CN102507929B - Preparation method of signal-enhancement type immunochromatographic gold-labeled test strip - Google Patents
Preparation method of signal-enhancement type immunochromatographic gold-labeled test strip Download PDFInfo
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Abstract
The invention discloses a signal-enhancement type immunochromatographic gold-labeled test strip and a preparation method thereof. The preparation method of the gold-labeled test strip comprises the following steps of: (1) combining colloidal gold nanometer particles with different particle diameters; (2) preparing a colloidal gold immunity detection probe; (3) preparing a colloidal gold signal enhancement probe; (4) mounting signal-enhancement type test paper; and (5) establishing a detection method of the signal-enhancement type test paper. The signal-enhancement type immunochromatographic gold-labeled test strip, disclosed by the invention, can be applied to field fast ultra-sensitive detection without needing other auxiliary instruments, and has the following advantages that: a detection operation can be finished within 10-15 minutes and a detection result can be obtained; the sensitivity of the traditional test paper detection method can be remarkably improved, and the ultra-sensitive detection of a low-concentration appointed target object which is not realized by the traditional test paper is realized; and as long as an antibody of the immunity identification probe of the test paper is correspondingly replaced, the detection of other target objects can be realized; the specificity is high, the stability is good, the application range is wide, the cost is low and the like; in addition, the signal-enhancement type immunochromatographic gold-labeled test strip and the preparation method thereof, disclosed by the invention, are easy to promote and apply.
Description
Technical field
The invention belongs to the gold label test strip technical field that food security, inspection and quarantine, environment measuring and disease early diagnosis are used, be specifically related to a kind of signal enhancement mode hypersensitive Gold-immunochromatography assay test strips and preparation method thereof.
Background technology
Along with the increase of social development and international foreign trade amount, the Detection task that inspection and quarantine and food safety detection face is more and more heavier, and magnanimity detects sample size inspection and quarantine and food safety detection have been proposed to new demand.It is high that tradition relies on the detection method of instrument to have detection sensitivity, the advantages such as testing result good stability.But because large-scale instrument has expensively, operating personnel must have relevant professional knowledge, and the shortcoming such as testing cost costliness has determined to rely on the detection method of large-scale instrument in various basic units and Site Detection, extensively to promote the use of.Detection method based on immuno analytical method has been alleviated the severe situation that current detection faces to a certain extent.As enzyme linked immunosorbent assay analysis method, there is detection sensitivity high, the feature such as testing cost is relatively cheap.The appearance of colloidal gold colloidal gold detection test paper strip is for there being great innovation on detection time, the fast detecting of realize target thing in being less than the time range of 1 hour.Meanwhile, because gold label test strip detection technique does not need other complicated instruments in testing process, only need can judge the testing result of test strips by visual inspection.Under such condition, the detection sensitivity of test strips is significant for the judgement of testing result.But gold label test strip is developed so far, the optimization of its detection sensitivity is one of research emphasis of test strips related detecting method always.
Summary of the invention
The sensitivity that the present invention is directed to existing gold label test strip and related detecting method thereof needs the present Research further improving, utilize the specific surface effect of nano material, carry out the research of gold label test strip detection signal enhancing, a kind of signal enhancement mode immunochromatographigold gold-labeled test strip and preparation method thereof is provided, has realized target detection thing is carried out to hypersensitive field quick detection.
The concrete technical scheme of the present invention is as follows:
A kind of signal enhancement mode hypersensitive immunochromatographigold gold-labeled test strip comprises plastics lining board, on described plastics lining board, be pasted with successively respectively mother glass tunica fibrosa, immunological probe gold mark pad, amplification of signal gold mark pad, five kinds of adhesive matter of nitrocellulose membrane and water adsorption glass tunica fibrosa; Between adjacent adhesive matter, the length of lap is 2 mm, and dry encapsulation, preserves under 4 ℃～normal temperature condition of temperature.
