CN103217419A - Quick detecting method for formaldehyde content in aquatic product - Google Patents
Quick detecting method for formaldehyde content in aquatic product Download PDFInfo
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- CN103217419A CN103217419A CN2013101320799A CN201310132079A CN103217419A CN 103217419 A CN103217419 A CN 103217419A CN 2013101320799 A CN2013101320799 A CN 2013101320799A CN 201310132079 A CN201310132079 A CN 201310132079A CN 103217419 A CN103217419 A CN 103217419A
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Abstract
The invention discloses a quick detecting method for formaldehyde content in an aquatic product. The quick detecting method comprises the following steps: (1) preparing a Schiff reagent; (2) making a standard color chart, namely respectively adding 5mL formaldehyde solutions with different concentrations to 8 different colorimetric tubes, respectively adding a 0.5mL Schiff reagent to the colorimetric tubes, adjusting the pH of the solutions to 2.5, shaking up the solutions, stewing and developing for 10 minutes, and making the color chart by taking colors displayed by the solutions as standard colors; and (3) detecting, namely extracting a 5mL soak solution of an aquatic product sample to be detected, adding a 0.5mL Schiff reagent to the soak solution, adjusting the pH of the solution to 2.5, shaking up the solution, stewing and developing for 10 minutes, and comparing the color displayed by the solution with standard colorimetric splines. The quick detecting method disclosed by the invention has the characteristic that the formaldehyde and the Schiff reagent react at a normal state to generate a purple-red compound, and the shade of the compound is in direct proportion to formaldehyde content so as to realize colorimetric assay. The quick detecting method has the advantages of simple and quick operation, high sensitivity, simple conditions, low reagent price, and the like.
Description
Technical field
The present invention relates to a kind of aquatic products detection method, be specifically related to the method for content of formaldehyde in a kind of fast detecting aquatic products.
Background technology
Formaldehyde is the simplest carbonyls, is the colourless gas that the specific stimulation smell is arranged under normal conditions, and as low grade aldehyde, it can dissolve each other with water, its aqueous solution of 35%～40% common name formalin.Formaldehyde is noxious material, and by its toxicity grading, formaldehyde belongs to the moderate toxicity material, and the people once wrongly takes 1020 μ L formalins, can cause death.Acceptable intake every day of USEPA suggestion is no more than 0.2mg/ ton body weight/day.But coup injury people's oral cavity, throat, esophagus and stomach lining after generation formaldehyde acute oral is poisoned, produce toxic reaction simultaneously, the lighter's dizziness, cough, vomiting, Upper abdominal pain, stupor, shock, pulmonary edema, hepatic renal dysfunction appear in weight person, cause hemorrhage, kidney failure and respiratory failure and death.Experimental study shows that formaldehyde also has teratogenesis, carcinogenesis.Genetoxic discovers that formaldehyde can cause gene mutation and chromosome damage.Country prohibites and add formaldehyde in food, and formaldehyde must not list in (all aquatic products) project that detects in the Ministry of Agriculture in the industry standard of the NY5073-2001 " poisonous and harmful substance is limited the quantity of in the pollution-free food aquatic products " of September 30 calendar year 2001 issue.Owing to formaldehyde has effect anticorrosion, that finalize the design, bleach, some illegal retailers soak aquatic products with formalin and sell in order to reach anticorrosion, fresh-keeping, the goodlooking purpose of aquatic products, reap staggering profits.This way serious harm consumer healthy.In order to ensure consumer's legitimate rights and interests, the detection of formaldehyde in food is just seemed extremely important.
