CN102162814A - Quick detection card for acid orange II and detection method thereof - Google Patents

Quick detection card for acid orange II and detection method thereof Download PDF

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Publication number
CN102162814A
CN102162814A CN 201110141144 CN201110141144A CN102162814A CN 102162814 A CN102162814 A CN 102162814A CN 201110141144 CN201110141144 CN 201110141144 CN 201110141144 A CN201110141144 A CN 201110141144A CN 102162814 A CN102162814 A CN 102162814A
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detection
acid orange
orange ii
film
card
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CN 201110141144
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Chinese (zh)
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吴文晔
周坚
熊晓芳
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无锡安迪生物工程有限公司
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Abstract

The invention discloses a quick detection card for acid orange II and a detection method thereof, belonging to the technical field of non-edible materials illegally added in food. The quick detection card for acid orange II is characterized in that a shell is provided with a test strip, and a support back plate is successively stuck with a sample pad, a colloidal gold film or a latex particle film, a nitrocellulose film and a water adsorption film to form the quick detection card; the colloidal gold film is a glass fiber film labelled by an antibody colloidal gold containing an acid orange II resistant antibody, or the latex particle film is the glass fiber film labelled by latex particles containing an acid orange II resistant antibody; and the nitrocellulose film is provided with two detection bands, wherein one detection band contains an acid orange II protein conjugate, and the other detection band is a quality control band containing an anti-rabbit antibody or an anti-rat antibody. The quick detection method has the advantage that the content of the acid orange II in samples, such as marinating cooked foods, Capsicum frutescens, beverages and the like, can be directly detected with the immunology method. The quick detection card for acid orange II disclosed by the invention is easy to prepare, is convenient and quick to use and has an accurate result.

Description

一种酸性橙II快速检测卡及其检测方法 An acidic Orange II card and its rapid detection method for detecting

技术领域 FIELD

[0001] 本发明涉及一种酸性橙II快速检测卡及其检测方法,是一种检测酸性橙II的检测器具,属于食品中违法添加非食用物质检测技术领域。 [0001] The present invention relates to a card Acid Orange II rapid detection and detection method, a detection instrument detecting Acid Orange II, belonging to food law to add non-food substances technical field of detection.

背景技术 Background technique

[0002] 酸性橙II是一种偶氮类酸性工业染料,又名酸性金黄II、金橙II或酸性艳橙GR,俗名金黄粉。 [0002] Acid Orange II is an industrial acidic azo dyes, also known as Acid Orange II, Orange II or an acid gold Brilliant Orange GR, common name golden powder. 溶于水,乙醇。 Dissolved in water, ethanol. 主要用于蚕丝、羊毛的染色,也可染皮革、纸张、锦纶,能印花,还可用作指示剂。 Mainly used for dyeing silk, wool, may also be dyed leather, paper, nylon, printing can also be used as an indicator. 拨染性、勻染性好,染色牢度较差,适用于色泽鲜艳度高的绒线染色。 Dial dyeing, leveling, good, poor color fastness, suitable for dyeing wool with high bright color. 染色时遇铜离子趋向红暗,遇铁离子色泽浅暗。 In case of copper ions tends staining dark red, dark, light in color in case of iron.

[0003] 偶氮类染料本身无任何直接的致癌作用,但是在体内还原分解,可形成芳香胺化合物,芳香胺在体内经过代谢活动后与靶细胞作用可能使DNA发生变化,成为人体病变的诱发因素,对人体和动物有潜在的致癌性和致突变性。 [0003] The azo dye itself without any direct carcinogenic effect reductive decomposition in the body but may form an aromatic amine compound, aromatic amine after metabolic activity and action on target cells in vivo may cause changes in DNA, become a human disease-induced factors, potential carcinogenicity and mutagenicity to humans and animals. 由于其致癌性和致突变性,世界上很多国家对酸性橙II的使用实施了严格的规定。 Because of its carcinogenic and mutagenic, many countries in the world on the use of acid orange II of the implementation of strict rules. 在我国,酸性橙II禁止被用作食品添加剂。 In China, Acid Orange II prohibited from being used as a food additive.

