CN104215633A - Paper chip detection method for rapidly determining content of amylose in paddy rice and application system adopted in paper chip detection system for rapidly determining content of amylose in paddy rice - Google Patents
Paper chip detection method for rapidly determining content of amylose in paddy rice and application system adopted in paper chip detection system for rapidly determining content of amylose in paddy rice Download PDFInfo
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- CN104215633A CN104215633A CN201410459782.5A CN201410459782A CN104215633A CN 104215633 A CN104215633 A CN 104215633A CN 201410459782 A CN201410459782 A CN 201410459782A CN 104215633 A CN104215633 A CN 104215633A
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Abstract
The invention discloses a paper chip detection system for rapidly determining the content of amylose in paddy rice. The paper chip detection system comprises a potassium iodide paper chip and a developing solution; a production method of the potassium iodide paper chip comprises the steps: arranging a hydrophilic channel network on a paper substrate, wherein the hydrophilic channel network comprises a central area and a channel surrounding the central area, and the part of the paper substrate, except for the hydrophilic channel network, is hydrophobic; dropwise adding a KI-Na2CO3 aqueous solution in the central area, and naturally permeating the KI-Na2CO3 aqueous solution in the hydrophilic channel network; and naturally drying the paper substrate to obtain the potassium iodide paper chip. The developing solution is an acid-strong oxidant aqueous solution. The invention also provides a detection method adopting the paper chip detection system. The detection method comprises the following steps of preparing a to-be-detected paddy rice sample solution, a paddy rice amylose standard sample solution, a blank sample solution and the like. By adopting the paper chip detection method and the paper chip detection system, the content of the amylose in the paddy rice can be rapidly and conveniently determined.
Description
Technical field
The present invention relates to food inspection field, particularly refill chip detection method and the detection system used of amylose content in a kind of fast detecting rice.
Background technology
Starch is a kind of natural polysaccharide, is the principal ingredient of cereal, has been widely used at present various food and non-food stuff industry.The character of starch is most important for its final use.Direct-connected content of starch in starch and gelatinization, gelation, viscosity, solubleness, swelling capacity, digestibility and the resistance of starch are closely related.Therefore amylose content is an important indicator evaluating cereal organoleptic quality and processing quality.In addition, amylopectin and amylopectin ratios are to affect the key factor that resistant starch forms.And amylose content is the important indicator in rice quality conventional sense in rice.Therefore measuring amylose content in rice has very important significance and realistic price.
The detection method of having reported at present multiple amylose content in document, wherein tradition and classical detection method are iodine colorimetry the most.Its principle is that iodine and amylose clathration generate blue inclusion compound, and the final blue depth showing is relevant to the content of amylose, can measure the wherein content of amylose by colourimetry.But due to the amylopectin in starch also can with the purpuric inclusion compound of elemental iodine clathration, produce certain impact to detecting.Therefore, many documents have adopted dual wavelength colourimetry, three-wavelength colourimetry and six wavelength colorimetric determinations to measure to reach more accurately.Generally, colourimetry process is relatively loaded down with trivial details.And spectrophotometric colorimetric and data handling procedure have been saved in birth and the application of full-automatic Continuous Flow Analysis instrument, greatly simplify analytic process.In addition, many new detection methods in document, are also reported, as new detection methods such as iodine affinity titrimetry, efficient size exclusion chromatography (SEC), near infrared spectroscopic method, enzyme process (concanavalin method), differential scanning calorimetry, thermogravimetries.But these analytical approachs all need specific expensive instrument and equipment, need in specific laboratory, carry out, be difficult for promoting in common lab.
Summary of the invention
The technical problem to be solved in the present invention is to provide refill chip detection method and the system used that in a kind of rice, amylose content rapid and convenient is measured.
