CN203350261U - Test paper card for quickly detecting dexamethasone - Google Patents
Test paper card for quickly detecting dexamethasone Download PDFInfo
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- CN203350261U CN203350261U CN 201320356842 CN201320356842U CN203350261U CN 203350261 U CN203350261 U CN 203350261U CN 201320356842 CN201320356842 CN 201320356842 CN 201320356842 U CN201320356842 U CN 201320356842U CN 203350261 U CN203350261 U CN 203350261U
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Abstract
The utility model relates to a test paper card for quickly detecting a dexamethasone (DEX) residue, which is characterized by comprising a plastic housing and a test paper strip packaged in the plastic housing, wherein a support layer is arranged on a bottom layer of the test paper strip; a sample absorption pad, a gold-labelled antibody binder release pad, a nitrocellulose membrane and a water absorption pad which are tightly connected from left to right sequentially are attached on the support layer; a detecting hole and a control hole are formed in the plastic housing; a position of the detecting hole corresponds to that of the sample absorption pad on the test paper strip; a position of the control hole corresponds to that of the nitrocellulose membrane on the test paper strip; the nitrocellulose membrane is wrapped with a detecting line of a dexamethasone coupling antigen and a quality control line of goat-anti-mouse IgG (immunoglobulin G); and the gold-labelled antibody binder release pad is coated with a dexamethasone monoclonal antibody labelled by colloidal gold. The test paper card is high in specificity, good in accuracy and high in detecting speed, and special instrument equipment or a professional operator is not required.
Description
Technical field
The utility model belongs to immunochemistry detection technique field, is specifically related to the residual quick detection test paper card of a kind of dexamethasone.
Background technology
Dexamethasone is a kind of adrenal cortex hormones drug, and its pharmacological action mainly contains the effects such as anti-inflammatory, anti-shock, antiallergy, antitoxin and immunosupress.Simultaneously again because it has the effect of significantly bringing down a fever, so some animal and veterinaries are widely used it as conventional antipyretic clinically.In recent years, the normal function of the aspects such as the edible beef that contains the hormones such as female enediol, progesterone, Medroxyprogesterone can be upset human endocrine, grows, immune system, reproductive system is thought by European Union, also may carcinogenic, teratogenesis, thereby forbid the use of this parahormone in animal.
Because dexamethasone is residual in animal viscera, the people, after the animal foodstuff of edible high residue, spinoff very easily occurs, shows as the hormone in vivo parasecretion, immunity of organisms is low, muscular atrophy is unable, the loose and growth retardation of sclerotin etc., but also the fever that can rise, weak and feeble, spirit is depressed, poor appetite, the symptoms such as blood sugar and blood pressure drops.Therefore, the most countries that comprises China all forbids that by dexamethasone, for Production of Livestock and Poultry, especially in recent years, the illegal use of dexamethasone is on the rise.
The residual detection method commonly used of dexamethasone has two kinds at present, a kind of is that red, orange, green, blue, yellow (ROGBY) is as high performance liquid chromatography (HPLC), vapor-phase chromatography (GC), gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS) etc., because its complicated instrument and equipment, expensive testing cost and the loaded down with trivial details inapplicable field monitoring of trace routine and a large amount of sample examinations.Another kind is immunological method, and as enzyme linked immunosorbent assay (ELISA), its shortcoming is that detection time is long, and testing cost is higher, is unfavorable for promoting the use of in grass-roots unit.
The purpose of this utility model is to provide a kind of easy to be quick for above-mentioned deficiency, and cost is low,, without training, be very suitable for the product of Site Detection.Compare with ELISA, there is sample pre-treatments simple, and all pack are contained on a test strips, without using the advantage such as instrument, there is wide market outlook and using value.Therefore, develop the residual quick detection test paper card of quick, sensitive, efficient dexamethasone for ensureing that the animal food safety tool is of great significance.
Summary of the invention
The technical matters that the utility model solves be to provide a kind of simple to operate, quick and precisely, highly sensitive, cost is low, good stability, can carry out the dexamethasone residue detection test card of batch detection.
Test card of the present utility model comprises plastic casing and is packaged in the test strips in plastic casing, the bottom of described test strips is supporting layer, being attached to has a closely connected absorption of sample pad successively by left-to-right on supporting layer, gold labeling antibody bond discharges pad, nitrocellulose filter and adsorptive pads, have on described plastic casing and detect hole and control wells, this position of detecting hole is corresponding with the absorption of sample pad position on test strips, control wells is corresponding with the cellulose nitrate film location on test strips, be surrounded by the detection line and the nature controlling line that is surrounded by sheep anti-mouse igg of dexamethasone (DEX) coupled antigen on described nitrocellulose filter, described golden labeling antibody bond discharges the dexamethasone monoclonal antibody that pad is coated with colloid gold label.
