CN211263495U - Multi-directional combined detection test strip - Google Patents
Multi-directional combined detection test strip Download PDFInfo
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- CN211263495U CN211263495U CN201922093794.1U CN201922093794U CN211263495U CN 211263495 U CN211263495 U CN 211263495U CN 201922093794 U CN201922093794 U CN 201922093794U CN 211263495 U CN211263495 U CN 211263495U
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Abstract
The utility model relates to an immunochromatography short-term test field, concretely relates to multidirectional joint test paper strip to the detailed two-way joint test paper strip that discloses, the test paper strip by PVC bottom plate, sample pad, combination pad, reaction pad and the pad that absorbs water constitute, sample pad, combination pad, reaction pad and the pad that absorbs water overlap joint on the bottom plate in proper order, the test paper strip have a plurality of different flow directions, can realize jointly detecting the different indexes of a same sample, also avoided the mutual interference between the different indexes simultaneously, also can realize the repeated detection to a same sample to can increase the accuracy of testing result. Especially for samples which are not easy to collect and groups which are not easy to collect samples, such as blood samples of infants and young children. Compare in the two index test paper strips of linear type, the utility model discloses a test paper strip can avoid the error that causes because of the different or different of application of sample time of the volume of adding sample, also can avoid the uneven condition of sample, has increased the accuracy of testing result.
Description
Technical Field
The utility model belongs to immunochromatography short-term test field, concretely relates to multidirectional joint detection test strip.
Background
Nowadays, the immunochromatography technology is a rapid detection technology widely used at home and abroad, is widely applied to various detection fields such as medicine, food, agriculture, animal husbandry and the like, and has a wide development prospect. On the immunochromatographic strip, an antibody (or antigen) that reacts with an antigen (or antibody) in an analyte to be detected to produce an antigen-antibody reaction is coated in advance on a specific position of the immunochromatographic strip. After the analyte is added to the immunochromatographic test strip, the antigen (or antibody) in the test body is eluted and impregnated on the test strip by the developing solution, and the antigen (or antibody) is captured and developed at the antibody (or antigen) coated on the immunochromatographic test strip by the antigen-antibody reaction. Since the amount of the captured antigen (or antibody) is the total amount of the antigen (or antibody) in the specimen, the immunochromatographic assay is a method capable of quantifying an extremely small amount as compared with a usual chromogenic assay. With the development of the technology, the requirements of people on the technology are higher and higher, the single detection of partial molecules cannot meet the requirements, and the emergence of the combined detection test strip promotes the further wide application of the immunochromatography technology.
The invention discloses a time-resolved fluorescence immunochromatographic test strip and a kit for combined detection of CA19-9 and CEA (CN201810135356.4), the test strip comprises a bottom plate, a nitrocellulose membrane, glass fiber cotton and absorbent paper, the nitrocellulose membrane is coated with a CA19-9 monoclonal antibody as a detection 1 line (T1 line), a CEA monoclonal antibody as a detection 2 line (T2 line) and an anti-mouse polyclonal antibody as a quality control line (C line), the glass fiber cotton is divided into a sampling region and a binding region, and the binding region is sprayed with a CA19-9 monoclonal antibody compound and a CEA monoclonal antibody compound marked by time-resolved fluorescence microspheres. The time-resolved fluorescence immunochromatographic test strip and the kit for the combined detection of CA19-9 and CEA can complete the concentration detection of CA19-9 and CEA in a short time, have high accuracy and good sensitivity of detection results, and can meet the requirements of clinical bedside rapid detection and basic community hospitals. However, in the technical scheme, different samples are detected on the same test strip, the same control line and the two detection lines are arranged, the two detection lines detect different markers respectively, and the detection effect of the second detection line is easily influenced after the first detection line reacts because the markers are mixed together. Aiming at the technical problem, the technical scheme is provided with the multidirectional joint detection test strip.
SUMMERY OF THE UTILITY MODEL
Multidirectional a two multidirectional joint detection test paper strips, the test paper strip by PVC bottom plate 1, sample pad 2, combination pad 3, reaction pad 4 and absorb water and fill up 5 and constitute, sample pad 2, combination pad 3, reaction pad 4 and absorb water and fill up 5 and arrange in proper order on PVC bottom plate 1, PVC bottom plate 1 use the position at sample pad 2 place to be equipped with the branch bottom plate as the center, the branch bottom plate to be radial extension all around, the quantity of branch bottom plate be 2-10.
