CN108285917A - Detect coliform and the chromogenic culture medium and detection lug of salmonella simultaneously - Google Patents
Detect coliform and the chromogenic culture medium and detection lug of salmonella simultaneously Download PDFInfo
- Publication number
- CN108285917A CN108285917A CN201711427655.7A CN201711427655A CN108285917A CN 108285917 A CN108285917 A CN 108285917A CN 201711427655 A CN201711427655 A CN 201711427655A CN 108285917 A CN108285917 A CN 108285917A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- chromogenic
- salmonella
- coliform
- bromo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
Abstract
The invention discloses coliform and the chromogenic culture medium and detection lug of salmonella is detected simultaneously, chromogenic culture medium contains peptone, yeast extract, beef extract powder, sodium chloride, Sodium Pyruvate, cholate, enzyme-specific chromogenic substrate, enzyme inducer, stabilizer, ovobiocin, superabsorbent gel polymer.Chromogenic culture medium of the present invention is used for while detecting coliform and salmonella, detection sensitivity is high, detection time is short, it is efficient, qualitative detection and quantitative detecting analysis can be carried out at the same time to coliform and salmonella, experimental implementation is significantly reduced, it is cost-effective, it can be applied to the places such as tableware direct sample detection, the detection of food processing environment equipment.Wherein, the aobvious blue bacterium point of coliform, the aobvious red bacterium point of salmonella.
Description
Technical field
The present invention relates to microorganism detection field, more particularly to colour developing that is a kind of while detecting coliform and salmonella
Culture medium and detection lug.
Background technology
Coliform is the leather of the aerobic and amphimicrobian of a kind of sour aerogenesis of energy lactose fermenters production under certain condition of culture
Lan Shi feminine gender sporeless bacteriums.Scientific research survey report shows that coliform is widely present in animal wastes and the water by its pollution
In the environment such as body, food production line.Coliform is examined the hygienic quality of food and its contact medium important as one
One of index.
Salmonella is a member in enterobacteriaceae lactobacteriaceae, is a kind of important micro- life of causing a disease that can lead to zoonosis
Object causes the example of food poisoning to be reported in media, therefore is pacified by various countries' food by edible by salmonella-polluted food
The attention of full supervision department.
It is polluted by salmonella or coliform and brings a large amount of economic loss, threaten human health and normal life
It is living.Two kinds of bacterium are detected in 4789 series standards of China GB at present and rely primarily on conventional culture dish method, using a series of biochemical trainings
Feature and serological Identification are supported to detect.GB 14934-2016 standards require coliform to microbiological indicator in disinfection Dining tool
Group and salmonella must not detect.
As the subject technologies such as immunology, biochemistry, molecular biology, zymetology, gene sequencing obtain tremendous development, use
In the constantly broadening of the detection means of salmonella and coliform and have the characteristics that quick, easy, height is special, such as RTPCR, glue
The rapid detection methods such as body gold immune test paper method, enzyme linked immunological fast detection method, DNA probe technology and quick sequencing technologies, just
It is widely applied in certain field.But these technologies have higher technology to require and mating make Micro biological Tests personnel
With reagent, expensive equipment, higher operating costs, some technologies are there is also immature not perfect, and there has been no the bases that base promotes.
Enzyme-specific, which is generated, using bacterium decomposes that specific chromogenic substrate is chromogenic to reach the chromogenic culture medium of Testing and appraisal purpose in state
It is interior to have preferably development, it is embedded into testing process using chromogenic culture medium as a kind of screening means in GB 4789.4, and GB
14934 coliform Rapid detecion paper methods are classified as a kind of detection method.But the chromogenic culture medium being commercialized on the market at present
Or manufactured paper disc, detection card mostly can only individually detect salmonella or coliform.
CN102827918A discloses a kind of salmonella color culture medium formula, and main component is：Agar, albumen
Peptone, beef extract powder, sodium chloride, surfactant, cholate, enzyme inducer, sad esterase chromogenic substrate, beta galactosidase colour developing
Substrate, hexosaminidase chromogenic substrate, ovobiocin composition.Salmonella strains are in aubergine typical case bacterium on the culture medium
It falls, not repressed part coliform is in blue-green bacterium colony, and part coliform Pseudomonas can be because be suppressed on the culture medium
And colour developing can not be grown.
