CN109146882A - A kind of food safety colloid gold test paper analytical equipment and its application - Google Patents

A kind of food safety colloid gold test paper analytical equipment and its application Download PDF

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CN109146882A
CN109146882A CN201811257592.XA CN201811257592A CN109146882A CN 109146882 A CN109146882 A CN 109146882A CN 201811257592 A CN201811257592 A CN 201811257592A CN 109146882 A CN109146882 A CN 109146882A
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data
colloidal gold
information
image
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杨春江
莫勋
袁志波
吴迪
宋世燕
邰宗洋
郑云鹏
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Beijing One Hundred Biological Technology Co Ltd
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Abstract

The present invention provides a kind of food safety colloid gold test paper analytical equipments, are made of data acquisition unit, data processing and computing unit, data outputting unit, data-printing unit.It is handled using intelligent data acquisition analysis, and is linked up with cloud data platform, finally realizes that food-safe detection data provides long-term preservation, can not change and carries out the function of early warning to particular risk.By multiple device network consisting early warning platforms, effectively it can be distributed in the inspection center of each region, testing laboratory by centralized management, and detection data is intuitive, can be traced, there is stronger practical application.

Description

A kind of food safety colloid gold test paper analytical equipment and its application
Technical field
The present invention relates to technical field of food safety detection, and in particular to a kind of food safety colloid gold test paper analytical equipment And its application.
Background technique
The focus that food safety (Food Safety) has become global concern, is related to animal derived in food safety Food such as meat, egg, milk etc. is even more the emphasis of food safety Regulation.The pollutant being related in food safety at present has agricultural and veterinary chemicals residual Stay, illegal additive, mycotoxin, heavy metal and pathogenic microorganisms etc., veterinary drug residue, mycotoxin harm more closely Come constantly known to masses over year.Detection and supervision for these hazardous substances have instrumental method such as high performance liquid chromatography, liquid Phase combined gas chromatography mass spectrometry, near-infrared analysis etc. also have rapid detection method such as enzyme linked immunosorbent detection technology, colloidal gold immunochromatographimethod Detection technique etc., these method characteristics are different, and application is different, and whole all food-safe horizontal improve plays positive work With.
In the most not full-time analytical test personnel, special reality such as base's production unit such as pasture, milking station, chicken farm Room and expensive testing equipment are tested, therefore it is carried out all kinds of detection and analysis and often uses the primary dcreening operations hand such as colloid gold immune test strip Section, and the requirement that more thick spacious, experimental data reservation is often unable to satisfy food safety traceability is operated, also it is unfavorable for Food safety collection neutralizes real-time monitoring.
Summary of the invention
In order to improve base's detection unit to testing result interpretation ability, the requirement for improving its preservation detection data, satisfaction Food safety traceability and the demand for concentrating real-time monitoring, the present invention provides a kind of analyses of food safety colloid gold test paper to fill It sets, is handled using intelligent data acquisition analysis, and linked up with cloud data platform, finally realize food-safe testing number According to the function of providing long-term preservation, can not change and carry out early warning to particular risk.
In order to achieve the above-mentioned object of the invention, food safety colloid gold test paper analytical equipment provided by the present invention, by following Unit is constituted:
1. data acquisition unit:
Using CCD camera, CMOS camera or the data acquisition equipment for having same function, colloidal gold strip is detected Image is acquired, and the image-region containing colloid gold test paper detection feature is transmitted to data processing and computing unit.
Further, above-mentioned data acquisition unit can be also digital picture scanning means, and glue is acquired by way of scanning Body gold test paper strip detection image or the image-region that feature is detected containing colloid gold test paper.
Further, the image-region containing colloid gold test paper detection feature mainly includes colloidal gold colloidal gold detection test paper strip Detection line, nature controlling line region.
Further, the image-region containing colloid gold test paper detection feature can also include that can identify or mark The characteristic information of detection project, such as the digital number information or bar code letter of the stickup in colloidal gold colloidal gold detection test paper strip handheld terminal Breath.
Further, in order to accurately obtain colloidal gold detection characteristic image region, the data acquisition unit can be with Adapter including being adapted to various forms of colloidal gold strips, such as be adapted to the colloidal gold strip of slip-type, contain plastics The colloidal-gold detecting-card to get stuck or the multi-joint strip etc. being made of several independent colloidal gold strips.
