CN101086497A

CN101086497A - TORCH five-item combined detection card

Info

Publication number: CN101086497A
Application number: CN 200610044607
Authority: CN
Inventors: 杨致亭; 刘玉华; 单永德; 吴瑞华; 李兰芬
Original assignee: 杨致亭
Priority date: 2006-06-09
Filing date: 2006-06-09
Publication date: 2007-12-12

Abstract

A TORCH five item compound inspection card comprises the card body, card cover and inspection bar, with the body installed with positioning plate and positioning slot, with hollow slots between positioning plates, inspection bar set inside the hollow slot and positioning slot, body connected with card cover through the linking device, lower end of the card cover having sample hole, top with testing item display hole with result observation hole between them, bottom of the inspection bar having plastic liner plate, top of the inspection having water absorption paper, whose surface bind with testing mark, lower of the water absorbing paper connected to the celluloid film, whose lower end connected to the top end of the gold marker, lower end of the inspection bar having sample cushion, whose top end connected with the lower end of the solid gold marker. It is simple, free from any instrument, applicable for clinical sample and physical inspection.

Description

A kind of TORCH five-item combined detection card

Technical field

The present invention relates to a kind of prenatal and postnatal care test card, specifically, relate to a kind of TORCH five-item combined detection card.

Background technology

To be American scholar will cause that intrauterine fetal infection cause miscarriage with several pregnant woman after ill in 1971 to TORCH one speech, even cause first primary and secondary of birth defect or dysplastic pathogen English name to combine, it comprises arc worm, rubella virus, cytomegalovirus, herpes simplex virus and other virus, torch infection can cause the pregnant woman to miscarry, fetal anomaly, it is the higher teratogenesis microorganism of the incidence of disease, infection is to the women, children threaten greatly, many countries have been detected TORCH as conventional examination project of pregnancy period, at present, detection with respect to pregnant woman and fetus exists sizable disadvantage at home, the one, need certain technical equipment condition, the 2nd, spended time is longer, expense is bigger, inconvenience very, can not extensively launch in all pregnant woman, this causes certain shortcoming for family planning and the prenatal and postnatal care that China advocated.

Summary of the invention

The purpose of this invention is to provide both simple to operate, do not need any instrument, be applicable to a kind of TORCH five-item combined detection card of the needs of fast detecting such as clinical samples, health check-up again.

For achieving the above object, the technical solution used in the present invention is: a kind of TORCH five-item combined detection card, comprise the card body, Ka Gai and test-strips, fixedly install some spacers and five locating slots on the described card body, have between described each spacer and five corresponding dead slots of locating slot, fixedly install coupling arrangement on the described card body, body is detachable is connected with card by coupling arrangement for described Ka Gai, described Ka Gai lower end is provided with five sample wells, described card covers end and is provided with five test item display aperture, is provided with five viewports as a result between described sample well and the test item display aperture.

Below be further improvement in the technical proposal of the present invention:

The quantity of described test-strips is five, and the lower end of described test-strips is placed on respectively in the corresponding locating slot, and the upper end of described test-strips is stuck in the dead slot.

The bottom of described test-strips is provided with plastics lining board, the upper end on described test-strips surface is provided with thieving paper, the outside surface of described thieving paper is provided with the test item sign, the middle part on described test-strips surface is provided with the nitrocellulose filter that solid phase has detection line and control line, the lower end of described thieving paper has detection line to be connected with the upper end of the nitrocellulose filter of control line with solid phase, the lower end on described test-strips surface is provided with sample pad, described solid phase has the nitrocellulose filter bottom of detection line and control line to be provided with solid phase gold label, the upper end of described sample pad is connected with the lower end of solid phase gold label, and described solid phase has detection line to be connected with the upper end of solid phase gold label with the lower end of the nitrocellulose filter of control line.

Five sample wells that described card covers are corresponding with the position of sample pad on every test-strips respectively, described card cover five as a result viewport respectively with every test-strips on solid phase have the position of nitrocellulose filter of detection line and control line corresponding, five test item display aperture that described card covers are corresponding with the position of test item sign on every test-strips respectively.

Beneficial effect: the present invention adopts above technical scheme, for the detection of TORCH virus, once measures, and five go out effect simultaneously, and is not only simple to operate, but also do not need any instrument, also is applicable to the needs of fast detecting such as clinical samples, health check-up.

The invention will be further described below in conjunction with drawings and Examples.

Description of drawings

Accompanying drawing 1 is the structural representation of the embodiment of the invention;

Accompanying drawing 2 is the structural representation of card body in the embodiment of the invention;

Accompanying drawing 3 is the structural representation of Ka Gai in the embodiment of the invention;

Accompanying drawing 4 is the structural representation of detector bar in the embodiment of the invention.

