CN106771167A - A kind of clenbuterol hydrochloride assay kit - Google Patents
A kind of clenbuterol hydrochloride assay kit Download PDFInfo
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- CN106771167A CN106771167A CN201611251920.6A CN201611251920A CN106771167A CN 106771167 A CN106771167 A CN 106771167A CN 201611251920 A CN201611251920 A CN 201611251920A CN 106771167 A CN106771167 A CN 106771167A
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- hydrochloride
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- Urology & Nephrology (AREA)
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Abstract
The invention provides a kind of clenbuterol hydrochloride assay kit, lid including the connection of box body, one end and box body, box body inner chamber is provided with placement plate, place and offer several test card slots, dropper groove, reagent bottle groove and sample tube seat on plate, storage respectively has clenobuterol hydrochloride to detect card, dropper, reagent bottle and sample cell.The invention is full-featured, small volume, easy to carry, simple to operate, the quick detection at suitable scene.
Description
Technical field
The invention belongs to field of food detection, more particularly, to a kind of clenbuterol hydrochloride assay kit.
Background technology
Clenobuterol hydrochloride, also known as " clenbuterol hydrochloride ", is a kind of beta-2-adrenoreceptor agonist, is clinically often used in controlling
COPD (COPD) is treated, also by as the bronchodilator relaxed when panting acute attack
(Bronchodilator) medication, belongs to adrenal gland class CNS stimulant.Clenizole Hydrochloride can be obviously promoted growth of animal, and
Increase lean meat percentage, it can change the metabolic pathway in animal body, promote the synthesis of protein in muscle, particularly skeletal muscle,
Suppress the synthesis of fat, so as to accelerate the speed of growth, lean meat relative increase improves carcass quality.Hydrochloric acid gram is with the addition of in feed
After Lun Teluo, the growth of animals or poultry such as pig speed, feed conversion rate, carcass lean meat percentage can be made to improve more than 10%, with hydrochloric acid gram human relations
Application of special sieve in husbandry sector, it has been found that clenobuterol hydrochloride can in animal body accumulate residual, causes edible poisoning.
Clenobuterol hydrochloride poisoning can cause serious harm to the healthy of people, and its symptom includes：Blood pressure rising, blood vessel dilatation,
Palpitating speed, breathing aggravation, body temperature rising, muscle tremors, headache, uncomfortable in chest, oversensitive, myalgia, palpitaition, nausea, vomit
Tell.Because clenobuterol hydrochloride is serious as the harm of feed addictive, China has prohibited it and has been supported in food animal
Used in growing, but the situation of illegal abuse domestic at present is still serious.
At present, the detection method of clenobuterol hydrochloride mainly has liquid chromatography, liquid/matter combination analysis method, enzyme linked immunological
Absorption method etc..But these methods are often cumbersome, required expensive equipment, need professional operator or detecting step
It is many, high to detection environmental requirement, the quick detection at scene is not appropriate for, it is unfavorable for the popularization of basic unit.
The content of the invention
In view of this, the invention is directed to a kind of simple structure, simple to operate, small volume, salt easy to carry
Clenbuterol detection kit, to solve the above problems.
To reach above-mentioned purpose, what the technical scheme of the invention was realized in：
A kind of clenbuterol hydrochloride assay kit, including the lid that box body, one end are connected with the box body, the box body
Inner chamber is provided with placement plate, and several test card slots, dropper groove, reagent bottle groove and sample cell are offered on the placement plate
Groove, respectively storage has clenobuterol hydrochloride to detect card, dropper, reagent bottle and sample cell.
Further, the clenobuterol hydrochloride detection card includes cartridge and the hydrochloric acid Ke Lunte located at the cartridge inner chamber
Sieve detector bar；The cartridge includes base plate and fastens to be fixed with the cap on the base plate, the clenobuterol hydrochloride detector bar
In on the base plate；The clenobuterol hydrochloride detector bar includes the sample pad, the Jin Biao that are sequentially connected along sample liquid flow direction
Thing pad, reaction film and adsorptive pads；Detection line and nature controlling line are provided with the reaction film；With the sample on the cap
The corresponding position of product pad is provided with well, and the position corresponding with the reaction film is provided with observation window.