[0004] 酸性橙II属非食用色素,食品中不允许加入。 [0004] Acid Orange II is a non-food coloring, food not allowed to join. 但是由于酸性橙II色泽鲜艳,着色稳定、价廉等特点,一些不法商贩为了牟取暴利将其用于食品生产和加工,我国市售和出口海外的蜜饯、豆制品、卤制品、辣椒面、红壳瓜子等均被检出过含有酸性橙II等类似合成非食用色素,严重危害了消费者身体健康,并且常常导致出口食品被迫就地销毁。 However, due to Acid Orange II bright color, coloring stable, inexpensive features, some unscrupulous traders to reap huge profits to be used for food production and processing, China's commercial and export overseas preserves, soy, halogen products, red pepper, red shell melon seeds were detected too contain similar synthesis of acid orange II and other non-food coloring, seriously endangering the health of consumers, and often results in the export of food forced destroyed on the spot. 所以酸性橙II 检测方法的开发刻不容缓。 Therefore, development of a method of detecting Acid Orange II delay.

[0005] 目前,检测食品中违法添加的酸性橙II的主要方法有高效液相色谱法及液质联用等进行定性和定量的分析。 [0005] Currently, the main method for detecting food Acid Orange II are added in the illegal HPLC and LC-MS and other qualitative and quantitative analysis. 这些方法对实验仪器设备要求高,操作繁琐,对操作人员的专业知识要求高,费时费力。 These methods are high requirements for laboratory equipment, complicated operation, high professional knowledge required for the operator, time-consuming and laborious. 因此建立一种准确可靠又快速方便的检测食品中违法添加的酸性橙II的分析方法是非常必要的。 Therefore, the establishment of a reliable and accurate analysis of food law to add acidic orange II of the quick and easy detection is necessary.

[0006] 本发明拟发明一种基于小分子物质酸性橙II偶联蛋白质后而具有抗原性的免疫学检测方法,采取免疫学竞争法的原理,结合胶体金或乳胶颗粒标记技术和免疫层析法设计的一种快速检测试剂,可快速特异地检测卤制熟食、辣椒面、饮料等样品中的酸性橙II。 [0006] The present invention is based on the latter invention is intended to small molecules Acid Orange II protein conjugation and immunological detection methods antigenic, immunological principles to take competition law, in conjunction with colloidal gold or latex particle-labeled immunochromatographic techniques and design a rapid method of detection reagent, the test sample can be cooked bittern, pepper, beverages and the like specifically acid orange II quickly. 根据检测卡样品检测线和质控线的有无显色来判定卤制熟食、辣椒面、饮料等样品中的酸性橙II含量。 The presence or absence of the color sample card detection test and control lines to determine the content of Acid Orange II samples bittern cooked, pepper, beverages and the like. 反应是利用酸性橙II蛋白质偶联物抗原性而建立的一种检测方法,具有特异性强的优点,可避免其它检测方法的假阳性导致的误检问题。 The reaction using Acid Orange II antigenic protein conjugate established a detection method has the advantage of a strong specificity, false detection can be avoided the problem of false positive results in other detection methods. 同时检测卡操作简单快速,无需特殊的仪器设备,既可以在实验室进行,也可在超市、家庭、车间等实地进行测定。 At the same time simple and rapid detection card operation, no special equipment, either in the laboratory, can also be measured on the ground in the supermarket, home, shop and so on. 由于样本量需要少、简便快速,适用于大量样本的筛查。 Since the sample size needed less, simple, rapid and suitable for screening large number of samples. 结果准确,灵敏度高,准确率大。 Accurate, high sensitivity, high accuracy rate.

发明内容 SUMMARY

[0007] 本发明的目的在于提供一种能检测酸性橙II的检测器具,直接应用免疫学的方法检测卤制熟食、辣椒面、饮料等样品中酸性橙II的含量,克服现有色谱法检测的操作繁琐等缺点,建立基于免疫学特异性的检测方法。 [0007] The object of the present invention is to provide a testing apparatus capable of detecting the Acid Orange II, direct application of immunological detection bittern cooked content of the sample, pepper, beverages and the like of Acid Orange II, to overcome the existing Chromatography complicated operation and other shortcomings, the establishment of specific immunological detection methods based.

