CN205120720U - Kit of anti medicine antibody of short -term test EGFR antibody medicine - Google Patents
Kit of anti medicine antibody of short -term test EGFR antibody medicine Download PDFInfo
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- CN205120720U CN205120720U CN201520382279.4U CN201520382279U CN205120720U CN 205120720 U CN205120720 U CN 205120720U CN 201520382279 U CN201520382279 U CN 201520382279U CN 205120720 U CN205120720 U CN 205120720U
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Abstract
The utility model relates to an immunoassay medical science field specifically is a kit of anti medicine antibody of short -term test EGFR antibody medicine. This kit includes box body and lid, characterized by: the box body bottom is equipped with holds in the palm with the measure -alike double layer of plastic of box body, plastics ask the upper surface to be equipped with the bottle and hold in the palm for place acidolysis solution reagent bottle in, and the measuring bottle of solution reagent bottle and EDTA -K2 anticoagulant, on holding in the palm, plastics still are equipped with the detection card slot of placing EGFR antibody medicine peridium, in holding in the palm, the lid still is equipped with the intermediate layer of placing the low temperature ice bag with plastics. The utility model discloses the kit has saved sample transportation and loaded down with trivial details reagent and has prepared and reaction sequence through adopting simple and convenient detection card and ice bag cooling, and convenience that the greatly increased kit used can use in bed side or patient family, and the shortcoming of having avoided the sample probably to change in the transportation has improved the detection efficiency and the accuracy of anti medicine antibody.
Description
Technical field
The utility model relates to field of immunoassay medicine, is specially a kind of kit of anti-medicine antibody of quick detection EGFR antibody drug.
Background technology
EGFR antibody class medicine, as Erbitux, Victibix etc., all belong to biotech drug.As heterologous material, EGFR antibody class medicine, particularly mouse or mosaic EGFR antibody drug, have certain immunogenicity, when being administered in human body, immunogenicity can impel the immune response of body thus the antibody (anti-drugantibody, ADA) that develops immunity to drugs.ADA mono-aspect can make body spontaneous removing EGFR antibody drug, then causes drug therapy to be lost efficacy; Immune system is removed by ADA in the process of EGFR antibody drug and may be caused other biological respinse on the other hand, thus causes subsidiary reaction to occur.Therefore, the ADA level of accurately and rapidly monitoring patient after accepting medicine is requisite link in biotech drug Clinical practice process, and for the immunogenicity assessing this type of medicine, to set up suitable ADA detection method very necessary.ADA detects and usually adopts MBP enzyme linked immuno-adsorbent assay technology (ELISA) to analyze.Traditional ADA detects in ELISA, is be used for catching by microwell plate by medicine bag, then adds sample and kind antibody detects, and the method shortcoming to detect the ADA of single type, as the ADA of IgG type or IgM type.Bridge-type enzyme linked immunosorbent assay (Bridging-ELISA) is then comparatively advanced ADA detection method, its principle is the feature utilizing antibody molecule to contain more than 2 antigen binding sites, Exogenous therapies medicine (X) is coated in ELISA Plate, add the positive polyclonal antibody (ADAX-P) for medicine and biotin labeled exogenous drugs (Biotin-X) subsequently simultaneously, abundant reaction and washing after add streptavidin mark superoxide horseradish enzyme (SA-HRP), then add superoxide horseradish zymolyte and carry out chromogenic reaction, finally read absorbance value by sulfuric acid cessation reaction.This method specialty in drug research process is efficient, but for during medication patient, then efficiency is very low clinically: first need sample to send clinical laboratory, reviewer detects through mark and obtains result, consuming time longer, and may degrade in sample transport process, testing result is affected.
Utility model content
The technical matters that the utility model solves is the kit providing a kind of anti-medicine antibody for detecting EGFR antibody drug fast, to solve the problem proposed in above-mentioned background technology.
The technical matters that the utility model solves realizes by the following technical solutions:
A kind of kit of the anti-medicine antibody for detecting EGFR antibody drug fast, comprise box body and lid, the plastic tray measure-alike with box body is provided with bottom box body, described plastic tray upper surface is provided with bottle holder, for placing sample preparation bottle, acidolysis solution reagent bottle, neutralization solution reagent bottle, described plastic tray is also provided with the test card slot placing EGFR antibody drug bag quilt, described lid and plastic tray are double layer design, there is the interlayer placing low temperature ice bag inside, to keep the stability of solution in kit.