Preferably, the test strip includes 2 branch bottom plates, which are a first branch bottom plate 101 and a second branch bottom plate 102; the bonding pad 3 comprises a first bonding pad 301 and a second bonding pad 302; the reaction pad 4 comprises a first reaction pad 401 and a second reaction pad 402; the absorbent pad 5 comprises a first absorbent pad 501 and a second absorbent pad 502; the sample pad 2, the first combination pad 301, the first reaction pad 401 and the first water absorption pad 501 are sequentially overlapped on the first branch bottom plate 101, have the same width as the first branch bottom plate 101, and cover the whole first branch bottom plate 101 after being arranged; the sample pad 2, the second combination pad 302, the second reaction pad 402 and the second absorbent pad 502 are sequentially lapped on the second branch bottom plate 102, have the same width as the second branch bottom plate 102, and cover the whole second branch bottom plate 102 after being arranged; the first branch bottom plate 101 and the second branch bottom plate 102 are connected at the position of the sample pad 2, and the connection position has an included angle, and the degree of the included angle is 10-90 degrees; the first reaction pad 401 is provided with a first detection belt 7 and a first control belt 8, the first detection belt 7 is arranged at one end of the first reaction pad 401 close to the first combination pad 301, and the first control belt 8 is arranged at one end of the first reaction pad 401 close to the first water absorption pad 501; the second reaction pad 402 is provided with a second detection zone 9 and a second control zone 10, the second detection zone 9 is disposed at one end of the second reaction pad 402 close to the second bonding pad 302, and the second control zone 10 is disposed at one end of the second reaction pad 402 close to the second absorbent pad 502.
Preferably, the first detection zone 7 is coated with an anti-a antibody; the second detection zone 9 is coated with an anti-B antibody; the first control band 8 and the second control band 10 are coated with immunoglobulin.
Preferably, the first bonding pad 301 has an anti-a antibody complex immobilized thereon, and the second bonding pad 302 has an anti-B antibody complex immobilized thereon.
Preferably, the nanoprobes used for the anti-a antibody complex and the anti-B antibody complex are the same nanoprobe or different nanoprobes.
Preferably, the anti-a antibody coated on the first detection zone 7 is different from the anti-a antibody immobilized on the first conjugate pad 301; the second detection zone 9 is coated with an anti-B antibody that is different from the anti-B antibody immobilized on the second conjugate pad 302.
Preferably, the spacing between the first detection zone 7 and the first control zone 8 is equal to the spacing between the second detection zone 9 and the second control zone 10, and the spacing is 2-4 mm.
Preferably, the first reaction pad 401 and the second reaction pad 402 are respectively provided with a nitrocellulose membrane with the same size and specification, and the detection tape and the control tape are coated on the nitrocellulose membrane.
Preferably, the joining length of the sample pad 2 and the combination pad 3 is 1-2mm, the joining length of the combination pad 3 and the reaction pad 4 is 1-2mm, and the joining length of the reaction pad 4 and the absorbent pad 5 is 1-2 mm.
The utility model has the advantages that: the utility model provides a multidirectional joint detection test paper strip specifically provides a multidirectional joint detection test paper strip, multidirectional test paper strip has two different flow directions, and two sample pads that flow to share can realize jointly detecting the different indexes of a same sample, have also avoided the interference of each other between the different indexes simultaneously, also can realize the repeated detection to a same sample to can increase the accuracy of testing result. Especially for samples which are not easy to collect and groups which are not easy to collect samples, such as blood samples of infants and young children. Compare in the two index test paper strips of linear type, the utility model discloses a test paper strip can avoid the error that causes because of the different or different of application of sample time of the volume of adding sample, also can avoid the uneven condition of sample, has increased the accuracy of testing result.
Drawings
FIG. 1 is a front view of a multi-directional joint test strip
1. A base plate; 101. a first base plate; 102. a second base plate; 2. a sample pad; 3. a bonding pad; 301. a first bond pad; 302. a second bonding pad; 4. a reaction pad; 401. a first reaction pad; 402. a second reaction pad; 5. a water absorbent pad; 501. a first water absorption pad; 502. a second absorbent pad; 7. a first detection zone; 8. a first control band; 9. a second detection zone; 10. second control band
FIG. 2 is a top view of a multi-directional joint test strip
Detailed Description
The present invention will be further described with reference to the accompanying drawings and examples, but the description is only for explaining the technical solution of the present invention and does not limit the protection scope of the present invention.
