CN105115965A - Soft drink synthetic pigment fast detection method and kit - Google Patents
Soft drink synthetic pigment fast detection method and kit Download PDFInfo
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- CN105115965A CN105115965A CN201510447152.0A CN201510447152A CN105115965A CN 105115965 A CN105115965 A CN 105115965A CN 201510447152 A CN201510447152 A CN 201510447152A CN 105115965 A CN105115965 A CN 105115965A
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Abstract
The invention discloses a synthetic pigment fast detection method and a preparation method of a detection kit. The synthetic pigment fast detection method comprises adjusting sample pH, putting a fast detection card into a sample, carrying out shaking for 5min so that the fast detection card and a synthetic pigment in the sample are fully bonded, taking out the fast detection card, carrying out natural airing, and observing a color of the fast detection card to determine if the sample contains the synthetic pigment and determine a content range. The pigment fast detection card contains an adsorbent and can be processed to form various specifications, and according to sample pigment content, the detection card with corresponding specification is selected. The detection method is free of complex detection equipment and apparatus, can be operated simply, has a low cost, short detection time, good detection sensitivity and accurate result, and can be used for on-site fast detection and sample screening. The soft drink synthetic pigment fast detection method and kit have the advantages of no toxicity and no damage to the environment.
Description
Technical field
The present invention relates to method that synthetic dyestuff in soft drink detects fast and speed surveys box.
Background technology
Synthetic dyestuff has physical property and stablizes, cheap, easy coloring and extensively by the favor of food production producer, a large amount of synthetic dyestuff that uses has become a kind of normality in soft drink, on market, many illegal businessmans are misused in the illegal activities such as food, or pretend to claim to be natural colouring matter.Synthetic dyestuff in soft drink mainly contains that amaranth, famille rose, lemon yellow, newly red, erythrosine, temptation are red, sunset yellow etc.These synthetic dyestuff long-term low dose are taken in, and there is carcinogenic, teratogenesis, mutagenic possibility, strict must control its use in soft drink.The method of current these synthetic dyestuffs of detection is: paper chromatography, spectrophotometric method, high performance liquid chromatography, thin-layered chromatography, oscilloscopic polarography etc.
These methods detect accurately, but detection time is long, and process is complicated, and needs professional and technical personnel to participate in, expensive, is not easy to Site Detection.
Summary of the invention
Described in background technology, problem existing for classic method is detected to synthetic dyestuff in food in order to solve, the present invention designs a kind of method for quick and speed surveys box, reach easy and simple to handle, highly sensitive, low cost, safety and environmental protection, for carrying out fast qualitative and quantitatively detecting to synthetic dyestuff in soft drink.
The technical solution used in the present invention is as follows:
Synthetic dyestuff method for quick in soft drink, after regulating testing sample pH value to 3-5 with acid-base modifier, pigment quick measuring card is placed in testing sample, after shake well reaction 5-10min, take out pigment quick measuring card, naturally dry under room temperature, observe the color of pigment quick measuring card, according to color change, whether containing synthetic dyestuff in judgement sample; Described pigment quick measuring card is made up of with the polyamide material be sprayed on solid phase matrix the solid phase matrix of acid and alkali-resistance.
Described synthetic dyestuff is newly red, amaranth, erythrosine, famille rose, lemon yellow, sunset yellow or light blue.
In order to reach better technique effect:
Pigment quick measuring card and standard color comparison card are carried out color comparison, semiquantitative determination can be carried out to the content of synthetic dyestuff in sample.
