CN202735346U - Muti-index joint inspection reagent card - Google Patents
Muti-index joint inspection reagent card Download PDFInfo
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- CN202735346U CN202735346U CN 201220407441 CN201220407441U CN202735346U CN 202735346 U CN202735346 U CN 202735346U CN 201220407441 CN201220407441 CN 201220407441 CN 201220407441 U CN201220407441 U CN 201220407441U CN 202735346 U CN202735346 U CN 202735346U
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- test strips
- base plate
- reagent card
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Abstract
The utility model discloses a muti-index joint inspection reagent card which comprises a base plate, a top cover matching with the base plate, and a card cap, wherein the base plate is provided at least two mutually-separated fixed grooves each of which is provided with a test scrip, the top cover is provided with a result indication window and a detection project indication window respectively at positions corresponding to each test scrip, and one end of the test scrip is protruded and inserted into the card cap. The muti-index joint inspection reagent card can realize joint inspection of multiple projects for one time, and analyze multiple results, thereby saving labor and time. According to the utility model, the fixed grooves are mutually separated, and thus the test scrips are mutually separated, so that each detection project does not have cross contamination, and does not affects each other due to chromatography effect which may lead to deviation of test results.
Description
Technical field
The utility model relates to a kind of combination assay reagent card.
Background technology
After " the melamine event " of Sanlu arranged first two " clenbuterol hydrochloride events " of converging are arranged, nowadays food-safety problem has become the problem that must pay attention in our life.For " clenbuterol hydrochloride " class medicine commonly used, present stage has had can detect clenobuterol hydrochloride, Ractopamine, salbutamol isocolloid gold test card, has realized the fast detecting of " clenbuterol hydrochloride ".
Clenobuterol hydrochloride, Ractopamine, salbutamol collaurum rapid detection card are the test card for detection of clenobuterol hydrochloride, Ractopamine, the single project of salbutamol, can detect whether to contain this type of material in the sample fast qualitative.Solidify corresponding clenobuterol hydrochloride, Ractopamine, salbutamol antigen at the NC film, the colloid gold label specific antibody adopts competition to suppress principle and carries out the fast qualitative detection.
But present detection kit still has the following disadvantages: at first, common most of detection kit all are the detections of single index, and the analysis data that obtain are single, and are not comprehensive, helpless to the detection of multinomial composite index.Secondly, the reagent box for multi-index joint inspection that has gone on the market still adopts application of sample pore structure of a test strips, will carry out application of sample, troublesome poeration to each well during detection.The 3rd, at the kit of same test strips detection 3 " clenbuterol hydrochlorides ", show respectively the reaction result of sample diverse location after the test strips diffusion, this type of reagent box for multi-index joint inspection, the index that can detect is limited, produces easily the phase mutual interference between the reaction result, affects interpretation of result.
The utility model content
Based on this, the utility model provides a kind of combination assay reagent card that can carry out once the qualitative detection of a plurality of projects in same test card.
A kind of combination assay reagent card, comprise base plate, with the supporting loam cake of base plate, and card cap, offer at least two pickup grooves of mutually separating on the described base plate, be provided with a test strips in each pickup groove, as a result indicating window and test item indicating window are offered respectively in the position that described loam cake is corresponding with every test strips; One distal process of described test strips goes out, and inserts in the described card cap.
Among embodiment, the pickup groove of offering on the described base plate is three therein.Be provided with a test strips in each pickup groove, be respectively the test strips that detects clenobuterol hydrochloride, Ractopamine and salbutamol.
Among embodiment, described test strips is the colloidal gold immunochromatographydetection detection test paper bar, is provided with detection zone and control zone on it therein, the corresponding described detection zone of described as a result indicating window and control zone.
Among embodiment, be provided with the test item indicator on the described test strips therein, the corresponding described test item of described test item indicating window indicator.
The utility model utilizes colloidal gold immuno-chromatography test paper strip to realize the joint inspection of a plurality of projects, colloidal gold immuno-chromatography test paper strip is to adopt competitive immunization chromatography ratio juris, with collaurum as the trace labelling thing, clenobuterol hydrochloride, Ractopamine, salbutamolum residue in the fast detecting animals urine.Be coated with clenobuterol hydrochloride-BSA, Ractopamine-BSA, salbutamol-BSA conjugate and goat anti-rabbit igg antibody on the corresponding test strips nitrocellulose filter, in sample, contain clenobuterol hydrochloride concentration 〉=3ng/ml, Ractopamine concentration 〉=3ng/ml, salbutamol concentration 〉=5ng/ml, corresponding detection line (T line) does not develop the color, and this moment, the result was this positive; And in the sample clenobuterol hydrochloride concentration≤3ng/ml, Ractopamine concentration≤3ng/ml, salbutamol concentration≤5ng/ml or do not contain sometimes, detection line (T line) will develop the color, the result is negative at this moment.No matter whether contain clenobuterol hydrochloride, Ractopamine, salbutamol in the sample, nature controlling line (C line) all will develop the color, and will be effective to show product.
