CN206177961U - Chloramphenicol colloidal gold method immunity chromatography test paper card - Google Patents
Chloramphenicol colloidal gold method immunity chromatography test paper card Download PDFInfo
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- CN206177961U CN206177961U CN201621220033.8U CN201621220033U CN206177961U CN 206177961 U CN206177961 U CN 206177961U CN 201621220033 U CN201621220033 U CN 201621220033U CN 206177961 U CN206177961 U CN 206177961U
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- chloromycetin
- sample
- colloidal gold
- reagent chamber
- chromatography test
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Abstract
The utility model discloses a chloramphenicol colloidal gold method immunity chromatography test paper card belongs to the biotechnology field, including the test paper strip that is used for the detecting reagent chamber that the application of sample end of covering the surface kneaded dough and cover the lower surface of kneading dough, the application of sample end communicates through the circulation passageway with the reagent chamber, the reagent intracavity place gold -labeled antibody earlier with treat that sample article liquid contact back is direct and develop the color with epimembranal detection line and the matter accuse line contact of reaction, reduced on original test paper strip earlier will with the gold -labeled antibody contact after detect again, can make the sample and react liquid and react more abundant to reduce the error, increase the reaction sensitivity of whole system.
Description
Technical field
This utility model belongs to biological technical field, and in particular to a kind of chloromycetin colloidal gold method immune chromatography test card.
Background technology
Chloromycetin（Chloramphenicol, CAP）It is a kind of high-efficiency broad spectrum antibiotic, gram positive bacteria can be suppressed
And gram negative bacteria, the infectious disease control being widely used in all kinds of poultry, domestic animal, aquatic products production process.But chlorine
Residual of the mycin in food can constitute potential hazard to health, cause the bone marrow hematogenesis of people in disorder, cause regeneration
The developed countries such as the disease such as aplastic anemia, granular white blood cells deficiency disease and neonate, premature infant's Synthetic Grey disease, America and Europe phase
After forbidding or strictly prohibit the use of.The Ministry of Agriculture of China announces No. 235 file and also specifies, chloromycetin and its salt, ester（Including succinum
Chloromycetin）Must not detect in all Edible tissues of all food animals.
It is used at present detecting that the most popular method of chloromycetin is mainly gas chromatogram, liquid chromatograph and chromatography mass spectrometry, this
Although a little methods have the advantages that sensitivity is high, result is accurate, reproducible, false positive few, still suffers from certain shortcoming, such as sample
Product pretreatment process is complicated, and instrumentation degree is high and expensive, and analyze speed is slow, and developed country and food can not have gradually been met
Requirement of the product processing enterprise to detection method test limit.By comparison, immunological assay method sensitivity is higher, high specificity,
Sample pretreatment is simple, analysis time is short, is adapted to on-site supervision and the examination of great amount of samples.
The content of the invention
For the deficiencies in the prior art, the purpose of this utility model is directed to chloromycetin colloidal gold method immunity layer
Analysis test card.
For achieving the above object, this utility model is using such as purgation technical scheme：
A kind of chloromycetin colloidal gold method immune chromatography test card, including being fitted together to the plastic casing that connects together by base and cover
Body, is packaged with the test strips for detection in the plastic box body；Sample-adding end and observation window are provided with the cover；Its feature exists
In：The test strips are including base plate and are successively set on sample absorption pad, reaction film, adsorptive pads closely coupled on base plate,
The reaction film has the detection line and coating sheep anti mouse for being coated with the printing of chloromycetin hapten-carrier protein conjugate solution
The nature controlling line that IgG solution is printed, wherein detection line are located near the side of sample absorption pad；It is embedded with the cover lower surface
Reagent chamber, the cover lower surface that the reagent chamber outer is located is provided with draw-in groove and snaps connection with clamp, sets on the clamp
There is micropore, the reagent chamber is connected by circulation passage with sample-adding end, and the within the chamber surrounded by reagent chamber and clamp is provided with
Chloromycetin monoclonal antibody-colloid gold label thing；The reagent chamber is located at sample absorption pad top, and the observation window is located at reaction
The top of film is simultaneously it is observed that detection line and nature controlling line.
Further, protecting film is additionally provided with the base plate, the protecting film is located at the two ends of base plate, absorbs with sample respectively
The outward flange of pad is connected with the outward flange of adsorptive pads.So can preferably preserve reagent solution so that detection line and Quality Control
Line is fully contacted.
Further, the observation window is embedded in and is provided with lamella lucida, can prevent foreign body from contacting with test strips, and test strips are risen
To certain protective effect.
Further, detection line trace " C " and nature controlling line trace " T " are additionally provided with the cover, are easy to personnel directly to observe.
