CN101793838A - Rapid detection method for hydrogen peroxide in fresh milk - Google Patents
Rapid detection method for hydrogen peroxide in fresh milk Download PDFInfo
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- CN101793838A CN101793838A CN201010137661A CN201010137661A CN101793838A CN 101793838 A CN101793838 A CN 101793838A CN 201010137661 A CN201010137661 A CN 201010137661A CN 201010137661 A CN201010137661 A CN 201010137661A CN 101793838 A CN101793838 A CN 101793838A
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Abstract
The invention discloses a rapid detection method for hydrogen peroxide in fresh milk, which belongs to the field of rapid detection of food. The method can be successfully applied to the rapid detection of adulteration hydrogen peroxide in milk. Through improved optimized reagent preparation, the response sensitivity and color development effect of the method are improved. The method has the advantages of cheap reagents used, simple operation steps, good color development effect, high sensitivity and lower detection limit of 5 mu g per milliliter of milk. The storage stability of the reagents can at least reach half a year without affecting detection sensitivity, and the reagents are suitable for on-site qualitative semi-quantitative detection.
Description
Technical field
Content of the present invention belongs to food fast detecting field, relates to the method for quick of hydrogen peroxide in a kind of fresh milk.
Background technology
Dairy products market quality security situation allows of no optimist at present, the behavior of mingling fraud grows in intensity, especially the melamine incident has brought huge harm for vast consumer, after the Sanlu incident, insider and consumers in general very pay close attention to dairy food quality, and the key issue of dairy food quality is a milk source purity.In fact, fresh milk is mingled problem and is of long duration, Qinglingshan Mouuntains board milk powder before more than ten years, just occurred and mixed the case that nitrite is poisoned the baby with poison because of raw material milk, there is the media report lawless person that protein hydrolysate powder (with acid hydrolysis products therefroms such as old leather shoes, belt, suitcases) is added in the water mixing milk before more than 2 years again, to improve total protein content.By dairy food quality is is in recent years researched and analysed as can be known, melamine is only mingled a kind of in the composition for dairy products, remove this also have such as protein hydrolysate powder, nitrite, formaldehyde, sodium carbonate, ammonium salt, urea etc. nearly tens kinds adulterant mixed fresh milk by the lawless person, wherein many compositions to the harm of human body more greater than melamine! According to relevant person in the know, have now and more generally the strong oxidizer hydrogen peroxide is added in the raw material milk as antiseptic.
Hydrogen peroxide claims hydrogen peroxide again, is added into to play effects such as bleaching, anticorrosion and deodorizing in the food.Milk is the profuse material of a kind of nutritional labeling, and microorganism is breeding very easily in a large number therein.Microbiological indicator reaches acquisition criteria in the raw material milk in order to make. and some people that make profit as one's only aim will be to wherein adding some antiseptics to suppress the breeding of bacterium, and a kind of as antiseptic of hydrogen peroxide, colourless, tasteless, be easy to get, do not influence the smell of cow's milk after a small amount of the adding, but it add the quality that affiliation directly influences finished milk, influence eater's health.Therefore, need seek a kind of method fast and accurately and detect whether mix hydrogen peroxide in the fresh milk.
Detecting the method for mingling hydrogen peroxide in the fresh milk at present mainly is test paper method, guaiac resin test solution method and traditional chemical development process.Wherein test paper method is easy to operate, but it is desirable not to the utmost to detect limit value; Guaiac resin test solution method detectability is low, but the reagent price is more expensive, reagent preparation and detection complex steps, and the reagent shelf-life is very short; It is relatively low to detect limit value in the traditional chemical development process, but reagent can not guarantee the quality for a long time, should promptly join promptly and use.
Summary of the invention
The object of the present invention is to provide a kind of improving the method for quick of hydrogen peroxide in the fresh milk that the chemical reagent development process is a principle, thereby reach the purpose of qualitative and half-quantitative analysis.
For achieving the above object, the technical solution used in the present invention is: get one of clean color comparison tube to wherein adding milk sample to be checked, at first adding mass concentration in the milk sample is the KI of 5-25%, after the vibration, what add mass concentration again and be 0.5-5% contains acid-starch solution, shake up, left standstill several minutes, compare with colorimetric card then; Sample: KI wherein suckles: the volume that contains acid-starch solution is 5: 1: 1; If blueness or purple occurring in various degree all can be judged to positive findings and show promptly in the milk sample and be mixed with hydrogen peroxide that the adulterated milk sample then be white.
