CN111505269B - Immunochromatography analysis instrument - Google Patents

Immunochromatography analysis instrument Download PDF

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Publication number
CN111505269B
CN111505269B CN202010360180.XA CN202010360180A CN111505269B CN 111505269 B CN111505269 B CN 111505269B CN 202010360180 A CN202010360180 A CN 202010360180A CN 111505269 B CN111505269 B CN 111505269B
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China
Prior art keywords
plate
groove
strip
color
test paper
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Application number
CN202010360180.XA
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Chinese (zh)
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CN111505269A (en
Inventor
湛振键
齐国光
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Qi Guoguang
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Guangdong Kangdun Innovation Industry Group Co ltd
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Priority to CN202010360180.XA priority Critical patent/CN111505269B/en
Publication of CN111505269A publication Critical patent/CN111505269A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302Apparatus specially adapted for immunological test procedures
    • G01N33/5304Reaction vessels, e.g. agglutination plates
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84Systems specially adapted for particular applications
    • G01N21/8483Investigating reagent band

Abstract

The invention provides an immunochromatography analysis instrument which comprises a bottom plate, a first vertical plate, a second vertical plate, an upper computer, a camera, an electric telescopic rod, a color comparison plate and a test paper clamping plate, wherein before an analysis test is carried out, test paper is fixed on the test paper clamping plate, after a sample is dripped and the test paper clamping plate is kept stand for a period of time, the test paper clamping plate is pushed into an acquisition cavity between the first vertical plate and the second vertical plate, the upper computer controls the electric telescopic rod to drive the color comparison plate to slide in a strip-shaped groove, so that different color areas on the color comparison plate move to the lower part of the acquisition cavity, after the camera acquires images of the test paper and the color areas of the color comparison plate, the upper computer carries out color matching, and when the color matching is successful, corresponding colors and corresponding concentration information are displayed on a screen of the test paper and the image information of the color comparison plate are acquired simultaneously, interference of the colors caused by illumination or air quality of the external environment is avoided, and errors generated in the analysis process are reduced.

Description

Immunochromatography analysis instrument
Technical Field
The invention relates to the technical field of immunodetection, in particular to an immunochromatography analysis instrument.
Background
Immunochromatography (immunochromatography) is a rapid diagnosis technology which has been developed in recent years, and the principle is that a specific antibody is fixed on a certain zone of a nitrocellulose membrane, after one end of the dried nitrocellulose membrane is immersed in a sample (urine or serum), the sample moves forward along the membrane due to capillary action, and when the sample moves to a zone where the antibody is fixed, the corresponding antigen in the sample specifically binds with the antibody, and if the zone is stained with immune colloidal gold or immune enzyme, the zone can display a certain color, thereby realizing specific immunodiagnosis.
At present, the concentration of a sample is generally judged according to the color type and the depth of a color development area, the adopted method is that a camera collects the color development area and then processes and judges the color development area through software, the mode has larger error, the contrast color stored in the software is obtained by testing in an ideal state, and when the camera collects the color development area, the camera is influenced by ambient environment illumination, air quality and the like, so that the color development area collected by the camera has larger access to the actual situation, the finally obtained concentration value of the sample has larger error, and the specific immunodiagnosis is not facilitated.
Disclosure of Invention
Therefore, the invention provides an immunochromatography analysis instrument, and simultaneously, the image information of the test paper and the colorimetric plate is collected for comparison, so that the influence of ambient illumination or air quality on the color is avoided, and the error is reduced.
The technical scheme of the invention is realized as follows:
the immunochromatography analysis instrument comprises a bottom plate, a first vertical plate, a second vertical plate, an upper computer, a camera, an electric telescopic rod, a colorimetric plate and a test paper clamping plate, wherein a strip-shaped groove is formed in the upper surface of the bottom plate, the colorimetric plate is arranged in the strip-shaped groove in a sliding mode, and the electric telescopic rod is arranged in the strip-shaped groove and connected with the colorimetric plate; the first vertical plate and the second vertical plate span over the strip-shaped groove and form an acquisition cavity, the camera is positioned above the acquisition cavity, the test paper clamping plate is positioned in the acquisition cavity, and the upper computer is arranged on the upper surface of the bottom plate; the color comparison plate comprises an elastic collision bead and a plurality of color areas, wherein the elastic collision bead is arranged on the upper surface of the color comparison plate, the elastic collision bead is positioned between the two color areas, a positioning groove is formed in the lower surface of the second vertical plate, which is positioned above the strip-shaped groove, and a first pressing button is arranged in the positioning groove; the upper computer is electrically connected with the camera, the electric telescopic rod and the first pressing button respectively.
Preferably, the lower surface of the colorimetric plate corresponding to each color area is provided with a second pressing button, the lower surface of the strip-shaped groove below the collecting cavity is provided with a retraction groove, a retraction spring, a metal rod and an electromagnet are arranged in the retraction groove, the lower bottom surface of the metal rod is connected with the retraction spring, the electromagnet is arranged in the retraction groove and is in contact with the metal rod, and the upper computer is respectively connected with the second pressing buttons and the electromagnet in a ferroelectric manner.
Preferably, a sliding groove is formed in the side face of the strip-shaped groove, a sliding strip is arranged on the side face of the colorimetric plate, and the sliding strip is located in the sliding groove.
Preferably, the bottom plate upper surface is provided with the spacing groove, the spacing groove is on a parallel with first riser to communicate to the bar groove, test paper cardboard lower surface is provided with spacing, spacing is located the spacing inslot.
Preferably, a limiting block is arranged at the joint of the limiting groove and the strip-shaped groove.
Preferably, a positioning column is arranged on the upper surface of the test paper clamping plate.
Preferably, the automatic adjusting device further comprises a clamping mechanism, a sliding block is arranged on the lower surface of the first vertical plate, a distance adjusting groove is formed in the upper surface of the bottom plate, a distance adjusting spring is arranged in the distance adjusting groove, the distance adjusting spring is connected with the sliding block, and the clamping mechanism clamps the distance adjusting spring.
Preferably, the clamping mechanism comprises a rotating rod, one end of the rotating rod penetrates into the distance adjusting groove from the side face of the bottom plate and is located above the distance adjusting spring, a clamping block is arranged on the rotating rod, and the rotating rod is rotated to enable the clamping block to be embedded into the distance adjusting spring to clamp the distance adjusting spring.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides an immunochromatography analysis instrument, which is characterized in that after test paper is fixed on a test paper clamping plate, a color comparison plate is driven to move in a strip-shaped groove through an electric telescopic rod, a camera acquires a color development area on the test paper and a color area of the color comparison plate and transmits image information to an upper computer, the upper computer compares the color development area and the color area of the color comparison plate, if the colors are not matched, the electric telescopic rod is controlled to drive the color comparison plate to move, so that different color areas move into a collection cavity, and as the camera simultaneously performs image collection on the color development area and the color area of the color comparison plate, the influence of factors such as illumination, air and the like on the color is avoided, the concentration error of a finally detected sample is ensured to be smaller, and the detection result is ensured.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only preferred embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic structural diagram of an immunochromatographic assay device of the present invention;
FIG. 2 is a schematic diagram showing a connection structure between a second riser and a bottom plate of an immunochromatographic assay device according to the present invention;
FIG. 3 is a schematic structural view of a colorimetric plate of an immunochromatographic assay device of the present invention;
FIG. 4 is a schematic view showing a connection structure between a first riser and a plate of an immunochromatographic assay device according to the present invention;
FIG. 5 is a schematic circuit diagram of an immunochromatographic assay device of the present invention;
in the figure, 1 is a bottom plate, 2 is a first vertical plate, 3 is a second vertical plate, 4 is an upper computer, 5 is a camera, 6 is an electric telescopic rod, 7 is a color comparison plate, 8 is a test paper clamping plate, 9 is a strip-shaped groove, 10 is a collection cavity, 11 is an elastic collision bead, 12 is a color area, 13 is a positioning groove, 14 is a first pressing button, 15 is a second pressing button, 16 is a retraction groove, 17 is a retraction spring, 18 is a metal rod, 19 is an electromagnet, 20 is a sliding groove, 21 is a sliding strip, 22 is a limit groove, 23 is a limit bar, 24 is a limit block, 25 is a positioning column, 26 is a sliding block, 27 is a distance-adjusting groove, 28 is a distance-adjusting spring, 29 is a rotating rod, and 30 is a clamping block.
Detailed Description
For a better understanding of the technical content of the present invention, a specific example is provided below, and the present invention is further described with reference to the accompanying drawings.
Referring to fig. 1 to 5, the immunochromatographic analysis instrument provided by the invention comprises a bottom plate 1, a first vertical plate 2, a second vertical plate 3, an upper computer 4, a camera 5, an electric telescopic rod 6, a colorimetric plate 7 and a test paper clamping plate 8, wherein a strip-shaped groove 9 is formed in the upper surface of the bottom plate 1, the colorimetric plate 7 is slidably arranged in the strip-shaped groove 9, and the electric telescopic rod 6 is arranged in the strip-shaped groove 9 and is connected with the colorimetric plate 7; the first vertical plate 2 and the second vertical plate 3 span over the strip-shaped groove 9 and form an acquisition cavity 10, the camera 5 is positioned above the acquisition cavity 10, the test paper clamping plate 8 is positioned in the acquisition cavity 10, and the upper computer 4 is arranged on the upper surface of the bottom plate 1; the color comparison plate 7 comprises an elastic collision bead 11 and a plurality of color areas 12, wherein the elastic collision bead 11 is arranged on the upper surface of the color comparison plate, the elastic collision bead 11 is positioned between the two color areas 12, a positioning groove 13 is arranged on the lower surface of the second vertical plate 3 above the strip-shaped groove 9, and a first pressing button 14 is arranged in the positioning groove 13; the upper computer 4 is electrically connected with the camera 5, the electric telescopic rod 6 and the first pressing button 14 respectively.
The immunochromatography analysis instrument is used for carrying out immunochromatography analysis on nitrocellulose membrane test paper to realize specific immunodiagnosis, after the test paper is fixed on a test paper clamping plate 8 before analysis, a sample (urine or serum) is dripped on the test paper, then the test paper is kept for a period of time, after the color appears on the test paper, the upper computer 4 controls the electric telescopic rod 6 to drive the color comparison plate 7 to move, so that a first color area 12 on the color comparison plate 7 moves to one side of the test paper, the camera 5 carries out image acquisition on the color comparison plate 7 and the test paper, acquired image information is transmitted to the upper computer 4, the upper computer 4 judges whether two colors are matched or not, if the colors are not matched, the upper computer 4 controls the electric telescopic rod 6 to drive the color comparison plate 7 to move further, so that a second color area 12 enters the acquisition cavity 10, then the camera 5 acquires images, and the images are repeated until the upper computer 4 judges that the two colors are matched, at the moment, the upper computer 4 can display corresponding color areas 12 on a screen of the test paper, the color comparison plate and the corresponding concentration information are displayed on the screen, the image information is acquired by the upper computer, and the color comparison plate is directly compared with the test paper, and the quality of the color comparison is reduced, and the color comparison is better, the quality of the color comparison is compared with the test paper, and the color comparison is better, and the quality is better compared with the color comparison, and the quality of the test paper is directly, and the image is simultaneously, and compared with the quality when the image is better when compared.
The invention provides a plurality of color areas 12 on a colorimetric plate 7, wherein the width of each color area 12 is equal to the width of a collecting cavity 10 formed by a first vertical plate 2 and a second vertical plate 3, and in the whole analysis process, the color areas 12 of the colorimetric plate 7 need to be moved below the collecting cavity 10, so that an electric telescopic rod 6 is arranged, controlled by an upper computer 4, and in order to ensure that each color area 12 can be completely moved below the collecting cavity 10, a positioning groove 13 is arranged on the lower surface of the second vertical plate 3, an elastic collision bead 11 is arranged on the upper surface of the colorimetric plate 7 between any two color areas 12, when the colorimetric plate 7 slides in the strip-shaped groove 9, the elastic collision bead 11 arranged on the upper surface of the colorimetric plate is embedded in the positioning groove 13, and a first pressing button 14 arranged in the positioning groove 13 is triggered, when the first button 14 is pressed, the upper computer 4 controls the electric telescopic rod 6 to stop working, so that the corresponding color area 12 on the colorimetric plate 7 can stay below the collecting cavity 10, meanwhile, a certain stay time is set in the upper computer 4, the stay time can be used for the camera 5 to collect image data and the upper computer 4 to carry out color matching judgment, if the upper computer 4 judges that the colors are not matched, the electric telescopic rod 6 is controlled to drive the colorimetric plate 7 to move in the strip-shaped groove 9, and the next color area 12 is enabled to move below the collecting cavity 10, so that the operation is repeated until the matched colors are obtained, the whole colorimetric process is free from manual participation, and an analyst only needs to wait for the result displayed by the upper computer 4 at one side, thereby realizing a complete automatic process.
