CN106841512A - A kind of method for detecting VC contents in vitamin C solid beverage - Google Patents
A kind of method for detecting VC contents in vitamin C solid beverage Download PDFInfo
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- CN106841512A CN106841512A CN201611204668.3A CN201611204668A CN106841512A CN 106841512 A CN106841512 A CN 106841512A CN 201611204668 A CN201611204668 A CN 201611204668A CN 106841512 A CN106841512 A CN 106841512A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
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Abstract
A kind of method for being applied in analysis field detect Vitamin C content in vitamin C solid beverage, the detection method comprises the following steps：Testing sample solution is taken, is measured with iodimetry；Add starch indicator, the volume number of record iodine titration solution consumption；Calculate ascorbic content；Described testing sample solution is formulated by solid sample and water, dilute acetic acid solution.The invention source chemicals are easy to get, instrument application is extensive, easy to operate, testing result is accurate and testing cost is low.
Description
Technical field
The detection method of Vitamin C content in a kind of vitamin C solid beverage in the present invention relates to Pharmaceutical Analysis field.
Background technology
Vitamin C also known as ascorbic acid, English name are Vitamin C or Ascorbic acid, are that people and other minorities are raw
The essential nutrients of thing.Many animals can utilize glucose synthetic vitamin C, and human body can not oneself synthetic vitamin C only
Can be obtained from food.Vitamin C be also maximum one kind of daily requirement amount in all vitamins (for by normal adult human,
The nutrient diet of most vitamins recommends supply all below 20 mg/days, and vitamin C be then 100 milligrams/
My god).Vitamin C is generally existed only in plant food, and animal food is substantially free of vitamin C, all kinds of fresh vegetables
With the main source that fruit is her.The detection method that vitamin C solid beverage is used at present is the veterinary antibiotics of GB/T 5009.86
And its DNPH method in the measure of total ascorbic acid in product, the method detection cycle is long, high cost, precision
Difference, therefore, the method for developing Vitamin C content in a kind of vitamin C solid beverage is always new problem urgently to be resolved hurrily.
The content of the invention
It is an object of the invention to provide a kind of detection method of Vitamin C content in vitamin solid beverage, the method
Raw material, reagent are easy to get, and instrument application is extensively, easy to operate, and testing result is accurate, and reduces testing cost.
The object of the present invention is achieved like this：The detection method of Vitamin C content in a kind of vitamin C solid beverage,
The detection method comprises the following steps：
(1) preparation of dilute acetic acid solution：
Glacial acetic acid 60ml is taken, 1000ml is diluted with water to, obtained final product；
(2) preparation of starch indicator solution：
Soluble starch 0.5g is taken, after the 5ml that adds water is stirred evenly, is slowly poured into 100ml boiling water, with adding with stirring, continue to boil
Boiling 2 minutes, lets cool, and inclines and takes supernatant liquor, obtains final product；
(3) preparation of need testing solution：
10 bags of this product is taken, finely ground, precision weighs fine powder at least 40g, wherein 80mg is no less than containing vitamin C, in 250ml tri-
In the bottle of angle, plus the new cold water 100ml for boiling and dilute acetic acid solution 10ml, shaking makes dissolving, used as need testing solution；
(4) assay method：
Need testing solution, plus starch indicator solution 1ml are taken, is titrated with 0.05mol/L iodine titration solutions immediately, to the aobvious blueness of solution
And do not taken off in 30 seconds.It is vitamin C of the 0.05mol/L iodine titration solutions equivalent to 8.806mg per 1ml concentration；
(5) computing formula
In formula：
VSample：Test sample consumes the volume of 0.05mol/L iodine titration solutions, ml；
WFor：The weight of test sample, g；
F：The titer of 0.05mol/L iodine titration solutions；
WAverage loading amount：The average loading amount of vitamin C solid beverage, g；
WLabelled amount：The vitamin C sign content of vitamin C solid beverage, g.
The need testing solution for being used should face uses brand-new.
The detection method for being characterized by it of the invention, its principles of chemistry is：(1) reactional equation that the inventive method is related to
Formula is as follows：
C6H8O6+I2→C6H6O6+2HI
(2) using starch as indicator, and accurate measurement result can be obtained with iodine titration solution, and it is easy to operate,
Determine that terminal is easy.(3) need testing solution is prepared using dilute acetic acid solution, testing result relative deviation is smaller.
