CN106680493A - Agricultural product pesticide residual colloidal gold detection kit - Google Patents
Agricultural product pesticide residual colloidal gold detection kit Download PDFInfo
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- CN106680493A CN106680493A CN201710044709.5A CN201710044709A CN106680493A CN 106680493 A CN106680493 A CN 106680493A CN 201710044709 A CN201710044709 A CN 201710044709A CN 106680493 A CN106680493 A CN 106680493A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The invention discloses an agricultural product pesticide residual colloidal gold detection kit. The detection kit comprises a box body (11), a cover body (12) and a box base (13), wherein the box body (12) is positioned at the top of the box body (11), and the box base (13) is arranged at the bottom of the box body (11); and a plurality of placement holes are formed in the box base (13), and test paper (18), sample bottles (14) and detection plates (17) for carrying the test paper (18) are respectively fixedly arranged in the placement holes. The detection kit is simple to operate and short in time consumption, does not need impurity removing pretreatment for a detected sample, is favorable to carrying out work for grass-roots units with general condition, and is convenient for field quick detection of related departments.
Description
Technical field
The present invention relates to technical field of biological, more particularly to a kind of agricultural product drug residue gold-immunochromatographyreagent reagent for assay
Box.
Background technology
Pesticide residues (pesticide residues) refer to be not completely broken down in latter period of Pesticide use and
Remain in trace pesticide substance, toxic metabolite, the degradation product in organism, cutting, pesticide residues soil, water body, air
With the general name of impurity.Pesticide Residue with pesticide a large amount of productions and widely use and produce, up to the present, in the world
Chemical pesticide annual production is nearly 2,000,000 tons, there are about more than 1000 kinds of artificial-synthetic compound and is used as insecticide, antibacterial, defoliant
Etc. a large amount of administrations of class pesticide, especially organic agricultural chemicals, serious Pesticides Residues are caused, the pesticide of residual enters grain
In the food such as food, veterinary antibiotics, fish, shrimp, meat, egg, food pollution is caused, serious threat is constituted to health.
At present, most countries and area are usually all kinds of using plastic sheeting, plastic fresh-keeping membrane or plastic material packaging
Agricultural product, in these plastics plasticiser is contained, wherein, because didecyl phthalate is phthalate
(PAEs) material can allow hard plastic glue to become resilient plastic cement, be widely used, and research shows, PAEs class materials are in human body
Breeding unsoundness can be caused, the major diseases such as fetal anomaly are even resulted in, it is used together not only to food with pesticide residue
Thing causes dual pollution, and the threat to health is more doubled and redoubled.
Because at present test pesticide residue and PAEs classes material need to adopt the complex operation such as liquid phase or gas chromatography mass spectrometry chromatograph
And the high large-scale instrument and equipment of support price is detected, on the one hand the start of these instruments and maintenance cost are expensive, are unfavorable for
Economic and practical, on the other hand, the detection time of these equipment is longer, it is impossible to provide result in time.Therefore, a kind of cost is needed badly
The fast device of low, simple to operate, detection speed.Based on this, the present invention provides a kind of agricultural product drug residue gold colloidal detection examination
Agent box.
The content of the invention
For the deficiency that above-mentioned prior art is present, the invention provides a kind of detection examination of agricultural product drug residue gold colloidal
Agent box, to solve above-mentioned technical problem.
The present invention is achieved through the following technical solutions：
A kind of agricultural product drug residue gold-immunochromatographyreagent reagent for assay box, including box body, lid and box base, lid is located at box body
Top, box base is arranged at the bottom of box body；Multiple putting holes are provided with the box base, putting hole hole is installed with respectively
Test paper, sample bottle and detection plate.
Preferably, the inwall of the sample bottle is provided with graduation mark.
Preferably, the inwall of the detection plate is provided with detection graduation mark.
Preferably, the Test paper is didecyl phthalate colloidal gold immunochromatographydetection detection test paper.
