CN201876459U - Trilink detecting card of estradiol, trihydroxyoestrin and diethylstilbestrol - Google Patents
Trilink detecting card of estradiol, trihydroxyoestrin and diethylstilbestrol Download PDFInfo
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- CN201876459U CN201876459U CN2010206129441U CN201020612944U CN201876459U CN 201876459 U CN201876459 U CN 201876459U CN 2010206129441 U CN2010206129441 U CN 2010206129441U CN 201020612944 U CN201020612944 U CN 201020612944U CN 201876459 U CN201876459 U CN 201876459U
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- diethylstilbestrol
- estradiol
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- estriol
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Abstract
The utility model discloses a trilink detecting card of estradiol, trihydroxyoestrin and diethylstilbestrol, which belongs to the field of oestrogens animal remedy residual detecting technologies. The trilink detecting card disclosed by the utility model is provided with a test strip in a casing, wherein the test strip comprises a sample pad, a goldcolloid membrane, a cellulose nitrate membrane and a water absorbing membrane which are sequentially stuck on a supporting backboard. The goldcolloid membrane is a fiberglass membrane which contains the goldcolloid markers of anti-estradiol antibody, anti-trihydroxyoestrin antibody and anti-diethylstilbestrol antibody. Three detecting strips which contain estradiol protein conjugate, trihydroxyoestrin protein conjugate and diethylstilbestrol protein conjugate respectively are arranged on the cellulose nitrate membrane. The cellulose nitrate membrane is also provided with another quality control strip which contains anti-rabbit antibody or anti-mouse antibody. The utility model has the advantages that the estradiol, trihydroxyoestrin and diethylstilbestrol contained in urine or feeding stuff, animal tissues, meat and liver can be detected at the same time. The detecting card is easy to prepare, saves detection cost, is convenient and quick to use, and has accurate results.
Description
Technical field
The present invention relates to a kind of utensil that detects the estrogens veterinary drug, particularly relate to the disposal route of a kind of estradiol, estriol and diethylstilbestrol three test card and test sample thereof, belong to estrogens detection of veterinary drugs in food technical field.
Background technology
The human environmental pollution that the animal incretion interferent is caused and the common cognition and the systematic study of harm are less than the history in 10 years, but more and more studies show that, having pollution that the continuous enrichment of the interfering pollutant of endocrine caused gives human and wild animal brings harm just by all means, and its natural water is worldwide extensively detected in urban sewage treatment system and the water system.
Wherein even more serious with the female hormone pollution especially, the present most widely used diethylstilbestrol that mainly contains estradiol, estriol and synthetic in the natural hormone.
Estradiol is the principal ingredient of natural estrogen, because of it can promote growth of animal and improve livestock and poultry lean meat ratio, and application is arranged in animal husbandry.It can be residual in animal body; be detrimental to health by food chain; Female Children is grown in advance; the male children breast tissue is grown womanlike; male reproductive system is changed unusually, causes pathology, and causes women with breast cancer and endometriosis incidence to rise; and have obvious carcinogenicity, developed countries such as America and Europe ban use of estradiol as feed addictive in succession.
Estriol is a kind of steroid hormone that extensively is present in the humans and animals body, detecting the particularly content of this hormone in the animal foodstuff of food, ensures that there are important meaning and effect in food hygiene and human health aspect.
Diethylstilbestrol is first oral activated synthetic estrogen.But because it has the promotion growth of animal, increase proteins deposited and effect that improve food conversion ratio, also be used as growth accelerator, be widely used in livestock breeding industry.The residual meeting of diethylstilbestrol gets muddled normal person's physiological function, more seriously influences children and grows normally.Recent research finds that diethylstilbestrol can cause animal and human's cancer and teratogenesis toxicity, and the pregnancy duration use also can cause offspring's genital system diseases.Therefore, world many countries forbids using in feed diethylstilbestrol to promote growth of animal.