The method of measuring formaldehyde in food content at present is a lot, wherein mainly contain spectrophotometric method, vapor-phase chromatography, high performance liquid chromatography, oscilloscopic polarography etc., wherein spectrophotometric method is to measure the common analysis of formaldehyde, it mainly includes acetylacetone method, variable color acid system, paramagenta method, phenol reagent process and 4-amino-3 hydrazine-5 sulfydryl-1,2,4-three azepines penta (AHMT) etc.These methods analysts are accurate, are applicable to accurate analyzing and testing under the laboratory condition, owing to most large-scale precision instruments that use are not suitable for the field control check and analysis.Used poisonous mercuric chloride reagent owing to acetylacetone method in addition, and colour developing is slow, needs heating to urge its reaction and finish; The variable color acid system need boil under concentrated sulphuric acid condition, and NO is arranged
2 -, aldehydes and phenols disturb; The colour developing product instability of phenylhydrazine hydrochloride method is subject to the influence of turbidity, iron, organic acid, pure and mild phenols.The principle that this paper utilizes Schiff's reagent and formaldehyde reaction to produce a kind of aubergine compound has been set up the method for quick that a kind of aquatic products and water are sent out content of formaldehyde in the product.Experimental result shows whether this method can exceed standard to removing residue formaldehyde in the commercially available aquatic products fast more accurately and makes quick judgement, to guarantee people's edible safety.Related work does not have bibliographical information.That method has is simple to operate, quick, accurate, phenomenon is obvious, cheap and the commercialized degree advantages of higher of reagent is fit to the administration for industry and commerce, food hygiene regulator, supermarket and family and commercially available aquatic products and water are sent out whether is contained formaldehyde in the product and do fast detecting and judge.
Though Chinese patent CN200510020511.0 discloses the method for formaldehyde in a kind of fast detecting water-soaked food and the beverage, comprises the steps: 1) the pH value of tested sample solution is transferred to 9.0; 2) on color comparison tube or white test paper, respectively drip tested sample solution and 1.0mol/L sodium sulfite solution; 3) be the indicator test that develops the color with 1g/L thymolphthalein ethanolic solution then; 4) show blue depth qualitative detection content of formaldehyde scope according to color comparison tube or white test paper wetted portions: during qualitative analysis, concentration of formaldehyde is greater than 5ppm in solution, the formaldehyde absolute magnitude is during greater than 0.25 μ g, and colorimetric liquid in pipe or white test paper wetted portions just can demonstrate blueness; 5) further determine content of formaldehyde, adopt miniature pH meter to carry out quantitative test.The advantage of this method is highly sensitive, and the detection method cost is low, and is pollution-free, easy quicklook, and detection method is easy, quick, effectively avoids or eliminate the problem of background value.But because operating process is comparatively complicated, cost is higher, has also limited its applying in real life.
Summary of the invention
The colorimetric card and the reagent pipe that the object of the present invention is to provide a kind of method for quick of aquatic products content of formaldehyde and be used for this method, it is higher to solve existing Determination of formaldehyde content method detectable cost, problems such as operation inconvenience.
For solving the problems of the technologies described above, technical scheme of the present invention is: a kind of method for quick of aquatic products content of formaldehyde comprises the steps: 1) the preparation Schiff's reagent; 2) production standard colorimetric card: add respectively in 8 different color comparison tubes that concentration is respectively 0,2.0,5.0,10.0,20.0,40.0,60.0, the formalin 5mL of 100.0mg/kg, add the 0.5mL Schiff's reagent more respectively, regulator solution pH value is 2.5, leave standstill after shaking up the colour developing 10min, with solution apparent color make colour atla as the benchmark color of standard color comparison card; 3) detect: get aquatic products sample soak solution 5mL to be measured, add the 0.5mL Schiff's reagent, regulator solution pH is 2.5, leaves standstill colour developing 10min after shaking up, compare with the solution apparent color of institute and with standard colorimetric batten, can judge the content range that whether contains formaldehyde and formaldehyde in the aquatic products to be measured.
As preferably, the preparation method of the Schiff's reagent in the described step 1) is: the pinkish red hydrochloride of 0.2g is dissolved in the distilled water of 100mL heat, treats that solution cooling back adds 2g NaHSO
3, adding the 2mL concentrated hydrochloric acid then slowly, solution presents colourless or light yellow, this solution is diluted to 200mL with redistilled water, promptly.
The detectable pipe that is used for the method for quick of aquatic products content of formaldehyde, described reagent pipe is a 10mL sealing color comparison tube, described sealing color comparison tube contains the 0.5mL Schiff's reagent, and pH value of solution is 2.5, and described sealing color comparison tube surface is provided with the solution scale mark of a 5mL.
The method for making of standard color comparison card that is used for the method for quick of aquatic products content of formaldehyde: add respectively at 8 different color comparison tubes that concentration is respectively 0,2.0,5.0,10.0,20.0,40.0,60.0, the formalin 5mL of 100.0mg/kg, add the 0.5mL Schiff's reagent more respectively, regulator solution pH value is 2.5, leave standstill colour developing 10min after shaking up, with solution apparent color as the benchmark color of standard color comparison card, and take pictures with digital camera, photo is made into colour atla.
