CN106771180A - A kind of test strips for detecting Goose Parvovirus antibody - Google Patents
A kind of test strips for detecting Goose Parvovirus antibody Download PDFInfo
- Publication number
- CN106771180A CN106771180A CN201611037160.9A CN201611037160A CN106771180A CN 106771180 A CN106771180 A CN 106771180A CN 201611037160 A CN201611037160 A CN 201611037160A CN 106771180 A CN106771180 A CN 106771180A
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- Prior art keywords
- test strips
- support
- gold standard
- absorbent filter
- layer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of test strips for detecting Goose Parvovirus antibody, including test strips body, the test strips body includes epiphragma, sample pad, gold standard pad layer, absorbent filter, Handheld Division, detection zone and the fixed viscous lining of support, epiphragma is provided with the upper surface of test strips body, the bottom of test strips body is set to the fixed viscous lining of support, sample pad is provided with the fixed viscous lining of support, gold standard pad layer, absorbent filter and detection zone, sample pad and absorbent filter are separately positioned on the two ends at the top of the fixed viscous lining of support, the support of sample pad side is fixed and gold standard pad layer is provided with viscous lining, detection zone is provided between gold standard pad layer and absorbent filter, it is provided with test strips body on one end of absorbent filter and is provided with Handheld Division.The present invention accelerates detection speed by the heat conducting film and heating film that set, expands the use scope of test strips, and "T"-shaped detection line and " C " form quality inspection line coordinate, be easy to intuitively understand testing result.
Description
Technical field
The present invention relates to Lateral Flow Strip field, specially a kind of test strips for detecting Goose Parvovirus antibody.
Background technology
Goose Parvovirus, also known as goose parvovirus, can cause a kind of acute or subacute septicemia gosling plague of young goose, with
The exudative enteritis of generation is main pathological change.This disease mainly encroaches on 4-20 ages in days young bird goose, infects fast, case fatality rate high.This disease is most
Early to be found in China Operation in Yangzhou Area in 1956 by Chinese scholar Fang Dingyi, most domestic supports goose provinces and regions generation, provisions
Goose industry causes Important Economic to lose.Quick detection Goose Parvovirus antibody, can not only evaluate immune effect of vaccine, and can be with
Adjuvant clinical is diagnosed, and is the premise of effectively control Goose Parvovirus infection.
Existing test strips have detection specificity and sensitiveness is poor；When detection environment temperature is relatively low, extension detection
Time and influence testing result precision, the use scope of test strips is smaller；Antigen-antibody reaction is entered in liquid phase environment
Row detection, causes the detection speed of test strips slower, complex structure, understanding of the inconvenience to result；When being loaded in sample pad,
The excessive pollution caused to other parts, so that the problems such as influenceing the accuracy of testing result.
The content of the invention
It is an object of the invention to provide a kind of test strips for detecting Goose Parvovirus antibody, the detection speed for possessing is fast,
Accuracy advantage high, solve the detection speed that existing test strip caused due to the influence of the factors such as temperature it is slow,
The relatively low problem of accuracy.
To achieve the above object, the present invention provides following technical scheme：A kind of test strips for detecting Goose Parvovirus antibody,
Including test strips body, the test strips body includes epiphragma, sample pad, gold standard pad layer, absorbent filter, Handheld Division, detection zone
Viscous lining fixed with support, is provided with epiphragma on the upper surface of test strips body, the bottom of test strips body is set to support admittedly
Surely lining is glued, sample pad, gold standard pad layer, absorbent filter and detection zone, sample pad and water suction is provided with the fixed viscous lining of support
Filter paper is separately positioned on the two ends at the top of the fixed viscous lining of support, and the support of sample pad side is fixed on viscous lining and is provided with golden mark
Bed course, detection zone is provided between gold standard pad layer and absorbent filter, is provided with test strips body on one end of absorbent filter and set
It is equipped with Handheld Division.
