CN205594013U - Detection test paper - Google Patents
Detection test paper Download PDFInfo
- Publication number
- CN205594013U CN205594013U CN201620218241.8U CN201620218241U CN205594013U CN 205594013 U CN205594013 U CN 205594013U CN 201620218241 U CN201620218241 U CN 201620218241U CN 205594013 U CN205594013 U CN 205594013U
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- China
- Prior art keywords
- piglet
- pad
- line
- test paper
- isospora
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Abstract
The utility model discloses a detection test paper, including PVC backing plate to and the linking sets up sample pad, gold mark pad, NC membrane and the filter paper that absorbs water on PVC backing plate in proper order, and the epimembranal surface of NC is provided with detection line and quality testing line, and is gapped between detection line and the quality testing line, and the quality testing line is kept away from golden mark and is filled up. The utility model discloses simple structure, convenient to use detects fast, and the diagnosis is accurate.
Description
Technical field
This utility model relates to immune antibody detection technique field, particularly relates to a kind of Test paper.
Background technology
Pig coccidiosis is by Isospora suis (isospara suis) and some Eimeria (Eimeria)
Coccidiosis parasitize caused by the Small Intestine of Piglets epithelial cell of age of sucking and wean based on diarrhoea
Wanting the protozoacide of clinical symptoms, wherein piglet isospora (isospara suis) is to cause pig ball
The main pathogen of parasitosis, and be often constitutional, betides the age of sucking in delivery room or new wean
Piglet, 7 14 age in days piglets are the most susceptible.Isospora often parasitizes age of sucking and the son that just weaned
On the intestinal epithelial cell of pig, piglet can be made to suffer from diarrhoea, become thin, be dehydrated and dead, be mesh
The formidable enemy of front large scale of pig farm, popular relatively wide, harm is big.General piglet occurs diarrhoea all to be neglected
Once occur that diarrhoea is taken as antibacterial or virus infects depending on, piglet, as yellow scour of piglet, Hakuri,
Transmissible gastroenteritis of swine, piglet epidemic diarrhea etc., with antibiotic or antiviral treatment without
Effect, makes the course of disease extend or dead, causes serious economic loss.About the epidemiology of primary disease,
Having many research reports in recent years, the epidemiological study of national multiple provinces and cities is shown by Chinese scholar,
The infection rate of China's piglet Isospora coccidiosis 7.02%～45%, and this disease Europe, the United States, Australia,
Southeast Asian countries and regions are popular the most extremely serious, and the abundant of person is paid close attention to cause veterinary parasitology,
Many progress are achieved at aspects such as nosetiology, epidemiology, immunologys.In countries in the world,
Its incidence rate accounts in piglet diarrhea disease 15% 20%.Epidemic diseases according to European countries
Learning investigation has the pig farm of 83%～100% to have the infection of piglet isospora, leads every year in the U.S.
The economic loss caused is up to more than 100,000,000 dollars.
Before and after piglet Isospora coccidiosis betides age of sucking and wean, the height of piglet diarrhoea just
In the stage of sending out, there are multiple pathology or physiologic factor can cause piglet diarrhea.Therefore to coccidiasis in piglets on some farms
Diagnosis relatively difficult, from excrement examined, only discover and seize egg capsule or to carry out gram feces egg capsule counting be inadequate
, it is necessary to it is aided with and cuts open inspection, look in intestinal epithelial cell and see Eimeria or isospora
Endogenous stage polypide and corresponding pathological change just can be made a definite diagnosis.But this diagnostic method needs relatively
High clinical technology level, the veterinary arts level of relative of current China is relatively low, it is therefore desirable to seek
Ask method the simplest, accurate, easy-operating to diagnose this class disease, but up to the present,
Domestic research report is adjusted primarily with regard to biological characteristics, the epidemiology of piglet isospora
Looking into and the report of the aspect such as Drug therapy, the research about diagnosis reagent paper is less.
Utility model content
This utility model aims to provide a kind of Test paper, and simple in construction is easy to use, diagnosis
Accurately.
For reaching above-mentioned purpose, the technical solution adopted in the utility model is as follows:
Disclosed in this utility model, a kind of Test paper, including PVC backer board, and holds in the mouth successively
Meet and be arranged on the sample pad on PVC backer board, gold mark pad, NC film and absorbent filter, institute
State NC film upper surface and be provided with detection line and quality inspection line, between detection line and quality inspection line, have gap,
Described quality inspection line is away from gold mark pad.
Preferably, described detection line includes anti-piglet isospora antigen layer, described anti-piglet etc.
