CN104950108A - Liquid direction cup and detection test paper strip in liquid detection cup - Google Patents
Liquid direction cup and detection test paper strip in liquid detection cup Download PDFInfo
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- CN104950108A CN104950108A CN201510372991.0A CN201510372991A CN104950108A CN 104950108 A CN104950108 A CN 104950108A CN 201510372991 A CN201510372991 A CN 201510372991A CN 104950108 A CN104950108 A CN 104950108A
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The invention relates to a liquid direction cup and a detection test paper strip in the liquid detection cup and belongs to the technical field of bioinstrumentation. The detection test paper strip comprises a bottom plate, wherein a sample cushion, at least two mark cushions, a covering film and water absorption paper are overlapped with one another on the bottom plate; detection lines and quality control lines are arranged on the covering film; the at least two mark cushions are overlapped with one another. According to the liquid direction cup and the detection test paper strip, the at least two mark cushions are arranged and are overlapped with one another, so that the problem that chromatography is blocked as the test paper strip is completely sealed in the prior art can be relatively well solved, and the advantage that the sample feeding success rate is high can be realized.
Description
Technical field
The present invention relates to technical field of biological, particularly relate to a kind of liquid detection cup and test strip wherein.
Background technology
Existing colloidal gold immunochromatographimethod liquid detection cup, can be used for carrying out qualitative to the liquid such as urine, blood or quantitatively detecting, as carried out the qualitative detection of drugs project to urine.This detection cup wall of cup is provided with test strip clamp plate, this test strip clamp plate is provided with the test paper slot placing test strips, the test strips of test is embedded in this test paper slot and uses, this test strips is overlapped in turn to be pasted onto on base plate by sample pad, label pad, nitrocellulose filter, thieving paper and forms, and is conventional test strips.
When needs detect, giving detecting urine cup the patient needing to detect, allowing it fill urine voluntarily, and patient often has little understanding to the structure of test strips, principle, when filling urine, very likely urine can be installed to more than MAX line, cause test strips to lose efficacy, cannot detection be completed.
In order to solve the problem, people are through research and development, improvement has been made to this detection cup, namely faced by test strips, the one side of liquid has set up transparent film layer, this transparent film layer is covered in test strips surface, thus test strips is all covered, or cover major part, only leave bottom part sample application zone and directly contact with liquid.Therefore, when detecting cup built with liquid, test strips top seal is got up by transparent film layer, and only leaving sample application zone directly can contact with liquid, thus ELISA test strip will be made invalid more than MAX line due to liquid, and then guarantees the effective loading of test strips, normal reaction.
When utilizing the detection cup after this improvement to carry out as urine detection, general elder generation loads urine in this detection cup, urine contact is to test strips sample application zone, and in test strips chromatography, detection zone from sample application zone chromatography to label pad, nitrocellulose filter and quality control region, thieving paper, and develop the color on detection zone and quality control region, display result.
But, we find, be pasted together because the various piece in test strips overlaps in turn, usually, a sample application zone part is overlapped in label pad, label pad one of being connected with sample application zone is then overlapped on PVC base plate, and the other end of label pad is overlapped on nitrocellulose filter, thus guarantees that every part can be fixed in PVC board.And chromatography process is: when test strips loading just starts, certain gap is there is between test strips surface and cover layer, in test strips, gas extrudes by environmental liquids, part can be discharged by this gap, but undergoes rapid expansion after test strips water suction, and the test strips of coverlay and imbibition fits tightly, this gap is filled disappearance soon, in test strips, gas cannot be discharged from here again, and so far, test strips is completely sealed.And be occur by capillary action because test strips chromatography reacts, as gaseous tension in test strips is greater than the pulling force of capillary action generation, if namely capillary action pulling force cannot overcome the resistance that in test strips, gas produces, the chromatography effect of test strips will be hindered, and normally cannot carry out chromatography.So cause sample liquid can not continue chromatography occurs under the tractive force effect of thieving paper, and chromatography is subject to hindering and slack-offly even stops, and cause the stopping of reaction, detect unsuccessfully, therefore, use this detection cup to detect, loading success ratio is lower.
