CN110221054B - High-flux automatic chromatographic detection equipment - Google Patents
High-flux automatic chromatographic detection equipment Download PDFInfo
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- CN110221054B CN110221054B CN201910329541.1A CN201910329541A CN110221054B CN 110221054 B CN110221054 B CN 110221054B CN 201910329541 A CN201910329541 A CN 201910329541A CN 110221054 B CN110221054 B CN 110221054B
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 113
- 230000007246 mechanism Effects 0.000 claims abstract description 107
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 26
- 238000011161 development Methods 0.000 claims abstract description 7
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- 206010011409 Cross infection Diseases 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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Abstract
The invention discloses a high-flux automatic chromatography detection device, which comprises: a chromatographic device; the test strip pushing device is arranged above the chromatographic device and is used for driving the detection mechanism to slide along the outer wall of the liquid storage tank so that a sample pad of the test strip contacts with sample liquid flowing into each detection channel of the reaction cavity; the switching driving device is used for driving the switching mechanism to move so that the liquid storage tank is communicated with or cut off from each detection channel of the reaction cavity; the vibration device is used for driving the chromatographic device to vibrate; the code scanning device is used for collecting information of the identification codes arranged on the detection mechanism; the image acquisition device is used for acquiring an image of the color development area on the test strip; and the test strip pushing device, the code scanning device, the image acquisition device, the switching driving device and the oscillating device are respectively and electrically connected with the control device. The equipment provided by the invention can realize automatic sample feeding, automatic shaking and automatic detection, and the whole process does not need manual operation, so that the working efficiency is greatly improved.
Description
Technical Field
The invention relates to the technical field of biochemical detection, in particular to high-flux automatic chromatographic detection equipment.
Background
The chromatography detection is an important on-site rapid detection form in recent years, and is popular in the fields of clinical, food safety, environmental detection and the like due to convenience and rapidness. Currently, chromatography often requires the use of multiple test strips to perform multiple index assays on a single sample. For detection, it is common practice to place the sample fluid in a common chamber, then place a test strip, and read the results at a specific time.
However, in the process of implementing the embodiment of the present invention, the present inventors have found that at least the following technical problems exist in the above technology:
and (one) is only suitable for chromatography of a one-step method, and cannot be compatible with a two-step method. And the public cavity can pollute the detection process with high environmental requirements, and can not meet detection projects such as nucleic acid and the like.
And secondly, the user needs to monitor the reaction time in the detection process, stop the reaction at different time points for different projects, or enter the next reaction, and interpret the chromatographic result in a shorter time. Such time-desirability places high demands on operators, is prone to error when faced with multiple samples, and is also prone to missing points in time, resulting in inaccurate results.
And thirdly, the test strips can be read one by one, so that the timeliness of reading is influenced, and in order to improve the consistency of the reaction time, the sample pad fully sucked with the sample is required to be manually removed, so that the sample is easy to pollute while the operation is inconvenient. The reaction time is still inconsistent due to the residual sample liquid on the chromatographic membrane, and the accuracy of the result is reduced.
Disclosure of Invention
In order to make up for the defects of the prior art, the invention provides high-flux automatic chromatographic detection equipment.
The technical problems to be solved by the invention are realized by the following technical scheme:
a high throughput automated chromatography detection apparatus comprising:
the chromatographic device comprises a liquid storage tank, a reaction cavity, a switching mechanism and a detection mechanism; the liquid storage tank is used for containing sample liquid; the reaction cavity is arranged below the liquid storage tank and comprises at least two mutually independent detection channels; the switching mechanism is arranged between the liquid storage tank and the reaction cavity and used for controlling the liquid storage tank to be communicated with or cut off from each detection channel of the reaction cavity; the detection mechanism comprises a shell and a plurality of test strips which are arranged in the shell and are parallel to each other; the detection mechanism is arranged above the reaction cavity and is in sliding connection with the liquid storage tank;
The test strip pushing device is arranged above the chromatographic device and is used for driving the detection mechanism to slide along the outer wall of the liquid storage tank so that a sample pad of the test strip contacts with sample liquid flowing into each detection channel of the reaction cavity;
the switching driving device is arranged on one side of the chromatographic device and is used for driving the switching mechanism to move so that the liquid storage tank is communicated with or cut off from each detection channel of the reaction cavity;
the vibration device is connected with the chromatographic device and is used for driving the chromatographic device to vibrate;
the code scanning device is used for collecting information of the identification codes arranged on the detection mechanism;
the image acquisition device is used for acquiring an image of the color development area on the test strip;
and the test strip pushing device, the code scanning device, the image acquisition device, the switching driving device and the oscillating device are respectively and electrically connected with the control device.
Further, a longitudinal baffle plate is arranged in the reaction cavity, and the inner cavity of the reaction cavity is divided into a plurality of mutually independent detection channels by the longitudinal baffle plate; the top of reaction chamber corresponds every detection channel's position all is equipped with test paper strip socket and sample inlet, and every test paper strip socket's position corresponds with every test paper strip, the sample inlet pass through switching mechanism with the liquid reserve tank is linked together.
Further, the switching mechanism comprises a sleeve fixedly arranged at the inner bottom of the liquid storage tank and a guide rod movably inserted into the sleeve; the sleeve is provided with a plurality of groups of first through holes which are arrayed and distributed and penetrate through the sleeve, and the guide rod is provided with a plurality of groups of second through holes which are arrayed and distributed and correspond to the first through holes; the guide rod is provided with a first position and a second position relative to the sleeve, when the guide rod is positioned at the first position, the first through hole and the second through hole are mutually aligned and communicated, and a liquid channel is formed so that the liquid storage tank is communicated with each detection channel of the reaction cavity; when the guide rod is positioned at the second position, the first through hole and the second through hole are staggered and the first through hole is closed, so that each detection channel of the liquid storage tank and the reaction cavity is blocked.