A kind of preparation method's of signal enhancement mode immunochromatographigold gold-labeled test strip concrete preparation manipulation step is as follows:
(1) preparation of target detection thing gold-marking immunity probe
Choosing the colloidal gold solution that particle diameter is 30～40nm, is the sal tartari (K of 0.1 M by concentration
2cO
3) solution regulates pH value to 8.5～9.5 of colloidal gold solution, must regulate colloidal gold solution A afterwards;
Bovine serum albumin(BSA) (BSA) solution that the anti-target detection thing of the rabbit antibody-solutions that by concentration is 1mg～2 mg/mL is 1 mg～2 mg/mL with concentration mixes with mol ratio 1:100, obtains mixed solution;
Get mixed solution 10 μ L and join in the rear colloidal gold solution A of 1 mL adjusting, oscillating reactions 15min under room temperature;
Under 4 ℃ of conditions of temperature, with centrifugal 20 min of speed of 10000 rpm, remove the unconjugated antibody of supernatant and bovine serum albumin(BSA) (BSA);
The centrifugal precipitation obtaining is scattered in phosphate buffer solution (PBS) again, obtains target detection thing gold-marking immunity probe; (2) preparation of gold mark amplification of signal probe
Choosing the colloidal gold solution that particle diameter is 15～20 nm, is the sal tartari (K of 0.1 M by concentration
2cO
3) solution regulates pH value to 8.5～9.5 of colloidal gold solution, must regulate colloidal gold solution B afterwards;
After joining and regulate, bovine serum albumin(BSA) (BSA) antibody-solutions that the mark ratio of 1:1000～1:3000 of take is 1mg/mL by concentration in colloidal gold solution B, under room temperature, reacts 15min; Under 4 ℃ of conditions of temperature, after centrifugal 20 min of speed with 10000 rpm, remove unconjugated bovine serum albumin(BSA) (BSA) antibody in supernatant;
The centrifugal precipitation obtaining is scattered in phosphate buffer solution (PBS) again, obtains gold mark amplification of signal probe;
(3) preparation of target detection thing immunological probe gold mark pad and target detection thing amplification of signal gold mark pad
Target detection thing gold-marking immunity probe and gold mark amplification of signal probe are sprayed to respectively on two glass fibre membranes, obtain target detection thing immunological probe gold mark pad and target detection thing amplification of signal gold mark pad;
(4) there is the preparation of the nitrocellulose membrane of detection line and line of reference
The goat anti-rabbit antibody solution that the target detection thing-ovalbumin conjugate solution that is 1mg/mL by concentration on a nitrocellulose membrane and concentration are 1mg/mL is drawn respectively the line of two line parallels, the line of target detection thing-ovalbumin conjugate is as detection line, and goat anti-rabbit antibody solution is scribed ss line of reference;
(5) preparation of signal enhancement mode immunochromatographigold gold-labeled test strip
Nitrocellulose membrane and five kinds of adhesive matter of water adsorption glass tunica fibrosa of mother glass tunica fibrosa, the golden mark pad of target detection thing immunological probe, target detection thing amplification of signal gold mark being padded, have to detection line and line of reference paste on plastics lining board respectively successively; The length of the lap between adjacent adhesive matter is 2 mm, obtains signal enhancement mode super sensitivity detection gold label test strip, and dry encapsulation, preserves under 4 ℃～normal temperature condition of temperature.
The anti-target detection thing of rabbit antibody in described step 1 is rabbit anti-bisphenol A antibody, the anti-phthalic ester antibody of rabbit or the anti-Lay of rabbit cordoba amine antibody.
Useful technique effect of the present invention embodies in the following areas:
1, the present invention is applied to large this distinguishing feature of nano particle specific surface area foundation and the corresponding detection method of traditional gold label test strip, set up and can be used for the signal enhancement mode colloidal gold mark test paper of on-the-spot fast super sensitivity detection and relevant detection method, by naked eyes, directly observed that fast qualitative that realize target detects thing detects and reduction by the degree that develops the color on test strip quantitatively detects target detection thing.Without other supplementary instrument equipment, testing can complete and obtain testing result in 10-15 minute;
2, the present invention the most the part of core for to increase powerful design by signal, can significantly improve the sensitivity of traditional ELISA test strip method, realize the super sensitivity detection of the low concentration specific objective thing that traditional test strips cannot realize;
3, the present invention is universal immunochromatography colloidal gold mark test paper, only immunity identification probe antibody used in test strips need be carried out to corresponding replacement, can realize the detection of other objects;
4, also to have specificity high in the present invention, good stability, and applied range, with low cost, operation using method is simply easy to apply.Port such as customs, airport that can be applicable to hospital, family and food security and inspection and quarantine etc. needs field quick detection mechanism.
Accompanying drawing explanation
Fig. 1 is the side structure schematic diagram of signal enhancement mode gold label test strip.
