CN101644681A - Method for quickly detecting food synthetic pigment and detection box of food synthetic pigment - Google Patents
Method for quickly detecting food synthetic pigment and detection box of food synthetic pigment Download PDFInfo
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- CN101644681A CN101644681A CN200810029995A CN200810029995A CN101644681A CN 101644681 A CN101644681 A CN 101644681A CN 200810029995 A CN200810029995 A CN 200810029995A CN 200810029995 A CN200810029995 A CN 200810029995A CN 101644681 A CN101644681 A CN 101644681A
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- pigment
- thin slice
- synthetic
- colour developing
- acid
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000001040 synthetic pigment Substances 0.000 title claims abstract description 20
- 235000013305 food Nutrition 0.000 title claims description 23
- 238000001514 detection method Methods 0.000 title abstract description 15
- 239000000049 pigment Substances 0.000 claims abstract description 33
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 25
- 235000011114 ammonium hydroxide Nutrition 0.000 claims abstract description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 5
- 239000012530 fluid Substances 0.000 claims abstract description 3
- 238000001179 sorption measurement Methods 0.000 claims abstract 6
- 238000006243 chemical reaction Methods 0.000 claims abstract 3
- 238000012360 testing method Methods 0.000 claims description 37
- 239000000975 dye Substances 0.000 claims description 20
- 238000010521 absorption reaction Methods 0.000 claims description 19
- 239000002253 acid Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 11
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 8
- 241001232787 Epiphragma Species 0.000 claims description 7
- 239000003607 modifier Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 210000002268 wool Anatomy 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 238000004040 coloring Methods 0.000 claims description 4
- 239000012535 impurity Substances 0.000 claims description 4
- 239000004952 Polyamide Substances 0.000 claims description 3
- 229920002647 polyamide Polymers 0.000 claims description 3
- 239000012445 acidic reagent Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- 239000003513 alkali Substances 0.000 abstract description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 abstract 2
- 239000011259 mixed solution Substances 0.000 abstract 1
- 231100000252 nontoxic Toxicity 0.000 abstract 1
- 230000003000 nontoxic effect Effects 0.000 abstract 1
- 239000003381 stabilizer Substances 0.000 abstract 1
- 239000002585 base Substances 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000009940 knitting Methods 0.000 description 2
- 229920002994 synthetic fiber Polymers 0.000 description 2
- 244000248349 Citrus limon Species 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 235000000177 Indigofera tinctoria Nutrition 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229920002522 Wood fibre Polymers 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 235000012730 carminic acid Nutrition 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 235000019601 food appearance Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229940097275 indigo Drugs 0.000 description 1
- COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000004816 paper chromatography Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002025 wood fiber Substances 0.000 description 1
Images
Abstract
The invention discloses a method for quickly detecting a synthetic pigment, which comprises the following steps: performing adsorption reaction on a sample treatment fluid and an adsorption flake of apigment detection card; dropping a synthetic pigment color developing reagent on the adsorption flake; folding the detection card to ensure that the adsorption flake is contacted and overlapped witha color developing flake; opening the pigment detection card to observe the color of the color developing flake; and judging whether a sample contains the synthetic pigment according to color changes.The pigment detection card consists of the adsorption flake containing a spreader and the color developing flake containing an acid-alkali stabilizer, and the two flakes are independent respectivelyand can be connected with each other under a mutually contact-less condition; and the synthetic pigment color developing reagent is a mixed solution of ammonia water and ethanol, wherein the volume ratio of the ammonia water to the ethanol is 1:5-15. Compared with the prior detection method, the method has the advantages of standardizing the detection process, and having simple and convenient operation, short detection time, sensitive reaction and accurate detection result, which is the ideal on-site detection method for basic staffs. Moreover, the method is nontoxic and harmless and cannot cause pollution to the environment.
Description
(1) technical field
The present invention relates to a kind of food synthetic pigment method for quick and food synthetic pigment and detect box.
