CN105203538A - Method and detecting tube for visually detecting meat freshness based on gold nanoparticle material - Google Patents
Method and detecting tube for visually detecting meat freshness based on gold nanoparticle material Download PDFInfo
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Abstract
The invention discloses a method for visually detecting meat freshness based on a gold nanoparticle material. According to the method, different biogenic amines released by the meat in different freshness can hydrolyze a reducing agent precursor and generate different reducible matters, so that the visible detection for the meat freshness can be realized by inducing the chromogenic reaction between the reducible matters and the gold nanoparticle material under the condition of the existence of silver ion. Besides, the invention also provides a detecting tube for visually detecting the meat freshness. The detecting tube comprises a transparent tube body and a transparent tube cover connected with the transparent tube body; a gel is arranged in the transparent tube cover; the gold nanoparticle material, the reducing agent precursor and the silver nitrate are contained in the gel. The detecting tube can be used for performing the conventional quick test on the meat freshness or real-time indicating the freshness of the meat within the shelf life. The method and the detecting tube provided by the invention can be used for realizing the quick, accurate and real-time detection for the meat freshness; the detection cost is low; the operation is simple and convenient; the detecting tube can be conveniently carried and stored.
Description
Technical field
The invention belongs to meat products detection technique field, be specifically related to a kind of method based on gold nano-material Visual retrieval freshness of meat and detector tube thereof.
Background technology
The corruption of meat normally due to butchering, process and pollute by external microbe in the process such as circulation and enzyme effect caused by.Not only there is serious change at organoleptic properties, color, elasticity, smell etc. in corrupt meat, and destroys the nutritional labeling of meat, and simultaneously because microbial life metabolism can produce noxious material, the meat after edible corruption can cause food poisoning.Consider, for meat production department and consumer provide a kind of instrument tool of food spoilage degree that indicates to be of great significance from guarantee health and economic environmental angle.
Detect what announced at present or indicate in the related invention patent of freshness of meat, a kind of detection method (CN104568933A) of cold fresh meat freshness, a kind of method and device (CN101936912A) detecting fish freshness based on olfaction visualization, a kind of manufacture method (CN102050273A) of wrappage of energy sensing food freshness, a kind of food freshness indicator (CN1662813A) is all produce total volatile basic nitrogen (TVB-N) to the change of indicator color by cold fresh meat, judge the freshness of cold fresh meat, reach the object predicting cold fresh meat shelf life, Estimation of The Fish Freshness Test paper (CN104297235A) is also the principle utilizing pH variable color, by detecting the change of fish epidermal mucus pH, then comparing with standard color comparison card, judging Estimation of The Fish Freshness, a kind of to indicate CO
2for the food freshness indicating card of characteristic gas and method for making (CN104048958A) absorb CO by indicating card
2variable color judges food freshness.Above patent of invention is all based on traditional pH Cleaning Principle, and realized the object of prediction food freshness by traditional indicator discoloration such as Bromothymol blue, methyl red, dibromophenolphthalein, bromcresol purple, but the change of its colourity is narrower, stability of material is poor.Some patents of invention are also had to provide special determining instrument, the method (CN102297930A) with predicting freshness of meat is identified as a kind of, a kind of near-infrared spectrum technique detects the method (CN103424374) of hairtail freshness fast, fast a kind of, the method (CN104458888A) of sensitive detection freshness of meat, fresh-water fishes freshness ultrasound measuring instrument and detection method (CN103983693A), a kind of fillet Noninvasive Measuring Method of Freshness (CN103439285A) based on high light spectrum image-forming, but these methods all need to adopt special instrument, realized detecting by the operative skill of special personnel and specialty, be unfavorable for applying widely and promoting.
Summary of the invention
The object of the present invention is to provide a kind of method based on gold nano-material Visual retrieval freshness of meat and detector tube thereof, it can realize quick, accurate, the real-time detection of freshness of meat, and its testing cost is low, easy and simple to handle, and is easy to carry and preserves.