Preferably, the fixed viscous lining of the support includes heat conducting film, heating film and PVC backer boards, and PVC backer boards are arranged on
The bottom of the fixed viscous lining of support, heating film is set between PVC backer boards and heat conducting film.
Preferably, the gold standard pad layer includes polyester fiber film and gold labeling antibody composite layer, and polyester fiber film is arranged on
The upside of gold labeling antibody composite layer.
Preferably, the sample pad is arranged in the groove on the fixed viscous lining of support, and the both sides of sample pad are provided with leads
Chute.
Preferably, "T"-shaped detection line is provided with the detection zone and " C " form quality examines line, "T"-shaped detection line is arranged on to be leaned on
The side of nearly gold standard pad layer, " C " form quality inspection line is positioned close to the side of absorbent filter, detection zone and the fixed viscous lining of support it
Between be provided with capillary chromatography reaction solid-phase layer.
Preferably, the gold that is internally provided with of the capillary chromatography reaction solid-phase layer marks protein solution coating, gold mark albumen
Solution coatings are positioned close on the side of gold standard pad layer.
Compared with prior art, beneficial effects of the present invention are as follows：
1st, the gold standard pad layer and gold mark protein solution coating, polyester fiber film and gold labeling antibody composite layer that the present invention passes through setting
Coordinate, effectively increase the detection specificity and sensitiveness of test strips, simple structure is easy to use.
2nd, the present invention, can be to test strips in detection by being arranged on the fixed heat conducting film and heating film glued in lining of support
When carry out appropriate heating, it is to avoid detection environment temperature it is relatively low extension detection time and influence testing result precision, expand
The use scope of big test strips, is easy to whenever and wherever possible carry out Site Detection.
3rd, the present invention reacts solid-phase layer and detection zone, capillary chromatography reaction solid-phase layer profit by the capillary chromatography for setting
With the effect of capillary, antigen-antibody reaction is gone to and quickly carried out on solid phase filter membrane, effectively accelerate the detection speed of test strips
Degree, "T"-shaped detection line and " C " form quality inspection line coordinate, and are easy to intuitively understand testing result, time-consuming, simple structure,
It is easy to use.
4th, the present invention is by the groove and guiding gutter that set, it is to avoid when being loaded in sample pad, can excessively cause to it
The pollution of his part, so as to influence the accuracy of testing result.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention；
Fig. 2 is front view of the present invention；
Fig. 3 is structure sample pad schematic diagram of the present invention.
In figure：1- test strips bodies, 2- epiphragmas, 3- sample pads, 4- gold standard pads layer, 41- polyester fiber films, 42- gold marks are anti-
Bluk recombination nitride layer, the "T"-shaped detection lines of 5-, 6- " C " form quality inspection line, 7- absorbent filters, 8- Handheld Divisions, 9- detection zones, 10- gold mark eggs
White solution coatings, the fixed viscous lining of 11- supports, 111- heat conducting films, 112- heating films, 113-PVC backer boards, 12- capillary fringes
Analysis reaction solid-phase layer, 13- grooves, 14- guiding gutters.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Fig. 1-3 are referred to, the present invention provides a kind of technical scheme：A kind of test strips for detecting Goose Parvovirus antibody, bag
Test strips body 1 is included, the test strips body 1 includes epiphragma 2, sample pad 3, gold standard pad layer 4, absorbent filter 7, Handheld Division 8, inspection
Area 9 and the fixed viscous lining 11 of support are surveyed, epiphragma 2 is provided with the upper surface of test strips body 1, the bottom of test strips body 1 sets
The fixed viscous lining 11 of support is set to, sample pad 3, gold standard pad layer 4, absorbent filter 7 and inspection are provided with the fixed viscous lining 11 of support
Area 9 is surveyed, sample pad 3 and absorbent filter 7 are separately positioned on the two ends at the fixed viscous top of lining 11 of support, the branch of the side of sample pad 3
Gold standard pad layer 4 is provided with the fixed viscous lining 11 of support, detection zone 9, test strips are provided between gold standard pad layer 4 and absorbent filter 7
It is provided with body 1 and Handheld Division 8 is provided with one end of absorbent filter 7.
