CN202164305U - Quick detection card for enterobacter sakazakii in dairy products - Google Patents
Quick detection card for enterobacter sakazakii in dairy products Download PDFInfo
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- CN202164305U CN202164305U CN2011202551373U CN201120255137U CN202164305U CN 202164305 U CN202164305 U CN 202164305U CN 2011202551373 U CN2011202551373 U CN 2011202551373U CN 201120255137 U CN201120255137 U CN 201120255137U CN 202164305 U CN202164305 U CN 202164305U
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- detection card
- enterobacter sakazakii
- dairy products
- rapid detection
- water absorption
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Abstract
The utility model relates to the field of food safety microorganism detection, in particular to a quick detection card for enterobacter sakazakii in dairy products, which is formed by water absorption filter paper and a water absorption gel which are adhered together. The water absorption filter paper contains a selective color development culture medium and antibiotics, a cover film provided with a label is adhered to the water absorption filter paper, and polystyrene (PP) synthetic paper is adhered under the water absorption gel. The quick detection card for enterobacter sakazakii in the dairy products has the advantages of being quick, economical, convenient and the like, is simple in structure, easy to operate, short in detection time and low in detection cost, and can improve working efficiency of dairy product detection by a large margin.
Description
Technical field
The utility model relates to food safety Micro biological Tests detection range, is specifically related to the rapid detection card of Enterobacter sakazakii in a kind of dairy products.
Background technology
Enterobacter sakazakii is newfound a kind of pathogenic bacterium in the dairy products; Distributed and the case broken out occurs in the whole world in succession by neonatal meningitis, septicemia and the necrotic colitis of its initiation, the mortality ratio that causes is higher, Enterobacter sakazakii is constantly taken place in recent years infect incident; By 2004; The all parts of the world report is diagnosed a disease example really more than 60 examples, causes a lot of commercial formulated infant milk pruinescences extensively to be recalled, and Enterobacter sakazakii has caused the attention of world many countries relevant departments.The Fuyang caused the powdered infant formulation incident inferior of " major part baby " to cause the great attention of the Chinese government in 2004, and from 87 parts of powdered infant formulations, was separated to 11 strain Enterobacter sakazakiis first, and recall rate reaches 12.6%.The World Food Programme in 2004 and the World Health Organization classify Enterobacter sakazakii and Salmonellas as baby formula milk powder category-A pathogenic bacterium jointly through the risk assessment.To the detection of Enterobacter sakazakii in the dairy products, be the important process content of food sanitation epidemic prevention, also be one of important indicator of dairy production enterprise inspection by oneself.
The detection method of existing Enterobacter sakazakii mainly contains traditional biochemical method, regular-PCR and fluorescence quantifying PCR method, does not see the report that has to the rapid detection card of Enterobacter sakazakii in the dairy products at present as yet.Existing detection method exists some defectives: the experiment that traditional biochemical method relates to is many, complex operation step, report assay time long, preparation and tailing in work are heavy; PCR method detecting instrument equipment price is expensive, needs to be equipped with special laboratory, and operational requirement is strict, and is high to reviewer's requirement, difficult the popularization.
The utility model content
The purpose of the utility model is according to the above-mentioned deficiency that exists in the prior art; The rapid detection card of Enterobacter sakazakii in a kind of dairy products is provided; To overcome the above-mentioned shortcoming of the existing detection technique of Enterobacter sakazakii in the dairy products, it is short to reach detection time, and it is low to detect cost; Simple and convenient purpose, thus scientific basis and directive function are provided for milk-product production and food safety.
The utility model above-mentioned purpose is achieved through following technical scheme:
The rapid detection card of Enterobacter sakazakii in a kind of dairy products is made up of with water absorbent gel 2 absorbent filter 1 that sticks on together, and said absorbent filter 1 contains selective coloration culture medium and microbiotic, and said water absorbent gel is pasted with PP synthetic paper 32 times.The conduct of PP synthetic paper 3 is as the carrier that carries absorbent filter and water absorbent gel, prevents the moisture turnover.
As a kind of preferred version, be pasted with epiphragma 4 on the said absorbent filter 1.The effect of epiphragma 4 is in order to form an excellent environment that helps microorganism growth, to prevent to pollute and make things convenient for the result to observe counting.