Sequence number in Fig. 1: mother glass tunica fibrosa 1, immunological probe gold mark pad 2, amplification of signal gold are marked pad 3, had nitrocellulose membrane 4, water adsorption glass tunica fibrosa 5, plastics lining board 10, detection line 11, the line of reference 12 of detection line and line of reference.
Fig. 2 is the positive testing result schematic diagram of signal enhancement mode gold label test strip.
Embodiment
Below in conjunction with embodiment, the present invention is further described.
A kind of preparation method's concrete operation step of signal enhancement mode bisphenol-A immunochromatographigold gold-labeled test strip is as follows:
(1) preparation of rabbit anti-bisphenol A gold-marking immunity probe
Choosing the colloidal gold solution that particle diameter is 40nm, is the sal tartari (K of 0.1 M by concentration
2cO
3) solution regulates the pH value to 8.5 of colloidal gold solution, must regulate colloidal gold solution A afterwards;
Bovine serum albumin(BSA) (BSA) solution that the rabbit anti-bisphenol A antibody-solutions that is 1mg/mL by concentration is 1 mg/mL with concentration mixes with mol ratio 1:100, obtains mixed solution;
Get mixed solution 10 μ L and join in the rear colloidal gold solution A of 1 mL adjusting, oscillating reactions 15min under room temperature;
Under 4 ℃ of conditions of temperature, with centrifugal 20 min of speed of 10000 rpm, remove the unconjugated antibody of supernatant and bovine serum albumin(BSA) (BSA);
The centrifugal precipitation obtaining is scattered in phosphate buffer solution (PBS) again, obtains rabbit anti-bisphenol A gold-marking immunity probe; (2) preparation of rabbit anti-bisphenol A gold mark amplification of signal probe
Choosing the colloidal gold solution that particle diameter is 20 nm, is the sal tartari (K of 0.1 M by concentration
2cO
3) solution regulates the pH value to 8.5 of colloidal gold solution, must regulate colloidal gold solution B afterwards;
After joining and regulate, bovine serum albumin(BSA) (BSA) antibody-solutions that the mark ratio of 1:1000 of take is 1mg/mL by concentration in colloidal gold solution B, under room temperature, reacts 15min; Under 4 ℃ of conditions of temperature, after centrifugal 20 min of speed with 10000 rpm, remove unconjugated bovine serum albumin(BSA) (BSA) antibody in supernatant;
The centrifugal precipitation obtaining is scattered in phosphate buffer solution (PBS) again, obtains rabbit anti-bisphenol A gold mark amplification of signal probe;
(3) preparation of rabbit anti-bisphenol A immunological probe gold mark pad and rabbit anti-bisphenol A amplification of signal gold mark pad
Rabbit anti-bisphenol A gold-marking immunity probe and rabbit anti-bisphenol A gold mark amplification of signal probe are sprayed to respectively on two glass fibre membranes, obtain rabbit anti-bisphenol A immunological probe gold mark pad and rabbit anti-bisphenol A amplification of signal gold mark pad;
(4) there is the preparation of the nitrocellulose membrane of detection line and line of reference
The goat anti-rabbit antibody solution that the bisphenol-A-ovalbumin conjugate solution that is 1mg/mL by concentration on a nitrocellulose membrane and concentration are 1mg/mL is drawn respectively the line of two line parallels, the line of rabbit anti-bisphenol A-ovalbumin conjugate is as detection line, and goat anti-rabbit antibody solution is scribed ss line of reference;
(5) preparation of signal enhancement mode bisphenol-A immunochromatographigold gold-labeled test strip
Referring to Fig. 1, on plastics lining board 10, paste successively respectively mother glass tunica fibrosa 1, rabbit anti-bisphenol A immunological probe gold mark pad 2, golden nitrocellulose membrane 4 and the water adsorption glass tunica fibrosa 5 of marking pad 3, thering is detection line and line of reference of rabbit anti-bisphenol A amplification of signal; The length of the lap between adjacent adhesive matter is 2 mm, obtains signal enhancement mode bisphenol-A super sensitivity detection gold label test strip, and dry encapsulation, preserves under 4 ℃～normal temperature condition of temperature.