Further, in order to accurately obtain colloidal gold detection characteristic image region, the data acquisition unit can be with Including fixed LED or other respective sources, for stablizing image capture environment, it is ensured that the stability of testing result.
2. data processing and computing unit:
The data of data acquisition unit transmission are subjected to digital conversion, analyze its characteristic value, and in the way of characteristic value calculating Determine that the result of colloidal gold detection is recorded, and stores in the specific format.
Further, in order to colloidal gold colloidal gold detection test paper strip detection project is confirmed and is calibrated in advance, above-mentioned data Processing and computing unit are additionally provided with configuration data input module, wherein the data input module be can read type-C, The storage card or ID card of the devices such as USB read equipment, can read two dimensional code configuration information two-dimensional code scanning device, can be from The module of the network channels such as WLAN, GPRS, 4G/5G acquisition cloud configuration information.
Further, the configuration information of the read colloidal gold colloidal gold detection test paper strip of configuration data input module includes glue The prefabricated detection of the prefabricated curve of body gold test strip detection project, testing product lot number, testing product, testing product is sensitive Degree etc..
Further, the data processing and computing unit further include liquid crystal display, for showing data acquisition unit The colloidal gold detection characteristic image of input and the data information of notebook data processing and computing unit analysis, such as: contain colloidal gold The image-region for detecting feature and its characteristic value, colloidal gold testing result, sample information, operator after digital change process Member etc..
Further, the data processing and computing unit further include the physical button convenient for operation, for detection The auxiliary operation of process, the physical button include at least on & off switch, directionkeys, acknowledgement key.
3. data outputting unit:
Using the network channels such as WLAN, GPRS, 4G/5G by data processing and computing unit handle according to specific format storage Data are transmitted to cloud data platform, wherein the data transmitted include at least: detection device location information (IP address, GPS letter Breath etc.), detection time stamp, containing colloidal gold detection feature image-region and its characteristic value after digital change process, glue Body gold testing result, sample information, operator etc..
Further, the data of the data outputting unit transmission further include containing above-mentioned detection information and its independent characteristic Encrypted code, to ensure the uniqueness and unalterable feature of information, such as encryption contains above-mentioned detection device location information (IP address, GPS information etc.), detection time stamp detect the image-region of feature containing colloidal gold and its through digital change process Rear characteristic value, colloidal gold testing result, sample information, operator encrypted code.
Further, the output information of the data outputting unit transmission can also pass through every food safety colloidal gold The equipment unique information such as equipment Serial Number or mac address of nic of test paper analytical equipment is as encryption seed.
Further, data processing and computing unit can also include built-in SIM card module, complete in Detection task When with short message, perhaps other way notifies user or notifies user when there is serious positive reaction result.
4. data-printing unit:
It will test result using the printer that bluetooth or data line connect to print in the specific format, Locale Holding achieves.
It is interconnected between above-mentioned modules unit, interdepends and form food safety colloid gold test paper of the invention point Analysis apparatus.
The testing result of colloidal gold strip is tested and analyzed using food safety colloid gold test paper analytical equipment of the invention Concrete operation step include:
Step 1: device for opening switchs, testing product project and its configuration information, example are obtained using ID card, network server end Such as enter the data processing of the present apparatus and the USB interface of computing unit using ID card insertion, data processing and computing unit can obtain automatically Take wherein configuration information;Detection project configuration information directly is obtained from network server end using data processing and computing unit Deng;
The project that selection will test from acquired project configuration information, it is spare.
It is slightly handled step 2: will test the colloidal gold strip finished, such as removes extra liquid using blotting paper, It is placed into the colloidal gold strip adapter of data acquisition unit, such as is adapted to the colloidal gold strip of slip-type, containing modeling Expect the colloidal-gold detecting-card to get stuck or the multi-joint strip etc. being made of several independent colloidal gold strips, it is ensured that colloidal gold The results area of test strips can correctly be acquired by data acquisition unit.
Step 3: data acquisition unit is by the characteristic image of automatic collection colloidal gold strip testing result detected, And it is input to data processing and computing unit in a digital format.Data processing and computing unit receive colloidal gold testing result figure As first being pre-processed after information, image transformation to obtain its characteristic image, and further by numerical transformation removal image make an uproar Sound and background obtain more clean image information.Continue according to preset detection project configuration information to characteristics of image Value extracts, such as red (R) by obtaining picture signal, green (G), blue (B) information or tone (H), saturation degree (S) are bright (V) information is spent, the characteristic value of colloidal gold strip detection line T line and nature controlling line C line is calculated, and further calculates its ratio.? In the presence of having standard curve, it can use standard curve and quantitative calculating carried out to unknown sample.And finally by quantitative scoring The result of calculation exports.
Step 4: the testing result that data processing and computing unit processing are completed can be exported to the display screen of this unit On, it can also be permanently stored while display by data outputting unit network transmission to cloud platform.The information transmitted with It is transmitted after the sequence number or machine code encryption of device itself, the information transmitted includes containing above-mentioned detection device location information (IP address, GPS information etc.), detection time stamp detect the image-region of feature containing colloidal gold and its through digital change process Characteristic value, colloidal gold testing result, sample information, operator afterwards etc..
Compared with traditional technology and existing detection device, the beneficial effects of the present invention are:
Detection is efficient, result is accurate, strong with colloidal gold strip testing result consistency;As a result uniqueness, trackability are strong;Inspection Survey result is comprehensive, information is complete, facilitates the maintenance of food security information chain;Testing result feedback in real time, can be effectively reduced Or product of reducing risks enters the market, to reduce food safety risk, it is ensured that manage and close rule.Multiple devices can be formed effectively when being combined Food safety detection big data, food-safe Risk-warning has strong effect.
Detailed description of the invention
Fig. 1 one embodiment of the invention described device structural block diagram.
Fig. 1-1 one embodiment of the invention described device data acquisition unit structural block diagram.
Fig. 1-2 one embodiment of the invention described device data processing and computing unit structural block diagram.
Fig. 2 food safety colloid gold test paper analytical equipment analysis detection flow chart.
Fig. 3 one embodiment of the invention colloidal gold colloidal gold detection test paper strip characteristic image extracts and analysis flow chart diagram.
Fig. 3 one embodiment of the invention colloidal gold colloidal gold detection test paper strip characteristic image extracts and analysis flow chart diagram.
The colloidal gold strip testing result image of Fig. 3-1 one embodiment of the invention.
The colloidal gold strip testing result image characteristic region of Fig. 3-2 one embodiment of the invention.
The colloidal gold strip testing result image characteristic region numerical curve figure of Fig. 3-3 one embodiment of the invention.
Fig. 4 one embodiment of the invention data and its encryption conveying flow figure.
The food safety early warning system figure of Fig. 5 application apparatus of the present invention.
Further describe the present invention With reference to embodiment.The advantages and features of the present invention will be with describing And it is apparent, but these embodiments are only exemplary in nature, it is not intended to limit the scope of the present invention in any way.Art technology Personnel should be understood that without departing from the spirit and scope of the invention can details and form to technical solution of the present invention It modifies or replaces, but these modifications and replacement each fall within protection scope of the present invention.
One of structure of 1 apparatus of the present invention of embodiment is implemented
Food safety colloid gold test paper analytical equipment (100) includes data acquisition unit as shown in Fig. 1, Fig. 1-1 and Fig. 1-2 (200), data processing and computing unit (300), data outputting unit (400) and data print unit (500), in which:
Data acquisition unit (200) is mainly by image collecting device (201), colloidal gold strip adapter (202), fixed light source (203) it forms, wherein image collecting device (201) can be CCD camera, CMOS camera or the data for having same function Equipment is acquired, is also possible to digital picture scanning means, it is therefore an objective to acquire colloidal gold strip detection image or contain colloidal gold The image-region of test paper detection feature;Colloidal gold strip adapter (202) is to be adapted to various forms of colloid gold test papers Item, such as the colloidal gold strip of adaptation slip-type, the colloidal-gold detecting-card or independent by several that gets stuck containing plastics The multi-joint strip etc. of colloidal gold strip composition;Fixed light source (203) be LED light composition be fixed on image collecting device (201) light source of surrounding, for stablizing image capture environment, it is ensured that its stability acquired.