For achieving the above object, the present invention adopts following technical scheme:
A kind of method based on gold nano-material Visual retrieval freshness of meat, it is that the different biogenic amine energy reductive hydrolysis agent presomas utilizing the meat of different freshness to discharge generate different reducing substanceses, thus under silver ion existent condition, reducing substances and gold nano-material is made to carry out chromogenic reaction, generate the golden contracted payment particle of different wave length or different-thickness and produce color change, realizing the Visual retrieval of freshness of meat.
Described meat comprises beef, the flesh of fish, chicken, duck and seafood products;
Described reductive agent presoma is resorcinol acetate (No. CAS: 102-29-4; Molecular structural formula:
), acetic acid-4-hydroxylphenyl ester (No. CAS: 3233-32-7; Molecular structural formula:
), No. acetylorcinol(CAS: 889140-51-6; Molecular structural formula:
) or hydroquinone diaeetate (No. CAS: 1205-91-0; Molecular structural formula:
);
Described gold nano-material is gold nanorods or gold nano grain.
The preparation method of described gold nanorods comprises the steps:
1) synthesis of crystal seed: gold chloride and sodium borohydride solution are reacted 30s under cetyl trimethyl ammonium bromide existence condition, then precipitation 1h at 30 DEG C, obtains crystal seed;
2) synthesis of gold nanorods: under cetyl trimethyl ammonium bromide existence condition, after gold chloride, silver nitrate, hydrochloric acid, ascorbic acid mixing, add the crystal seed prepared by step 1), lucifuge leaves standstill overnight rear centrifugal, gained precipitation is dispersed in cetyl trimethyl ammonium bromide solution again, obtains gold nanorods solution;
In step 1), the mol ratio of gold chloride and sodium borohydride solution is 1:1-10;
Step 2) in gold chloride, liquor argenti nitratis ophthalmicus, hydrochloric acid and ascorbic acid mol ratio be 5:1:200:8; The volume range of every 2mmol hydrochloric acid crystal seed used is 0.5-5.0mL.
The preparation method of described gold nano grain is: add gold chloride in water after, boiling is heated under magnetic agitation condition, add citric acid three sodium solution rapidly, when solution colour becomes peony, continue heating 15-20min, then stop heating, naturally cool to room temperature, obtain described gold nano grain solution;
The mol ratio of described trisodium citrate and gold chloride is 2-5:1.
A kind of detector tube based on gold nano-material Visual retrieval freshness of meat comprises transparent tube body and the transparent pipe lid be connected on body, body and pipe lid form a closed cavity, gel is provided with, containing gold nano-material, reductive agent presoma and silver nitrate in described gel in described pipe lid.
The preparation method of described detector tube comprises the steps:
1) by after gold nano-material ultrasonic disperse, jointly join in gel solution with reductive agent presoma, silver nitrate, mixing;
2) gel solution getting mixing moves on in pipe lid, and under room temperature, after cooling forming, encapsulation stores, and obtains described detector tube.
Described gel is Ago-Gel, aquagel or polyvinyl alcohol gel;
Step 2) in reductive agent presoma, silver nitrate, gel solution mol ratio be 10-120:1:32; The amount of every 32 μm of ol gel solution gold nano-materials used is 20-300 μ L.
Described detector tube can be used for meat products freshness conventional quick test or for indicating the freshness of meat products in shelf life in real time.
The concrete grammar that described routine detects fast is: close upper tube cap add meat leachate in the body of detector tube after, or encapsulate together with meat after detector tube is uncapped, place 1-5h under room temperature or refrigerated condition after, by the change of gel color in detector tube to judge the freshness of meat.
The concrete grammar of described real-time instruction is: in meat products production run, jointly encapsulate with meat after detector tube is uncapped, dispatch from the factory, transport, store, in this process by the change of gel color in detector tube to reflect the freshness of meat in shelf life in real time.
remarkable advantage of the present invention is:the present invention adopts novel nano-material as developer, gel film color can be made to change abundanter, measurement result is more accurate, expensive instrument is not needed to detect, the freshness of meat can be judged by naked eyes, can realize the detection that freshness of meat is quick, accurate, real-time, and its testing cost is low, easy and simple to handle, and is easy to carry and preserves.