Summary of the invention
Based on this, be necessary for the problems referred to above, a kind of liquid detection cup and test strip are wherein provided, adopt this test strips and detect cup, chromatography process is not interrupted, thus both ELISA test strip will be made invalid more than MAX line due to testing liquid, the effective loading of test strips can be guaranteed again, normal reaction.
A kind of test strip, comprise base plate, described base plate is overlapped with sample pad, label pad, coated film and thieving paper successively mutually, described coated film is provided with detection line and nature controlling line, and described label pad is at least two pieces, and overlap use mutually.
Inventor finds under study for action, there is test strips and sealed in conventional detection cup, the main cause that chromatography is obstructed is, because the label pad in test strips has certain thickness, front and back are overlapping with sample pad and coated film again, therefore, overlapping portion is higher relative to other parts of test strips, and steep bad test strips for avoiding testing liquid to rush sample, the degree of depth that test paper slot is arranged has a definite limitation, and also post transparent mulch film above slot, therefore, when sample liquid is filled into after in urine cup, because label pad part is natively higher, little with coverlay gap, testing liquid loading chromatography is through label pad, label pad segment thickness is high, inhale sample also many, label pad undergoes rapid expansion also very fast and coverlay is fitted, seal test strips, in test strips, original gas can not effectively be discharged outside the confined space that test strips and coverlay formed, internal pressure is large and cause chromatography to be obstructed.
That is, test strips is sealed the main cause causing chromatography to be obstructed and is: during loading chromatography, test strips seals by label pad part, therefore, solves above-mentioned chromatography and to be obstructed the key of problem, be just that how avoiding label pad to expand causes sealing test strips.
From above-mentioned result of study, the present inventor is drawn after being groped by a large amount of experiments, label pad is set at least two pieces, and overlap use mutually, the problem of label pad Expansion sealing test strips can be solved preferably, this is because: label pad is at least two pieces by a complete block comminute, and overlap joint uses, so relatively large gap will be produced in the part of overlap joint, thus for label pad expection expansion headspace, even if make label pad expand, also can not fit completely with coverlay, test strips is sealed completely.
Wherein in an embodiment, described label pad is two pieces, overlaps successively along test strips chromatography direction.Adopt two pieces of label pad, necessary reserved expansion space can be provided for label pad, label pad can be avoided again to be separated into too much part, affect the effect that testing liquid chromatography in test strips climbs, and label pad is overlapped along test strips chromatography direction, is beneficial to carrying out smoothly of chromatography process.
Wherein in an embodiment, the length of the two pieces of label pad overlapped successively is than being 1:0.8-1.2.The length of two pieces of label pad is set to basically identical, the position namely making two pieces of label pad mutually overlap is positioned at the centre position of conventional non-dividing mark pad, namely most probable fit with coverlay owing to expanding, and seals the position of test strips, thus can solve chromatography preferably and to be obstructed problem.
Wherein in an embodiment, the thickness of described label pad is 0.2-1.3mm, and mutually the lap of overlap joint is the 1/5-4/5 of label pad length, preferred 1/4-3/4, more preferably 1/3-3/5, most preferably 1/2.The present inventor finds in practice, as lap is longer, is equivalent to the thickness adding label pad, overlap as the reserved insufficient space of the expansion of label pad expection, and due to the increase of thickness, degrees of expansion also can increase greatly, still not can solve chromatography and to be obstructed problem.Further, the above-mentioned lap of splice also affects the steadiness of test strips, and the length as mutually overlapped is longer, then the length being correspondingly pasted onto the label pad part on base plate declines, fixing unstable, and loading chromatography success ratio also can be caused to decline.But the thickness of label pad is different, the best lap of splice with matching is also not identical, and the thickness of label pad and the lap of splice is arranged according to above-mentioned parameter, has best chromatographic effect.
Wherein in an embodiment, the thickness of described sample pad is 0.2-1.3mm, and the lap that described label pad and sample pad overlap mutually is the 1/4-5/6 of label pad length, preferred 1/2-5/6.The present inventor also finds in practice, and the overlap joint between sample pad with label pad coordinates and also can impact chromatography process, according to above-mentioned setting, can ensure chromatographic effect further.