Data processing and computing unit (300) are calculating and the control centre of whole device, are responsible for image real time transfer point Analysis and the control of other each units, mainly include central processing module (301), display module (302), control module (303), memory module (304), power supply module (305), input module (306);Wherein central processing module (301) is core mould Block is responsible for data processing, calculating, control program etc.;Display module (302) is the control display device being made of touch screen, is used In manipulation food safety colloid gold test paper analytical equipment (100);The control that control module (303) is made of several control buttons Platform processed is used for auxiliary operation food safety colloid gold test paper analytical equipment (100);Memory module (304) is by flash memory hard disk group At memory, for storage apparatus device operating system, analysis program, the softwares relevant information such as detection project configuration information; The integration module that power supply module (305) is made of battery and power supply change-over device is used for battery when on-site test and powers, and electric With power supply line connection common power power supply when pond is fed;Input module (306) is read by the incoming device with input interface Project configuration information in USB flash memory, such as bulleted list, item argument, product batches information are used for food safety colloidal gold Test paper analytical equipment (100) operation.
Data outputting unit (400) is the module and its device for having network interface and efferentation, including WIFI, GPRS, LAN etc., wherein WIFI and LAN interface are the requisite interfaces of this system, on the one hand can be used for the outflow of detection data, Such as it is transmitted to cloud data platform, or be transmitted to computer system, it on the one hand can also be used as the control input of this system, example After such as being connect with computer system, this system is debugged and is arranged using host computer.
Print unit (500) is the direct print module of data being made of miniprinter, when data processing and is calculated single After first (300) have handled data, it can directly pass through print unit (500) printout, facilitate user's on-the-spot record and viewing.
A kind of workflow of 2 apparatus of the present invention of embodiment
As shown in Fig. 2, existing one of workflow with regard to apparatus of the present invention is described as follows:
(1) device for opening switchs, and the data processing of the present apparatus and the USB interface of computing unit, data processing are entered using ID card insertion And computing unit can obtain wherein configuration information such as testing product project and its configuration information automatically;Simultaneously from acquired project The project that selection will test in configuration information, it is spare.
(2) it will test the colloidal gold strip finished slightly to handle, such as remove extra liquid using blotting paper, place Enter in the colloidal gold strip adapter of data acquisition unit.
(3) data acquisition unit is by the characteristic image of automatic collection colloidal gold strip testing result detected, and with Number format is input to data processing and computing unit.Data processing and computing unit receive colloidal gold detection result image letter First pre-processed after breath, image transformation to obtain its characteristic image, and further by numerical transformation removal picture noise and Background obtains more clean image information.Continue according to preset detection project configuration information to image feature value into Row extracts the result for calculating, and finally calculating quantitative scoring and exports.
(4) testing result that data processing and computing unit processing are completed export it is same on display screen to this unit When, it will it is permanently stored by data outputting unit network transmission to cloud platform.The information transmitted is with the sequence of device itself Number encryption after transmit, including containing above-mentioned detection device location information (IP address, GPS information etc.), detection time stamp, contain There are the image-region of colloidal gold detection feature and its characteristic value after digital change process, colloidal gold testing result, sample letter Breath, operator etc..
3 one embodiment of the invention colloidal gold colloidal gold detection test paper strip characteristic image of embodiment extracts and analysis process flow
As shown in figure 3, existing describe such as with regard to the extraction of one embodiment of the invention colloidal gold colloidal gold detection test paper strip characteristic image and analysis process Under:
(1) characteristic area is searched: the colloidal gold strip testing result characteristic image acquired using data acquisition unit (200), It is handled through data processing and computing unit (300), such as through binary transform, searches boundary etc., determine colloidal gold strip The characteristic area of testing result.
(2) it cuts: continuing that the characteristic area of lookup is carried out to pinpoint to cut, it is ensured that the integrality and interference letter of detection information The minimum of breath.
(3) data analysis and transformation: progressively scanning or scan by column acquisition image pixel data matrix using image pixel, Characteristic therein is extracted, such as by analysis by ready image data matrix, extracts its R, G, in channel B A certain channel data perhaps the average value in three channels or extract the wherein data of its H, S, V data, utilize it Draw the one kind of pixel change curve as output information.