Further, the guide rod is rotatably inserted in the sleeve, the first through hole and the second through hole are aligned and communicated or staggered when the guide rod rotates relative to the sleeve, the switching driving device is a rotating assembly, the rotating assembly is connected with one side of the guide rod, and the guide rod can rotate in the sleeve under the driving of the rotating assembly, so that the guide rod is switched between the first position and the second position.
Further, a spring is arranged in the sleeve, one end of the spring is fixed with one side of the guide rod, the other end of the spring is fixed with one side of the sleeve, and the spring drives the guide rod to slide along the axial direction of the sleeve when compressed or reset, so that the first through hole and the second through hole are aligned and communicated with each other or staggered with each other; the switching driving device comprises a first push rod used for pushing the guide rod and a first push rod driving mechanism used for driving the first push rod to move, and the first push rod driving mechanism is electrically connected with the control device.
Further, the test strip pushing device comprises a second push rod for pushing the shell and a second push rod driving mechanism for driving the second push rod to move, and the second push rod driving mechanism is electrically connected with the control device.
Further, the image acquisition device comprises a camera and an LED lamp.
Further, an incubation device is included, the chromatography device is disposed within the incubation device, and the incubation device is electrically connected to the control device.
Further, a stop device is included for limiting the end position of the housing movement.
Further, L-shaped baffles extending towards the outer side of the liquid storage tank are arranged on two sides of the liquid storage tank, a sliding groove is formed between the L-shaped baffles and the liquid storage tank, and a shell of the detection mechanism is slidably arranged in the sliding groove; the L-shaped baffle is provided with a limiting groove, the shell of the detection structure is provided with a buckle, and the limiting groove is connected with the buckle to limit the position of the test strip in the shell.
Further, a sealing piece for sealing the opening of the reaction cavity is arranged at the top of the reaction cavity; the sealing piece comprises a sealing film for sealing the test strip socket, and a puncturing device for puncturing the sealing film is arranged at the lower end of the shell of the detection mechanism.
Further, be equipped with two piece at least holding tanks in the casing, holding tanks parallel arrangement each other, the test paper strip is arranged in corresponding holding tank, the sample pad of test paper strip stretches out outside the casing and forms the extension, be provided with the observation window that is used for observing test paper strip testing result on the casing.
Further, the test strip is an immunochromatography test strip, the immunochromatography test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, and a detection line and a quality control line corresponding to a to-be-detected object are arranged on the chromatographic membrane; an antibody marker of an object to be detected or an antigen marker of the object to be detected is fixed in a detection channel of the reaction cavity.
Further, the antibody label of the object to be detected is a block of an antibody-label conjugate of the object to be detected; the antigen marker of the object to be detected is a block of an antigen-carrier protein-marker conjugate of the object to be detected; the detection line is coated with an antibody of an object to be detected or an antigen-carrier protein conjugate of the object to be detected, and the quality control line is coated with a goat anti-rabbit IgG antibody or a goat anti-mouse IgG antibody; the marker is colloidal gold.
Further, the number of the test strip sockets, the sample inlet, the first through holes, the second through holes and the test strips is the same and is 4-8.
The invention has the following beneficial effects:
according to the invention, the code scanning device is arranged to read the identification code on the detection mechanism, the control device identifies the test strip information according to the identification code, and specific working parameters of the test strip operation are extracted from the database, so that the chromatography detection equipment finishes a series of automatic control detection processes of releasing sample liquid from the liquid storage tank to the reaction cavity, shaking and incubation of the chromatography device, inserting the test strip into the reaction cavity in the chromatography device, reading the detection result and the like according to the information of the test strip, and on-site rapid detection personnel do not need to respectively memorize different time points for a plurality of different projects and different samples to operate, thereby greatly reducing error rate, improving real-time performance and accuracy of the result and meeting the intelligent experience of one-key operation. Meanwhile, a plurality of test strips can be read at the same time, and the result accuracy is better.
The equipment provided by the invention can realize automatic sample feeding, automatic shaking and automatic detection, and the whole process does not need manual operation, so that the working efficiency is greatly improved.
The detection equipment provided by the invention has the advantages of simple structure, compact arrangement of all devices, small occupied space, convenience in carrying, contribution to realization of miniaturization of the equipment, and high popularization and application values.
According to the invention, the switching mechanism is arranged between the liquid storage tank and the reaction tank and is used for controlling the liquid storage tank to be communicated with or cut off from each detection channel of the reaction cavity, and the reaction cavity comprises at least two mutually independent detection channels, so that sample liquid in the liquid storage tank can be synchronously and uniformly distributed into each detection channel in the reaction cavity, the average distribution of sample adding quantity is realized, the sample adding accuracy is improved, and the sample liquid in each detection channel can be regulated at any time according to the requirement, and the switching mode is reliable and convenient to operate; the independent reaction of chromatography in each detection channel can meet the requirement of environmental sensitivity type test, can avoid cross infection and ensure the accuracy of detection results; the detection of multiple target detection objects can be realized by one sample adding, the problems of uneven distribution and difficult control of distribution of sample liquid in combined detection are solved, multiple detection can be simultaneously and stably carried out, the high-flux detection is realized, the workload of detection personnel is effectively reduced, and the detection efficiency is improved.
In the invention, the detection mechanism is in sliding connection with the liquid storage tank, and the detection mechanism moves up and down along the outer wall of the liquid storage tank, so that a sample pad of the test strip is contacted with or separated from sample liquid flowing into each detection channel of the reaction cavity. Through the arrangement, the detection of the test strip by the two-step method can be realized, and if the sample liquid in the liquid storage tank needs to be uniformly mixed, the liquid storage tank can be manually shaken, the test strip cannot be polluted, the interference to adjacent reaction holes cannot be caused, and pipetting equipment such as a pipetting gun is not needed.
The detection by the chromatographic device is carried out in a relatively closed system, so that the sample pollution and the possibility of detecting pollution are reduced to a certain extent, the detection stability is further improved, and the hidden danger of manual operation errors is also reduced.