Contain the NaCl of 10-18g, the glacial acetic acid of 10-16mL of the present invention containing in every 100mL solution in the acid-starch solution.
The present invention adopts and improves chemical staining method (is principle with acid catalysis starch-KI colour developing), hydrogen peroxide in the fast detecting fresh milk.This method is simple to operate, and the reagent term of validity is long, and is highly sensitive fast, is limited to 5ppm under detecting.
The chromogenic reaction principle:
H
2O
2+KI→I
2；
I
2+ starch → blue compound;
CH
3COOH+KI→HI；
HI+H
2O
2→I
2；
I
2+ starch → blue compound.
Wherein acetic acid has catalytic chemistry colour developing effect; The starch solution antisepsis improves reagent shelf-life purpose to reach.
Compare with existing technology, the present invention has following characteristics:
1, simple starch solution usually can be just putrid and deteriorated after several days, and the starch solution of using among the present invention, through improving preparation, but not only catalysis chromogenic reaction improves sensitivity greatly, be limited to 5ppm under detecting, and make reagent have long preservation and effect, change without short-term;
2, the present invention uses reagent cheap, simple to operate, is applicable to that on-site qualitative semi detects.
Description of drawings
Fig. 1 is that hydrogen peroxide detects color developing effect figure in the fresh milk of the present invention.
Embodiment
Embodiment 1: get one of clean color comparison tube to the milk sample to be checked that wherein adds 5ml, at first in the milk sample, add the 1ml mass concentration and be 5% KI, after the vibration, add the 1ml mass concentration again and be 0.5% contain acid-starch solution, contain the NaCl of 18g, the glacial acetic acid of 16mL said containing in every 100mL solution in the acid-starch solution.Shake up, left standstill several minutes, if blueness occurs in various degree or purple all can be judged to positive findings (promptly show in the milk sample and be mixed with hydrogen peroxide), adulterated milk sample (blank) be not white.Sxemiquantitative is judged and can be compared by colorimetric card.
Embodiment 2: get one of clean color comparison tube to the milk sample to be checked that wherein adds 5ml, at first in the milk sample, add the 1ml mass concentration and be 15% KI, after the vibration, add the 1ml mass concentration again and be 1% contain acid-starch solution, contain the NaCl of 15g, the glacial acetic acid of 10mL said containing in every 100mL solution in the acid-starch solution.Shake up, left standstill several minutes, if blueness occurs in various degree or purple all can be judged to positive findings (promptly show in the milk sample and be mixed with hydrogen peroxide), adulterated milk sample (blank) be not white.Sxemiquantitative is judged and can be compared by colorimetric card.
Embodiment 3: get one of clean color comparison tube to the milk sample to be checked that wherein adds 5ml, at first in the milk sample, add the 1ml mass concentration and be 25% KI, after the vibration, add the 1ml mass concentration again and be 2% contain acid-starch solution, contain the NaCl of 12g, the glacial acetic acid of 15mL said containing in every 100mL solution in the acid-starch solution.Shake up, left standstill several minutes, if blueness occurs in various degree or purple all can be judged to positive findings (promptly show in the milk sample and be mixed with hydrogen peroxide), adulterated milk sample (blank) be not white.Sxemiquantitative is judged and can be compared by colorimetric card.
Embodiment 4: get one of clean color comparison tube to the milk sample to be checked that wherein adds 5ml, at first in the milk sample, add the 1ml mass concentration and be 10% KI, after the vibration, add the 1ml mass concentration again and be 4% contain acid-starch solution, contain the NaCl of 10g, the glacial acetic acid of 13mL said containing in every 100mL solution in the acid-starch solution.Shake up, left standstill several minutes, if blueness occurs in various degree or purple all can be judged to positive findings (promptly show in the milk sample and be mixed with hydrogen peroxide), adulterated milk sample (blank) be not white.Sxemiquantitative is judged and can be compared by colorimetric card.