In order to solve the problems of the technologies described above, the invention provides the refill sheet detection system of amylose content Fast Measurement in a kind of rice:
Comprise potassium iodide refill sheet (, regioselectivity absorption has the refill sheet of potassium iodide) and nitrite ion;
The method for making of described potassium iodide refill sheet is:
1., hydrophilic channel network is set in paper substrates, described hydrophilic channel network comprises center and the passage around center, the quantity of described passage is at least 4, the inner of each passage is connected with center, the outer end of each passage arranges detection zone;
Described paper substrates is hydrophobicity except the part beyond hydrophilic channel network;
Remarks explanation: described paper substrates is test paper (conventional test paper);
2., drip KI-Na in center
2cO
3aqueous solution, described KI-Na
2cO
3aqueous solution is naturally osmotic (under the effect of capillary force) in hydrophilic channel network; Naturally, after drying, obtain potassium iodide refill sheet;
Described KI-Na
2cO
3in aqueous solution, KI and Na
2cO
3concentration identical, be 30mmolL
-1;
Described nitrite ion is acid-strong oxidizer aqueous solution, and described strong oxidizer is the oxygenant of oxidisability higher than elemental iodine; In described acid-strong oxidizer aqueous solution, the concentration of acid is 50～400mmolL
-1, the concentration of strong oxidizer is 30～120mmolL
-1.
Improvement as the refill sheet detection system of amylose content Fast Measurement in rice of the present invention:
Nitrite ion is the H containing HAC
2o
2aqueous solution, the described H containing HAC
2o
2in aqueous solution, the concentration of HAC is 200mmolL
-1, H
2o
2concentration be 60mmolL
-1.
Further improvement as the refill sheet detection system of amylose content Fast Measurement in rice of the present invention: described hydrophilic channel network is a rice font hydrophilic channel network;
Described rice font hydrophilic channel network comprises center and equably around 8 passages of center;
Drip the KI-Na of 9～11 μ L (being preferably 10 μ L) in described center
2cO
3aqueous solution.
Remarks explanation: be a meter font hydrophilic channel network due to what arrange, therefore, a chip has 8 detection zones (seeing Fig. 2), one of them detection zone is for the detection of blank sample, four detection zones are used for the detection of standard model, remain the detection of three detection zones for testing sample, can measure three unknown samples simultaneously or same sample is carried out to three replicate determinations.
Remarks explanation: the structure of close and distant aquaporin network can design according to practical application.
Further improvement as the refill sheet detection system of amylose content Fast Measurement in rice of the present invention:
Thereby adopt following either method to realize and in paper substrates, prepare hydrophilic channel network:
Hexadecyl trichlorosilane chemical modification in conjunction with vacuum plasma treatment technology, wax seal technology, hexadecyl trichlorosilane chemical modification in conjunction with ultraviolet degradation technology, hexadecyl trichlorosilane chemical modification in conjunction with corona treatment techniques.
Remarks explanation: said method all should known technology.
The present invention also provides the detection method of utilizing above-mentioned refill sheet detection system to carry out simultaneously, comprises the steps:
1), prepare Rice Samples solution to be measured, rice amylose standard sample solution and blank sample solution; The setting of described rice amylose standard sample solution is at least the standard sample solution of two or more different amylose content;
Remarks explanation: rice amylose standard sample is the Rice Samples that amylose content is known.
2) draw, respectively 0.4～0.6 μ L (being preferably 0.5 μ L) Rice Samples solution to be measured, rice amylose standard sample solution and blank sample solution and be added drop-wise in the different detection zones of potassium iodide refill sheet, leave standstill and volatilize; Thereby corresponding Rice Samples to be measured detection zone, rice amylose standard sample detection zone and the blank detection zone of obtaining respectively;
3), drip 9～11 μ L (being preferably 10 μ L) nitrite ion in the center of potassium iodide refill sheet; Colour developing 10～30min (being preferably 20min); When the colour developing of blank detection zone and only for showing when yellow, enter following step 4), otherwise return to step 1) re-start detection;
Remarks explanation: when observing the colour developing of blank detection zone and being only, while showing yellow, to show to detect in normal condition;
4), utilize following either method to judge:
Method A,
By the color intensity difference of naked-eye observation and comparison rice amylose standard sample detection zone and Rice Samples to be measured detection zone, tentatively judge the content range of amylose in Rice Samples to be measured;
Method B,
Adopt instrument to obtain after the chip picture after colour developing, measure respectively the color intensity that obtains Rice Samples to be measured detection zone, rice amylose standard sample detection zone and blank detection zone by software; By the color intensity of rice amylose standard sample detection zone gained color intensity deduction blank sample detection zone, obtain the color intensity of standard model; By the color intensity of Rice Samples to be measured detection zone gained color intensity deduction blank sample detection zone, obtain the color intensity of Rice Samples to be measured (by test agent);
According to the corresponding relation drawing standard curve between amylose content and the color intensity of the standard sample solution of different amylose contents, by in the above-mentioned typical curve of color intensity substitution of Rice Samples to be measured (by test agent), thereby finally obtain the amylose content of Rice Samples to be measured (by test agent).