According to the invention, test strips for detecting different substances can be selected to be combined with the reaction tank according to actual detection requirements, so that different requirements are met.
In the invention, the test strip is arranged in the shell, so that the test strip is not easy to receive pollution (gloves or hands) in the operation process of an operator, and the operation is more convenient.
Drawings
FIG. 1 is a block diagram of the present invention;
FIG. 2 is a block diagram of a chromatographic apparatus of the invention;
FIG. 3 is a perspective view of the switching mechanism of the present invention;
fig. 4 is another perspective view of the switching mechanism of the present invention.
In the figure: 1. the chromatographic device, 2, test paper pushing device, 3, switching drive device, 4, oscillation device, 5, image acquisition device, 6, liquid reserve tank, 7, reaction chamber, 8, sleeve, 9, guide arm, 10, first through-hole, 11, second through-hole, 12, detection mechanism, 13, sample inlet, 14, test paper socket, 15, observation window, 16, L shape baffle, 17, spacing groove, 18, buckle, 19, spring.
Detailed Description
The utility model provides a high flux automatic chromatography check out test set, includes chromatographic device, test paper strip pusher, switching drive arrangement, concussion device, sweeps a yard device, image acquisition device and controlling means, test paper strip pusher, sweep a yard device, image acquisition device, switching drive arrangement, concussion device respectively with controlling means electricity is connected.
The chromatographic device comprises a liquid storage tank, a reaction cavity, a switching mechanism and a detection mechanism.
The liquid storage tank is used for containing sample liquid. The top of the liquid storage tank is provided with a liquid injection port, and the liquid injection port is sealed by a cover body.
The reaction cavity is arranged below the liquid storage tank and comprises at least two mutually independent detection channels.
The number of the detection channels is not particularly limited, and can be flexibly set according to the actual detection requirement by a person skilled in the art, and preferably, the number of the detection channels can be 2-8, for example, 6, 7 and 8.
Specifically, a longitudinal baffle plate is arranged in the reaction cavity, and the inner cavity of the reaction cavity is divided into a plurality of mutually independent detection channels by the longitudinal baffle plate; the top of reaction chamber corresponds every detection channel's position all is equipped with test paper strip socket and sample inlet, and every test paper strip socket's position corresponds with every test paper strip, the sample inlet pass through switching mechanism with the liquid reserve tank is linked together.
The reaction cavity is detachably connected with the liquid storage tank. The specific manner of removable connection is not particularly limited and may be accomplished in a manner conventional in the art and well known to those skilled in the art.
The switching mechanism is arranged between the liquid storage tank and the reaction cavity and used for controlling the liquid storage tank to be communicated with or cut off from each detection channel of the reaction cavity.
The switching mechanism comprises a sleeve fixedly arranged at the inner bottom of the liquid storage tank and a guide rod movably inserted into the sleeve; the sleeve is provided with a plurality of groups of first through holes which are arrayed and distributed and penetrate through the sleeve, and the guide rod is provided with a plurality of groups of second through holes which are arrayed and distributed and correspond to the first through holes; the guide rod is provided with a first position and a second position relative to the sleeve, when the guide rod is positioned at the first position, the first through hole and the second through hole are mutually aligned and communicated, and a liquid channel is formed so that the liquid storage tank is communicated with each detection channel of the reaction cavity; when the guide rod is positioned at the second position, the first through hole and the second through hole are staggered and the first through hole is closed, so that each detection channel of the liquid storage tank and the reaction cavity is blocked.
The first through holes are arrayed along the length direction of the sleeve, and the second through holes are arrayed along the length direction of the guide rod.
The present invention is not particularly limited to the arrangement of the first through holes and the second through holes, and the first through holes may be arranged in one row or two rows, for example.
Preferably, referring to fig. 3, the guide rod is rotatably inserted in the sleeve, and when the guide rod rotates relative to the sleeve, the first through hole and the second through hole are aligned and communicated with each other or are staggered from each other.
More preferably, referring to fig. 4, a spring is disposed in the sleeve, one end of the spring is fixed to one side of the guide rod, and the other end of the spring is fixed to one side of the sleeve, and when the spring is compressed or restored, the spring drives the guide rod to slide along the axial direction of the sleeve, so that the first through hole and the second through hole are aligned and communicated with each other or are staggered with each other.
The number of the groups of the first through holes and the second through holes is the same, and the number of the groups of the first through holes and the second through holes is determined according to the number of detection channels in the reaction cavity.
The position of each sample inlet corresponds to the first through hole. It is understood that the bottom of the liquid storage tank is the bottom of the sleeve, and the sample liquid placed in the liquid storage tank enters the detection channel through the relative movement of the sleeve and the guide rod.
According to the invention, the switching mechanism is adopted, so that the sample liquid in the liquid storage tank can be synchronously and uniformly distributed in each detection channel in the reaction cavity, the average distribution of the sample adding quantity is realized, the sample adding accuracy is improved, the sample liquid in each detection channel can be adjusted at any time as required, and the switching mode is reliable and the operation is convenient.
The detection mechanism comprises a shell and a plurality of test strips which are arranged in the shell and are parallel to each other. In the present invention, the manner of placement between the housing and the test strip is not particularly limited, as long as the test strip is easily fixed and removed from the housing, and can be set by those skilled in the art according to actual needs. Preferably, at least two holding grooves are arranged in the shell, the holding grooves are arranged in parallel, the test strip is arranged in the corresponding holding groove, and the sample pad of the test strip extends out of the shell to form an extension part.
The shell is provided with an observation window for observing the detection result of the test strip. In order to improve the airtight performance, a transparent plate is embedded in the observation window.
The housing may also be transparent, in which case no additional viewing window is required.
In the invention, the number of the test strips in the shell is not particularly limited, and a person skilled in the art can flexibly set the test strips according to the actual detection requirement, and preferably, the number of the test strips can be 4-8, for example, 6, 7 and 8.