Further, above-mentioned epiphragma 4 is the polyester PET transparent film, and it is of a size of 90mm*80mm, and it is a waterproof and breathable, transparent, is printed on the film of counting lattice.
As a kind of preferred version, be pasted with label 5 on the said epiphragma 4.The effect of label 5 is for ease check sample to be carried out mark.
As preferred version further, said absorbent filter 1 is a non-woven fabrics; Said PP synthetic paper 3 sizes are 95mm*80mm; Said label 5 is an adhesive label, and it is of a size of 80mm*15mm.
Compared with prior art, the utlity model has following beneficial effect:
The utility model is simplified to step completion to traditional multistep culture identification process, and 24h can report the result, and has saved the plenty of time, and is convenient and swift; Save heavy preparation, tailing in work, volume is light and handy, is easy to carry ready access upon use; With respect to the method for pour plate, only need less substratum on every test card, thereby can reduce cost greatly; Simple to operate, sample can directly add, and need not special skill; Testing process does not need costliness or complicated instrument, helps applying in diary processing enterprise and monitoring department of basic unit, can increase substantially the dynamics and the efficient of food safety monitoring, for the rapid detection of Enterobacter sakazakii in the dairy products provides new approach.
Description of drawings
Fig. 1 is the structure schematic top plan view of the said test card of the utility model;
Fig. 2 is that the structure of the said test card of the utility model is analysed and observe enlarged diagram.
Embodiment
Come further to explain the utility model below in conjunction with embodiment, but embodiment does not do any type of qualification to the utility model.
Make the utility model with reference to Fig. 1,2.The rapid detection card of this dairy products Enterobacter sakazakii; It is characterized in that it is made up of absorbent filter 1 and water absorbent gel 2, absorbent filter 1 sticks on together with water absorbent gel 2 closely, selective color developing culture medium of immersion and microbiotic on the absorbent filter 1; As the carrier that carries absorbent filter 1 and water absorbent gel 2; Be pasted with one deck white PP synthetic paper 3 water absorbent gel 2 times through the glue layer that scribbles,, prevent to pollute and make things convenient for the result to observe counting in order to form an excellent environment that helps microorganism growth; On absorbent filter 1, be pasted with one deck waterproof and breathable, transparent, be printed on the counting lattice epiphragma 4; For ease check sample is carried out mark, on epiphragma 4, be pasted with a label 5, label 5, white PP synthetic paper 3 and epiphragma 4 are glued together.
The non-woven fabrics that described absorbent filter 1 is processed for the natural fiber cellulosic material, it is of a size of the square of length of side 62mm; Described white PP synthetic paper 3 length are 95mm, and width is 80mm; The corresponding glue layer of size of surface covered and absorbent filter on white PP synthetic paper 3; Be stained with and cover one deck water absorbent gel 2; Absorbent filter 1 is soaked in dissolved in selective coloration culture medium and the antibiotic nutrient solution, stick on after the taking-up on the water absorbent gel layer 2; Described epiphragma 4 is the polyester PET transparent film, and waterproof and breathable is transparent, also can be printed on the counting lattice, and length is 90mm, and width is 80mm.Said label 5 is an adhesive label, and length is 80mm, and width is 15mm; The selective coloration culture medium 11g that comprises Enterobacter sakazakii in the described nutrient solution, tryptose soya agar 2g, microbiotic 0.0035g; After test card is made, put into the gas sterilizer 3h that sterilizes, put into shady and cool lucifuge place and preserve subsequent use.
Realize through following process with the check of the utility model Enterobacter sakazakii in the dairy products.
(1) sample preparation: sample thief 25 mL (g) put into sampling jar or the homogeneous cup that contains 225 mL sterile salines, process the even liquid of sample of 1:10.
(2) inoculation: the rapid detection of these dairy products Enterobacter sakazakii is placed in smooth experiment table top; Open the upper strata epiphragma; With the even liquid of sterilization suction pipe absorption 2mL sample, slowly be added drop-wise to equably on the absorbent filter of test card central authorities, and then upper layer film is slowly covered down.Leave standstill the water absorbent gel layer is solidified, push gently in the middle of absorbent filter with finger, to drive bubble away.
