CN202256339U - Kit for detecting echinococcosis granulose of animals - Google Patents
Kit for detecting echinococcosis granulose of animals Download PDFInfo
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- CN202256339U CN202256339U CN2011203990912U CN201120399091U CN202256339U CN 202256339 U CN202256339 U CN 202256339U CN 2011203990912 U CN2011203990912 U CN 2011203990912U CN 201120399091 U CN201120399091 U CN 201120399091U CN 202256339 U CN202256339 U CN 202256339U
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- echinococcosis granulosa
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Abstract
The utility model relates to a kit, in particular to a kit for detecting echinococcosis granulose of animals, which comprises a reaction box, a gold standard solution bottle and a scrubbing solution bottle. The reaction box comprises a box body, a box cover and a water absorption pad arranged in the box body. A sample feeding hole is arranged on the box cover, and a microfiltration membrane matched with the size of the hole is arranged right below the sample feeding hole and is placed on the water absorption pad.
Description
Technical field
The utility model relates to a kind of kit, is specifically related to a kind of animal echinococcosis granulosa detection kit.
Background technology
Echinococcosis granulosa; Claim cystic echinococcosis (Cystic echinococcosis again; CE), be that middle silk ribbon phase tapeworm by Echinococcus granulosus (Echinococcus granulosus) parasitizes a kind of serious harm health that causes in the people, animal body and the zoonosis of animal husbandry development for a long time.Confirmed that at present more than 30 province of China, autonomous region all have Echinococcus granulosus to distribute, the total trouble poultry in the whole nation surpasses 40,000,000, and annual New Development is suffered from about 10,000,000 of poultrys, causes economic loss to reach tens00000000 yuan.
At present, the detection of animal hydatidosis mainly adopts IHA, ELISA etc. illegal, and these methods have good sensitivity and specificity.But these methods need instrument and equipment, and application inconvenience is not suitable in the Qinghai-Tibet Platean especially and pastoral area, northwest basic unit applies.Therefore, a kind of kit that can detect the animal echinococcosis granulosa easily and efficiently becomes the emphasis of this area research.At present, do not see that the relevant report that can detect the kit of animal echinococcosis granulosa is easily and efficiently arranged.
The utility model content
The utility model technical matters to be solved provides a kind of kit that can fast detecting animal echinococcosis granulosa.
The utility model animal echinococcosis granulosa detection kit; Comprise reaction box; Gold mark liquid bottle and Washing liquid bottle, said reaction box comprises box body, lid and is positioned over the adsorptive pads in the box body that said lid is provided with well; Be provided with the miillpore filter that adapts with hole size under the well, miillpore filter is positioned on the adsorptive pads.
Wherein, the utility model animal echinococcosis granulosa detection kit, said well is preferably disposed on the central authorities of lid.
Further, said well is preferably the circular hole of diameter 0.4～0.8cm.
Wherein, the utility model animal echinococcosis granulosa detection kit, described miillpore filter can be preferably nitrocellulose filter for the miillpore filter of routine.
Wherein, the utility model animal echinococcosis granulosa detection kit also comprises antibody liquid bottle of animal particulate echinococcus or the antigen liquid bottle of animal particulate echinococcus.
The utility model animal echinococcosis granulosa detection kit can detect animal fast and whether suffer from echinococcosis granulosa, and it is easy to carry, does not need other instrument and equipments.The utility model is that the detection of animal echinococcosis granulosa provides a kind of new approach.
Description of drawings
Fig. 1 is the reaction box structural representation of the utility model animal echinococcosis granulosa detection kit, and mark 1 is a box body among the figure, and 2 is lid, and 3 is well, and 4 is miillpore filter, and 5 is adsorptive pads.
Embodiment
The utility model animal echinococcosis granulosa detection kit; Comprise reaction box; Gold mark liquid bottle and Washing liquid bottle, said reaction box comprises box body (1), lid (2) and is positioned over the adsorptive pads (5) in the box body (1) that said lid (2) is provided with well (3); Be provided with the miillpore filter (4) that adapts with hole size under the well (3), miillpore filter (4) is positioned on the adsorptive pads (5).
Wherein, the utility model animal echinococcosis granulosa detection kit, said well (3) is arranged at the central authorities of lid (2).
Further, said well (3) is preferably the circular hole of diameter 0.4～0.8cm.
Wherein, the utility model animal echinococcosis granulosa detection kit, described miillpore filter (4) is preferably nitrocellulose filter.
Wherein, the utility model animal echinococcosis granulosa detection kit also comprises antibody liquid bottle of animal particulate echinococcus or the antigen liquid bottle of animal particulate echinococcus.When detecting antigen, select the antibody liquid bottle of animal particulate echinococcus for use; Accordingly, when detecting antibody, then select the antigen liquid bottle of animal particulate echinococcus for use.The antigen liquid of antibody liquid of said animal particulate echinococcus or animal particulate echinococcus all can adopt conventional method to prepare.