Further, it is coated with the detection line on clenobuterol hydrochloride-carrier protein couplet thing, the nature controlling line and is wrapped
There is sheep anti-mouse igg antibody, the resisting clenbuterol hydrochloride mouse antibody of colloid gold label is coated with the gold mark thing pad.
Further, box body inner chamber lower end is provided with foam pad, is offered on the foam pad and clenobuterol hydrochloride
Detection card, dropper, the reagent bottle low groove corresponding with sample bottom of the tube.
Relative to prior art, a kind of clenbuterol hydrochloride assay kit described in the invention has following excellent
Gesture：
It is the invention simple structure, full-featured, small volume, easy to carry, it is right using clenobuterol hydrochloride detection card
Sample detected, simple to operate, low cost, without professional's operation, and suitable ordinary people is used for quickly detecting at the scene；Box
Deposit multiple detection cards simultaneously in vivo, multigroup sample can be detected；It is rational in infrastructure that detection blocks, and sample liquid instills sample by well
On product pad, from observation window from the change of observation detection line and nature controlling line, easy to use, detection is quick；Box body inner-cavity structure is set
Rationally, it is ensured that the stabilization of each several part storage, it is to avoid clenobuterol hydrochloride detection card, dropper, reagent bottle and sample cell are due to collision
Generation is damaged.
Brief description of the drawings
The accompanying drawing for constituting the part of the invention is used for providing further understanding the invention, present invention wound
The schematic description and description made does not constitute the improper restriction to the invention for explaining the invention.
In accompanying drawing：
Fig. 1 is the structural representation of kit described in the invention；
Fig. 2 is the structural representation of clenobuterol hydrochloride detection card described in the invention；
Fig. 3 is the top view of clenobuterol hydrochloride detection card described in the invention.
Description of reference numerals：
1- box bodys；2- lids；3- places plate, 31- test card slots, 32- dropper grooves, 33- reagent bottle grooves, 34- sample tube seats；
4- cartridges, 41- base plates, 42- caps, 43- wells, 44- observation windows；5- clenobuterol hydrochloride detector bars, 51- sample pads, 52-
Gold mark thing pad, 53- reaction films, 54- adsorptive pads, 55- detection lines, 56- nature controlling lines.
Specific embodiment
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the invention can
To be mutually combined.
In the description of the invention, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " on ", D score,
The orientation or position relationship of the instruction such as "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outward " are
Based on orientation shown in the drawings or position relationship, it is for only for ease of description the invention and simplifies description, rather than instruction
Or imply that the device or element of meaning with specific orientation, with specific azimuth configuration and operation, therefore must not be understood that
It is the limitation to the invention.Additionally, term " first ", " second " etc. are only used for describing purpose, and it is not intended that indicating
Or imply relative importance or the implicit quantity for indicating indicated technical characteristic.Thus, " first ", " second " etc. are defined
Feature can express or implicitly include one or more this feature.In the description of the invention, unless separately
It is described, " multiple " is meant that two or more.
, it is necessary to explanation, unless otherwise clearly defined and limited, term " is pacified in the description of the invention
Dress ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected, or be detachably connected, or integratedly
Connection；Can mechanically connect, or electrically connect；Can be joined directly together, it is also possible to be indirectly connected to by intermediary,
Can be two connections of element internal.For the ordinary skill in the art, can be by concrete condition understanding
State concrete meaning of the term in the invention.
Describe the invention in detail below with reference to the accompanying drawings and in conjunction with the embodiments.