The detection mechanism is arranged above the reaction cavity and is in sliding connection with the liquid storage tank, and the detection mechanism moves up and down along the outer wall of the liquid storage tank, so that a sample pad of the test strip is contacted with or separated from sample liquid flowing into each detection channel of the reaction cavity.
The invention further comprises a limiting device for limiting the end position of the movement of the shell. Through setting up stop device for test strip in the casing contacts with the sample liquid in the detection channel and takes place immunoreaction, the casing is fixed. Through this kind of setting for the test strip in the casing can not excessively push into the detection passageway of reaction chamber in lead to the test strip rupture, also can not make the casing upwards remove when detecting so that the test strip in the casing and the sample liquid separation in the detection passageway lead to immune reaction to stop.
The limiting device is preferably of a clamping groove structure. Specifically, two sides of the liquid storage tank are respectively provided with an L-shaped baffle extending towards the outer side direction of the liquid storage tank, a chute is formed between the L-shaped baffle and the liquid storage tank, and a shell of the detection mechanism is slidably arranged in the chute; the L-shaped baffle is provided with a limit groove, the shell of the detection structure is provided with a buckle, and the limit groove is connected with the buckle to limit the position of the test strip, so that the shell is limited to slide continuously towards the reaction cavity.
In the invention, the top of the reaction cavity is provided with a sealing piece for sealing the opening of the reaction cavity, and the sealing piece is used for sealing the space inside the reaction cavity from being in contact with the outside.
The sealing piece comprises a sealing film for sealing the test strip socket, and a puncturing device for puncturing the sealing film is arranged at the lower end of the shell of the detection mechanism.
It will be appreciated that the lancing device extends from the lower end of the housing to form a projection which extends out of the housing.
In the present invention, the sealing film is selected from a penetrable film material having a certain sealing property against the preservation solution and being easily penetrable by a sharp substance, typically but not limited to, such as tin foil paper or aluminum foil paper.
In the present invention, the lancing device is not particularly limited as long as it is ensured that the sealing film can be lanced when the test strip is inserted into the test strip insertion port. As a preferred embodiment, the port of the lancing device is serrated to enable lancing of the sealing membrane. As another preferred embodiment, the lancing device comprises a plurality of lancing members capable of lancing the sealing film, the lancing members preferably being spikes.
According to the invention, the test strip socket is sealed in a sealing mode easy to puncture, and when the detection mechanism shell slides downwards, the puncture device arranged at the lower end of the shell punctures the sealing film, so that the markers pre-fixed in each detection channel in the reaction cavity can be better stored, and the accuracy of the detection result is ensured.
The sealing piece is arranged outside the sample inlet in a covering way, and can be taken away firstly during detection and then the reaction cavity is connected to the lower part of the liquid storage tank.
The test strip pushing device is arranged above the chromatographic device and is used for driving the detection mechanism to slide along the outer wall of the liquid storage tank, so that a sample pad of the test strip is contacted with sample liquid flowing into each detection channel of the reaction cavity.
In the invention, the test strip pushing device comprises a second push rod for pushing the shell and a second push rod driving mechanism for driving the second push rod to move, and the second push rod driving mechanism is electrically connected with the control device. Wherein the second pushrod driving mechanism includes, but is not limited to, a solenoid valve, a stepper motor, and a servo motor. It will be appreciated that the test strip pushing device includes, but is not limited to, the above-mentioned structure, and may be other structures not shown in the present embodiment but well known to those skilled in the art, so long as the effect of driving the detection mechanism to slide along the outer wall of the liquid storage tank is achieved.
The switching driving device is arranged on one side of the chromatographic device and is used for driving the switching mechanism to move so that the liquid storage tank is communicated with or cut off from each detection channel of the reaction cavity.
The structure of the switching driving device is set according to the structure of the switching mechanism, and the effect of driving the switching mechanism to move so that the liquid storage tank is communicated with or cut off from each detection channel of the reaction cavity can be achieved.
Specifically, in the switching mechanism, the guide rod is rotatably inserted in the sleeve, when the guide rod rotates relative to the sleeve, the first through hole and the second through hole are aligned and communicated with each other or are staggered with each other, the switching driving device is a rotating assembly, the rotating assembly is connected with one side of the guide rod, and the guide rod can rotate in the sleeve under the driving of the rotating assembly, so that the guide rod is switched between the first position and the second position.
In the switching mechanism, be provided with the spring in the sleeve, the one end of spring with guide arm one side is fixed, the other end with sleeve one side is fixed, and the spring is compressed or drive during reset the guide arm slides along sleeve axis direction, makes when first through-hole and second through-hole align each other and communicate or stagger each other, the switching drive arrangement is including being used for promoting the first push rod of guide arm, be used for the drive first push rod actuating mechanism that first push rod removed, first push rod actuating mechanism with controlling means electricity is connected. Wherein the first pushrod driving mechanism includes, but is not limited to, a solenoid valve, a stepper motor, and a servo motor.
The vibration device is connected with the chromatography device and is used for driving the chromatography device to vibrate.
According to the invention, by arranging the oscillation device, the reagent fixed in the reaction cavity and the sample liquid flowing into the reaction cavity can be uniformly mixed, the detection sensitivity is high, the consistency is good, and the detection effect is good.
In the present invention, the structure of the oscillation device is not particularly limited, and any structure known in the art can be adopted by a person skilled in the art as long as the reagent fixed in the reaction chamber and the sample liquid flowing into the reaction chamber are uniformly mixed. Preferably, the chromatographic device is arranged on the oscillation mechanism and used for driving the chromatographic device to vibrate. The oscillating mechanism is a general standard component or a component known by a person skilled in the art, the structure and the principle of the oscillating mechanism are known by the person skilled in the art through a technical manual or by a conventional experimental method, the specific structure of the oscillating mechanism is not limited, and the person skilled in the art can adopt the existing structure. By way of example, the oscillation mechanism comprises an oscillation tray, the chromatographic device is arranged on the oscillation tray, an oscillation motor is connected below the oscillation tray, and the oscillation motor is electrically connected with the control device.