Embodiment

Embodiment, as shown in Figure 1, a kind of TORCH five-item combined detection card, comprise card body 1, card covers 2 and test-strips 8, as shown in Figure 2, fixedly install some spacers 6 and five locating slots 9 on the described card body 1, have between described each spacer 6 and five locating slots, 9 corresponding dead slots 17, fixedly install coupling arrangement 7 on the described card body 1, described card lid 2 is by coupling arrangement 7 and card 1 detachable connection of body, as shown in Figure 3, described card covers 2 lower ends and is provided with five sample wells 5, described card covers 2 upper ends and is provided with five test item display aperture 3, be provided with five viewports 4 as a result between described sample well 5 and the test item display aperture 3, the quantity of described test-strips 8 is five, the lower end of described test-strips 8 is placed on respectively in the corresponding locating slot 9, the upper end of described test-strips 8 is stuck in the dead slot 17, as shown in Figure 4, the bottom of described test-strips 8 is provided with plastics lining board 10, the upper end on described test-strips 8 surfaces is provided with thieving paper 11, the outside surface of described thieving paper 11 is provided with test item sign 12, the middle part on described test-strips 8 surfaces is provided with the nitrocellulose filter 13 that solid phase has detection line and control line, the lower end of described thieving paper 11 has detection line to be connected with the upper end of the nitrocellulose filter 13 of control line with solid phase, the lower end on described test-strips 8 surfaces is provided with sample pad 16, described solid phase has nitrocellulose filter 13 bottoms of detection line and control line to be provided with solid phase gold label 14, the upper end of described sample pad 16 is connected with the lower end of solid phase gold label 14, described solid phase has detection line to be connected with the upper end of solid phase gold label 14 with the lower end of the nitrocellulose filter 13 of control line, five sample wells 5 on the described card lid 2 are corresponding with the position of sample pad 16 on every test-strips 8 respectively, on the described card lid 2 five as a result viewport 4 respectively with every test-strips 8 on solid phase have the position of nitrocellulose filter 13 of detection line and control line corresponding, five test item display aperture 3 on the described card lid 2 are corresponding with the position of test item sign 12 on every test-strips 8 respectively, this kit utilizes the nanoscale colloidal gold particle tracer that serves as a mark, use the TORCH gene recombinant antigens, in conjunction with monoclonal antibody, according to gold-marking immunity chromatography test principle, detect the TORCH-IgM antibody among the human serum sample, after blood serum sample splashes into sample well 5 in the test process, sample moves on slowly along sample pad 16, and reaction system begins to start:

1, feminine gender: if do not have corresponding TORCH-TgM antibody in the tested sample, the golden labeling antibody in the reaction system can't form indirect antigen precursor reactant with the TORCH antigen of bag quilt, promptly occurs at the detection zone position of nitrocellulose filter 13 redfree response line; Golden labeling antibody in the reaction system continues to climb to test paper is disconnected, during to the control line position of nitrocellulose filter 13, golden labeling antibody promptly with the how anti-reaction bonded of anti-mouse TgG of bag quilt, form a red deposition reaction line---control line; This shows no TORCH-TgM antibody in the sample, negative result; Illustrate that simultaneously test paper W-response system works is normal.

2, the positive: if having TORCH-TgM antibody in the test sample, then TORCH-TgM antibody is earlier marked anti-people IgM monoclonal antibody with the gold of reaction system and is reacted, form golden labeling antibody--the TORCH-TgM antibody complex, this compound continues to creep, again with the TORCH antigen-reactive of the detection zone bag quilt of nitrocellulose filter 13,--immune complex of TORCH-TgM antibody-TORCH antigen has a red deposition reaction line at detection zone---detection line occurs to form golden labeling antibody; Simultaneously, excessive gold mark thing continue to climb with nitrocellulose filter 13 on the many resistive connections of anti-mouse TgG of check plot bag quilt close, form the red deposition reaction line of second---control line in the check plot; This shows TORCH antigen in the test sample, positive result.

The present invention is for the detection of TORCH infected by microbes, both simple to operate, reaction fast, visual result, do not need any large-scale instrument and equipment again, be applicable to the needs of qualitative fast detecting such as clinical samples, health check-up examination.

Claims

1, a kind of TORCH five-item combined detection card, comprise card body (1), Ka Gai (2) and test-strips (8), it is characterized in that: fixedly install some spacers (6) and five locating slots (9) on the described card body (1), have between described each spacer (6) and the corresponding dead slots of five locating slots (9) (17), fixedly install coupling arrangement (7) on the described card body (1), described Ka Gai (2) is by coupling arrangement (7) and card detachable connection of body (1), described Ka Gai (2) lower end is provided with five sample wells (5), described Ka Gai (2) upper end is provided with five test item display aperture (3), is provided with five viewports (4) as a result between described sample well (5) and the test item display aperture (3).

2, a kind of TORCH five-item combined detection card as claimed in claim 1, it is characterized in that: the quantity of described test-strips (8) is five, the lower end of described test-strips (8) is placed on respectively in the corresponding locating slot (9), and the upper end of described test-strips (8) is stuck in the dead slot (17).

3, a kind of TORCH five-item combined detection card as claimed in claim 2, it is characterized in that: the bottom of described test-strips (8) is provided with plastics lining board (10), the upper end on described test-strips (8) surface is provided with thieving paper (11), the outside surface of described thieving paper (11) is provided with test item sign (12), the middle part on described test-strips (8) surface is provided with solid phase the nitrocellulose filter of detection line and control line (13), the lower end of described thieving paper (11) has detection line to be connected with the upper end of the nitrocellulose filter (13) of control line with solid phase, the lower end on described test-strips (8) surface is provided with sample pad (16), described solid phase has nitrocellulose filter (13) bottom of detection line and control line to be provided with solid phase gold label (14), the upper end of described sample pad (16) is connected with the lower end of solid phase gold label (14), and described solid phase has detection line to be connected with the upper end of solid phase gold label (14) with the lower end of the nitrocellulose filter (13) of control line.

4, as claim 2 or 3 described a kind of TORCH five-item combined detection cards, it is characterized in that: five the sample wells (5) on the described Ka Gai (2) are corresponding with the position of sample pad (16) on every test-strips (8) respectively, on the described Ka Gai (2) five as a result viewport (4) go up solid phase with every test-strips (8) respectively and have the position of nitrocellulose filter (13) of detection line and control line corresponding, five the test item display aperture (3) on the described Ka Gai (2) respectively with every test-strips (8) on test item to identify the position of (12) corresponding.