Beneficial effect
The utility model kit is by adopting easy test card and ice bag cooling, eliminate sample transport and loaded down with trivial details preparation of reagents and course of reaction, greatly increase the convenience that kit uses, can use in bed side or patient family, avoid the shortcoming that sample may change in transportation, improve detection efficiency and the accuracy of anti-medicine antibody.
Accompanying drawing explanation
Fig. 1 is the utility model reagent cartridge configuration schematic diagram; Wherein
1: box body; 2: lid; 3: plastic tray; 4: ice bag interlayer; 5: sample preparation bottle; 6: acidolysis solution bottle; 7: neutralization solution bottle; 8: test card draw-in groove; 9: gold mark test card.
Fig. 2 is gold mark test card structural representation; Wherein
10: sample pad; 11: gold mark pad; 12: nitrocellulose filter; 13: detection line T; 14: nature controlling line C; 15: adsorptive pads.
Fig. 3 is that testing result judges schematic diagram; Wherein
9: gold mark test card; 16: loading district; 17: the area of observation coverage.
Embodiment
In order to make actualizing technology means of the present utility model, creation characteristic, reach object and effect is easy to understand, below in conjunction with concrete diagram, set forth the utility model further.
As shown in Figure 1, a kind of kit of the anti-medicine antibody for detecting EGFR antibody drug fast, comprise box body 1 and lid 2, the plastic tray 3 measure-alike with box body 1 is provided with bottom box body 1, described plastic tray 3 is placed sample preparation bottle 5, acidolysis solution reagent bottle 6, neutralization solution reagent bottle 7, described plastic tray 3 is also provided with slot 8, and for placing the test card 9 of EGFR antibody drug bag quilt, described plastic tray 3 and lid 2 are equipped with the interlayer 4 placing low temperature ice bag.
As shown in Figure 2 described, test card is made up of PVC board, and the two ends of test card are respectively equipped with sample pad 10 and absorption pad 15, and middle part is provided with nitrocellulose filter detection layers 12.Nitrocellulose filter detection layers 12 and sample pad 10 intersection, be provided with the gold mark pad 11 of the EGFR antibody drug (Au-X) that bag is marked by gold, gold mark pad 11 one end portion is arranged under sample pad 1, and the other end is divided and is arranged on nitrocellulose filter detection layers 12.In the detection layers 12 continued with sample pad 10, be provided with detection line T13 and nature controlling line C14, detection line 13 be coated with EGFR antibody drug (X), nature controlling line 14 is coated with the positive antibody (ADA-P) of anti-egfr antibodies medicine.Gold mark pad 11 is arranged under sample pad 10, is provided with the overlay region of 5mm with sample pad; Gold mark pad 11 is arranged on nitrocellulose filter detection layers 12, is provided with the overlay region of 2mm with nitrocellulose filter detection layers.
The utility model kit is used to detect the embodiment of the anti-medicine antibody of EGFR
1. sample collection: finger tip is taken a blood sample, drips in containing EDTA-K
2in the sample preparation bottle of anti-coagulants.
2. detection method:
To in sample preparation bottle, drip 5 acetolysis liquid, after reacting half an hour, then be added dropwise to 5 Tris neutralizers, shake up.Then test card is kept flat, get solution in bottle, drip 4 ~ 5 and (drip in the middle part of sample application zone, to ensure that test result is accurate) in the sample application zone 16 of test card as far as possible, leave standstill wait 5 minutes.Under the effect of absorbent material, measuring samples slowly moves to detection zone and quality control region, and film reaction system starts.No matter there is anti-medicine antibody in sample to be checked, quality control region C place all should manifest a coloured panel, and this is important criterion, can determine whether the sample adding q.s, whether chromatography process is normal, simultaneously also can as judging the quality control standard whether test card is qualified.
3. result judges:
If when in measuring samples, anti-medicine antibody concentration is higher than detection threshold, the anti-medicine antibody of EGFR then in sample be fixed on the medicine in the detection zone on film and be combined, thus there will be a red detection zone at the T line place of the area of observation coverage 17, represent positive (+) result, as shown in Fig. 3 .a.(note: because the concentration of medicine antibody anti-in measuring samples is different, the phenomenon of shade may appear in the coloured panel thus in test section.No matter but the coloured panel shade within a certain period of time in test section is all judged to be the positive; If anti-medicine antibody concentration is lower than Monitoring lower-cut in measuring samples, then there will not be detection zone in detection zone, represent negative (-) result, namely only in quality control region, occur a color belt, as shown in Fig. 3 .b.