This three parahormone can promote maiden's precocity greatly, therefore, is necessary to especially animal products being carried out these a few class estrogen detections in the food.
At present, prior art mainly relies on chemical apparatuses analysis, radio-immunity detection kit and enzyme immunoassay kit for the detection of this three classes estrogens feed addictive, these method operating process are loaded down with trivial details, the time is long, operating personnel must have certain specialized technical knowledge and could operate, so range of application is limited.The test strip that independent detection estradiol, estriol and diethylstilbestrol are also arranged, but up to the present, do not detect the appliance of these three kinds of materials simultaneously.
Summary of the invention
The purpose of this utility model be to provide a kind of simple and efficient to handle, cost is low, easy to carry, the layman also can operate and can carry out the on-the-spot colloid gold test paper that detects, and can detect three class female hormone simultaneously, overcome prior art complex operation, specialty and require high and the defective that detects of individual event one by one.
The technical solution of the utility model: a kind of estradiol, estriol and diethylstilbestrol three test card: it is provided with test-strips 2 in the test card shell 1 of rectangular flat shell shape, the test card case surface has the fenestra 3 of detection and well 4, and test-strips 2 is by pasting sample pad 8 thereon successively by adhesive sticker on the supporting backboard 5, the glass fibre membrane 11 that contains anti-estradiol antibody colloidal gold label, the glass fibre membrane 10 that contains anti-estriol antibody colloidal gold label, the glass fibre membrane 7 that contains anti-diethylstilbestrol antibody colloid gold label thing, nitrocellulose filter 6 and absorbing membrane 9 are formed; The stacked stickup nitrocellulose filter 6 in supporting backboard middle part, one endlap of supporting backboard is put and is pasted absorbing membrane 9, the stacked stickup sample pad 8 of the other end, absorbing membrane is inner to overlap with nitrocellulose filter respectively separately with sample pad the inner, at the clinch of sample pad and nitrocellulose filter, insert and put between the two and paste three sections collaurum films 11,10,7; Collaurum film 11,10,7 is the glass fibre membrane that contains anti-estradiol antibody colloidal gold label successively, the glass fibre membrane of anti-estriol antibody colloidal gold label, and the glass fibre membrane of anti-diethylstilbestrol antibody colloid gold label thing, there are three to detect band and a quality control band on the nitrocellulose filter, be successively: contain the detection of estradiol protein conjugate and be with 12, contain the detection of estriol protein conjugate and be with 13, contain the detection of diethylstilbestrol protein conjugate and be with 14, quality control band 15, quality control band is anti-rabbit antibody or anti-mouse antibody, test-strips is inserted in the test card shell, sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
The disposal route of described estradiol, estriol and diethylstilbestrol three test card test sample, test sample is urine sample, feed sample or animal tissue, meat, liver sample, for various test sample different disposal routes is arranged;
The disposal route of urine sample: urine directly as test sample, get limpid supernatant and make the specimen sample by the urine filter paper filtering of muddy state, and the urine of clarification is directly made specimen; Specimen is used for detecting after splashing into well.
The disposal route of feed sample: take by weighing 0.5 gram feed sample, add 1mL sample extract, in test tube, stirred 1 minute, leave standstill 10 minutes after, get and be used for detecting after limpid supernatant splashes into well as specimen.
The disposal route of animal tissue, meat, liver sample: get that 1g shreds or the animal tissue of homogenate, meat, liver sample, add and contain in the test tube of 1mL sample extract, tighten the pipe lid, behind the jolting sample 1 minute, through 100 ℃ of water-bath heating 5 minutes, be cooled to room temperature, get limpid center section and splash into as specimen and be used for behind the well detecting.
Described sample extract is pH 7.0, concentration 50mmol/L Tris-HCL damping fluid.
The beneficial effects of the utility model: three test card of the present invention can detect estradiol, estriol and the diethylstilbestrol that contains in urine or feed, animal tissue, meat, the liver simultaneously, these three test card are easy to preparation and save testing cost, easy to use quick, the result is accurate.