[0008] 本发明的技术方案:一种酸性橙II快速检测卡,其为在长条扁平薄壳状的检测卡外壳1中设置测试条2,检测卡外壳表面有检测窗孔3和加样孔4 ;测试条2是由支承背板5上通过不干胶在其上依次粘贴样品垫8、胶体金膜或乳胶颗粒膜7、硝酸纤维素膜6、和吸水膜9所组成;支承背板中部叠置粘贴硝酸纤维素膜6,支承背板一端叠置粘贴吸水膜9,另一端叠置粘贴样品垫8,吸水膜内端与样品垫内端各自分别与硝酸纤维素膜搭接,在样品垫与硝酸纤维素膜的搭接部,两者之间夹置粘贴一段胶体金膜或乳胶颗粒膜7 ;胶体金膜为含抗酸性橙II抗体胶体金标记的玻璃纤维膜,或乳胶颗粒膜为含抗酸性橙II抗体乳胶颗粒标记的玻璃纤维膜,硝酸纤维素膜上有一条检测带和一条质控带,依次是:含酸性橙II蛋白质偶联物的检测带10,含抗兔抗体或抗鼠抗体的质控带11, [0008] The aspect of the present invention is: an acidic rapid detection card Orange II, No. 2, the card detection surface for detecting the aperture of the housing 3 and processing like a flat shell-shaped housing 1 is provided with a test card test strip in strip hole 4; back support; test strip 2 is supported on the back plate 5 on which the adhesive paste sequentially sample pad 8, latex particles or colloidal gold film 7 is a film, cellulose nitrate film 6, and the water-absorbing film 9 composed by stacked middle plate attached nitrocellulose membrane 6, the support end of the back plate attached absorbent laminated film 9, the other end of the sample pad 8 stacked adhesive, the inner end of the water-absorbing film and the end of the respective sample pad and the nitrocellulose membrane are overlapped, between the overlapping portion of the sample pad and the nitrocellulose membrane, both sandwiched copy some colloidal gold or latex particle film 7 film; colloidal gold-containing acid-resistant film is a glass fiber membrane orange II antibody labeled with colloidal gold or latex particle film containing antacid latex particle-labeled antibody II orange glass fiber membrane, a nitrocellulose membrane with a test line and a control line, are: acid orange II detecting protein-containing conjugate with 10, comprising an anti- QC rabbit anti-mouse antibody or antibody with 11, 试条置入检测卡外壳内,样品垫正对加样孔,硝酸纤维素膜正对检测窗孔。 Test strip into the test card within the housing, the loading aperture positive sample pad, a nitrocellulose membrane facing the detection aperture pair.

[0009] 采用上述酸性橙II快速检测卡,检测样品为卤制熟食、辣椒面或饮料等,处理方法如下: [0009] Acid Orange II above using the rapid test card, and other test samples for the bittern cooked food, beverage or pepper, processing as follows:

饮料样品:将购买的饮料如橙汁样品用PH为8. 0,含0. 05%吐温-20,浓度为0. lmol/L 的磷酸盐缓冲液(PBST)按1:1稀释,得到待测样品。 Beverage Sample: purchased beverage such as orange juice samples to PH 8.0, containing 0.05% Tween-20, at a concentration of 0. lmol / L phosphate buffer (PBST) by 1: 1 dilution, to be obtained test samples.

[0010] 卤制熟食、辣椒面样品:称取5g样品,研磨粉碎,加入上述pH8. 0、PBST溶液10mL, 振摇30min,膜过滤后用于测定。 [0010] bittern cooked, pepper samples: Weigh 5g sample, grind, added to the above pH8 0, PBST solution 10mL, shaken for 30min, after membrane filtration for measuring.

[0011] 当检测带10和质控带11同时显现棕红色印迹带,检测结果为阴性,表示被检测样品中酸性橙II含量未超标。 [0011] When the belt 10 is detected and the control line 11 simultaneously appear reddish brown blots with a detection result is negative, Acid Orange II represents the content of the test sample is not exceeded.

[0012] 当检测带10不显示颜色,只有质控带11显现棕红色印迹,检测结果为阳性,表示被检测样品中酸性橙II含量超标。 [0012] When the test strip 10 does not show color, only the control line 11 appear brownish red blot test result is positive, Acid Orange II represents an excessive content of the test sample.

[0013] 当检测带10和质控带11均不显色,则表明测试条已失效。 [0013] When the belt 10 is detected and the control line 11 are not color, it indicates that the test strip has expired.