Supporting layer described in the utility model is to be coordinated the PVC plate made from stabilizing agent and auxiliary material by Corvic.Absorption of sample pad described in the utility model is that the plant long fibre extremely strong by water-intake capacity is the pure white filter paper that starting material are made.Golden labeling antibody bond described in the utility model discharges pad and is made by glass fibre cotton.Detection described in the utility model is the conjugates that dexamethasone and protein material adopt succinic anhydride method and carbodlimide method to form with dexamethasone even summation antigen.Protein substance described in the utility model is bovine serum albumin(BSA) or oralbumin.Sample pad described in the utility model, golden labeling antibody bond discharge pad and the adsorptive pads top is coated with rubber protective layer.
The utlity model has following advantage:
1. highly sensitive, high specificity.The residual quick detection test paper card of dexamethasone is prepared from the monoclonal antibody of colloid gold label high-affinity, in the gold labeling antibody, gold grain combines by Van der Waals force between the charges of different polarity with antibody molecule, and collaurum is very little on specificity and the affinity impact of monoclonal antibody.Therefore, the quick detection test paper jig has higher sensitivity and specificity, and its detection sensitivity can reach 5 ng/ml.
2. easy and simple to handle, fast.Without other any reagent, only the sample solution after processing need be splashed into and detects in hole 3 (about 90ml) with dropper while using the quick detection test paper card, 3-5 min gets final product observations.
3. testing result is vivid, directly perceived, accurate.Test card is usingd red trace line " | " or " ‖ " positive and the negative marker as detection line, when only nature controlling line (C line) shows a redness " | " trace on nitrocellulose filter, mean dexamethasone residual content >=5 ng/ml in detected sample solution; When if two redness " ‖ " trace appears in nature controlling line on nitrocellulose filter (C line) and detection line (T line) simultaneously, mean dexamethasone residual content<5 ng/ml in sample solution.Result is visual in image, accurately simple, is not easy to occur erroneous judgement.
4. low, the small investment of cost.Use the utility model test card, without complicated instrument and equipment, Site Detection settles at one go, with low cost, instant effect.
5. be easy to promote the use of on a large scale.This test card is simple to operate, without the professional technique requirement, can meet different levels personnel's detection needs, can be used for, as Entry-Exit Inspection and Quarantine Bureau, animal health supervision institute, quality testing department, breeding scale factory and animal product processing etc., thering is wide market outlook and larger economic benefit and social benefit.
The accompanying drawing explanation
Fig. 1 is the internal structure schematic diagram of dexamethasone test card in the utility model, wherein: 1, absorption of sample pad, 2, golden labeling antibody bond discharges pad, 3, nitrocellulose filter, 4, detection line, 5, nature controlling line, 6, adsorptive pads 7, pvc plate.
Fig. 2 is the outside drawing of test strips in the utility model: 8, plastic casing, 9, well 10, view window.
Fig. 3 be test strips in the utility model the reaction schematic diagram wherein: the positive Fig. 3 c of the negative Fig. 3 b of Fig. 3 a is invalid notes: C for accusing line, and T is detection line.
Embodiment
Describe by reference to the accompanying drawings embodiment in detail.The residual quick detection test paper card of a kind of dexamethasone, absorption of sample pad 1 is to be the pure white filter paper that starting material are made by the plant long fibre.Gold labeling antibody bond discharges pad 2 for adsorbing the fibrage of the dexamethasone monoclonal antibody that colloid gold label is arranged; Reaction film 3 is nitrocellulose filter; Detectability 4 is for being coated with dexamethasone-carrier protein couplet thing; Nature controlling line 5 is for being coated with sheep anti-mouse igg; Adsorptive pads 6 is made for absorbent material; PVC7 is that supporting layer is made by plastic tab.Discharge pad 2 nitrocellulose filters 3 and adsorptive pads 6 by the left-to-right closely connected absorption of sample pad 1 gold medal labeling antibody bond that is attached with successively on pvc, have well 9 and view window 10 on described plastic casing 8, the position of this well 9 is corresponding with absorption of sample pad 1 position on test card, view window 10 is corresponding with nitrocellulose filter 3 positions on test card, detection line (the T line) 4 of dexamethasone-carrier protein couplet thing printing and the nature controlling line (C line) 5 that sheep anti-mouse igg solution is printed are arranged, between the two apart from 6mm on described nitrocellulose filter 3.
The using method of test card of the present utility model:
1, sample pre-treatments:
Take tissue sample after 10g homogenate in centrifuge tube, add the methanol solution of 20ml70%, after vibration mixes 3min, the sample supernatant of getting after processing is standby.
2, with test card, detect:
After test card is kept flat, with dropper, sample solution is splashed in the test card well to 3, get final product after 3-5 min
Observations.
3, Analysis of test results:
It is negative: if, when on nitrocellulose filter, two redness " ‖ " trace appears in nature controlling line (C line) and detection line (T line), dexamethasone residual content<5 ng/ml in the expression sample solution, be judged to feminine gender, as shown in Fig. 3 a simultaneously.
