CN203083999U - Gold immnnochromatography sperm density detection kit - Google Patents
Gold immnnochromatography sperm density detection kit Download PDFInfo
- Publication number
- CN203083999U CN203083999U CN 201320043407 CN201320043407U CN203083999U CN 203083999 U CN203083999 U CN 203083999U CN 201320043407 CN201320043407 CN 201320043407 CN 201320043407 U CN201320043407 U CN 201320043407U CN 203083999 U CN203083999 U CN 203083999U
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- CN
- China
- Prior art keywords
- monoclonal antibody
- plastic gasket
- sample pad
- box body
- nitrocellulose membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Abstract
The utility model discloses a gold immnnochromatography sperm density detection kit which comprises a clamp box formed by matching a box cover and a box body and a reagent strip clamped in the box body through a fixing protruding member, wherein the bottom layer of the reagent strip is a plastic gasket; a section of sample pad is attached to one end of the plastic gasket; a gold immnnochromatography pad coated with a mouse anti-human SP10 monoclonal antibody is attached to the sample pad; a section of nitrocellulose membrane partially embedded into the sample pad and attached to the plastic gasket is adjacent to the sample pad; the nitrocellulose membrane is coated with a detection T line containing the SP10 monoclonal antibody and a quality control C line containing the IgG monoclonal antibody; the nitrocellulose membrane is also partially embedded into an adjacent water absorption pad attached to the other end of the plastic gasket; and a sampling hole and a display window for displaying the test result are formed in the box cover. The kit disclosed by the utility model is sensitive and specific and convenient and quick, and has high accuracy; and the detection result can be accurately obtained in a short time.
Description
Technical field
The utility model relates to a kind of in-vitro diagnosis class medicine equipment, particularly relates to a kind of kit that sperm concentration detects that is used for that adopts golden mark method.
Background technology
About 15% Mr. and Mrs can not be conceived in 1 year in the world today, and 40% in these cases are because male factor causes.Also have the Andriatrics Dept. expert to report for work, the incidence of disease of male sterility is approximately 6-12%.The above-mentioned crowd who reports for work the explanation male sterility is a sizable crowd.The main method of current diagnosis male sterility is the microscopically diagnosis, be that sperm morphological detects, this also is the standard of male sterility diagnosis, but there are many shortcomings in this method, for example need to go diagnosis and treatment to hospital, even gone to hospital, also, be subjected to the restriction of environment, method, time because the condition of sampling is limited, be difficult for obtaining sample, therefore, receive the diagnosis and treatment of male sterility owing to be subjected to subjective and objectively restriction, thereby delayed treatment.
Some sperm concentration self check instruments have been developed at present, as adopting the kit that sperm concentration detects that is used for of colourimetry, this product can use at home, has characteristics such as quick, easy to use, but when detecting sperm concentration owing to the employing colourimetry, can not distinguish non-spermatoblast composition, specificity is not strong, and the operation steps of the method is many, length consuming time, easily make mistakes, error in judgement is interval big and can not be quantitative as a result, so it is restricted when reality is used.
The utility model content
The purpose of this utility model is in order to overcome the shortcoming of prior art, to provide a kind of and adopt golden mark method to detect the kit of sperm concentration, this kit has simply, flexibly, fast, convenient, advantage accurately.
The purpose of this utility model can be achieved through the following technical solutions:
A kind of golden mark method sperm concentration detection kit is characterized in that, comprises by lid and box body cooperating the cartridge that forms, and is installed on reagent strip in the box body by fixing with protrusion; The bottom of described reagent strip is a plastic gasket, and an end of described plastic gasket is pasted with one section sample pad, is pasted with the gold mark pad that is coated with mouse-anti people SP10 monoclonal antibody on sample pad; Next-door neighbour's sample is lined with one section and is partially submerged into sample pad and is attached to nitrocellulose membrane on the plastic gasket; Be coated with detection T line that contains rabbit anti-people SP10 polyclonal antibody or mouse-anti people SP10 monoclonal antibody and the Quality Control C line that contains goat anti-rabbit igg monoclonal antibody or sheep anti-mouse igg monoclonal antibody on the described nitrocellulose membrane; Described nitrocellulose membrane also be partially submerged into the next-door neighbour it and be attached to adsorptive pads on the plastic gasket other end; The position of the interior reagent strip sample pad of corresponding box body has a well on the described lid; The detection T line and the Quality Control C line position of the interior reagent strip of corresponding box body have a display window that is used for showing test results on the described lid.