(2) background technology
The color and luster of food is one of key factor of food appearance, and some food producers are driven by economic interests, adds harmful synthetic dyestuff in food, as lemon yellow, carmine and indigo etc.The method that detects these pigments at present is: under acid condition, polyamide or steeping wool can combine with water-soluble acid pigment, again can desorption under alkali condition, the pigment of resolving is separated with paper chromatography or thin-layered chromatography, with standard relatively, but just qualitative, quantitative.Owing to there is not the product of a whole set of moulding, the testing staff needs own elder generation that knitting wool is carried out the degreasing processing usually, and sample also will carry out pre-treatment, removes alcohol, vibration removing carbon dioxide and removes starch and protein etc. as heating.Need use a bit of knitting wool during detection, it is very inconvenient to operate, and each one gimmick difference, and whole process also can't standardization, and therefore, repeatability, reliability and the accuracy to the result impacts easily.
This method complex operation, complexity, time-consuming, effort need the technical skill personnel operation, are unfavorable for that the mensuration of on-the-spot a large amount of samples and basic staff use.And use too much soda acid and organic solvent easily environment to be polluted, therefore this method can't reach the effect that detects safely and fast at all, can not carry out supervision and management timely and effectively to violation food production enterprise.
(3) summary of the invention
1, technical matters to be solved by this invention:
Described in the background technology synthetic dyestuff in the food is detected the existing problem of classic method in order to solve, design a kind of standardized method for quick and detect the box product, easy and simple to handle, highly sensitive, the result is accurate, cost is low, safety and environmental protection, practical fast, be used for that the food synthetic dyestuff is carried out fast qualitative and detect the object of the invention that Here it is.
2, the technical solution used in the present invention:
The thin slice that the pigment test card is made by two different materials among the present invention constitutes, and wherein adsorbs thin slice and is made by wool or polyamide material, and handle through special spreader-sticker; The colour developing thin slice is made by natural wood fiber material, contains the ph stability agent; Two kinds of thin slices can be distinguished separately, also can link to each other under mutually non-touching situation.
For the stability of the thin slice that keeps developing the color with prevent humidity, be preferably on the colour developing thin slice and add one deck epiphragma.
In order to carry absorption thin slice and colour developing thin slice, preferably they are fixed on the base plate paper with glue, base plate paper is preferably made by the synthetic material of acid and alkali-resistance.
Described acid-base modifier has comprised two kinds of acid regulator and alkaline conditioners.Wherein, acid regulator is the solution of acid reagent preparation, and its concentration is 0.5-3.0mol/L; Alkaline conditioner is the solution of alkaline reagent preparation, and its concentration is 0.5-3.0mol/L.
Described synthetic dyestuff developer is the mixed liquor of ammoniacal liquor and ethanol, wherein, and the volume ratio of ammoniacal liquor and ethanol=1: 5-15.
A kind of food synthetic pigment detects box, comprising:
Acid-base modifier: comprising concentration of hydrochloric acid is that 0.5-3.0mol/L acid regulator and naoh concentration are the 0.5-3.0mol/L alkaline conditioner;
The pigment test card;
The synthetic dyestuff developer: synthetic dyestuff developer volume ratio is volume ratio=1 of ammoniacal liquor and ethanol: 5-15;
PH test paper.
It is as follows that described food synthetic pigment detects the box using method:
(1) after solid sample shreds, take by weighing 10 and restrain in 50 ml beakers, add 30 milliliters of pure water, soak after 10 minutes, the sample thief soak solution is in another beaker, and is to be measured; Fluid sample is directly got 30 milliliters in beaker, and is to be measured;
(2) with the pH value of pH test paper test sample liquid or sample soak solution,, can directly detect if between 3-5.If greater than 5, or, then drip acid-base modifier to pH 3-5 less than 3;
(3) get a slice pigment test card and will post absorption one of thin slice and insert in the above-mentioned sample liquid, swing gently and allowed its abundant adsorpting pigment in two minutes;
(4) test card that will be adsorbed with pigment forwards flush away impurity and unnecessary pigment in the pure water to;
(5) drip the synthetic dyestuff developer to the absorption thin slice, reacted for 10 seconds, throw off the transparent epiphragma on the colour developing thin slice, with the test card doubling, it is overlapping to allow absorption thin slice and colour developing thin slice be in contact with one another, and opens test card, and observing the colour developing thin slice has non-pigment to adhere to, natural colouring matter can not adhere to, and the synthetic dyestuff that is that adheres to is arranged.
