CN201489000U - Colloidal gold test paper card for detecting clenbuterol drug residue - Google Patents
Colloidal gold test paper card for detecting clenbuterol drug residue Download PDFInfo
- Publication number
- CN201489000U CN201489000U CN2009201085444U CN200920108544U CN201489000U CN 201489000 U CN201489000 U CN 201489000U CN 2009201085444 U CN2009201085444 U CN 2009201085444U CN 200920108544 U CN200920108544 U CN 200920108544U CN 201489000 U CN201489000 U CN 201489000U
- Authority
- CN
- China
- Prior art keywords
- clenbuterol
- test card
- mat
- test paper
- bond
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000012360 testing method Methods 0.000 title claims abstract description 48
- STJMRWALKKWQGH-UHFFFAOYSA-N clenbuterol Chemical compound CC(C)(C)NCC(O)C1=CC(Cl)=C(N)C(Cl)=C1 STJMRWALKKWQGH-UHFFFAOYSA-N 0.000 title claims abstract description 22
- 229960001117 clenbuterol Drugs 0.000 title claims abstract description 22
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 9
- 239000003640 drug residue Substances 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 claims abstract description 36
- 238000006243 chemical reaction Methods 0.000 claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 4
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 4
- 239000002250 absorbent Substances 0.000 claims description 14
- 230000002745 absorbent Effects 0.000 claims description 14
- 239000000020 Nitrocellulose Substances 0.000 claims description 5
- 230000000274 adsorptive effect Effects 0.000 claims description 5
- 229920001220 nitrocellulos Polymers 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 241001494479 Pecora Species 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 239000011491 glass wool Substances 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 2
- 229920000728 polyester Polymers 0.000 claims description 2
- 239000012528 membrane Substances 0.000 abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 210000002700 urine Anatomy 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract 5
- 239000010931 gold Substances 0.000 description 5
- 229910052737 gold Inorganic materials 0.000 description 5
- 238000003908 quality control method Methods 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 239000000084 colloidal system Substances 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000002860 competitive effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 206010046607 Urine abnormality Diseases 0.000 description 1
- 238000005411 Van der Waals force Methods 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000003255 drug test Methods 0.000 description 1
- 108060003552 hemocyanin Proteins 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 235000020997 lean meat Nutrition 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 238000003822 preparative gas chromatography Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000011287 therapeutic dose Methods 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- 238000003805 vibration mixing Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Abstract
The utility model provides a clenbuterol detection test paper card which comprises a plastic box body and a test paper strip arranged in the plastic box body, wherein the test paper strip comprises a bottom plate, a sample absorption mat, a conjugate releasing mat, a reaction membrane and a water absorption mat, wherein the sample absorption mat, the conjugate releasing mat, the reaction membrane and the water absorption mat are attached on the bottom plate and are sequentially connected tightly; and the sample absorption mat covers a region from 1/2-1/3 of the conjugate releasing mat. The reaction membrane is provided with a detection line enveloping clenbuterol-carrier protein conjugate and a composition control line enveloping goat-anti-mouse IgG; and the conjugate releasing mat envelopes a clenbuterol antibody marked by colloidal gold. The test paper card can play an important role in the detection of the clenbuterol drug residue in tissue and urine samples, and has the characteristics of good probability, high sensitivity, little error, short detection time and the like.
Description
Technical field
The utility model belongs to the immunochemistry speed survey technology field of residue of veterinary drug, is specifically related to a kind of Clenbuterol drug test test card.
Background technology
Clenbuterol (Clenbuterol) belongs to beta-stimulants, illegally added to lean meat percentage and the acceleration growth of animal to improve the lard type animal in the feed in recent years, because of its additive capacity be therapeutic dose 5-10 doubly, to such an extent as to residual quantity is high and bring harm to the consumer in animal body.
The detection method that residual of kelengtelu is commonly used has two kinds at present.A kind of is stratographic analysis, as vapor-phase chromatography (GC), because complicated instrument and equipment and loaded down with trivial details process are not suitable for on-site supervision and great amount of samples examination.Another kind is an immunological method, as enzyme linked immunosorbent assay (ELISA).Its shortcoming is that detection time is long, and expense is higher, is unfavorable for promoting the use of in grass-roots unit.And these two kinds of methods all need the professional and technical personnel to operate.
