformer to test sample. Measuring device comprises piezoelectric sensor (1), MCU unit (2), relicon display LCD (3) and continuous temperature measuring pool (4). Piezoelectric sensor (1) is connected with MCU unit (2) though frequency oscillating blocks (6), while MCU unit (2) is connected with LCD (3). The power of measuring system is provided by battery, LCD displays the outcome and its relation curve.
Description
One technical field
The present invention has created a kind of new avian influenza virus method for quick and detector thereof, is the result that immunology, macromolecular material are learned and biological doctor's electricity merges, and it is multidisciplinary that technical field belongs to.Specificity and the effective combination of macromolecular material and the susceptibility of piezoelectric crystal that it utilizes antigen-antibody reaction are mainly used in the animal epidemic prevention quarantine departments.
Two background technologies
The main at present technical method that adopts of avian flu virus detection has: the isolation identification of HA, HI, ELISA, agar gel diffusion test, immunofluorescence technique, RT-PCR, fluorescence RT-PCR and virus etc.Technology is ELISA and fluorescence RT-PCR more fast and accurately, but these 2 kinds of technology all need special instrument and equipment, and testing process complexity, expense height, detection time are all more than 2 hours.All these technical methods all can not be produced in basic unit, quarantine station and on-the-spot use the such as clinical, and special laboratory must be arranged.
The piezoelectric quartz crystal biology sensor is to utilize the piezoelectric quartz crystal oscillation frequency to plane of crystal mass loading and determinand proterties such as density, viscosity, electricity are led, the high susceptibility of specific inductive capacity etc. combines and grows up with the high degree of specificity of biological identification molecule a kind of novel sensor.It has highly sensitive, specificity good, simple to operate, do not need any mark, characteristics such as detection speed is fast, with low cost, can detect in real time.In recent years, piezoelectric quartz crystal sensor is used widely in fields such as field mobile operation such as medical experiment diagnosis, military monitoring, environment measuring, inspection for food hygiene, commercial production monitoring in real time and online detections, but the present report and relevant patent that the piezoelectric biological sensor detecting instrument device of avian influenza virus is not arranged as yet.
This invention is handled piezoelectric quartz crystal according to the sensitivity characteristic of piezoelectric biological sensor with macromolecular material, in conjunction with the AIV monoclonal antibody, make this sensor AIV have high degree of specificity.Simultaneously in conjunction with respective detection unit, configuration data disposal system and LCD, make that this instrumentation is simple, sensitivity is high, the response time is fast, can go out the result in general 20-30 minute, can be applied to the quick diagnosis and the quarantine of basic unit and clinical bird flu.
Three summary of the invention
This invention is a kind of technical method of new fast detecting avian influenza virus, have simple, fast, characteristics such as sensitivity and specificity be good, and cheap, be applicable to basic unit or clinical detection avian influenza virus.
This invention relates generally to the content of the following aspects:
1, avian influenza virus monoclonal antibody (AIV McAb) this be one of core of invention, utilize hybridoma technology to pass through Fusion of Cells, the avian influenza virus monoclonal antibody of the uniqueness that colony screening is set up, this antibody at the avian influenza virus reaction, is the McAb of avian influenza virus common epitope only.This antibody has determined the specificity of this instrument.On the quartz wafer of handling through macromolecular material, make the piezoelectric biological sensor probe with this antibody labeling, the AIV specific bond in this sensor and the sample to be checked is by AIV in instrument data disposal system and the accurate response sample of LCD.
2, the piezoelectric sensor wafer of instrument is handled with macromolecular material, and the macromolecular material aldehyde radical is connected with the AIV monoclonal antibody by connecting chemicals, can preserve separately, is convenient to large-scale production and use.
3, instrument of the present invention combines with LCD by AIV piezoelectric biological sensor, hunting of frequency module, standard second signal circuit, MCU unit.The AIV piezoelectric sensor is put into liquid to be detected, and piezoelectric sensor is connected with the MCU unit by the hunting of frequency module, links to each other with LCD again, and the power supply of whole detection system is provided by battery, and the result and the correlation curve of detection are shown in real time by LCD.
Four specific embodiments
Following example just illustrates the science and the operability of this invention and proves susceptibility and the specificity that this instrument has, and can not include all the elements of invention, concrete this scope that is not limited to of using.
Embodiment 1: with the avian influenza virus standard antigen of deactivation with normal not immune chicken serum by 1: 10-1: 10000 dilutions, detect with the AIV piezoelectric biological sensor that assembles.The positive sample oscillation frequency significantly raises, and tends to be steady in 30 minutes, and the oscillation frequency of negative sample does not have marked change, and testing process is in the comparison plateau always.Can judge the positive and feminine gender according to the frequency change situation.The greatest dilution that can detect positive sample is 1: 12000, and the HA test can only detect 1: 100.Dilute newcastle disease virus (NDV) weakness vaccine with chicken serum simultaneously, infectious bursa diseases virus, this instrument detecting contains these viral chicken serums and normal chicken serum, and is all negative.Illustrate that this instrument has specificity and susceptibility preferably.