If there is not coloured panel in quality control region, then testing process failure.Possible reason is that operating process is incorrect, kit has gone bad damage or occur quality problems, as shown in Fig. 3 .c or 3.d.
The utility model is by adopting gold mark test card and ice bag Cryo Equipment, increase the range of application of kit, be convenient to clinical other detection of going to bed, or patient uses at home voluntarily, avoid the shortcoming that sample may change in transportation, improve detection efficiency and the accuracy of anti-medicine antibody.
More than show and describe ultimate principle of the present utility model and principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; what describe in above-described embodiment just illustrates structure of the present utility model; under the prerequisite not departing from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall within the scope of claimed the utility model.Claimed scope of the present utility model is defined by appending claims and equivalent thereof.
Claims (1)
1. one kind is detected the kit of the anti-medicine antibody of EGFR antibody drug fast, it comprises box body and lid, it is characterized in that: bottom box body, be provided with the double-layer plastic holder measure-alike with box body, described plastic tray upper surface is provided with bottle holder, for placing acidolysis solution reagent bottle, neutralization solution reagent bottle and EDTA-K
2the detection bottle of anti-coagulants, described plastic tray is also provided with the test card slot placing EGFR antibody drug bag quilt, test card, described test card is made up of PVC board, the two ends of test card are respectively equipped with sample pad and absorption pad, middle part is provided with nitrocellulose filter detection layers, nitrocellulose filter detection layers and sample pad intersection, be provided with the gold mark pad of the EGFR antibody drug that bag is marked by gold, gold mark pad one end portion is arranged under sample pad, the other end is divided and is arranged on nitrocellulose filter detection layers, in the detection layers continued with sample pad, be provided with detection line and nature controlling line, detection line is coated with EGFR antibody drug, nature controlling line is coated with the positive antibody of anti-egfr antibodies medicine, the interlayer placing low temperature ice bag is also provided with in described lid and plastic tray.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520382279.4U CN205120720U (en) | 2015-06-05 | 2015-06-05 | Kit of anti medicine antibody of short -term test EGFR antibody medicine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520382279.4U CN205120720U (en) | 2015-06-05 | 2015-06-05 | Kit of anti medicine antibody of short -term test EGFR antibody medicine |
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| Publication Number | Publication Date |
|---|---|
| CN205120720U true CN205120720U (en) | 2016-03-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520382279.4U Active CN205120720U (en) | 2015-06-05 | 2015-06-05 | Kit of anti medicine antibody of short -term test EGFR antibody medicine |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047702A (en) * | 2016-08-15 | 2016-10-26 | 北京昱龙盛世生物科技有限公司 | Kit for culture of adipose-derived stem cells and culture method |
| CN106070185A (en) * | 2016-08-01 | 2016-11-09 | 北京世纪劲得生物技术有限公司 | A kind of for fat marrow collection suit |
| CN106680493A (en) * | 2017-01-21 | 2017-05-17 | 上海为然环保科技有限公司 | Agricultural product pesticide residual colloidal gold detection kit |
-
2015
- 2015-06-05 CN CN201520382279.4U patent/CN205120720U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106070185A (en) * | 2016-08-01 | 2016-11-09 | 北京世纪劲得生物技术有限公司 | A kind of for fat marrow collection suit |
| CN106070185B (en) * | 2016-08-01 | 2018-12-28 | 北京世纪劲得生物技术有限公司 | One kind is for fat marrow acquisition suit |
| CN106047702A (en) * | 2016-08-15 | 2016-10-26 | 北京昱龙盛世生物科技有限公司 | Kit for culture of adipose-derived stem cells and culture method |
| CN106047702B (en) * | 2016-08-15 | 2018-07-20 | 北京昱龙盛世生物科技有限公司 | A kind of kit and cultural method for cultivating fat stem cell |
| CN106680493A (en) * | 2017-01-21 | 2017-05-17 | 上海为然环保科技有限公司 | Agricultural product pesticide residual colloidal gold detection kit |
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| GR01 | Patent grant |