[0014] 本发明的有益效果:本发明的优点是能检测卤制熟食、辣椒面、饮料等样品中含有的酸性橙II,易于制备且节省检测费用,使用方便,检测快速,灵敏度高,结果准确。 [0014] Advantageous effects of the invention: The advantages of the present invention is to accurately detect bittern cooked food, pepper, beverages Acid Orange II samples contained, easy to prepare and save testing costs, ease of use, detecting rapid, sensitive, .

附图说明 BRIEF DESCRIPTION

[0015] 图1酸性橙II快速检测卡外壳及测试条在外壳内的安置图。 [0015] FIG 1 Acid Orange II card case and rapid detection test strip disposed within the housing of FIG. 1、外壳,2、测试条,3、 检测窗孔,4、加样孔。 1, a housing 2, a test strip 3, the detection aperture, 4, sample hole.

[0016] 图2测试条的硝酸纤维素膜上显示印迹示意图。 [0016] The test strip of FIG. 2 displays a nitrocellulose membrane blotted FIG. 6、硝酸纤维素膜,10、检测带,11、 质控带。 6, a nitrocellulose membrane, 10, the detection zone 11, the control line.

[0017] 图3酸性橙II快速检测卡的检测试条结构示意图。 [0017] The schematic structure of the test strip of FIG. 3 detects rapid detection of Acid Orange II card. 5、支承背板,6、硝酸纤维素膜, 7、胶体金膜或乳胶颗粒膜,8、样品垫,9、吸水膜。 5, the support backplane, 6, nitrocellulose, 7, colloidal gold particles or latex film film 8, a sample pad, 9, water-absorbing film.

具体实施方式 detailed description

[0018] 本发明结合具体实施例参见附图进一步说明如下: 实施例1 [0018] Examples of the present invention with reference to specific embodiments further reference to the drawings as follows: Example 1

一种酸性橙II快速检测卡,它是在检测卡外壳1中设置测试条2,检测卡外壳1的结构及测试条2的设置参见附图1,检测卡外壳1为长条扁平薄壳状外壳,长70mm,宽20mm,厚5mm,薄壳壁厚1mm,由工程塑料制成。 Rapid detection of an acidic Orange II card, it is detecting the card 1 is provided in the housing 2 of the test strip, and a detection test strip card housing structure 1 provided 2 Refer to Figure 1, a test card shell-shaped housing 1 is a flat strip housing, length 70mm, width of 20mm, thickness of 5mm, shell wall thickness of 1mm, be made of plastic. 检测卡外壳1由壳盖与壳座两半联接构成,在检测卡外壳1壳盖上有检测窗孔3和加样孔4。 Test card 1 is constituted by a housing cover and the housing base shell halves coupled, detecting aperture cover 3 and processing 4 wells in a test card housing shell. 测试条2放置在检测卡外壳1的壳座内。 Test strip 2 is placed in the test card within the housing 1 of the housing base.