The fixed viscous lining 11 of support includes heat conducting film 111, heating film 112 and PVC backer boards 113, PVC backer boards
113 bottoms for being arranged on the fixed viscous lining 11 of support, are set to heating film 112 between PVC backer boards 113 and heat conducting film 111, can
Appropriate heating is carried out when detection to test strips, it is to avoid the time of the relatively low extension detection of detection environment temperature and influence are examined
The precision of result is surveyed, expands the use scope of test strips, the gold standard pad layer 4 includes that polyester fiber film 41 and gold labeling antibody are compound
Nitride layer 42, polyester fiber film 41 is arranged on the upside of gold labeling antibody composite layer 42, and it is fixed viscous that the sample pad 3 is arranged on support
In groove 13 on lining 11, the both sides of sample pad 3 are provided with guiding gutter 14, it is to avoid excessive when toward sample-adding in sample pad, make
The pollution of paired other parts, so as to influence the accuracy of testing result, is provided with the He of "T"-shaped detection line 5 on the detection zone 9
" C " form quality examines line 6, and "T"-shaped detection line 5 is positioned close to the side of gold standard pad layer 4, and " C " form quality inspection line 6 is positioned close to water suction
The side of filter paper 7, capillary chromatography reaction solid-phase layer 12, capillary are provided between detection zone 9 and the fixed viscous lining 11 of support
Antigen-antibody reaction is gone to and quickly carried out on solid phase filter membrane by chromatography reaction solid-phase layer 12 using the effect of capillary, is effectively added
The detection speed of fast test strips, "T"-shaped detection line 5 and " C " form quality inspection line 6 coordinate, and are easy to intuitively understand testing result,
Time-consuming, the gold that is internally provided with of the capillary chromatography reaction solid-phase layer 12 marks protein solution coating 10, and gold mark albumen is molten
Liquid coating 10 is positioned close on the side of gold standard pad layer 4, effectively increases the detection specificity and sensitiveness of test strips, structure
Simply, it is easy to use.
Operation principle：This kind detects the test strips of Goose Parvovirus antibody, during detection, is dripped in the sample pad 3 of test strips
Plus 2~3 drop sample liquids, observation result in 2~5min, "T"-shaped detection line 5 and " C " form quality inspection line 6 occur red being the positive,
Represent there is detection antiviral antibody in sample is detected, it is feminine gender that only " C " form quality inspection line 6 occurs red, is represented in tested test sample
Antiviral antibody is not detected in product liquid, it is invalid that "T"-shaped detection line 5 and " C " form quality inspection line 6 do not occur red then test paper.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding can carry out various changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (6)
1. a kind of test strips for detecting Goose Parvovirus antibody, including test strips body（1）, it is characterised in that：The test strips
Body（1）Including epiphragma（2）, sample pad（3）, gold standard pad layer（4）, absorbent filter（7）, Handheld Division（8）, detection zone（9）And branch
The fixed viscous lining of support（11）, test strips body（1）Upper surface on be provided with epiphragma（2）, test strips body（1）Bottom set
It is the fixed viscous lining of support（11）, the fixed viscous lining of support（11）On be provided with sample pad（3）, gold standard pad layer（4）, absorbent filter
（7）And detection zone（9）, sample pad（3）And absorbent filter（7）It is separately positioned on the fixed viscous lining of support（11）The two ends at top,
Sample pad（3）Viscous lining is fixed in the support of side（11）On be provided with gold standard pad layer（4）, gold standard pad layer（4）And absorbent filter（7）
Between be provided with detection zone（9）, test strips body（1）On be provided with absorbent filter（7）One end on be provided with Handheld Division（8）.