(4) it calculates characteristic value: utilizing various filter process pixel datas, reduce noise, improve signal-to-noise ratio, ensure simultaneously Signal is undistorted.The data handled well are further analyzed, calculating colloidal gold colloidal gold detection test paper strip T line, (including single T line is to more T line), C line (including single C line to more C lines) characteristic parameter, such as extreme value, the average value, peak area in T line region, C line area The peak value extreme value in domain, peak area, peak area etc..
(5) testing result is calculated:
The various characteristic parameters calculated in (4) are utilized, compare the characteristic parameter of T line Yu C line, such as calculate its character pair parameter Ratio marks as a result, compares with configuration information, determines testing result.
When in configuration information there are when standard curve information, can further using various regression analysis equations to result into Row processing, such as be fitted using cubic spline curve, 4 parameter logistic curves, 5 parameter logistic curves, even numbers curve etc..
4 one embodiment of the invention colloidal gold strip testing result feature value calculating method of embodiment
Now one embodiment of the invention colloidal gold strip testing result characteristics of image value calculating method is described as follows:
As shown in Fig. 3-1 and 3-2, wherein 1 is the colloidal gold strip testing result characteristic pattern of data acquisition unit (200) acquisition Picture, through data processing and computing unit (300) cut processing as a result, wherein the length of L representative image (is single with pixel Position), W representative image width (as unit of pixel), 2 represent C line, and 3 represent T line.
The size of W and L is obtained by data processing and computing unit (300), and is acquired according to 5% ~ 95% range of W value Image intermediate region (4 signified regions in such as Fig. 3-2), the purpose is to remove colloidal gold strip both sides image difference to knot Fruit influences.In order to ensure fetching according to representative, in the 10% ~ 90% of the width W of data area, such as 5% ~ 95% range of acquisition Image data or 10% ~ 80% range in image data.For convenience of calculation, the range of this pickup area is denoted as S, Then using image in computer program acquisition S regional scope along the pixel value of L cross direction profiles, such as its R, G, channel B are obtained Numerical value calculates its average value A(0 < A < 255), and filters filter noise is used, it obtains the channel plot that A is distributed along L and (such as schemes Shown in 3-3).
Since pixel color is compared with remaining region of test strips depth, obtained curve, which should be, to be had for C line region and T line region The curve of several troughs, and trough quantity is identical as the sum of T line number amount and C line number amount.By taking 1 C line and 1 T line as an example (such as Fig. 3-2 and 3-3) closes on difference acquisition C line and the T line coordinates position of numerical value by calculating, and wherein C line is denoted as (C1, C2), T line is denoted as (T1, T2), it is contemplated that C line and T line are dispersed on nitrocellulose filter due to liquid on colloidal gold strip, can be produced Raw lines two sides dizzy the phenomenon that opening, therefore the range of C, T line value can be further determined as to 5% ~ 95% region of current value Range discards remaining redundant data, such as: the width of C line and T line be 100 pixels, then C line characteristic value value range be 5px ~ Data within the scope of 95px, similarly also fetch bit can also further acquire 10px ~ 90px in data within the scope of 5px ~ 95px to T line Or the data within 5px ~ 90px.
Average value of C, T line in value range is successively calculated to be denoted as respectively………………………(4.1)
Or extreme value is denoted as Cmax、Tmax……………………………………………………………(4.2)
Or the area of the corresponding trough of C, T line in this area is denoted as Cs、Ts………………(4.3)
Final colloidal gold strip testing result can be calculated according to following formula:
Test=T/C, wherein T and C respectively represents the individual features value in 4.1,4.2 and 4.3.
By the Test value of calculating and preset threshold value comparison, that is, it can determine the positive and negative of final detection result.
The standard solution (S1, S2, S3, S4, S5, S6) for the gradient concentration that can also be prepared in advance, while utilizing colloidal gold Test strips detection, is denoted as T1, T2, T3, T4, T5, T6 for its corresponding testing result characteristic value.Then with four parameter logistic curves or The fitting of other response curve approximating methods, and fitting publicity is preset in ID card or is stored in network cloud.In new colloid Gold test paper strip, which is completed to calculate its characteristic value substitution curve equation after detecting, obtains quantitative detection result.