CN106244670A discloses a kind of salmonella and quickly detects culture medium and detection ware, mainly solidifying by absorbing water
Glue, tryptone, beef extract powder, yeast extract, sodium chloride, cholate, specific chromogenic substrate, IPTG, ovobiocin and stabilization
Agent forms.
For existing chromogenic culture medium by the highly selective bacteriostatic agent of large dosage as bacteria screening condition, culture medium is unable to high temperature
It sterilizes and culture medium itself may occur and carry disease germs and false positive phenomenon occur, similar coliform chromogenic medium, Salmonella
The mutual inhibition or shortage of bacterium chromogenic culture medium and its Rapid detecion paper equivalent type product due to selective substances are corresponding
Enzyme-specific chromogenic substrate, enzyme inducer and cannot achieve the purpose that while detect.
Invention content
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of colour developings obviously, and detection result is more preferably same
When detect coliform and salmonella chromogenic culture medium and detection lug.
The technical solution used in the present invention is：
A kind of chromogenic culture medium that can detect salmonella and coliform simultaneously contains macromolecule per 1000mL culture mediums
2~50g of water absorbent gel, 5~30g of peptone, 1~10g of yeast extract, 1~10g of beef extract powder, 3~15g of sodium chloride, pyruvic acid
The chloro- 3- indoles of 0.001~15g of sodium, 2~15g of cholate, 2~40mg of ovobiocin, the bromo- 6- of 5- -0.1~0.8g of monooctyl ester, 5- is bromo-
0.1~0.8g of the chloro- 3- indoles-β-D- galactosides of 4-, 0.001~0.6g of IPTG, 1~16g of stabilizer.
As being further improved for above-mentioned chromogenic culture medium, contain per 1000mL culture mediums superabsorbent gel polymer 2~
40g, 10~25g of peptone, 2~8g of yeast extract, 2~8g of beef extract powder, 3~8g of sodium chloride, 0.001~10g of Sodium Pyruvate,
The chloro- chloro- 3- indoles-β-of 3- indoles-bromo- 4- of 0.1~0.6g of monooctyl ester, 5- of 2~8g of cholate, 2~30mg of ovobiocin, the bromo- 6- of 5-
0.1~0.6g of D- galactosides, 0.01~0.3g of IPTG, 2~10g of stabilizer.
As being further improved for above-mentioned chromogenic culture medium, contain per 1000mL culture mediums superabsorbent gel polymer 2~
30g, 10~20g of peptone, yeast extract 5g, 3~8g of beef extract powder, 3~6g of sodium chloride, 0.1~5g of Sodium Pyruvate, cholate 2
The chloro- 3- indoles-β-D- galas of the chloro- 3- indoles-bromo- 4- of 0.2~0.5g of monooctyl ester, 5- of~5g, 5~25mg of ovobiocin, the bromo- 6- of 5-
0.2~0.5g of glucosides, 0.1~0.3g of IPTG, 5~10g of stabilizer.
As being further improved for above-mentioned chromogenic culture medium, water absorbent gel is by polyvinyl alcohol, polyethylene oxide, acrylic acid tree
Fat, guar gum, sodium carboxymethylcellulose, agar form, and contain 1~10g of polyvinyl alcohol, polycyclic oxygen second in every 1000mL culture mediums
1~10g of alkane, 1~15g of acrylic resin, 1~15g of guar gum, 1~5g of sodium carboxymethylcellulose, 1~5g of agar.
As being further improved for above-mentioned chromogenic culture medium, contain 2~8g of polyvinyl alcohol, polycyclic in every 1000mL culture mediums
2~8g of oxidative ethane, 4~12g of acrylic resin, 2~10g of guar gum, 1~4g of sodium carboxymethylcellulose, 1~4g of agar.
As being further improved for above-mentioned chromogenic culture medium, contain 3~5g of polyvinyl alcohol, polycyclic in every 1000mL culture mediums
3~5g of oxidative ethane, 4~8g of acrylic resin, 4~6g of guar gum, 2~4g of sodium carboxymethylcellulose, 1~2g of agar.
As being further improved for above-mentioned chromogenic culture medium, stabilizer is made of SDS, tween, glycerine, is cultivated per 1000mL
Contain 0.05~0.5g of SDS, 2~8g of tween, 1~10g of glycerine in base.
As being further improved for above-mentioned chromogenic culture medium, 0.05~0.2g of SDS are contained in every 1000mL culture mediums, are spat
2~5g of temperature, 1~8g of glycerine.