Sorosphere worm antigen layer is attached on NC film；Described quality inspection line includes anti-piglet isospora antibody
Layer, described anti-piglet isospora antibody layer is attached on NC film.
Preferably, described gold mark pad includes gold labeling antibody composite layer and polyester fiber film, described
Polyester fiber film is attached on PVC backer board, and described gold labeling antibody composite layer is attached to polyester
On fibrous membrane.
Further, described sample pad, gold mark pad, NC film and absorbent filter and PVC backing
Plate is bonding.
Further, described sample pad, gold mark pad, detection line, quality inspection line and absorbent filter row
Row are point-blank.
Further, the size of described PVC backer board is 0.4cm × 8.0cm.
This utility model has the advantages that simple in construction, easy to operate, with low cost,
Quickly, diagnosis is accurately in detection.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the top view of Fig. 1；
In figure: 1-PVC backer board, 2-NC film, 3-sample pad, 4-gold mark pad, 5-detection line,
6-quality inspection line, 7-absorbent filter, 41-polyester fiber film, 42-gold labeling antibody composite layer.
Detailed description of the invention
In order to make the purpose of this utility model, technical scheme and advantage clearer, below
In conjunction with accompanying drawing, this utility model is further elaborated.
As shown in Figure 1 and Figure 2, a kind of Test paper disclosed in this utility model, including PVC
Backer board 1, and be connected successively and be arranged on PVC backer board 1 sample pad 3, gold mark pad
4, NC film 2 and absorbent filter 7, NC film 2 upper surface is provided with detection line 5 and quality inspection line 6,
Having gap between detection line 5 and quality inspection line 6, quality inspection line 6 is away from gold mark pad 4.Further,
Sample pad 3, gold mark pad 4, NC film 2 and absorbent filter 7 are bonding with PVC backer board 1；Sample
Product pad 3, gold mark pad 4, detection line 5, quality inspection line 6 and absorbent filter 7 are arranged in one directly
On line, the outer end of sample pad 3 flushes with one end of PVC backer board 1, outside absorbent filter 7
End flushes with the other end of PVC backer board 1, and the preferred 0.4cm of size of PVC backer board 1 ×
8.0cm。
Detection line 5 includes anti-piglet isospora antigen layer, and anti-piglet isospora antigen layer is attached
On NC film 2, the spores such as quality inspection line 6 includes anti-piglet isospora antibody layer, anti-piglet
Coccidiosis antibody layer is attached on NC film 2.Gold mark pad 4 includes gold labeling antibody composite layer 42 He
Polyester fiber film 41, polyester fiber film 41 is attached on PVC backer board 1, and gold labeling antibody is multiple
Compound layer 42 is attached on polyester fiber film on 41.
Preparation method of the present utility model is as follows:
1. prepared by piglet isospora antigen
Piglet isospora egg capsule phosphate buffer (PBS) of purification is cleaned 3 times by 1.1；
1.2 coccidian oocysts cleaned add phosphate buffer (PBS), ultrasonic degradation 30min, shows
Check under micro mirror that egg capsule crushes situation, after cracking completely, lysate adds reagent
TritonX-100 (final concentration of 0.5%), at 4 DEG C, 12000r/min is centrifuged 30min,
Take supernatant；1.3 using after supernatant aseptic filtration (0.22 μm) as soluble antigen, and
Measure the qualified rear 0.01mol/L PBS liquid of protein content with Coomassie Brilliant Blue to dilute also
It is sub-packed in bottle, preserves under the conditions of-20 DEG C.
The preparation of the most anti-piglet isospora antibody
(body weight 4-8kg is made a definite diagnosis through spectroscopy and is uninfected by piglet coccidiosis to choose sodium selenite 5
Sick), the 1st, 7,21d carry out muscle with isospora antigen by 1.0mL/ head respectively
Injecting immune.After recording more than antibody titer 1:64, start to gather piglet serum,
Antibody is slightly carried, and through chromatography purification, with the dilution of 0.01mol/L PBS liquid and in-20 DEG C
Under the conditions of save backup.
3. the preparation of colloidal gold solution
First by 0.01% the aqueous solution 100mL of gold chloride (HAuCl4), under boil condition with
The trisodium citrate aqueous solution 2.5mL of 1%, it is orange red for continuing to boil 5min to solution, cold
But.Then adjust pH to 9.0 with 0.2mol/L K2CO3 solution, make gold colloidal colloidal sol.