It should be noted that the motion track of the oscillation mechanism in the invention may be a straight line or a circle, and the oscillation mechanism enables the chromatographic device arranged on the oscillation mechanism to oscillate up and down, oscillate left and right, oscillate back and forth or oscillate around.
The code scanning device is used for collecting information of identification codes arranged on the detection mechanism.
In the present invention, the detection mechanism is provided with an identification code, and the identification code may be a one-dimensional code or a two-dimensional code, but is not limited thereto.
It can be understood that in the present invention, a plurality of test strips disposed in the housing share one identification code.
The code scanning device comprises a bracket and a scanning probe arranged on the bracket, and the scanning probe is a one-dimensional or two-dimensional scanner.
In the invention, the test paper strip is provided with an identification code, the code scanning device is used for reading the identification code on the detection structure and communicating with the control device to enable the control device to identify the information of the test paper strip so as to retrieve relevant processing control parameters from a product code database, such as the time of immune reaction of a plurality of test paper strips arranged on the shell, the movement time of the vibration device, the incubation time, the temperature and the like.
According to the invention, the code scanning device is arranged to read the identification code of the test strip, the control device identifies the information of the test strip according to the identification code, and specific working parameters of the test strip operation are extracted from the database, so that the chromatography detection equipment completes a series of automatic control detection processes according to the information of the test strip, and the detection process is completely intelligent.
The image acquisition device is used for acquiring an image of a color development area on the test strip.
The image acquisition device disclosed by the invention can adopt a plurality of known structures which are commonly used for acquiring and storing images and are disclosed by the prior art, and comprises a camera and an LED lamp. When the mobile terminal works, the detection equipment lights the LED lamp, starts the camera to photograph the test strip, transmits the test strip to the control device for processing, and uploads the processing result to the external mobile terminal. The camera generally comprises a lens and an image acquisition chip, and the lens and the image acquisition chip are matched with each other and are commonly used for acquiring images of a color development area on the test strip; more preferably, the image acquisition chip is a CCD image sensor. The LED lamp can be utilized for supplementing light to provide sufficient light sources for image recognition of the camera so as to improve the image recognition effect.
In the invention, the number of cameras and LED lamps is not particularly limited, and preferably, the number of cameras may be 1-8, more preferably 2-6, and the number of LED lamps may be 1-16.
The control device controls the test strip pushing device, the code scanning device, the image acquisition device, the switching driving device and the oscillation device to operate. The control device is a component known by a person skilled in the art, the structure and the principle of the control device are known by the person skilled in the art through technical manuals or are known by routine experimental methods, the person skilled in the art can set the control device according to actual requirements, and the specific structure of the control device is not limited.
The chromatographic detection device is used for immunodetection, and the test strip is an immunochromatographic test strip.
The chromatographic detection provided by the invention can be used for one-step immunochromatography and two-step immunochromatography when being used for immunological detection.
When the chromatographic detection is used for immunochromatography of a one-step method, the test strip comprises a bottom plate, a sample pad, a combination pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, and an antibody marker of an object to be detected or an antigen marker of the object to be detected is fixed on the combination pad; and the chromatographic membrane is provided with a detection line and a quality control line which correspond to the to-be-detected object.
When the chromatographic detection is used for immunochromatography by a two-step method, the immunochromatography test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, and a detection line and a quality control line corresponding to a to-be-detected are arranged on the chromatographic membrane. Specifically, the immunochromatographic test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad, wherein the sample pad is arranged on one side of the bottom plate, the water absorption pad is arranged on the other side of the bottom plate, the chromatographic membrane is arranged between the sample pad and the water absorption pad, the adjacent sample pad and the chromatographic membrane are in overlapped connection at the joint, the chromatographic membrane and the water absorption pad are in overlapped connection at the joint, a detection line and a quality control line are arranged in the middle of the chromatographic membrane, the detection line is close to the sample pad, and the sample pad of the test strip extends out of a shell to form an extension part; an antibody marker of an object to be detected or an antigen marker of the object to be detected is fixed in a detection channel of the reaction cavity.
The two-step immunochromatography sets the label conjugate coated in the existing binding pad in each detection channel of the reaction cavity, so that the label conjugate can react with the sample liquid to be detected more fully, the defects of incomplete reaction, low efficiency and the like on the binding pad in the traditional immunochromatography test strip are overcome, the label conjugate and the analyte to be detected can react thoroughly in a homogeneous system, and the sensitivity is improved.
The antibody label of the object to be detected is a block of an antibody-label conjugate of the object to be detected; the antigen marker of the object to be detected is a block of an antigen-carrier protein-marker conjugate of the object to be detected; the detection line is coated with an antibody of an object to be detected or an antigen-carrier protein conjugate of the object to be detected, and the quality control line is coated with a goat anti-rabbit IgG antibody or a goat anti-mouse IgG antibody; the label is a substance that can be used to identify and detect the collected signal, such as, but not limited to, colloidal gold.
The number of the test strip sockets, the sample inlet, the first through holes, the second through holes and the test strips is the same and is 4-8.
As a further improvement, an incubation device is included, the chromatography device being disposed within the incubation device, the incubation device being electrically connected to the control device. In the invention, by arranging the incubation device, a proper incubation temperature is provided for the test strip, the molecular movement speed is improved, reactants are promoted to be fully contacted, the time for the immune reaction to reach equilibrium is shortened, and the enough high sensitivity is ensured.
The temperature of the incubation device ranges from 4 ℃ to 120 ℃.
The incubation device may take the form of a structure known in the art, comprising, by way of example, an incubation well and a temperature control electronics module located at the bottom surface of the incubation well for controlling the temperature of the incubation well.
Taking the two-step method for immune detection as an example, the detection method of the invention comprises the following steps: collecting a sample, processing the sample to obtain a sample liquid, placing the sample liquid into a liquid storage tank, and placing a chromatographic device on the oscillating device; the code scanning device reads the identification code set on the test paper strip and transmits the identification code to the control device; the control device controls the switching driving device to work, so that the switching mechanism moves to enable the liquid storage tank to be communicated with each detection channel of the reaction cavity, sample liquid in the liquid storage tank flows into each detection channel through a sample inlet on the reaction cavity, and after preset time, the switching driving device moves to drive the switching mechanism to move to enable each detection channel of the liquid storage tank and the reaction cavity to be cut off; according to the temperature requirement of each test strip, the test strips are incubated by an incubation device; the control device controls the vibration device to work, so that an object to be detected in the sample liquid is fully and uniformly mixed with an antibody marker of the object to be detected or an antigen marker of the object to be detected fixed in the detection channel to react, after a preset reaction time, the control device controls the test strip pushing device to work, the assembled detection mechanism slides downwards, a puncture device arranged at the lower end of a shell in the detection mechanism punctures a sealing film, the test strip is inserted into a test strip jack on the reaction cavity, a sample pad of the test strip contacts with the sample liquid in the detection channel to perform immunochromatography reaction, after a preset chromatographic reaction is performed for a preset time, the image acquisition device works, color band information presented on the test strip is acquired, and a result is fed back to the control device.
The present invention will be described in detail with reference to the following examples, which are only preferred embodiments of the present invention and are not limiting thereof.
Example 1
The utility model provides a high flux automatic chromatography check out test set, includes chromatographic device, test paper strip pusher, switching drive arrangement, concussion device, sweeps a yard device, image acquisition device and controlling means, test paper strip pusher, sweep a yard device, image acquisition device, switching drive arrangement, concussion device respectively with controlling means electricity is connected.
The chromatographic device comprises a liquid storage tank, a reaction cavity, a switching mechanism, a detection mechanism, a limiting device and a sealing piece; the liquid storage tank is used for containing sample liquid; the top of the liquid storage tank is provided with a liquid injection port, and the liquid injection port is sealed by a cover body; the reaction cavity is arranged below the liquid storage tank, a longitudinal baffle plate is arranged in the reaction cavity, and the inner cavity of the reaction cavity is divided into 6 mutually independent detection channels by the longitudinal baffle plate; the top of the reaction cavity is provided with a test strip socket and a sample inlet corresponding to each detection channel, the position of each test strip socket corresponds to each test strip, and the sample inlet is communicated with the liquid storage tank through a switching mechanism; the switching mechanism is arranged between the liquid storage tank and the reaction cavity and used for controlling the liquid storage tank to be communicated with or cut off from each detection channel of the reaction cavity; the switching mechanism comprises a sleeve fixedly arranged at the inner bottom of the liquid storage tank and a guide rod rotatably inserted into the sleeve; the sleeve is provided with 6 groups of first through holes which are arrayed and distributed through the sleeve, and the guide rod is provided with 6 groups of second through holes which are arrayed and distributed corresponding to the first through holes; when the guide rod rotates, the guide rod is provided with a first position and a second position relative to the sleeve, when the guide rod is positioned at the first position, the first through hole and the second through hole are mutually aligned and communicated, and a liquid channel is formed so that the liquid storage tank is communicated with each detection channel of the reaction cavity; when the guide rod is positioned at the second position, the first through hole and the second through hole are staggered and the first through hole is closed, so that each detection channel of the liquid storage tank and the reaction cavity is blocked; the detection mechanism comprises a shell, 6 accommodating grooves are formed in the shell and are arranged in parallel, the test strips are arranged in the corresponding accommodating grooves, a sample pad of each test strip extends out of the shell to form an extending part, and an observation window for observing the detection result of each test strip is formed in the shell; the detection mechanism is arranged above the reaction cavity, is in sliding connection with the liquid storage tank, and moves up and down along the outer wall of the liquid storage tank, so that a sample pad of the test strip is contacted with or separated from sample liquid flowing into each detection channel of the reaction cavity; the test strip is an immunochromatography test strip, the immunochromatography test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, and a detection line and a quality control line corresponding to a to-be-detected object are arranged on the chromatographic membrane; an antibody marker of an object to be detected or an antigen marker of the object to be detected is fixed in a detection channel of the reaction cavity; the antibody label of the object to be detected is a block of an antibody-label conjugate of the object to be detected; the antigen marker of the object to be detected is a block of an antigen-carrier protein-marker conjugate of the object to be detected; the detection line is coated with an antibody of an object to be detected or an antigen-carrier protein conjugate of the object to be detected, and the quality control line is coated with a goat anti-rabbit IgG antibody or a goat anti-mouse IgG antibody; the marker is colloidal gold; the limiting device is used for limiting the position of the shell; the two sides of the liquid storage tank are respectively provided with an L-shaped baffle extending towards the outer side direction of the liquid storage tank, a chute is formed between the L-shaped baffle and the liquid storage tank, and a shell of the detection mechanism is slidably arranged in the chute; the L-shaped baffle is provided with a limit groove, the shell of the detection structure is provided with a buckle, and the limit groove is connected with the buckle to limit the position of the test strip in the shell; the sealing piece is arranged at the top of the reaction cavity and is used for sealing the opening of the reaction cavity; the sealing piece comprises a sealing film for sealing the test strip socket, and a puncturing device for puncturing the sealing film is arranged at the lower end of the shell of the detection mechanism; the lancing device includes a plurality of lancing members, the lancing members being spikes.
The test strip pushing device is arranged above the chromatographic device and is used for driving the detection mechanism to slide along the outer wall of the liquid storage tank, so that a sample pad of the test strip is contacted with sample liquid flowing into each detection channel of the reaction cavity.