Embodiment I, a multidirectional joint detection test strip
A multi-directional combined detection test strip is composed of a PVC base plate 1, a sample pad 2, a combination pad 3, a reaction pad 4 and a water absorption pad 5, wherein the PVC base plate 1 comprises a first branch base plate 101 and a second branch base plate 102; the bonding pad 3 comprises a first bonding pad 301 and a second bonding pad 302; the reaction pad 4 comprises a first reaction pad 401 and a second reaction pad 402; the absorbent pad 5 comprises a first absorbent pad 501 and a second absorbent pad 502; the sample pad 2, the first combination pad 301, the first reaction pad 401 and the first water absorption pad 501 are sequentially overlapped on the first branch bottom plate 101, have the same width as the first branch bottom plate 101, and cover the whole bottom plate 101 after being arranged; the sample pad 2, the second combination pad 302, the second reaction pad 402 and the second absorbent pad 502 are sequentially lapped on the second branch bottom plate 102, have the same width as the second branch bottom plate 102, and cover the whole second branch bottom plate 102 after being arranged; the first branch bottom plate 101 and the second branch bottom plate 102 are connected at the position of the sample pad 2, and the joint has an included angle with the degree of 90 degrees; the first reaction pad 401 is provided with a first detection belt 7 and a first control belt 8, the first detection belt 7 is arranged at one end of the first reaction pad 401 close to the first combination pad 301, and the first control belt 8 is arranged at one end of the first reaction pad 401 close to the first water absorption pad 501; the second reaction pad 402 is provided with a second detection zone 9 and a second control zone 10, the second detection zone 9 is disposed at one end of the second reaction pad 402 close to the second bonding pad 302, and the second control zone 10 is disposed at one end of the second reaction pad 402 close to the absorbent pad 502. The first detection zone 7 is coated with an anti-A antibody; the second detection zone 9 is coated with an anti-B antibody; the first control band 8 and the second control band 10 are coated with goat anti-mouse IgG. The first bonding pad 301 has an anti-a antibody complex immobilized thereon, and the second bonding pad 302 has an anti-B antibody complex immobilized thereon. The nanoprobes used by the anti-A antibody compound and the anti-B antibody compound are the same nanoprobe. The first detection zone 7 coated with the anti-A antibody is different from the anti-A antibody immobilized on the first conjugate pad 301; the second detection zone 9 is coated with an anti-B antibody that is different from the anti-B antibody immobilized on the second conjugate pad 302. The spacing between the first detection zone 7 and the first control zone 8 is equal to the spacing between the second detection zone 9 and the second control zone 10, and the spacing is 2 mm. The first reaction pad 401 and the second reaction pad 402 are respectively provided with a nitrocellulose membrane with the same specification and size, and the detection tape and the control tape are coated on the nitrocellulose membrane. Sample pad 2 and combination pad 3 link up length and be 2mm, combination pad 3 and reaction pad 4 link up length and be 2mm, reaction pad 4 and 5 link up length of absorbing water are 2 mm.
Second embodiment, a method for using a multi-directional joint detection test strip
(1) The first binding pad 301 is added with the anti-A antibody complex, and the second binding pad 302 is added with the anti-B antibody complex;
(2) the first detection zone 7 is coated with an anti-a antibody different from the anti-a antibody complex immobilized on the first conjugate pad 301, the second detection zone 9 is coated with an anti-B antibody different from the anti-B antibody complex immobilized on the second conjugate pad 302, and the first control zone 8 and the second control zone 10 are coated with goat anti-mouse IgG.
(3) After the test strip is prepared, a sample containing the antigen a and the antigen B is dropped onto the sample pad 2, and the antigen a and the antigen B move forward to the first and second conjugate pads 301 and 302 due to capillary force, react with the anti-a antibody complex on the first conjugate pad 301, react with the anti-B antibody complex on the second conjugate pad 302, and continue to move forward.
(4) On the first reaction pad 401, the complex of the anti-A antibody complex and the A antigen is combined with the anti-A antibody on the first detection zone 7, the nanoprobe is gathered and developed, and part of the complex continuously moves forwards to the first control zone 8 to be combined with the goat anti-mouse IgG for development; on the second reaction pad 402, the complex of the anti-B antibody complex and the B antigen is combined with the anti-B antibody on the second detection zone 9, the nanoprobe is gathered and developed, part of the complex continuously moves forward to the second control zone 10, is combined with the goat anti-mouse IgG and is developed, and the detection result is judged according to the color of the pigment zone.
Claims (10)
1. The utility model provides a multidirectional joint detection test paper strip, the test paper strip by PVC bottom plate (1), sample pad (2), combination pad (3), reaction pad (4) and absorb water pad (5) constitute, sample pad (2), combination pad (3), reaction pad (4) and absorb water pad (5) arrange its characterized in that in proper order on PVC bottom plate (1):
the PVC base plate (1) is provided with branch base plates by taking the position of the sample pad (2) as the center, the branch base plates radially extend to the periphery, and the number of the branch base plates is 2-10.