Improvement as detection method of the present invention: in described method B, adopt scanner scanning, or camera, mobile phone photograph, thereby obtain the chip picture after colour developing; Software is Photoshop or ImageJ software.
Further improvement as detection method of the present invention: rice amylose standard sample solution arranges the standard sample solution of following 4 kinds of different amylose contents: 1.5%, 10.4%, 16.2%, 26.4%; Above-mentioned % is % by weight.
Further improvement as detection method of the present invention:
Prepare Rice Samples solution to be measured, rice amylose standard sample solution and blank sample solution according to the standard NY/T 55-1987 of the Ministry of Agriculture, in preparation process, sample weighting amount is doubled.
, be specially:
Take Rice Samples/rice amylose standard sample 100mg ± 0.2mg to be measured, adding 0.5mL volumetric concentration is 95% ethanol, thereby makes the moistening dispersion of sample; Add again 4.5mL 1mol/L NaOH solution, be placed in boiling water bath and boil 10min, be cooled to room temperature after water be settled to 50mL; Thereby corresponding Rice Samples solution to be measured, the rice amylose standard sample solution of obtaining respectively;
Cancel taking of above-mentioned Rice Samples/rice amylose standard sample to be measured, sample weighting amount is set is 0mg; All the other contents are the same, thereby obtain blank sample solution.
In detection method of the present invention: step 1) in because the disposal route of Rice Samples to be measured (by test agent), standard model is identical, final liquor capacity is identical, so step 4) the typical curve of method B gained be to set up between amylose content by standard model and final colored intensity, then can directly read the amylose content in Rice Samples to be measured (by test agent) by the colored intensity of Rice Samples to be measured (by test agent).
Detection principle of the present invention is: nitrite ion flows to detection zone by hydrophilic channel from center, potassium iodide on oxygenant reduction potassium iodide refill sheet is wherein elemental iodine, the amylose clathration of elemental iodine and detection zone is aobvious blue, can carry out quantitative and semi-quantitative mensuration according to the blue depth.
The present invention is by the color intensity of Photoshop or ImageJ software measurement detection zone, thereby after the color intensity of deduction blank sample detection zone, gets standard samples and by the color intensity of test agent.The color intensity drawing standard curve of according to standard sample, and according to quantitatively being calculated by the amylose content in test agent by the color intensity of test agent.
The present invention has following technical advantage:
1. potassium iodide refill sheet is stable, can normal temperature, preserve for a long time.That is, the elemental iodine that carries out clathration with amylose is to generate by the instant oxidation of iodide ion, and not volatile, chip is easily preserved.
2. this method can realize quantitative and semi-quantitative detection, and naked eyes can be realized half-quantitative detection.
Remarks explanation:
Sxemiquantitative: corresponding to method A.Can judge which two standard model content the amylose content in Rice Samples to be measured (by test agent) is between between by naked eyes according to final colored intensity.As use is in the present invention that amylose content is 1.5%, 10.4%, 16.2%, 26.4% standard model, according to colored intensity, can judge by amylose content in test agent it is to be positioned at following which value range: 1.5-10.4%, 10.4-16.2%, 16.2-26.4% or be greater than 26.4%;
Quantitative: corresponding to method B, read the value of the colored intensity of detection zone by software, after the color intensity of deduction blank sample detection zone, get standard samples and by the color intensity of test agent, then Criterion curve, calculates by an exact value of amylose content in test agent.
3. mensuration process does not need specific installation, and cost is low, can detect whenever and wherever possible.