Preferably, the lower surface of the colorimetric plate 7 corresponding to each color area 12 is provided with a second pressing button 15, the lower surface of the bar-shaped groove 9 below the collecting cavity 10 is provided with a retraction groove 16, a retraction spring 17, a metal rod 18 and an electromagnet 19 are arranged in the retraction groove 16, the metal rod 18 is arranged in the retraction groove 16, the lower bottom surface of the metal rod 18 is connected with the retraction spring 17, the electromagnet 19 is arranged in the retraction groove 16 and is in contact with the metal rod 18, and the upper computer 4 is respectively electrically connected with the second pressing buttons 15 and the electromagnet 19.
A reduction groove 16 is arranged at a position of the strip groove 9 below the collection cavity 10, a metal rod 18 is arranged in the reduction groove 16, the metal rod 18 is connected with the bottom of the reduction groove 16 through a reduction spring 17, meanwhile, an electromagnet 19 is further arranged in the reduction groove 16, the electromagnet 19 is in contact with the metal rod 18, in a normal state, the electromagnet 19 is electrified and magnetized so as to attract the metal rod 18, the metal rod 18 is kept in the reduction groove 16, when one color area 12 of the colorimetric plate 7 moves to the collection cavity 10, a second pressing button 15 correspondingly arranged on the lower surface of the colorimetric plate 7 is positioned right above the reduction groove 16 at the bottom of the strip groove 9, if the upper computer 4 judges that the color of the color area 12 is matched with the color of test paper, the upper computer 4 cuts off the electric energy output to the electromagnet 19 so that the electromagnet 19 is demagnetized, the metal rod 18 is ejected upwards under the action of the reduction spring 17, the second pressing button 15 is pressed, after the upper computer 4 receives an electric signal sent by the second pressing button 15, the corresponding color area 12 is directly displayed on a display, and the corresponding electric signal and the corresponding color area 12 and the corresponding color information corresponding to the color area 12 can not be judged according to the fact that the corresponding color area is not pressed by the second pressing button 12 is arranged on the surface area according to the second pressing button 12.
Preferably, a sliding groove 20 is arranged on the side surface of the strip-shaped groove 9, a sliding strip 21 is arranged on the side surface of the colorimetric plate 7, and the sliding strip 21 is positioned in the sliding groove 20.
When the color plate 7 slides in the strip-shaped groove 9, the sliding strips 21 arranged on the side edges of the color plate can slide along the sliding grooves 20 on the side surfaces of the strip-shaped groove 9.
Preferably, the upper surface of the bottom plate 1 is provided with a limit groove 22, the limit groove 22 is parallel to the first vertical plate 2 and communicated with the strip-shaped groove 9, the lower surface of the test paper clamping plate 8 is provided with a limit bar 23, the limit bar 23 is positioned in the limit groove 22, a limit block 24 is arranged at the joint of the limit groove 22 and the strip-shaped groove 9, and the upper surface of the test paper clamping plate 8 is provided with a positioning column 25.
According to the invention, the test paper clamping plate 8 is arranged into a sliding structure, before analysis, the test paper clamping plate 8 is pulled out of the collection cavity 10, a limiting strip 23 arranged on the lower surface of the test paper clamping plate is enabled to move along a limiting groove 22 in a direction away from the collection cavity 10, then test paper to be tested is fixed on the test paper clamping plate 8, a positioning column 25 arranged on the test paper clamping plate 8 is used for positioning the test paper, after a sample is dropped on the test paper, the test paper clamping plate 8 is pushed to slide along the limiting groove 22, after the limiting strip 23 is contacted with a limiting block 24 at the end part of the limiting groove 22, the test paper clamping plate 8 is stopped to be pushed, and then the test paper clamping plate 8 is positioned in the collection cavity 10 and can be subjected to immunochromatographic analysis test after standing for a period of time.
Preferably, the automatic adjusting device further comprises a clamping mechanism, the lower surface of the first vertical plate 2 is provided with a sliding block 26, the upper surface of the bottom plate 1 is provided with a distance adjusting groove 27, a distance adjusting spring 28 is arranged in the distance adjusting groove 27, the distance adjusting spring 28 is connected with the sliding block 26, the clamping mechanism clamps the distance adjusting spring 28, the clamping mechanism comprises a rotating rod 29, one end of the rotating rod 29 penetrates into the distance adjusting groove 27 from the side surface of the bottom plate 1 and is located above the distance adjusting spring 28, a clamping block 30 is arranged on the rotating rod 29, and the rotating rod 29 enables the clamping block 30 to be embedded into the distance adjusting spring 28 to clamp the distance adjusting spring 28.
In order to be compatible with the use of test papers with various sizes, the invention also sets the first vertical plate 2 to be in an adjustable structure, so that the width of the collection cavity 10 is changed, when the distance is adjusted, the first vertical plate 2 is pushed first, the sliding block 26 arranged on the lower surface of the first vertical plate is slid in the distance adjusting groove 27, the sliding block 26 can squeeze the distance adjusting spring 28 in the sliding process, after the position of the first vertical plate 2 is selected, the rotating rod 29 is rotated to be positioned at one end outside the bottom plate 1, after the clamping block 30 positioned on the outer surface of the rotating rod is rotated and embedded into the distance adjusting spring 28, the clamping of the distance adjusting spring 28 is realized, so that the first vertical plate 2 is fixed at a position which needs to be adjusted, and the colorimetric plates 7 with different sizes can be correspondingly analyzed after being replaced at the moment.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.