5 one embodiment of the invention data of embodiment and its encryption conveying flow
As shown in figure 4, an existing data just of the invention and its encryption conveying flow are described as follows:
The core component of data processing and computing unit (300) as apparatus of the present invention, in analysis processing image data and inspection Surveying after result calculates, data can be encrypted, and mainly include the following steps:
(1) result summarizes: will test time, detection project, detection result image, testing result data, testing staff, sample letter Breath is summarized, and wherein image is stored as JPEG or PNG in the compressed format, or with the storage of Base64 coding form, by this A little information combine in a suitable manner.
(2) add uniquely tagged: will test the device coding of device, such as sequence number or machine string information or MAC Address Etc. information generate unique encrypted characters string, as encrypted indicia.
(3) it encrypts: reversible encryption being carried out to the detection information summarized using above-mentioned encrypted indicia.
(4) it transmits: the registration information of each device is such as led to together with above-mentioned encryption in the account ID of cloud platform registration Data outputting unit (400) network transmission is crossed to cloud data platform, can be transmitted with single, it can also be with bulk transfer.
(5) platform decryption and record: platform receives after the information of transmission the decryption processing in a manner of queue and is recorded into cloud Database.
Embodiment 5 establishes food safety early warning system using apparatus of the present invention
As shown in figure 5, existing be just described using the food safety early warning system that apparatus of the present invention are established:
(1) apparatus of the present invention that will be distributed over different location are formed by internet (LAN, WIFI, GPRS, 4G, 5G etc.) connection Distributed Detection network with multiple intelligent terminals;
(2) using cloud data platform as data summarization analysis platform, the data that each terminal is passed to is summarized, are arranged And secondary analysis, set the threshold value of early warning, for example, with detect the quantity of positive sample, detect positive sample place and The setting of other relevant informations, when being more than certain threshold value, automatic early-warning;
(3) it is issued using the warning information that SMS platform, wechat platform etc. make cloud data platform, such as with short message Form is issued to inspection center responsible person, government monitoring agencies, animal doctor etc..
It is fed back by this supervision, effectively can be distributed in the inspection center of each region, detection in fact by centralized management Room is tested, and detection data is intuitive, can be traced, there is stronger practical application.

Claims (7)

1. a kind of food safety colloid gold test paper analytical equipment, which is characterized in that described device with lower unit by being constituted:
(1) data acquisition unit:
Using CCD camera, CMOS camera or the data acquisition equipment for having same function, colloidal gold strip is detected Image is acquired, and the image-region containing colloid gold test paper detection feature is transmitted to data processing and computing unit;
The data acquisition unit can be digital picture scanning means, and colloidal gold strip detection figure is acquired by way of scanning Picture or the image-region that feature is detected containing colloid gold test paper;
The image-region containing colloid gold test paper detection feature mainly includes the detection line of colloidal gold colloidal gold detection test paper strip, Quality Control The region of line;
The image-region containing colloid gold test paper detection feature can also include the spy that can identify or mark detection project Reference breath, such as the digital number information or bar code information of the stickup in colloidal gold colloidal gold detection test paper strip handheld terminal;
Accurate acquisition colloidal gold detects characteristic image region, the data acquisition unit can also include be adapted to it is various forms of The adapter of colloidal gold strip, such as the colloidal-gold detecting-card for being adapted to the colloidal gold strip of slip-type, getting stuck containing plastics Or the multi-joint strip etc. being made of several independent colloidal gold strips;
Further, in order to accurately obtain colloidal gold detection characteristic image region, the data acquisition unit can also include Fixed LED or other respective sources, for stablizing image capture environment, it is ensured that the stability of testing result;
(2) data processing and computing unit:
The data of data acquisition unit transmission are subjected to digital conversion, analyze its characteristic value, and in the way of characteristic value calculating Determine that the result of colloidal gold detection is recorded, and stores in the specific format;
In order to colloidal gold colloidal gold detection test paper strip detection project is confirmed and is calibrated in advance, the data processing and computing unit It is additionally provided with configuration data input module, wherein the data input module is the storage that can read the devices such as type-C, USB Card or ID card read equipment, two dimensional code configuration information can be read two-dimensional code scanning device, can from network obtain cloud configuration The module of information;
