CN109142719A - A kind of detection method - Google Patents
A kind of detection method Download PDFInfo
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- CN109142719A CN109142719A CN201810774349.9A CN201810774349A CN109142719A CN 109142719 A CN109142719 A CN 109142719A CN 201810774349 A CN201810774349 A CN 201810774349A CN 109142719 A CN109142719 A CN 109142719A
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Abstract
The invention discloses a kind of detection devices, for quickly detecting the antibody in biological sample.This method avoid the generations of false negative result.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to a kind of antibody detection method.
Background technique
Quickly detection is in clinical detection field using more and more extensive.Wherein, immuno-chromatographic test paper strip is because its is simple and efficient
And it is used widely in clinical detection.In antibody test field, immuno-chromatographic test paper strip is mostly dual-antigen sandwich method.And
Connection is not then to receive, the reason is that, containing the antibody of high concentration in serum, when serum is added to immuno-chromatographic test paper strip
When sample pad, the antibody in serum can be in conjunction with the anti-human igg that the signal on bonding pad marks, but the antibody content in serum is remote
Greater than the anti-human igg on bonding pad, thus, detection signal will be greatly reduced, even without.One solution is using dilute
Serum is diluted by interpretation of the law, but can equally reduce positive rate in this way.So generally believing " indirect method immunity-chromatography test
Paper slip belongs to greatly rubbish ".So under normal circumstances, mostly using immunity percolation method to carry out indirect method and surveying antibody.But immunity percolation
Method needs multiple filling operation, affects the application of this method.Also have and use in different chromatography directions (such as IDEXX company
SNAP) and the modes of different swimming lanes (the binary channels immunochromatographydetection detection card of such as Chembio company) solves the problems, such as this, still
These immunochromatography product processes are complicated, with high costs, and are easy to appear because chromatography caused by overlap joint is not close loses
The problems such as losing.
Summary of the invention
Just " indirect method immuno-chromatographic test paper strip belongs to greatly rubbish " this problem provides a kind of detection method to the present invention.
The technical problems to be solved by the invention are achieved by the following technical programs:
A kind of immunochromatographic method detecting antibody, which is characterized in that have inspection in the chromatographic film of immuno-chromatographic test paper strip used
Survey line is coated with detection antigen at detection line；When detecting biological sample, biological sample firstly flows through chromatographic film, and signal label resists
Antibody subsequently passes through chromatographic film.
The immunochromatographic method of detection antibody as described above, which is characterized in that the antiantibody be refer to in sample
The biomolecule that specifically binds of antibody, can be secondary antibody, can also be Protein G, protein A, Protein
L, the biomolecule that can be specifically bound with antibody with the antigen of target antibody specific bond etc..
The immunochromatographic method of detection antibody as described above, which is characterized in that the load position of biological sample is located at sample
The load position at the nearly chromatographic film end of pad, the antiantibody of signal label is located at the remote chromatographic film end of sample pad.
The immunochromatographic method of detection antibody as described above, which is characterized in that the load position and signal post of biological sample
The load position of the antiantibody of note is same position: when detection biological sample, first plus biological sample, then plus signal label is anti-
Body.
The immunochromatographic method of detection antibody as described above, which is characterized in that the immuno-chromatographic test paper strip can also be
Including PVC bottom plate and successively overlap joint paste sample pad 1 on PVC bottom plate, sample pad 2, chromatographic film, blotting paper device；
When detecting biological sample, biological sample is added in the sample pad 2 of test strips first, then again adds the antiantibody that signal marks
Onto sample pad 1.
The immunochromatographic method of detection antibody as described above, which is characterized in that the signal be nanometer non-ferrous metal particle,
High polymer particle, fluorescent molecule, fluorescent grain etc. can be used as the substance of biomarker.
A kind of device detecting antibody, which is characterized in that including conjugate drop bottle and immuno-chromatographic test paper strip, conjugate drop
Antiantibody containing signal label in bottle.
The device of detection antibody as described above, which is characterized in that immuno-chromatographic test paper strip includes PVC bottom plate, Yi Jiyi
It is secondary to overlap sample pad, the chromatographic film, blotting paper being pasted on PVC bottom plate.
The device of detection antibody as described above, which is characterized in that have detection line and nature controlling line in chromatographic film, in detection line
It is coated with antigen.
The device of detection antibody as described above, which is characterized in that when detection biological sample, biological sample is first added dropwise to sample
On product pad, then it is added dropwise again on the antiantibody to sample pad of signal label.