Wherein in an embodiment, this test strips also comprises fixing film, and described fixing film is covered in the mutual lap position place of sample pad, label pad and coated film.This fixing film is except better can fixing the sample pad, label pad and the coated film that mutually overlap, can also play and slow down, control the Tomography Velocity of testing liquid in test strips, prevent the too fast test strips that causes of testing liquid Tomography Velocity from rushing sample, the situation of reacting failed occurs.
Wherein in an embodiment, described coated film is nitrocellulose filter.
The invention also discloses a kind of liquid detection cup, comprise cup, test strip clamp plate, coverlay and above-mentioned test strips; Described test strip clamp plate is placed in cup, which is provided with test paper slot, and described test strips is placed in described test paper slot, and described coverlay is covered in described test strips surface by test paper slot opening surface.
Aforesaid liquid detects cup, owing to have employed the test strip of above-mentioned improvement, when testing liquid being detected with this liquid detection cup, liquid is filled in cup, testing liquid touches test strips sample pad, start chromatography in test strips, from sample pad chromatography to label pad, along with the carrying out of chromatography, test strips imbibition, label pad also expands gradually, but because the label pad of overlap joint creates relatively large gap, thus provide space for the expansion of label pad, can avoid test strips being sealed the chromatography caused to be obstructed problem because label pad expanding, again because coverlay is protected test strips, reach and both ELISA test strip will be made invalid more than MAX line due to testing liquid, the effective loading of test strips can be guaranteed again, the object of normal reaction.
Wherein in an embodiment, the length of described coverlay bottom edge distance test strips bottom margin is the 1/6-5/6 of sample pad length.The bottom edge reference position of coverlay is very crucial to the quality of chromatographic effect, coverlay is allowed to cover a part of sample pad, instead of test strips is covered or only covering by label pad completely, object makes testing liquid have a buffer action when test strips loading, testing liquid can get over labeling pad by chromatography effect, then in viewing area display result.As test strips covered completely, be then difficult to the loading that assures success; As coverlay only overlay marks pad district and with upper part, then the sample pad of steeping in liquid is oversize, and liquid directly may break through label pad, rushes sample phenomenon, and causing chromatography to react can not normally carry out.
Wherein in an embodiment, described coverlay is hyaline membrane.Be convenient to observe detection procedure and result.
Compared with prior art, the present invention has following beneficial effect:
A kind of test strip of the present invention, label pad is set at least two pieces, and overlap use mutually, so relatively large gap will be produced in the part of overlap joint, thus for label pad expection expansion headspace, even if make label pad expand, also can not fit completely with coverlay, test strips is sealed completely.Thus solve in routine techniques and to be obstructed problem because test strips is completely sealed the chromatography caused, there is the advantage that loading success ratio is high.
Further, the present invention is designed by repetition test and verifies, finally determines best label pad overlap joint fit system, and the parameter such as the overlap joint thickness of the best and length, can obtain best test result with the chromatography process of optimum.
A kind of liquid detection cup of the present invention; have employed the test strip of above-mentioned improvement, and the coverlay of protection can be provided test strips, both ELISA test strip will be made invalid more than MAX line due to testing liquid; the effective loading of test strips can be guaranteed again, there is good chromatographic effect.
Accompanying drawing explanation
Fig. 1 is the structural representation of test strips in embodiment 1;
Fig. 2 is that in embodiment 1, test strips and fixing film coordinate rear structural representation;
Fig. 3 is part A partial enlarged drawing in Fig. 2;
Fig. 4 is the schematic perspective view of Fig. 2;
Fig. 5 is part B partial enlarged drawing in Fig. 4;
Fig. 6 is the front Each part schematic diagram of test strips assembling in embodiment 1;
Fig. 7 is liquid detection cup structural representation in embodiment 2;
Fig. 8 is that in embodiment 2, test strip clamp plate, test strips and coverlay coordinate schematic diagram.
Wherein: 100. test strips; 110. base plate; 120. sample pad; 131. first label pad; 132. second label pad; 140. coated film; 150. thieving paper; 160. fixing films; 200. coverlay; 300. test strip clamp plate; 310. test paper slots; 400. cup.