[0019] 测试条2是多层结构的窄条薄片,片长约60mm,片宽约4mm,厚度约2. 5mm。 [0019] The test strip 2 is narrow multilayer structure sheet, sheet of about 60mm, sheet width 4mm, a thickness of about 2. 5mm. 测试条2是多层结构:底层是支承背板5,为聚氯乙烯塑料薄片,厚约0. 5mm,长60mm,宽4mm ;支承背板5中部叠置粘贴硝酸纤维素膜6,硝酸纤维素膜厚0. 5mm,长20_25mm,宽4mm。 2 is a multilayer structure of the test strip: the underlying back sheet 5 is supported as a PVC sheet 0. 5mm, thickness, length 60mm, width 4mm; central support 5 superposed backsheet attached nitrocellulose membrane 6, nitrocellulose Su thickness of 0. 5mm, length 20_25mm, width of 4mm. 硝酸纤维素膜6上有二条间隔开的横向显示印迹带,参见附图2,二条显示印迹带中,一条是检测带10,含酸性橙II蛋白质偶联物;另一条是质控带11,含抗兔抗体或抗鼠抗体。 Nitrocellulose film 6 has two spaced apart transverse imprinted with a display, see figure 2, two blots show band, a belt 10 is detected, containing Acid Orange II protein conjugate; the other is the control line 11, containing anti-rabbit antibody or anti-mouse antibody. 支承背板5 一端部上叠置粘贴吸水膜9,支承背板5另一端部上叠置粘贴样品垫8,吸水膜9内端与样品垫8内端各自分别与硝酸纤维素膜6—端搭接,在样品垫8与硝酸纤维素膜6的搭接部, 两者之间夹置粘贴一段胶体金膜或乳胶颗粒膜7,胶体金膜为含抗酸性橙II抗体胶体金标记的玻璃纤维膜,或乳胶颗粒膜为含抗酸性橙II抗体乳胶颗粒标记的玻璃纤维膜。 Paste water-absorbing film laminated on the support portion 9 an end of the back plate 5, attached on the other end portion overlaps the back plate 5 is supported sample pad 8, 9 and the end of the water-absorbing films each end of the sample pad 8 respectively nitrocellulose end 6- lap, the lap portion 8 and the sample pad nitrocellulose film 6 sandwiched paste some colloidal gold or latex particle film membrane 7 therebetween, the colloidal gold-containing acid-resistant film is a glass orange II colloidal gold labeled antibody fiber membranes, latex particles, or a film containing the acid resistance of latex particle-labeled antibody II orange glass fiber membrane. 胶体金膜或乳胶颗粒膜7厚0. 5-0. 8mm,长5mm,宽4mm。 Latex particles or colloidal gold film 7 is a film thickness of 0. 5-0. 8mm, length 5mm, width 4mm. 样品垫8厚0. 5-0. 8mm,长20mm,宽4mm。 Thick sample pad 8 0. 5-0. 8mm, length 20mm, width of 4mm. 吸水膜9厚0. 5-0. 8mm,长16_20mm,宽4mm。 9 water-absorbing film thickness of 0. 5-0. 8mm, length 16_20mm, width of 4mm. 胶体金膜或乳胶颗粒膜7、样品垫8及吸水膜9与硝酸纤维素膜搭接长度为l_2mm。 Latex particles or colloidal gold film membrane 7, 8 and the water-absorbing film sample pad and the nitrocellulose membrane 9 lap length of l_2mm. 吸水膜9材料是吸水纸,样品垫8材料是吸水玻璃纤维膜。 Water-absorbing material is an absorbent paper membrane 9, the water-absorbing material is a sample pad 8 glass fiber membrane.

[0020] 本发明酸性橙II检测卡用于检测卤制熟食、辣椒面、饮料等样品中的酸性橙II的检测。 [0020] Acid Orange II test card of the present invention for detecting in a sample of Acid Orange bittern cooked, pepper, beverages detection II.

[0021] 实施例2 [0021] Example 2

样品的处理方法如下: Sample processing method is as follows:

饮料样品:将购买的饮料如橙汁样品用PH为8. 0,含0. 05%吐温-20,浓度为0. lmol/L 的磷酸盐缓冲液(PBST)按1:1稀释,得到待测样品。 Beverage Sample: purchased beverage such as orange juice samples to PH 8.0, containing 0.05% Tween-20, at a concentration of 0. lmol / L phosphate buffer (PBST) by 1: 1 dilution, to be obtained test samples.

[0022] 卤制熟食、辣椒面样品:称取5g样品,研磨粉碎,加入上述pH8. 0、PBST溶液10mL, 振摇30min,膜过滤后用于测定。 [0022] bittern cooked, pepper samples: Weigh 5g sample, grind, added to the above pH8 0, PBST solution 10mL, shaken for 30min, after membrane filtration for measuring.

[0023] 使用酸性橙II快速检测卡进行检测时,先将待检测的样品液从加样孔4中滴注入酸性橙II快速检测卡的样品垫8上,由于虹吸作用原理,带动待检测的样品液及胶体金膜或乳胶颗粒膜7所含的抗酸性橙II单克隆抗体胶体金或乳胶颗粒标记物一起向硝酸纤维素膜6扩散,5-10分钟内观察结果。 [0023] When using Acid Orange II Quick test card for testing, the sample solution to be detected from the first sample hole 4 drops of Acid Orange II samples rapid injection pad test card 8, since the principle of siphon action, led to be detected diffusion sample liquid and the latex particles or colloidal gold film 7 is a film of acid orange II monoclonal antibody contained in the latex particles or colloidal gold markers to a nitrocellulose membrane 6 together, observation over 5-10 minutes.