As being further improved for above-mentioned chromogenic culture medium, contain 0.1~0.2g of SDS, tween in every 1000mL culture mediums
4g, 3~5g of glycerine.
A kind of color developing detection piece that can detect coliform and salmonella simultaneously, including absorption carrier, absorption carrier are negative
It is loaded with above-mentioned chromogenic culture medium.
The beneficial effects of the invention are as follows：
The coliform and salmonella color culture medium of the present invention, by formulation optimization, especially to the excellent of stabilizer
Change, while adding Sodium Pyruvate, compound stabilizer SDS, tween, glycerine and Sodium Pyruvate synergistic effect can promote damage thin
The fast quick-recovery of bacterium, adaptation inhibitor environment, and its own has preferably other enterobacteriaceaes outside coliform and salmonella
Inhibiting effect, the selectivity of basal culture medium can be improved, contribute to the dosage for reducing cholate, ovobiocin, reduce pair
Escherichia coli and Salmonella growth inhibiting effect.It is added to enzyme inducer simultaneously, to reach while detect two kinds of bacteriums
Purpose.Qualitative and quantitative testing is carried out to coliform and salmonella, colony characteristics are apparent, are easily recognized counting, detection week
Phase is short, save a large amount of manpower and materials, can be used for coliform in food and its production environment and salmonella is quickly examined
It surveys and Preliminary Identification, color developing detection piece can be used for tableware microbial rapid detection, law enforcement is facilitated to monitor.
The color developing detection piece specificity of the present invention is good, convenient to use, can detect coliform and Salmonella simultaneously
Two indexs of bacterium, detection cycle are short.This manufactured paper disc applies also for the microorganism detection in the places such as food processing environment.
Description of the drawings
Fig. 1 is the testing result photo of color developing detection piece 2；
Fig. 2 is the testing result photo of color developing detection piece 4.
Specific implementation mode
A kind of chromogenic culture medium that can detect salmonella and coliform simultaneously contains macromolecule per 1000mL culture mediums
2~50g of water absorbent gel, 5~30g of peptone, 1~10g of yeast extract, 1~10g of beef extract powder, 3~15g of sodium chloride, pyruvic acid
The chloro- 3- indoles of 0.001~15g of sodium, 2~15g of cholate, 2~40mg of ovobiocin, the bromo- 6- of 5- -0.1~0.8g of monooctyl ester, 5- is bromo-
0.1~0.8g of the chloro- 3- indoles-β-D- galactosides of 4-, 0.001~0.6g of IPTG, 1~16g of stabilizer.
As being further improved for above-mentioned chromogenic culture medium, contain per 1000mL culture mediums superabsorbent gel polymer 2~
40g, 10~25g of peptone, 2~8g of yeast extract, 2~8g of beef extract powder, 3~8g of sodium chloride, 0.001~10g of Sodium Pyruvate,
The chloro- chloro- 3- indoles-β-of 3- indoles-bromo- 4- of 0.1~0.6g of monooctyl ester, 5- of 2~8g of cholate, 2~30mg of ovobiocin, the bromo- 6- of 5-
0.1~0.6g of D- galactosides, 0.01~0.3g of IPTG, 2~10g of stabilizer.
As being further improved for above-mentioned chromogenic culture medium, contain per 1000mL culture mediums superabsorbent gel polymer 2~
30g, 10~20g of peptone, yeast extract 5g, 3~8g of beef extract powder, 3~6g of sodium chloride, 0.1~5g of Sodium Pyruvate, cholate 2
The chloro- 3- indoles-β-D- galas of the chloro- 3- indoles-bromo- 4- of 0.2~0.5g of monooctyl ester, 5- of~5g, 5~25mg of ovobiocin, the bromo- 6- of 5-
0.2~0.5g of glucosides, 0.1~0.3g of IPTG, 5~10g of stabilizer.
As being further improved for above-mentioned chromogenic culture medium, water absorbent gel is by polyvinyl alcohol, polyethylene oxide, acrylic acid tree
Fat, guar gum, sodium carboxymethylcellulose, agar form, and contain 1~10g of polyvinyl alcohol, polycyclic oxygen second in every 1000mL culture mediums
1~10g of alkane, 1~15g of acrylic resin, 1~15g of guar gum, 1~5g of sodium carboxymethylcellulose, 1~5g of agar.
