CN103983643A - Kit for rapidly detecting calcium peroxide in wheat flour and detecting method of kit - Google Patents
Kit for rapidly detecting calcium peroxide in wheat flour and detecting method of kit Download PDFInfo
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- CN103983643A CN103983643A CN201410243446.7A CN201410243446A CN103983643A CN 103983643 A CN103983643 A CN 103983643A CN 201410243446 A CN201410243446 A CN 201410243446A CN 103983643 A CN103983643 A CN 103983643A
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Abstract
The invention relates to a kit for rapidly detecting calcium peroxide in wheat flour and a detecting method of the kit. The kit is characterized by being composed of a diluted acid solution, pH test paper, a pH regulator, a sensitizer, a color developing agent and a standard colorimetric tape; the detecting method comprises the following steps of adding a wheat flour sample into a 50mL of triangular flask with a stopper, adding diluted acid, shaking uniformly and standing, regulating pH with the pH regulator, adding the sensitizer and the color developing agent, shaking uniformly and standing, observing color and comparing with the standard colorimetric tape, so as to obtain the rough content range of calcium peroxide in wheat flour. The kit and the detecting method are simple and convenient to operate and are suitable for field inspection.
Description
Technical field
The invention belongs to preparation and the application thereof of food additives quick detection kit, relate to specifically kit and detection method that in a kind of wheat flour, calper calcium peroxide fast qualitative detects.
Background technology
Calper calcium peroxide molecular formula is CaO
2, generally as antiseptic, germifuge, bleaching agent etc.Calper calcium peroxide adds in wheat flour, can when higher degree bleaching wheat flour, improve gluten biceps and dough resistance, and China was once widely used in the past calper calcium peroxide as wheat flour treating agent and specified that its maximum addition is 50 mg/Kg.But research shows recently, the edible wheat flour meeting stimulating gastrointestinal road that contains excessive calper calcium peroxide, initiation is had a stomach upset, and the long-term edible liver metabolism burden that can increase the weight of human body, causes chronic disease.In February, 2011,7 departments such as the Ministry of Public Health combine the file bulletin of having issued " about the bulletin (2011 No. 4) of cancelling food additives benzoyl peroxide, calper calcium peroxide ", have cancelled calper calcium peroxide as wheat flour treating agent.At present, about the detection of calper calcium peroxide in wheat flour, there is no GB recommend method, the detection method that document has been reported has combined analytical method, colourimetry, chemiluminescence detecting method etc., but these methods due to complex operation, required instrument costliness, test the restriction of required environmental baseline, be difficult to carry out at the scene.
Summary of the invention
Object of the present invention is just in order to provide a kind of easy and can realize the quick detection kit of calper calcium peroxide in the wheat flour of Site Detection and the detection method based on this kit.
The object of the invention is to realize by following technique measures:
In wheat flour of the present invention, the quick detection kit of calper calcium peroxide comprises dilute acid soln, pH test paper, pH adjusting agent, sensitizer, developer and standard colorimetric band.
Diluted acid described in the present invention is 0.1mol/L ~ 0.3 mol/L dilute sulfuric acid; PH test paper is accurate pH test paper, and pH value scope is 4.5 ~ 9.0; Described pH value correctives is the sodium hydroxide solution of 0.2 mol/L; Described sensitizer comprises 0.5% horseradish peroxidase, 0.05 mol/L potassium iodide, wherein, horseradish peroxidase 0.52 g that 0.5% horseradish peroxidase solution is 250 U/mg by enzyme activity is dissolved in sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution that 100 mL pH are 6.0 and makes, deposit in brown reagent bottle, keep in Dark Place; Described nitrite ion is 0.05 mol/L quaternary amine [4,4'-(dimethylamino)-diphenyl methane] solution.Described standard colorimetric band is made up of gradual change colour band and graduated scale, and the color of gradual change colour band is deepened successively from right-to-left, the calper calcium peroxide content that graduated scale corresponding to different color depths marks.