Adopted technique scheme, beneficial effect of the present invention is: the principle of utilizing Schiff's reagent and formaldehyde reaction to produce a kind of aubergine compound has been set up the method for quick that a kind of aquatic products and water are sent out content of formaldehyde in the product.Experimental result shows whether this method can exceed standard to removing residue formaldehyde in the commercially available aquatic products fast more accurately and makes quick judgement, to guarantee people's edible safety.Related work does not have bibliographical information.That method has is simple to operate, quick, accurate, phenomenon is obvious, cheap and the commercialized degree advantages of higher of reagent is fit to the administration for industry and commerce, food hygiene regulator, supermarket and family and commercially available aquatic products and water are sent out whether is contained formaldehyde in the product and do fast detecting and judge.
Embodiment
The present invention is further described below in conjunction with embodiment.
Embodiment: preparation Schiff's reagent: the pinkish red hydrochloride of 0.2g is dissolved in the distilled water of 100mL heat, treats that solution cooling back adds 2g NaHSO
3(AR), add the 2mL concentrated hydrochloric acid then slowly, solution presents colourless or light yellow, and this solution is diluted to 200mL with redistilled water, puts into 4 ℃ of refrigerators and refrigerates standby.
Preparation 100mg/kg formaldehyde standard solution: 40% the formalin of accurately measuring 1.0mL with the 2mL transfer pipet is carried out constant volume with distilled water in the 50mL volumetric flask, shake up.Accurately measure this solution of 6.25mL in the 500mL volumetric flask with the 10mL transfer pipet again, carry out constant volume, shake up, put into 4 ℃ of refrigerators and refrigerate standby with redistilled water.
Under optimum reaction condition, the formalin that adds the 5mL variable concentrations in 8 color-comparison tubes respectively, make that its concentration is respectively 0,2.0,5.0,10.0,20.0,40.0,60.0,100.0mg/kg, add the 0.5mL Schiff's reagent more respectively, regulator solution pH value is 2.5, leaves standstill colour developing 10min after shaking up, with the benchmark color of this color as standard color comparison card, and take pictures with digital camera, photo is made into colour atla, the basis for estimation of content of formaldehyde during as fast detecting formaldehyde.
Gather respectively 3 parts of tripe soak solution, sea-tangle leacheate, peeled shrimp soak solutions, gather respectively 3 parts of jellyfish silk soak solution, tripe soak solution, squid soak solutions.Get each sample soak solution 5mL, add the 0.5mL Schiff's reagent respectively, regulator solution pH is 2.5, leaves standstill colour developing 10min after shaking up, and the result is as follows in colour developing:
Through comparing the overall recall rate of method of the present invention and its basically identical with formaldehyde qualitative detection result.
For further facilitating fast detecting, pack into behind the 4mol/mL HCl solution mixing with 0.5mL Schiff's reagent and 0.5mL in the sealing color comparison tube of 10mL, highly locate to do a groove at test tube 5mL, only need directly the soak solution of determinand to be added to the groove place of color comparison tube during use, shake up, leave standstill colour developing 10min then, contrast according to the apparent color of solution institute and standard color comparison card and can judge the content range that whether contains formaldehyde and formaldehyde in the measured matter.
The present invention is not limited to above-mentioned concrete embodiment, and those of ordinary skill in the art is from above-mentioned design, and without performing creative labour, all conversion of having done all drop within protection scope of the present invention.
Claims (4)
1. the method for quick of an aquatic products content of formaldehyde is characterized in that comprising the steps: 1) the preparation Schiff's reagent; 2) production standard colorimetric card: add respectively in 8 different color comparison tubes that concentration is respectively 0,2.0,5.0,10.0,20.0,40.0,60.0, the formalin 5mL of 100.0mg/kg, add the 0.5mL Schiff's reagent more respectively, regulator solution pH value is 2.5, leave standstill after shaking up the colour developing 10min, with solution apparent color make colour atla as the benchmark color of standard color comparison card; 3) detect: get aquatic products sample soak solution 5mL to be measured, add the 0.5mL Schiff's reagent, regulator solution pH is 2.5, leaves standstill colour developing 10min after shaking up, solution apparent color of institute and standard colorimetric batten are compared, can judge the content range that whether contains formaldehyde and formaldehyde in the aquatic products to be measured.