The utility model content
The purpose of this utility model provides a kind of highly sensitive, cost is low, simple to operate, detection time is short test card.
Test card of the present utility model comprises the test strips in plastic box body and the box body, have well and view window on the plastic box body, test strips is by base plate, attached to the sample absorbent that closely links to each other successively on the base plate, bond discharges pad, composition such as reaction film and adsorptive pads, different with conventional test strips is, it is not to cover under the sample absorbent fully that bond wherein discharges pad, but the subregion is covered under the sample absorbent, / 2nd to 1/3rd zones that bond discharges pad are covered under the sample absorbent, and 1/3rd zones that the preferred combination thing discharges pad are covered under the sample absorbent.
The benefit of doing like this is to prolong testing result observing time, and sample absorbent also can fully absorb tracer liquid and the complete full contact reaction of golden labeling antibody, and chromatography reacts to reaction film again, can effectively reduce error.Can also prevent to detect that some interfering components lose activity the albumen in the golden labeling antibody in the sample, influence combining of golden labeling antibody and coating antigen.
Be coated with the detection line trace " | " of Clenbuterol-carrier protein couplet thing formation and the nature controlling line trace " | " that bag is constituted by sheep anti-mouse igg on the described reaction film respectively, detection line is positioned at apart from bond and discharges pad one side 0.5cm place, and detection line is parallel with nature controlling line.Thereby the residue detection that can be used for the Clenbuterol medicine.
Have well and view window on the utility model test card plastic box body, but the material that the base plate of test strips does not absorb water for PVC plate or other hard in the box body; Reaction film can be nitrocellulose filter or cellulose acetate membrane; Sample absorbent can be made by glass wool or polyester material; Bond discharges pad can be made by fiber, and bond discharges bed course and is coated with antibody of clenbuteral-collaurum bond.
Coupling Clenbuterol carrier protein can be with ovoserum albumen or human serum albumins, bovine serum albumin(BSA), hemocyanin etc., and the contrast trace assembled arrangement of detection line trace on reaction film and nature controlling line can be " || "
The utility model test card has following beneficial effect:
1. high specificity is highly sensitive.Clenbuterol quick detection test paper card is that the basis is prepared from the monoclonal of colloid gold label high-affinity, priceless strong formation between gold grain and the antibody molecule in the gold labeling antibody, the two combines by the Van der Waals force between the charges of different polarity, collaurum is very little to monoclonal specificity and affinity influence, and has higher mark rate.Therefore, the quick detection test paper jig has higher specificity and sensitivity.The test card detection sensitivity can reach 5 μ g/ml (μ g/g).
2. easy and simple to handle quick.Need not any other reagent when using the quick detection test paper card,, drip 3 (about 100 microlitres), timing behind the application of sample, observations in 5～8min as long as sample is splashed in the reagent hole clipping with dropper.
3. show testing result image, accurately directly perceived.The quick detection test paper card is to show red line look " | " and " || " trace positive and negative marker as detection line, show on nitrocellulose filter that promptly a red line " | " trace is illustrated in the content of Clenbuterol in the detected sample liquid more than or equal to 5 μ g/ml (μ g/g), article two, red line " || " trace is illustrated in that Clenbuterol content is lower than 5 μ g/ml (μ g/g) in the detected sample, the result judges image, directly perceived, accurate, simple and clear, is not easy to occur result's erroneous judgement.
4. cost is low, small investment.Use the quick detection test paper bar, do not need to join in addition instrument and equipment and other reagent, the on-the-spot detection settled at one go, with low cost, small investment, instant effect.
5. be easy to apply on a large scale.The quick detection test paper card is simple to operate, can better satisfy different levels personnel's needs, arrive individual breed etc. as specialty chemical examination, customs quarantine control, health and epidemic prevention, quality monitoring, animal product processing, collectionization breed, have vast market prospect and bigger economical, societal benefits.
Description of drawings
Fig. 1 is a Clenbuterol test strip structural representation, wherein, and 1, sample absorbent; 2, bond discharges pad; 3, reaction film; 4, adsorptive pads; 5, detection line; 6, nature controlling line; 7, base plate;
Fig. 2 is that Clenbuterol detects test card plan structure synoptic diagram, wherein, and 8, plastic box body; 9, well; 10, view window;
Fig. 3 is Clenbuterol quick detection test paper card feminine gender (Fig. 3 .a), positive (Fig. 3 .b), invalid (Fig. 3 .c) synoptic diagram that develops the color, T detection line wherein, and C is a nature controlling line.
