CN102323407A - Phthalate quick test card and test method thereof - Google Patents
Phthalate quick test card and test method thereof Download PDFInfo
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- CN102323407A CN102323407A CN201110155182A CN201110155182A CN102323407A CN 102323407 A CN102323407 A CN 102323407A CN 201110155182 A CN201110155182 A CN 201110155182A CN 201110155182 A CN201110155182 A CN 201110155182A CN 102323407 A CN102323407 A CN 102323407A
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Abstract
The invention relates to a phthalate quick test card and a test method thereof, belonging to the technical field of phthalate testing. According to the phthalate quick test card provided by the invention, a test strip is arranged in a housing of the phthalate quick test card and comprises a sample pad, a colloidal gold membrane or an emulsion particle membrane, a cellulose nitrate membrane and a water-absorbing membrane which are sequentially adhered on a supporting backplate; the colloidal gold membrane is a glass-fiber membrane containing an anti-phthalate monoclonal antibody colloidal gold mark, or the emulsion particle membrane is a glass-fiber membrane containing an containing an anti-phthalate monoclonal antibody emulsion particle mark; and two test bands are arranged on the cellulose nitrate membrane, one is the test band containing phthalate protein conjugates, and the other is a quality control band containing anti-rabbit antibodies or anti-rat antibodies. The phthalate quick test card and the test method thereof provided by the invention have the advantages that plastic toys, the content of phthalate in plastic packages, beverages and other samples can be directly tested by applying an immunological method, and the phthalate quick test card has the advantages of easiness in preparation, convenience and quickness in use and accurate results.
Description
Technical field
The present invention relates to a kind of phthalic ester rapid detection card and detection method thereof, is a kind of appliance that detects phthalic ester, belongs to phthalic ester detection technique field.
Background technology
Phthalic ester (claiming phthalate ester again, abbreviation PAE) is the general designation of the ester of phthalic acid formation.Comprise dioctyl phthalate, phthalic acid two (2-ethylhexyl) ester (DEHP), dibutyl phthalate (DBP), diethyl phthalate (DEP), diamyl phthalate (DPP), diisononyl phthalate (DINP) etc.When being used as plastic plasticizer, generally refer to the ester of the alcohol formation of phthalic acid and 4～15 carbon.
Phthalic ester is one type of chemicals that can play emollescence.It is widely used in hundreds of products such as toy, packaging for foodstuff, vinyl flooring, wallpaper, detersive, nail polish, spray, shampoo and bath foam.Owing to do not form covalent bond between phthalic ester and the plastic matrix, but be connected with hydrogen bond with Van der Waals force, maintenance chemical property independently separately each other, thereby in touching packaged food when contained water, grease, just can stripping.A few days ago, appear at adding phthalic acid two (2-ethylhexyl) ester (DEHP) incident in the food additives again in Taiwan.Research shows that phthalic ester can disturb endocrine, makes the minimizing of mankind spermatozoon quantity, low, the paramophia of locomitivity, and serious also can cause necrozoospermia and carcinoma of testis, is " arch-criminal " who causes the male genetic problem.
Phthalic ester is one type of global poisonous persistent organic pollutants, and American National Environmental Protection Agency and China Environmental Monitoring General Station all are listed as it and are priority pollutants.The contained DEHP of the plastics of all toys of the 2005/84/EC of European Union command request or child care products, DBP, and BBP concentration surpass 0.1% must not sell in European Union market.At present, the main method of detection plastic products or Phthalic Acid Esters in Food has high performance liquid chromatography and LC-MS etc. to carry out qualitative and quantitative analysis.These methods require high to experimental instrument and equipment, complex operation is high to operating personnel's professional knowledge requirement, wastes time and energy.Therefore setting up a kind of analytical approach that detects Phthalic Acid Esters in Food accurately and reliably again quickly and easily is very important.
The present invention sends out bright a kind of based on having antigenic immunological detection method after the small-molecule substance phthalic ester coupling protein matter; Take immunology competition ratio juris; A kind of quick detection reagent of association colloid gold or latex particle labelling technique and immunochromatographic method design can detect the phthalic ester in the samples such as plastic toy, plastics package, beverage fast specifically.The phthalic acid ester content in the samples such as plastic toy, plastics package, beverage is judged in the colour developing that has or not according to test card sample detection line and nature controlling line.Reaction is to utilize phthalic ester protein conjugate antigenicity and a kind of detection method of setting up, has the advantage of high specificity, the flase drop problem that can avoid the false positive of other detection method to cause.Test card is simple to operate simultaneously need not special instrument and equipment fast, is fit to execute-in-place.Because sample size needs less, easy fast, be applicable to the examination of great amount of samples.The result is accurate, and is highly sensitive, and accuracy rate is big.