Embodiment
A kind of method based on gold nano-material Visual retrieval freshness of meat, it is that the biogenic amine utilizing the meat of different freshness to discharge is different, different reducing substanceses can be generated by reductive hydrolysis agent presoma, thus under silver ion existence condition, reducing substances and gold nano-material is made to carry out chromogenic reaction, generate the golden contracted payment particle of different wave length or different-thickness and produce color change, realizing the Visual retrieval of freshness of meat.
Described meat comprises beef, the flesh of fish, chicken, duck and seafood products;
Described reductive agent presoma is resorcinol acetate (No. CAS: 102-29-4; Molecular structural formula:
), acetic acid-4-hydroxylphenyl ester (No. CAS: 3233-32-7; Molecular structural formula:
), No. acetylorcinol(CAS: 889140-51-6; Molecular structural formula:
) or hydroquinone diaeetate (No. CAS: 1205-91-0; Molecular structural formula:
);
Described gold nano-material is gold nanorods or gold nano grain.
The preparation method of described gold nanorods comprises the steps:
1) synthesis of crystal seed: gold chloride and sodium borohydride solution are reacted 30s under cetyl trimethyl ammonium bromide existence condition, then precipitation 1h at 30 DEG C, obtains crystal seed;
2) synthesis of gold nanorods: under cetyl trimethyl ammonium bromide existence condition, after gold chloride, silver nitrate, hydrochloric acid, ascorbic acid mixing, add the crystal seed prepared by step 1), lucifuge leaves standstill overnight rear centrifugal, gained precipitation is dispersed in cetyl trimethyl ammonium bromide solution again, obtains gold nanorods solution.
In step 1), the mol ratio of gold chloride and sodium borohydride solution is 1:1-10;
Step 2) in gold chloride, liquor argenti nitratis ophthalmicus, hydrochloric acid and ascorbic acid mol ratio be 5:1:200:8; The volume range of every 2mmol hydrochloric acid crystal seed used is 0.5-5.0mL.
The preparation method of described gold nano grain is: add gold chloride in water after, boiling is heated under magnetic agitation condition, add citric acid three sodium solution rapidly, when solution colour becomes peony, continue heating 15-20min, then stop heating, naturally cool to room temperature, obtain gold nano grain solution;
The mol ratio of described trisodium citrate and gold chloride is 2-5:1.
A kind of detector tube based on gold nano-material Visual retrieval freshness of meat comprises transparent tube body and the transparent pipe lid be connected on body, body and pipe lid form a closed cavity, gel is provided with, containing gold nano-material, reductive agent presoma and silver nitrate in described gel in described pipe lid.
The preparation method of described detector tube comprises the steps:
1) by after gold nano-material ultrasonic disperse, jointly join in gel solution with reductive agent presoma, silver nitrate, mixing;
2) gel solution getting mixing moves on in pipe lid, and under room temperature, after cooling forming, encapsulation stores, and obtains described detector tube.
Described gel is Ago-Gel, aquagel or polyvinyl alcohol gel;
Step 2) in reductive agent presoma, silver nitrate, gel solution mol ratio be 10-120:1:32; The amount of every 32 μm of ol gel solution gold nano-materials used is 20-300 μ L.
Described detector tube can be used for meat products freshness conventional quick test or for indicating the freshness of meat products in shelf life in real time.
The concrete grammar that described routine detects fast is: close upper tube cap add meat leachate in the body of detector tube after, or encapsulate together with meat after detector tube is uncapped, place 1-5h under room temperature or refrigerated condition after, by the gel color in detector tube and colorimetric card being contrasted, thus judge the freshness of meat.
The concrete grammar of described real-time instruction is: in meat products production run, jointly encapsulate with meat after detector tube is uncapped, dispatch from the factory, transport, store, by the gel color in detector tube and colorimetric card being contrasted in this process, thus reflect the freshness of meat in shelf life in real time.
