CN106353304A - Method and color card for rapid detection of nitrate in food and kit for detecting nitrate in food - Google Patents
Method and color card for rapid detection of nitrate in food and kit for detecting nitrate in food Download PDFInfo
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- CN106353304A CN106353304A CN201610653440.6A CN201610653440A CN106353304A CN 106353304 A CN106353304 A CN 106353304A CN 201610653440 A CN201610653440 A CN 201610653440A CN 106353304 A CN106353304 A CN 106353304A
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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Abstract
The invention provides a method for rapid detection of nitrate in food. The method includes the detection steps of 1), preparing sodium nitrate water solutions sequentially changing in concentration as standard solutions, adding a powder reagent mixed by citric acid, anhydrous sodium sulfate and N-(1-naphthyl) ethylenediamine dihydrochloride, mixing evenly and standing so as to obtain standard chromophoric solutions with the known nitrate content and a certain color gradient; 2), adding purified water into a sample prior to vibrating extraction so as to obtain sample treatment liquid; 3), adding the powder reagent mixed by the citric acid, the anhydrous sodium sulfate and the N-(1-naphthyl) ethylenediamine dihydrochloride according to a specific ratio into the sample treatment liquid, mixing evenly and standing so as to obtain a sample chromophoric solution with color; 4), comparing the color of the sample chromophoric solution with the color of every standard chromophoric solution so as to obtain the content of the nitrate in the detected sample. The method for rapid detection of the nitrate in the food is capable of detecting the content of the nitrate in the food rapidly, simply and conveniently and high in sensitivity, thereby being suitable for non-specialized persons to perform on-site rapid determination.
Description
Technical field
The present invention relates to a kind of food analyses inspection technology field, more particularly, to a kind of based on nitre in the food of chemical reaction
The method for quick skill detection colour atla of hydrochlorate and test kit.
Background technology
In the safety detection of modern food, nitrate Clinical significance of detecting is particularly significant.Nitrate be safe from harm in itself but
Nitrite can be reduced into, under certain condition, nitrite and protein can be formed by force in the presence of people's internal microorganism
Strong carcinogenic nitroso-compound.Serious harm health.Research shows that in human body 80% nitrate comes from vegetable
Dish, and China is a large amount of every year, excess or unreasonably using chemical fertilizer especially nitrogenous fertilizer on crops, cause nitric acid in fruit and vegerable
Salt pollution problem is quite serious, particularly leaf vegetables, and this causes serious harm to the food service industry of China.Research with
New quick, easy, the safe nitrate detection method of exploitation is significant for the monitoring of intermediate links food.
At present, the method measuring nitrate has cadmium pole process, enzyme process, chromatography etc..Cadmium pole process is the country of detection nitrate
Standard method.This method must operate in the lab, and complex operation, time-consuming longer；Cadmium can volatilize, and human body is had very
Big toxicity.Enzyme process agents useful for same is difficult to store, and is not suitable for the Site Detection operation outside laboratory.Chromatography instrument is held high
Expensive, and reagent consumption is larger, needs professional testing staff just operable it is impossible to popularize again.
A kind of nitrate quick detection agent (patent No.: 201310577915.4) be adapted to detect for nitrate content 0.01～
Medium between 0.5mg/l, and minimum in pollutants in food limit standard (gb 2762-2012) limit the quantity as mineral water
Middle 45mg/l, infant food is then up to 100mg/kg, and this nitrate quick detection agent then cannot detect that nitrate content is higher
Sample.
Content of the invention
Present invention offer is a kind of can easy, quickly mensure Nitrate Content in Food content method.The inventive method has
Have reagent dosage few, quick, easy the features such as, be suitable to the field quick detection of layman, be available for the administration for industry and commerce, the Ministry of Agriculture
Door and Department of Qulity Supervision scene carry out rapid semi-quantitative detection to Nitrate Content in Food, greatly reduce the workload of test in laboratory, according to
Method timely Maintenance Market order.
For this first aspect present invention provide a kind of can side that is easy, quickly measuring Nitrate Content in Food content
Method.
A kind of method for quick of Nitrate Content in Food, comprises the following steps:
1) prepare the sodium nitrate aqueous solution that some concentration change successively, as standard solution, take variable concentrations above-mentioned standard
Solution, adds nitrate detectable, and described nitrate detectable is that citric acid, anhydrous sodium sulfate and hydrochloride naphthodiamide mix
The powder reagent closing, stands after mixing, obtains known nitrate content and have the standard coloration solution of certain color gradient；
2) add pure water in the sample, mechanical shaking extraction obtains sample treatment liquid；
3) take above-mentioned sample treatment liquid, add nitrate detectable, stand after mixing, obtain having coloured sample and show
Color solution；
4) color of the color of sample chromophoric solution and each standard coloration solution is contrasted, drawn nitre in test sample
The content of hydrochlorate.