Preferably, the didecyl phthalate colloidal gold immunochromatographydetection detection test paper includes sample layer, gold colloidal mark
Note layer, detection conversion zone, water accepting layer and backing；Didecyl phthalate monoclonal anti is coated with the colloid gold label layer
The anti-organophosphors specific antibody of body-colloid gold label thing and enzyme labelling, in the detection conversion zone detection line T and matter are provided with
Control line C, is coated with the envelope antigen of didecyl phthalate-carrier conjugates in detection line T；The nature controlling line C is coated with
Rabbit anti-mouse igg and enzyme；The detection gold labeling antibody with detection envelope antigen association reaction and can develop the color, and detect
The site combined with antigen-specific with only one of which in gold labeling antibody；The sample layer, colloid gold label layer and detection conversion zone
Combine successively on backer board the same face according to order from left to right, from top to bottom, the colloid gold label layer and detection are anti-
The end for answering layer overlaps；The water accepting layer combines the other end in detection conversion zone.
Relative to prior art, beneficial effects of the present invention：The pesticide residues gold-immunochromatographyreagent reagent for assay box is placed, transporter
Just, simple to operate, the holding time is long, it is adaptable to the examination of Site Detection and batch samples.The present invention has high specificity；Spirit
Sensitivity is high, reaches 9.63ng/ml, and detection speed is fast, flux is big, simple to operation, colour stable, detection temperature optimum range
Wide, reagent paper long shelf-life and it is with low cost the advantages of, and half-quantitative detection can be realized, be suitable for food and medicine, environmental protection, inspection
The departments such as quarantine are used for quickly detecting to the didecyl phthalate in sample, and the present invention need not coordinate large-scale instrument to make
With the poor grass-roots unit of many experiment conditions can also easily carry out detection, therefore with wide market prospect and promote meaning
Justice.
Description of the drawings
Fig. 1 is the structural representation of the present invention.
Fig. 2 is the schematic enlarged-scale view of the Test paper shown in Fig. 1.
Wherein：1- sample layers, 2- colloid gold label layers, 3- detection conversion zones, 4- water accepting layers, 7- backer boards, 11- box bodys,
12- lids, 13- box bases, 14- sample bottles, 17- detection plates, 18- Test papers, the detection lines of T1- first, T2- second is detected
Line, the nature controlling lines of C1- first, the nature controlling lines of C2- second, the nature controlling lines of C3- the 3rd.
Specific embodiment
With the following Examples the invention will be further described.
Fig. 1 is the structural representation of the present invention, as shown in figure 1, a kind of agricultural product drug residue gold-immunochromatographyreagent reagent for assay box,
Including box body 11, lid 12 and box base 13, lid 11 is located at the top of box body 11, and box base 13 is arranged at the bottom of box body 11
Portion；Multiple putting holes are provided with the box base 13, putting hole hole is installed with respectively Test paper 18, sample as shown in Figure 2
Product bottle 14 and detection plate 17.
Preferably, the inwall of the sample bottle 14 is provided with graduation mark.
Preferably, the inwall of the detection plate 17 is provided with detection graduation mark.
Preferably, the Test paper 18 is didecyl phthalate colloidal gold immunochromatographydetection detection test paper.Such as Fig. 2 institutes
Show, the didecyl phthalate colloidal gold immunochromatographydetection detection test paper includes being prepared from by the material with water absorbing properties
Sample layer 1, colloid gold label layer 2, detection conversion zone 3, water accepting layer 4 and the backer board 7 that is prepared from by plastics.