The device of detection antibody as described above, which is characterized in that there is sample application zone 1 and sample application zone 2 two to add in sample pad
Sample area, sample application zone 1 are in far from chromatographic film end, and sample application zone 2 is in nearly chromatographic film end.
The device of detection antibody as described above, which is characterized in that when detection biological sample, biological sample is first added dropwise to sample
Then the antiantibody of signal label is added dropwise to the sample application zone of sample pad 1 in the sample application zone 2 of product pad again.
The device of detection antibody as described above, which is characterized in that the immuno-chromatographic test paper strip can also be immune layer
Analysis detection card, immunochromatographydetection detection card includes that immuno-chromatographic test paper strip and plastics get stuck.
The device of detection antibody as described above, which is characterized in that have a well on immunochromatographydetection detection card；Detection
When biological sample, first biological sample is added in well, then the antiantibody that signal marks is added in well again.
The device of detection antibody as described above, which is characterized in that have well 1 and well on immunochromatographydetection detection card
2 two wells, well 2 are in the position of nearly chromatographic film；When detecting biological sample, biological sample is first added to well 2
In, then the antiantibody that signal marks is added in well 1 again.
A kind of device detecting antibody, which is characterized in that including detection micropore and immuno-chromatographic test paper strip, detect in micropore
Antiantibody containing signal label；The immuno-chromatographic test paper strip includes PVC bottom plate and successively overlap joint pastes PVC bottom plate
On sample pad, chromatographic film, blotting paper；When detecting biological sample, first biological sample is added in sample pad and is chromatographed, then again will
In the detection micropore for the antiantibody that sample pad one end insertion of test strips is marked containing signal.
The invention has the following beneficial effects:
The present invention with regard to indirect method is not suitable for immunochromatography and detects this problem proposing a kind of easy-to-use solution party
Case.This method has abandoned the cumbersome card production course of immunity percolation card, has been not easy to the problem of batch production, has combined immune layer
The advantages such as analysis test strips are easy to use, are easy to produce in batches, it is ensured that the sensitivity of detection.
Detailed description of the invention
Fig. 1 is immuno-chromatographic test paper strip structure chart of the present invention
5: detection line；6: nature controlling line；7: sample pad；8: chromatographic film；9: blotting paper；10:PVC bottom plate.
Fig. 2 is immuno-chromatographic test paper strip of the present invention (two sample pads) structure chart
5: detection line；6: nature controlling line；8: chromatographic film；9: blotting paper；10:PVC bottom plate；11: sample pad 1；12: sample pad 2.
Fig. 3 is immunochromatographydetection detection card structure chart of the present invention
17: well；3: peep hole；4: plastics get stuck；5: detection line；6: nature controlling line.
Fig. 4 is immunochromatographydetection detection card of the present invention (double wells) structure chart
1: well 1；2: well 2；3: peep hole；4: plastics get stuck.
Specific embodiment
The present invention will be described in detail with reference to the accompanying drawings and embodiments, and embodiment is only preferred implementation of the invention
Mode is not limitation of the invention.
The present invention is described further with reference to embodiments.
Embodiment 1 detects the Brucella antibody in serum
(1) preparation of chromatographic film: brucella lipopolysaccharides (LPS) is diluted to 2mg/mL, according to 0.8 μ L/cm in nitric acid
It crosses on cellulose membrane (chromatographic film), as detection line.Rabbit-anti sheep IgG is diluted to 0.2mg/mL, according to 0.8 μ L/cm in nitre
It crosses on acid cellulose film (chromatographic film), as nature controlling line.
(2) chromatographic film for pulling line: being pasted the middle position of PVC bottom plate by the assembling of test strips, keeps detection line close
Sample pad direction, nature controlling line is close to blotting paper direction.Then, sample pad and blotting paper are pasted in corresponding position, makes sample
Pad has the overlap joint of about 1mm or so with chromatographic film, blotting paper and chromatographic film.Finally it is cut into the immuno-chromatographic test paper strip of 3.5mm.Preparation
Good immuno-chromatographic test paper strip includes PVC bottom plate and successively overlap joint pastes sample pad, the chromatographic film, water suction on PVC bottom plate
Paper.It is placed in dry environment, it is spare.
(3) preparation of the antiantibody of signal label: 10mL nanogold (30nm) solution is taken, 100 μ L 0.2M potassium carbonate are added
Then 200 μ g goat anti-human iggs are added in solution, mix, be stored at room temperature 20min；Then 1mL 10%BSA is added and closes 20min.