During use, the mother glass tunica fibrosa end of signal enhancement mode rabbit anti-bisphenol A gold label test strip is inserted in solution to be detected, after wait 10 min, takes out the testing result that detects by an unaided eye.If developing the color simultaneously, the test strip on film and contrast band show that testing result is effective; Compare with the test strip of blank group, if the test strip color of test set shoals, show to contain object rabbit anti-bisphenol A in solution to be detected, in the more shallow detection solution that develops the color, rabbit anti-bisphenol A concentration is higher, positive result; As only have test strip colour developing and contrast band does not develop the color, show that testing result is invalid.
embodiment 2:
A kind of preparation method's concrete operation step of signal enhancement mode phthalic ester immunochromatographigold gold-labeled test strip is as follows:
(1) preparation of the anti-phthalic ester gold-marking immunity of rabbit probe
Choose the colloidal gold solution that particle diameter is 30nm, with sal tartari (K2CO3) solution that concentration is 0.1 M, regulate the pH value to 9.5 of colloidal gold solution, colloidal gold solution A after must regulating;
Bovine serum albumin(BSA) (BSA) solution that the anti-phthalic ester antibody-solutions of the rabbit that is 2mg/mL by concentration is 2mg/mL with concentration mixes with mol ratio 1:100, obtains mixed solution;
Get mixed solution 10 μ L and join in the rear colloidal gold solution A of 1 mL adjusting, oscillating reactions 15min under room temperature;
Under 4 ℃ of conditions of temperature, with centrifugal 20 min of speed of 10000 rpm, remove the unconjugated antibody of supernatant and bovine serum albumin(BSA) (BSA);
The centrifugal precipitation obtaining is scattered in phosphate buffer solution (PBS) again, obtains the anti-phthalic ester gold-marking immunity of rabbit probe;
(2) preparation of the anti-phthalic ester gold of rabbit mark amplification of signal probe
Choose the colloidal gold solution that particle diameter is 15nm, with sal tartari (K2CO3) solution that concentration is 0.1 M, regulate the pH value to 9.5 of colloidal gold solution, colloidal gold solution B after must regulating;
After joining and regulate, bovine serum albumin(BSA) (BSA) antibody-solutions that the mark ratio of 1:3000 of take is 1mg/mL by concentration in colloidal gold solution B, under room temperature, reacts 15min; Under 4 ℃ of conditions of temperature, after centrifugal 20 min of speed with 10000 rpm, remove unconjugated bovine serum albumin(BSA) (BSA) antibody in supernatant;
The centrifugal precipitation obtaining is scattered in phosphate buffer solution (PBS) again, obtains the anti-phthalic ester gold of rabbit mark amplification of signal probe;
(3) preparation of the anti-phthalic ester immunological probe gold of rabbit mark pad and the anti-phthalic ester amplification of signal gold of rabbit mark pad
The anti-phthalic ester immunological probe of rabbit and the anti-phthalic ester amplification of signal of rabbit probe are sprayed to respectively on two glass fibre membranes, obtain respectively the anti-phthalic ester immunological probe gold of rabbit mark pad and the anti-phthalic ester amplification of signal gold of rabbit mark pad;
(4) there is the preparation of the nitrocellulose membrane of detection line and line of reference
On a nitrocellulose membrane, by concentration, be the line that the anti-phthalic ester-ovalbumin of the rabbit conjugate solution of 0.5 mg/mL and goat anti-rabbit antibody solution that concentration is 0.5 mg/mL are drawn respectively two line parallels, the line of the anti-phthalic ester-ovalbumin of rabbit conjugate is as detection line, and goat anti-rabbit antibody solution is scribed ss line of reference;
(5) preparation of the anti-phthalic ester immunochromatographigold gold-labeled test strip of signal enhancement mode rabbit
Referring to Fig. 1, on plastics lining board 1, paste successively respectively mother glass tunica fibrosa 2, the anti-phthalic ester immunological probe gold of rabbit mark pad 3, golden nitrocellulose membrane 5 and the water adsorption glass tunica fibrosa 6 of marking pad 4, thering is detection line and line of reference of the anti-phthalic ester amplification of signal of rabbit; The length of the lap between adjacent adhesive matter is 2 mm, obtains signal enhancement mode phthalic ester super sensitivity detection gold label test strip, and dry encapsulation, preserves under 4 ℃～normal temperature condition of temperature.