2. the method for quick of aquatic products content of formaldehyde according to claim 1, it is characterized in that, the preparation method of the Schiff's reagent in the described step 1) is: the pinkish red hydrochloride of 0.2g is dissolved in the distilled water of 100mL heat, treats that solution cooling back adds 2g NaHSO
3, adding the 2mL concentrated hydrochloric acid then slowly, solution presents colourless or light yellow, this solution is diluted to 200mL with redistilled water, promptly.
3. detectable pipe that is used for the method for quick of the described aquatic products content of formaldehyde of claim 1, it is characterized in that, described reagent pipe is a 10mL sealing color comparison tube, described sealing color comparison tube contains the 0.5mL Schiff's reagent, pH value of solution is 2.5, and described sealing color comparison tube surface is provided with the solution scale mark of a 5mL.
4. standard color comparison card that is used for the method for quick of the described aquatic products content of formaldehyde of claim 1, it is characterized in that, the method for making of described standard color comparison card is: add respectively in 8 different color comparison tubes that concentration is respectively 0,2.0,5.0,10.0,20.0,40.0,60.0, the formalin 5mL of 100.0mg/kg, add the 0.5mL Schiff's reagent more respectively, regulator solution pH value is 2.5, leave standstill after shaking up the colour developing 10min, with solution apparent color make colour atla as the benchmark color of standard color comparison card.
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| CN2013101320799A CN103217419A (en) | 2013-04-08 | 2013-04-08 | Quick detecting method for formaldehyde content in aquatic product |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105388153A (en) * | 2015-11-30 | 2016-03-09 | 湖北微控生物科技有限公司 | Rapid qualitative analysis method of formaldehyde in glutaraldehyde |
| CN105445260A (en) * | 2015-11-16 | 2016-03-30 | 渤海大学 | Detection method for formaldehyde content in Spanish mackerel |
| CN107505312A (en) * | 2017-05-10 | 2017-12-22 | 惠安爱馨机电设备科技有限公司 | A kind of air quality detector |
| CN110053119A (en) * | 2019-05-28 | 2019-07-26 | 江苏莱宝装饰材料有限公司 | A kind of manufacturing method of flame-retardant board |
| CN111044509A (en) * | 2019-12-24 | 2020-04-21 | 北京建工环境修复股份有限公司 | Method for quickly identifying chlorobenzene pollutants in soil and evaluating concentration of chlorobenzene pollutants |
| CN111044508A (en) * | 2019-12-24 | 2020-04-21 | 北京建工环境修复股份有限公司 | Method for rapidly evaluating pollution level of chlorobenzene substances in water body |
| CN114166835A (en) * | 2021-12-08 | 2022-03-11 | 桂林医学院 | Method for analyzing drug content in health product by tracing auxiliary agent |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105445260A (en) * | 2015-11-16 | 2016-03-30 | 渤海大学 | Detection method for formaldehyde content in Spanish mackerel |
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| CN107505312A (en) * | 2017-05-10 | 2017-12-22 | 惠安爱馨机电设备科技有限公司 | A kind of air quality detector |
| CN110053119A (en) * | 2019-05-28 | 2019-07-26 | 江苏莱宝装饰材料有限公司 | A kind of manufacturing method of flame-retardant board |
| CN111044509A (en) * | 2019-12-24 | 2020-04-21 | 北京建工环境修复股份有限公司 | Method for quickly identifying chlorobenzene pollutants in soil and evaluating concentration of chlorobenzene pollutants |
| CN111044508A (en) * | 2019-12-24 | 2020-04-21 | 北京建工环境修复股份有限公司 | Method for rapidly evaluating pollution level of chlorobenzene substances in water body |
| CN114166835A (en) * | 2021-12-08 | 2022-03-11 | 桂林医学院 | Method for analyzing drug content in health product by tracing auxiliary agent |
| CN114166835B (en) * | 2021-12-08 | 2024-02-20 | 桂林医学院 | Method for analyzing drug content in health care product by tracing auxiliary agent |
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Application publication date: 20130724 |