The preparation method of described colorimetric card is: sealed up for safekeeping together with fresh beef, the flesh of fish, chicken, duck, seafood etc. respectively by the detector tube made, store under room temperature or 4 DEG C of refrigerated conditions, take pictures afterwards at set intervals, draw the time dependent colorimetric card of gel color; Adopt the content of TVB-N under semimicro nitriding mensuration different time in GB/T5009.44-2003 " analytical approach of meat quail hygienic standard " simultaneously, to determine freshness of meat under the corresponding time, thus obtain the colorimetric card that different gel Color pair answers freshness of meat.
More being convenient to make content of the present invention understand, below in conjunction with embodiment, technical solutions according to the invention are described further, but the present invention being not limited only to this.
embodiment one
The synthesis of gold nanorods:
1) synthesis of crystal seed: in 9.75mL cetyl trimethyl ammonium bromide (0.10mol/L), add 0.25mL gold chloride (9.71mmol/L), then add 0.3mL sodium borohydride solution (0.01mol/L), after stirring 30s, precipitation 1h at 30 DEG C, obtains umbrinaceous crystal seed;
2) synthesis of gold nanorods: in 100mL cetyl trimethyl ammonium bromide (0.10mol/L), add 5.15mL gold chloride (9.7mmol/L), 0.1mL liquor argenti nitratis ophthalmicus (0.10mol/L), 2mL hydrochloric acid solution (1.00mol/L), 0.8mL ascorbic acid (0.10mol/L), 0.5mL crystal seed is added after mixing, lucifuge leaves standstill rear centrifugal (12000rpm overnight, 20min), gained precipitation is dispersed in 150.00mL cetyl trimethyl ammonium bromide solution (33.00mmol/L) again, obtains gold nanorods solution.
embodiment two
The synthesis of gold nanorods:
1) synthesis of crystal seed: in 9.75mL cetyl trimethyl ammonium bromide (0.10mol/L), add 0.25mL gold chloride (9.71mmol/L), then add 1.2mL sodium borohydride solution (0.02mol/L), after stirring 30s, precipitation 1h at 30 DEG C, obtains umbrinaceous crystal seed;
2) synthesis of gold nanorods: in 100mL cetyl trimethyl ammonium bromide (0.10mol/L), add 5.15mL gold chloride (9.71mmol/L), 0.1mL liquor argenti nitratis ophthalmicus (0.10mol/L), 2mL hydrochloric acid solution (1.00mol/L), 0.8mL ascorbic acid (0.10mol/L), 0.5mL crystal seed is added after mixing, lucifuge leaves standstill rear centrifugal (12000rpm overnight, 20min), gained precipitation is dispersed in 150.00mL cetyl trimethyl ammonium bromide solution (33.00mmol/L) again, obtains gold nanorods solution.
embodiment three
The synthesis of gold nano grain:
1mL gold chloride (24.4mmol/L) is joined in the conical flask that 100mL water is housed, be heated to boiling under magnetic stirring, add rapidly the citric acid three sodium solution (20.00mmol/L) of 2.5mL, now can there is Rapid Variable Design in the color of solution, finally present peony to colourless again to purple by faint yellow, after then continuing heating 15min, stop heating, naturally cool to room temperature, obtain gold nano grain solution.
embodiment four
The synthesis of gold nano grain:
1mL gold chloride (24.4mmol/L) is joined in the conical flask that 100mL water is housed, be heated to boiling under magnetic stirring, add rapidly the citric acid three sodium solution (48.00mmol/L) of 2.5mL, now can there is Rapid Variable Design in the color of solution, finally present peony to colourless again to purple by faint yellow, after then continuing heating 15min, stop heating, naturally cool to room temperature, obtain gold nano grain solution.
embodiment five
Gold nanorods is to the response of various common biogenic amine:
Gold nanorods solution 20 μ L prepared by Example one, add 3 μ L liquor argenti nitratis ophthalmicuses (0.05mol/L), then 25 μ L resorcinol acetate solution (0.06mol/L) mixings are added, finally add the different 1.80mmol/L alkamines of 300 μ L (blank sample, tyrasamine, histamine, spermidine, spermine, ethylenediamine, tryptamines, β-phenyl ethylamine, cadaverine, trimethylamine), place after 2h, solution colour is respectively: pink, yellow, light yellow, turquoise, large red, light green-gray in color, light yellow, yellow green, celadon, light yellow.
embodiment six
Gold nanorods is to the response of various common biogenic amine:
Gold nanorods solution 100 μ L prepared by Example two, add 3 μ L liquor argenti nitratis ophthalmicuses (0.05mol/L), then 25 μ Lacetylorcinol solution (0.40mol/L) mixings are added, finally add the different 1.80mmol/L alkamines of 300 μ L (blank sample, tyrasamine, histamine, spermidine, spermine, ethylenediamine, tryptamines, β-phenyl ethylamine, cadaverine, trimethylamine), after placing 4h, solution colour is respectively: pink, yellow green, yellow, green grey, large red, kermesinus, yellow, green, light green-gray in color, yellow green.
embodiment seven
Gold nano grain is to the response of various common biogenic amine:
Gold nano grain solution 200 μ L prepared by Example three, add 3 μ L liquor argenti nitratis ophthalmicuses (0.05mol/L), then 25 μ L acetic acid-4-hydroxylphenyl ester solution (0.40mol/L) mixings are added, finally add the different 1.80mmol/L alkamines of 300 μ L (blank sample, tyrasamine, histamine, spermidine, spermine, ethylenediamine, tryptamines, β-phenyl ethylamine, cadaverine, trimethylamine), place after 1h, solution colour be respectively pink, orange red, orange-yellow, yellow, buff, yellow, orange-yellow, yellow, yellow, orange red.
embodiment eight
Gold nano grain is to the response of various common biogenic amine:
Gold nano grain solution 300 μ L prepared by Example four, add 3 μ L liquor argenti nitratis ophthalmicuses (0.05mol/L), then 25 μ L hydroquinone diaeetate solution (0.72mol/L) mixings are added, finally add the different 1.80mmol/L alkamines of 300 μ L (blank sample, tyrasamine, histamine, spermidine, spermine, ethylenediamine, tryptamines, β-phenyl ethylamine, cadaverine, trimethylamine), after placing 3h, solution colour is respectively pink, yellow, yellow, buff, buff, buff, yellow, buff, buff, yellow.
embodiment nine
Preparation based on the detector tube of gold nanorods:
1) gold nanorods pre-service: gold nanorods solution embodiment one prepared is ultrasonic rear for subsequent use;
2) assembling of detector tube: get 200 μ L gained gold nanorods solution, join in the Ago-Gel (15.86mmol/L) of 2mL water-bath temperature control, add 5 μ L resorcinol acetates solution (2.00mol/L) after mixing, then add 20 μ L liquor argenti nitratis ophthalmicus (0.05mol/L) mixings;
3) detector tube is shaping: get 100 μ L mixed liquors and move on in pipe lid, ambient temperatare is put after 5min makes its cooling forming and encapsulated and get final product, and then it is stored at 4 DEG C.
embodiment ten
Preparation based on the detector tube of gold nano grain:
1) gold nano grain pre-service: gold nano grain solution embodiment two prepared is ultrasonic rear for subsequent use;
2) assembling of detector tube: get 200 μ L gained gold nano grain solution, join in the chitosan gel rubber (15.86mmol/L) of 2mL water-bath temperature control, add 30 μ Lacetylorcinol solution (2.00mol/L) after mixing, then add 20 μ L liquor argenti nitratis ophthalmicus (0.05mol/L) mixings;
3) detector tube is shaping: get 100 μ L mixed liquors and move on in pipe lid, ambient temperatare is put after 5min makes its cooling forming and encapsulated and get final product, and then it is stored at 4 DEG C.