As being further improved for above-mentioned chromogenic culture medium, contain 2~8g of polyvinyl alcohol, polycyclic in every 1000mL culture mediums
2~8g of oxidative ethane, 4~12g of acrylic resin, 2~10g of guar gum, 1~4g of sodium carboxymethylcellulose, 1~4g of agar.
As being further improved for above-mentioned chromogenic culture medium, contain 3~5g of polyvinyl alcohol, polycyclic in every 1000mL culture mediums
3~5g of oxidative ethane, 4~8g of acrylic resin, 4~6g of guar gum, 2~4g of sodium carboxymethylcellulose, 1~2g of agar.
As being further improved for above-mentioned chromogenic culture medium, stabilizer is made of SDS, tween, glycerine, is cultivated per 1000mL
Contain 0.05~0.5g of SDS, 2~8g of tween, 1~10g of glycerine in base.
As being further improved for above-mentioned chromogenic culture medium, 0.05~0.2g of SDS are contained in every 1000mL culture mediums, are spat
2~5g of temperature, 1~8g of glycerine.
As being further improved for above-mentioned chromogenic culture medium, contain 0.1~0.2g of SDS, tween in every 1000mL culture mediums
4g, 3~5g of glycerine.
A kind of color developing detection piece that can detect coliform and salmonella simultaneously, including absorption carrier, absorption carrier are negative
It is loaded with above-mentioned chromogenic culture medium.
Absorption carrier is absorption carrier commonly used in the art, such as filter paper.
With reference to embodiment, the technical solution that further illustrates the present invention.
The preparation method of detection lug is：Culture medium each component accurately is weighed by formula, is fully dissolved into two level water and makes
At culture solution, stabilizer and superabsorbent gel polymer are added under magnetic stirring, after mixing high-temperature sterilization, ovobiocin is used
It is added to and is cooled in 45~50 DEG C of culture solution after sterilizing filter filtration sterilization, quantitatively impregnate a certain amount of 5.0 × 5.0cm
Absorbent filter, is protected from light drying under gnotobasis, and aseptic packaging obtains.
Embodiment 1
A kind of chromogenic culture medium being used for while detecting salmonella and coliform contains poly- second per 1000mL culture mediums
Enol 3g, polyethylene oxide 3g, acrylic resin 4g, guar gum 5g, sodium carboxymethylcellulose 2g, agar 1g, peptone 10g,
The chloro- 3- Yin of yeast extract 5g, beef extract powder 3g, sodium chloride 4g, Sodium Pyruvate 3g, cholate 5g, the bromo- 6- of ovobiocin 15mg, 5-
Chloro- 3- indoles-β-D- galactosides (X-Gal) 0.3g of diindyl-bromo- 4- of monooctyl ester 0.2g, 5-, IPTG 0.1g, SDS 0.1g, tween
3g, glycerine 5g.
Embodiment 2
A kind of chromogenic culture medium being used for while detecting salmonella and coliform contains poly- second per 1000mL culture mediums
Enol 5g, polyethylene oxide 4g, acrylic resin 7g, guar gum 4g, sodium carboxymethylcellulose 2g, agar 1g, peptone 15g,
The chloro- 3- Yin of yeast extract 5g, beef extract powder 8g, sodium chloride 6g, Sodium Pyruvate 2g, cholate 3g, the bromo- 6- of ovobiocin 10mg, 5-
Chloro- 3- indoles-β-D- galactosides (X-Gal) 0.5g of diindyl-bromo- 4- of monooctyl ester 0.5g, 5-, IPTG 0.3g, SDS 0.2g, tween
5g, glycerine 3g.
Embodiment 3
A kind of chromogenic culture medium being used for while detecting salmonella and coliform contains poly- second per 1000mL culture mediums
Enol 4g, polyethylene oxide 3g, acrylic resin 6g, guar gum 4g, sodium carboxymethylcellulose 3g, agar 2g, peptone 20g,
The chloro- 3- Yin of yeast extract 5g, beef extract powder 4g, sodium chloride 4g, Sodium Pyruvate 0.5g, cholate 2g, the bromo- 6- of ovobiocin 5mg, 5-
Chloro- 3- indoles-β-D- galactosides (X-Gal) 0.25g of diindyl-bromo- 4- of monooctyl ester 0.2g, 5-, it IPTG 0.15g, SDS 0.2g, spits
Warm 5g, glycerine 4g.