Adopting the utility model animal echinococcosis granulosa detection kit, is example in the detection animal blood whether echinococcosis granulosa antigen is arranged, and can operate according to following steps:
A, drip the antibody liquid of animal particulate echinococcus in the diaphragm of nitrocellulose filter central authorities, making antibody liquid is that film is adsorbed;
B, reaction box is kept flat, in well (3), drip 1～2 of animal blood serum to be detected, it is infiltrated fully;
C, in well (3), drip gold mark liquid (being immune Au composite) 1～2, it is infiltrated fully;
D, in well (3), drip 2～3 of cleansing solutions, it is infiltrated fully;
Pale red spot shower is judged to positive reaction if e has clearly in film central authorities; Otherwise, then negative reaction.The depth of spot colour generation is correspondingly pointed out positive intensity.
The principle of the utility model is: with the miillpore filter is carrier, utilizes the filterability of miillpore filter, makes antigen-antibody reaction and washing go up the mode of oozing filtration membrane with liquid at a special percolating device (being reaction box) and accomplishes rapidly.Thereafter the gold mark liquid that adds also combines with the antigen that is combined on the film in diafiltration, and because of collaurum itself takes on a red color, positive reaction is promptly at film central authorities exhibit red spot.
Below in conjunction with embodiment the embodiment of the utility model is done further description, therefore the utility model is not limited among the described scope of embodiments.
As shown in Figure 1; The utility model animal echinococcosis granulosa detection kit comprises reaction box, gold mark liquid bottle and Washing liquid bottle; Said reaction box comprises box body (1), lid (2) and is positioned over the adsorptive pads (5) in the box body (1); Said lid (2) is provided with well (3), is provided with the miillpore filter (4) that adapts with hole size under the well (3), and miillpore filter (4) is positioned on the adsorptive pads (5).Said well (3) is arranged at the central authorities of lid (2).Said well (3) is the circular hole of diameter 0.4～0.8cm.Described miillpore filter (4) is a nitrocellulose filter.
Claims (5)
1. animal echinococcosis granulosa detection kit; Comprise reaction box; Gold mark liquid bottle and Washing liquid bottle, it is characterized in that: said reaction box comprises box body (1), lid (2) and is positioned over the adsorptive pads (5) in the box body (1) that said lid (2) is provided with well (3); Be provided with the miillpore filter (4) that adapts with hole size under the well (3), miillpore filter (4) is positioned on the adsorptive pads (5).
2. animal echinococcosis granulosa detection kit according to claim 1 is characterized in that: said well (3) is arranged at the central authorities of lid (2).
3. animal echinococcosis granulosa detection kit according to claim 1 and 2 is characterized in that: said well (3) is the circular hole of diameter 0.4～0.8cm.
4. animal echinococcosis granulosa detection kit according to claim 1 and 2 is characterized in that: said animal echinococcosis granulosa detection kit also comprises antibody liquid bottle of animal particulate echinococcus or the antigen liquid bottle of animal particulate echinococcus.
5. animal echinococcosis granulosa detection kit according to claim 3 is characterized in that: said animal echinococcosis granulosa detection kit also comprises antibody liquid bottle of animal particulate echinococcus or the antigen liquid bottle of animal particulate echinococcus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203990912U CN202256339U (en) | 2011-10-19 | 2011-10-19 | Kit for detecting echinococcosis granulose of animals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203990912U CN202256339U (en) | 2011-10-19 | 2011-10-19 | Kit for detecting echinococcosis granulose of animals |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202256339U true CN202256339U (en) | 2012-05-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011203990912U Expired - Fee Related CN202256339U (en) | 2011-10-19 | 2011-10-19 | Kit for detecting echinococcosis granulose of animals |
Country Status (1)
| Country | Link |
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| CN (1) | CN202256339U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104651328A (en) * | 2014-12-31 | 2015-05-27 | 中国疾病预防控制中心寄生虫病预防控制所 | Novel positive antigen of echinococcus granulosus and application of antigen |
| CN105641716A (en) * | 2016-01-11 | 2016-06-08 | 王思博 | Imaging model in small animal living bodies with echinococcus granulosus and construction method thereof |
| CN106198979A (en) * | 2016-07-14 | 2016-12-07 | 四川农业大学 | The application of Echinococcus granulosus dihydrofolate reductase |
| CN106198978A (en) * | 2016-07-14 | 2016-12-07 | 四川农业大学 | The application of Echinococcus granulosus leucine aminopeptidase |
-
2011
- 2011-10-19 CN CN2011203990912U patent/CN202256339U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104651328A (en) * | 2014-12-31 | 2015-05-27 | 中国疾病预防控制中心寄生虫病预防控制所 | Novel positive antigen of echinococcus granulosus and application of antigen |
| CN105641716A (en) * | 2016-01-11 | 2016-06-08 | 王思博 | Imaging model in small animal living bodies with echinococcus granulosus and construction method thereof |
| CN106198979A (en) * | 2016-07-14 | 2016-12-07 | 四川农业大学 | The application of Echinococcus granulosus dihydrofolate reductase |
| CN106198978A (en) * | 2016-07-14 | 2016-12-07 | 四川农业大学 | The application of Echinococcus granulosus leucine aminopeptidase |
| CN106198979B (en) * | 2016-07-14 | 2018-02-23 | 四川农业大学 | The application of Echinococcus granulosus dihyrofolate reductase |
| CN106198978B (en) * | 2016-07-14 | 2018-04-17 | 四川农业大学 | The application of Echinococcus granulosus leucine aminopeptidase |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120530 Termination date: 20181019 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |