CN203101391U - Gold-labeled immunochromatography detecting system - Google Patents
Gold-labeled immunochromatography detecting system Download PDFInfo
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- CN203101391U CN203101391U CN 201220594561 CN201220594561U CN203101391U CN 203101391 U CN203101391 U CN 203101391U CN 201220594561 CN201220594561 CN 201220594561 CN 201220594561 U CN201220594561 U CN 201220594561U CN 203101391 U CN203101391 U CN 203101391U
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- colorimetric device
- immunity chromatography
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Abstract
The utility model relates to the field of immunochromatography detecting techniques, and specifically relates to a gold-labeled immunochromatography detecting system. The gold-labeled immunochromatography detecting system comprises immunochromatography reaction devices and a movable standard color comparator which are connected with each other, wherein the immunochromatography reaction devices can be separated from each other along a connection part. The immunochromatography reaction devices respectively comprise a piece of immunochromatography detecting test paper and a shell, wherein the immunochromatography detecting test paper consists of a reaction upholder, a sample pad, a gold-labeled pad, a signal detecting pad and a water absorption pad. The movable standard color comparator comprises a standard color comparison card and a shell, wherein the standard color comparison card has 3-10 reference color bars which gradually change from top to bottom in the aspect of color; the reference color bars are transversely arrayed on the standard color comparison card in parallel; and corresponding analyte concentration ranges are marked at the right sides of the reference color bars. The gold-labeled immunochromatography detecting system provided by the utility model is capable of effectively avoiding the visual interference and helping the operators to rapidly and correctly judge the concentration of the determinand in the samples.
Description
Technical field
The utility model relates to the immuno-chromatographic assay technology field, is specifically related to a kind of gold-marking immunity chromatography detection system with packaged type standard colorimetric device.
Background technology
Immunochromatography is a kind of express-analysis technology that grows up early 1980s, combining, be prepared into golden labeling antibody bacterial detection surface antigen from Faulk in 1971 and Taylon with colloid gold particle with rabbit anti-salmonella antiserum distributes, colloidal gold technique is constantly developed and ripe over 30 years, after be introduced into the immunodiagnosis field.The collaurum quick diagnosis technology of using in the medical test mainly contains two kinds at present: collaurum fast immune chromatographic method (Colloidal gold enhanced immunochromatography assay, GICA) and dot immunogold filtration assay (Dot-immuno gold filtration assay, DIGFA).The ultimate principle of these two kinds of methods all is to be carrier with the miillpore filter, bag is by known antigens or antibody, after adding sample to be checked, through the capillarity of filter membrane or transudation the antibody or the antigen of bag quilt on antigen in the sample or antibody and the film are combined, reach testing goal with collaurum bond mark again.Collaurum is used labeling of monoclonal antibody mostly, and the non-specific adsorption effect is little, so have higher specificity.Detect generally, compare other method (need 1 ~ 2h as ELISA, PCR needs the time longer) and shortened detection time greatly as long as 5 ~ 10min will go out the result.Testing result directly shows by color, naked eyes are judged easily, do not need special instruments and equipment, only need test strips or diafiltration kit, as long as sample is done very simple processing or do not needed to do pre-treatment (can be tissue fluid, serum, ight soil and urine etc.).In addition, colloid gold reagent is highly stable, not influenced by extraneous factor such as temperature, thereby can even open-airly carry out the detection of various diseases in hospital, family, office space, but also long preservation of testing result.Collaurum quick diagnosis technology relies on characteristics such as it is convenient and swift, sensitive efficiently, stability is strong, and it is promoted rapidly in diagnostic field.
The collaurum quick detection reagent mostly is qualitative or half-quantitative detection greatly, along with the continuous development of clinical diagnosis demand, provides multinomial joint inspection and detection by quantitative result to become the important directions of colloidal gold immunochromatographimethod technical development.Existing on the market at present collaurum test card detection by quantitative instrument, main color intensity by the measurement test strip is converted into digital signal with color intensity, thereby realizes the detection by quantitative of analyte.Yet owing to medical level regional disparity, degree easy to detect and patient's reasons such as the state of an illness, qualitative or sxemiquantitative gold-marking immunity chromatography detection is still the most cheap at present, and medical institutions realize the most frequently used technological means of fast detecting.When realizing half-quantitative detection, producer is everlasting to detect and is equipped with a standard color comparison card in the packing box, and standard color comparison card is distributed with from top to bottom that color is deepened successively or some reference standard colour bands of the indication concentration range that shoals.After test card showed test strip, the reviewer carried out color contrast with reference to standard colour band and test strip, find out and the immediate standard colour band of test strip color, thus the half-quantitative detection result of acquisition analyte.When carrying out color contrast, reviewer's vision is subject to the interference of the adjacent colour band in standard colour band both sides that conforms to the result, tends to that the reviewer is accurately obtained testing result fast and exerts an influence.
Summary of the invention
The utility model provides a kind of gold-marking immunity chromatography detection system that can effectively avoid vision to disturb at the deficiencies in the prior art, and purpose is to help the operator to judge the concentration of determinand in the sample fast and accurately.
The utility model is achieved through the following technical solutions:
A kind of gold-marking immunity chromatography detection system comprises immunochromatography reaction unit and packaged type standard colorimetric device.
Described immunochromatography reaction unit and packaged type standard colorimetric device are connected to each other, and described packaged type standard colorimetric device can separate with the immunochromatography reaction unit along connecting portion.
Described immunochromatography reaction unit comprises immunity chromatography detection test paper and immunochromatography reaction unit shell, and described packaged type standard colorimetric device comprises standard color comparison card and standard colorimetric device shell.
Described immunity chromatography detection test paper comprises sample region of acceptance, mark zone, detection reaction district, suction zones, and sample pad of overlap joint, gold mark pad, signal detection pad, adsorptive pads are formed mutually successively by the reaction stilt and on described reaction stilt for they.Have overlapping between sample pad, gold mark pad, the adjacent each several part of signal detection pad with adsorptive pads, its effect is in order to guarantee that the chromatography effect carries out to the adsorptive pads position smoothly from sample pad, sample solution and sample pad are fully reacted, buffer system on the sample pad can in and the potential of hydrogen of sample solution, guarantee that the effective ingredient in the sample solution reacts smoothly with labelled antibody or antigen on the gold mark pad.
Nearly mark zone one side in described detection reaction district and nearly suction zones one side contain detection line (T line) and nature controlling line (C line) respectively, described detection line be coated with can with the antibody or the antigen of analyte response in the sample.
Described standard color comparison card have 3 ~ 10 from top to bottom color gradually change with reference to colour band, described with reference to the horizontal parallel arrangement of lower limb on colour band and the standard colorimetric device shell on standard color comparison card, the described sign with reference to the colour band right side has the corresponding analytes concentration range.Described standard color comparison card upper and lower side exceeds lower limb on the standard colorimetric device shell, the standard color comparison card that exceeds part can be respectively from the standard colorimetric device shell lower limb be folded back against on the standard colorimetric device shell back side.
Described immunochromatography reaction unit shell comprises upper shell and lower house, and the corresponding position in the sample region of acceptance of described upper shell and immunity chromatography detection test paper and detection reaction district is respectively equipped with well and view window; Described lower house central authorities are provided with and vertical draw-in groove of immunity chromatography detection test paper size coupling; Lower limb is respectively equipped with the import and the outlet of mating with the standard color comparison card width on the described standard colorimetric device shell, the position parallel with the immunity chromatography detection test paper detection line, the front of described standard colorimetric device shell is provided with reference to colour band form and analyte concentration scope form, the top of described standard colorimetric device outer casing frontispiece is provided with analyte title marked area, and the bottom is provided with patient information marked area and testing result marked area.
The utility model each several part is formed with function as follows:
Described reaction stilt is selected PVC plate or strip plastics lamina membranacea for use, plays fixing each ingredient of test strips of supporting, best surfaces has viscosity.
The material of described sample pad is selected from polyester film, water absorptivity glass fibre or filter paper fibre, works to absorb sample solution and buffered samples pH value of solution value.
Described gold mark pad can be made by one or more materials that are selected from polyester film, glass fibre or the filter paper fibre, being coated with the bond of antibody or antigen and collaurum on it, is the analyte in the sample solution and the place of golden labeling antibody or antigen generation specific reaction.In colloid gold label antibody or antigen process, also can adopt biotin-avidin or biotin-Streptavidin system to improve detection sensitivity.Described sample is selected from or derives from agricultural product, microbial product and biological specimen, and described biological specimen comprises blood, serum, urine, ight soil and sputum.
Described signal detection pad can select for use nitrocellulose filter or miillpore filter to make, preferred nitrocellulose filter, and main effect is that reaction result is come out with macroscopic characterization.
Described adsorptive pads can be selected the hygroscopicity fibre rete for use, and as filter paper, its effect is that the unnecessary sample solution that will move up absorbs.
Described immunochromatography reaction unit shell is made by hard material, preferred plastics, and it can more convenient reviewer be operated, and reduces the accuracy of sample size and assurance testing result.
Described standard color comparison card can be made by paper material, and it mainly acts on is to utilize to carry out color contrast with reference to colour band and test strip on it, helps the concentration range of decision analysis thing.
Described standard colorimetric device shell can be made by hard material or soft material, the preferred plastics of hard material, and the preferred papery of soft material, its effect is once only to show one with reference to colour band, and covers other with reference to colour band, avoids color contrast is produced interference.
The ultimate principle that the utility model detects analyte (is example with antigen) in the sample is: sample will move to adsorptive pads along test strips owing to capillarity, when moving to the gold mark pad that is coated with golden labeling antibody, target antigen and golden labeling antibody generation specific bond in the sample, when moving to detection line, corresponding antigens combines with the capture antibody at detection line place again in the sample, therefore collaurum-antibody-antigenic compound is stranded on the detection line, and the detection line place shows aubergine; On the contrary, if there is not target antigen in the sample to be tested, golden labeling antibody just not can with the capture antibody generation specific bond on the detection line, collaurum can not be trapped on the detection line, only shows a Quality Control band this moment.Be directly proportional with the antigen concentration size according to the shade of detection line place band and realize sxemiquantitative or detection by quantitative target antigen in the sample.
Compared with prior art, the gold-marking immunity chromatography detection system beneficial effect of realizing according to such scheme is mainly reflected in:
1. when the interpretation testing result, can only expose one with reference to colour band by what be provided with on the colorimetric device shell with reference to the colour band form, and be presented at form side by side with reference to colour band the right with reference to the concentration value of colour band correspondence, other are hidden by the part beyond the colorimetric device shell form with reference to colour band on the standard color comparison card, effectively avoid the vision of adjacent colour band to disturb, help obtaining only with reference to colour band, thereby help accurately to determine testing result, for the doctor patient diagnosed state of an illness and formulation therapeutic regimen provide reliable test basis.
But 2. record patient information and testing result on the colorimetric device, detect finish after, the preservation of colorimetric device can being torn along the position that be connected with the immunochromatography reaction unit has effectively guaranteed the correspondence one by one of testing result and to the preservation of patient's testing result.
3. use is very flexible, and the immunochromatography reaction unit that separates with colorimetric device also can place the detection by quantitative instrument to detect to obtain the detection by quantitative result.
Description of drawings
Fig. 1 is the immunity chromatography detection test paper structural representation of the utility model embodiment;
Fig. 2 is the structural representation of the utility model embodiment;
Fig. 3 is the standard color comparison card structural representation of the utility model embodiment.
Embodiment
Below in conjunction with the accompanying drawing of the utility model embodiment, the technical scheme among the utility model embodiment is clearly and completely described.Apparently, the embodiment described in the accompanying drawing only is preferred embodiment of the present utility model, for those of ordinary skills, under the prerequisite of not paying creative work, can also obtain other accompanying drawing according to described accompanying drawing.
Embodiment 1 structure of the present utility model
Fig. 1, Fig. 2 and Fig. 3 detail display structure of the present utility model.As can be seen from Figure 2, the utility model is made of immunochromatography reaction unit and packaged type standard colorimetric device two parts.
The immunochromatography reaction unit
The immunochromatography reaction unit is assembled by immunity chromatography detection test paper and immunochromatography reaction unit shell.
As seen from Figure 1, immunity chromatography detection test paper comprises the sample pad 2 that overlaps successively from left to right, gold mark pad 3, nitrocellulose filter 4 and the adsorptive pads 5 that is coated with golden labeling antibody on strip base plate 1.Base plate 1 middle part is a nitrocellulose filter 4, described nitrocellulose filter 4 nearly gold mark pad 3 one sides and nearly adsorptive pads 5 one sides contain 6 and nature controlling lines of a detection line (T line) (C line) 7 respectively, be coated with on the described detection line 6 can with the capture antibody that collaurum-antibody-the antigenic compound specificity combines.At base plate 1 right-hand member head is adsorptive pads 5, and the left end head is a sample pad 2, and nitrocellulose filter 4 two ends overlap mutually with gold mark pad 3 with adsorptive pads 5 respectively and are connected, and are pressed with sample pad 2 on gold mark pad 3.
Immunochromatography reaction unit shell 8 comprises upper shell and lower house, engages by the raised structures of upper shell and the shrinkage pool of lower house respectively.Described upper shell is provided with opening in the sample pad 2 that detects test paper and the correspondence position of nitrocellulose filter 4 respectively, is respectively to be used to drip the well 9 of sample and to observe the view window 10 of testing result.The central authorities of lower house have the strip groove with described immunity chromatography detection test paper coupling, are used to place colloidal gold immunochromatographydetection detection test paper.The outside surface of described upper shell indicates " S " printed words below well 9, indicate " T " and " C " printed words respectively in view window 10 left sides corresponding to detection line 6 and nature controlling line 7.
Packaged type standard colorimetric device
Packaged type standard colorimetric device is assembled by standard color comparison card and packaged type standard colorimetric device shell.
Standard color comparison card 11 be can be in the colorimetric device shell colorimetric card of pull up and down flexibly.As shown in Figure 3, standard color comparison card 11 have 5 from top to bottom color shoal gradually with reference to colour band 12, every all indicates corresponding concentration range with reference to colour band in concentration range marked area, right side 13.Described with reference to the horizontal parallel arrangement of lower limb on colour band 12 and the colorimetric device shell on standard color comparison card 11.
Colorimetric device outer casing frontispiece and left side detection line 6 corresponding positions are provided with reference to colour band form 14 and concentration range form 15, are used for observing the concentration range with reference to colour band and correspondence of colorimetric card.Lower limb is provided with the import and the outlet of mating with the standard color comparison card width on the described colorimetric device shell, and standard color comparison card is assembled into the colorimetric device from import and outlet.The top of described colorimetric device outer casing frontispiece is provided with analyte title marked area 16, is used to indicate the concrete analysis thing that is detected.The bottom of described colorimetric device outer casing frontispiece is provided with patient information marked area and testing result marked area, is used for record patient ID numbering and this patient's testing result.
The standard color comparison card upper and lower side exceeds the colorimetric device shell, length is about colorimetric device outer cover length twice, when non-detection status, the part that the colorimetric card upper and lower side exceeds can be folded back and be pasted on the colorimetric device shell back side, the part that will exceed is opened from the shell back side in use, utilizes the part that exceeds to move up and down.
Embodiment 2 preparation methods of the present utility model
Sample pad, gold mark pad, the nitrocellulose filter, the adsorptive pads that are coated with capture antibody and sheep anti-mouse igg antibody at nearly gold mark pad end and nearly adsorptive pads end respectively be pasted on successively obtain immunity chromatography detection test paper on the base plate; Immunity chromatography detection test paper is placed vertical draw-in groove of lower house, and upper shell and lower house obtain immuno-chromatography detection device by projection and shrinkage pool engaging fastening.Standard color comparison card is placed standard colorimetric device shell, and the part that the colorimetric card two ends exceed is folded back and is pasted on the colorimetric device shell back side.Standard colorimetric device and immunochromatography reaction unit are bonded together.If colorimetric device shell and immunochromatography reaction unit shell itself are one, then do not need to carry out again bonding.
Embodiment 3 uses the utility model to detect the method for antigen concentration in the sample
Operating personnel drip an amount of sample in immunochromatography reaction unit well place, waiting for 5 ~ 15 minutes fully reacts sample and test paper, after reaction finishes, can pass through pull colorimetric card up and down, make the test strip color that occupy with reference in the colour band form the most approaching with reference to colour band and reaction unit view window place, the concentration range that mark by analyte concentration scope form correspondence this moment can draw the content that detects analyte in the sample, and resulting concentration value is inserted the testing result marked area.When in the sample during driftlessness antigen, the detection reaction district of test paper only has a nature controlling line to become aubergine, and is negative.When the nature controlling line of test paper not during Show Color, represent that then misoperation, test paper may be expired or other problem occurs, this moment, testing result was invalid, need detect again.
It should be noted that at last: the above only is preferred embodiment of the present utility model, be not limited to the utility model, although the utility model is had been described in detail with reference to previous embodiment, for a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment put down in writing, and perhaps part technical characterictic wherein is equal to replacement.All within spirit of the present utility model and principle, any modification of being done, be equal to replacement, improvement etc., all should be included within the protection domain of the present utility model.
Claims (6)
1. a gold-marking immunity chromatography detection system is characterized in that, comprises immunochromatography reaction unit and packaged type standard colorimetric device; Described immunochromatography reaction unit comprises immunity chromatography detection test paper and immunochromatography reaction unit shell, described immunity chromatography detection test paper comprises sample region of acceptance, mark zone, detection reaction district, suction zones, nearly mark zone one side in described detection reaction district and nearly suction zones one side contain detection line and nature controlling line respectively, described detection line be coated with can with the antibody or the antigen of analyte response in the sample; Described packaged type standard colorimetric device comprises standard color comparison card and standard colorimetric device shell, described standard color comparison card have 3～10 from top to bottom color gradually change with reference to colour band, the described sign with reference to the colour band right side has the corresponding analytes concentration range, and the position parallel with the immunity chromatography detection test paper detection line, the front of described standard colorimetric device shell is provided with reference to colour band form and analyte concentration scope form.