The configuration information of the read colloidal gold colloidal gold detection test paper strip of configuration data input module includes colloidal gold test The prefabricated detection sensitivity etc. of the prefabricated curve of detection project, testing product lot number, testing product, testing product;
The data processing and computing unit further include liquid crystal display, for showing the colloidal gold inspection of data acquisition unit input The data information of characteristic image and notebook data processing and computing unit analysis is surveyed, such as: the image containing colloidal gold detection feature Region and its characteristic value, colloidal gold testing result, sample information, operator after digital change process etc.;
Further, the data processing and computing unit further include the physical button convenient for operation, for detection process Auxiliary operation, the physical button include at least on & off switch, directionkeys, acknowledgement key;
(3) data outputting unit:
Cloud data are transmitted to using the data according to specific format storage that network handles data processing and computing unit to put down Platform, wherein the data transmitted include at least: detection device location information (IP address, GPS information etc.), contains detection time stamp There are the image-region of colloidal gold detection feature and its characteristic value after digital change process, colloidal gold testing result, sample letter Breath, operator etc.;
The data of the data outputting unit transmission further include the encrypted code containing above-mentioned detection information and its independent characteristic, with Ensure the uniqueness and unalterable feature of information, such as (IP address, GPS believe the above-mentioned detection device location information that contains of encryption Breath etc.), detection time stamp, containing colloidal gold detection feature image-region and its characteristic value after digital change process, glue Body gold testing result, sample information, operator encrypted code;
The output information of data outputting unit transmission can also pass through every food safety colloid gold test paper analytical equipment The equipment unique information such as equipment Serial Number or mac address of nic is as encryption seed;
The data processing and computing unit can also include built-in SIM card module, when Detection task is completed with short message or Person's other way notifies user, or notifies user when there is serious positive reaction result;
(4) data-printing unit:
It will test result using printer to print in the specific format, Locale Holding achieves.
2. food safety colloid gold test paper analytical equipment according to claim 1, which is characterized in that it tests and analyzes colloid The concrete operation step of the testing result of gold test paper strip includes:
Step 1: device for opening switchs, testing product project and its configuration information, example are obtained using ID card, network server end Such as enter the data processing of the present apparatus and the USB interface of computing unit using ID card insertion, data processing and computing unit can obtain automatically Take wherein configuration information;Detection project configuration information directly is obtained from network server end using data processing and computing unit Deng;
The project that selection will test from acquired project configuration information is spare;
It is slightly handled step 2: will test the colloidal gold strip finished, such as removes extra liquid using blotting paper, placed Enter in the colloidal gold strip adapter of data acquisition unit, such as is adapted to the colloidal gold strip of slip-type, contains plastic clip The colloidal-gold detecting-card of shell or the multi-joint strip etc. being made of several independent colloidal gold strips, it is ensured that colloid gold test paper The results area of item can correctly be acquired by data acquisition unit;
Step 3: data acquisition unit is by the characteristic image of automatic collection colloidal gold strip testing result detected, and with Number format is input to data processing and computing unit;Data processing and computing unit receive colloidal gold detection result image letter First pre-processed after breath, image transformation to obtain its characteristic image, and further by numerical transformation removal picture noise and Background obtains more clean image information;Continue according to preset detection project configuration information to image feature value into Row extracts, such as red (R) by obtaining picture signal, green (G), blue (B) information or tone (H), saturation degree (S), lightness (V) Information, calculates the characteristic value of colloidal gold strip detection line T line and nature controlling line C line, and further calculates its ratio;
In the presence of having standard curve, it can use standard curve and quantitative calculating carried out to unknown sample, and finally will The result output quantitatively calculated;
Step 4: the testing result that data processing and computing unit processing are completed can be exported to the display screen of this unit, It can be permanently stored while display by data outputting unit network transmission to cloud platform;The information transmitted with device from Transmitted after the sequence number or machine code encryption of body, the information transmitted include containing above-mentioned detection device location information (IP address, GPS information etc.), detection time stamp, containing colloidal gold detection feature image-region and its feature after digital change process Value, colloidal gold testing result, sample information, operator etc..
