CN202013356U - Colloidal gold detection kit for dolantin - Google Patents
Colloidal gold detection kit for dolantin Download PDFInfo
- Publication number
- CN202013356U CN202013356U CN2011201961277U CN201120196127U CN202013356U CN 202013356 U CN202013356 U CN 202013356U CN 2011201961277 U CN2011201961277 U CN 2011201961277U CN 201120196127 U CN201120196127 U CN 201120196127U CN 202013356 U CN202013356 U CN 202013356U
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- China
- Prior art keywords
- sauteralgyl
- paper
- kit
- reagent
- cellulose nitrate
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Abstract
The utility model discloses a colloidal gold detection kit for dolantin. The colloidal gold detection kit comprises a kit cover, a kit seat and reagent strips, wherein the reagent strips are fixed on the kit seat, and the kit cover is clamped on the kit seat; a circle sample injecting hole is arranged at one end of the kit cover, and an oval observation opening is arranged at the middle part of the kit cover; each reagent strip comprises a rubber plate paper layer, a filtering sample paper, a glass fiber gasket, a nitrocellulose diaphragm and an absorbent paper, wherein the filtering sample paper, the glass fiber gasket, the nitrocellulose diaphragm and the absorbent paper are sequentially arranged on the rubber plate paper layer end to end; the filtering sample paper is positioned correspondingly to the circle sample injecting hole, and the nitrocellulose diaphragm is positioned correspondingly to the oval observation opening; a monoclonal antibody colloidal gold layer resisting the dolantin is wrapped on the glass fiber gasket; and detection lines and quality control lines are parallelly arranged on the nitrocellulose diaphragm at intervals, a dolantin carrier complexes layer is wrapped on the detection lines, and an antibody layer of colloidal gold marked goat-antimouse IgG (Immunoglobulin G) is wrapped on the quality control lines. The colloidal gold detection kit has high sensibility, strong specificity, is simple to operate, is time-saving and is very applicable to be used on site.
Description
Technical field
The utility model relates to immune colloid gold detection kit technical field, relates in particular to a kind of gold-immunochromatographyreagent reagent for assay box that is used to detect Sauteralgyl.
Background technology
The formal name used at school of Sauteralgyl is called mark pyridine, demerol again pethidine, is the morphine substitute of synthetic, and its hydrochloride is the white crystals sprills, and nothing is smelt, and the water-soluble and ethanol of energy is made generally in injection.The mechanism of action to human body is similar to morphine, but glad, analgesic activity is little than morphine, has certain habituation, be put into national narcotics and give control.But Sauteralgyl uses habituation continuously, uses 1-2 week just can produce dependence continuously.In case after stopping then can producing withdrawal syndrome behind the morphine abstinence syndrome.The abuse of Sauteralgyl is one of current drug issue that faces of China.
At present, the utilization of the detection method of Sauteralgyl mainly is the method that gas chromatography GC, high-efficient liquid phase chromatogram HPLC, gaseous mass spectrum GC/MS or GC/FID analyze.The main deficiency of above prior art is that the detection method complexity is time-consuming, and the cost height is not suitable for on-the-spot instant detection the to Sauteralgyl.Therefore, domestic drug law enforcement, look into and press for a kind of easy to detect, detection kit fast in poison, the counterdrug operation.
The utility model content
Technical problem to be solved in the utility model is exactly in order to overcome the deficiencies in the prior art, a kind of detection kit that is used to detect Sauteralgyl of utilizing the colloidal gold immunochromatographimethod method preparation is provided, and this detection kit possesses sensitivity, special, easy, quick, the suitable on-the-spot instant characteristics that detect.
Technical problem to be solved in the utility model can realize by following technical proposals:
A kind of gold-immunochromatographyreagent reagent for assay box that is used to detect Sauteralgyl comprises lid, cassette holder and reagent strip, and wherein said reagent strip is fixed on the cassette holder, and described lid is fastened on the cassette holder; End at described lid offers circular well, and the centre of lid offers oval observation panel; Described reagent strip comprises the offset plate ply of paper and from the beginning to the end is successively set on filter sample paper, glass fibre pad, cellulose nitrate diaphragm and thieving paper on the described offset plate ply of paper; The position of described filter sample paper is corresponding to described circular well, and the position of described cellulose nitrate diaphragm is corresponding to described oval observation panel; On described glass fibre pad, be coated with anti-Sauteralgyl monoclonal antibody collaurum layer; On described cellulose nitrate diaphragm, be arranged at intervals with parallel detection line and nature controlling line, wherein said detection line is arranged on the end that described cellulose nitrate diaphragm closes on described circular well, and nature controlling line is arranged on the end of described cellulose nitrate diaphragm away from described circular well; Described detection line is coated with Sauteralgyl carrier complexes layer, and described nature controlling line is coated with gold mark sheep anti-mouse igg antibody layer.