Embodiment 4
A kind of chromogenic culture medium being used for while detecting salmonella and coliform contains poly- second per 1000mL culture mediums
Enol 4g, polyethylene oxide 3.5g, acrylic resin 5g, guar gum 5g, sodium carboxymethylcellulose 3g, agar 2g, peptone
10g, yeast extract 5g, beef extract powder 6g, sodium chloride 5g, Sodium Pyruvate 5g, cholate 3g, the bromo- 6- of ovobiocin 15mg, 5- are chloro-
Chloro- 3- indoles-β-D- galactosides (X-Gal) 0.4g of 3- indoles-bromo- 4- of monooctyl ester 0.3g, 5-, IPTG 0.2g, SDS 0.15g,
Tween 4g, glycerine 4g.
Comparative example 1
A kind of chromogenic culture medium being used for while detecting salmonella and coliform contains poly- second per 1000mL culture mediums
Enol 3g, polyethylene oxide 3g, acrylic resin 4g, guar gum 5g, sodium carboxymethylcellulose 2g, agar 1g, peptone 10g,
The chloro- 3- Yin of yeast extract 5g, beef extract powder 3g, sodium chloride 4g, Sodium Pyruvate 3g, cholate 5g, the bromo- 6- of ovobiocin 15mg, 5-
Chloro- 3- indoles-β-D- galactosides (X-Gal) 0.3g of diindyl-bromo- 4- of monooctyl ester 0.2g, 5-, IPTG 0.1g.
It uses the chromogenic culture medium of Examples 1 to 4 and comparative example 1 that color developing detection piece is made respectively, is denoted as colour developing inspection respectively
Survey piece 1~4 and contrasting detection piece 1.
The actual sample testing result photo of color developing detection piece 2 and color developing detection piece 4 is as depicted in figs. 1 and 2.It can see
Go out, salmonella shows clearly aubergine spot on culture medium, and Escherichia coli then show clearly bluish-green color spot
Point.
Influence of the stabilizer to the result that develops the color
By Escherichia coli ATCC 25922, Enterobacter sakazakii ATCC 51329,
Enterobacter cloacae ATCC 13047、C.albicans ATCC 10231、Salmonellatyphimurium
50,115 5 kinds of reference cultures of CMCC (B) are brought back to life respectively, and the standard bacteria suspension that debita spissitudo is fabricated to physiological saline is (a concentration of
102~103Cfu/mL), it is inoculated into respectively in comparative example 1 and three kinds of embodiment 1, salmonella color culture medium culture mediums, 36 DEG C
Culture 18~for 24 hours.The results are shown in Table 1 for observation.
The colour developing result of table 1, different chromogenic culture mediums
As can be seen from Table 1：
1) the sensitive coliform bacterial strain in part cannot be detected by being not added with the comparative example 1 of stabilizer, be in candida albicans
False positive detects；
2) commercialization salmonella color culture medium cannot detect part coliform bacterial strain or be given birth on its culture medium
Length is suppressed, is detected in false positive to candida albicans；
3) be added to stabilizer embodiment 1 can above-mentioned coliform strain at detection well, dialogue vacation silk ferment
Mother does not detect, and coliform and salmonella are detected while capable of achieving the purpose that fine.
As it can be seen that the SDS, tween, glycerine in stabilizer have inhibition to the non-coliform strain in part and grampostive bacteria
Effect, and have the facilitations such as promotion damage bacterium restoration ecosystem to the coliforms strain such as salmonella, escherichia coli, it can
To increase the specificity of chromogenic culture medium well so that testing result is more accurate.
Specificity experiments
By Escherichia coli ATCC 25922, CitrobacterfreundiiATCC43864, S.faecalis
ATCC 29212、S.typhimurium ATCC 14028、Enterobacter sakazakii ATCC 51329、
Enterobacter cloacae ATCC 13047、S.ehteritidis CMCC(B)50335、
Salmonellatyphimurium CMCC(B)50115、Escherichia coli O157:H7ATCC 35150、
Staphyloccocus aureus Rosenbach CMCC(B)26003、Vibrio parahaemolyticus ATCC
17802、Bacillus cereus CMCC 63303、Moniliaalbican ATCC 10231、C.albicans ATCC
10231 equal 14 kinds of standard strains are brought back to life respectively, and the standard bacteria suspension (a concentration of 10 of debita spissitudo is fabricated to physiological saline2~
103Cfu/mL), it is inoculated into 4 chromogenic culture medium of embodiment, color developing detection piece 4, salmonella color culture medium, coliform respectively
On group's chromogenic culture medium, 36 DEG C of culture 15-24h.