3. food safety colloid gold test paper analytical equipment according to claim 1, which is characterized in that the data processing and Computing unit (300) is calculating and the control centre of whole device, is responsible for image real time transfer analysis and other each units Control mainly includes central processing module (301), display module (302), control module (303), memory module (304), supplies Electric module (305), input module (306), wherein central processing module (301) is nucleus module, be responsible for data processing, calculating, Control program etc.;Display module (302) is the control display device being made of touch screen, for manipulating the examination of food safety colloidal gold Paper analytical equipment (100);The console that control module (303) is made of several control buttons is used for auxiliary operation food Safe colloid gold test paper analytical equipment (100);The memory that memory module (304) is made of flash memory hard disk, for storing instrument The softwares relevant informations such as device device operating system, analysis program, detection project configuration information;Power supply module (305) is by battery It with the integration module of power supply change-over device composition, is used for battery when on-site test and powers, and connected when battery supply feed with power supply line Common power power supply;Input module (306) is to read the project configuration in USB flash memory by the incoming device with input interface Information, such as bulleted list, item argument, product batches information are transported for food safety colloid gold test paper analytical equipment (100) Row.
4. food safety colloid gold test paper analytical equipment according to claim 1, which is characterized in that colloid in described device Gold test paper strip characteristic image extracts and analysis process is as follows:
(1) characteristic area is searched: the colloidal gold strip testing result characteristic image acquired using data acquisition unit (200), It is handled through data processing and computing unit (300), such as through binary transform, searches boundary etc., determine colloidal gold strip The characteristic area of testing result;
(2) it cuts: continuing to carry out the characteristic area of lookup fixed point and cut, it is ensured that the integrality of detection information and interference information It minimizes;
(3) data analysis and transformation: acquisition image pixel data matrix is progressively scanned or scanned by column using image pixel, to it In characteristic extract, such as by analysis by ready image data matrix, extract its R, G, certain in channel B One channel data perhaps the average value in three channels or extracts the wherein data of its H, S, V data, utilizes its drafting The one kind of pixel change curve as output information;
(4) calculate characteristic value: utilize filter process pixel data, reduce noise, improve signal-to-noise ratio, by the data handled well into The analysis of one step calculates colloidal gold colloidal gold detection test paper strip T line (including single T line to more T lines), (including single C line is to more C for C line Line) characteristic parameter;
The characteristic value includes the peak-peak of T line, average peak, peak area, the peak-peak of C line, peak area, peak area Deng;
(5) testing result is calculated:
The various characteristic parameters calculated in (4) are utilized, compare the characteristic parameter of T line Yu C line, such as calculate its character pair parameter Ratio marks as a result, compares with configuration information, determines testing result;
When, there are when standard curve information, can further utilizing cubic spline curve, 4 parameter logistic curves, 5 in configuration information Parameter logistic curve, even numbers curve etc. carry out data fitting, calculate quantitative detection result.
5. food safety colloid gold test paper analytical equipment according to claim 4, which is characterized in that colloid in described device Gold test paper strip testing result characteristic value data acquisition range is the area of vertical width 5% to 95% in colloidal gold strip result images The range of domain range, the C line or T line acquisition value is the regional scope of its vertical width 5% to 95%.
6. food safety colloid gold test paper analytical equipment according to claim 5, which is characterized in that colloid in described device The calculation of gold test paper strip testing result characteristic value is as follows:
(1) according to selected colloidal gold strip result images, longitudinal pixel average is calculated, draws average value along image transverse direction The curve of distribution, since C line region and T line region are since pixel color is compared with remaining region of test strips depth, obtained curve be should be Curve with several troughs, and trough quantity is identical as the sum of T line number amount and C line number amount;
(2) position of trough, quantity on the curve drawn are determined, determines the position range of C line, the position range and quantity of T line, And the image feature value in 5% ~ 95% region within the scope of C, T line is calculated, the characteristic value includes C, T line in the flat of the wave trough position Mean value, extreme value, trough area;
(3) calculate T line and C line individual features value ratio, as toaverage ratio, the ratio of extreme value, area ratio;
(4) positive and negative of final detection result are determined with preset threshold value comparison according to the ratio of calculating.
7. food safety colloid gold test paper analytical equipment according to claim 6, which is characterized in that tried using colloidal gold The standard solution (S1, S2, S3, S4, S5, S6) for the gradient concentration that paper slip detection is prepared in advance obtains testing result characteristic value note For T1, T2, T3, T4, T5, T6, its concentration, the corresponding relationship curve matching of characteristic value are further utilized, it is quantitative to unknown sample Detection.
CN201811257592.XA 2018-10-26 2018-10-26 A kind of food safety colloid gold test paper analytical equipment and its application Pending CN109146882A (en)

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Application publication date: 20190104