Positive: if on nitrocellulose filter, nature controlling line (C line) shows a redness " | " trace, and detection line (T line) does not develop the color, and means dexamethasone residual content >=5 ng/ml in detected sample solution, is judged to the positive, as shown in Fig. 3 b.
Invalid: if on nitrocellulose filter, nature controlling line (C line) does not show red stripes, no matter whether detection line (T line) red trace occurs, and it is invalid that this test card all is judged to, as shown in Fig. 3 c.
Claims (4)
1. the residual quick detection test paper card of dexamethasone, it is characterized in that: the residual quick detection test paper card of described dexamethasone comprises plastic casing and is packaged in the test strips in plastic casing, the bottom of described test strips is supporting layer, being attached to has a closely connected absorption of sample pad successively by left-to-right on supporting layer, gold labeling antibody bond discharges pad, nitrocellulose filter and adsorptive pads, have on described plastic casing and detect hole and control wells, this position of detecting hole is corresponding with the absorption of sample pad position on test strips, the position of control wells is corresponding with the cellulose nitrate film location on test strips, be surrounded by the detection line and the nature controlling line that is surrounded by sheep anti-mouse igg of dexamethasone coupled antigen on described nitrocellulose filter, described golden labeling antibody bond discharges the dexamethasone monoclonal antibody that pad is coated with colloid gold label.
2. the residual quick detection test paper card of dexamethasone according to claim 1, it is characterized in that: described absorption of sample pad is to be the pure white filter paper that starting material are made by the plant long fibre.
3. the residual quick detection test paper card of dexamethasone according to claim 1 is characterized in that: the distance between described detection line and nature controlling line is 6mm.
4. the residual quick detection test paper card of dexamethasone according to claim 1, is characterized in that: above described absorption of sample pad, golden labeling antibody bond release pad and adsorptive pads, be coated with the glued membrane protection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320356842 CN203350261U (en) | 2013-06-20 | 2013-06-20 | Test paper card for quickly detecting dexamethasone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320356842 CN203350261U (en) | 2013-06-20 | 2013-06-20 | Test paper card for quickly detecting dexamethasone |
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| Publication Number | Publication Date |
|---|---|
| CN203350261U true CN203350261U (en) | 2013-12-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201320356842 Expired - Fee Related CN203350261U (en) | 2013-06-20 | 2013-06-20 | Test paper card for quickly detecting dexamethasone |
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| CN (1) | CN203350261U (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105044367A (en) * | 2015-08-03 | 2015-11-11 | 武汉上成生物科技有限公司 | Colloidal gold immunochromatographic assay test strip for detecting dexamethasone residues in milk |
| CN105838681A (en) * | 2016-05-30 | 2016-08-10 | 江南大学 | Anti-dexamethasone-specificity monoclonal antibody hybridoma cell strain C3 and application thereof |
| CN106317153A (en) * | 2015-06-18 | 2017-01-11 | 北京维德维康生物技术有限公司 | Preparation method and application of dexamethasone semiantigen |
| CN106771007A (en) * | 2016-12-07 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | The detection method and test strips of dexamethasone in a kind of dairy products |
| CN109030838A (en) * | 2018-10-11 | 2018-12-18 | 北京工商大学 | It is a kind of for detecting the colloid gold test paper of dexamethasone |
| CN112362881A (en) * | 2020-11-09 | 2021-02-12 | 华南农业大学 | Immune latex microsphere micropore detection system for rapidly detecting dexamethasone and preparation method and application thereof |
-
2013
- 2013-06-20 CN CN 201320356842 patent/CN203350261U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106317153A (en) * | 2015-06-18 | 2017-01-11 | 北京维德维康生物技术有限公司 | Preparation method and application of dexamethasone semiantigen |
| CN106317153B (en) * | 2015-06-18 | 2019-10-11 | 北京维德维康生物技术有限公司 | A kind of dexamethasone haptens preparation method and applications |
| CN105044367A (en) * | 2015-08-03 | 2015-11-11 | 武汉上成生物科技有限公司 | Colloidal gold immunochromatographic assay test strip for detecting dexamethasone residues in milk |
| CN105838681A (en) * | 2016-05-30 | 2016-08-10 | 江南大学 | Anti-dexamethasone-specificity monoclonal antibody hybridoma cell strain C3 and application thereof |
| CN105838681B (en) * | 2016-05-30 | 2019-04-30 | 江南大学 | One plant of anti-dexamethasone monoclonal antibody specific hybridoma cell strain C3 and its application |
| CN106771007A (en) * | 2016-12-07 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | The detection method and test strips of dexamethasone in a kind of dairy products |
| CN109030838A (en) * | 2018-10-11 | 2018-12-18 | 北京工商大学 | It is a kind of for detecting the colloid gold test paper of dexamethasone |
| CN112362881A (en) * | 2020-11-09 | 2021-02-12 | 华南农业大学 | Immune latex microsphere micropore detection system for rapidly detecting dexamethasone and preparation method and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131218 Termination date: 20140620 |
|
| EXPY | Termination of patent right or utility model |