In a preferred embodiment of the present utility model, the width of described detection line and nature controlling line is 0.1～1.5mm.
In a preferred embodiment of the present utility model, described circular well is up big and down small funnel shaped, can prevent effectively that sample from overflowing.
Described cassette holder is provided with the reagent strip mounting groove, and described reagent strip is placed in the described reagent strip mounting groove.
In a preferred embodiment of the present utility model, one end of described filter sample paper is folded mutually with an end of described glass fibre pad, the other end of described glass fibre pad is folded mutually with an end of cellulose nitrate diaphragm, and the other end of described cellulose nitrate diaphragm is folded mutually with thieving paper.
During use, in circular well, drip 3 (about 120 μ l) urine samples, 3-8 minute sentence read result after dripping sample.If contain Sauteralgyl in the urine sample, Sauteralgyl in the urine sample will with the Sauteralgyl monoclonal antibody reactive in the anti-Sauteralgyl monoclonal antibody collaurum layer that is coated on the cellulose nitrate diaphragm, competition is marked at the antigen binding site on the anti-Sauteralgyl monoclonal antibody collaurum layer, when the Sauteralgyl concentration in the urine is higher than 1000ng/ml, Sauteralgyl in the urine sample can be at first with the Sauteralgyl monoclonal antibody reactive and occupy whole antigen binding sites, so just stoped the Sauteralgyl carrier in the Sauteralgyl carrier complexes layer on Sauteralgyl monoclonal antibody and the detection line in the anti-Sauteralgyl monoclonal antibody collaurum layer to combine, detection line can not capture colloid gold particle and not have red ribbon to present, positive result.If do not have Sauteralgyl or its concentration to be lower than threshold concentration in the urine sample, Sauteralgyl monoclonal antibody in the then anti-Sauteralgyl monoclonal antibody collaurum layer will combine with the Sauteralgyl carrier in the Sauteralgyl carrier complexes layer on the detection line, detection line captures colloid gold particle and presents red ribbon, negative result.
Negative: as two colour bands in oval observation panel, to occur.Be that red lines respectively appear in detection line (T line) and nature controlling line (C line) position.No Sauteralgyl exists in the expression sample.
Positive: only red lines appear in nature controlling line (C line) position in oval observation panel, and any lines do not appear in detection line (T line), have Sauteralgyl to exist in the expression sample.
Invalid: because under any circumstance, red lines appear in nature controlling line (C line) position, and expression application of sample and operation are correct.If red lines do not appear in nature controlling line (C line) position, show that test result is uncertain, should reform.
Gold-immunochromatographyreagent reagent for assay box susceptibility height, high specificity, easy and simple to handle the saving time that is used to detect Sauteralgyl of the present utility model, very suitable on-the-spot the use.
Description of drawings
Fig. 1 is the gold-immunochromatographyreagent reagent for assay box front schematic view that is used to detect Sauteralgyl of the present utility model.
Fig. 2 removes inner structure synoptic diagram behind the lid for the gold-immunochromatographyreagent reagent for assay box that is used to detect Sauteralgyl of the present utility model.
Fig. 3 is the reagent strip structural representation that is used to detect the gold-immunochromatographyreagent reagent for assay box of Sauteralgyl of the present utility model.
Embodiment
Describe the utility model in detail below in conjunction with accompanying drawing.
Referring to Fig. 1 to Fig. 3, being used to shown in the figure detected the gold-immunochromatographyreagent reagent for assay box of Sauteralgyl, comprises lid 2, cassette holder 1 and reagent strip 3, in corresponding respectively being provided with draw-in groove and blocking prominent in the edge of lid 2 and cassette holder 1, the prominent engaging mutually of draw-in groove and card is buckled on the cassette holder 1 lid 2.End at lid 2 offers a circular well 4, and circular well 4 is up big and down small funnel shaped, can prevent effectively that sample from overflowing.Offer oval observation panel 5 in the centre of lid 2, oval observation panel 5 also is up big and down small funnel shaped.Cassette holder 1 is provided with the reagent strip mounting groove, and reagent strip 3 is placed in this reagent strip mounting groove.
On glass fibre pad 7, be coated with anti-Sauteralgyl monoclonal antibody collaurum layer, on cellulose nitrate diaphragm 8, be arranged at intervals with parallel detection line 10 and nature controlling line 11, detection line 10 is arranged on the end that cellulose nitrate diaphragm 8 closes on circular well 4, and nature controlling line 11 is arranged on the end of cellulose nitrate diaphragm 8 away from circular well 4; The width of detection line 10 and nature controlling line 11 is 0.7mm, detection line 10 is coated with Sauteralgyl carrier complexes layer, nature controlling line 11 is coated with gold mark sheep anti-mouse igg antibody layer, and Sauteralgyl carrier complexes layer and gold mark sheep anti-mouse igg antibody layer all adopt the specking mode to be fixed on above the reagent strip.Oval observation panel 5 lateral margins at lid 2 are provided with mark " C " and " T ", and mark " C " is corresponding to nature controlling line 11, and mark " T " is corresponding to detection line 10.