embodiment 11
Preparation based on the detector tube of gold nanorods:
1) gold nanorods pre-service: gold nanorods solution embodiment one prepared is ultrasonic rear for subsequent use;
2) assembling of detector tube: get 200 μ L gained gold nanorods solution, join (15.86mmol/L) in the polyvinyl alcohol gel of 2mL water-bath temperature control, add 60 μ L hydroquinone diaeetates solution (2.00mol/L) after mixing, then add 20 μ L liquor argenti nitratis ophthalmicus (0.05mol/L) mixings;
3) detector tube is shaping: get 100 μ L mixed liquors and move on in pipe lid, ambient temperatare is put after 5min makes its cooling forming and encapsulated and get final product, and then it is stored at 4 DEG C.
embodiment 12
Preparation based on the detector tube of gold nanorods:
1) gold nanorods pre-service: gold nanorods solution embodiment two prepared is ultrasonic rear for subsequent use;
2) assembling of detector tube: get 200 μ L gained gold nanorods solution, join in the polyvinyl alcohol gel (15.86mmol/L) of 2mL water-bath temperature control, add 30 μ L acetic acid-4-hydroxylphenyl ester solution (2.00mol/L) after mixing, then add 20 μ L liquor argenti nitratis ophthalmicus (0.05mol/L) mixings;
3) detector tube is shaping: get 100 μ L mixed liquors and move on in pipe lid, ambient temperatare is put after 5min makes its cooling forming and encapsulated and get final product, and then it is stored at 4 DEG C.
embodiment 13
Detector tube 2 prepared by Example nine, after uncapping respectively with etc. heavy 2 parts of beef one reinstate preservative film and encapsulate, then 2 parts of beef are placed respectively under room temperature and 4 DEG C of refrigerated conditions, in placement 1,2,4,7d time record detector tube in gel color, and the freshness of beef is judged according to the colorimetric card of preparation, its result is respectively in table 1 and table 2.
Table 1 beef room temperature deposits the freshness result after different time
The freshness result after different time is deposited in the refrigeration of table 2 beef
embodiment 14
Detector tube 2 prepared by Example ten, after uncapping respectively with etc. heavy 2 parts oppress one and reinstate preservative film and encapsulate, then 2 portions of flesh of fish are placed respectively under room temperature and 4 DEG C of refrigerated conditions, in placement 1,2,4,7d time record detector tube in gel color, and the freshness of the flesh of fish is judged according to the colorimetric card of preparation, its result is respectively in table 3 and table 4.
Table 3 oppress room temperature deposit different time after freshness result
Table 4 is oppressed and is refrigerated the freshness result after depositing different time
embodiment 15
Detector tube 8 prepared by Example 11, reinstate preservative film with 8 portions of chicken one of the equivalent depositing different number of days respectively after uncapping to encapsulate, then chicken is taken out detector tube after placing 2h under room temperature and 4 DEG C of refrigerated conditions, gel color in record detector tube, and the freshness of chicken is judged according to the colorimetric card of preparation, its result is respectively in table 5 and table 6.
Table 5 chicken room temperature deposits the freshness result after different time
The freshness result after different time is deposited in the refrigeration of table 6 chicken
embodiment 16
Detector tube 8 prepared by Example 12, reinstate preservative film with 8 portions of ducks one of the equivalent depositing different number of days respectively after uncapping to encapsulate, then duck is taken out detector tube after placing 3h under room temperature and 4 DEG C of refrigerated conditions, gel color in record detector tube, and the freshness of duck is judged according to the colorimetric card of preparation, its result is respectively in table 7 and table 8.
Table 7 duck room temperature deposits the freshness result after different time
The freshness result after different time is deposited in the refrigeration of table 8 duck
Method provided by the invention and detector tube can realize quick, accurate, the real-time detection of freshness of meat, and its testing cost is low, easy and simple to handle, and are easy to carry and preserve.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (10)
1. the method based on gold nano-material Visual retrieval freshness of meat, it is characterized in that: the different biogenic amine energy reductive hydrolysis agent presomas utilizing the meat of different freshness to discharge generate different reducing substanceses, thus under silver ion existent condition, make reducing substances and gold nano-material carry out chromogenic reaction, realize the Visual retrieval of freshness of meat.