Description of drawings
The shell of Fig. 1 estradiol, estriol and diethylstilbestrol three test card and test-strips arrangement figure in the enclosure.1, test card shell, 2, test-strips, 3, detect fenestra, 4, well.
The test-strips structural representation of Fig. 2 estradiol, estriol and diethylstilbestrol three test card.5, supporting backboard, 6, nitrocellulose filter, 7, contain the collaurum film of anti-diethylstilbestrol antibody colloid gold label thing, 8, sample pad, 9, absorbing membrane, 10, contain the collaurum film of anti-estriol antibody colloidal gold label, 11, contain the collaurum film of anti-estradiol antibody colloidal gold label.
Show the trace synoptic diagram on the nitrocellulose filter of Fig. 3 estradiol, estriol and diethylstilbestrol three test card.6, nitrocellulose filter, 12, contain the estradiol protein conjugate and detect band, 13, contain the estriol protein conjugate and detect band, 14, contain the diethylstilbestrol protein conjugate and detect band, 15, quality control band.
Embodiment
The utility model further specifies as follows in conjunction with specific embodiments referring to accompanying drawing.
A kind of estradiol, estriol and diethylstilbestrol three test card, it is that test-strips 2 is set in test card shell 1, the setting of the structure of test card shell 1 and test-strips 2 is referring to accompanying drawing 1, Fig. 2.Test card shell 1 is rectangular flat shell shape shell, long 7cm, and wide 2cm, thick 0.5cm, shell wall thickness 0.1cm is made by engineering plastics.Test card shell 1 is connected with the shell block two halves by cap and constitutes, and the fenestra 3 of detection and well 4 are arranged on test card shell 1 cap.Test-strips 2 is placed in the shell block of test card shell 1.
Test-strips 2 is fillet thin slices of sandwich construction, and sheet is about 6.0cm, the wide about 0.4cm of sheet, and thickness is less than 2.5mm.Test-strips 2 is sandwich constructions: bottom is a supporting backboard 5, is the igelite thin slice, thick about 0.5mm, long 6.0cm, wide 0.4cm; The stacked stickup nitrocellulose filter 6 in supporting backboard 5 middle parts, cellulose nitrate thickness 0.5mm, long 2-2.5cm, wide 0.4cm.Four isolated horizontal demonstration trace bands are arranged on the nitrocellulose filter 6, referring to accompanying drawing 3, article four, show in the trace that article one is to detect to be with 12, contains the estradiol protein conjugate, second detects and is with 13, contain the estriol protein conjugate, the 3rd is to detect to be with 14, contains the diethylstilbestrol protein conjugate, article four, be quality control band 15, contain anti-rabbit antibody or anti-mouse antibody.Regulate protein conjugate concentration and be approximately 1mg/ml, spray film when anti-rabbit antibody or anti-mouse antibody concentration are approximately 0.1mg/ml.Stacked stickup absorbing membrane 9 on supporting backboard 5 one ends, stacked stickup sample pad 8 on supporting backboard 5 other ends, absorbing membrane 9 the inners overlap with nitrocellulose filter 6 one ends respectively separately with sample pad 8 the inners, at the clinch of sample pad 8 with nitrocellulose filter 6, insert and put between the two and paste three sections collaurum films 11,10,7, collaurum film 11,10,7 is glass fibre membrane, and the glass fibre membrane of anti-diethylstilbestrol antibody colloid gold label thing of the glass fibre membrane that contains anti-estradiol antibody colloidal gold label, anti-estriol antibody colloidal gold label successively.Collaurum thickness 0.5-0.8mm, long 0.5-0.6cm, wide 0.4cm.Sample pad 8 thick 0.5-0.8mm, long 2cm, wide 0.4cm.Absorbing membrane 9 thick 0.5-0.8mm, long 1.6-2cm, wide 0.4cm.The collaurum film is with behind antibody and the colloid gold particle mark, regulates concentration and is approximately 1D coating colloid glass fibre membrane, and the oven dry back is standby.Collaurum film 11,107, sample pad 8 and absorbing membrane 9 are 0.1-0.2cm with nitrocellulose filter 6 laps of splice.Absorbing membrane 9 materials are thieving paper, and sample pad 8 materials are water adsorption glass tunica fibrosas.