3, beneficial effect of the present invention:
This product is the method for quick to synthetic dyestuff in the food.Need not to join in addition reagent when detecting with this novel product, easy and simple to handle, need not the professional, be easy to apply.Do not contain deep-etching pungency medicine in the testing product, can not pollute environment.And this novel product is short detection time, is quick on the draw, and measurement result is accurate, is easy to observe colorimetric, is the best approach that on-the-spot detection and basic staff use.
(4) accompanying drawing and embodiment
Description of drawings
Accompanying drawing 1 is the synoptic diagram that detects a kind of pattern of pigment test card in the box for food synthetic pigment.
Accompanying drawing 2 is longitudinal sectional views of present embodiment.With reference to accompanying drawing, present embodiment is made up of following each several part
1---the base plate paper of two kinds of thin slices of representative carrying
2---representative colour developing thin slice
3---representative absorption thin slice
4---represent epiphragma
Embodiment
The pigment test card
Base plate paper is to be made by the synthetic material of acid and alkali-resistance, and its length * wide size is 60 millimeters * 16 millimeters; The colour developing thin slice is made by the natural xylogen that contains the ph stability agent, and its diameter is 10 millimeters; The absorption thin slice is to be made by the fleece material that contains spreader-sticker, and its diameter is 12 millimeters; Two thin slices are fixed on the base plate paper with viscose glue, and after the doubling of base plate paper, two thin slices are in contact with one another; Be stamped the film of being made by the PET compound substance on the colour developing thin slice, its length * wide size is 30 millimeters * 16 millimeters.
The detection of synthetic dyestuff (1) in the beverage
Get 30 ml sample liquid in beaker; With the pH value of pH test paper test sample liquid,, can directly detect if between 3-5.If greater than 5, the hydrochloric acid that then drips 0.5mol/L is to pH 3-5; If less than 3, the NaOH that then drips 0.5mol/L is to pH 3-5.Get a slice pigment test card and will post absorption one of thin slice and insert in the sample liquid, swing gently and allowed its abundant adsorpting pigment in two minutes; Forward the test card that is adsorbed with pigment in the pure water flush away impurity and unnecessary pigment; The mixed liquor that drips two ammoniacal liquor and ethanol reacted for 10 seconds to adsorbing on the thin slice, wherein the volume ratio of ammoniacal liquor and ethanol=1: 15; Throw off the transparent epiphragma on the colour developing thin slice, with the test card doubling, it is overlapping to allow absorption thin slice and colour developing thin slice be in contact with one another, and opens test card, and observing the colour developing thin slice has non-pigment to adhere to, and natural colouring matter can not adhere to, and the synthetic dyestuff that is that adheres to is arranged.
The detection of synthetic dyestuff (2) in the beverage
Get 30 ml sample liquid in beaker; With the pH value of pH test paper test sample liquid,, can directly detect if between 3-5.If greater than 5, the hydrochloric acid that then drips 1.5mol/L is to pH 3-5; If less than 3, the NaOH that then drips 1.5mol/L is to pH 3-5.Get a slice pigment test card and will post absorption one of thin slice and insert in the sample liquid, swing gently and allowed its abundant adsorpting pigment in two minutes; Forward the test card that is adsorbed with pigment in the pure water flush away impurity and unnecessary pigment; The mixed liquor that drips two ammoniacal liquor and ethanol reacted for 10 seconds to adsorbing on the thin slice, wherein the volume ratio of ammoniacal liquor and ethanol=1: 8; Throw off the transparent epiphragma on the colour developing thin slice, with the test card doubling, it is overlapping to allow absorption thin slice and colour developing thin slice be in contact with one another, and opens test card, and observing the colour developing thin slice has non-pigment to adhere to, and natural colouring matter can not adhere to, and the synthetic dyestuff that is that adheres to is arranged.