The method for making of the band of standard colorimetric described in the present invention is: take some parts of the blank wheat flour of 1.0g ~ 3.0g (not containing any adjuvant), add respectively the calper calcium peroxide titer of variable concentrations, make the content of calper calcium peroxide be respectively 0 mg/Kg ~ 1000 mg/Kg, then add respectively the 0.1mol/L ~ 0.3 mol/L dilute sulfuric acid of 20 mL, shake up rear 5 min of leaving standstill, regulating pH with 0.2 mol/L NaOH is 6.0 left and right, then add respectively the potassium iodide of 1 (approximately 40 μ L) 0.5% horseradish peroxidase and 0.05 mol/L, add again the 0.05 mol/L quaternary amine [4 of 1 mL, 4'-(dimethylamino)-diphenyl methane], shake up rear 5 min of leaving standstill, get supernatant liquor, color after the calper calcium peroxide titer that utilizes scanner to gather variable concentrations develops the color, utilize Photoshop to carry out gradual change matching according to the gradual change rule of color, according to the concentration filling scale of calper calcium peroxide titer, obtain the standard colorimetric band that calper calcium peroxide is measured.
Utilize detection kit of the present invention, detection method is:
(1) get 1.0g ~ 3.0g wheat flour in 50 mL tool plug triangular flasks, add the 0. 1 mol/L ~ 0.3 mol/L dilute sulfuric acids of 20 mL, shake up rear 5 min of leaving standstill;
(2) regulating pH with 0.2 mol/L NaOH is 6.0
(3) add respectively the potassium iodide of 1 (approximately 40 μ L) 0.5% horseradish peroxidase and 0.05 mol/L, then add the 0.05 mol/L quaternary amine [4,4'-(dimethylamino)-diphenyl methane] of 1 mL, shake up rear 5 min of leaving standstill;
(4) observe color and contrast with standard colorimetric band, drawing the roughly content range of calper calcium peroxide in wheat flour.
Step (1) dilute sulfuric acid reacts with the calper calcium peroxide in wheat flour, Hydrogen Peroxide and being present in the stillness of night of upper strata;
The effect that in step (3), the potassium iodide of 0.5% horseradish peroxidase and 0.05 mol/L serves as sensitizer, it adds the sensitivity that can largely promote colour developing, 0.05 mol/L quaternary amine [4,4'-(dimethylamino)-diphenyl methane] serve as developer, under sensitization, the hydrogen peroxide oxidation chromogenic reagent generating in step (1);
beneficial effect of the present invention is as follows:
(1) detection kit of the present invention is easy to carry, and color speed is fast, easy and simple to handle, can realize Site Detection.
(2) Plays of the present invention is more accurate than colour band scale, and its qualitative detection has certain accuracy.
brief description of the drawings:
Fig. 1 is standard colorimetric band of the present invention.
embodiment:
Below in conjunction with embodiment, the invention will be further described
embodiment 1
Detection kit of the present invention comprises dilute acid soln, pH test paper, pH adjusting agent, sensitizer, developer and standard colorimetric band.Wherein pH test paper is accurate pH test paper, and pH value scope is 4.5 ~ 9.0; PH value correctives is the sodium hydroxide solution of 0.2 mol/L; Reaction extraction agent is 0.2 mol/L dilute sulfuric acid; Sensitizer comprises 0.5% horseradish peroxidase, 0.05 mol/L potassium iodide, wherein, horseradish peroxidase 0.52 g that 0.5% horseradish peroxidase solution is 250 U/mg by enzyme activity is dissolved in sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution that 100 mL pH are 6.0 and makes, deposit in brown reagent bottle, keep in Dark Place; Nitrite ion is 0.05 mol/L quaternary amine [4,4'-(dimethylamino)-diphenyl methane] solution; Described standard colorimetric band is made up of gradual change colour band and graduated scale, and the color of gradual change colour band is deepened successively from right-to-left, the calper calcium peroxide content that graduated scale corresponding to different color depths marks.