Summary of the invention
The object of the present invention is to provide a kind of appliance that can detect phthalic ester; Directly the method for applied immunology detects the content of phthalic ester in the samples such as plastic toy, plastics package, beverage; Overcome the shortcomings such as complex operation that existing chromatography detects, set up based on the specific detection method of immunology.
Technical scheme of the present invention: a kind of phthalic ester rapid detection card, it is for being provided with test-strips 2 in the test card shell 1 of rectangular flat shell shape, and the test card case surface has fenestra of detection 3 and well 4; Test-strips 2 is formed by pasting sample pad 8, collaurum film or latex particle film 7, nitrocellulose filter 6 and 9 of absorbing membranes above that successively through adhesive sticker on the supporting backboard 5; The stacked stickup nitrocellulose filter 6 in supporting backboard middle part; One endlap of supporting backboard is put and is pasted absorbing membrane 9; The stacked stickup sample pad 8 of the other end; Absorbing membrane is inner to overlap with nitrocellulose filter respectively with sample pad the inner separately, at the clinch of sample pad and nitrocellulose filter, inserts and puts between the two and pastes one section collaurum film or latex particle film 7; The collaurum film is the glass fibre membrane that contains anti-phthalic ester monoclonal antibody colloid gold label; Or the latex particle film is the glass fibre membrane that contains anti-phthalic ester monoclonal antibody latex particle mark; There is one to detect band and a quality control band on the nitrocellulose filter; Be successively: the detection that contains the phthalic ester protein conjugate is with 10, contains the quality control band 11 of anti-rabbit antibody or anti-mouse antibody, and test-strips is inserted in the test card shell; Sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
Adopt above-mentioned phthalic ester rapid detection card, test sample is plastic toy, plastics package, beverage, fruit juice etc., and disposal route is following:
A, plastic products: plastic toy, plastics package sample, plastic sample is pulverized, accurately take by weighing 2g and be soaked in the sample diluting liquid of 5mL, 300W microwave treatment 0.5-2min is dissolved in the dilution phthalic ester in the sample, gets solution then and detects.
B, liquid beverage: sports drink, samples of juice, directly press 1:1 and dilute back to be checked with sample diluting liquid.
The PBST solution of sample diluting liquid: pH7.4,0.01mol/L includes 0.15mol/L NaCl, mass concentration 0.5% Tween-20.
When detection be with 10 with quality control band 11 manifest brownish red trace band simultaneously, testing result is negative, representes that phthalic acid ester content does not exceed standard in the sample to be detected.
Be with 10 Show Colors not when detection, have only quality control band 11 to manifest the brownish red trace, testing result is positive, representes that phthalic acid ester content exceeds standard in the sample to be detected.
Be with 10 all not develop the color with quality control band 11 when detection, show that then test-strips lost efficacy.
Beneficial effect of the present invention: advantage of the present invention is to detect the phthalic ester that contains in the samples such as plastic toy, plastics package, beverage, is easy to preparation and saves testing cost, and is easy to use, detects fast, and highly sensitive, the result is accurate.
Description of drawings
Fig. 1 phthalic ester rapid detection card shell and test-strips arrangement figure in the enclosure.1, shell, 2, test-strips, 3, detect fenestra, 4, well.
Show the trace synoptic diagram on the nitrocellulose filter of Fig. 2 test-strips.6, nitrocellulose filter, 10, detect band, 11, quality control band.
The detection strip structural representation of Fig. 3 phthalic ester rapid detection card.5, supporting backboard, 6, nitrocellulose filter, 7, collaurum film or latex particle film, 8, sample pad, 9, absorbing membrane.