Embodiment 5: get one of clean color comparison tube to the milk sample to be checked that wherein adds 5ml, at first in the milk sample, add the 1ml mass concentration and be 20% KI, after the vibration, add the 1ml mass concentration again and be 5% contain acid-starch solution, contain the NaCl of 13g, the glacial acetic acid of 11mL said containing in every 100mL solution in the acid-starch solution.Shake up, left standstill several minutes, if blueness occurs in various degree or purple all can be judged to positive findings (promptly show in the milk sample and be mixed with hydrogen peroxide), adulterated milk sample (blank) be not white.Sxemiquantitative is judged and can be compared by colorimetric card.
With reference to Fig. 1, hydrogen peroxide method for quick color developing effect in the fresh milk
| The color comparison tube numbering | ??0 | ??1 | ??2 | ??3 | ??4 | ??5 |
| Contain H 2O 2Concentration (%) | ??0 | ??0.01 | ??0.005 | ??0.001 | ??0.0005 | ??0.0001 |
| H 2O 2Content (μ g/mL breast) | ??0 | ??100 | ??50 | ??10 | ??5 | ??1 |
| Sample presents color | Milky | Mazarine | Bluish violet | Lavender | Orange | Milky |
Claims (2)
1. the method for quick of hydrogen peroxide in the fresh milk, it is characterized in that: get one of clean color comparison tube to wherein adding milk sample to be checked, at first adding mass concentration in the milk sample is the KI of 5-25%, after the vibration, what add mass concentration again and be 0.5-5% contains acid-starch solution, shake up, left standstill several minutes, compare with colorimetric card then; Sample: KI wherein suckles: the volume that contains acid-starch solution is 5: 1: 1; If blueness or purple occurring in various degree all can be judged to positive findings and show promptly in the milk sample and be mixed with hydrogen peroxide that the adulterated milk sample then be white.
2. hydrogen peroxide method for quick in the fresh milk according to claim 1 is characterized in that: contain the NaCl of 10-18g, the glacial acetic acid of 10-16mL said containing in every 100mL solution in the acid-starch solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010137661A CN101793838A (en) | 2010-04-01 | 2010-04-01 | Rapid detection method for hydrogen peroxide in fresh milk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010137661A CN101793838A (en) | 2010-04-01 | 2010-04-01 | Rapid detection method for hydrogen peroxide in fresh milk |
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| Publication Number | Publication Date |
|---|---|
| CN101793838A true CN101793838A (en) | 2010-08-04 |
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ID=42586646
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010137661A Pending CN101793838A (en) | 2010-04-01 | 2010-04-01 | Rapid detection method for hydrogen peroxide in fresh milk |
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|---|---|
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104215633A (en) * | 2014-09-11 | 2014-12-17 | 中国水稻研究所 | Paper chip detection method for rapidly determining content of amylose in paddy rice and application system adopted in paper chip detection system for rapidly determining content of amylose in paddy rice |
| CN106872453A (en) * | 2017-01-11 | 2017-06-20 | 浙江大学 | Starch potassium iodide microchannel plastic sheeting detection strip and preparation method and purposes |
| CN107328767A (en) * | 2017-05-25 | 2017-11-07 | 新希望乳业股份有限公司 | The quick determination method of peroxidase in a kind of guaranteed milk |
| CN107831161A (en) * | 2017-12-06 | 2018-03-23 | 广东轻工职业技术学院 | Small molecule containing acetate is used as the new application of catalyst as class peroxidase |
-
2010
- 2010-04-01 CN CN201010137661A patent/CN101793838A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104215633A (en) * | 2014-09-11 | 2014-12-17 | 中国水稻研究所 | Paper chip detection method for rapidly determining content of amylose in paddy rice and application system adopted in paper chip detection system for rapidly determining content of amylose in paddy rice |
| CN106872453A (en) * | 2017-01-11 | 2017-06-20 | 浙江大学 | Starch potassium iodide microchannel plastic sheeting detection strip and preparation method and purposes |
| CN106872453B (en) * | 2017-01-11 | 2019-08-30 | 浙江大学 | Starch potassium iodide microchannel plastic film detects strip and preparation method and purposes |
| CN107328767A (en) * | 2017-05-25 | 2017-11-07 | 新希望乳业股份有限公司 | The quick determination method of peroxidase in a kind of guaranteed milk |
| CN107831161A (en) * | 2017-12-06 | 2018-03-23 | 广东轻工职业技术学院 | Small molecule containing acetate is used as the new application of catalyst as class peroxidase |
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Application publication date: 20100804 |