Further, the base plate is hard plastic bar or the cardboard bar not absorbed water；The sample absorption pad is glass fibre
Cotton；The adsorptive pads are the pure white filter paper by made by plant long fibre is raw material.
Because above-mentioned technical proposal is used, this utility model has compared with prior art following advantages：
This utility model provide chloromycetin colloidal gold method immune chromatography test card, with sensitivity height, high specificity, into
This low, simple to operate, detection time is short, be adapted to various units uses, storage is simple, the advantage of long shelf-life.Wherein, using height
The chloromycetin monoclonal antibody of specificity, it is ensured that the reliability of testing result；Gold labeling antibody is placed on into reagent intracavity, in inspection
During survey, gold labeling antibody can be made to be fully contacted with measuring samples liquid, fully reaction, so as to reduce error, increase whole body
The reaction sensitivity of system.
Description of the drawings
Fig. 1 is structural representation of the present utility model.
In figure：1. cover；2. base；3. end is loaded；4. circulation passage；5. reagent chamber；6. clamp；7. observation window；8. saturating
Isotropic disk；9. base plate；10. sample absorption pad；11. detection lines；12. reaction films；13. nature controlling lines；14. adsorptive pads；15. protecting film.
Specific embodiment
Below in conjunction with the accompanying drawings and embodiment is further described to this utility model：
Embodiment：
Shown in Fig. 1：Chloromycetin colloidal gold method immune chromatography test card, is fitted together to what is connected together including by base 2 and cover 1
Plastic box body, is packaged with the test strips for detection in the plastic box body.
Sample-adding end 3 and observation window 7 are provided with the cover 1, reagent chamber 5, the examination are embedded with the lower surface of the cover 1
The lower surface of cover 1 that the outer of agent chamber 5 is located is provided with draw-in groove and snaps connection with clamp 6, and the clamp 6 is provided with micropore, institute
State reagent chamber 5 to connect with sample-adding end 3 by circulation passage 4, it is mould that the within the chamber surrounded by reagent chamber 5 and clamp 6 is provided with chlorine
Plain monoclonal antibody-colloid gold label thing.Measuring samples liquid enters reagent chamber after instilling from sample-adding end along circulation passage, first with
Chloromycetin monoclonal antibody-colloid gold label thing carries out reaction and forms gold mark reactant liquor, and the gold marks reactant liquor from clamp
Micropore drippage is formed.A period of time, itself and chloromycetin monoclonal antibody-gold colloidal can be stayed due to examining sample liquid in reagent intracavity
Label has sufficient time of contact, and reaction is abundant.
The test strips include base plate 9 and are successively set on sample absorption pad 10, reaction film closely coupled on base plate 9
12nd, adsorptive pads 14, the reaction film 12 has the detection line for being coated with the printing of chloromycetin hapten-carrier protein conjugate solution
11 nature controlling lines 13 printed with coating sheep anti-mouse igg solution, wherein detection line 11 is located near the side of sample absorption pad 10.Institute
Reagent chamber 5 is stated positioned at the top of sample absorption pad 10, the observation window 7 be located at the top of reaction film 12 and it is observed that detection line and
Nature controlling line.
Furthermore, it is understood that being additionally provided with protecting film 15 on the base plate 9, the protecting film 15 is located at the two ends of base plate 9, respectively
It is connected with the outward flange of sample absorption pad 10 and the outward flange of adsorptive pads 14.So reagent solution can be preferably preserved, be made
Obtain detection line and nature controlling line is fully contacted.
Furthermore, it is understood that the observation window 7 is embedded in is provided with lamella lucida 8, can prevent foreign body from contacting with test strips, to examination
Paper slip plays certain protective effect.
Furthermore, it is understood that being additionally provided with detection line trace " C " and nature controlling line trace " T " on the cover 1, it is easy to personnel direct
Observation.
Base plate 9 used in the present embodiment is hard plastic bar or the cardboard bar not absorbed water, and the sample absorption pad 10 is
Glass fibre cotton, the adsorptive pads 14 are the pure white filter paper by made by plant long fibre is raw material.