As shown in figure 1, a kind of clenbuterol hydrochloride assay kit, including box body 1, one end and the lid of the connection of box body 1
2, lid 2 can be covered on box body 1；The inner chamber of box body 1 is provided with placement plate 3, to place and offer three row's test card slots on plate 3
31, often arranging has multiple test card slots 31, dropper groove 32, reagent bottle groove 33 and sample tube seat 34, and storage respectively has hydrochloric acid gram human relations
Special sieve detection card, dropper, reagent bottle and sample cell；The inner chamber lower end of box body 1 is provided with foam pad, is offered on foam pad and hydrochloric acid gram
Lun Teluo detections card, dropper, the reagent bottle low groove corresponding with sample bottom of the tube, it is ensured that clenobuterol hydrochloride detection card, drop
The stabilization that pipe, reagent bottle and sample cell are placed, it is to avoid collide and the generation of the situation of breakage；
As shown in Figure 2 and Figure 3, clenobuterol hydrochloride detection card includes cartridge 4 and the hydrochloric acid Ke Lunte located at the inner chamber of cartridge 4
Sieve detector bar 5；Cartridge 4 includes base plate 41 and the cap 42 being fastened on base plate 41, and clenobuterol hydrochloride detector bar 5 is fixed on bottom
On plate 41；Clenobuterol hydrochloride detector bar 5 includes the sample pad 51, the gold mark thing pad that are sequentially connected along sample liquid flow direction
52nd, reaction film 53 and adsorptive pads 54；Detection line 55 and nature controlling line 56 are provided with reaction film 53；Salt is coated with detection line 55
Sheep anti-mouse igg antibody is coated with Clenbuterol-carrier protein couplet thing, nature controlling line 56, is coated with gold mark thing pad 52
There is the resisting clenbuterol hydrochloride mouse antibody of colloid gold label；The position corresponding with sample pad 51 is provided with well on cap 42
43, for the dropwise addition of sample liquid, the position corresponding with reaction film 53 is provided with observation window 44.
The use process of the invention is as follows：
Open the lid 2, taking-up clenobuterol hydrochloride detection card, with the sample liquid in dropper pipette samples pipe, through well
43 instill in sample pad 51, wait 2-5 minutes, the change of observation detection line 55 and nature controlling line 56, result of determination；
Testing result：If detection line 55 and nature controlling line 56 develop the color, then it represents that clenobuterol hydrochloride in sample contains
Amount is not exceeded；If detection line 55 does not develop the color, nature controlling line 56 develops the color, then it represents that the content of the clenobuterol hydrochloride in sample surpasses
Mark；If nature controlling line 56 does not develop the color, illustrate that operation is incorrect or clenobuterol hydrochloride test strip fails.
The preferred embodiment of the invention is the foregoing is only, creation is not intended to limit the invention, it is all at this
Within the spirit and principle of innovation and creation, any modification, equivalent substitution and improvements made etc. should be included in the invention
Protection domain within.
Claims (4)
1. the lid (2) that a kind of clenbuterol hydrochloride assay kit, including box body (1), one end are connected with the box body (1),
It is characterized in that：Box body (1) inner chamber is provided with placement plate (3), and several test card slots are offered on placement plate (3)
(31), dropper groove (32), reagent bottle groove (33) and sample tube seat (34), respectively storage have clenobuterol hydrochloride to detect card, drop
Pipe, reagent bottle and sample cell.
2. a kind of clenbuterol hydrochloride assay kit according to claim 1, it is characterised in that：The hydrochloric acid Ke Lunte
Sieve detection card includes cartridge (4) and the clenobuterol hydrochloride detector bar (5) located at the cartridge (4) inner chamber；Cartridge (4) bag
The cap (42) for including base plate (41) and being fastened on the base plate (41), the clenobuterol hydrochloride detector bar (5) is fixed on institute
State on base plate (41)；The clenobuterol hydrochloride detector bar (5) includes the sample pad that is sequentially connected along sample liquid flow direction
(51), gold mark thing pad (52), reaction film (53) and adsorptive pads (54)；Detection line is provided with the reaction film (53)
And nature controlling line (56) (55)；The position corresponding with the sample pad (51) is provided with well (43) on the cap (42), with
The corresponding position of the reaction film (53) is provided with observation window (44).