Embodiment
Be described further below in conjunction with accompanying drawing
1, Clenbuterol detects test card:
Sample absorbent (1) is made by glass wool, it is the fibrage that is adsorbed with the Clenbuterol monoclonal antibody of colloid gold label that bond discharges pad (2), reaction film (3) is a nitrocellulose filter, adsorptive pads (4) is made for absorbent material layer, detection line (5) is coated with Clenbuterol-carrier protein couplet thing, nature controlling line (6) is coated with sheep anti-mouse igg, PVC backing (7) is made by the plastic tab bar for supporting layer, on PVC backing (7), adhere to nitrocellulose filter (3) in order, adsorptive pads (4), bond discharges pad (2) and sample pad (1), sample pad is covered bond discharge pad 1/3rd places, form test strips and be packaged in the special plastic box body (8) that has well (9) and view window (10).
2, the use of test card
(1) sample pre-treatment:
Urine
Limpid urine can directly be got 100 μ l point samples, and as cloudy urine, the centrifugal 5min of the above room temperature of 3000g gets supernatant 100 μ l point samples.
Pork
Take by weighing and organize 2g in centrifuge tube after the homogenate, add 1ml water vibration mixing, 10min in 80 ℃ of water-baths takes out and is cooled to room temperature, gets supernatant 100 μ l point samples.
(2) detect with test card
Sample is splashed in the reagent hole clipping with dropper, drip 3, timing behind the application of sample, observations in 5～8min.
3, testing result analysis
When Clenbuterol concentration in sample was higher than 5 μ g/ml (μ g/g), the antibody of clenbuteral of colloid gold label combined with the Clenbuterol medicine is whole, thereby did not occur red stripes in detection zone because competitive reaction can not combine with the Clenbuterol coupled antigen.Negative sample owing to lack the antibody antigen competitive reaction, will red stripes occur in detection zone and Quality Control district in testing process.
Positive: when red stripes of Quality Control district demonstration, and detection zone does not develop the color, and the quick detection test paper card is judged to the positive, shown in Fig. 3 .b figure to show red line look " | ".
Negative: when the Quality Control district shows a red stripes, detection zone also demonstrates a red stripes simultaneously, and the detection zone color near or when being shallower than the Quality Control district, the quick detection test paper card is judged to feminine gender, shown in Fig. 3 .a to show that the red line look is " || ".
Invalid: when the Quality Control district does not demonstrate red stripes, then no matter whether detection zone demonstrates red stripes, and it is invalid that this test card is judged to, shown in Fig. 3 .c.
Claims (9)
1. colloidal gold test paper card that detects the Clenbuterol medicament residue, it is characterized in that test card comprises the test strips in plastic box body and the box body, test strips is by base plate, attached to the sample absorbent that closely links to each other successively on the base plate, bond release pad, reaction film and adsorptive pads, and 1/2nd to 1/3rd zones that bond discharges pad are covered under the sample absorbent.
2. test card as claimed in claim 1 is characterized in that having on the plastic box body well and view window.
3. test card as claimed in claim 1 is characterized in that bond discharges 1/3rd zones of filling up and is covered under the sample absorbent.
4. test card as claimed in claim 1 is characterized in that having on the described reaction film detection line that is coated with Clenbuterol-carrier protein couplet thing formation and the nature controlling line that bag is constituted by sheep anti-mouse igg.
5. test card as claimed in claim 4 is characterized in that described detection line is parallel with nature controlling line.
6. test card as claimed in claim 4 is characterized in that detection line is positioned at apart from bond release pad one side 0.5cm place.
7. test card as claimed in claim 1 or 2 is characterized in that described base plate is the PVC plate, and described reaction film is a nitrocellulose filter.
8. test card as claimed in claim 1 or 2 is characterized in that described sample absorbent made by glass wool or polyester material, and described bond is released bed course and made by fiber.