Claims (7)
1, a kind of food synthetic pigment method for quick, it is characterized in that: regulate the pH value of sample with acid-base modifier after, absorption chip sorption reaction with sample preparation liquid and pigment test card, on the absorption thin slice, drip the synthetic dyestuff developer, the doubling test card, it is overlapping to allow absorption thin slice and colour developing thin slice be in contact with one another, and opens the color that the pigment test card is observed the colour developing thin slice, according to change color, whether contain synthetic dyestuff in the judgement sample.
Described pigment test card is to be made of absorption thin slice that contains spreader-sticker and the colour developing thin slice that contains the ph stability agent, and two thin slices can be distinguished separately, also can link to each other under mutually non-touching situation; Described synthetic dyestuff developer is the mixed liquor of ammoniacal liquor and ethanol, wherein, and the volume ratio of ammoniacal liquor and ethanol=1: 5-15.
2, food synthetic pigment method for quick as claimed in claim 1 is characterized in that: described acid-base modifier has comprised two kinds of acid regulator and alkaline conditioners.Wherein, acid regulator is the solution of acid reagent preparation, and its concentration is 0.5-3.0mol/L; Alkaline conditioner is the solution of alkaline reagent preparation, and its concentration is 0.5-3.0mol/L.
3, food synthetic pigment method for quick as claimed in claim 1 is characterized in that: described absorption thin slice is to be made by wool that contains spreader-sticker or polyamide material, and described colour developing thin slice is made by the natural xylogen that contains the ph stability agent.
4, as claim 1 or 3 described food synthetic pigment method for quick, it is characterized in that: on the described colour developing thin slice epiphragma is arranged.
5, as claim 1 or 3 described food synthetic pigment method for quick, it is characterized in that: under described absorption thin slice and the colour developing thin slice base plate paper is arranged, two thin slices are fixed on the base plate paper.
6, a kind of food synthetic pigment detects box, it is characterized in that comprising:
Acid-base modifier: comprising concentration of hydrochloric acid is that 0.5-3.0mol/L acid regulator and naoh concentration are the 0.5-3.0mol/L alkaline conditioner;
The pigment test card;
The synthetic dyestuff developer: synthetic dyestuff developer volume ratio is volume ratio=1 of ammoniacal liquor and ethanol: 5-15;
PH test paper.
7, food synthetic pigment as claimed in claim 6 detects the using method of box:
(1) after solid sample shreds, take by weighing 10 and restrain in 50 ml beakers, add 30 milliliters of pure water and soak after 10 minutes, the sample thief soak solution is in another beaker, and is to be measured; Fluid sample is directly got 30 milliliters in beaker, and is to be measured;
(2) with the pH value of pH test paper test sample liquid or sample soak solution,, can directly detect if between 3-5; If greater than 5, or, then drip acid-base modifier to pH 3-5 less than 3;
(3) get a slice pigment test card and will post absorption one of thin slice and insert in the sample liquid, swing gently and allowed its abundant adsorpting pigment in two minutes;
(4) test card that will be adsorbed with pigment is with pure water flush away impurity and unnecessary pigment;
(5) drip two synthetic dyestuff developers to the absorption thin slice, throw off the transparent epiphragma on the colour developing thin slice, with the test card doubling, allow absorption thin slice and colour developing thin slice be in contact with one another overlapping, open test card, observing the colour developing thin slice has non-pigment to adhere to, and natural colouring matter can not adhere to, and the synthetic dyestuff that is that adheres to is arranged.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100299959A CN101644681B (en) | 2008-08-05 | 2008-08-05 | Method for quickly detecting food synthetic pigment and detection box of food synthetic pigment |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008100299959A CN101644681B (en) | 2008-08-05 | 2008-08-05 | Method for quickly detecting food synthetic pigment and detection box of food synthetic pigment |