Embodiment
Below in conjunction with specific embodiments and the drawings, the present invention will be further explained illustrates, but do not cause any restriction to the present invention.
Embodiment 1
A kind of test strip, as shown in Figure 1, comprise base plate 110, described base plate 110 is overlapped with sample pad 120 successively mutually, label pad (131, 132), coated film 140 and thieving paper 150, described coated film 140 is provided with detection line and nature controlling line, in the present embodiment, described coated film is nitrocellulose filter, described label pad is two pieces (being respectively the first label pad 131 and the second label pad 132), overlap along test strips chromatography direction use successively, and, these two pieces of label pad to be blocked from centre by the label pad of routine to obtain, its length is 4mm, as shown in Figure 6.
In the present embodiment, the thickness of described label pad is 0.3mm, and length is respectively 4mm, and the length of overlapped part of overlap joint is 2mm mutually, is 1/2 of label pad length.The thickness of described sample pad is 0.3-1mm, and the length of overlapped part that described sample pad and label pad overlap mutually is 2mm.Understandable, also can adjust overlap length according to different label pad and sample pad thickness, but, arrange according to above-mentioned parameter, there is best chromatographic effect.
In the present embodiment, this test strips also comprises fixing film 160, and described fixing film 160 is covered in sample pad 120, label pad (131,132) and coated film 140 mutually lap position place, as shown in Figure 2, this fixing film 160 is transparent adhesive film, has the advantage be conveniently easy to get.
In the present embodiment, it is that length is respectively two pieces of 4mm that label pad is grown a complete block comminute by 8mm, and overlap joint uses, so relatively large gap will be produced in the part of overlap joint, as in Figure 3-5, thus for label pad expection expansion headspace, even if make label pad expand, also can not fit completely with coverlay, test strips is sealed completely.
Embodiment 2
A kind of liquid detection cup, as Figure 7-8, comprises the test strips 100 in cup 400, test strip clamp plate 300, transparent mulch film 200 and embodiment 1; Described test strip clamp plate 300 is placed in cup 400, which is provided with test paper slot 310, and described test strips 100 is placed in described test paper slot 310, and described coverlay 200 is covered in described test strips 100 surface by test paper slot opening surface.
In the present embodiment, the length of described coverlay bottom edge distance test strips bottom margin is 2/3 of sample pad length.
Embodiment 3
A kind of liquid detection cup, substantially identical with the liquid detection cup of embodiment 2, the key distinction is:
The length of described label pad is respectively 5mm, and the length of overlapped part of overlap joint is 2.5mm mutually.The length of overlapped part that described sample pad and label pad overlap mutually is 3mm, is 3/5 of label pad length.
Embodiment 4
A kind of liquid detection cup, substantially identical with the liquid detection cup of embodiment 2, the key distinction is:
The length of overlapped part that described label pad overlaps mutually is 3mm, is 3/4 of label pad length.
Embodiment 5
A kind of liquid detection cup, substantially identical with the liquid detection cup of embodiment 2, the key distinction is:
The length of overlapped part that described label pad overlaps mutually is 1mm, is 1/4 of label pad length.
Embodiment 6
A kind of liquid detection cup, substantially identical with the liquid detection cup of embodiment 2, the key distinction is:
Described label pad is three pieces, and overlap along test strips chromatography direction use successively, and these three pieces of label pad to be blocked from centre by the label pad of routine to obtain, and its length is 3.33mm, and the length of overlapped part of overlap joint is 1.5mm mutually.
Experimental example
The detection cup being directly provided with coverlay in the liquid detection cup of embodiment 2-6 and routine techniques on test strips surface is adopted to carry out chromatography test.
This experiment is by the 1000 increments bases to collection, and employing specification, the test strips that test item type is identical are tested, and investigate its effect, experimental result is as shown in table 1 below.
Table 1. chromatography test result
Can be found out by the above results, use the detection cup in routine techniques, because test strips internal pressure is excessive, the chromatography effect of part test strips is caused to be hindered, even cannot successful loading, namely tracer liquid can not climb precalculated position, and control zone cannot be made to produce respective change, loading success ratio is lower, is only about 80%.
And the detection cup of embodiment of the present invention 2-6, owing to have employed the label pad of mutually overlap joint, space has been reserved in the expansion for label pad, solves chromatography to be obstructed problem, and its loading success ratio is all high than the detection cup success ratio in routine techniques.
And owing to overlapping the difference of the optimum configurations such as overlapping length, the steadiness of test strips is also different.As the detection cup in embodiment 4, although higher compared to the loading success ratio of conventional sense cup, the length mutually overlapped due to label pad is longer, be pasted onto the corresponding reduction of length of the label pad part on base plate, fixing unstable, its structure is loose compared with embodiment 2, and steadiness is not as embodiment 2.For another example the detection cup in embodiment 5, although higher compared to the loading success ratio of conventional sense cup, but the length mutually overlapped due to label pad is shorter, the space overlapped as the expansion of label pad expection is reserved is less, deficiency is thought that the suction sample of label pad expands and is reserved enough spaces, therefore, the success ratio of loading and chromatography reaction is relatively low.
And the detection cup of embodiment 2, selected best label pad overlap joint fit system and the parameter such as the overlap joint thickness of the best and length, the structural soundness of test strips is good, has best chromatographic effect simultaneously, and loading success ratio reaches 100%.
For the detection cup in embodiment 6, due to label pad is cut into three pieces, thus label pad has overlapped 2 times mutually, and original label pad length is constant, label pad total length after overlap joint shortens, and is only 5mm, and every block label pad to overlap the length be fixed on base plate comparatively limited, steadiness is poor, also affects chromatography process.
Each technical characteristic of the above embodiment can combine arbitrarily, for making description succinct, the all possible combination of each technical characteristic in above-described embodiment is not all described, but, as long as the combination of these technical characteristics does not exist contradiction, be all considered to be the scope that this instructions is recorded.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be construed as limiting the scope of the patent.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. a test strip, is characterized in that, comprises base plate, described base plate is overlapped with sample pad, label pad, coated film and thieving paper successively mutually, described coated film is provided with detection line and nature controlling line, and described label pad is at least two pieces, and overlap use mutually.
2. test strip according to claim 1, is characterized in that, described label pad is two pieces, overlaps successively along test strips chromatography direction.
3. according to test strip according to claim 2, it is characterized in that, the length of the two pieces of label pad overlapped successively is than being 1:0.8-1.2.
4. the test strip according to any one of claim 1-3, is characterized in that, the thickness of described label pad is 0.2-1.3mm, and the lap of overlap joint is the 1/5-4/5 of label pad length mutually.
5. test strip according to claim 4, is characterized in that, the thickness of described sample pad is 0.2-1.3mm, and the lap that described label pad and sample pad overlap mutually is the 1/4-5/6 of label pad length.
6. the test strip according to any one of claim 1-3, is characterized in that, also comprises fixing film, and described fixing film is covered in the mutual lap position place of sample pad, label pad and coated film.
7. test strip according to claim 1, is characterized in that, described coated film is nitrocellulose filter.
8. a liquid detection cup, is characterized in that, comprises cup, test strip clamp plate, coverlay and the test strips described in any one of claim 1-7; Described test strip clamp plate is placed in cup, which is provided with test paper slot, and described test strips is placed in described test paper slot, and described coverlay is covered in described test strips surface by test paper slot opening surface.
9. liquid detection cup according to claim 8, is characterized in that, the length of described coverlay bottom edge distance test strips bottom margin is the 1/6-5/6 of sample pad length.
10. liquid detection cup according to claim 8, is characterized in that, described coverlay is hyaline membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510372991.0A CN104950108A (en) | 2015-06-26 | 2015-06-26 | Liquid direction cup and detection test paper strip in liquid detection cup |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510372991.0A CN104950108A (en) | 2015-06-26 | 2015-06-26 | Liquid direction cup and detection test paper strip in liquid detection cup |
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| CN107966561A (en) * | 2017-11-24 | 2018-04-27 | 德诺杰亿（北京）生物科技有限公司 | A kind of test strips and kit for detecting HIV antibody |
| CN109100346A (en) * | 2017-06-21 | 2018-12-28 | 上海溯源生物技术有限公司 | Test paper bar assembly |
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Application publication date: 20150930 |
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