The utility model can once can detect in the sample whether contain clenobuterol hydrochloride, Ractopamine, salbutamol by fast qualitative, realizes the entry joint inspection, and multinomial interpretation of result is time saving and energy saving.
Space between the pickup groove of the present utility model makes between test strips also space, and each test item cross pollution can not occur, and affects testing result.
The utility model both can adopt the dropping mode to add sample or it had directly been detected in the insertion sample, and dirigibility is large, more convenient operation.
Description of drawings
Fig. 1 is the structural representation of the utility model combination assay reagent card;
Fig. 2 is the synoptic diagram of opening of the utility model combination assay reagent card;
Fig. 3 is the structural representation of the interior test strips of the combination assay reagent card of Fig. 1;
Reference numeral: 1, base plate; 2, loam cake; 3, card cap; 4, pickup groove; 5, colloidal gold immunochromatographydetection detection test paper bar; 6, indicating window as a result; 7, test item indicating window; 51-test strips end liner; The 52-sample pad; 53-colloid gold particle label pad; The 54-nitrocellulose filter; 55-thieving paper.
Embodiment
Describe the present invention in detail below in conjunction with the drawings and specific embodiments.
Embodiment 1 three index joint inspection reagent cards
See also Fig. 1-3, be a kind of many index joint inspection reagent card of the present invention, comprise base plate 1, with the supporting loam cake 2 of base plate 1, and card cap 3, offer three pickup grooves 4 of mutually separating on the described base plate 1, be provided with a colloidal gold immunochromatographydetection detection test paper bar 5 in each pickup groove 4, described three test strips 5 are respectively the test strips that detects clenobuterol hydrochloride, Ractopamine and salbutamol, be added with respectively the test item indicating label on the described test strips 5, as the test item indicator; As a result indicating window 6 and test item indicating window 7 are offered respectively in described loam cake 2 and every test strips 5 corresponding positions, detection zone and control zone on the described as a result indicating window 6 corresponding test strips 5, the test item indicator on the described test item indicating window 7 corresponding test strips; One distal process of described test strips 5 goes out, and inserts in the described card cap 3.
Described test strips 5 is overlapped to stick on the end liner 51 in turn by sample pad 52, colloid gold particle label pad 53, nitrocellulose filter 54, thieving paper 55 and consists of, what be coated with respectively on the colloid gold particle label pad 53 of described every test strips 5 is antibody of clenbuteral label, Anti-ractopamine antibody label, salbutamol antibody labeling thing, coated concentration is 5-10%, and coated coefficient is 25ml/30cm
2Nitrocellulose filter 54 is coated with respectively clenobuterol hydrochloride-BSA, Ractopamine-BSA, salbutamol-BSA conjugate (coated concentration is that 0.1-0.3mg/ml, coated coefficient are 100ug/cm), form detection zone, and goat anti-rabbit igg antibody (coated concentration is that 0.5mg/ml and coated coefficient are 100ug/cm), the formation control district.
Utilize the reagent card of this embodiment at room temperature to detect, detecting step is:
(1) aluminium foil bag that will pack reagent card is torn along cutting part, takes out reagent card and is put on the smooth table top, and carry out mark;
(2) take off the card cap, the end (reconstitute end) that test strips is outstanding inserts in the urine, and the urine liquid level surpasses " MAX " line, climbs to take out to the view window as a result until liquid to keep flat; Or take off the card cap, and draw urine specimen with suction pipe, vertically add 3 (about 60 μ l) in the end (reconstitute end) that test strips is given prominence to, should not bring bubble in the sample;
Observe the result who shows in (3) 5 minutes, the result who shows after 10 minutes is meaningless.
If indicating window just shows a red line as a result, namely only there is nature controlling line (C line) to become redness, detection line (T line) does not develop the color, show clenobuterol hydrochloride in the sample Ractopamine the content of salbutamol be greater than or equal to detection sensitivity 3ng/ml 3ng/ml 5ng/ml, the result is positive; If what indicating window 6 showed as a result is two red line, be that detection line (T line) and nature controlling line (C line) all become redness, show not hydrochloric Clenbuterol in the sample Ractopamine salbutamol or clenobuterol hydrochloride Ractopamine the content of salbutamol be lower than each detection sensitivity, the result is negative; If what indicating window 6 showed as a result is that nature controlling line (C line) does not develop the color, it is invalid to detect, and the result is invalid, and suggestion retests with neocaine.