Testing result record is as shown in table 2：
Table 2,14 kind of reference culture specificity experiments
As shown in Table 2, testing result of each reference culture on 4 chromogenic culture medium of embodiment and color developing detection piece 4 is complete
Unanimously；And salmonella color culture medium or coliform chromogenic medium, which is commercialized, the bacterial strain that cannot be detected.This hair
Bright chromogenic culture medium and detection lug specificity are good, can be used for detecting coliform and salmonella simultaneously.
The simulated experiment of coliform and salmonella in natural sample
The bacteria suspension (a concentration of 10 of suitable concentration is fabricated to after Escherichia coli and salmonella reference culture are brought back to life2~
103Cfu/mL), 1mL is taken uniformly to be applied to 50cm above plate to be detected after mixing2Analog sample is made in surface at area.It presses
It is sampled according to GB 14934-2016 standards：It is affixed on painting after color developing detection piece in embodiment is moistened with sterile saline
Have and removed after 30s on the sample of bacteria suspension, sets in sterile PE culture bags and observed after culture 15-24h；After respectively taking 1mL to mix simultaneously
Bacterial suspension inoculation to the chromogenic culture medium in NA tablets and embodiment on, observed after setting constant incubator culture 18-24h.
It is detected respectively with the chromogenic culture medium of color developing detection piece 2~4 and embodiment 2~4,2 testing result of embodiment
3 are shown in Table, 3 testing result of embodiment is shown in Table 4, and 4 testing result of embodiment is shown in Table 5, and the testing result of color developing detection piece 2~4 is shown in Table
6。
Table 3,2 testing result of embodiment
Table 4,3 testing result of embodiment
Table 5,4 testing result of embodiment
The testing result of table 6, color developing detection piece 2~4
The chromogenic culture medium specificity of the present invention known to table 3~5 is good, and accuracy is high, can be used for detecting coliform simultaneously
And salmonella；Color developing detection piece of the present invention can detect coliform and salmonella simultaneously as shown in Table 6, and culture effect is good,
Coliform and salmonella can accurately be told.
Claims (10)
1. a kind of chromogenic culture medium that can detect salmonella and coliform simultaneously contains macromolecule suction per 1000mL culture mediums
2~50g of hydrogel, 5~30g of peptone, 1~10g of yeast extract, 1~10g of beef extract powder, 3~15g of sodium chloride, Sodium Pyruvate
0.001~15g, 2~15g of cholate, 2~40mg of ovobiocin, the bromo- 6- of 5- chloro- 3- indoles-bromo- 4- of 0.1~0.8g of monooctyl ester, 5-
0.1~0.8g of chloro- 3- indoles-β-D- galactosides, 0.001~0.6g of IPTG, 1~16g of stabilizer.
2. chromogenic culture medium according to claim 1, it is characterised in that：It is solidifying to contain macromolecule water uptake per 1000mL culture mediums
2~40g of glue, 10~25g of peptone, 2~8g of yeast extract, 2~8g of beef extract powder, 3~8g of sodium chloride, Sodium Pyruvate 0.001
The chloro- chloro- 3- Yin of 3- indoles-bromo- 4- of 0.1~0.6g of monooctyl ester, 5- of~10g, 2~8g of cholate, 2~30mg of ovobiocin, the bromo- 6- of 5-
0.1~0.6g of diindyl-β-D- galactosides, 0.01~0.3g of IPTG, 2~10g of stabilizer.
3. chromogenic culture medium according to claim 1, it is characterised in that：It is solidifying to contain macromolecule water uptake per 1000mL culture mediums
2~30g of glue, 10~20g of peptone, yeast extract 5g, 3~8g of beef extract powder, 3~6g of sodium chloride, 0.1~5g of Sodium Pyruvate,
The chloro- chloro- 3- indoles-β-of 3- indoles-bromo- 4- of 0.2~0.5g of monooctyl ester, 5- of 2~5g of cholate, 5~25mg of ovobiocin, the bromo- 6- of 5-
0.2~0.5g of D- galactosides, 0.1~0.3g of IPTG, 5~10g of stabilizer.