(3) cultivate: the rapid detection card that will add appearance stacks, and the level of facing up places in the constant incubator, cultivates 15～24h down at 36 ℃ ± 1 ℃.
(4) interpretation as a result: rapid detection card is observed, and being glaucous bacterium colony is Enterobacter sakazakii.
Claims (2)
1. the rapid detection card of Enterobacter sakazakii in the dairy products is characterized in that being made up of the absorbent filter (1) and the water absorbent gel (2) that stick on together, is pasted with PP synthetic paper (3) under the said water absorbent gel (2).
2. rapid detection card according to claim 1 is characterized in that being pasted with on the said absorbent filter (1) epiphragma (4).
3. rapid detection card according to claim 2 is characterized in that being pasted with on the said epiphragma (4) label (5).
4. rapid detection card according to claim 1 is characterized in that the size of said absorbent filter (1) is 62*62mm
2
5. rapid detection card according to claim 1 is characterized in that the size of said PP synthetic paper (3) is 95mm*80mm.
6. rapid detection card according to claim 2 is characterized in that said epiphragma (4) is of a size of 90mm*80mm, and it is a waterproof and breathable, transparent, is printed on the film of counting lattice.
7. rapid detection card according to claim 3 is characterized in that said label (5) is of a size of 80mm*15mm.
8. rapid detection card according to claim 1 is characterized in that said absorbent filter (1) is a non-woven fabrics.
9. rapid detection card according to claim 7 is characterized in that said label (5) is an adhesive label.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202551373U CN202164305U (en) | 2011-07-19 | 2011-07-19 | Quick detection card for enterobacter sakazakii in dairy products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011202551373U CN202164305U (en) | 2011-07-19 | 2011-07-19 | Quick detection card for enterobacter sakazakii in dairy products |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202164305U true CN202164305U (en) | 2012-03-14 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011202551373U Expired - Lifetime CN202164305U (en) | 2011-07-19 | 2011-07-19 | Quick detection card for enterobacter sakazakii in dairy products |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202164305U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244670A (en) * | 2016-09-21 | 2016-12-21 | 广东达元绿洲食品安全科技股份有限公司 | A kind of Salmonella quickly detects culture medium and detection ware |
| CN106520542A (en) * | 2016-12-16 | 2017-03-22 | 天津科技大学 | Total bacterial count test piece and manufacturing method thereof |
| CN106754237A (en) * | 2016-11-21 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | A kind of microbe rapid test piece |
-
2011
- 2011-07-19 CN CN2011202551373U patent/CN202164305U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244670A (en) * | 2016-09-21 | 2016-12-21 | 广东达元绿洲食品安全科技股份有限公司 | A kind of Salmonella quickly detects culture medium and detection ware |
| CN106754237A (en) * | 2016-11-21 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | A kind of microbe rapid test piece |
| CN106520542A (en) * | 2016-12-16 | 2017-03-22 | 天津科技大学 | Total bacterial count test piece and manufacturing method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP03 | Change of name, title or address |
Address after: 902 room 7, No. 510663, 1st floor, science Avenue, hi tech Industrial Development Zone, Guangdong, Guangzhou Patentee after: Guangzhou Oasis Biochemistry Technology Co.,Ltd. Address before: 510000 room 506, building D, Guangzhou international business incubator, Science City, Guangzhou, Guangdong Patentee before: Guangzhou Oasis Biochemistry Technology Co., Ltd. |
|
| C56 | Change in the name or address of the patentee |
Owner name: GUANGDONG OASIS BIOCHEM FOOD SAFETY SCIENCE AND TE Free format text: FORMER NAME: GUANGZHOU OASIS BIOCHEMISTRY TECHNOLOGY CO., LTD. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 902 room 7, No. 510663, 1st floor, science Avenue, hi tech Industrial Development Zone, Guangdong, Guangzhou Patentee after: GUANGDONG DAYUAN OASIS FOOD SAFETY TECHNOLOGY CO., LTD. Address before: 902 room 7, No. 510663, 1st floor, science Avenue, hi tech Industrial Development Zone, Guangdong, Guangzhou Patentee before: Guangzhou Oasis Biochemistry Technology Co.,Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20120314 |