In a kind of vitamin solid beverage the detection method of Vitamin C content compared with prior art, with source chemicals
It is easy to get, instrument application is extensive, easy to operate, testing result is accurate and the low advantage of testing cost, medicine point will be widely used in
In analysis field.
With reference to embodiment, the present invention is described in detail.
Specific embodiment
Following instance will be helpful to understanding of the invention, but these examples to the present invention only for being illustrated, this hair
It is bright to be not limited to these contents.
Embodiment one
1. need testing solution is prepared：10 bags of this product is taken, finely ground, precision weighs fine powder 41.1648g, 41.2356g, puts respectively
In 250ml triangular flasks, plus the new cold water 100ml for boiling and dilute acetic acid solution 10ml, shaking makes dissolving, used as need testing solution.
Detection method
2. assay method：
Need testing solution, plus starch indicator solution 1ml are taken, is titrated with iodine titration solution (0.05mol/L) immediately, to the aobvious indigo plant of solution
Color did not took off simultaneously in 30 seconds.
3. titration results：
Consumption iodine titration solution (0.05mol/L) volume be：9.36ml、9.42ml
4. result of calculation
In formula：
VSample：The volume of test sample consumption iodine titration solution (0.05mol/L)；
WFor：The weight of test sample, g；
F：The titer of iodine titration solution (0.05mol/L).
WAverage loading amount：The average loading amount of vitamin C solid beverage；
WLabelled amount：The vitamin C sign content of vitamin C solid beverage.
Test sample 1：
Test sample 2：
RSD：0.23%
The selection of the solvent of embodiment two
1. need testing solution is prepared：10 bags of this product is taken, finely ground, precision weighs fine powder 41.3070g, 41.5603g, puts respectively
In 250ml triangular flasks, plus the new cold water 100ml for boiling and dilution heat of sulfuric acid 25ml, shaking makes dissolving, used as need testing solution.
Detection method
2. assay method：
Need testing solution, plus starch indicator solution 1ml are taken, is titrated with iodine titration solution (0.05mol/L) immediately, to the aobvious indigo plant of solution
Color did not took off simultaneously in 30 seconds.
3. titration results：
Consumption iodine titration solution (0.05mol/L) volume be：9.10ml、9.36ml
4. result of calculation
In formula：
VSample：The volume of test sample consumption iodine titration solution (0.05mol/L)；
WFor：The weight of test sample, g；
F：The titer of iodine titration solution (0.05mol/L).
WAverage loading amount：The average loading amount of vitamin C solid beverage；
WLabelled amount：The vitamin C sign content of vitamin C solid beverage.
Test sample 3：
Test sample 4：
RSD：1.10%
Solvent changes dilute sulfuric acid by spirit of vinegar, and titration end-point color hop is not obvious, and causing RSD not meet titration operation will
Ask.
The selection of the sample weighting amount of embodiment three
1. need testing solution is prepared：10 bags of this product is taken, finely ground, precision weighs fine powder 31.4271g, 31.7904g,
20.3256g, 20.5788g put in 250ml triangular flasks respectively, plus the new cold water 100ml for boiling and dilute acetic acid solution 10ml, shaking
Make dissolving, as need testing solution.Detection method
2. assay method：
Need testing solution, plus starch indicator solution 1ml are taken, is titrated with iodine titration solution (0.05mol/L) immediately, to the aobvious indigo plant of solution
Color did not took off simultaneously in 30 seconds.
3. titration results：
Consumption iodine titration solution (0.05mol/L) volume be：6.76ml, 6.99ml,
4. result of calculation
In formula：
VSample：The volume of test sample consumption iodine titration solution (0.05mol/L)；
WFor：The weight of test sample, g；
F：The titer of iodine titration solution (0.05mol/L).
WAverage loading amount：The average loading amount of vitamin C solid beverage；
WLabelled amount：The vitamin C sign content of vitamin C solid beverage.
Test sample 5：
Test sample 6：
RSD：1.10%
Test sample 7：
Test sample 8：
RSD：1.41%
Sample weighting amount is changed into 30g, 20g from 40g, and titration end-point color hop is not obvious, and causing RSD not meet titration operation will
Ask.