Embodiment
The present invention combines specific embodiment to further specify as follows referring to accompanying drawing:
Embodiment 1
A kind of phthalic ester rapid detection card, it is that test-strips 2 is set in test card shell 1, the setting of the structure of test card shell 1 and test-strips 2 is referring to accompanying drawing 1; Test card shell 1 is rectangular flat shell shape shell; Long 70mm, wide 20mm, thick 5mm; Shell wall thickness 1mm is processed by engineering plastics.Test card shell 1 is connected with the shell block two halves by cap and constitutes, and fenestra of detection 3 and well 4 are arranged on test card shell 1 cap.Test-strips 2 is placed in the shell block of test card shell 1.
Test-strips 2 is fillet thin slices of sandwich construction, and sheet is about 60mm, the wide about 4mm of sheet, the about 2.5mm of thickness.Test-strips 2 is sandwich constructions: bottom is a supporting backboard 5, is the igelite thin slice, thick about 0.5mm, long 60mm, wide 4mm; The stacked stickup nitrocellulose filter 6 in supporting backboard 5 middle parts, cellulose nitrate thickness 0.5mm, long 20-25mm, wide 4mm.Two isolated horizontal demonstration trace bands are arranged on the nitrocellulose filter 6, show in the trace band referring to 2, two in accompanying drawing, one is to detect to be with 10, contains the phthalic ester protein conjugate; Another is a quality control band 11, contains anti-rabbit antibody or anti-mouse antibody.Stacked stickup absorbing membrane 9 on supporting backboard 5 one ends; Stacked stickup sample pad 8 on supporting backboard 5 the other end; Absorbing membrane 9 the inners overlap with nitrocellulose filter 6 one ends respectively with sample pad 8 the inners separately; At the clinch of sample pad 8 with nitrocellulose filter 6; Insert and put between the two and paste one section collaurum film or latex particle film 7, the collaurum film is the glass fibre membrane that contains anti-phthalic ester monoclonal antibody colloid gold label, or the latex particle film is the glass fibre membrane that contains anti-phthalic ester monoclonal antibody latex particle mark.Collaurum film or latex particle film 7 thick 0.5-0.8mm, long 5mm, wide 4mm.Sample pad 8 thick 0.5-0.8mm, long 20mm, wide 4mm.Absorbing membrane 9 thick 0.5-0.8mm, long 16-20mm, wide 4mm.Collaurum film or latex particle film 7, sample pad 8 and absorbing membrane 9 are 1-2mm with the nitrocellulose filter lap of splice.Absorbing membrane 9 materials are thieving paper, and sample pad 8 materials are water adsorption glass tunica fibrosas.
Phthalic ester test card of the present invention is used for detecting the detection of the phthalic ester of samples such as plastic toy, plastics package, beverage.
Embodiment 2
The disposal route of sample is following:
A, plastic products: plastic toy, plastics package sample, plastic sample is pulverized, accurately take by weighing 2g and be soaked in the sample diluting liquid of 5mL, 300W microwave treatment 0.5-2min is dissolved in the dilution phthalic ester in the sample, gets solution then and detects.
B, liquid beverage: sports drink, samples of juice, directly press 1:1 and dilute back to be checked with sample diluting liquid.
The PBST solution of sample diluting liquid: pH7.4,0.01mol/L includes 0.15mol/L NaCl, 0.5% Tween-20.
When using the phthalic ester rapid detection card to detect; Earlier sample liquid to be detected is instilled into from well 4 on the sample pad 8 of phthalic ester rapid detection card; Because syphonic effect principle; Drive contained anti-phthalic ester monoclonal antibody collaurum of sample liquid to be detected and collaurum film or latex particle film 7 or latex particle labels together to nitrocellulose filter 6 diffusions, observations in 5-10 minute.
The key reaction of test card is immunologic antigen and antibody response, on nitrocellulose filter, moves
The antibody of collaurum or latex particle mark, on calibration tape respectively with contain the phthalic ester protein conjugate, and the anti-rabbit antibody of quality control band or anti-mouse antibody reaction forms the brownish red band.If have phthalic ester to be checked to exist in the sample, after sample adds, promptly react, and can not react with contained phthalic ester protein conjugate, thereby do not develop the color with detection with antibody.The result of key reaction has following 3 kinds of situation:
1, when detection be with 10 and quality control band 11 manifest brownish red trace band simultaneously, the expression testing result negative, explain that phthalic acid ester content does not exceed standard in the sample to be detected.
2, be with 10 Show Colors not when detection, have only quality control band 11 to manifest the brownish red trace, testing result is positive, explains that phthalic acid ester content exceeds standard in the sample to be detected.