2. according to claim 1 based on the method for gold nano-material Visual retrieval freshness of meat, it is characterized in that: described meat comprises beef, the flesh of fish, chicken, duck and seafood products;
Described reductive agent presoma is
,
,
or
;
Described gold nano-material is gold nanorods or gold nano grain.
3. according to claim 2 based on the method for gold nano-material Visual retrieval freshness of meat, it is characterized in that: the preparation method of described gold nanorods comprises the steps:
1) synthesis of crystal seed: gold chloride and sodium borohydride solution are reacted 30s under cetyl trimethyl ammonium bromide existence condition, then precipitation 1h at 30 DEG C, obtains crystal seed;
2) synthesis of gold nanorods: under cetyl trimethyl ammonium bromide existence condition, after gold chloride, silver nitrate, hydrochloric acid, ascorbic acid mixing, add the crystal seed prepared by step 1), lucifuge leaves standstill overnight rear centrifugal, gained precipitation is dispersed in cetyl trimethyl ammonium bromide solution again, obtains gold nanorods solution;
In step 1), the mol ratio of gold chloride used and sodium borohydride is 1:1-10;
Step 2) in the mol ratio of gold chloride, silver nitrate, hydrochloric acid and ascorbic acid be 5:1:200:8; The amount that every 2mmol hydrochloric acid adds crystal seed is 0.5-5.0mL.
4. according to claim 2 based on the method for gold nano-material Visual retrieval freshness of meat, it is characterized in that: the preparation method of described gold nano grain is: add gold chloride in water after, boiling is heated under magnetic agitation condition, add citric acid three sodium solution rapidly, when solution colour becomes peony, continue heating 15-20min, then stop heating, naturally cool to room temperature, obtain gold nano grain solution;
The mol ratio of trisodium citrate used and gold chloride is 2-5:1.
5. one kind as claimed in claim 1 based on the detector tube of gold nano-material Visual retrieval freshness of meat, it is characterized in that: described detector tube comprises transparent tube body and the transparent pipe lid be connected on body, body and pipe lid form a closed cavity, gel is provided with, containing gold nano-material, reductive agent presoma and silver nitrate in described gel in described pipe lid.
6., according to claim 5 based on the detector tube of gold nano-material Visual retrieval freshness of meat, it is characterized in that: its preparation method comprises the steps:
1) by after gold nano-material ultrasonic disperse, jointly join in gel solution with reductive agent presoma, silver nitrate, mixing;
2) gel solution getting mixing moves on in pipe lid, and under room temperature, after cooling forming, encapsulation stores, and obtains described detector tube.
7. according to claim 6 based on the detector tube of gold nano-material Visual retrieval freshness of meat, it is characterized in that: described gel is Ago-Gel, aquagel or polyvinyl alcohol gel;
Step 2) in reductive agent presoma, silver nitrate, gel solution mol ratio be 10-120:1:32; The amount of every 32 μm of ol gel solution gold nano-materials used is 20-300 μ L.
8. an application for detector tube as claimed in claim 5, is characterized in that: for the conventional quick test of meat products freshness or for indicating the freshness of meat products in shelf life in real time.
9. the application of detector tube according to claim 8, it is characterized in that: the concrete grammar that described routine detects fast is: add meat sample or its leachate in the body of detector tube after, close upper tube cap, or encapsulate together with meat after detector tube is uncapped, place 1-5h under room temperature or refrigerated condition after, by the change of gel color in detector tube to judge the freshness of meat.
10. the application of detector tube according to claim 8, it is characterized in that: the concrete grammar of described real-time instruction is: in meat products production run, jointly encapsulate with meat after detector tube is uncapped, dispatch from the factory, transport, store, in this process by the change of gel color in detector tube to reflect the freshness of meat products in shelf life in real time.
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