When using, the measuring samples liquid after process is instilled from sample-adding end, measuring samples liquid is flowed into along circulation passage and tried
Agent intracavity and chloromycetin monoclonal antibody-colloid gold label thing（Gold labeling antibody）Together to reaction membrane diffusion with reference to after；If to be checked
The content of chloromycetin is high in sample liquid, then the chloromycetin in diffusion process in measuring samples liquid can enter in combination with gold labeling antibody
And in completely enclosed gold labeling antibody chloromycetin antigen-combining site, prevent gold labeling antibody with chloromycetin hapten-load on reaction film
Body protein conjugate is combined, and detection zone does not develop the color, and sheep anti-mouse igg（Nature controlling line）Then can be combined with gold labeling antibody, quality control region shows
Color；If the content of chloromycetin is low in measuring samples liquid or nothing, the antigen binding site in gold labeling antibody can not be closed, and then
Gold labeling antibody can be combined with chloromycetin hapten-carrier protein-coupled antigen on reaction film, detection zone colour developing, while sheep anti mouse
IgG（Nature controlling line）Also can be combined with gold labeling antibody, quality control region colour developing.If nature controlling line does not develop the color, test strips failure.
It is positive：Work as quality control region（C）Show red stripes, and detection zone（T）When not developing the color, the positive is judged to.
It is negative：Work as quality control region（C）Show red stripes, detection zone（T）Simultaneously red stripes are also showed that, be judged to the moon
Property.
It is invalid：Work as quality control region（C）Red stripes are not shown, then no matter detection zone（T）Whether show red stripes, should
Test strips are judged to invalid.
Below only most preferred embodiment of the present utility model is described, but is not to be construed as the limit to claim
System.This utility model is not limited only to above example, all to be made in the protection domain of this utility model independent claims
Various change is in protection domain of the present utility model.
Claims (5)
1. a kind of chloromycetin colloidal gold method immune chromatography test card, including being fitted together to the plastic casing that connects together by base and cover
Body, is packaged with the test strips for detection in the plastic box body；Sample-adding end and observation window are provided with the cover；Its feature exists
In：The test strips are including base plate and are successively set on sample absorption pad, reaction film, adsorptive pads closely coupled on base plate,
The reaction film has the detection line and coating sheep anti mouse for being coated with the printing of chloromycetin hapten-carrier protein conjugate solution
The nature controlling line that IgG solution is printed, wherein detection line are located near the side of sample absorption pad；It is embedded with the cover lower surface
Reagent chamber, the cover lower surface that the reagent chamber outer is located is provided with draw-in groove and snaps connection with clamp, sets on the clamp
There is micropore, the reagent chamber is connected by circulation passage with sample-adding end, and the within the chamber surrounded by reagent chamber and clamp is provided with
Chloromycetin monoclonal antibody-colloid gold label thing；The reagent chamber is located at sample absorption pad top, and the observation window is located at reaction
The top of film is simultaneously it is observed that detection line and nature controlling line.
2. chloromycetin colloidal gold method immune chromatography test card according to claim 1, it is characterised in that：On the base plate also
Protecting film is provided with, the protecting film is located at the two ends of base plate, respectively with the outward flange and the outward flange of adsorptive pads of sample absorption pad
It is connected.
3. chloromycetin colloidal gold method immune chromatography test card according to claim 1, it is characterised in that：In the observation window
It is embedded to be provided with lamella lucida.
4. chloromycetin colloidal gold method immune chromatography test card according to claim 1, it is characterised in that：On the cover also
It is provided with detection line trace " C " and nature controlling line trace " T ".
5. chloromycetin colloidal gold method immune chromatography test card according to claim 1, it is characterised in that：The base plate is hard
Matter plastic strip or the cardboard bar not absorbed water；The sample absorption pad is glass fibre cotton；The adsorptive pads are by plant long fibre
The pure white filter paper made by raw material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621220033.8U CN206177961U (en) | 2016-11-14 | 2016-11-14 | Chloramphenicol colloidal gold method immunity chromatography test paper card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621220033.8U CN206177961U (en) | 2016-11-14 | 2016-11-14 | Chloramphenicol colloidal gold method immunity chromatography test paper card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206177961U true CN206177961U (en) | 2017-05-17 |
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ID=58684321
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621220033.8U Active CN206177961U (en) | 2016-11-14 | 2016-11-14 | Chloramphenicol colloidal gold method immunity chromatography test paper card |
Country Status (1)
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| CN (1) | CN206177961U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109142719A (en) * | 2018-07-10 | 2019-01-04 | 广州佰芮慷生物科技有限公司 | A kind of detection method |
| CN109813896A (en) * | 2017-11-18 | 2019-05-28 | 镇江亿特生物科技发展有限公司 | Chloramphenicol medicament residue Rapid detection test strip |
-
2016
- 2016-11-14 CN CN201621220033.8U patent/CN206177961U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109813896A (en) * | 2017-11-18 | 2019-05-28 | 镇江亿特生物科技发展有限公司 | Chloramphenicol medicament residue Rapid detection test strip |
| CN109142719A (en) * | 2018-07-10 | 2019-01-04 | 广州佰芮慷生物科技有限公司 | A kind of detection method |
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