The utility model estradiol, estriol and diethylstilbestrol three test card are used for detecting livestock feed or animal urine sample or animal tissue, meat, whether liver contains estradiol, estriol and diethylstilbestrol, differentiate the security of herding food in view of the above.
The disposal route of test sample:
Disposal route at urine sample is: urine, needs to filter through filter paper earlier if urine sample is muddy shape directly as test sample, gets supernatant and makes specimen; If specimen is then directly made in the urine clarification.
Disposal route at the feed sample is: take by weighing 0.5 gram feed sample, add 1mL sample extract (Tris-HCL of pH 7.0,50mmol/L), in test tube, stirred 1 minute, leave standstill 10 minutes after, get limpid supernatant and make specimen.
Disposal route at animal tissue, meat, liver sample is: get 1 gram and shred or animal tissue, meat, the liver sample of homogenate, place the test tube that contains 1mL sample extract (Tris-HCL of pH 7.0,50mmol/L), tighten the pipe lid, behind the jolting sample one minute, through 100 ℃ of water-baths 5 minutes, be cooled to room temperature, get limpid middle clear liquid and make specimen.
Use estradiol, estriol and diethylstilbestrol three joint inspections to survey and stick into when going detection, earlier sample liquid to be detected is instilled into the sample pad 8 of estradiol, estriol and diethylstilbestrol three test card from well 4, because syphonic effect principle, drive the contained anti-estradiol of sample liquid to be detected and collaurum film, anti-estriol and anti-diethylstilbestrol monoclonal antibody or polyclonal antibody colloid gold label thing together to nitrocellulose filter 6 diffusions, observations in 5-10 minute.
The key reaction of test card is immunologic antigen and antibody response, the antibody of the colloid gold label that on nitrocellulose filter, moves, on calibration tape respectively with contain the estradiol protein conjugate, contain the estriol protein conjugate, contain the diethylstilbestrol protein conjugate, and anti-rabbit antibody of quality control band or anti-mouse antibody reaction, form the brownish red band.In sample, there are corresponding estradiol to be checked, estriol and diethylstilbestrol to exist, then sample promptly reacts with corresponding antibody after adding, and can be with the contained estradiol protein conjugate of relevant detection band, contain the estriol protein conjugate, contain the reaction of diethylstilbestrol protein conjugate, thereby do not develop the color.The result of key reaction has following 9 kinds of situations:
1, described detection be with 12,13,14 and quality control band 15 manifest brownish red trace band simultaneously, the expression testing result is negative, illustrates that estradiol in the detected sample, estriol and diethylstilbestrol content all surpass permissible value.
2,12,13,14 Show Colors not are with in described detection, have only quality control band 15 to manifest the brownish red trace, and testing result is positive, shows that estradiol in the detected sample, estriol and diethylstilbestrol content are all above permissible value.
3,12,13 Show Colors not are with in described detection, detect be with 14 and quality control band 15 manifest the brownish red trace simultaneously, testing result is positive, show in the detected sample estradiol and estriol content all above permissible value, and diethylstilbestrol content is above permissible value.
4,12,14 Show Colors not are with in described detection, detect be with 13 and quality control band 15 manifest the brownish red trace simultaneously, testing result is positive, show in the detected sample estradiol and diethylstilbestrol content all above permissible value, and estriol content is above permissible value.
5,13,14 Show Colors not are with in described detection, detect be with 12 and quality control band 15 manifest the brownish red trace simultaneously, testing result is positive, show in the detected sample estriol and diethylstilbestrol content all above permissible value, and estradiol content is above permissible value.