2. it is according to claim 1 it is a kind of detect Goose Parvovirus antibody test strips, it is characterised in that：The support is solid
Surely lining is glued（11）Including heat conducting film（111）, heating film（112）With PVC backer boards（113）, PVC backer boards（113）It is arranged on
The fixed viscous lining of support（11）Bottom, PVC backer boards（113）And heat conducting film（111）Between be set to heating film（112）.
3. it is according to claim 1 it is a kind of detect Goose Parvovirus antibody test strips, it is characterised in that：The gold standard pad
Layer（4）Including polyester fiber film（41）With gold labeling antibody composite layer（42）, polyester fiber film（41）Gold labeling antibody is arranged on to answer
Compound layer（42）Upside.
4. it is according to claim 1 it is a kind of detect Goose Parvovirus antibody test strips, it is characterised in that：The sample pad
（3）It is arranged on the fixed viscous lining of support（11）On groove（13）It is interior, sample pad（3）Both sides be provided with guiding gutter（14）.
5. it is according to claim 1 it is a kind of detect Goose Parvovirus antibody test strips, it is characterised in that：The detection zone
（9）On be provided with "T"-shaped detection line（5）" C " form quality examines line（6）, "T"-shaped detection line（5）It is positioned close to gold standard pad layer（4）
Side, " C " form quality inspection line（6）It is positioned close to absorbent filter（7）Side, detection zone（9）Viscous lining fixed with support
（11）Between be provided with capillary chromatography reaction solid-phase layer（12）.
6. it is according to claim 5 it is a kind of detect Goose Parvovirus antibody test strips, it is characterised in that：The capillary
Chromatography reaction solid-phase layer（12）Be internally provided with gold mark protein solution coating（10）, gold mark protein solution coating（10）It is arranged on
Near gold standard pad layer（4）Side on.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611037160.9A CN106771180A (en) | 2016-11-23 | 2016-11-23 | A kind of test strips for detecting Goose Parvovirus antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611037160.9A CN106771180A (en) | 2016-11-23 | 2016-11-23 | A kind of test strips for detecting Goose Parvovirus antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106771180A true CN106771180A (en) | 2017-05-31 |
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ID=58973708
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611037160.9A Pending CN106771180A (en) | 2016-11-23 | 2016-11-23 | A kind of test strips for detecting Goose Parvovirus antibody |
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| CN (1) | CN106771180A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108872580A (en) * | 2018-06-19 | 2018-11-23 | 山东农业大学 | A kind of colloidal gold strip and preparation method thereof detecting novel goose parvovirus |
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| CN205539002U (en) * | 2016-03-02 | 2016-08-31 | 河南科技学院 | Detect test paper strip of gosling pestivirus antibody |
| CN205594013U (en) * | 2016-03-18 | 2016-09-21 | 成都农业科技职业学院 | Detection test paper |
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2016
- 2016-11-23 CN CN201611037160.9A patent/CN106771180A/en active Pending
Patent Citations (8)
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|---|---|---|---|---|
| US6338969B1 (en) * | 1999-11-15 | 2002-01-15 | Bayer Corporation | Assay test system for regulating temperature |
| CN102353792A (en) * | 2011-07-07 | 2012-02-15 | 贵州大学 | Indirect ELISA (enzyme linked immunosorbent assay) kit based on GPV (goat poxvirus) P32 protein and preparation method |
| CN102507929A (en) * | 2011-10-28 | 2012-06-20 | 合肥工业大学 | Signal-enhancement type immunochromatographic gold-labeled test strip and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108872580A (en) * | 2018-06-19 | 2018-11-23 | 山东农业大学 | A kind of colloidal gold strip and preparation method thereof detecting novel goose parvovirus |
| CN108872580B (en) * | 2018-06-19 | 2021-07-02 | 山东农业大学 | Colloidal gold test strip for detecting novel goose parvovirus and preparation method thereof |
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Application publication date: 20170531 |