Embodiment 2 two indexes joint inspection reagent cards
The reagent card structure of present embodiment is identical with the structure of embodiment 1 reagent card, and different is, pickup groove is two, and the test strips that arranges in the pickup groove is respectively the test strips that detects clenobuterol hydrochloride and salbutamol.
Operate according to embodiment 1 identical method of operating, utilize as can be known the test strips of present embodiment, can realize that the associating of clenobuterol hydrochloride and salbutamol quantitatively detects.
Embodiment 3 two indexes joint inspection reagent cards
The reagent card structure of present embodiment is identical with the structure of embodiment 1 reagent card, and different is, pickup groove is two, and the test strips that arranges in the pickup groove is respectively the test strips that detects clenobuterol hydrochloride and Ractopamine.
Operate according to embodiment 1 identical method of operating, utilize as can be known the test strips of present embodiment, can realize that the associating of clenobuterol hydrochloride and Ractopamine quantitatively detects.
Embodiment 4 two indexes joint inspection reagent cards
The reagent card structure of present embodiment is identical with the structure of embodiment 1 reagent card, and different is, pickup groove is two, and the test strips that arranges in the pickup groove is respectively the test strips that detects salbutamol and Ractopamine.
Operate according to embodiment 1 identical method of operating, utilize as can be known the test strips of present embodiment, can realize that the associating of salbutamol and Ractopamine quantitatively detects.
The above embodiment has only expressed several embodiment of the present utility model, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the utility model claim.Should be pointed out that for the person of ordinary skill of the art without departing from the concept of the premise utility, can also make some distortion and improvement, these all belong to protection domain of the present utility model.Therefore, the protection domain of the utility model patent should be as the criterion with claims.
Claims (4)
1. combination assay reagent card, it is characterized in that, comprise base plate, with the supporting loam cake of base plate, and card cap, offer at least two pickup grooves of mutually separating on the described base plate, be provided with a test strips in each pickup groove, as a result indicating window and test item indicating window are offered respectively in the position that described loam cake is corresponding with every test strips; One distal process of described test strips goes out, and inserts in the described card cap.
2. combination assay reagent card according to claim 1 is characterized in that, the pickup groove of offering on the described base plate is three.
3. combination assay reagent card according to claim 1 and 2 is characterized in that, described test strips is the colloidal gold immunochromatographydetection detection test paper bar, is provided with detection zone and control zone on it, the corresponding described detection zone of described as a result indicating window and control zone.
4. combination assay reagent card according to claim 3 is characterized in that, is provided with the test item indicator on the described test strips, the corresponding described test item of described test item indicating window indicator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220407441 CN202735346U (en) | 2012-08-16 | 2012-08-16 | Muti-index joint inspection reagent card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220407441 CN202735346U (en) | 2012-08-16 | 2012-08-16 | Muti-index joint inspection reagent card |
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| Publication Number | Publication Date |
|---|---|
| CN202735346U true CN202735346U (en) | 2013-02-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201220407441 Expired - Lifetime CN202735346U (en) | 2012-08-16 | 2012-08-16 | Muti-index joint inspection reagent card |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106153875A (en) * | 2016-08-19 | 2016-11-23 | 安徽贝驰生物科技有限公司 | A kind of portable reagent detection box |
| CN106526170A (en) * | 2016-12-30 | 2017-03-22 | 天津灵卫科技发展有限公司 | Portable lean meat powder detection kit |
| CN112362648A (en) * | 2020-10-30 | 2021-02-12 | 厦门波耐模型设计有限责任公司 | Integrated test paper and preparation process thereof |
-
2012
- 2012-08-16 CN CN 201220407441 patent/CN202735346U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106153875A (en) * | 2016-08-19 | 2016-11-23 | 安徽贝驰生物科技有限公司 | A kind of portable reagent detection box |
| CN106153875B (en) * | 2016-08-19 | 2018-12-04 | 安徽贝驰生物科技有限公司 | A kind of portable reagent detection box |
| CN106526170A (en) * | 2016-12-30 | 2017-03-22 | 天津灵卫科技发展有限公司 | Portable lean meat powder detection kit |
| CN112362648A (en) * | 2020-10-30 | 2021-02-12 | 厦门波耐模型设计有限责任公司 | Integrated test paper and preparation process thereof |
| CN112362648B (en) * | 2020-10-30 | 2021-11-26 | 厦门波耐模型设计有限责任公司 | Integrated test paper and preparation process thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130213 |
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| CX01 | Expiry of patent term |