9. test card as claimed in claim 1 or 2 is characterized in that described bond discharges pad for being coated with antibody of clenbuteral-collaurum bond.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201085444U CN201489000U (en) | 2009-06-01 | 2009-06-01 | Colloidal gold test paper card for detecting clenbuterol drug residue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201085444U CN201489000U (en) | 2009-06-01 | 2009-06-01 | Colloidal gold test paper card for detecting clenbuterol drug residue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201489000U true CN201489000U (en) | 2010-05-26 |
Family
ID=42427873
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009201085444U Expired - Lifetime CN201489000U (en) | 2009-06-01 | 2009-06-01 | Colloidal gold test paper card for detecting clenbuterol drug residue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201489000U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104090248A (en) * | 2013-12-24 | 2014-10-08 | 上海容晖生物科技有限公司 | Reagent for quantitative detection of Beta-receptor stimulant through Europium chelate latex time-resolved immunochromatographic assay |
| CN106771166A (en) * | 2016-12-30 | 2017-05-31 | 天津灵卫科技发展有限公司 | A kind of portable clenbuterol hydrochloride assay kit |
| CN106771167A (en) * | 2016-12-30 | 2017-05-31 | 天津灵卫科技发展有限公司 | A kind of clenbuterol hydrochloride assay kit |
-
2009
- 2009-06-01 CN CN2009201085444U patent/CN201489000U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104090248A (en) * | 2013-12-24 | 2014-10-08 | 上海容晖生物科技有限公司 | Reagent for quantitative detection of Beta-receptor stimulant through Europium chelate latex time-resolved immunochromatographic assay |
| CN106771166A (en) * | 2016-12-30 | 2017-05-31 | 天津灵卫科技发展有限公司 | A kind of portable clenbuterol hydrochloride assay kit |
| CN106771167A (en) * | 2016-12-30 | 2017-05-31 | 天津灵卫科技发展有限公司 | A kind of clenbuterol hydrochloride assay kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: BEIJING WANGER BIOTECHNOLOGY CO., LTD. |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 102206 NO. 8, GAOXIN STREET 4, INTERNATIONAL INFORMATION INDUSTRIAL BASE, HUILONGGUAN TOWN, CHANGPING DISTRICT, BEIJING TO: 102206 NO. 8, GAOXIN STREET 4, INTERNATIONAL INFORMATION INDUSTRIAL BASE, HUILONGGUAN, CHANGPING DISTRICT, BEIJING |
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| TR01 | Transfer of patent right |
Effective date of registration: 20110113 Address after: 102206 Huilongguan international information industry base, Changping District, high street, No. 8, No. four, Beijing Patentee after: Beijing Wanger Biotechnology Co., Ltd. Address before: 102206 Beijing city Changping District Huilongguan international information industry base, four High Street No. 8 Co-patentee before: Beijing Wanger Biotechnology Co., Ltd. Patentee before: Beijing Wanger Biotechnology Co., Ltd. |
|
| ASS | Succession or assignment of patent right |
Owner name: BEIJING KWINBON BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: BEIJING WANGER BIOTECHNOLOGY CO., LTD. Effective date: 20120507 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20120507 Address after: 102206 Huilongguan international information industry base, Changping District, high street, No. 8, No. four, Beijing Patentee after: Beijing Kwinbon Biotechnology Co., Ltd. Address before: 102206 Huilongguan international information industry base, Changping District, high street, No. 8, No. four, Beijing Patentee before: Beijing Wanger Biotechnology Co., Ltd. |
|
| ASS | Succession or assignment of patent right |
Owner name: GUIZHOU QINBANG FOOD SAFETY SCIENCE AND TECHNOLOGY Free format text: FORMER OWNER: BEIJING KWINBON BIOTECHNOLOGY CO., LTD. Effective date: 20130531 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 102206 CHANGPING, BEIJING TO: 550009 GUIYANG, GUIZHOU PROVINCE |
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| TR01 | Transfer of patent right |
Effective date of registration: 20130531 Address after: 550009, Guizhou province Guiyang Xiaohe Industrial Park standard factory building two, 1, building 1-2 Patentee after: GUIZHOU QINBANG FOOD SAFETY SCIENCE AND TECHNOLOGY CO., LTD. Address before: 102206 Huilongguan international information industry base, Changping District, high street, No. 8, No. four, Beijing Patentee before: Beijing Kwinbon Biotechnology Co., Ltd. |
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| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20100526 |
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| DD01 | Delivery of document by public notice |
Addressee: GUIZHOU QINBANG FOOD SAFETY SCIENCE AND TECHNOLOGY CO., LTD. Document name: Notification of Expiration of Patent Right Duration |
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| DD01 | Delivery of document by public notice |