4, the present invention releases a kind of new detection means and platform, adopts refill sheet detection means cheap and easy to get to replace the detecting instrument of the various complex and expensive of reporting in current document, amylose is detected and be easy to realize in common lab.
5, elemental iodine is volatile, if therefore simply directly adsorb elemental iodine on refill sheet, in elemental iodine a few minutes of absorption, completely, the refill sheet of preparation is difficult for preservation in volatilization.And we adopt absorption KI in the present invention, and then generate in real time elemental iodine by the method for adding oxygenant in the time detecting, therefore increase the storage characteristics of chip.
6, existing iodine colorimetry need adopt special detecting instrument, as spectrophotometer or full-automatic Continuous Flow Analysis instrument.And the software and hardware that the refill chip detection method that the present invention proposes needs is all common, as scanner, camera, mobile phone Photoshop and ImageJ, make whole testing process be very easy to realize and popularize, and cost is also very low.
In sum, preparation that the present invention is intended to utilize refill sheet to have is simple, portable, reagent/sample consumption is low, can multivariate detection, simple to operate, without advantages such as the instrument and equipments of complex and expensive, a kind of simple, quick, cheap, portative refill chip detection method that can carry out amylose content in on-site measurement rice is provided.Amylose content in the method sxemiquantitative fast or quantitative mensuration rice.
Brief description of the drawings
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Fig. 1 is the schematic flow sheet that the preparation of potassium iodide refill sheet and amylose are measured:
In figure:
(a): the refill sheet with the close and distant aquaporin network of meter font that adopts hexadecyl trichlorosilane chemical modification to prepare in conjunction with vacuum plasma treatment technology;
(b): the center at (a) drips KI-Na
2cO
3solution, solution spreads to detection zone along passage under capillary force effect;
(c): the potassium iodide refill sheet (b) obtaining after volatilizing;
(d): the detection zone at (c) drips respectively sample solution;
(e): on basis (d), drip nitrite ion in the center of refill sheet, nitrite ion spreads to detection zone under capillary force effect;
(f): the refill sheet after colour developing;
(g): the refill picture after the colour developing that scanning obtains.
Fig. 2 is the schematic diagram of the potassium iodide refill sheet in Fig. 1;
In figure, white portion is hydrophilic passage area (, hydrophilic channel network structure), and gray area is hydrophobic region; Numeral 1-8 represents 8 detection zones.
Fig. 3 is the affect figure of HAc concentration on color sample intensity in nitrite ion;
Amylose content in the sample wherein adopting is 26.4%.
Fig. 4 is KI-Na
2cO
3the affect figure of KI concentration on color sample intensity in aqueous solution;
Amylose content in the sample wherein adopting is 26.4%.
Embodiment
Embodiment 1, prepare the refill sheet detection system of amylose content Fast Measurement in rice:
This refill sheet detection system comprises potassium iodide refill sheet (, regioselectivity absorption has the refill sheet of potassium iodide) and nitrite ion;
1, the method for making of potassium iodide paper chip base is:
1., taking test paper (conventional test paper) as paper substrates, adopt usual manner (for example for hexadecyl trichlorosilane chemical modification in conjunction with vacuum plasma treatment technology, wax seal technology, hexadecyl trichlorosilane chemical modification in conjunction with ultraviolet degradation technology, hexadecyl trichlorosilane chemical modification in conjunction with corona treatment techniques etc.) that hydrophilic channel network---rice font hydrophilic channel network is set on test paper.
Described rice font hydrophilic channel network comprises center and equably around 8 passages of center; The inner of each passage is connected with center, the outer end of each passage arranges detection zone.
Remarks explanation: center is rounded, and diameter is about 5mm.The length of each passage is about 4mm, and width is about 2mm; Each detection zone is the circle that diameter is about 3.5mm.
Test paper is hydrophobicity except the part beyond rice font hydrophilic channel network.
2., drip the KI-Na of 10 μ L in center
2cO
3aqueous solution; Under the effect of capillary force, KI-Na
2cO
3aqueous solution slowly infiltrates into all hydrophilic channel regions.Treat KI-Na
2cO
3aqueous solution can obtain potassium iodide refill sheet after permeating completely and volatilizing.KI-Na
2cO
3in aqueous solution, KI and Na
2cO
3concentration be 30mmolL respectively
-1.