Kit of the present utility model adopts a pair of monoclonal antibody composition double antibody or adopts resist with monoclonal antibody composition double antibody sandwich method to detect sperm concentration more, and is not only sensitive, special, and fast, the accuracy rate height, measurement result can accurately obtain at short notice.In addition, kit of the present utility model only needs to drip sample liquid in use in well and gets final product, need not as the available reagent bar, the whole piece reagent strip be put into the test tube that fills sample liquid and be detected, make the utility model simple more, convenient from use-pattern like this.
Description of drawings
Fig. 1 is the vertical view of the utility model kit.
Fig. 2 is the lid structural representation of the utility model kit.
Fig. 3 is the box body inner structure synoptic diagram of the utility model kit.
Fig. 4 is the cross-sectional view of the utility model kit.
Be labeled as among the figure: the 1-cartridge, the 11-lid, the 12-box body, the 2-reagent strip, the 21-nitrocellulose membrane, the 22-sample pad, the 23-adsorptive pads, 24-detects the T line, 25-Quality Control C line, 26-gold mark pad, the 27-plastic gasket, 3-is fixing to use projection piece, 4-well, 5-display window.
Embodiment
Below in conjunction with drawings and Examples the utility model is done and to be described in further detail.
Shown in Fig. 1-4, a kind of golden mark method sperm concentration detection kit comprises by lid 11 and box body 12 cooperating the cartridge 1 that forms, and is installed on the interior reagent strip 2 of box body 12 by fixing with protrusion 3; The bottom of described reagent strip 2 is a plastic gasket 27, and an end of described plastic gasket 27 is pasted with one section sample pad 22, is pasted with the gold mark pad 26 that is coated with mouse-anti people SP10 monoclonal antibody on sample pad 22; Next-door neighbour's sample pad 22 has one section to be partially submerged into sample pad 22 and to be attached to nitrocellulose membrane 21 on the plastic gasket 27; Be coated with detection T line 24 that contains rabbit anti-people SP10 polyclonal antibody or mouse-anti people SP10 monoclonal antibody and the Quality Control C line 25 that contains goat anti-rabbit igg monoclonal antibody or sheep anti-mouse igg monoclonal antibody on the described nitrocellulose membrane 21; Described nitrocellulose membrane 21 also be partially submerged into the next-door neighbour it and be attached to adsorptive pads 23 on plastic gasket 27 other ends; The position of corresponding box body 12 interior reagent strip sample pad 22 has a well 4 on the described lid 11; The detection T line 24 and Quality Control C line 25 positions of corresponding box body 12 interior reagent strips 2 have a display window that is used for showing test results 5 on the described lid 11.
When stating kit in the use, as long as will be in advance in the spermatoblast lysis buffer sample of cracking splash in the well 4 of kit, be dissolved in after SP10 in the sample dissociation damping fluid enters the sample application zone of reagent strip, gold mark pad 26(colloid gold label) mouse-anti people SP10 monoclonal antibody combines with the SP10 antigen in the sample, formed Ag-Ab colloidal gold composite moves at capillary effect lower edge nitrocellulose membrane 21, combines with immobilization rabbit anti-people SP10 polyclonal antibody or mouse-anti people SP10 monoclonal antibody at detection T line 24 places and forms a red detection line; The polyclonal antibody of unnecessary colloid gold label or monoclonal antibody then continue to move to Quality Control C line 25 places, form another red nature controlling line with goat-anti rabbit or sheep anti-mouse igg.As no SP10 antigen in the sample, then can not form red detection line, only can be observed a red nature controlling line.