The method for making of standard colorimetric band is:
Take some parts of the blank wheat flour of 2.0 g (not containing any adjuvant), add respectively the calper calcium peroxide titer of variable concentrations, make the content of calper calcium peroxide be respectively 0 mg/Kg, 10 mg/Kg, 15 mg/Kg, 30 mg/Kg, 40mg/Kg, 50 mg/Kg, 60 mg/Kg, 70 mg/Kg, 100 mg/Kg, 300 mg/Kg, 500 mg/Kg, 700 mg/Kg, 1000 mg/Kg, then add respectively the 0.2 mol/L dilute sulfuric acid of 20 mL, shake up rear 5 min of leaving standstill, regulating pH with 0.2 mol/L NaOH is 6.0 left and right, then add respectively the potassium iodide of 1 (approximately 40 μ L) 0.5% horseradish peroxidase and 0.05 mol/L, add again the 0.05 mol/L quaternary amine [4 of 1 mL, 4'-(dimethylamino)-diphenyl methane], shake up rear 5 min of leaving standstill, get supernatant liquor, color after the calper calcium peroxide titer that utilizes scanner to gather variable concentrations develops the color, utilize Photoshop to carry out gradual change matching according to the gradual change rule of color, according to the concentration filling scale of calper calcium peroxide titer, obtain the standard colorimetric band that calper calcium peroxide is measured.
Utilize the detection method of this detection kit to be:
(1) get 2.0g wheat flour in 50 mL tool plug triangular flasks, add the 0.2 mol/L dilute sulfuric acid of 20 mL, shake up rear 5 min of leaving standstill;
(2) regulating pH with 0.2 mol/L NaOH is 6.0
(3) add respectively the potassium iodide of 1 (approximately 40 μ L) 0.5% horseradish peroxidase and 0.05 mol/L, then add the 0.05 mol/L quaternary amine [4,4'-(dimethylamino)-diphenyl methane] of 1 mL, shake up rear 5 min of leaving standstill;
(4) observe color and contrast with standard colorimetric band, drawing the roughly content range of calper calcium peroxide in wheat flour.
embodiment 2
Utilize detection kit and detection method in embodiment 1, commercially available certain brand steamed bun powder is detected to test, detecting step is as follows:
(1) take 2.0 g brand steamed bun powders in 50 mL tool plug triangular flasks, add the 0.2 mol/L dilute sulfuric acid of 20 mL, shake up rear 5 min of leaving standstill;
(2) regulating pH with 0.2 mol/L NaOH is 6.0 left and right;
(3) add 1 (approximately 40 μ L) 0.5% horseradish peroxidase and liquor kalii iodide of 0.05mol/L, then add 1 mL quaternary amine [4,4'-(dimethylamino)-diphenyl methane] solution, shake up, leave standstill 5 min;
(4) color of sample solution in observation triangular flask, this sample color is faint yellow, illustrates that this sample contains calper calcium peroxide, warp contrasts with the standard colors band in embodiment 1, show that its content is roughly 36 mg/Kg.
Above-described embodiment is only detailed description of the invention; and be not used in restriction the present invention; the technician who it should be pointed out that the industry, not departing within the scope of technology of the present invention and design suitable amendment of the present invention and modification, also should belong to the protection domain of the claims in the present invention.
Claims (5)
1. a quick detection kit for calper calcium peroxide in wheat flour, is characterized in that: described detection kit is made up of dilute acid soln, pH test paper, pH adjusting agent, sensitizer, developer and standard colorimetric band.
2. the preparation method of the inspection of the calper calcium peroxide in wheat flour according to claim 1 quick detection kit, is characterized in that: described diluted acid is 0.1 mol/L ~ 0.3 mol/L dilute sulfuric acid; Described pH test paper is accurate pH test paper; Described pH value correctives is 0.2 mol/L sodium hydroxide solution; Described sensitizer comprises 0.5% horseradish peroxidase, 0.05 mol/L potassium iodide, wherein, horseradish peroxidase 0.52 g that 0.5% horseradish peroxidase solution is 250 U/mg by enzyme activity is dissolved in sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution that 100 mL pH are 6.0 and makes, deposit in brown reagent bottle, keep in Dark Place; Described nitrite ion is 0.05 mol/L quaternary amine [4,4'-(dimethylamino)-diphenyl methane] solution.
3. described in, standard colorimetric band is made up of gradual change colour band and graduated scale, and the color of gradual change colour band is deepened successively from right-to-left, the calper calcium peroxide content that graduated scale corresponding to different color depths marks.