2. gold-marking immunity chromatography detection system according to claim 1, it is characterized in that, described immunochromatography reaction unit and packaged type standard colorimetric device are connected to each other, and described packaged type standard colorimetric device can separate with the immunochromatography reaction unit along connecting portion.
3. gold-marking immunity chromatography detection system according to claim 1, it is characterized in that form mutually successively by the sample pad of overlap joint, gold mark pad, signal detection pad, adsorptive pads by the reaction stilt and on described reaction stilt for described immunity chromatography detection test paper.
4. gold-marking immunity chromatography detection system according to claim 1 is characterized in that, described with reference to the horizontal parallel arrangement of lower limb on colour band and the standard colorimetric device shell on standard color comparison card.
5. according to claim 1 or 4 described gold-marking immunity chromatography detection systems, it is characterized in that, described standard color comparison card upper and lower side exceeds lower limb on the standard colorimetric device shell, the standard color comparison card that exceeds part can be respectively from the standard colorimetric device shell lower limb be folded back against on the standard colorimetric device shell back side.
6. gold-marking immunity chromatography detection system according to claim 1, it is characterized in that, described immunochromatography reaction unit shell comprises upper shell and lower house, and the corresponding position in the sample region of acceptance of described upper shell and immunity chromatography detection test paper and detection reaction district is respectively equipped with well and view window; Described lower house central authorities are provided with and vertical draw-in groove of immunity chromatography detection test paper size coupling; Lower limb is respectively equipped with the import and the outlet of mating with the standard color comparison card width on the described standard colorimetric device shell, the top of described standard colorimetric device outer casing frontispiece is provided with analyte title marked area, and the bottom is provided with patient information marked area and testing result marked area.
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| CN 201220594561 CN203101391U (en) | 2012-11-12 | 2012-11-12 | Gold-labeled immunochromatography detecting system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220594561 CN203101391U (en) | 2012-11-12 | 2012-11-12 | Gold-labeled immunochromatography detecting system |
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| CN203101391U true CN203101391U (en) | 2013-07-31 |
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| CN 201220594561 Expired - Lifetime CN203101391U (en) | 2012-11-12 | 2012-11-12 | Gold-labeled immunochromatography detecting system |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103983643A (en) * | 2014-06-03 | 2014-08-13 | 河南工业大学 | Kit for rapidly detecting calcium peroxide in wheat flour and detecting method of kit |
| CN106525838A (en) * | 2016-12-07 | 2017-03-22 | 百奥森（江苏）食品安全科技有限公司 | Prednisolone determination method and prednisolone determination card |
| CN106525837A (en) * | 2016-12-07 | 2017-03-22 | 无锡艾科瑞思产品设计与研究有限公司 | Natamycin determination method and natamycin determination card |
| CN107389925A (en) * | 2017-07-28 | 2017-11-24 | 上海交通大学医学院附属上海儿童医学中心 | The bigeminy colloidal gold immunochromatographykit kit of IL 6 and IL 10 for pyemia quick diagnosis |
| CN111505269A (en) * | 2020-04-30 | 2020-08-07 | 海口健康岛生物科技有限公司 | Immunochromatographic analysis instrument |
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2012
- 2012-11-12 CN CN 201220594561 patent/CN203101391U/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103983643A (en) * | 2014-06-03 | 2014-08-13 | 河南工业大学 | Kit for rapidly detecting calcium peroxide in wheat flour and detecting method of kit |
| CN106525838A (en) * | 2016-12-07 | 2017-03-22 | 百奥森（江苏）食品安全科技有限公司 | Prednisolone determination method and prednisolone determination card |
| CN106525837A (en) * | 2016-12-07 | 2017-03-22 | 无锡艾科瑞思产品设计与研究有限公司 | Natamycin determination method and natamycin determination card |
| CN107389925A (en) * | 2017-07-28 | 2017-11-24 | 上海交通大学医学院附属上海儿童医学中心 | The bigeminy colloidal gold immunochromatographykit kit of IL 6 and IL 10 for pyemia quick diagnosis |
| CN107389925B (en) * | 2017-07-28 | 2019-03-29 | 上海交通大学医学院附属上海儿童医学中心 | IL-6 and IL-10 bigeminy colloidal gold immunochromatographykit kit for pyemia quick diagnosis |
| CN111505269A (en) * | 2020-04-30 | 2020-08-07 | 海口健康岛生物科技有限公司 | Immunochromatographic analysis instrument |
| CN111505269B (en) * | 2020-04-30 | 2024-03-29 | 广东康盾创新产业集团股份公司 | Immunochromatography analysis instrument |
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