Preferably, described step 1) described in each standard solution concentration be respectively every kg solution in 0mg containing sodium nitrate,
50mg、100mg、200mg、300mg、400mg、500mg、1000mg.
Preferably, step 2) in the mass ratio of sample and pure water be 1:5～20.
Preferably, described step 1) and 3) described in the citric acid of nitrate detectable, anhydrous sodium sulfate and hydrochloric acid naphthalene
The mass ratio of ethylenediamine is: 9:10:3.
It is furthermore preferred that described step 1) and 3) in nitrate detectable also include manganese sulfate monohydrate, p-aminobenzene sulfonic acid
And zinc powder, the quality proportioning of each component is as follows:
Citric acid: 9 parts
Anhydrous sodium sulfate: 10 parts
Manganese sulfate monohydrate: 2.5 parts
P-aminobenzene sulfonic acid: 1 part
Hydrochloride naphthodiamide: 0.6 part
Zinc powder: 3 parts.
Step 1) and step 3) described in nitrate detectable proportioning as follows: citric acid: 18g, anhydrous sodium sulfate:
20g, manganese sulfate monohydrate: 5g, p-aminobenzene sulfonic acid: 2g, hydrochloride naphthodiamide: 1.2g, zinc powder: 6g, above-mentioned chemical reagent used:
Citric acid, anhydrous sodium sulfate, manganese sulfate monohydrate, p-aminobenzene sulfonic acid, hydrochloride naphthodiamide, zinc powder are analysis pure chemicals,
And granular reagent answer grind into powder after carry out proportioning.
In addition present invention also offers the quick detection colour atla of Nitrate Content in Food and its using method.
A kind of solid colour of the quick detection colour atla of Nitrate Content in Food, colour atla color and standard coloration solution.Described
Standard coloration solution be by 0mg containing sodium nitrate in concentration respectively every kg solution, 50mg, 100mg, 200mg, 300mg, 400mg,
The sodium nitrate aqueous solution of 500mg, 1000mg, as standard solution, takes variable concentrations above-mentioned standard solution, adds the matter of each component
Amount proportioning is: citric acid: 9 parts；Anhydrous sodium sulfate: 10 parts；Manganese sulfate monohydrate: 2.5 parts；P-aminobenzene sulfonic acid: 1 part；Hydrochloric acid naphthalene
Ethylenediamine: 0.6 part；Zinc powder: 3 parts of powder reagent, stand after mixing, the known nitrate content obtaining simultaneously has certain color ladder
The standard coloration solution of degree.
The using method of quick detection colour atla, comprises the steps:
(1) add pure water in the sample, mechanical shaking extraction obtains sample treatment liquid；
(2) take above-mentioned sample treatment liquid, add nitrate detectable, stand after mixing, obtain having coloured sample
Chromophoric solution；
(3) color of the color of sample chromophoric solution and quick detection colour atla is contrasted, drawn nitre in test sample
The content of hydrochlorate.
Method according to a first aspect of the present invention, further includes: the color of each standard coloration solution is fabricated to colour atla,
The color of sample chromophoric solution is compared with colour atla again, the content of nitrate in sample can be drawn.This measure can be omitted
Detection will prepare the repeat step of nitrate standard coloration solution so that the detection of whole nitrate is convenient, fast every time
Prompt.
Based on the method for first aspect present invention, second aspect present invention provides a kind of detection kit, and it is used for examining
Survey Nitrate Content in Food content, described test kit include box body and:
A) nitrate detectable, it is packaged in reagent bottle；Described nitrate detectable is citric acid, anhydrous slufuric acid
Sodium, the powder reagent of hydrochloride naphthodiamide mixing are it is preferable that described nitrate detectable is citric acid for quality proportioning: 9 parts；
Anhydrous sodium sulfate: 10 parts；Manganese sulfate monohydrate: 2.5 parts；P-aminobenzene sulfonic acid: 1 part；Hydrochloride naphthodiamide: 0.6 part；Zinc powder: 3 parts
Powder reagent.
B) operation instruction data, records the operational approach detecting Nitrate Content in Food content using this test kit thereon.
Test kit according to a second aspect of the present invention, wherein said reagent bottle is can repeatedly open and seal light tight
Reagent bottle or valve bag.Material for encapsulating the reagent bottle of mentioned reagent can not do special restriction, for example, can be
With bottle stopper, the vial of bottle cap, plastic bottle or valve bag etc..
Test kit according to a second aspect of the present invention, wherein said operation instruction data can be operation instructions, detection
Method explanation, detection criterion etc., its form can be to be printed with paper, card of relevant information etc., this operation instruction
Data substantially describes the method described in first aspect present invention.Therefore, in first aspect present invention any embodiment, its
In arbitrary technical characteristic be equally applicable to the test kit of second aspect present invention or be applied to the other side of the present invention.
Test kit according to a second aspect of the present invention, can be widely used for the related functional departments such as bureau of agriculture, food officina quick
Nitrate in detection food, can also pass through ordinary people family, and common people do it yourself to detect, really realize the peace on the tip of the tongue
Entirely.
Specific embodiment
With reference to specific embodiment, the present invention is elaborated.
Further illustrate the present invention below by an instantiation, it should be understood, however, that, these examples are only to use
It is used in specifically describing in more detail, and be not to be construed as any form and limit the present invention.Embodiment one, making nitrate
Detectable
Nitrate detectable is the powder reagent of citric acid, anhydrous sodium sulfate and hydrochloride naphthodiamide mixing, citric acid,
The mass ratio of anhydrous sodium sulfate and hydrochloride naphthodiamide is: 9:10:3.
Embodiment two, making nitrate detectable
Nitrate detectable also includes manganese sulfate monohydrate, p-aminobenzene sulfonic acid and zinc powder, and the quality proportioning of each component is such as
Under:
Citric acid: 9 parts
Anhydrous sodium sulfate: 10 parts
Manganese sulfate monohydrate: 2.5 parts
P-aminobenzene sulfonic acid: 1 part
Hydrochloride naphthodiamide: 0.6 part
Zinc powder: 3 parts.
Embodiment three, making standard color card
Prepare many parts of standard solution, each standard solution is the aqueous solution of sodium nitrate, the concentration of its sodium nitrate is respectively every kg
Sodium nitrate 0mg, 2.5mg, 5mg, 10mg, 20mg, 30mg, 40mg, 50mg, 100mg is contained in solution；Take each standard solution 1ml
In 10ml color comparison tube, each standard solution adds an earpick (about 0.025g) nitrate detectable, after mixing
Standing 1 minute, obtains each standard coloration solution of known nitrate concentration.
The color of each standard coloration solution is processed by computer and is fabricated to standard color card, the corresponding nitre of each color on colour atla
The content of hydrochlorate be respectively 0mg/kg, 25mg/kg, 50mg/kg, 100mg/kg, 200mg/kg, 300mg/kg, 400mg/kg,
500mg/kg、1000mg/kg.
After above-mentioned standard colour atla completes, can be with life-time service.
Example IV, prepare sample chromophoric solution
Weigh 1g sample in 10ml graded tube, plus pure water be to graduation mark, shaking, standing, take supernatant 1ml in
In 1.5ml centrifuge tube, add an earpick nitrate detectable, mix, stand 1 minute, obtain final product sample chromophoric solution.
Embodiment five, the content of detection Nitrate Content in Food
The sample preparing chromophoric solution and standard color card are compared, by estimating judgement sample chromophoric solution
Which color in color and colour atla is closer to thus draw the substantially content of tested Nitrate Content in Food.
Specific embodiment described herein is only explanation for example to present invention spirit.The affiliated technology of the present invention is led
The technical staff in domain can be made various modifications or supplement or replaced using similar mode to described specific embodiment
Generation, but the spirit without departing from the present invention or surmount scope defined in appended claims.
Claims (10)
1. a kind of method for quick of Nitrate Content in Food, comprises the following steps:
1) prepare the sodium nitrate aqueous solution that some concentration change successively, as standard solution, take variable concentrations above-mentioned standard solution,
Add nitrate detectable, described nitrate detectable is citric acid, anhydrous sodium sulfate and hydrochloride naphthodiamide mixing
Powder reagent, stands after mixing, obtains known nitrate content and have the standard coloration solution of certain color gradient；
2) add pure water in the sample, mechanical shaking extraction obtains sample treatment liquid；
3) take above-mentioned sample treatment liquid, add nitrate detectable, stand after mixing, obtain having coloured sample colour developing molten
Liquid；
4) color of the color of sample chromophoric solution and each standard coloration solution is contrasted, drawn nitrate in test sample
Content.
2. nitrate according to claim 1 method for quick it is characterised in that: described step 1) described in respectively mark
The concentration of quasi- solution be respectively every kg solution in 0mg containing sodium nitrate, 50mg, 100mg, 200mg, 300mg, 400mg, 500mg,
1000mg.
3. method according to claim 1 is it is characterised in that further include: step 2) in sample and pure water quality
Ratio is 1:5～20.