3, when detection be with 10 and quality control band 11 all do not develop the color, show that then detecting strip lost efficacy.
Claims (2)
1. phthalic ester rapid detection card, it is characterized in that: in the test card shell (1) of rectangular flat shell shape, test-strips (2) is set, the test card case surface has detection fenestra (3) and well (4); Test-strips (2) is upward pasted sample pad (8), collaurum film or latex particle film (7), nitrocellulose filter (6) and absorbing membrane (9) above that successively through adhesive sticker by supporting backboard (5) and is formed; Supporting backboard middle part stacked stickup nitrocellulose filter (6); One endlap of supporting backboard is put and is pasted absorbing membrane (9); The stacked stickup sample pad of the other end (8); Absorbing membrane is inner to overlap with nitrocellulose filter respectively with sample pad the inner separately, at the clinch of sample pad and nitrocellulose filter, inserts and puts between the two and pastes one section collaurum film or latex particle film (7); The collaurum film is the glass fibre membrane that contains anti-phthalic ester monoclonal antibody colloid gold label; Or the latex particle film is the glass fibre membrane that contains anti-phthalic ester monoclonal antibody latex particle mark; There is one to detect band and a quality control band on the nitrocellulose filter; Be successively: contain the detection band (10) of phthalic ester protein conjugate, contain the quality control band (11) of anti-rabbit antibody or anti-mouse antibody, test-strips is inserted in the test card shell; Sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
2. with the detection method of the described phthalic ester rapid detection card of claim 1; The content that it is characterized in that phthalic ester in the direct test sample of method of applied immunology; Test sample is plastic toy, plastics package or beverage, fruit juice, and disposal route is following:
A, plastic toy, plastics package sample: plastic sample is pulverized, accurately taken by weighing 2g and be soaked in the sample diluting liquid of 5mL, 300W microwave treatment 0.5-2min is dissolved in the dilution phthalic ester in the sample, gets solution then and detects;
B, beverage, samples of juice: directly press 1:1 and dilute back to be checked with sample diluting liquid;
The PBST solution of sample diluting liquid: pH7.4,0.01mol/L includes 0.15mol/L NaCl, 0.5% Tween-20;
When detection be with 10 with quality control band 11 manifest brownish red trace band simultaneously, testing result is negative, representes that phthalic acid ester content does not exceed standard in the sample to be detected;
Be with 10 Show Colors not when detection, have only quality control band 11 to manifest the brownish red trace, testing result is positive, representes that phthalic acid ester content exceeds standard in the sample to be detected;
Be with 10 all not develop the color with quality control band 11 when detection, show that then test-strips lost efficacy.
Priority Applications (1)
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| CN201110155182A CN102323407A (en) | 2011-06-10 | 2011-06-10 | Phthalate quick test card and test method thereof |
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| CN201110155182A CN102323407A (en) | 2011-06-10 | 2011-06-10 | Phthalate quick test card and test method thereof |
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| CN102323407A true CN102323407A (en) | 2012-01-18 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103760337A (en) * | 2013-12-11 | 2014-04-30 | 安徽师范大学 | Indirect competive enzyme-linked immunosorbent assay detection method of bis(2-ethylhexyl)phthalate |
| CN106680493A (en) * | 2017-01-21 | 2017-05-17 | 上海为然环保科技有限公司 | Agricultural product pesticide residual colloidal gold detection kit |
| CN106841602A (en) * | 2017-01-21 | 2017-06-13 | 上海源紊新能源科技有限公司 | A kind of diallyl phthalate colloidal gold immunochromatographydetection detection test paper |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103760337A (en) * | 2013-12-11 | 2014-04-30 | 安徽师范大学 | Indirect competive enzyme-linked immunosorbent assay detection method of bis(2-ethylhexyl)phthalate |
| CN103760337B (en) * | 2013-12-11 | 2015-10-21 | 安徽师范大学 | The Indirect cELISA detection method of phthalic acid (2-ethyl hexyl) ester |
| CN106680493A (en) * | 2017-01-21 | 2017-05-17 | 上海为然环保科技有限公司 | Agricultural product pesticide residual colloidal gold detection kit |
| CN106841602A (en) * | 2017-01-21 | 2017-06-13 | 上海源紊新能源科技有限公司 | A kind of diallyl phthalate colloidal gold immunochromatographydetection detection test paper |
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Application publication date: 20120118 |