Didecyl phthalate monoclonal antibody-colloid gold label thing and enzyme are coated with the colloid gold label layer 2
The anti-organophosphors specific antibody of labelling, in the present embodiment, the species of enzyme can adopt horseradish peroxidase, alkaline phosphatase
Enzyme or beta galactosidase, organophosphors are in parathion-methyl, fenthion, compd 22/190, Azodrin or thiometon
One or more mixing；Detection line T and nature controlling line C are provided with the detection conversion zone 3, detection line T is 1~3, matter
Control line C is 1~3, and the envelope antigen of didecyl phthalate-carrier conjugates is coated with detection line T；The nature controlling line
C is coated with rabbit anti-mouse igg and enzyme.In the present embodiment, detection line T includes the first inspection arranged successively as shown in Figure 2
Survey line T1 and the second detection line T2；Nature controlling line C includes the first nature controlling line C1, the second nature controlling line C2 and the 3rd Quality Control arranged successively
Line C3；Strict control colloid gold labeling antibody, didecyl phthalate-carrier conjugates and rabbit anti-mouse igg in the present embodiment
Ratio；Can according to different detection line or nature controlling line whether develop the color and color the depth, judge in sample liquid whether containing neighbour
Phthalic acid didecyl and organophosphors, it is possible to determine that the material concentration difference to be checked in sample, realize half-quantitative detection.
The detection gold labeling antibody with detection envelope antigen association reaction and can develop the color, and detection gold labeling antibody
The site that upper only one of which is combined with antigen-specific；The sample layer 1, colloid gold label layer 2 and detection conversion zone 3 are according to from a left side
Combine successively on the same face of backer board 7 to right, order from top to bottom, the colloid gold label layer 2 and detection conversion zone 3
End overlaps；The water accepting layer 4 combines the other end in detection conversion zone 3.
In the present embodiment, the gold labeling antibody is didecyl phthalate monoclonal antibody with phthalic acid didecyl
Ester-carrier protein couplet thing after antigen-immunized animal used as preparing.Didecyl phthalate-the carrier conjugates
Carrier mass used is protein, protein fragments, synthesis polypeptide, semi-synthetic polypeptide, polysaccharide, or the carrier mass is
Other synthesis of active group or natural polymer, such as diphtheria toxin, diphtherotoxin, tetanus toxin, yeast, nylon, dextrorotation Portugal gather
Sugar, cellulose or hemicellulose；In order to reach more preferable effect, the carrier mass further can select bovine serum albumin,
Oralbumin, keyhole limpet hemocyanin, Elityran or L- poly-D-lysines etc..
The operation principle of the Test paper employed in the present invention is as follows：
Effects of the nature controlling line C played in Test paper is no matter whether to contain didecyl phthalate in sample solution
Or organophosphors, move on in the coated gold labeling antibody of colloid gold label layer 2 up to can be with the rabbit on nature controlling line C during nature controlling line C
Anti- Mus IgG or enzyme set, aggregation forms macroscopic red granules, and nature controlling line C develops the color all the time, if tested
Cheng Zhong, nature controlling line C does not show, then it represents that test invalidation.
If having didecyl phthalate or organophosphors in testing sample, solution to be measured is absorbed and is led in absorbed layer
Capillarity forward movement is crossed, the small-molecule substance didecyl phthalate or organophosphors in sample can be moved quickly,
When colloid gold label layer 2 is moved to, will be with the coated didecyl phthalate monoclonal antibody of the gold colloidal on this layer
Or the anti-organophosphors specific antibody of enzyme labelling is combined, because anti-the having of didecyl phthalate monoclonal antibody enzyme labelling
Machine phosphorus specific antibody only one of which antigen binding site, and in sample free state didecyl phthalate binding ability ratio
The didecyl phthalate of parataxic it is strong, so when combine free state didecyl phthalate gold colloidal mark resist
When body continues to move to detection line T, it is impossible to combined with the didecyl phthalate-carrier conjugates being coated in detection line T,
Now detection line is colourless, when the colloid gold labeling antibody for combining the didecyl phthalate of free state is continued to move along,
When nature controlling line C is moved to, can be combined with the rabbit anti-mouse igg or organophosphors that are coated on nature controlling line, now nature controlling line is red
Color.I.e. when detection line T is colourless, when nature controlling line is red, testing result is the positive.
If without didecyl phthalate or organophosphors in testing sample, solution to be measured is absorbed and is passed through in absorbed layer
Capillarity forward movement, the coated didecyl phthalate colloid gold labeling antibody of colloid gold label layer 2 or enzyme labelling
Anti- organophosphors specific antibody is also moved forward, when reaching detection line T just with didecyl phthalate-carrier or organophosphors-
Carrier conjugates are combined, and now detection line T is redness, the anti-organophosphors specific antibody of colloid gold labeling antibody or enzyme labelling after
It is continuous to move forward, when nature controlling line C is moved to, can be combined with the rabbit anti-mouse igg or enzyme that are coated on nature controlling line, now
Nature controlling line is redness.I.e. when detection line T is redness, when nature controlling line C is red, testing result is feminine gender.
Strict control colloid gold labeling antibody of the invention, the anti-organophosphors specific antibody of enzyme labelling, phthalic acid didecyl
The ratio of ester-carrier conjugates and rabbit anti-mouse igg and enzyme.During concrete application, can be according to different detection line or parameter
Line whether develop the color and color the depth, judge whether containing didecyl phthalate or organophosphors in sample liquid, and
Content it is many with it is few, realize half-quantitative detection.
When two detection line T1s and T2 show red, then sample for it is negative (i.e. without didecyl phthalate or
Organophosphors)；When detection line T1 colour developing, when detection line T2 does not develop the color, then contain didecyl phthalate or organic in sample
Phosphorus, but its concentration is not high；When two detection lines do not develop the color, then in sample didecyl phthalate or organophosphors it is dense
Degree is very high, and with this half-quantitative detection is carried out.
When detection line T color is deeper than reference line C 3, then sample (does not contain didecyl phthalate O-phthalic for negative
Sour didecyl or organophosphors or denier)；When detection line T color is deeper than C3 is shallower than reference line C 2, then sample contains adjacent benzene
Dioctyl phthalate didecyl or organophosphors, concentration ratio is relatively low；When detection line T color is deeper than C2 is shallower than C1, then containing neighbour in sample
Phthalic acid didecyl or organophosphors, concentration is higher；When detection line T does not develop the color, then contain phthalic acid in sample
Didecyl or organophosphors, concentration is very high, and with this half-quantitative detection is carried out.
The present invention is due to the anti-organophosphors specific antibody immunity using didecyl phthalate gold colloidal and enzyme labelling
Test paper, the site that only one of which is combined with antigen-specific in the anti-organophosphors specific antibody of gold labeling antibody enzyme labelling, tool
There is high specificity；Sensitivity is high, reaches 9.63ng/ml, detection speed is fast, flux is big, simple to operation, colour stable, inspection
Testing temperature optimum range width, reagent paper long shelf-life and it is with low cost the advantages of, and half-quantitative detection can be realized.The quick detection
Test strips all can be used at 4~40 DEG C, just observable result after 5~15min, be suitable for food and medicine, environmental protection, inspection and quarantine
The didecyl phthalate or organophosphorus pesticide in sample is used for quickly detecting Deng department.
Didecyl phthalate detection is carried out to same beverage using different detection methods, its sensitivity (inspection is contrasted
Go out the limit) and accuracy.
1 three kinds of methods of table are detected to the didecyl phthalate in beverage
| Detection limit (ng/ml) | Accuracy | |
| Test paper | 9.63 | 98.7% |
| HPLC | 12.47 | 98.2% |
| GC | 11.39 | 98.6% |
Organophosphorus pesticide detection is carried out to same Fructus Cucumidis sativi using different detection methods, its sensitivity (detection is contrasted
The limit) and accuracy, as shown in table 2.
Table 2
| Detection limit (ng/ml) | Accuracy | |
| Test paper | 9.20 | 99.6% |
| HPLC | 13.45 | 97.2% |
| GC | 9.99 | 99.1% |
Can be known by Tables 1 and 2, using the didecyl phthalate in the detection paper beverage of the present invention, with tradition
Method HPLC compare with GC methods, detection limit is below HPLC and GC methods, i.e. sensitivity and is more up to 9.63ng/ml, accuracy
Compare almost with HPLC with GC methods.Detection limit is below HPLC and GC methods during detection Fructus Cucumidis sativi surface organophosphorus residue.Therefore
Using the present invention provide test kit have it is simple to operate, take it is short, the pretreatment of remove impurity need not be carried out to detection sample, have
Beneficial to condition, general grass-roots unit carries out the work, and being also convenient for relevant departments carries out field quick detection.
Finally it should be noted that above example is only illustrating technical scheme, rather than to present invention guarantor
The restriction of shield scope, although having made to explain to the present invention with reference to preferred embodiment, one of ordinary skill in the art should
Work as understanding, technical scheme can be modified or equivalent, without deviating from the reality of technical solution of the present invention
Matter and scope.
Claims (6)
1. a kind of agricultural product drug residue gold-immunochromatographyreagent reagent for assay box, it is characterised in that including box body (11), lid (12) and box
Base (13), lid (12) is arranged at the bottom of box body (11) positioned at the top of box body (11), box base (13)；The box base
(13) multiple putting holes are provided with, putting hole hole is installed with respectively Test paper (18), sample bottle (14) and for carrying inspection
The detection plate (17) of test paper (18).
2. a kind of agricultural product drug residue gold-immunochromatographyreagent reagent for assay box according to claim 1, it is characterised in that the sample
The inwall of product bottle (14) is provided with graduation mark.
3. a kind of agricultural product drug residue gold-immunochromatographyreagent reagent for assay box according to claim 2, it is characterised in that the inspection
The inwall for surveying disk (17) is provided with detection graduation mark.
4. a kind of agricultural product drug residue gold-immunochromatographyreagent reagent for assay box according to claim 3, it is characterised in that the inspection
Test paper (18) is didecyl phthalate colloidal gold immunochromatographydetection detection test paper.
5. a kind of agricultural product drug residue gold-immunochromatographyreagent reagent for assay box according to claim 4, it is characterised in that the neighbour
Phthalic acid didecyl colloidal gold immunochromatographydetection detection test paper includes sample layer (1), colloid gold label layer (2), detection conversion zone
(3), water accepting layer (4) and backer board (7)；Didecyl phthalate monoclonal anti is coated with the colloid gold label layer (2)
The anti-organophosphors specific antibody of body-colloid gold label thing and enzyme labelling, on the detection conversion zone (3) detection line T is provided with
With nature controlling line C, the envelope antigen of didecyl phthalate-carrier conjugates is coated with detection line T；The nature controlling line C bags
There are rabbit anti-mouse igg and enzyme；The detection gold labeling antibody with detection envelope antigen association reaction and can develop the color, and
The site that only one of which is combined with antigen-specific in detection gold labeling antibody；The sample layer (1), colloid gold label layer (2) and
Detection conversion zone (3) is combined successively on backer board (7) the same face according to order from left to right, from top to bottom, the colloid
The end of golden mark layer (2) and detection conversion zone (3) overlaps；The water accepting layer (4) is combined in the another of detection conversion zone (3)
End.