4 DEG C of centrifugation 10min of 10000rpm, discard supernatant.Liquid (10mM Tris-HCl, pH7.5) is redissolved with 10mL, and nanogold is resuspended
Grain, is placed in 4 DEG C, spare.
(4) it detects the Brucella antibody in human serum: test strips is lain against on table top, 5 μ L serum of addition are in immune
In the sample pad of chromatograph test strip, 2 drop (about 100 μ L) nanogold particles are then added dropwise and mark goat anti-human igg, are observed after 10min
As a result.
Can also detect as follows: 5 μ L serum of addition are in the sample pad of immuno-chromatographic test paper strip, by test strips
Sample pad end insertion containing 200 μ L nanogold particles label goat anti-human igg detection micropore in, result is observed after 15min.
10 times will be diluted with a cloth bacterium patients serum with the negative serum containing various concentration antibody, be implemented respectively with this
Scheme provided by example and the serum after the detection dilution of conventional indirect process test strip, testing result are as shown in table 1.It can see
Out, the present embodiment anti-interference is significantly better than conventional indirect process test strip.
Influence of the different antibodies concentration for testing result in 1 serum of table
| Antibody concentration in serum (g/L) | 5 | 10 | 15 | 20 | 25 | 30 |
| The present embodiment | +++ | +++ | +++ | +++ | +++ | ++ |
| Conventional indirect process test strip | +++ | +++ | ++ | + | - | - |
Note: traditional test strip used is the immuno-chromatographic test paper strip prepared using identical raw material.Including PVC bottom plate, with
And successively overlap joint pastes sample pad, bonding pad, chromatographic film, blotting paper on PVC bottom plate.There is signal to mark on bonding pad anti-
Antibody.Coated at test strips detection line is antigen (LPS).The goat anti-human igg of coated nano gold mark on bonding pad.
+ positive is represented ,+more positives are stronger；Represent feminine gender.
Embodiment 2 detects the mycobacterium tuberculosis antibody in milk
(1) preparation of chromatographic film: being diluted to 1mg/mL for tubercle bacillus MPT83 albumen, according to 0.8 μ L/cm in nitric acid fibre
It ties up and crosses on plain film (chromatographic film), as detection line.Rabbit-anti ox IgG is diluted to 0.2mg/mL, according to 0.8 μ L/cm in nitric acid
It crosses on cellulose membrane (chromatographic film), as nature controlling line.
(2) chromatographic film for pulling line: being pasted the middle position of PVC bottom plate by the assembling of test strips, keeps detection line close
Sample pad direction, nature controlling line is close to blotting paper direction.Then, sample pad and blotting paper are pasted in corresponding position, makes sample
Pad has the overlap joint of about 1mm or so with chromatographic film, blotting paper and chromatographic film.Finally it is cut into the immuno-chromatographic test paper strip of 3.5mm.Preparation
Good immuno-chromatographic test paper strip includes PVC bottom plate and successively overlap joint pastes sample pad 1, the sample pad 2, layer on PVC bottom plate
Analyse film, blotting paper.It is placed in dry environment, it is spare.
(3) preparation of the antiantibody of signal label: 10mL nanogold (30nm) solution is taken, 100 μ L 0.2M potassium carbonate are added
Then 200 μ g Protein G are added in solution, mix, be stored at room temperature 20min；Then 1mL 10%BSA is added and closes 20min.
4 DEG C of centrifugation 10min of 10000rpm, discard supernatant.Liquid (10mM Tris-HCl, pH7.5) is redissolved with 10mL, and nanogold is resuspended
Grain, is placed in 4 DEG C, spare.
(4) it detects the mycobacterium tuberculosis antibody in milk: test strips is lain against on table top, add 25 μ L milk in immune layer
In the sample pad 2 for analysing test strips, result is observed after 80 μ L nanogold particles label protein G, 10min is then added dropwise.
(5) table 2 is the testing result that the present embodiment detects mycobacterium tuberculosis antibody in 141 parts of milk samples, with ELISA method
It is completely the same.
Table 2 detects milk sample with the present embodiment method and ELISA respectively
Embodiment 3 detects the aleutian disease virus antibody in mink serum
(1) preparation of chromatographic film: being diluted to 1mg/mL for aleutian disease virus VP2 albumen, according to 0.8 μ L/cm in nitric acid fibre
It ties up and crosses on plain film (chromatographic film), as detection line.Goat-anti mink IgG is diluted to 0.2mg/mL, according to 0.8 μ L/cm in nitre
It crosses on acid cellulose film (chromatographic film), as nature controlling line.