4. nitrate according to claim 1 method for quick it is characterised in that: described step 1) and 3) described in
The mass ratio of the citric acid of nitrate detectable, anhydrous sodium sulfate and hydrochloride naphthodiamide is: 9:10:3.
5. nitrate according to claim 4 method for quick it is characterised in that: described step 1) and 3) described in
Nitrate detectable also include manganese sulfate monohydrate, p-aminobenzene sulfonic acid and zinc powder,
The quality proportioning of each component is as follows:
Citric acid: 9 parts
Anhydrous sodium sulfate: 10 parts
Manganese sulfate monohydrate: 2.5 parts
P-aminobenzene sulfonic acid: 1 part
Hydrochloride naphthodiamide: 0.6 part
Zinc powder: 3 parts.
6. a kind of Nitrate Content in Food quick detection colour atla it is characterised in that: colour atla color is arbitrary with claim 1-5 described
Step 1 in method) standard coloration solution solid colour.
7. quick detection colour atla according to claim 6 it is characterised in that: described standard coloration solution is by concentration respectively
Water-soluble for the sodium nitrate of 0mg containing sodium nitrate in every kg solution, 50mg, 100mg, 200mg, 300mg, 400mg, 500mg, 1000mg
Liquid, as standard solution, takes variable concentrations above-mentioned standard solution, the quality proportioning of each component of addition is: citric acid: 9 parts；Anhydrous
Sodium sulfate: 10 parts；Manganese sulfate monohydrate: 2.5 parts；P-aminobenzene sulfonic acid: 1 part；Hydrochloride naphthodiamide: 0.6 part；Zinc powder: 3 parts of powder
Last reagent, standing, the known nitrate content obtaining the standard coloration solution having certain color gradient after mixing.
8. quick detection colour atla described in claim 6 or 7 using method it is characterised in that:
(1) add pure water in the sample, mechanical shaking extraction obtains sample treatment liquid；
(2) take above-mentioned sample treatment liquid, add nitrate detectable, stand after mixing, obtain having coloured sample colour developing
Solution；
(3) color of the color of sample chromophoric solution and quick detection colour atla is contrasted, drawn nitrate in test sample
Content.
9. a kind of detection kit of Nitrate Content in Food it is characterised in that: described test kit include box body and:
A) nitrate detectable, it is packaged in reagent bottle；Described nitrate detectable be citric acid, anhydrous sodium sulfate,
The powder reagent of hydrochloride naphthodiamide mixing；
B) operation instruction data, records the operational approach detecting Nitrate Content in Food content using this test kit thereon.
10. according to claim 9 a kind of detection kit of Nitrate Content in Food it is characterised in that: the inspection of described nitrate
Test agent is citric acid for quality proportioning: 9 parts；Anhydrous sodium sulfate: 10 parts；Manganese sulfate monohydrate: 2.5 parts；P-aminobenzene sulfonic acid: 1
Part；Hydrochloride naphthodiamide: 0.6 part；Zinc powder: 3 parts of powder reagent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610653440.6A CN106353304A (en) | 2016-08-10 | 2016-08-10 | Method and color card for rapid detection of nitrate in food and kit for detecting nitrate in food |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610653440.6A CN106353304A (en) | 2016-08-10 | 2016-08-10 | Method and color card for rapid detection of nitrate in food and kit for detecting nitrate in food |
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| CN106353304A true CN106353304A (en) | 2017-01-25 |
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| CN201610653440.6A Pending CN106353304A (en) | 2016-08-10 | 2016-08-10 | Method and color card for rapid detection of nitrate in food and kit for detecting nitrate in food |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107764815A (en) * | 2017-09-29 | 2018-03-06 | 郑州欧柯奇仪器制造有限公司 | The method of quick detection Nitrate Content in Food |
| CN109827921A (en) * | 2019-03-12 | 2019-05-31 | 闫宏涛 | A kind of NO3-N and NO2-N assay analoids |
| CN109856123A (en) * | 2018-12-11 | 2019-06-07 | 广东环凯微生物科技有限公司 | A kind of nitrate quick detection reagent and detection method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107764815A (en) * | 2017-09-29 | 2018-03-06 | 郑州欧柯奇仪器制造有限公司 | The method of quick detection Nitrate Content in Food |
| CN109856123A (en) * | 2018-12-11 | 2019-06-07 | 广东环凯微生物科技有限公司 | A kind of nitrate quick detection reagent and detection method |
| CN109827921A (en) * | 2019-03-12 | 2019-05-31 | 闫宏涛 | A kind of NO3-N and NO2-N assay analoids |
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