CN102879568A - Poison four-in-one joint detection test card - Google Patents
Poison four-in-one joint detection test card Download PDFInfo
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- CN102879568A CN102879568A CN2011102003937A CN201110200393A CN102879568A CN 102879568 A CN102879568 A CN 102879568A CN 2011102003937 A CN2011102003937 A CN 2011102003937A CN 201110200393 A CN201110200393 A CN 201110200393A CN 102879568 A CN102879568 A CN 102879568A
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Abstract
The invention belongs to the technical field of biology and new medicine and pharmacology. The invention adopts an integrated technical conception that a colloidal gold detection test card serves as a detection method which is simple, quick, low in cost and easy to operate; the color of colloidal gold can be identified by naked eyes; a result can be directly observed without secondary amplification; and antigens and antibodies are high in specificity. High-specificity antibody-antigen reaction is combined with an immune colloidal gold chromatographic technology, a joint detection test card of four poisons is prepared, and the defects of complexity in operation, high cost and the like of a single test card are overcome. The joint test card has the characteristics of quickness, sensitivity and low cost, is easy to operate, and can be used for detection without professionals.
Description
Technical field
The invention belongs to pharmaceutical sanitary field, can be used for simultaneously public security system, army's conscription, narcotic house, labour education and rehabilitation school, family's monitoring, community's drug rehabilitation, etc. administrative department.
Technical background
Global overflow of drugs has become the harm people ' s health and has affected one of key factor of social stability.All seeking a kind of simple and easy, method of inspection fast and accurately, so that generally investigate (such as conscription, public place of entertainment etc.) in the specific occasion, reach the purpose that prevents that drugs from further spreading both at home and abroad.At present illicit drugs inspection mainly is to carry out urine detection as main take gold mark method, and principal feature is fast, make things convenient for, be easy to carry, the accuracy rate high.The method that the mensuration of single drugs more generally adopts in the urine has: monoclonal antibody monolithic qualitative method, thin-layer chromatography chromatography, vapor-phase chromatography, gas-matter coupling method.All there are the weak points such as the many and pretreatment process of cross reaction, disturbing factor is complicated in these methods in varying degrees.The immuno-gold labeling technology be with collaurum as tracer label thing or developer, be applied to a kind of Novel immune labelling technique of antigen-antibody reaction.Because there are not the problems such as endogenous enzymes interference and radioisotope pollution in it, and utilizes the collaurum of variable grain size can also make dual even multiple labelling, makes the location more accurate.Monoclonal antibody has high specificity, the advantages such as sensitivity height.
Summary of the invention
The object of the present invention is to provide and a kind ofly can detect simultaneously whether contain morphine, methamphetamine, head-shaking pill, these four kinds of compositions of KET in the urine sample, and can accurate, quick, simple, portative product.
Overall technology of the present invention design is: collaurum inspection test card is a kind of detection method of easy, quick, cheap easy operating, and the color of collaurum can with the naked eye distinguish, need not that secondary amplifies can direct observations, and antigen-antibody has high specific.The antibody antigen reaction of high degree of specificity is combined with the immune colloidal gold chromatography technology, and the joint-detection examination card of four kinds of drugs of preparation has remedied the shortcomings such as single test card complex operation, somewhat expensive.This unites, and the examination jig has fast, sensitive, easy and simple to handle, cost is low, need not the characteristics that the professional detects.
Drugs four unification joint-detection examination card embodiments:
(1) with the antigen immune BALB/C mouse of four kinds of drugs such as morphine, prepare monoclonal antibody, and antibody purification.
(2) preparation gold grain, colloidal gold labeled monoclonal antibody, envelope antigen, antibody, the examination card is united in preparation.
Collaurum examination blocking Preparation Method is: get gold chloride and make sizeable gold grain, and four kinds of antigens such as morphine are carried out mark with gold grain respectively; Antigen coated: as the mark colloidal gold antigen to be coated on the glass fibre element film vacuum drying; Antibody sandwich: will resist McAb and the sheep anti-mouse igg antibody of four kinds in morphine etc. to be coated on the cellulose acetate membrane vacuum drying with automatic Membrane jetter; Two kinds of films are affixed on the magnetic blank with a definite sequence.