4. calper calcium peroxide quick detection kit in wheat flour according to claim 2, it is characterized in that: wherein the preparation method of standard colorimetric band is: take some parts of the blank wheat flour of 1.0g ~ 3.0g (not containing any adjuvant), add respectively the calper calcium peroxide titer of variable concentrations, make the content of calper calcium peroxide be respectively 0 mg/Kg ~ 1000 mg/Kg, then add respectively the 0. 1mol/L ~ 0.3 mol/L dilute sulfuric acid of 20 mL, shake up rear 5 min of leaving standstill, regulating pH with 0.2 mol/L NaOH is 6.0 left and right, then add respectively the potassium iodide of 1 (approximately 40 μ L) 0.5% horseradish peroxidase and 0.05 mol/L, add again the 0.05 mol/L quaternary amine [4 of 1 mL, 4'-(dimethylamino)-diphenyl methane], shake up rear 5 min of leaving standstill, get supernatant liquor, color after the calper calcium peroxide titer that utilizes scanner to gather variable concentrations develops the color, utilize Photoshop to carry out gradual change matching according to the gradual change rule of color, according to the concentration filling scale of calper calcium peroxide titer, obtain the standard colorimetric band that calper calcium peroxide is measured.
5. the quick detection kit of calper calcium peroxide and a detection method in wheat flour, is characterized in that: described detection method is:
(1) get 1.0g ~ 3.0g wheat flour in 50 mL tool plug triangular flasks, add the 0. 1 mol/L ~ 0.3 mol/L dilute sulfuric acids of 20 mL, shake up rear 5 min of leaving standstill;
(2) regulating pH with 0.2 mol/L NaOH is 6.0;
(3) add respectively the potassium iodide of 1 (approximately 40 μ L) 0.5% horseradish peroxidase and 0.05 mol/L, then add 0.05 mol/L quaternary amine [4,4'-(dimethylamino)-diphenyl methane] solution of 1 mL, shake up rear 5 min of leaving standstill;
(4) observe color and contrast with standard colorimetric band, drawing the content range of calper calcium peroxide in wheat flour.
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| CN201410243446.7A CN103983643A (en) | 2014-06-03 | 2014-06-03 | Kit for rapidly detecting calcium peroxide in wheat flour and detecting method of kit |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106596526A (en) * | 2015-10-20 | 2017-04-26 | 谱尼测试集团江苏有限公司 | Detection method for calcium peroxide content in flour |
| CN109444121A (en) * | 2018-10-16 | 2019-03-08 | 河南工业大学 | The detection kit and detection method of calper calcium peroxide in a kind of flour |
| CN113720791A (en) * | 2021-09-10 | 2021-11-30 | 河南工业大学 | Nano mimic enzyme and preparation method and application thereof |
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| CN102944550A (en) * | 2012-10-15 | 2013-02-27 | 上海谱尼测试技术有限公司 | Detection kit for calcium peroxide in flour and detection method for calcium peroxide |
| CN203101391U (en) * | 2012-11-12 | 2013-07-31 | 南京诺尔曼生物技术有限公司 | Gold-labeled immunochromatography detecting system |
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2014
- 2014-06-03 CN CN201410243446.7A patent/CN103983643A/en active Pending
Patent Citations (5)
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| CN101571490A (en) * | 2008-04-30 | 2009-11-04 | 罗艺斌 | Method for preparing PH test paper standard colorimetric plate and colorimetric plate produced by same |
| CN102901710A (en) * | 2012-09-24 | 2013-01-30 | 谱尼测试科技股份有限公司 | Enzyme-colorimetric method for detecting calcium peroxide in flour |
| CN102944550A (en) * | 2012-10-15 | 2013-02-27 | 上海谱尼测试技术有限公司 | Detection kit for calcium peroxide in flour and detection method for calcium peroxide |
| CN203101391U (en) * | 2012-11-12 | 2013-07-31 | 南京诺尔曼生物技术有限公司 | Gold-labeled immunochromatography detecting system |
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Cited By (3)
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|---|---|---|---|---|
| CN106596526A (en) * | 2015-10-20 | 2017-04-26 | 谱尼测试集团江苏有限公司 | Detection method for calcium peroxide content in flour |
| CN109444121A (en) * | 2018-10-16 | 2019-03-08 | 河南工业大学 | The detection kit and detection method of calper calcium peroxide in a kind of flour |
| CN113720791A (en) * | 2021-09-10 | 2021-11-30 | 河南工业大学 | Nano mimic enzyme and preparation method and application thereof |
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