[0024] 检测卡的主要反应是免疫学的抗原和抗体反应,在硝酸纤维素膜上迁移的 The main reaction [0024] The test card is antigen and antibody immunological reaction, migration on a nitrocellulose membrane

胶体金或乳胶颗粒标记的抗体,在测试带上分别与含酸性橙II蛋白质偶联物,以及质控带抗兔抗体或抗鼠抗体反应,形成棕红色条带。 Latex particles or colloidal gold labeled antibody, respectively, in the test strip containing Acid Orange II protein conjugate, and a control line anti-rabbit antibody or anti-mouse antibody, to form a brownish red band. 若样品中有待检酸性橙II存在,当样品加入后即与抗体发生反应,而不会与检测带所含的酸性橙II蛋白质偶联物反应,从而不显色。 If the sample to be subject Acid Orange II is present, when added to the sample after the reaction with the antibody occurs without Acid Orange II protein conjugate contained in the reaction with the test strip, so that no color. 主要反应的结果有以下3种情况: The main result of the reaction of the following three conditions:

1、当检测带10和质控带11同时显现棕红色印迹带,表示检测结果为阴性,说明被检测样品中酸性橙II含量未超标。 1, when the test line 10 and control line 11 simultaneously visualized with red brown blots, indicating the detection result is negative, indicating Acid Orange II concentration is not exceeded in the test sample.

[0025] 2、当检测带10不显示颜色,只有质控带11显现棕红色印迹,检测结果为阳性,说明被检测样品中酸性橙II含量超标。 [0025] 2, when the test strip 10 does not show color, only the control line 11 appear brownish red blot test result is positive, indicating that the test sample is excessive levels of Acid Orange II.

[0026] 3、当检测带10和质控带11均不显色,则表明检测试条已失效。 [0026] 3, when not detecting the color band 10 and the control line 11, indicating the detection of the test strip has expired.

Claims (2)

  1. 1. 一种酸性橙II快速检测卡,其特征在于:在长条扁平薄壳状的检测卡外壳(1)中设置测试条(2),检测卡外壳表面有检测窗孔(3)和加样孔(4);测试条(2)是由支承背板(5) 上通过不干胶在其上依次粘贴样品垫(8)、胶体金膜或乳胶颗粒膜(7)、硝酸纤维素膜(6)、 和吸水膜(9)所组成;支承背板中部叠置粘贴硝酸纤维素膜(6),支承背板一端叠置粘贴吸水膜(9),另一端叠置粘贴样品垫(8),吸水膜内端与样品垫内端各自分别与硝酸纤维素膜搭接,在样品垫与硝酸纤维素膜的搭接部,两者之间夹置粘贴一段胶体金膜或乳胶颗粒膜(7);胶体金膜为含抗酸性橙II抗体胶体金标记的玻璃纤维膜,或乳胶颗粒膜为含抗酸性橙II抗体乳胶颗粒标记的玻璃纤维膜,硝酸纤维素膜上有一条检测带和一条质控带,依次是: 含酸性橙II蛋白质偶联物的检测带(10),含抗兔抗 Acid Orange II A rapid test card, wherein: the test card flat shell-shaped elongated housing (1) provided in the test strip (2), the detection surface for detecting the card housing aperture (3) and processing wells (4); a test strip (2) is a nitrocellulose membrane sequentially adhesive paste sample pad (8) on which is supported by the back plate (5) on the latex particles or colloidal gold film membrane (7), (6), and a water-absorbing film (9) consisting of; backsheet superposed central support attached nitrocellulose membrane (6), supporting one end of the back plate attached laminated water-absorbing film (9), the other end of the laminated paste sample pad (8 ), the inner end of the water-absorbing film and the end of the sample pad and the nitrocellulose membrane were each lap, the lap portion between the sample pad and the nitrocellulose membrane, both sandwiched copy some colloidal gold or latex particle film membrane ( 7); colloidal gold-containing acid-resistant film is a glass fiber membrane orange II antibody labeled colloidal gold, latex particles, or a film containing a glass fiber membrane antacid orange II antibody labeled latex particles, there is a nitrocellulose membrane and detected with a control line, are: acid orange II test strip containing the protein conjugate (10) containing anti-rabbit 或抗鼠抗体的质控带(11 ),测试条置入检测卡外壳内,样品垫正对加样孔,硝酸纤维素膜正对检测窗孔。 Or the control line (11) anti-mouse antibody, the test strip into the test card within the housing, the loading aperture positive sample pad, a nitrocellulose membrane facing the detection aperture pair.
  2. 2.用权利要求1所述的酸性橙II快速检测卡的检测方法,其特征是应用免疫学的方法直接检测样品中酸性橙II的含量,检测样品为卤制熟食、辣椒面或饮料,处理方法如下:饮料样品:将购买的饮料样品用PH8. 0、含0. 05%吐温-20的0. lmol/L的磷酸盐缓冲液PBST按1 : 1稀释,得到待测样品;卤制熟食、辣椒面样品:称取5g样品,研磨粉碎,加入所述pH 8. 0、PBST溶液IOmLjM 摇30min,膜过滤后用于测定;当检测带(10)和质控带(11)同时显现棕红色印迹带,检测结果为阴性,表示被检测样品中酸性橙II含量未超标;当检测带(10)不显示颜色,只有质控带(11)显现棕红色印迹,检测结果为阳性,表示被检测样品中酸性橙II含量超标;当检测带(10)和质控带(11)均不显色,则表明测试条已失效。 2. Acid Orange detection method as claimed in claim 1, said rapid detection II card, characterized in that the application of immunological methods directly detecting Acid Orange II content of the sample, the test sample is bittern cooked food, beverage or pepper, treatment . follows: beverage samples: sample drinks purchased by PH8 0, containing 0. lmol 0. 05% Tween-20 / L phosphate buffer PBST by 1: 1 dilution, to give test sample; bittern cooked food, pepper samples: Weigh 5g sample, grind, the added pH 8. 0, PBST solution IOmLjM shaking 30min, for measuring the membrane filtration; visualized simultaneously detected when the tape (10) and a control line (11) red brown imprinting belt, the detection result is negative, the test sample indicates the content is not exceeded acid orange II; when the detection zone (10) does not show color, only the control line (11) appear red brown blotting, the detection result is positive for the detection acid orange II samples content exceeded; when the detection zone (10) and a control line (11) is not a color, it indicates that the test strip has expired.
CN 201110141144 2011-05-30 2011-05-30 Quick detection card for acid orange II and detection method thereof CN102162814A (en)