The examination card comprises plastic plate and illicit drugs inspection test paper; Plastic plate comprises plate body and Ban Gai, and plate body inside is provided with 4 grooves corresponding with the illicit drugs inspection test paper, and plate body front has the result to differentiate the explanation of explanation, detected object and display field as a result, and it is inner that 4 on illicit drugs inspection test paper is fixed in the plate body.Test strips inclusion test sample areas, testing result viewing area and suction zone.This examination card can detect the composition that whether contains morphine, methamphetamine, head-shaking pill, KET in the urine sample simultaneously, and accuracy and reliability are high, and testing process is simple, and speed is fast, is convenient for carrying.
A kind of drugs four unification joint-detection plates is characterized in that comprising plastic plate, illicit drugs inspection test paper; Plastic plate comprises plate body and plate lid two parts, and plate body and plate lid connected mode are for pegging graft; Plate body inside is provided with the groove corresponding with the illicit drugs inspection test paper, and plate body front has the result to differentiate the explanation of explanation, detected object and display field as a result; It is inner that the illicit drugs inspection test paper is fixed in the plate body, formed by base plate, water sucting plate, nitrocellulose filter, monoclonal antibody gold mark pad, sample liquid-adsorption layer, MAX line, the base plate middle part is nitrocellulose filter, base plate one end termination is water sucting plate, other end termination is the sample liquid-adsorption layer, being connected with monoclonal antibody mark mutual overlapping connection of pad with water sucting plate respectively in the nitrocellulose filter two ends, is pressed with the sample liquid-adsorption layer at monoclonal antibody gold mark pad.
Claims (2)
1. drugs four unification joint-detection are tried the preparation method of card, it is characterized in that it comprises following processing step:
(1) with the diazotising method haptens morphine, methamphetamine, head-shaking pill, KET are coupled to respectively on the carrier protein, form coupled antigen.
(2) with the coupled antigen of the four kinds of drugs such as morphine in (1) immunity BALB/C mouse, measure serum antibody titer with the ELISA method, tiring namely can be used for Fusion of Cells after reaching requirement.
(3) preparation hybridoma.
(4) preparation, the purifying of four kinds of drugs monoclonal antibodies such as morphine.
(5) preparation of the drugs such as morphine four unification joint-detection examination cards: get gold chloride and make sizeable gold grain, and four kinds of antigens such as morphine that will (1) middle coupling carry out mark with gold grain respectively; Antigen coated colloidal gold antigen with mark is coated on the NC film, vacuum drying; With automatic Membrane jetter McAb and the sheep anti-mouse igg antibody of four kinds in the anti-morphine in (4) etc. is coated on the glass fibre element film vacuum drying; Two kinds of films are affixed on the magnetic blank with a definite sequence.