6,12 Show Colors not are with in described detection, detect be with 13,14 and quality control band 15 manifest the brownish red trace simultaneously, testing result is positive, shows that estradiol content surpasses permissible value in the detected sample, and estriol, diethylstilbestrol content all surpass permissible value.
7,13 Show Colors not are with in described detection, detect be with 12,14 and quality control band 15 manifest the brownish red trace simultaneously, testing result is positive, shows that estriol content surpasses permissible value in the detected sample, and estradiol, diethylstilbestrol content all surpass permissible value.
8,14 Show Colors not are with in described detection, detect be with 12,13 and quality control band 15 manifest the brownish red trace simultaneously, testing result is positive, shows that diethylstilbestrol content surpasses permissible value in the detected sample, and estradiol, estriol content all surpass permissible value.
9, described detection be with 12,13,14 and quality control band 15 all do not develop the color, show that then test-strips lost efficacy.
Claims (1)
1. estradiol, estriol and diethylstilbestrol three test card, it is characterized in that: it is provided with test-strips (2) in the test card shell (1) of rectangular flat shell shape, the test card case surface has detection fenestra (3) and well (4), and test-strips (2) is to be gone up by supporting backboard (5) to paste sample pad (8) thereon successively by adhesive sticker, the glass fibre membrane (11) that contains anti-estradiol antibody colloidal gold label, the glass fibre membrane (10) that contains anti-estriol antibody colloidal gold label, the glass fibre membrane (7) that contains anti-diethylstilbestrol antibody colloid gold label thing, nitrocellulose filter (6), and absorbing membrane (9) is formed; Supporting backboard middle part stacked stickup nitrocellulose filter (6), one endlap of supporting backboard is put and is pasted absorbing membrane (9), the stacked stickup sample pad of the other end (8), absorbing membrane is inner to overlap with nitrocellulose filter respectively separately with sample pad the inner, clinch at sample pad and nitrocellulose filter, insert and put the glass fibre membrane (11) of pasting anti-estradiol antibody colloidal gold label between the two, the glass fibre membrane (10) of anti-estriol antibody colloidal gold label, and the glass fibre membrane (7) of anti-diethylstilbestrol antibody colloid gold label thing, there are three to detect band and a quality control band on the nitrocellulose filter, be successively: contain the estradiol protein conjugate and detect band (12), contain the estriol protein conjugate and detect band (13), contain the diethylstilbestrol protein conjugate and detect band (14), quality control band (15), quality control band is anti-rabbit antibody or anti-mouse antibody, test-strips is inserted in the test card shell, sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206129441U CN201876459U (en) | 2010-11-18 | 2010-11-18 | Trilink detecting card of estradiol, trihydroxyoestrin and diethylstilbestrol |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010206129441U CN201876459U (en) | 2010-11-18 | 2010-11-18 | Trilink detecting card of estradiol, trihydroxyoestrin and diethylstilbestrol |
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| Publication Number | Publication Date |
|---|---|
| CN201876459U true CN201876459U (en) | 2011-06-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010206129441U Expired - Fee Related CN201876459U (en) | 2010-11-18 | 2010-11-18 | Trilink detecting card of estradiol, trihydroxyoestrin and diethylstilbestrol |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112326976A (en) * | 2020-11-04 | 2021-02-05 | 瑞莱生物科技江苏有限公司 | Fluorescence quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotropin |
-
2010
- 2010-11-18 CN CN2010206129441U patent/CN201876459U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112326976A (en) * | 2020-11-04 | 2021-02-05 | 瑞莱生物科技江苏有限公司 | Fluorescence quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotropin |
| CN112326976B (en) * | 2020-11-04 | 2024-04-26 | 瑞莱生物科技江苏有限公司 | Fluorescent quantitative detection kit for progesterone, estradiol and beta-human chorionic gonadotrophin |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110622 Termination date: 20171118 |
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| CF01 | Termination of patent right due to non-payment of annual fee |