CN201965133U - Fast BPA (bisphenol A) detection card - Google Patents
Fast BPA (bisphenol A) detection card Download PDFInfo
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- CN201965133U CN201965133U CN2011200581533U CN201120058153U CN201965133U CN 201965133 U CN201965133 U CN 201965133U CN 2011200581533 U CN2011200581533 U CN 2011200581533U CN 201120058153 U CN201120058153 U CN 201120058153U CN 201965133 U CN201965133 U CN 201965133U
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Abstract
A fast BPA (bisphenol A) detection card belongs to the field of detection technology for phenol content in polycarbonate finished products for a food container. The fast BPA (bisphenol A) detection card is characterized in that a test strip is arranged in a shell, and a sample pad, a colloidal gold membrane, a nitrocellulose membrane and a water absorption membrane are adhered on a supporting back plate in sequence; the colloidal gold membrane is a glass fiber membrane of an anti-BPA-containing monoclonal antibody or polyclonal antibody colloidal gold marker, two print display belts are arranged on the nitrocellulose membrane, one is a detection belt for a BPA-containing protein coupling, and the other one is a quality control belt containing a rabbit-resistant antibody or a mouse-resistant antibody. The fast BPA detection card has the advantages of directly detecting BPA content of a sample, such as a plastic milk bottle and the like, by applying an immunological method. The fast BPA detection car is easy for preparation, convenient and fast for use, and accurate for results.
Description
Technical field
The utility model relates to a kind of bisphenol-A rapid detection card, is a kind of appliance that detects bisphenol-A, belongs to food containers with phenol content detection technical field in the polycarbonate molding product.
Background technology
Bisphenol-A: being called for short bis-phenol base propane (BPA), is the important source material of polycarbonate, and many current consumption product such as articles for children such as food container, baby bottles all may contain bisphenol-A.Bisphenol-A belongs to environment incretion interferent, and in the middle of heating the time can precipitate into F﹠B, it may upset the body metabolism process, and is influential to infant development, immunity, even carcinogenic.In addition, bisphenol-A has female sex hormone effect, may cause feminism in boys, girl's sex premature etc.It influences adult physiological function simultaneously, and the workman reproductive function of long term exposure in bisphenol-A can be subjected to very big influence.
Because about the every result of study of bisphenol-A and the generation of adverse events, many countries all rectify the production and the use of this chemical substance.Taken the lead in forbidding such as, U.S. using the chemical substance bisphenol-A in the Food ﹠ Drink container such as baby bottles; Canadian government was just prohibited import and was sold the polycarbonate plastic baby bottles that contains the bisphenol-A composition last year.
European Union will forbid producing the plastics feeding bottle that contains bisphenol-A in March, 2011, sell the plastics feeding bottle that contains bisphenol-A from import prohibition in June, 2012 or in market.This measure causes the especially new mothers' of a lot of people worry, and the feeding bottle on the present home market is the PC material feeding bottle that contains bisphenol-A more than eighty per cant.
Regulation in " GB 14942-1994 food containers, wrappage with polycarbonate molding product hygienic standard ": aldehydes matter is not more than 0.05 milligram/kilogram by the stripping free amount that distilled water refluxed 6 hours in the feeding bottle.
At present, prior art has titrimetry, colourimetry and oscilloscopic polarography for the detection of bisphenol-A, and these method ubiquity complex operations require height to experimental instrument and equipment, need shortcomings such as technical skill personnel operation.
For this reason, the present invention sends out bright a kind of based on the antigenic immunological detection method of bisphenol-A protein conjugate, take immunology competition ratio juris, a kind of quick detection reagent of association colloid gold labelling technique and immunochromatographic method design can detect the bisphenol-A that contains in the plastics feeding bottle fast specifically.The content of bisphenol A of judging in the plastics feeding bottle sample that has or not according to test card sample test line (T) and control line (C).The present invention utilizes bisphenol-A protein conjugate antigenicity and a kind of detection method of setting up, has the advantage of high specificity, the flase drop problem that can avoid the false positive of other detection method to cause.The present invention since sample size need less, easy fast, be applicable to the examination of great amount of samples.
Summary of the invention
The purpose of this utility model is to provide a kind of appliance that can detect bisphenol-A, the method of applied immunology directly detects the content of bisphenol-A in the plastics feeding bottle, overcome the prior art complex operation, to the demanding shortcoming of instrument and equipment, set up based on the specific detection method of immunology.