The above; it only is the preferable embodiment of the utility model; but protection domain of the present utility model is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the utility model discloses; the variation that can expect easily or replacement all should be encompassed within the protection domain of the present utility model.
Claims (1)
1. a golden mark method sperm concentration detection kit is characterized in that, comprises by lid and box body cooperating the cartridge that forms, and is installed on reagent strip in the box body by fixing with protrusion; The bottom of described reagent strip is a plastic gasket, and an end of described plastic gasket is pasted with one section sample pad, is pasted with the gold mark pad that is coated with mouse-anti people SP10 monoclonal antibody on sample pad; Next-door neighbour's sample is lined with one section and is partially submerged into sample pad and is attached to nitrocellulose membrane on the plastic gasket; Be coated with detection T line that contains rabbit anti-people SP10 polyclonal antibody or mouse-anti people SP10 monoclonal antibody and the Quality Control C line that contains goat anti-rabbit igg monoclonal antibody or sheep anti-mouse igg monoclonal antibody on the described nitrocellulose membrane; Described nitrocellulose membrane also be partially submerged into the next-door neighbour it and be attached to adsorptive pads on the plastic gasket other end; The position of the interior reagent strip sample pad of corresponding box body has a well on the described lid; The detection T line and the Quality Control C line position of the interior reagent strip of corresponding box body have a display window that is used for showing test results on the described lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320043407 CN203083999U (en) | 2013-01-25 | 2013-01-25 | Gold immnnochromatography sperm density detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320043407 CN203083999U (en) | 2013-01-25 | 2013-01-25 | Gold immnnochromatography sperm density detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203083999U true CN203083999U (en) | 2013-07-24 |
Family
ID=48829824
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320043407 Expired - Lifetime CN203083999U (en) | 2013-01-25 | 2013-01-25 | Gold immnnochromatography sperm density detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203083999U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104459152A (en) * | 2014-12-03 | 2015-03-25 | 青岛拓新生物科技有限公司 | Rapid sperm concentration detection method based on colloidal gold immunochromatograohic assay technology |
| CN105675890A (en) * | 2015-12-31 | 2016-06-15 | 湖南农业大学 | Apparatus for detecting pregnancy of laboratory mice, and method thereof |
| CN108548928A (en) * | 2018-04-09 | 2018-09-18 | 无锡全策生物科技有限公司 | A kind of kit and detection method of quick detection spermatozoon activity |
-
2013
- 2013-01-25 CN CN 201320043407 patent/CN203083999U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104459152A (en) * | 2014-12-03 | 2015-03-25 | 青岛拓新生物科技有限公司 | Rapid sperm concentration detection method based on colloidal gold immunochromatograohic assay technology |
| CN105675890A (en) * | 2015-12-31 | 2016-06-15 | 湖南农业大学 | Apparatus for detecting pregnancy of laboratory mice, and method thereof |
| CN108548928A (en) * | 2018-04-09 | 2018-09-18 | 无锡全策生物科技有限公司 | A kind of kit and detection method of quick detection spermatozoon activity |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20161014 Address after: 200032 Shanghai Xuhui District Xietu Road No. 2140 Patentee after: SHANGHAI INSTITUTE OF PLANNED PARENTHOOD RESEARCH Address before: 200032 Shanghai Xuhui District Xietu Road No. 2140 Patentee before: SHANGHAI HUIBAO BIO-TECHNOLOGY Co.,Ltd. |
|
| CP03 | Change of name, title or address |
Address after: 200032 Shanghai Xuhui District Xietu Road No. 2140 Patentee after: Shanghai Institute of biomedical technology Address before: 200032 Shanghai Xietu Road No. 2140 Patentee before: SHANGHAI INSTITUTE OF PLANNED PARENTHOOD RESEARCH |
|
| CP03 | Change of name, title or address | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130724 |
|
| CX01 | Expiry of patent term |