6. a kind of agricultural product drug residue gold-immunochromatographyreagent reagent for assay box according to claim 5, it is characterised in that the neighbour
The detection line of phthalic acid didecyl colloidal gold immunochromatographydetection detection test paper is 1~3, and nature controlling line is 1~3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710044709.5A CN106680493A (en) | 2017-01-21 | 2017-01-21 | Agricultural product pesticide residual colloidal gold detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710044709.5A CN106680493A (en) | 2017-01-21 | 2017-01-21 | Agricultural product pesticide residual colloidal gold detection kit |
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| Publication Number | Publication Date |
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| CN106680493A true CN106680493A (en) | 2017-05-17 |
Family
ID=58859861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710044709.5A Pending CN106680493A (en) | 2017-01-21 | 2017-01-21 | Agricultural product pesticide residual colloidal gold detection kit |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200986548Y (en) * | 2005-04-15 | 2007-12-05 | 华南农业大学 | Streptomycin quick semi-quantitative determination reagent paper |
| CN102323407A (en) * | 2011-06-10 | 2012-01-18 | 无锡安迪生物工程有限公司 | Phthalate quick test card and test method thereof |
| CN102539753A (en) * | 2011-12-29 | 2012-07-04 | 深圳康美生物科技股份有限公司 | Reagent kit and enzyme-linked immunochromatography for detecting various organophosphorus pesticide residues |
| CN103204925A (en) * | 2013-03-28 | 2013-07-17 | 江南大学 | Synthetic method of general artificial antigen of phthalate plasticizers for immunodetection |
| CN204495835U (en) * | 2015-02-15 | 2015-07-22 | 大理学院 | A kind of viral hepatitis type E detection kit |
| CN105044357A (en) * | 2015-07-13 | 2015-11-11 | 绍兴圣康生物科技有限公司 | Fibronectin determination kit |
| CN205120720U (en) * | 2015-06-05 | 2016-03-30 | 军科正源（北京）药物研究有限责任公司 | Kit of anti medicine antibody of short -term test EGFR antibody medicine |
| CN105606800A (en) * | 2016-01-27 | 2016-05-25 | 广州聚佰生物科技有限公司 | Broad-spectrum pesticide detection gold-colloidal strip and method |
| CN105785021A (en) * | 2016-04-08 | 2016-07-20 | 广州万联生物科技有限公司 | Quick detecting card for immunochromatography of organophosphorus and carbamate pesticide multiresidue cholinesterase |
| CN205484361U (en) * | 2016-01-16 | 2016-08-17 | 新疆医科大学 | Detect kit of hepatitis E virus igM antibody |
-
2017
- 2017-01-21 CN CN201710044709.5A patent/CN106680493A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200986548Y (en) * | 2005-04-15 | 2007-12-05 | 华南农业大学 | Streptomycin quick semi-quantitative determination reagent paper |
| CN102323407A (en) * | 2011-06-10 | 2012-01-18 | 无锡安迪生物工程有限公司 | Phthalate quick test card and test method thereof |
| CN102539753A (en) * | 2011-12-29 | 2012-07-04 | 深圳康美生物科技股份有限公司 | Reagent kit and enzyme-linked immunochromatography for detecting various organophosphorus pesticide residues |
| CN103204925A (en) * | 2013-03-28 | 2013-07-17 | 江南大学 | Synthetic method of general artificial antigen of phthalate plasticizers for immunodetection |
| CN204495835U (en) * | 2015-02-15 | 2015-07-22 | 大理学院 | A kind of viral hepatitis type E detection kit |
| CN205120720U (en) * | 2015-06-05 | 2016-03-30 | 军科正源（北京）药物研究有限责任公司 | Kit of anti medicine antibody of short -term test EGFR antibody medicine |
| CN105044357A (en) * | 2015-07-13 | 2015-11-11 | 绍兴圣康生物科技有限公司 | Fibronectin determination kit |
| CN205484361U (en) * | 2016-01-16 | 2016-08-17 | 新疆医科大学 | Detect kit of hepatitis E virus igM antibody |
| CN105606800A (en) * | 2016-01-27 | 2016-05-25 | 广州聚佰生物科技有限公司 | Broad-spectrum pesticide detection gold-colloidal strip and method |
| CN105785021A (en) * | 2016-04-08 | 2016-07-20 | 广州万联生物科技有限公司 | Quick detecting card for immunochromatography of organophosphorus and carbamate pesticide multiresidue cholinesterase |
Non-Patent Citations (1)
| Title |
|---|
| 王梅等: "蔬菜中邻苯二甲酸酯的残留研究", 《中国农学通报》 * |
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