3. a kind of clenbuterol hydrochloride assay kit according to claim 2, it is characterised in that：The detection line (55)
On be coated with clenobuterol hydrochloride-carrier protein couplet thing, the nature controlling line and be coated with sheep anti-mouse igg antibody on (56), it is described
The resisting clenbuterol hydrochloride mouse antibody of colloid gold label is coated with gold mark thing pad (52).
4. a kind of clenbuterol hydrochloride assay kit according to claim 1, it is characterised in that：In the box body (1)
Chamber lower end is provided with foam pad, is offered on the foam pad and clenobuterol hydrochloride detection card, dropper, reagent bottle and sample cell bottom
The corresponding low groove in portion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611251920.6A CN106771167A (en) | 2016-12-30 | 2016-12-30 | A kind of clenbuterol hydrochloride assay kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611251920.6A CN106771167A (en) | 2016-12-30 | 2016-12-30 | A kind of clenbuterol hydrochloride assay kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106771167A true CN106771167A (en) | 2017-05-31 |
Family
ID=58927750
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611251920.6A Pending CN106771167A (en) | 2016-12-30 | 2016-12-30 | A kind of clenbuterol hydrochloride assay kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106771167A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389883A (en) * | 2017-08-10 | 2017-11-24 | 安徽优乐亿乳业有限公司 | A kind of multi-functional Milk Measurement Instrument |
| CN107525925A (en) * | 2017-10-12 | 2017-12-29 | 天津灵卫科技发展有限公司 | A kind of clenbuterol hydrochloride detection kit |
| CN107621464A (en) * | 2017-10-12 | 2018-01-23 | 天津灵卫科技发展有限公司 | A kind of compression type clenbuterol hydrochloride assay kit |
| CN109911425A (en) * | 2019-01-29 | 2019-06-21 | 新疆维吾尔自治区人民医院 | A kind of pulmonary tuberculosis detection kit and its application |
| CN117310157A (en) * | 2023-10-16 | 2023-12-29 | 青岛海润农大检测有限公司 | Device and method for detecting clenbuterol in animal food |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201489000U (en) * | 2009-06-01 | 2010-05-26 | 北京望尔康泰生物技术有限公司 | Colloidal gold test paper card for detecting clenbuterol drug residue |
| CN105044358A (en) * | 2015-07-13 | 2015-11-11 | 绍兴圣康生物科技有限公司 | Haptoglobin determination kit |
| CN206431145U (en) * | 2016-12-30 | 2017-08-22 | 天津灵卫科技发展有限公司 | A kind of clenbuterol hydrochloride assay kit |
-
2016
- 2016-12-30 CN CN201611251920.6A patent/CN106771167A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201489000U (en) * | 2009-06-01 | 2010-05-26 | 北京望尔康泰生物技术有限公司 | Colloidal gold test paper card for detecting clenbuterol drug residue |
| CN105044358A (en) * | 2015-07-13 | 2015-11-11 | 绍兴圣康生物科技有限公司 | Haptoglobin determination kit |
| CN206431145U (en) * | 2016-12-30 | 2017-08-22 | 天津灵卫科技发展有限公司 | A kind of clenbuterol hydrochloride assay kit |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389883A (en) * | 2017-08-10 | 2017-11-24 | 安徽优乐亿乳业有限公司 | A kind of multi-functional Milk Measurement Instrument |
| CN107525925A (en) * | 2017-10-12 | 2017-12-29 | 天津灵卫科技发展有限公司 | A kind of clenbuterol hydrochloride detection kit |
| CN107621464A (en) * | 2017-10-12 | 2018-01-23 | 天津灵卫科技发展有限公司 | A kind of compression type clenbuterol hydrochloride assay kit |
| CN109911425A (en) * | 2019-01-29 | 2019-06-21 | 新疆维吾尔自治区人民医院 | A kind of pulmonary tuberculosis detection kit and its application |
| CN117310157A (en) * | 2023-10-16 | 2023-12-29 | 青岛海润农大检测有限公司 | Device and method for detecting clenbuterol in animal food |
| CN117310157B (en) * | 2023-10-16 | 2024-02-13 | 青岛海润农大检测有限公司 | Device and method for detecting clenbuterol in animal food |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170531 |