(2) chromatographic film for pulling line: being pasted the middle position of PVC bottom plate by the assembling of test strips, keeps detection line close
Sample pad direction, nature controlling line is close to blotting paper direction.Then, sample pad and blotting paper are pasted in corresponding position, makes sample
Pad has the overlap joint of about 1mm or so with chromatographic film, blotting paper and chromatographic film.Finally it is cut into the immuno-chromatographic test paper strip of 3.5mm.Preparation
Good immuno-chromatographic test paper strip includes PVC bottom plate and successively overlap joint pastes sample pad, the chromatographic film, water suction on PVC bottom plate
Paper.
The assembling of immunochromatographydetection detection card: the immuno-chromatographic test paper strip cut is fitted into during plastics get stuck, on plastics get stuck
There are peep hole and well.Peep hole is for observing testing result.Well is used to expose the sample pad of immuno-chromatographic test paper strip
Position, for adding the antiantibody of Xue Qing blood plasma and signal label.
(3) preparation of the antiantibody of signal label: colloid carbon particle is purchased from Beijing Jingtai Meikang Biological Science & Technology Co., Ltd..
Goat-anti mink IgG is marked referring to kit specification.
(4) it detects the aleutian disease virus antibody in mink serum: immunochromatographydetection detection card is lain against on table top, add 5 μ
L mink serum observes result after goat-anti the mink IgG, 10min of 80 μ L colloid carbon markings is then added dropwise in well.
(5) table 3 is the testing result that the present embodiment detects aleutian disease virus antibody in 135 parts of mink serum samples, with
ELISA method is completely the same.
Table 3 detects mink serum sample with the present embodiment method and ELISA respectively
Embodiment 4 detects the antibodies against foot-and-mouth disease virus in serum
(1) preparation of chromatographic film: aftosa 3ABC albumen is diluted to 1mg/mL, according to 0.8 μ L/cm in nitrocellulose
It crosses on film (chromatographic film), as detection line.Goat-anti pig IgG is diluted to 0.2mg/mL, according to 0.8 μ L/cm in cellulose nitrate
It crosses on plain film (chromatographic film), as nature controlling line.
(2) chromatographic film for pulling line: being pasted the middle position of PVC bottom plate by the assembling of test strips, keeps detection line close
Sample pad direction, nature controlling line is close to blotting paper direction.Then, sample pad and blotting paper are pasted in corresponding position, makes sample
Pad has the overlap joint of about 1mm or so with chromatographic film, blotting paper and chromatographic film.Finally it is cut into the immuno-chromatographic test paper strip of 3.5mm.Preparation
Good immuno-chromatographic test paper strip includes PVC bottom plate and successively overlap joint pastes sample pad, the chromatographic film, water suction on PVC bottom plate
Paper.
The assembling of immunochromatographydetection detection card: the immuno-chromatographic test paper strip cut is fitted into during plastics get stuck, on plastics get stuck
There are peep hole, well 1 and well 2.Peep hole is for observing testing result.Well 2 is for exposing immune chromatography test paper
The sample pad of item, for add serum blood plasma；The sample pad of the exposure immuno-chromatographic test paper strip of well 1, for adding signal post
The antiantibody of note.
(3) preparation of the antiantibody of signal label: 10mL nanogold (40nm) solution is taken, 100 μ L 0.2M potassium carbonate are added
Then 200 μ g ProteinA are added in solution, mix, be stored at room temperature 20min；Then 1mL 10%BSA is added and closes 20min.
4 DEG C of centrifugation 10min of 10000rpm, discard supernatant.Liquid (10mM Tris-HCl, pH7.5) is redissolved with 10mL, and nanogold is resuspended
Grain, is placed in 4 DEG C, spare.
(4) it detects the antibodies against foot-and-mouth disease virus in pig, sheep and cow's serum: immunochromatographydetection detection card is lain against on table top,
5 μ L serum are added in well 2, the proteinA of 80 μ L nanogold particles label are then added dropwise into well 1,10min
After observe result.
(5) table 4 be the present embodiment detect 157 portions of pigs, ox, in sheep blood serum sample antibodies against foot-and-mouth disease virus testing result,
It is completely the same with ELISA method.
