CN2908517Y - Test chip for pathogenic microorganism in food - Google Patents

Test chip for pathogenic microorganism in food Download PDF

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Publication number
CN2908517Y
CN2908517Y CN 200520120355 CN200520120355U CN2908517Y CN 2908517 Y CN2908517 Y CN 2908517Y CN 200520120355 CN200520120355 CN 200520120355 CN 200520120355 U CN200520120355 U CN 200520120355U CN 2908517 Y CN2908517 Y CN 2908517Y
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CN
China
Prior art keywords
food
testing
utility
organism
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CN 200520120355
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Chinese (zh)
Inventor
卢新
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangzhou Oasis Biochemistry Technology Co.,Ltd.
Original Assignee
卢新
蒋廉华
胡玥静
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 卢新, 蒋廉华, 胡玥静 filed Critical 卢新
Priority to CN 200520120355 priority Critical patent/CN2908517Y/en
Application granted granted Critical
Publication of CN2908517Y publication Critical patent/CN2908517Y/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Abstract

This is pathogenic microorganism testing paper for food, involving microbiological testing technology domain. The utility model consists of water-absorbing filter paper and selectivity chromogenic culture basic layer. Testing with this paper is better than the existing technology. The utility model is easy to operate. A large quantity of work, such as preparing culture medium, disinfection and cleaning the culture vessels is saved. The time of culturing pathogenic microorganism is saved. The sampling test can be operated at any time without the limit of environment, experimental equipment and technician, etc. The utility model can testing the intestinal bacteria group, escherichia coli, escherichia coli 0157, salmonella and staphylococcus aureus in food.