Claims (5)
1. be used to detect the gold-immunochromatographyreagent reagent for assay box of Sauteralgyl, comprise lid, cassette holder and reagent strip, wherein said reagent strip is fixed on the cassette holder, and described lid is fastened on the cassette holder; It is characterized in that offer circular well at an end of described lid, the centre of lid offers oval observation panel; Described reagent strip comprises the offset plate ply of paper and from the beginning to the end is successively set on filter sample paper, glass fibre pad, cellulose nitrate diaphragm and thieving paper on the described offset plate ply of paper; The position of described filter sample paper is corresponding to described circular well, and the position of described cellulose nitrate diaphragm is corresponding to described oval observation panel; On described glass fibre pad, be coated with anti-Sauteralgyl monoclonal antibody collaurum layer; On described cellulose nitrate diaphragm, be arranged at intervals with parallel detection line and nature controlling line, wherein said detection line is arranged on the end that described cellulose nitrate diaphragm closes on described circular well, and nature controlling line is arranged on the end of described cellulose nitrate diaphragm away from described circular well; Described detection line is coated with Sauteralgyl carrier complexes layer, and described nature controlling line is coated with gold mark sheep anti-mouse igg antibody layer.
2. the gold-immunochromatographyreagent reagent for assay box that is used to detect Sauteralgyl as claimed in claim 1 is characterized in that the width of described detection line and nature controlling line is 0.1～1.5mm.
3. the gold-immunochromatographyreagent reagent for assay box that is used to detect Sauteralgyl as claimed in claim 1 is characterized in that described circular well is up big and down small funnel shaped.
4. the gold-immunochromatographyreagent reagent for assay box that is used to detect Sauteralgyl as claimed in claim 1 is characterized in that described cassette holder is provided with the reagent strip mounting groove, and described reagent strip is placed in the described reagent strip mounting groove.
5. the gold-immunochromatographyreagent reagent for assay box that is used to detect Sauteralgyl as claimed in claim 1, it is characterized in that, one end of described filter sample paper is folded mutually with an end of described glass fibre pad, the other end of described glass fibre pad is folded mutually with an end of cellulose nitrate diaphragm, and the other end of described cellulose nitrate diaphragm is folded mutually with thieving paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201961277U CN202013356U (en) | 2011-06-12 | 2011-06-12 | Colloidal gold detection kit for dolantin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201961277U CN202013356U (en) | 2011-06-12 | 2011-06-12 | Colloidal gold detection kit for dolantin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202013356U true CN202013356U (en) | 2011-10-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011201961277U Expired - Lifetime CN202013356U (en) | 2011-06-12 | 2011-06-12 | Colloidal gold detection kit for dolantin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202013356U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102520181A (en) * | 2011-12-31 | 2012-06-27 | 上海凯创生物技术有限公司 | Colloidal gold assay kit for rapidly detecting dolantin and preparation of kit |
| CN108614105A (en) * | 2018-04-23 | 2018-10-02 | 苏州仁端生物医药科技有限公司 | A kind of reagent strip and its application |
| CN108828238A (en) * | 2018-08-30 | 2018-11-16 | 苏州仁端生物医药科技有限公司 | A kind of A β1-42Detection reagent item and its application |
| CN109018612A (en) * | 2018-07-05 | 2018-12-18 | 嘉兴凯实生物科技有限公司 | A kind of reagent strip and kit |
-
2011
- 2011-06-12 CN CN2011201961277U patent/CN202013356U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102520181A (en) * | 2011-12-31 | 2012-06-27 | 上海凯创生物技术有限公司 | Colloidal gold assay kit for rapidly detecting dolantin and preparation of kit |
| CN102520181B (en) * | 2011-12-31 | 2014-07-16 | 上海凯创生物技术有限公司 | Colloidal gold assay kit for rapidly detecting dolantin and preparation of kit |
| CN108614105A (en) * | 2018-04-23 | 2018-10-02 | 苏州仁端生物医药科技有限公司 | A kind of reagent strip and its application |
| CN109018612A (en) * | 2018-07-05 | 2018-12-18 | 嘉兴凯实生物科技有限公司 | A kind of reagent strip and kit |
| CN108828238A (en) * | 2018-08-30 | 2018-11-16 | 苏州仁端生物医药科技有限公司 | A kind of A β1-42Detection reagent item and its application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20111019 |