Preparation method's concrete operation step of the anti-Lay of a kind of signal enhancement mode rabbit cordoba amine immunochromatographigold gold-labeled test strip is as follows:
(1) preparation of the anti-Lay of rabbit cordoba amine gold-marking immunity probe
Choosing the colloidal gold solution that particle diameter is 30nm, is the sal tartari (K of 0.1 M by concentration
2cO
3) solution regulates the pH value to 9.5 of colloidal gold solution, must regulate colloidal gold solution A afterwards;
Bovine serum albumin(BSA) (BSA) solution that the anti-Lay of the rabbit cordoba amine antibody-solutions that is 2mg/mL by concentration is 2mg/mL with concentration mixes with mol ratio 1:100, obtains mixed solution;
Get mixed solution 10 μ L and join in the rear colloidal gold solution A of 1 mL adjusting, oscillating reactions 15min under room temperature;
Under 4 ℃ of conditions of temperature, with centrifugal 20 min of speed of 10000 rpm, remove the unconjugated antibody of supernatant and bovine serum albumin(BSA) (BSA);
The centrifugal precipitation obtaining is scattered in phosphate buffer solution (PBS) again, obtains the anti-Lay of rabbit cordoba amine gold-marking immunity probe;
(2) preparation of the anti-Lay of rabbit cordoba amine gold mark amplification of signal probe
Choosing the colloidal gold solution that particle diameter is 15nm, is the sal tartari (K of 0.1 M by concentration
2cO
3) solution regulates the pH value to 9.5 of colloidal gold solution, must regulate colloidal gold solution B afterwards;
After joining and regulate, bovine serum albumin(BSA) (BSA) antibody-solutions that the mark ratio of 1:3000 of take is 1mg/mL by concentration in colloidal gold solution B, under room temperature, reacts 15min; Under 4 ℃ of conditions of temperature, after centrifugal 20 min of speed with 10000 rpm, remove unconjugated bovine serum albumin(BSA) (BSA) antibody in supernatant;
The centrifugal precipitation obtaining is scattered in phosphate buffer solution (PBS) again, obtains the anti-Lay of rabbit cordoba amine gold mark amplification of signal probe;
(3) preparation of the anti-Lay of rabbit cordoba amine immunological probe gold mark pad and the anti-Lay of rabbit cordoba amine amplification of signal gold mark pad
The anti-Lay of rabbit cordoba amine immunological probe and the anti-Lay of rabbit cordoba amine amplification of signal probe are sprayed to respectively on two glass fibre membranes, obtain respectively the anti-Lay of rabbit cordoba amine immunological probe gold mark pad and the anti-Lay of rabbit cordoba amine amplification of signal gold mark pad;
(4) there is the preparation of the nitrocellulose membrane of detection line and line of reference
On a nitrocellulose membrane, by concentration, be the line that the anti-Lay of rabbit cordoba amine-ovalbumin conjugate solution of 0.5 mg/mL and goat anti-rabbit antibody solution that concentration is 0.5 mg/mL are drawn respectively two line parallels, amine-ovalbumin conjugate the line of the anti-Lay of rabbit cordoba is as detection line, and goat anti-rabbit antibody solution is scribed ss line of reference;
(5) preparation of the anti-Lay of signal enhancement mode rabbit cordoba amine immunochromatographigold gold-labeled test strip
Referring to Fig. 1, on plastics lining board 1, paste successively respectively mother glass tunica fibrosa 2, the anti-Lay of rabbit cordoba amine immunological probe gold mark pad 3, golden nitrocellulose membrane 5 and the water adsorption glass tunica fibrosa 6 of marking pad 4, thering is detection line and line of reference of the anti-Lay of rabbit cordoba amine amplification of signal; The length of the lap between adjacent adhesive matter is 2 mm, obtains the anti-Lay of signal enhancement mode rabbit cordoba amine super sensitivity detection gold label test strip, and dry encapsulation, preserves under 4 ℃～normal temperature condition of temperature.
Embodiment 4:
Referring to Fig. 2, wherein sequence number 2 is mother glass tunica fibrosa, and length is 18mm; Sequence number 3 is gold-marking immunity identification probe pads, and length is 4 mm; Sequence number 4 is gold mark amplification of signal probe pads, and length is 4 mm; Sequence number 5 is nitrocellulose membrane, and length is 20 mm; Sequence number 6 is adsorptive pads, and length is 18 mm, and sequence number 11 is detection line, and width is 1mm, and sequence number 12 is line of reference, and width is 1 mm.Arrow express liquid capillary action direction in figure.