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| CN101644681A true CN101644681A (en) | 2010-02-10 |
| CN101644681B CN101644681B (en) | 2011-08-17 |
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| CN104316583A (en) * | 2014-11-17 | 2015-01-28 | 广东省潮州市质量计量监督检测所 | Electrochemical sensor for rapidly measuring carmine and citrine and preparing method thereof |
| CN104820044A (en) * | 2015-05-05 | 2015-08-05 | 梧州市产品质量检验所 | Assay method of synthetic colorant in foods |
| CN105115965A (en) * | 2015-07-27 | 2015-12-02 | 南昌大学 | Soft drink synthetic pigment fast detection method and kit |
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| CN104316583A (en) * | 2014-11-17 | 2015-01-28 | 广东省潮州市质量计量监督检测所 | Electrochemical sensor for rapidly measuring carmine and citrine and preparing method thereof |
| CN104820044A (en) * | 2015-05-05 | 2015-08-05 | 梧州市产品质量检验所 | Assay method of synthetic colorant in foods |
| CN105115965A (en) * | 2015-07-27 | 2015-12-02 | 南昌大学 | Soft drink synthetic pigment fast detection method and kit |
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| CN105203538B (en) * | 2015-10-10 | 2017-10-20 | 福州大学 | Method and detection pipe based on gold nano-material Visual retrieval freshness of meat |
| CN105651770A (en) * | 2016-01-08 | 2016-06-08 | 昆明泊银科技有限公司 | Double-folding type formaldehyde test paper and formaldehyde detection method |
| CN105588834A (en) * | 2016-01-08 | 2016-05-18 | 江南大学 | Method for intelligently displaying carbon dioxide of respiratory climacteric type fresh-cut fruits and vegetables |
| CN105588834B (en) * | 2016-01-08 | 2018-01-23 | 江南大学 | A kind of method of the carbon dioxide intelligent display of climacteric type fresh-cut fruit and vegetable |
| CN108344733A (en) * | 2017-01-25 | 2018-07-31 | 吴智良 | Occult blood test paper |
| WO2018137527A1 (en) * | 2017-01-25 | 2018-08-02 | 吴智良 | Fecal occult blood test pad |
| CN108344733B (en) * | 2017-01-25 | 2020-05-01 | 吴智良 | Occult blood test paper |
| CN109115753A (en) * | 2017-06-22 | 2019-01-01 | 吴智良 | Occult blood test device and method |
| CN107703129A (en) * | 2017-09-08 | 2018-02-16 | 瓮福达州化工有限责任公司 | A kind of detection method of colouring agent aberration |
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Owner name: GUANGDONG OASIS BIOCHEM FOOD SAFETY SCIENCE AND TE Free format text: FORMER NAME: GUANGZHOU OASIS BIOCHEMISTRY TECHNOLOGY CO., LTD. |
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Inventor after: Pan Hua Inventor after: Xie Junping Inventor after: Xu Meiming Inventor before: Pan Hua Inventor before: Xie Junping |
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Free format text: CORRECT: INVENTOR; FROM: PAN HUA XIE JUNPING TO: PAN HUA XIE JUNPING XU MEIMING |
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Address after: 902 room 7, No. 510663, 1st floor, science Avenue, Guangzhou hi tech Industrial Development Zone, Guangdong, China Patentee after: GUANGDONG DAYUAN OASIS FOOD SAFETY TECHNOLOGY CO., LTD. Address before: 902 room 7, No. 510663, 1st floor, science Avenue, Guangzhou hi tech Industrial Development Zone, Guangdong, China Patentee before: Guangzhou Oasis Biochemistry Technology Co.,Ltd. |
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Addressee: Zhao Sha Document name: Notification of Passing Examination on Formalities |
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Effective date of registration: 20180914 Address after: 510663 Guangdong Guangzhou high tech Industrial Development Zone, Science City, open source road 11, A2 third. Co-patentee after: Guangdong Zhong Da Da Detection Technology Co., Ltd. Patentee after: GUANGDONG DAYUAN OASIS FOOD SAFETY TECHNOLOGY CO., LTD. Address before: 510663 room 902, 7 science and Technology Street, science and technology Avenue, Guangzhou high tech Industrial Development Zone, Guangdong Patentee before: GUANGDONG DAYUAN OASIS FOOD SAFETY TECHNOLOGY CO., LTD. |