Described acid-base modifier, contains acid regulator and alkaline conditioner two kinds.Described acid-base modifier: acid-base modifier is 0.2-0.5g/ml citric acid solution, alkaline conditioner is concentration 0.2-0.5g/ml sodium hydroxide solution;
The polyamide thickness of spraying is 0.05-0.1mm, and polyamide material is nylon 6 or nylon66 fiber, preferably selects nylon 6.Synthetic dyestuff quick detection kit in soft drink, is made up of acid-base modifier, pigment quick measuring card and standard color comparison card:
Described acid-base modifier: acid-base modifier is 0.2-0.5g/ml citric acid solution; Alkaline conditioner is concentration 0.2-0.5g/ml sodium hydroxide solution;
Described pigment quick measuring card: be made up of solid phase matrix and the polyamide be sprayed on solid phase matrix of acid and alkali-resistance, preferably polyamide membrane thickness is 0.05-0.1mm;
Described standard color comparison card: preparation is containing normal concentration synthetic dyestuff aqueous solution, by acid-base modifier adjust ph to 3-5, pigment quick measuring card is placed in one, after shake well reaction 5-10min, take out pigment quick measuring card, naturally dry under room temperature, obtain the standard color comparison card of this normal concentration synthetic dyestuff.The standard color comparison card of various criterion concentration synthetic dyestuff can be made with this.
By polyamide material, synthetic dyestuff is adsorbed, then wash away impurity, then result is differentiated.
In order to keep the stability of quick measuring card and prevent humidity, preferably adopt single packing.
Jolting when conveniently measuring, pigment quick measuring card goes back jug, preferably plastic handles.
Beneficial effect of the present invention:
This product is the method for quick to synthetic dyestuff in soft drink.Use the synthetic dyestuff in this Product checking soft drink, need not reagent preparation in addition, easy and simple to handle, do not need professional to carry out.Speed is surveyed in box and is not corroded pungency medicine containing poisonous and harmful, can not work the mischief to environment.This Product checking time, detection time was short within 10min, high specificity, and result is easy to judge, is suitable for field quick detection and grass-roots work.
Accompanying drawing explanation
Fig. 1 is standard color comparison card exemplary plot
Embodiment
Embodiment 1 pigment quick measuring card
In soft drink, synthetic dyestuff quick detection kit is made up of acid-base modifier, pigment quick measuring card and standard color comparison card;
The solid substance of pigment quick measuring card is the positive chip card be made up of the synthetic plastic of acid and alkali-resistance, diameter 10mm.Solid substance sprays polyamide material (nylon 6), thickness is 0.1mm; Pigment quick measuring card goes back jug;
Wherein acid-base modifier: acid-base modifier is 0.2-0.5g/ml citric acid solution, alkaline conditioner is concentration 0.2-0.5g/ml sodium hydroxide solution;
Standard color comparison card: preparation is containing normal concentration synthetic dyestuff aqueous solution, by acid-base modifier adjust ph to 3-5, pigment quick measuring card is placed in one, after shake well reaction (or vortex oscillation) 5-10min, take out pigment quick measuring card, naturally dry under room temperature, obtain the standard color comparison card of this normal concentration synthetic dyestuff.Fig. 1 is the standard color comparison card of several pigment.
Detect:
Fruit juice is shaken up gently, gets 20ml and be placed in sample hose.Regulate testing sample pH value to 3-5 with acid-base modifier.Getting pigment quick measuring card is immersed in sample, and jolting is taken out after 5 ~ 8 minutes with pure water hydro-peening card.Dry under natural conditions, if quick measuring card has color, illustrate that samples of juice contains synthetic dyestuff as newly red, amaranth, erythrosine, famille rose, lemon yellow, sunset yellow or light blue; If without color, interpret sample is not containing synthetic dyestuff.According to the color of pigment quick measuring card, carry out colorimetric with standard color comparison card, can the content of synthetic dyestuff in interpretation samples of juice.
Embodiment 2: quick measuring card detection mode compares
Relatively quick measuring card detection mode (jolting and not jolting) is for the impact of pigment adsorption effect.