The technical solution of the utility model: a kind of bisphenol-A rapid detection card, it is for being provided with test-strips 2 in the test card shell 1 of rectangular flat shell shape, and the test card case surface has the fenestra 3 of detection and well 4; Test-strips 2 is formed by pasting sample pad 8, collaurum film 7, nitrocellulose filter 6 and absorbing membrane 9 thereon successively by adhesive sticker on the supporting backboard 5; The stacked stickup nitrocellulose filter 6 in supporting backboard middle part, one endlap of supporting backboard is put and is pasted absorbing membrane 9, the stacked stickup sample pad 8 of the other end, absorbing membrane is inner to overlap with nitrocellulose filter respectively separately with sample pad the inner, at the clinch of sample pad and nitrocellulose filter, insert and put between the two and paste one section collaurum film 7; The collaurum film is the glass fibre membrane that contains anti-bisphenol A antibody colloidal gold mark, there is one to detect band and a quality control band on the nitrocellulose filter, be successively: the detection that contains the bisphenol-A protein conjugate is with 10, the quality control band 11 that contains anti-rabbit antibody or anti-mouse antibody, test-strips is inserted in the test card shell, sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
Adopt above-mentioned bisphenol-A rapid detection card, test sample can be food containers such as plastics feeding bottle, water bottle.
The disposal route of its test sample is: takes by weighing about 1g sample, is accurate to 0.01g, and adding distil water 100mL, little the boiling of water-bath heating refluxed 6 hours, got distillate and was used for measuring.
When detection be with 10 and quality control band 11 manifest brownish red trace band simultaneously, testing result is negative, represents that content of bisphenol A is lower than permissible value in the detected sample;
Be with 10 Show Colors not when detection, have only quality control band 11 to manifest the brownish red trace, testing result is positive, represents that content of bisphenol A is higher than permissible value in the detected sample;
When detection be with 10 and quality control band 11 all do not develop the color, show that then test-strips lost efficacy.
The utility model has the advantages that and can detect the bisphenol-A that contains in the plastics feeding bottle, be easy to preparation and save testing cost, easy to use, detect fast, highly sensitive, the result is accurate.
Description of drawings
Fig. 1 bisphenol-A rapid detection card shell and test-strips arrangement figure in the enclosure.1, test card shell, 2, test-strips, 3, detect fenestra, 4, well.
Show the trace synoptic diagram on the nitrocellulose membrane of Fig. 2 test-strips.6, nitrocellulose filter, 10, detect band, 11, quality control band.
The test-strips structural representation of Fig. 3 bisphenol-A rapid detection card.5, supporting backboard, 6, nitrocellulose filter, 7, the collaurum film, 8, sample pad, 9, absorbing membrane.
Embodiment
The utility model further specifies as follows in conjunction with specific embodiments referring to accompanying drawing:
A kind of bisphenol-A rapid detection card, it is that test-strips 2 is set in test card shell 1, the setting of the structure of test card shell 1 and test-strips 2 is referring to accompanying drawing 1, test card shell 1 is rectangular flat shell shape shell, long 70mm, wide 20mm, thick 5mm, shell wall thickness 1mm is made by engineering plastics.Test card shell 1 is connected with the shell block two halves by cap and constitutes, and the fenestra 3 of detection and well 4 are arranged on test card shell 1 cap.Test-strips 2 is placed in the shell block of test card shell 1.
Test-strips 2 is fillet thin slices of sandwich construction, and sheet is about 60mm, the wide about 4mm of sheet, the about 2.5mm of thickness.Test-strips 2 is sandwich constructions: bottom is a supporting backboard 5, is the igelite thin slice, thick about 0.5mm, long 60mm, wide 4mm; The stacked stickup nitrocellulose filter 6 in supporting backboard 5 middle parts, cellulose nitrate thickness 0.5mm, long 20-25mm, wide 4mm.Two isolated horizontal demonstration trace bands are arranged on the nitrocellulose filter 6, show in the trace band referring to 2, two in accompanying drawing, one is to detect to be with 10, contains the bisphenol-A protein conjugate; Another is a quality control band 11, contains anti-rabbit antibody or anti-mouse antibody.Stacked stickup absorbing membrane 9 on supporting backboard 5 one ends, stacked stickup sample pad 8 on supporting backboard 5 the other end, absorbing membrane 9 the inners overlap with nitrocellulose filter 6 one ends respectively separately with sample pad 8 the inners, at the clinch of sample pad 8 with nitrocellulose filter 6, insert and put between the two and paste one section collaurum film 7, collaurum film 7 is the glass fibre membranes that contain anti-bisphenol A monoclonal antibody or polyclonal antibody colloid gold label thing.Collaurum film 7 thick 0.5-0.8mm, long 5mm, wide 4mm.Sample pad 8 thick 0.5-0.8mm, long 20mm, wide 4mm.Absorbing membrane 9 thick 0.5-0.8mm, long 16-20mm, wide 4mm.Collaurum film 7, sample pad 8 and absorbing membrane 9 are 1-2mm with the nitrocellulose filter lap of splice.Absorbing membrane 9 materials are thieving paper, and sample pad 8 materials are water adsorption glass tunica fibrosas.
The utility model bisphenol-A test card is used for the detection of the content of bisphenol A of plastics feeding bottle.