Table 4 detects aftosa sample with the present embodiment method and ELISA respectively
Embodiment 5 redissolves influence of the glucan for testing result in liquid
It is our surprising discovery that addition glucan can effectively improve signal strength in redissolving liquid, detection background is reduced,
Improve the sensitivity of detection.It is described further by taking embodiment 1 as an example below.
The glucan of various concentration is added in reciprocal solution, the antiantibody of gravity treatment nanogold particle label detects 10 parts of yin
Positive sample (5 parts of positives, 5 parts of feminine genders), each case replication of each sample 3 times.Testing result is as shown in table 5 below.
Table 5 redissolves influence of the glucan for testing result in liquid
Note: 2/3 represents detection 3 times, and 2 times testing result is the positive, other are same
Embodiment 6 detects HCV (Hepatitis C Virus) antibody in serum
(1) preparation of chromatographic film: HCV envelope antigen (purchased from luxuriant and rich with fragrance roc biology) is diluted to 2mg/mL, according to 0.8 μ L/cm
It crosses in nitrocellulose filter (chromatographic film), as detection line.Bovine serum albumin(BSA) (BSA) polyclonal antibody is diluted to
0.2mg/mL crosses according to 0.8 μ L/cm in nitrocellulose filter (chromatographic film), as nature controlling line.
(2) chromatographic film for pulling line: being pasted the middle position of PVC bottom plate by the assembling of test strips, keeps detection line close
Sample pad direction, nature controlling line is close to blotting paper direction.Then, sample pad and blotting paper are pasted in corresponding position, makes sample
Pad has the overlap joint of about 1mm or so with chromatographic film, blotting paper and chromatographic film.Finally it is cut into the immuno-chromatographic test paper strip of 3.5mm.Preparation
Good immuno-chromatographic test paper strip includes PVC bottom plate and successively overlap joint pastes sample pad, the chromatographic film, water suction on PVC bottom plate
Paper.It is placed in dry environment, it is spare.
(3) preparation of the antiantibody of signal label: 10mL nanogold (30nm) solution is taken, 100 μ L 0.2M potassium carbonate are added
Then solution is added 200 μ g HCV detection antigen (purchased from luxuriant and rich with fragrance roc biology), mixes, be stored at room temperature 20min；Then 1mL is added
10%BSA closes 20min.4 DEG C of centrifugation 10min of 10000rpm, discard supernatant.With 10mL redissolve liquid (10mM Tris-HCl,
PH7.5 nanogold particle) is resuspended, is placed in 4 DEG C, it is spare.
(4) it detects the HCV antibody in human serum: test strips is lain against on table top, add 5 μ L serum in immunity-chromatography test
In the sample pad of paper slip, 2 drop (about 100 μ L) nanogold particle label detection antigens are then added dropwise, observe result after 10min.
Can also detect as follows: 5 μ L serum of addition are in the sample pad of immuno-chromatographic test paper strip, by test strips
The detection micropore of HCV detection antigen that mark containing 200 μ L nanogold particles of sample pad end insertion in, observation is tied after 15min
Fruit.
10 times will be diluted with portion HCV Positive Sera with the negative serum containing various concentration antibody, respectively with this
Scheme provided by embodiment and the serum after the detection dilution of conventional indirect process test strip, testing result are as shown in table 1.It can
To find out, the present embodiment anti-interference is significantly better than conventional indirect process test strip.
Influence of the different antibodies concentration for testing result in 1 serum of table
| Antibody concentration in serum (g/L) | 5 | 10 | 15 | 20 | 25 | 30 |
| The present embodiment | +++ | +++ | +++ | +++ | +++ | ++ |
| Conventional indirect process test strip | +++ | +++ | ++ | + | - | - |
Note: traditional test strip used is the immuno-chromatographic test paper strip prepared using identical raw material.Including PVC bottom plate, with
And successively overlap joint pastes sample pad, bonding pad, chromatographic film, blotting paper on PVC bottom plate.The sheep for thering is signal to mark on bonding pad
Anti-human igg.Coated at test strips detection line is antigen (HCV envelope antigen).The sheep of coated nano gold mark on bonding pad
Anti-human igg.
+ positive is represented ,+more positives are stronger；Represent feminine gender.
Embodiments of the present invention above described embodiment only expresses, the description thereof is more specific and detailed, but can not
Therefore limitations on the scope of the patent of the present invention are interpreted as, as long as skill obtained in the form of equivalent substitutions or equivalent transformations
Art scheme should all be fallen within the scope and spirit of the invention.