2. The multi-directional joint test strip of claim 1, wherein the strip comprises 2 branch substrates, a first branch substrate (101) and a second branch substrate (102); the bonding pad (3) comprises a first bonding pad (301) and a second bonding pad (302); the reaction pad (4) comprises a first reaction pad (401) and a second reaction pad (402); the water absorption pad (5) comprises a first water absorption pad (501) and a second water absorption pad (502);
the sample pad (2), the first combination pad (301), the first reaction pad (401) and the first water absorption pad (501) are sequentially overlapped on the first branch bottom plate (101), the width of the sample pad is equal to that of the first branch bottom plate (101), and the sample pad, the first combination pad, the first reaction pad and the first water absorption pad are arranged to cover the whole first branch bottom plate (101); the sample pad (2), the second combination pad (302), the second reaction pad (402) and the second water absorption pad (502) are sequentially lapped on the second branch bottom plate (102), the width of the sample pad is equal to that of the second branch bottom plate (102), and the sample pad, the second combination pad, the second reaction pad and the second water absorption pad are arranged to cover the whole second branch bottom plate (102); the first branch bottom plate (101) and the second branch bottom plate (102) are connected at the position of the sample pad (2), and an included angle is formed at the connection position and is 10-90 degrees; the first reaction pad (401) is provided with a first detection belt (7) and a first control belt (8), the first detection belt (7) is arranged at one end, close to the first combination pad (301), of the first reaction pad (401), and the first control belt (8) is arranged at one end, close to the first water absorption pad (501), of the first reaction pad (401); the second reaction pad (402) is provided with a second detection belt (9) and a second control belt (10), the second detection belt (9) is arranged at one end, close to the second combination pad (302), of the second reaction pad (402), and the second control belt (10) is arranged at one end, close to the second water absorption pad (502), of the second reaction pad (402).
3. The multi-directional combination test strip of claim 2, wherein the first test strip (7) is coated with an anti-a antibody; the second detection belt (9) is coated with an anti-B antibody; the first control band (8) and the second control band (10) are coated with immunoglobulin.
4. The multi-directional combined test strip of claim 3, wherein the immunoglobulin is derived from an immunoglobulin of antibody-derived species origin.
5. The multi-directional combination test strip of claim 2, wherein the first conjugate pad (301) has an anti-a antibody complex immobilized thereon, and the second conjugate pad (302) has an anti-B antibody complex immobilized thereon.
6. The multi-directional combined test strip of claim 5, wherein the nanoprobes used for the anti-A antibody complex and the anti-B antibody complex are the same nanoprobe or different nanoprobes.
7. The multi-directional combination test strip of claim 5, wherein the anti-A antibody coated on the first test strip (7) is different from the anti-A antibody immobilized on the first conjugate pad (301); the anti-B antibody coated by the second detection belt (9) is different from the anti-B antibody solidified on the second combination pad (302).
8. The multi-directional combination test strip of claim 2, wherein the first test strip (7) and the first control strip (8) are spaced apart by an interval equal to the interval between the second test strip (9) and the second control strip (10) of 2-4 mm.
9. The multi-directional combined test strip of claim 2, wherein the first reaction pad (401) and the second reaction pad (402) are respectively provided with a nitrocellulose membrane with the same size, and the test strip and the control strip are coated on the nitrocellulose membrane.
10. The multi-directional combined test strip of claim 1, wherein the sample pad (2) is connected to the conjugate pad (3) for a length of 1-2mm, the conjugate pad (3) is connected to the reaction pad (4) for a length of 1-2mm, and the reaction pad (4) is connected to the absorbent pad (5) for a length of 1-2 mm.
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| CN201922093794.1U CN211263495U (en) | 2019-11-28 | 2019-11-28 | Multi-directional combined detection test strip |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110736831A (en) * | 2019-11-28 | 2020-01-31 | 河南大学 | multidirectional combined test strip |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110736831A (en) * | 2019-11-28 | 2020-01-31 | 河南大学 | multidirectional combined test strip |
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Effective date of registration: 20220302 Address after: 518000 1601-a, building 1, Yinxing Zhijie, No. 1301-70, sightseeing Road, Xinlan community, Guanlan street, Longhua District, Shenzhen, Guangdong Province Patentee after: Shenzhen Wenwen Biotechnology Co.,Ltd. Address before: 475004 new campus of Henan University, Jinming District, Kaifeng City, Henan Province Patentee before: Henan University |
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