4. the labelling of gold colloidal
Accurately weigh gold colloidal 20mL, add 100uL piglet isospora antigen, after mixing,
Stirring 20min, 4 DEG C stand 30min, add people bovine serum albumin 220mg, continue stirring
5min, 4 DEG C of 3000r/min are centrifuged 20min, discard precipitation, and 12000r/min is centrifuged 30min,
Precipitate 0.01mol/L, pH value is the PBS liquid 3.0mL dissolving of 7.4, obtains golden
Labeling antibody complex, 4 DEG C save backup.
5. the preparation of gold mark pad
Gold labeling antibody complex is spread evenly across on polyester fiber film, 37 DEG C of drying, standby.
6. detection line and the preparation of quality inspection line
Detection line is produced in nitrocellulose filter by antigen coated for anti-piglet isospora, will be anti-
Piglet isospora antibody is coated in nitrocellulose filter and produces quality inspection line, and 37 DEG C of drying are standby
With.
7. test strips assembles
Test strips is by sample pad 3, gold-marking binding pad 4, NC film 2, absorbent filter 7 four part
Composition.Sample pad 3, gold mark pad 4, NC film 2 and absorbent paper 7 are pasted on NC film 2 successively
On, detection line 5, quality inspection line 6 are pasted on PVC backer board 1, and quality inspection line 6 is away from gold mark
Pad 4, cuts into 0.4cm × 8.0cm bar, saves backup after drying.
During detection, the sample pad 3 (at sample-adding) in test strips drips 2～3 sample liquid.2～
Observed result in 5min, after 10min, observed result is invalid.Criterion: detection line 5 (T
Band), quality inspection line 6 (C band) all occur redness be the positive；Only quality inspection line 6 (C band) occurs red
Color for feminine gender: detection line 5 (T band), quality inspection line 6 (C band) all do not occur redness then reagent paper without
Effect.This utility model simple in construction, easy to operate, with low cost, quickly, diagnosis is accurate in detection
Really.It is particularly suited for the detection of piglet isospora.
Certainly, this utility model also can have other various embodiments, without departing substantially from this utility model
In the case of spirit and essence thereof, those of ordinary skill in the art can make according to this utility model
Go out various corresponding change and deformation, but these change accordingly and deformation all should belong to this practicality
Novel appended scope of the claims.
Claims (6)
1. a Test paper, it is characterised in that: include PVC backer board (1), and successively
The sample pad (3), the gold mark that are connected and be arranged on PVC backer board (1) pad (4), NC film
(2) and absorbent filter (7), described NC film (2) upper surface be provided with detection line (5) and
Quality inspection line (6), has gap, described quality inspection line (6) between detection line (5) and quality inspection line (6)
Away from gold mark pad (4).
Test paper the most according to claim 1, it is characterised in that: described detection line (5)
Including anti-piglet isospora antigen layer, described quality inspection line (6) includes anti-piglet isospora
Antibody layer, described anti-piglet isospora antigen layer and anti-piglet isospora antibody layer are attached to
On NC film (2).
Test paper the most according to claim 1 and 2, it is characterised in that: described gold mark
Pad (4) includes gold labeling antibody composite layer (42) and polyester fiber film (41), described polyester
Fibrous membrane (41) is attached on PVC backer board (1), described gold labeling antibody composite layer (42)
It is attached on polyester fiber film on (41).
Test paper the most according to claim 1, it is characterised in that: described sample pad (3),
Gold mark pad (4), NC film (2) and absorbent filter (7) are bonding with PVC backer board (1).
Test paper the most according to claim 1, it is characterised in that: described sample pad (3),
Gold mark pad (4), detection line (5), quality inspection line (6) and absorbent filter (7) are arranged in one
On straight line.
Test paper the most according to claim 1 or 5, it is characterised in that: described PVC
The size of backer board (1) is 0.4cm × 8.0cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620218241.8U CN205594013U (en) | 2016-03-18 | 2016-03-18 | Detection test paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620218241.8U CN205594013U (en) | 2016-03-18 | 2016-03-18 | Detection test paper |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205594013U true CN205594013U (en) | 2016-09-21 |
Family
ID=56928787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620218241.8U Expired - Fee Related CN205594013U (en) | 2016-03-18 | 2016-03-18 | Detection test paper |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205594013U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771180A (en) * | 2016-11-23 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | A kind of test strips for detecting Goose Parvovirus antibody |
-
2016
- 2016-03-18 CN CN201620218241.8U patent/CN205594013U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771180A (en) * | 2016-11-23 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | A kind of test strips for detecting Goose Parvovirus antibody |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160921 Termination date: 20190318 |