4. according to the chromogenic culture medium described in claims 1 to 3 any one, it is characterised in that：Water absorbent gel by polyvinyl alcohol,
Polyethylene oxide, acrylic resin, guar gum, sodium carboxymethylcellulose, agar form, and contain poly- second in every 1000mL culture mediums
1~10g of enol, 1~10g of polyethylene oxide, 1~15g of acrylic resin, 1~15g of guar gum, sodium carboxymethylcellulose 1~
5g, 1~5g of agar.
5. according to the chromogenic culture medium described in claims 1 to 3 any one, it is characterised in that：Contain in per 1000mL culture mediums
There are 2~8g of polyvinyl alcohol, 2~8g of polyethylene oxide, 4~12g of acrylic resin, 2~10g of guar gum, sodium carboxymethylcellulose 1
~4g, 1~4g of agar.
6. according to the chromogenic culture medium described in claims 1 to 3 any one, it is characterised in that：Contain in per 1000mL culture mediums
Have 3~5g of polyvinyl alcohol, 3~5g of polyethylene oxide, 4~8g of acrylic resin, 4~6g of guar gum, sodium carboxymethylcellulose 2~
4g, 1~2g of agar.
7. according to the chromogenic culture medium described in claims 1 to 3 any one, it is characterised in that：Stabilizer is by SDS, tween, sweet
Oil forms, and contains 0.05~0.5g of SDS, 2~8g of tween, 1~10g of glycerine in every 1000mL culture mediums.
8. chromogenic culture medium according to claim 7, it is characterised in that：In per 1000mL culture mediums containing SDS 0.05~
0.2g, 2~5g of tween, 1~8g of glycerine.
9. chromogenic culture medium according to claim 7, it is characterised in that：In per 1000mL culture mediums containing SDS 0.1~
0.2g, tween 4g, 3~5g of glycerine.
10. a kind of color developing detection piece that can detect coliform and salmonella simultaneously, including absorption carrier, it is characterised in that：
Absorption carrier loads 1~9 any one of them chromogenic culture medium of requirement of having the right.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711427655.7A CN108285917A (en) | 2017-12-26 | 2017-12-26 | Detect coliform and the chromogenic culture medium and detection lug of salmonella simultaneously |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711427655.7A CN108285917A (en) | 2017-12-26 | 2017-12-26 | Detect coliform and the chromogenic culture medium and detection lug of salmonella simultaneously |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108285917A true CN108285917A (en) | 2018-07-17 |
Family
ID=62832152
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711427655.7A Pending CN108285917A (en) | 2017-12-26 | 2017-12-26 | Detect coliform and the chromogenic culture medium and detection lug of salmonella simultaneously |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108285917A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110438051A (en) * | 2019-08-29 | 2019-11-12 | 海南省妇幼保健院 | A method of producing the Salmonella strains of high yield |
| CN111304280A (en) * | 2020-02-26 | 2020-06-19 | 成都海关技术中心 | Culture medium for color development detection of coliform group in food and seasoning containing pepper |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195086A (en) * | 2014-08-27 | 2014-12-10 | 博奥生物集团有限公司 | Composite enrichment medium for five bacteria and preparation method for composite enrichment medium |
| CN104673876A (en) * | 2015-02-09 | 2015-06-03 | 广州绿洲生化科技股份有限公司 | Transparent water-absorbing gel for microbial detection and detection plate |
| US9518283B1 (en) * | 2012-02-27 | 2016-12-13 | Paradigm Diagnostics, Inc. | Selective enrichment media and uses thereof |
| CN106244670A (en) * | 2016-09-21 | 2016-12-21 | 广东达元绿洲食品安全科技股份有限公司 | A kind of Salmonella quickly detects culture medium and detection ware |
| CN106591421A (en) * | 2017-02-27 | 2017-04-26 | 新疆农业大学 | Escherichia coli and/or salmonella and/or listeria monocytogenes enzyme production co-culture medium |
| CN206706106U (en) * | 2017-05-08 | 2017-12-05 | 广州聚佰生物科技有限公司 | Microorganism testing slice |
| HUE034396T2 (en) * | 2011-07-12 | 2018-02-28 | Foodchek Systems Inc | Culture medium, method for culturing salmonella and e. coli and method for detecting salmonella and e. coli |
-
2017
- 2017-12-26 CN CN201711427655.7A patent/CN108285917A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HUE034396T2 (en) * | 2011-07-12 | 2018-02-28 | Foodchek Systems Inc | Culture medium, method for culturing salmonella and e. coli and method for detecting salmonella and e. coli |