2. the preparation method of drugs four unification joint-detection examination cards according to claim 1 is characterized in that four kinds of haptenic couplings of drugs such as (1) morphine; (4) preparation, the purifying of four kinds of drugs monoclonal antibodies such as morphine; (5) preparations of four kinds of drugs such as morphine, four unification joint-detection examination cards.The examination card comprises plastic plate and illicit drugs inspection test paper; Plastic plate comprises plate body and Ban Gai, and plate body inside is provided with 4 grooves corresponding with the illicit drugs inspection test paper, and plate body front has the result to differentiate the explanation of explanation, detected object and display field as a result, and it is inner that 4 on illicit drugs inspection test paper is fixed in the plate body.Test strips inclusion test sample areas, testing result viewing area and suction zone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102003937A CN102879568A (en) | 2011-07-12 | 2011-07-12 | Poison four-in-one joint detection test card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102003937A CN102879568A (en) | 2011-07-12 | 2011-07-12 | Poison four-in-one joint detection test card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102879568A true CN102879568A (en) | 2013-01-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011102003937A Pending CN102879568A (en) | 2011-07-12 | 2011-07-12 | Poison four-in-one joint detection test card |
Country Status (1)
| Country | Link |
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| CN (1) | CN102879568A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104101710A (en) * | 2014-04-02 | 2014-10-15 | 浙江省海洋开发研究院 | Detection method and detection kit for seawater fish endogenous morphine |
| CN104404001A (en) * | 2014-11-13 | 2015-03-11 | 中国科学院生物物理研究所 | Anti 2-bromopropiophenone monoclonal antibody and application thereof |
| CN105527422A (en) * | 2016-01-18 | 2016-04-27 | 北京亦庄国际诊断试剂技术有限公司 | Test paper for three drugs in saliva and preparation method thereof |
| TWI801979B (en) * | 2021-08-26 | 2023-05-11 | 國立臺灣大學 | Detection kit and method for detecting abused drugs |
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| US20010026944A1 (en) * | 1999-04-21 | 2001-10-04 | Roy Chung | Immunoassay system |
| CN1632583A (en) * | 2004-12-30 | 2005-06-29 | 上海美康生物工程有限公司 | Multi-in-one combined detection kit for drug and preparation process and enclosed reagent thereof |
| CN2715155Y (en) * | 2004-05-21 | 2005-08-03 | 王继华 | Reagent kit for multi-index joint inspection |
| CN200968955Y (en) * | 2006-07-26 | 2007-10-31 | 艾博生物医药(杭州)有限公司 | Biological liquid sample analytical equipment |
| CN101241136A (en) * | 2007-11-30 | 2008-08-13 | 广州万孚生物技术有限公司 | Multi-linked immunity chromatography test paper for detecting saliva abusing drug, system and its preparation method |
| CN201402263Y (en) * | 2009-03-31 | 2010-02-10 | 北京库尔科技有限公司 | Six-in-one detection reagent card for drug abuse |
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2011
- 2011-07-12 CN CN2011102003937A patent/CN102879568A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20010026944A1 (en) * | 1999-04-21 | 2001-10-04 | Roy Chung | Immunoassay system |
| CN2715155Y (en) * | 2004-05-21 | 2005-08-03 | 王继华 | Reagent kit for multi-index joint inspection |
| CN1632583A (en) * | 2004-12-30 | 2005-06-29 | 上海美康生物工程有限公司 | Multi-in-one combined detection kit for drug and preparation process and enclosed reagent thereof |
| CN200968955Y (en) * | 2006-07-26 | 2007-10-31 | 艾博生物医药(杭州)有限公司 | Biological liquid sample analytical equipment |
| CN101241136A (en) * | 2007-11-30 | 2008-08-13 | 广州万孚生物技术有限公司 | Multi-linked immunity chromatography test paper for detecting saliva abusing drug, system and its preparation method |
| CN201402263Y (en) * | 2009-03-31 | 2010-02-10 | 北京库尔科技有限公司 | Six-in-one detection reagent card for drug abuse |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104101710A (en) * | 2014-04-02 | 2014-10-15 | 浙江省海洋开发研究院 | Detection method and detection kit for seawater fish endogenous morphine |
| CN104101710B (en) * | 2014-04-02 | 2016-02-03 | 浙江省海洋开发研究院 | The detection method of raw morphine and detection kit thereof in a kind of fish |
| CN104404001A (en) * | 2014-11-13 | 2015-03-11 | 中国科学院生物物理研究所 | Anti 2-bromopropiophenone monoclonal antibody and application thereof |
| CN104404001B (en) * | 2014-11-13 | 2017-03-15 | 中国科学院生物物理研究所 | A kind of monoclonal antibody of anti-2 brom-acetophenones and its application |
| CN105527422A (en) * | 2016-01-18 | 2016-04-27 | 北京亦庄国际诊断试剂技术有限公司 | Test paper for three drugs in saliva and preparation method thereof |
| TWI801979B (en) * | 2021-08-26 | 2023-05-11 | 國立臺灣大學 | Detection kit and method for detecting abused drugs |
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Application publication date: 20130116 |