Description

The testing plate of pathogenic micro-organism in the food
(1) technical field
Technical field under the utility model is a microbiological Test, relates to a kind of quick test articles for use of pathogenic micro-organism in the food.
(2) background technology
Traditional pathogenic bacterium detection method has usually: the laboratory culture method---and the bacterium colony of cultivating on substratum usually approximately needs 2～9 day time; Immunoassay---based on antigen-antibody reaction, the result who obtained supposing in 24～48 hours; The molecules method---based on the DNA basis, can obtain accurate result in 8～48 hours.
The two kinds of methods in front, not only consuming time but also complicated.The detection speed of laboratory culture method is slow, and experimenter's operant level is had very high requirement.Method of immunity has nearly more than 20 years history till now, and it has certain advantage for traditional method, but it is based on the technology of phenotype analytical, and cross reaction causes false positive, and sensitivity is low relatively, usually the bacterium that increases that needs to do 1～2 day is cultivated more, just can reach the quantity of detection.The molecules method is difficult to visual inspection, detects relative complex, needs lab assistant that very strong operative technique is arranged.
In recent years, the product of Micro biological Tests in more domestic food mainly contains the mould and the saccharomycetic quick test scraps of paper that the micro-organism detecting plate that the patent No. is ZL 03226094.6 and the patent No. are ZL 03274715.2.These products mainly are the non-pathogenic microorganisms in the check food, and the bacterial classification of its check is more single, can not check out the pathogenic microorganism in the food.And, in these products in the past, can not indicate the place of check sample name and proving time.The utility model has all had tangible improvement aspect above-mentioned.
(3) summary of the invention
1, technical problem to be solved by this invention:
For solve described in the background technology to the existing problem in pathogenic micro-organism check aspect in the food, design a kind of easy and simple to handle, proving time is short, cost is low, highly sensitive, scope of survey is wide, can reflect the testing plate of sample name and proving time, be used for the food pathogenic micro-organism is carried out quick test the purpose of this utility model that Here it is.
2, the technical solution used in the present invention:
The utility model is made up of absorbent filter and the selective coloration culture medium layer that contains water absorbent gel, and absorbent filter and selective coloration culture medium layer closely are pasted together.In order to carry absorbent filter and to contain the selective coloration culture medium layer of water absorbent gel, prevent the moisture turnover, be preferably in the egative film that one deck waterproof and breathable is arranged below absorbent filter and the selective coloration culture medium layer, egative film is preferably made by synthetic materials.The selective coloration culture medium layer is bonded on the egative film, and absorbent filter is bonded on the selective coloration culture medium layer.In order to form an excellent environment that helps the pathogenic micro-organism growth, prevent to pollute and convenient the observation, being preferably in has one deck epiphragma on the absorbent filter, and epiphragma is waterproof and breathable, clear films preferably.In order to differentiate different check samples, be preferably in and be stained with a label on the epiphragma clear and definite proving time, preferably leave the hurdle of filling in of sample name and proving time on the label.
The utility model can be checked coliform, intestinal bacteria, colon bacillus 0157, Salmonellas and the streptococcus aureus in the food.
Realize by following process with the check of the utility model pathogenic micro-organism in the food.
1) the testing plate level is placed on the table top, opens epiphragma, draw sample stoste or diluent 0.4mL, evenly be added on the absorbent filter, gently epiphragma is put down then with the sterilization suction pipe.
2) push gently in the middle of absorbent filter with finger, make the moisture uniform distribution, and bubble is driven away.
3) testing plate that will add sample lies in 37 ℃ of incubators and cultivated 24 hours.
4) testing plate is observed, being mauve bacterium colony is pathogenic micro-organism; Be blue bacterium colony and be other microorganisms, non-pathogenic micro-organism.All testing plate that has the red-purple bacterium colony to occur, the sample of its check is positive; The bacterium colony that has only testing plate to present all is blue, and the sample of its check is just negative.
3, beneficial effect of the present invention:
Save a large amount of auxiliary works such as clean of preparation substratum, sterilization and cultivation vessel, can begin to carry out sampling Detection at any time, easy and simple to handle.Saved a large amount of incubation times, traditional multistep culture identification process be simplified to one the step finish, incubation time is shortened to 24 hours.Be not subjected to the restriction of many-sided conditions such as environment, experimental installation and technician.And the cost of check is low, helps applying in grass-roots unit and ordinary person, and the prevention that helps food detects.
(4) accompanying drawing and embodiment
Fig. 1 sees synoptic diagram according to the side of a kind of pattern (when raising epiphragma) that the utility model proposes.
Fig. 2 is a sectional view of the present utility model.
With reference to accompanying drawing, present embodiment is made up of following each several part:
1---represent label,
2---represent epiphragma,
3---represent absorbent filter,
4---representative contains the selective coloration culture medium layer of water absorbent gel,
5---represent egative film.
In the present embodiment: epiphragma 2 is waterproof and breathable, transparent film, and its length * wide size is 75 millimeters * 65 millimeters; Absorbent filter 3 is bonded on the selective coloration culture medium layer 4 that contains water absorbent gel, and their length * wide size all is 30 millimeters * 30 millimeters; The selective coloration culture medium layer 4 that contains water absorbent gel is bonded on the egative film 5; Egative film 5 is made waterproof and breathable by synthetic materials.Label 1, epiphragma 2 and egative film 5 are glued together.

Claims (5)

1, the testing plate of pathogenic micro-organism in a kind of food is characterized in that it is made up of absorbent filter and the selective coloration culture medium layer that contains water absorbent gel, and absorbent filter and selective coloration culture medium layer closely are pasted together.
2, the testing plate of pathogenic micro-organism in the food according to claim 1 is characterized in that absorbent filter and contains under the selective coloration culture medium layer of water absorbent gel being pasted with egative film.
3, the testing plate of pathogenic micro-organism in the food according to claim 1 is characterized in that absorbent filter and contains on the selective coloration culture medium layer of water absorbent gel being pasted with epiphragma.
4, the testing plate of pathogenic micro-organism in the food according to claim 3 is characterized in that epiphragma is waterproof and breathable, clear films.
5, the testing plate of pathogenic micro-organism in the food according to claim 3 is characterized in that label is arranged on the epiphragma.
CN 200520120355 2005-12-20 2005-12-20 Test chip for pathogenic microorganism in food Expired - Lifetime CN2908517Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200520120355 CN2908517Y (en) 2005-12-20 2005-12-20 Test chip for pathogenic microorganism in food

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200520120355 CN2908517Y (en) 2005-12-20 2005-12-20 Test chip for pathogenic microorganism in food