Claims (6)

1. The immunochromatography analysis instrument is characterized by comprising a bottom plate, a first vertical plate, a second vertical plate, an upper computer, a camera, an electric telescopic rod, a colorimetric plate and a test paper clamping plate, wherein the upper surface of the bottom plate is provided with a strip-shaped groove, the colorimetric plate is arranged in the strip-shaped groove in a sliding manner, and the electric telescopic rod is arranged in the strip-shaped groove and is connected with the colorimetric plate; the first vertical plate and the second vertical plate span over the strip-shaped groove and form an acquisition cavity, the camera is positioned above the acquisition cavity, the test paper clamping plate is positioned in the acquisition cavity, and the upper computer is arranged on the upper surface of the bottom plate; the color comparison plate is provided with a plurality of color areas, the width of each color area is equal to the width of the collection cavity formed by the first vertical plate and the second vertical plate, a positioning groove is formed in the lower surface of the second vertical plate above the strip-shaped groove, elastic collision beads are arranged on the upper surface of the color comparison plate between any two color areas, a first pressing button is arranged in the positioning groove, and when the color comparison plate slides in the strip-shaped groove, the elastic collision beads can be embedded into the positioning groove and trigger the first pressing buttons arranged in the positioning groove; the upper computer is electrically connected with the camera, the electric telescopic rod and the first pressing button respectively; the lower surface of the color comparison plate corresponding to each color area is provided with a second pressing button, the lower surface of the strip-shaped groove below the collecting cavity is provided with a retraction groove, a retraction spring, a metal rod and an electromagnet are arranged in the retraction groove, the metal rod is positioned in the retraction groove, the lower bottom surface of the metal rod is connected with the retraction spring, the electromagnet is arranged in the retraction groove and is contacted with the metal rod, and the upper computer is respectively connected with the second pressing button and the electromagnet in a ferroelectric manner; the test paper cardboard is characterized in that a limiting groove is formed in the upper surface of the bottom plate, is parallel to the first vertical plate and is communicated with the strip-shaped groove, and a limiting strip is arranged on the lower surface of the test paper cardboard and is located in the limiting groove.
2. The immunochromatographic assay device of claim 1, wherein the side of the strip-shaped groove is provided with a slide groove, and the side of the colorimetric plate is provided with a slide bar, and the slide bar is positioned in the slide groove.
3. The immunochromatographic assay device according to claim 1, wherein a stopper is provided at a junction of the stopper groove and the strip groove.
4. The immunochromatographic assay device of claim 1, wherein the upper surface of the test strip card is provided with a positioning column.
5. The immunochromatographic assay instrument according to claim 1, further comprising a clamping mechanism, wherein the lower surface of the first vertical plate is provided with a sliding block, the upper surface of the bottom plate is provided with a distance adjusting groove, a distance adjusting spring is arranged in the distance adjusting groove, the distance adjusting spring is connected with the sliding block, and the clamping mechanism clamps the distance adjusting spring.
6. The immunochromatographic assay device according to claim 5, wherein the clamping mechanism comprises a rotary rod, one end of the rotary rod penetrates into the distance-adjusting groove from the side surface of the bottom plate and is positioned above the distance-adjusting spring, a clamping block is arranged on the rotary rod, and the rotary rod is rotated to enable the clamping block to be embedded into the distance-adjusting spring to clamp the distance-adjusting spring.
CN202010360180.XA 2020-04-30 2020-04-30 Immunochromatography analysis instrument Active CN111505269B (en)

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Application Number Priority Date Filing Date Title
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CN111505269B true CN111505269B (en) 2024-03-29

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007285988A (en) * 2006-04-20 2007-11-01 Wako Pure Chem Ind Ltd Analyzing device and measuring method
JP2012225864A (en) * 2011-04-22 2012-11-15 Ushio Inc Analyzer
CN202275082U (en) * 2011-05-06 2012-06-13 杭州顿力医疗器械股份有限公司 CCD (Charge Coupled Device) type colloidal gold immunochromatograohic assay diagnostic strip quantitative analysis system
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CN203101391U (en) * 2012-11-12 2013-07-31 南京诺尔曼生物技术有限公司 Gold-labeled immunochromatography detecting system
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CN208457533U (en) * 2018-07-13 2019-02-01 深圳市虎硕科技开发有限公司 A kind of smart projector having roll adjustment function

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