The switching driving device is arranged on one side of the chromatographic device and is used for driving the switching mechanism to move so that the liquid storage tank is communicated with or cut off from each detection channel of the reaction cavity; the switching driving device is a rotating assembly, the rotating assembly is connected with one side of the guide rod, and the guide rod can rotate in the sleeve under the driving of the rotating assembly, so that the guide rod is switched between the first position and the second position.
The vibration device is connected with the chromatography device and is used for driving the chromatography device to vibrate.
The code scanning device is used for collecting information of identification codes arranged on the detection mechanism.
The image acquisition device is used for acquiring an image of a color development area on the test strip. The image acquisition device comprises a camera and an LED lamp.
Example 2
The utility model provides a high flux automatic chromatography check out test set, includes chromatography device, test paper strip pusher, switching drive arrangement, concussion device, sweeps a yard device, image acquisition device, incubation device and controlling means, test paper strip pusher, sweep a yard device, image acquisition device, switching drive arrangement, concussion device, incubation device respectively with controlling means electricity is connected.
The chromatographic device comprises a liquid storage tank, a reaction cavity, a switching mechanism, a detection mechanism, a limiting device and a sealing piece; the liquid storage tank is used for containing sample liquid; the top of the liquid storage tank is provided with a liquid injection port, and the liquid injection port is sealed by a cover body; the reaction cavity is arranged below the liquid storage tank, a longitudinal baffle plate is arranged in the reaction cavity, and the inner cavity of the reaction cavity is divided into 8 mutually independent detection channels by the longitudinal baffle plate; the top of the reaction cavity is provided with a test strip socket and a sample inlet corresponding to each detection channel, the position of each test strip socket corresponds to each test strip, and the sample inlet is communicated with the liquid storage tank through a switching mechanism; the switching mechanism is arranged between the liquid storage tank and the reaction cavity and used for controlling the liquid storage tank to be communicated with or cut off from each detection channel of the reaction cavity; the switching mechanism comprises a sleeve fixedly arranged at the inner bottom of the liquid storage tank and a guide rod movably inserted into the sleeve; the sleeve is provided with 8 groups of first through holes which are arrayed and distributed and penetrate through the sleeve, and the guide rod is provided with 8 groups of second through holes which are arrayed and distributed and correspond to the first through holes; the device comprises a sleeve, a guide rod, a liquid storage tank, a reaction cavity, a first through hole, a second through hole, a spring, a first guide rod, a second guide rod, a first guide rod and a second guide rod, wherein the spring is arranged in the sleeve, one end of the spring is fixed with one side of the guide rod, the other end of the spring is fixed with one side of the sleeve, and the spring drives the guide rod to slide along the axial direction of the sleeve when compressed or reset, so that the guide rod has a first position and a second position relative to the sleeve; when the guide rod is positioned at the second position, the first through hole and the second through hole are staggered and the first through hole is closed, so that each detection channel of the liquid storage tank and the reaction cavity is blocked; the detection mechanism comprises a shell, wherein 8 accommodating grooves are formed in the shell and are arranged in parallel, the test strips are arranged in the corresponding accommodating grooves, a sample pad of each test strip extends out of the shell to form an extending part, and an observation window for observing the detection result of each test strip is formed in the shell; the detection mechanism is arranged above the reaction cavity, is in sliding connection with the liquid storage tank, and moves up and down along the outer wall of the liquid storage tank, so that a sample pad of the test strip is contacted with or separated from sample liquid flowing into each detection channel of the reaction cavity; the test strip is an immunochromatography test strip, the immunochromatography test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, and a detection line and a quality control line corresponding to a to-be-detected object are arranged on the chromatographic membrane; an antibody marker of an object to be detected or an antigen marker of the object to be detected is fixed in a detection channel of the reaction cavity; the antibody label of the object to be detected is a block of an antibody-label conjugate of the object to be detected; the antigen marker of the object to be detected is a block of an antigen-carrier protein-marker conjugate of the object to be detected; the detection line is coated with an antibody of an object to be detected or an antigen-carrier protein conjugate of the object to be detected, and the quality control line is coated with a goat anti-rabbit IgG antibody or a goat anti-mouse IgG antibody; the marker is colloidal gold; the limiting device is used for limiting the position of the shell; the two sides of the liquid storage tank are respectively provided with an L-shaped baffle extending towards the outer side direction of the liquid storage tank, a chute is formed between the L-shaped baffle and the liquid storage tank, and a shell of the detection mechanism is slidably arranged in the chute; the L-shaped baffle is provided with a limit groove, the shell of the detection structure is provided with a buckle, and the limit groove is connected with the buckle to limit the position of the test strip in the shell; the sealing piece is arranged at the top of the reaction cavity and is used for sealing the opening of the reaction cavity; the sealing piece comprises a sealing film for sealing the test strip socket, and a puncturing device for puncturing the sealing film is arranged at the lower end of the shell of the detection mechanism; the lancing device includes a plurality of lancing members, the lancing members being spikes.
The test strip pushing device is arranged above the chromatographic device and is used for driving the detection mechanism to slide along the outer wall of the liquid storage tank, so that a sample pad of the test strip is contacted with sample liquid flowing into each detection channel of the reaction cavity.
The switching driving device is arranged on one side of the chromatographic device and is used for driving the switching mechanism to move so that the liquid storage tank is communicated with or cut off from each detection channel of the reaction cavity; the switching driving device comprises a first push rod used for pushing the guide rod and a first push rod driving mechanism used for driving the first push rod to move, and the first push rod driving mechanism is electrically connected with the control device.
The vibration device is connected with the chromatography device and is used for driving the chromatography device to vibrate.
The code scanning device is used for collecting information of identification codes arranged on the detection mechanism.
The image acquisition device is used for acquiring an image of a color development area on the test strip; the image acquisition device comprises a camera and an LED lamp.
The chromatographic device is disposed within the incubation device.
The above examples only show embodiments of the present invention, and the description thereof is more specific and detailed, but should not be construed as limiting the scope of the invention, but all technical solutions obtained by equivalent substitution or equivalent transformation shall fall within the scope of the invention.