Publications (1)

Publication Number Publication Date
CN2908517Y true CN2908517Y (en) 2007-06-06

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200520120355 Expired - Lifetime CN2908517Y (en) 2005-12-20 2005-12-20 Test chip for pathogenic microorganism in food

Country Status (1)

Country Link
CN (1) CN2908517Y (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106244670A (en) * 2016-09-21 2016-12-21 广东达元绿洲食品安全科技股份有限公司 A kind of Salmonella quickly detects culture medium and detection ware
CN106754237A (en) * 2016-11-21 2017-05-31 百奥森（江苏）食品安全科技有限公司 A kind of microbe rapid test piece
CN109251957A (en) * 2018-10-17 2019-01-22 长春理工大学 Staphylococcus aureus selective coloration culture medium testing piece

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106244670A (en) * 2016-09-21 2016-12-21 广东达元绿洲食品安全科技股份有限公司 A kind of Salmonella quickly detects culture medium and detection ware
CN106754237A (en) * 2016-11-21 2017-05-31 百奥森（江苏）食品安全科技有限公司 A kind of microbe rapid test piece
CN109251957A (en) * 2018-10-17 2019-01-22 长春理工大学 Staphylococcus aureus selective coloration culture medium testing piece

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Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
C56 Change in the name or address of the patentee
CP03 Change of name, title or address

Address after: Guangdong, Guangzhou, Zhongshan road 189, new DA Shun Business Center, E building, five story north, zip: 510665

Co-patentee after: Jiang Lianhua

Patentee after: Lu Xin

Co-patentee after: Hu Yuejing

Address before: Room 308, west seat, Ting Li building, 491 Zhongshan Avenue West, Guangzhou, Guangdong, zip code: 510665

Co-patentee before: Jiang Lianhua

Patentee before: Lu Xin

Co-patentee before: Hu Yuejing

EE01 Entry into force of recordation of patent licensing contract

Assignee: Guangzhou Oasis Biochemistry Technology Co., Ltd.

Assignor: Luxin|Jiang Lianhua|Hu Yuejing

Contract fulfillment period: 2008.7.26 to 2015.12.19

Contract record no.: 2009440001015

Denomination of utility model: Test chip for pathogenic microorganism in food

Granted publication date: 20070606

License type: Exclusive license

Record date: 20090806

LIC Patent licence contract for exploitation submitted for record

Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2008.7.26 TO 2015.12.19; CHANGE OF CONTRACT

Name of requester: GUANGZHOU OASIS BIOCHEMISTRY TECHNOLOGY CO., LTD.

Effective date: 20090806

DD01 Delivery of document by public notice

Addressee: Hu Yuejing

Document name: Notification to Pay the Fees

ASS Succession or assignment of patent right

Free format text: FORMER OWNER: JIANG LIANHUA HU YUEJING

Effective date: 20120104

Owner name: GUANGZHOU OASIS BIOCHEMISTRY TECHNOLOGY CO., LTD.

Free format text: FORMER OWNER: LU XIN

Effective date: 20120104

C41 Transfer of patent application or patent right or utility model
COR Change of bibliographic data

Free format text: CORRECT: ADDRESS; FROM: 510665 GUANGZHOU, GUANGDONG PROVINCE TO: 510663 GUANGZHOU, GUANGDONG PROVINCE

TR01 Transfer of patent right

Effective date of registration: 20120104

Address after: 902 room 7, No. 510663, 1st floor, science Avenue, Guangzhou hi tech Industrial Development Zone, Guangdong, China

Patentee after: Guangzhou Oasis Biochemistry Technology Co.,Ltd.

Address before: 510665 Guangdong Province, Guangzhou Zhongshan Avenue E new daysun Business Center No. 189 North Building five layer

Co-patentee before: Jiang Lianhua

Patentee before: Lu Xin

Co-patentee before: Hu Yuejing

CX01 Expiry of patent term

Granted publication date: 20070606

EXPY Termination of patent right or utility model
DD01 Delivery of document by public notice

Addressee: Guangzhou Oasis Biochemistry Technology Co.,Ltd.

Document name: Notification of Expiration of Patent Right Duration