Claims (16)
1. a kind of immunochromatographic method for detecting antibody, which is characterized in that have detection in the chromatographic film of immuno-chromatographic test paper strip used
Line is coated with detection antigen at detection line；When detecting biological sample, biological sample firstly flows through chromatographic film, and resisting for signal label is anti-
Body subsequently passes through chromatographic film.
2. the immunochromatographic method of detection antibody as described in claim 1, which is characterized in that the antiantibody is to refer to and sample
The biomolecule that antibody in this is specifically bound, can be secondary antibody, can also be Protein G, protein A,
The biomolecule that Protein L and the antigen of target antibody specific bond etc. can be specifically bound with antibody.
3. the immunochromatographic method of detection antibody as described in claim 1, which is characterized in that the load position of biological sample is located at
The load position at the nearly chromatographic film end of sample pad, the antiantibody of signal label is located at the remote chromatographic film end of sample pad.
4. the immunochromatographic method of detection antibody as described in claim 1, which is characterized in that the load position and letter of biological sample
The load position of the antiantibody of labelled notation is same position: when detection biological sample, first plus biological sample, then plus signal label
Antiantibody.
5. the immunochromatographic method of detection antibody as described in claim 1, which is characterized in that the immuno-chromatographic test paper strip may be used also
To be to include PVC bottom plate and successively overlap joint pastes sample pad 1, sample pad 2, chromatographic film, blotting paper on PVC bottom plate
Device；When detecting biological sample, biological sample is added in the sample pad 2 of test strips first, then again by the anti-of signal label
Antibody is added in sample pad 1.
6. the immunochromatographic method of detection antibody as described in claim 1, which is characterized in that the signal is nanometer non-ferrous metal
Particle, high polymer particle, fluorescent molecule, fluorescent grain etc. can be used as the substance of biomarker.
7. a kind of device for detecting antibody, which is characterized in that including conjugate drop bottle and immuno-chromatographic test paper strip, conjugate drop bottle
In containing signal label antiantibody.
8. the device of detection antibody as claimed in claim 7, which is characterized in that immuno-chromatographic test paper strip includes PVC bottom plate, with
And successively overlap sample pad, the chromatographic film, blotting paper being pasted on PVC bottom plate.
9. the device of detection antibody as claimed in claim 7, which is characterized in that have detection line and nature controlling line in chromatographic film, examine
Antigen is coated on survey line.
10. the device of detection antibody as claimed in claim 7, which is characterized in that when detection biological sample, biological sample is first added dropwise
In sheet to sample pad, then it is added dropwise again on the antiantibody to sample pad of signal label.
11. the device of detection antibody as claimed in claim 7, which is characterized in that have sample application zone 1 and sample application zone 2 in sample pad
Two sample application zones, sample application zone 1 are in far from chromatographic film end, and sample application zone 2 is in nearly chromatographic film end.
12. the device of detection antibody as claimed in claim 7, which is characterized in that when detection biological sample, biological sample is first added dropwise
This arrives the sample application zone 2 of sample pad, and the antiantibody of signal label is then added dropwise again to the sample application zone of sample pad 1.
13. the device of detection antibody as claimed in claim 7, which is characterized in that the immuno-chromatographic test paper strip can also be
Immunochromatographydetection detection card, immunochromatographydetection detection card include that immuno-chromatographic test paper strip and plastics get stuck.
14. the device of detection antibody as claimed in claim 13, which is characterized in that there is a sample-adding on immunochromatographydetection detection card
Hole；When detecting biological sample, first biological sample is added in well, the antiantibody that signal marks then is added to well again
In.
15. the device of detection antibody as claimed in claim 13, which is characterized in that there is well 1 on immunochromatographydetection detection card
With 2 two wells of well, well 2 is in the position of nearly chromatographic film；When detecting biological sample, first biological sample is added to
In well 2, then the antiantibody that signal marks is added in well 1 again.
16. a kind of device for detecting antibody, which is characterized in that including detection micropore and immuno-chromatographic test paper strip, detect in micropore
Antiantibody containing signal label；The immuno-chromatographic test paper strip includes PVC bottom plate and successively overlap joint pastes PVC bottom plate
On sample pad, chromatographic film, blotting paper；When detecting biological sample, first biological sample is added in sample pad and is chromatographed, then again will
In the detection micropore for the antiantibody that sample pad one end insertion of test strips is marked containing signal.
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