| US9518283B1 (en) * | 2012-02-27 | 2016-12-13 | Paradigm Diagnostics, Inc. | Selective enrichment media and uses thereof |
| CN104195086A (en) * | 2014-08-27 | 2014-12-10 | 博奥生物集团有限公司 | Composite enrichment medium for five bacteria and preparation method for composite enrichment medium |
| CN104673876A (en) * | 2015-02-09 | 2015-06-03 | 广州绿洲生化科技股份有限公司 | Transparent water-absorbing gel for microbial detection and detection plate |
| CN106244670A (en) * | 2016-09-21 | 2016-12-21 | 广东达元绿洲食品安全科技股份有限公司 | A kind of Salmonella quickly detects culture medium and detection ware |
| CN106591421A (en) * | 2017-02-27 | 2017-04-26 | 新疆农业大学 | Escherichia coli and/or salmonella and/or listeria monocytogenes enzyme production co-culture medium |
| CN206706106U (en) * | 2017-05-08 | 2017-12-05 | 广州聚佰生物科技有限公司 | Microorganism testing slice |
Non-Patent Citations (3)
| Title |
|---|
| RUBY M.LEE等: "Optimal pyruvate concentration for the recovery of Coliforms form food and water", 《JOURNAL OF FOOD PROTECTION》 * |
| YUTA MORISHIGE等: "Differential resuscitative effect of pyruvate and its analogues on VBNC(Viable but non-culturable)Salmonella", 《MICROBES ENVIRON》 * |
| 高涛等: "《药剂学》", 31 May 2017, 延边大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110438051A (en) * | 2019-08-29 | 2019-11-12 | 海南省妇幼保健院 | A method of producing the Salmonella strains of high yield |
| CN111304280A (en) * | 2020-02-26 | 2020-06-19 | 成都海关技术中心 | Culture medium for color development detection of coliform group in food and seasoning containing pepper |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Domig et al. | Methods used for the isolation, enumeration, characterisation and identification of Enterococcus spp.: 1. Media for isolation and enumeration | |
| CN103282513B (en) | For detecting goods and the method for target microorganism | |
| CN102311990B (en) | Chromogenic medium of coliform group and quick detection card thereof | |
| JP6938151B2 (en) | Built-in anaerobic environment generation culture device and usage | |
| JPH04504802A (en) | Precipitation test for microorganisms | |
| JP6920212B2 (en) | Culture device for anaerobic microorganisms | |
| JPH072119B2 (en) | Test set for measuring antibiotics in milk and method for measuring antibiotics in milk | |
| CN104611403A (en) | Food and tableware coliform bacteria rapid detection scrip preparation and application | |
| JP6448818B2 (en) | Culture device for lactic acid bacteria | |
| CN103290094A (en) | Staphylococcus aureus chromogenic medium and test piece thereof | |
| CN101186891A (en) | Salmonella color culture medium, detection kit an detection method | |
| CN108285917A (en) | Detect coliform and the chromogenic culture medium and detection lug of salmonella simultaneously | |
| CN101402989A (en) | Syringe-shaped microorganism culture device | |
| CN101970682A (en) | Method for detecting and/or identifying clostridium difficile | |
| CN105132519A (en) | Selective medium used for quantitative detection of escherichia coli and escherichia coli quantitative detection method | |
| CN111088318A (en) | Method for detecting bacterial drug sensitivity by TTC reduction reaction | |
| CN101413872B (en) | Method for rapidly detecting microorganism viable bacteria number | |
| CN105779564A (en) | Rapid detection method of escherichia coli in food | |
| CN101440391A (en) | Primer and probe sequence for multifluorescent PCR synchronous detection of Salmonella, Vibrio parahaemolyticus and Escherichia coli O157:H7 | |
| CN102146429A (en) | Vibrio alginolyticus selectivity differential medium | |
| CN106086159B (en) | A kind of zymolyte culture medium that can detect two kinds of fecal pollution indicator bacterias simultaneously and its application | |
| CN107287275B (en) | Culture medium, kit containing culture medium and application of culture medium | |
| Brown et al. | Automated detection of micro-organisms in blood cultures by means of the Malthus Microbiological Growth Analyser. | |
| CN203117162U (en) | Integrated membrane biosensor for quickly detecting escherichia coli | |
| Chai et al. | A portable optical sensor based on a one-off test strip for fast evaluation of bacterial contamination in raw tofu |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180717 |