Prepare the lemon yellow of 1.0mg/L, sunset yellow and carmine three kinds of pigment standard solutions respectively.Get 20ml respectively and be placed in sample hose (2 pipes got respectively by often kind of pigment standard items), by acid-base modifier adjust ph to 3-5.Pigment quick measuring card is put into sample hose, a wherein pipe shake well 5-10min of often kind of pigment standard items, another manages not jolting, takes out quick measuring card pure water hydro-peening card, dries under natural conditions.Result shows, jolting detection mode is obviously better than not jolting (table 1).
There are jolting and the testing result without jolting
Note: "-" expression does not detect, "+" expression detects, and "+" more multilist shows more obvious relative to color when only having.
Embodiment: 3: quick measuring card method compares with national standard method (thin-layered chromatography and high performance liquid chromatography)
Gather the fruit juice of commercially available 10 kinds of different brands, adopt method described in the method for the invention (embodiment 1) and standard GB/T/T5009.35-2003 to detect respectively.Result is as shown in table 2, and the result of the method for the invention is consistent with the testing result of national standard method.
The commercially available fruit juice testing result of table 2 compares
Note: "-" expression does not detect, "+" expression detects.
The determination of embodiment 4: three kinds of synthetic dyestuff quick measuring card method lowest detectable limit
Compound concentration is the lemon yellow of 0.1mg/L, 0.2mg/L, 0.3mg/L, 0.4mg/L, 0.5mg/L, 1.0mg/L, 2.0mg/L, 3.0mg/L, 4.0mg/L, sunset yellow and carmine three kinds of pigment standard solutions respectively.Detect with according to jolting mode in embodiment 2, according to the color of colour developing card, the lowest detection obtaining lemon yellow, sunset yellow and famille rose is limited to 0.3mg/L, 0.4mg/L, 0.4mg/L, three kinds of maximum additions of synthetic dyestuff far below standard GB/T 2760-2011 regulation: lemon yellow 0.1g/kg, sunset yellow 0.1g/kg, carmine 0.05g/kg.
Claims (8)
1. synthetic dyestuff method for quick in a soft drink, it is characterized in that: after regulating testing sample pH value to 3-5 with acid-base modifier, pigment quick measuring card is placed in testing sample, after shake well reaction 5-10min, take out pigment quick measuring card, naturally dry under room temperature, observe the color of pigment quick measuring card, if any in color then sample whether containing synthetic dyestuff, as without in color then sample without synthetic dyestuff; Described pigment quick measuring card is made up of with the polyamide material be sprayed on solid phase matrix the solid phase matrix of acid and alkali-resistance, and described polyamide is nylon 6 or nylon66 fiber.
2. synthetic dyestuff method for quick in soft drink according to claim 1, is characterized in that: it is characterized in that described synthetic dyestuff is for newly red, amaranth, erythrosine, famille rose, lemon yellow, sunset yellow or light blue.
3. synthetic dyestuff method for quick in soft drink according to claim 1, is characterized in that: pigment quick measuring card and standard color comparison card are carried out color comparison, can carry out semiquantitative determination to the content of synthetic dyestuff in sample.
4. synthetic dyestuff method for quick in soft drink according to claim 1, is characterized in that: described acid-base modifier, contains acid regulator and alkaline conditioner two kinds.
5. synthetic dyestuff method for quick in soft drink according to claim 4, is characterized in that: described acid-base modifier: acid-base modifier is 0.2-0.5g/ml citric acid solution, and alkaline conditioner is concentration 0.2-0.5g/ml sodium hydroxide solution.
6. synthetic dyestuff method for quick in soft drink according to claim 1, is characterized in that: the polyamide material thickness of spraying is 0.05-0.1mm; Polyamide material is nylon 6 or nylon66 fiber, is preferably nylon 6.