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CN102798713A (en) * 2012-08-08 2012-11-28 广州市质量监督检测研究院 Detection kit for food red 10 and preparation method thereof
CN105301244A (en) * 2014-06-03 2016-02-03 北京勤邦生物技术有限公司 Enzyme linked immunosorbent assay kit for detecting acid orange and application of enzyme linked immunosorbent assay kit
CN106370847A (en) * 2016-08-22 2017-02-01 广州达元食品安全技术有限公司 Rapid detection kit of lemon yellow in food
CN106568965A (en) * 2016-10-14 2017-04-19 广州安诺食品科学技术有限公司 Auramine O colloidal gold test card and preparation method thereof
CN106645683A (en) * 2016-10-14 2017-05-10 广州安诺食品科学技术有限公司 Orange II colloidal gold testing card and preparation method thereof

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CN101915831A (en) * 2010-08-25 2010-12-15 南开大学 Enzyme-linked immunoassay kit for acid orange II
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WO2003006676A2 (en) * 2001-07-13 2003-01-23 Nanosphere, Inc. Method for immobilizing molecules onto surfaces
CN101625368A (en) * 2009-08-04 2010-01-13 无锡安迪生物工程有限公司 Casein rapid detection card and detection method thereof
CN101915831A (en) * 2010-08-25 2010-12-15 南开大学 Enzyme-linked immunoassay kit for acid orange II
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Publication number Priority date Publication date Assignee Title
CN102798713A (en) * 2012-08-08 2012-11-28 广州市质量监督检测研究院 Detection kit for food red 10 and preparation method thereof
CN102798713B (en) 2012-08-08 2014-08-27 广州市质量监督检测研究院 Detection kit for food red 10 and preparation method thereof
CN105301244A (en) * 2014-06-03 2016-02-03 北京勤邦生物技术有限公司 Enzyme linked immunosorbent assay kit for detecting acid orange and application of enzyme linked immunosorbent assay kit
CN106370847A (en) * 2016-08-22 2017-02-01 广州达元食品安全技术有限公司 Rapid detection kit of lemon yellow in food
CN106568965A (en) * 2016-10-14 2017-04-19 广州安诺食品科学技术有限公司 Auramine O colloidal gold test card and preparation method thereof
CN106645683A (en) * 2016-10-14 2017-05-10 广州安诺食品科学技术有限公司 Orange II colloidal gold testing card and preparation method thereof

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