Claims (6)
1. A high throughput automated chromatographic detection apparatus comprising:
the chromatographic device comprises a liquid storage tank, a reaction cavity, a switching mechanism and a detection mechanism; the liquid storage tank is used for containing sample liquid; the reaction cavity is arranged below the liquid storage tank and comprises at least two mutually independent detection channels; the switching mechanism is arranged between the liquid storage tank and the reaction cavity and used for controlling the liquid storage tank to be communicated with or cut off from each detection channel of the reaction cavity; the detection mechanism comprises a shell and a plurality of test strips which are arranged in the shell and are parallel to each other;
the detection mechanism is arranged above the reaction cavity and is in sliding connection with the liquid storage tank;
the test strip pushing device is arranged above the chromatographic device and is used for driving the detection mechanism to slide along the outer wall of the liquid storage tank so that a sample pad of the test strip contacts with sample liquid flowing into each detection channel of the reaction cavity;
the switching driving device is arranged on one side of the chromatographic device and used for driving the switching mechanism to move;
the vibration device is connected with the chromatographic device and is used for driving the chromatographic device to vibrate;
The code scanning device is used for collecting information of the identification codes arranged on the detection mechanism;
the image acquisition device is used for acquiring an image of the color development area on the test strip;
the test strip pushing device, the code scanning device, the image acquisition device, the switching driving device and the oscillating device are respectively and electrically connected with the control device;
a longitudinal baffle plate is arranged in the reaction cavity, and the inner cavity of the reaction cavity is divided into a plurality of mutually independent detection channels by the longitudinal baffle plate; the top of the reaction cavity is provided with a test strip socket and a sample inlet corresponding to each detection channel, the position of each test strip socket corresponds to each test strip, and the sample inlet is communicated with the liquid storage tank through the switching mechanism;
the switching mechanism comprises a sleeve fixedly arranged at the inner bottom of the liquid storage tank and a guide rod movably inserted into the sleeve; the sleeve is provided with a plurality of groups of first through holes which are arrayed and distributed and penetrate through the sleeve, and the guide rod is provided with a plurality of groups of second through holes which are arrayed and distributed and correspond to the first through holes; the guide rod is provided with a first position and a second position relative to the sleeve, when the guide rod is positioned at the first position, the first through hole and the second through hole are mutually aligned and communicated, and a liquid channel is formed so that the liquid storage tank is communicated with each detection channel of the reaction cavity; when the guide rod is positioned at the second position, the first through hole and the second through hole are staggered and the first through hole is closed, so that each detection channel of the liquid storage tank and the reaction cavity is blocked;
The guide rod is rotatably inserted into the sleeve, the first through hole and the second through hole are aligned and communicated or staggered when the guide rod rotates relative to the sleeve, the switching driving device is a rotating assembly, the rotating assembly is connected with one side of the guide rod, and the guide rod can rotate in the sleeve under the driving of the rotating assembly, so that the guide rod is switched between the first position and the second position;
a spring is arranged in the sleeve, one end of the spring is fixed with one side of the guide rod, the other end of the spring is fixed with one side of the sleeve, and the spring drives the guide rod to slide along the axial direction of the sleeve when being compressed or reset, so that the first through hole and the second through hole are aligned and communicated with each other or are staggered with each other; the switching driving device comprises a first push rod used for pushing the guide rod and a first push rod driving mechanism used for driving the first push rod to move, and the first push rod driving mechanism is electrically connected with the control device.
2. The high throughput automated chromatography detection apparatus of claim 1, wherein said test strip pushing device comprises a second push rod for pushing said housing, a second push rod driving mechanism for driving movement of said second push rod, said second push rod driving mechanism being electrically connected to said control device; the image acquisition device comprises a camera and an LED lamp.
3. The high throughput automated chromatography detection apparatus of claim 1, further comprising an incubation device disposed within said incubation device, said incubation device being electrically connected to said control device.
4. The high-throughput automatic chromatography detection apparatus according to claim 1, wherein both sides of the liquid storage tank are provided with L-shaped baffles extending towards the outer side direction of the liquid storage tank, a chute is formed between the L-shaped baffles and the liquid storage tank, and a shell of the detection mechanism is slidably arranged in the chute; the L-shaped baffle is provided with a limiting groove, the shell of the detection structure is provided with a buckle, and the limiting groove is connected with the buckle to limit the position of the test strip in the shell.
5. The high-throughput automated chromatography detection apparatus of claim 1, wherein a seal for sealing an opening of the reaction chamber is provided at a top of the reaction chamber; the sealing piece comprises a sealing film for sealing the test strip socket, and a puncturing device for puncturing the sealing film is arranged at the lower end of the shell of the detection mechanism.
6. The high-throughput automatic chromatographic detection device according to claim 1, wherein the test strip is an immunochromatographic test strip, the immunochromatographic test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to a chromatographic direction, and a detection line and a quality control line corresponding to a to-be-detected are arranged on the chromatographic membrane; an antibody marker of an object to be detected or an antigen marker of the object to be detected is fixed in a detection channel of the reaction cavity.
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| CN110967337A (en) * | 2019-12-02 | 2020-04-07 | 重庆凡聚智能科技有限公司 | Drug detection device based on sewer component detection |
| CN114563523B (en) * | 2022-04-29 | 2022-07-22 | 常州美洛医疗科技有限公司 | Thin layer chromatography jar |
| CN114965995B (en) * | 2022-05-20 | 2023-04-11 | 河南申友医学检验所有限公司 | Immunochromatography analysis detection system and method |
| CN115032404B (en) * | 2022-06-10 | 2023-05-23 | 武汉光谷联合医学检验所股份有限公司 | Vaccine antibody detection card, detection system and detection method thereof |
| CN117871853B (en) * | 2024-03-13 | 2024-05-24 | 南通戴尔诺斯生物科技有限公司 | Colloidal gold immunochromatography equipment for detecting biotoxin and application method thereof |
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