7. synthetic dyestuff quick detection kit in soft drink, is characterized in that: be made up of acid-base modifier, pigment quick measuring card and standard color comparison card;
Wherein acid-base modifier: acid-base modifier is 0.2-0.5g/ml citric acid solution, alkaline conditioner is concentration 0.2-0.5g/ml sodium hydroxide solution;
Described pigment quick measuring card: be made up of solid phase matrix and the polyamide material be sprayed on solid phase matrix of acid and alkali-resistance, preferably polyamide material film thickness is 0.05-0.1mm;
Described standard color comparison card: preparation is containing normal concentration synthetic dyestuff aqueous solution, by acid-base modifier adjust ph to 3-5, pigment quick measuring card is placed in one, after shake well reaction 5-10min, take out pigment quick measuring card, naturally dry under room temperature, obtain the standard color comparison card of this normal concentration synthetic dyestuff.
8. synthetic dyestuff quick detection kit in soft drink according to claim 7, is characterized in that: pigment quick measuring card goes back jug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510447152.0A CN105115965A (en) | 2015-07-27 | 2015-07-27 | Soft drink synthetic pigment fast detection method and kit |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510447152.0A CN105115965A (en) | 2015-07-27 | 2015-07-27 | Soft drink synthetic pigment fast detection method and kit |
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| CN105115965A true CN105115965A (en) | 2015-12-02 |
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| CN201510447152.0A Pending CN105115965A (en) | 2015-07-27 | 2015-07-27 | Soft drink synthetic pigment fast detection method and kit |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107703129A (en) * | 2017-09-08 | 2018-02-16 | 瓮福达州化工有限责任公司 | A kind of detection method of colouring agent aberration |
| CN113828283A (en) * | 2021-09-24 | 2021-12-24 | 安徽工业大学 | Beverage pigment visual measurement colorimetric card, preparation method and application thereof |
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| JP2003294626A (en) * | 2002-03-29 | 2003-10-15 | Nisshin Seifun Group Inc | Method for analyzing tissue construction of multi- components dispersion food |
| CN101339174A (en) * | 2008-08-11 | 2009-01-07 | 浙江永宁药业股份有限公司 | Method for determining carthamus tinctorius yellow color content |
| WO2009029964A2 (en) * | 2007-09-03 | 2009-03-12 | Schalkhammer Thomas, Mag | Sensory pigments used on food, packaging, paper and pharmaceutical and electronic products |
| CN101644681A (en) * | 2008-08-05 | 2010-02-10 | 潘华 | Method for quickly detecting food synthetic pigment and detection box of food synthetic pigment |
| CN103091314A (en) * | 2013-01-16 | 2013-05-08 | 深圳市计量质量检测研究院 | Rapid detection method for water-soluble azo dyes in foods |
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2015
- 2015-07-27 CN CN201510447152.0A patent/CN105115965A/en active Pending
Patent Citations (5)
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|---|---|---|---|---|
| JP2003294626A (en) * | 2002-03-29 | 2003-10-15 | Nisshin Seifun Group Inc | Method for analyzing tissue construction of multi- components dispersion food |
| WO2009029964A2 (en) * | 2007-09-03 | 2009-03-12 | Schalkhammer Thomas, Mag | Sensory pigments used on food, packaging, paper and pharmaceutical and electronic products |
| CN101644681A (en) * | 2008-08-05 | 2010-02-10 | 潘华 | Method for quickly detecting food synthetic pigment and detection box of food synthetic pigment |
| CN101339174A (en) * | 2008-08-11 | 2009-01-07 | 浙江永宁药业股份有限公司 | Method for determining carthamus tinctorius yellow color content |
| CN103091314A (en) * | 2013-01-16 | 2013-05-08 | 深圳市计量质量检测研究院 | Rapid detection method for water-soluble azo dyes in foods |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107703129A (en) * | 2017-09-08 | 2018-02-16 | 瓮福达州化工有限责任公司 | A kind of detection method of colouring agent aberration |
| CN113828283A (en) * | 2021-09-24 | 2021-12-24 | 安徽工业大学 | Beverage pigment visual measurement colorimetric card, preparation method and application thereof |
| CN113828283B (en) * | 2021-09-24 | 2023-10-31 | 安徽工业大学 | Visual beverage pigment color comparison card, preparation method and application thereof |
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