Claims (2)
1. in a kind of vitamin C solid beverage Vitamin C content detection method, it is characterised in that：The detection method is included such as
Lower step：
(1) preparation of dilute acetic acid solution：
Glacial acetic acid 60ml is taken, 1000ml is diluted with water to, obtained final product；
(2) preparation of starch indicator solution：
Soluble starch 0.5g is taken, after the 5ml that adds water is stirred evenly, is slowly poured into 100ml boiling water, with adding with stirring, continue to boil 2 points
Clock, lets cool, and inclines and takes supernatant liquor, obtains final product；
(3) preparation of need testing solution：
10 bags of this product is taken, finely ground, precision weighs fine powder at least 40g, wherein 80mg is no less than containing vitamin C, in 250ml triangular flasks
In, plus the new cold water 100ml for boiling and dilute acetic acid solution 10ml, shaking makes dissolving, used as need testing solution；
(4) assay method：
Take need testing solution, plus starch indicator solution 1ml, titrated with 0.05mol/L iodine titration solutions immediately, to solution it is aobvious blue and
Do not taken off in 30 seconds；It is vitamin C of the 0.05mol/L iodine titration solutions equivalent to 8.806mg per 1ml concentration；
(5) computing formula
In formula：
VSample：Test sample consumes the volume of 0.05mol/L iodine titration solutions, ml；
WFor：The weight of test sample, g；
F：The titer of 0.05mol/L iodine titration solutions；
WAverage loading amount：The average loading amount of vitamin C solid beverage, g；
WLabelled amount：The vitamin C sign content of vitamin C solid beverage, g.
2. in a kind of vitamin C solid beverage according to claim 1 Vitamin C content detection method, its feature exists
In：The need testing solution for being used should face uses brand-new.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611204668.3A CN106841512A (en) | 2016-12-23 | 2016-12-23 | A kind of method for detecting VC contents in vitamin C solid beverage |
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| CN201611204668.3A CN106841512A (en) | 2016-12-23 | 2016-12-23 | A kind of method for detecting VC contents in vitamin C solid beverage |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110988256A (en) * | 2019-12-30 | 2020-04-10 | 江苏艾兰得营养品有限公司 | EDTA titration method for rapidly determining Zn content in vitamin C + zinc sustained release tablets |
| CN111380998A (en) * | 2018-12-27 | 2020-07-07 | 江苏艾兰得营养品有限公司 | Iodine titration method for rapidly determining VC content in vitamin C sustained-release tablets |
| CN113671095A (en) * | 2021-08-20 | 2021-11-19 | 江苏艾兰得营养品有限公司 | Titration method of vitamin C in solid preparation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1435690A (en) * | 2002-09-06 | 2003-08-13 | 天津商学院 | Measuring ascorbic acid by iodine differential process |
| CN103091449A (en) * | 2012-11-13 | 2013-05-08 | 江苏艾兰得营养品有限公司 | Method for identifying vitamin C content in vitamin/mineral health product |
-
2016
- 2016-12-23 CN CN201611204668.3A patent/CN106841512A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1435690A (en) * | 2002-09-06 | 2003-08-13 | 天津商学院 | Measuring ascorbic acid by iodine differential process |
| CN103091449A (en) * | 2012-11-13 | 2013-05-08 | 江苏艾兰得营养品有限公司 | Method for identifying vitamin C content in vitamin/mineral health product |
Non-Patent Citations (2)
| Title |
|---|
| 国家药典委员会: "《中华人民共和国药典2010年版二部》", 31 January 2010, 中国医药科技出版社 * |
| 李家庆: "《药物分析技术》", 31 May 2011, 中国医药科技出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111380998A (en) * | 2018-12-27 | 2020-07-07 | 江苏艾兰得营养品有限公司 | Iodine titration method for rapidly determining VC content in vitamin C sustained-release tablets |
| CN110988256A (en) * | 2019-12-30 | 2020-04-10 | 江苏艾兰得营养品有限公司 | EDTA titration method for rapidly determining Zn content in vitamin C + zinc sustained release tablets |
| CN113671095A (en) * | 2021-08-20 | 2021-11-19 | 江苏艾兰得营养品有限公司 | Titration method of vitamin C in solid preparation |
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Application publication date: 20170613 |