Embodiment 2
The disposal route of sample is:
Take by weighing about 1g sample, be accurate to 0.01g, adding distil water 100mL, little the boiling of water-bath heating refluxed 6 hours, got distillate and was used for measuring.
When using the bisphenol-A rapid detection card to detect, earlier sample liquid to be detected is instilled into from well 4 on the sample pad 8 of bisphenol-A rapid detection card, because syphonic effect principle, drive contained anti-bisphenol A monoclonal antibody of sample liquid to be detected and collaurum film 7 or polyclonal antibody colloid gold label things together to nitrocellulose filter 6 diffusions, observations in 5-10 minute.
The key reaction of test card is immunologic antigen and antibody response, the antibody of the colloid gold label that on nitrocellulose filter, moves, on calibration tape respectively with contain the bisphenol-A protein conjugate, and the anti-rabbit antibody of quality control band or anti-mouse antibody reaction forms the brownish red band.If there is in the sample bisphenol-A to be checked to exist, after sample adds, promptly react with antibody, and not can with detection with contained bisphenol-a reaction, thereby do not develop the color.The result of key reaction has following 3 kinds of situations:
1, when show to detect be with 10 and quality control band 11 manifest brownish red trace band simultaneously, the expression testing result is negative, illustrates that content of bisphenol A does not exceed standard in the detected sample;
2, be with 10 Show Colors not when detection, have only quality control band 11 to manifest the brownish red trace, testing result is positive, illustrates that content of bisphenol A exceeds standard in the detected sample;
3, when detection be with 10 and quality control band 12 all do not develop the color, show that then detecting strip lost efficacy.
Claims (1)
1. bisphenol-A rapid detection card, it is characterized in that: in the test card shell (1) of rectangular flat shell shape test-strips (2) is set, the test card case surface has detection fenestra (3) and well (4); Test-strips (2) is upward pasted sample pad (8), collaurum film (7), nitrocellulose filter (6) and absorbing membrane (9) thereon successively by adhesive sticker by supporting backboard (5) and is formed; Supporting backboard middle part stacked stickup nitrocellulose filter (6), one endlap of supporting backboard is put and is pasted absorbing membrane (9), the stacked stickup sample pad of the other end (8), absorbing membrane is inner to overlap with nitrocellulose filter respectively separately with sample pad the inner, at the clinch of sample pad and nitrocellulose filter, insert and put between the two and paste one section collaurum film (7); The collaurum film is the glass fibre membrane that contains anti-bisphenol A antibody colloidal gold mark, there is one to detect band and a quality control band on the nitrocellulose filter, be successively: the detection band (10) that contains the bisphenol-A protein conjugate, the quality control band (11) that contains anti-rabbit antibody or anti-mouse antibody, test-strips is inserted in the test card shell, sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011200581533U CN201965133U (en) | 2011-03-08 | 2011-03-08 | Fast BPA (bisphenol A) detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011200581533U CN201965133U (en) | 2011-03-08 | 2011-03-08 | Fast BPA (bisphenol A) detection card |
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| Publication Number | Publication Date |
|---|---|
| CN201965133U true CN201965133U (en) | 2011-09-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2011200581533U Expired - Fee Related CN201965133U (en) | 2011-03-08 | 2011-03-08 | Fast BPA (bisphenol A) detection card |
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| CN (1) | CN201965133U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507929A (en) * | 2011-10-28 | 2012-06-20 | 合肥工业大学 | Signal-enhancement type immunochromatographic gold-labeled test strip and preparation method thereof |
| CN103018467A (en) * | 2012-09-27 | 2013-04-03 | 江苏维赛科技生物发展有限公司 | Colloidal gold immunochromatographic card for detecting bisphenol A (BPA) and preparation method thereof |
| CN108845131A (en) * | 2018-06-20 | 2018-11-20 | 广州质量监督检测研究院 | Detect the colloidal gold immunochromatographimethod detection card and its preparation method and application of bisphenol b |
-
2011
- 2011-03-08 CN CN2011200581533U patent/CN201965133U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507929A (en) * | 2011-10-28 | 2012-06-20 | 合肥工业大学 | Signal-enhancement type immunochromatographic gold-labeled test strip and preparation method thereof |
| CN102507929B (en) * | 2011-10-28 | 2014-03-26 | 合肥工业大学 | Preparation method of signal-enhancement type immunochromatographic gold-labeled test strip |
| CN103018467A (en) * | 2012-09-27 | 2013-04-03 | 江苏维赛科技生物发展有限公司 | Colloidal gold immunochromatographic card for detecting bisphenol A (BPA) and preparation method thereof |
| CN108845131A (en) * | 2018-06-20 | 2018-11-20 | 广州质量监督检测研究院 | Detect the colloidal gold immunochromatographimethod detection card and its preparation method and application of bisphenol b |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110907 Termination date: 20180308 |
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| CF01 | Termination of patent right due to non-payment of annual fee |