Embodiment 2: behind AIV inactivation antigen purifying, measure protein concentration with ultraviolet spectrophotometer, increase progressively dilution with 10 times of normal not immune chicken serum then and become 100ug/ml, 10ug/ml, 1ug/ml, 100ng/ml, 50ng/ml, 25ng/ml, 10ng/ml, 1ng/ml, 0.1ng/ml with doubling dilution, simultaneously NDV is done same dilution, detect with this instrument.The concentration that instrument can detect AIV is 20ng/ml, and NDV and normal serum detection are all negative, and the concentration that the HA test can detect AIV is 100ug/ml.Thereby prove the susceptibility and the specificity of this instrument.
Embodiment 3: the system chart of this instrument as shown in Figure 1, system architecture is formed synoptic diagram such as Fig. 2, mainly by AIV piezoelectric biological sensor (1), hunting of frequency module (5), MCU unit (6), standard second signal circuit (7), LCD interface (8) is formed.The power supply of whole detection system is supplied with by battery.During test, to put into the detection cell (4) of liquid to be detected (9) to the piezoelectric biological sensor (1) of AIV sensitivity, piezoelectric sensor is converted to frequency signal with AIV concentration, by the hunting of frequency module coupling of being made up of high speed integrated operational amplifier and related resistors, electric capacity etc.; Frequency signal enters 0 mouthful of the interruption of MCU unit, and the interruption of the gate time of standard second signal by the MCU unit enters for 1 mouthful, and by the gate time of control criterion signal second, the MCU unit detects the frequency of hunting of frequency module output; Afterwards, will detect signal and be passed to the processing unit with MCU, MCU handles signal, and sends result to LCD by the LCD interface, finishes the demonstration of testing result and correlation curve, testing process synoptic diagram such as Fig. 3 by LCD.
Present embodiment is work like this: the start detection instrument, enter AIV piezoelectric biological sensor detection system, and instrument at first carries out self check, and running parameters such as port numbers are set, and waits to be tested; Piezoelectric sensor is put into solution to be measured,, in LCD, show test data simultaneously, and draw detection curve by MCU software collection and the whole data of record.Simultaneously, MCU can come control detection pond temperature by temperature sensor and diode temperature control module, to keep the temperature constant state of testing process.
Claims (5)
1 one kinds of micro-avian influenza virus (AIV) piezoelectric biological sensor fast detecting instrument, be primarily characterized in that: detector is made up of avian influenza virus piezoelectric biological sensor (1), data acquisition processing system (2) and LCDs (3) and Constant Temperature Detection pond (4).
2 a kind of micro-avian influenza virus piezoelectric biological sensor fast detecting instrument according to claim 1, its feature (1) is: the AIV piezoelectric biological sensor is to scribble one deck polymeric membrane after the quartz wafer surface treatment with piezoelectric sensor, is combined with the avian influenza virus monoclonal antibody then.The quartz wafer that is combined with the AIV monoclonal antibody is the probe of piezoelectric sensor, can conveniently unload.
The quartz wafer of 3 a kind of micro-its feature of avian influenza virus piezoelectric biological sensor fast detecting instrument according to claim 1 (1) AIV piezoelectric biological sensors adds staphylococcal protein A bag quilt with Ethanol Treatment and DDW cleaning-drying.
4 a kind of micro-avian influenza virus piezoelectric biological sensor fast detecting instrument according to claim 1, it is characterized in that: finish the collection and the processing of signal by data acquisition system (DAS) (2), the real-time demonstration of the result of detection and correlation curve is finished by 5 inches liquid crystal displays (3).
5 a kind of micro-avian influenza virus piezoelectric biological sensor fast detecting instrument according to claim 1 is characterized in that: the application in fast quantification detection AIV virus, 20～30 minutes required detection times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510057022 CN1700006A (en) | 2005-04-18 | 2005-04-18 | Avian influenza virus piezoelectric biological sensor detecting instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510057022 CN1700006A (en) | 2005-04-18 | 2005-04-18 | Avian influenza virus piezoelectric biological sensor detecting instrument |
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| CN1700006A true CN1700006A (en) | 2005-11-23 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101000340B (en) * | 2006-01-12 | 2011-01-19 | 深圳市赛尔生物技术有限公司 | Method of biochip for simultaneous testing avian influenza infection of human and fowls |
| CN101149383B (en) * | 2007-09-30 | 2012-07-18 | 浙江工商大学 | Device for detecting avian influenza virus antibody and detection method |
| CN105784846A (en) * | 2016-04-08 | 2016-07-20 | 重庆理工大学 | Newcastle disease virus piezoelectric immunosense detection method |
| CN114018985A (en) * | 2021-11-03 | 2022-02-08 | 中国计量大学 | Biological virus concentration detector based on internet of things technology |
-
2005
- 2005-04-18 CN CN 200510057022 patent/CN1700006A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101000340B (en) * | 2006-01-12 | 2011-01-19 | 深圳市赛尔生物技术有限公司 | Method of biochip for simultaneous testing avian influenza infection of human and fowls |
| CN101149383B (en) * | 2007-09-30 | 2012-07-18 | 浙江工商大学 | Device for detecting avian influenza virus antibody and detection method |
| CN105784846A (en) * | 2016-04-08 | 2016-07-20 | 重庆理工大学 | Newcastle disease virus piezoelectric immunosense detection method |
| CN105784846B (en) * | 2016-04-08 | 2018-10-09 | 重庆理工大学 | A kind of newcastle disease virus piezoelectric immuno-sensing detection method |
| CN114018985A (en) * | 2021-11-03 | 2022-02-08 | 中国计量大学 | Biological virus concentration detector based on internet of things technology |
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