Fig. 2 is for utilizing signal enhancement colloidal gold mark test paper to carry out in object testing process, the basis for estimation of testing result.How the testing result by signal enhancement mode gold label test strip judges detecting the content of object in sample, is the important component part of detection method.Concrete testing result basis for estimation is as follows:
In Fig. 2, the first from left is the negative result schematic diagram of signal enhancement mode gold label test strip,
In Fig. 2, the second from left is the weak positive test symbol schematic diagram of signal enhancement mode gold label test strip,
In Fig. 2, right one is the positive test symbol schematic diagram of signal enhancement mode gold label test strip,
In Fig. 2, right two is invalid signal enhancement metal mark ELISA test strip result schematic diagram.
Claims (1)
1. a preparation method for signal enhancement mode immunochromatographigold gold-labeled test strip, is characterized in that concrete preparation manipulation step is as follows:
(1) preparation of target detection thing gold-marking immunity probe
Choose the colloidal gold solution that particle diameter is 30～40nm, the sal tartari (K that is 0.1M by concentration
2cO
3) solution regulates pH value to 8.5～9.5 of colloidal gold solution, must regulate colloidal gold solution A afterwards;
Bovine serum albumin(BSA) (BSA) solution that the anti-target detection thing of the rabbit antibody-solutions that is 1～2mg/mL by concentration is 1～2mg/mL with concentration mixes with mol ratio 1:100, obtains mixed solution; Wherein the anti-target detection thing of rabbit antibody is rabbit anti-bisphenol A antibody, the anti-phthalic ester antibody of rabbit or the anti-Anti-ractopamine antibody of rabbit;
Get mixed solution 10 μ L and join in the rear colloidal gold solution A of 1mL adjusting, oscillating reactions 15min under room temperature;
Under 4 ℃ of conditions of temperature, with the centrifugal 20min of speed of 10000rpm, remove the unconjugated antibody of supernatant and bovine serum albumin(BSA) (BSA);
The centrifugal precipitation obtaining is scattered in phosphate buffer solution (PBS) again, obtains target detection thing gold-marking immunity probe;
(2) preparation of gold mark amplification of signal probe
Choose the colloidal gold solution that particle diameter is 15～20nm, the sal tartari (K that is 0.1M by concentration
2cO
3) solution regulates pH value to 8.5～9.5 of colloidal gold solution, must regulate colloidal gold solution B afterwards;
After joining and regulate, bovine serum albumin(BSA) (BSA) antibody-solutions that the mark ratio of 1:1000～1:3000 of take is 1mg/mL by concentration in colloidal gold solution B, under room temperature, reacts 15min; Under 4 ℃ of conditions of temperature, after the centrifugal 20min of speed with 10000rpm, remove unconjugated bovine serum albumin(BSA) (BSA) antibody in supernatant;
The centrifugal precipitation obtaining is scattered in phosphate buffer solution (PBS) again, obtains gold mark amplification of signal probe;
(3) preparation of target detection thing immunological probe gold mark pad and target detection thing amplification of signal gold mark pad
Target detection thing gold-marking immunity probe and gold mark amplification of signal probe are sprayed to respectively on two glass fibre membranes, obtain target detection thing immunological probe gold mark pad and target detection thing amplification of signal gold mark pad;
(4) there is the preparation of the nitrocellulose membrane of detection line and line of reference
The goat anti-rabbit antibody solution that the target detection thing-ovalbumin conjugate solution that is 1mg/mL by concentration on a nitrocellulose membrane and concentration are 1mg/mL is drawn respectively the line of two line parallels, and target detection thing-ovalbumin conjugate is rule as detection line,
Goat anti-rabbit antibody solution is scribed ss line of reference;
(5) preparation of signal enhancement mode immunochromatographigold gold-labeled test strip
Nitrocellulose membrane and five kinds of adhesive matter of water adsorption glass tunica fibrosa of mother glass tunica fibrosa, the golden mark pad of target detection thing immunological probe, target detection thing amplification of signal gold mark being padded, have to detection line and line of reference paste on plastics lining board respectively successively; The length of the lap between adjacent adhesive matter is 2mm, obtains signal enhancement mode super sensitivity detection gold label test strip, and dry encapsulation, preserves under 4 ℃～normal temperature condition of temperature.
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