Remarks explanation: be a meter font hydrophilic channel network due to what arrange, that is, have 8 passages around center equably; Therefore, a chip has 8 detection zones (seeing Fig. 2), one of them detection zone is for the detection of blank sample, four detection zones are used for the detection of standard model, remain the detection of three detection zones for testing sample, can measure three unknown samples simultaneously or same sample is carried out to three replicate determinations.
2, nitrite ion is selected the H of HAc
2o
2aqueous solution, wherein the concentration of HAc is 200mmolL
-1, H
2o
2concentration be 60mmolL
-1.
The refill chip detection method of amylose content Fast Measurement in the rice that the detection system of embodiment 2, employing embodiment 1 gained is carried out:
1), prepare Rice Samples solution to be measured, rice amylose standard sample solution and blank sample solution according to the standard NY/T 55-1987 of the Ministry of Agriculture, in preparation process, the sample size taking is doubled.
Same Rice Samples to be measured carries out three replicate determinations or measures three unknown samples simultaneously; The rice amylose standard sample solution of 4 kinds of different known amylose contents is set: the standard sample of these 4 kinds of known amylose contents: 1.5%, 10.4%, 16.2%, 26.4%.
, be specially:
Take Rice Samples/rice amylose standard sample 100mg ± 0.2mg to be measured, adding 0.5mL volumetric concentration is 95% ethanol, thereby makes the moistening dispersion of sample; Add again 4.5mL 1mol/L NaOH solution, be placed in boiling water bath and boil 10min, be cooled to room temperature after water be settled to 50mL; Thereby corresponding Rice Samples solution to be measured, the rice amylose standard sample solution of obtaining respectively;
Cancel taking of above-mentioned Rice Samples/rice amylose standard sample to be measured, sample weighting amount is set is 0mg; All the other contents are the same, thereby obtain blank sample solution.
2) Rice Samples solution to be measured, rice amylose standard sample solution and the blank sample solution of drawing, respectively 0.5 μ L are added drop-wise in the different detection zones of potassium iodide refill sheet, leave standstill and volatilize; Thereby corresponding Rice Samples to be measured detection zone, rice amylose standard sample detection zone and the blank detection zone of obtaining respectively;
Be specially: blank sample solution is added drop-wise to 1, four of detection zone rice amylose standard sample solution and is added drop-wise to respectively detection zone 2-5, and Rice Samples solution to be measured is added drop-wise to respectively detection zone 6-8.Leave standstill and volatilize.
3), drip 10 μ L nitrite ions in the center of potassium iodide refill sheet; Under capillary force effect, nitrite ion is penetrated into detection zone gradually from eight passages respectively.After colour developing 20min, obtain the refill sheet after colour developing.When the colour developing of blank detection zone and only for showing when yellow, enter following step 4), otherwise return to step 1) re-start detection;
4), utilize following either method to judge:
Method A,
By the color intensity difference of naked-eye observation and comparison rice amylose standard sample detection zone and Rice Samples to be measured detection zone, tentatively judge the content range of amylose in Rice Samples to be measured;
Method B,
Adopt instrument to obtain after the chip picture after colour developing, measure respectively the color intensity that obtains 3 Rice Samples to be measured detection zones, 4 rice amylose standard sample detection zones and 1 blank detection zone by software;
By the color intensity of gained color intensity deduction blank sample detection zone, 4 rice amylose standard sample detection zones, obtain respectively the color intensity of 4 standard models;
By the color intensity of gained color intensity deduction blank sample detection zone, 3 Rice Samples to be measured detection zones, obtain respectively the color intensity of 3 Rice Samples to be measured;
According to the corresponding relation drawing standard curve between amylose content and the color intensity of the standard sample solution of above-mentioned 4 kinds of different amylose contents, by in the above-mentioned typical curve of color intensity substitution of Rice Samples to be measured, thereby finally obtain the amylose content of Rice Samples to be measured.
Test 1, (three replicate determinations be set) using rice A to be measured as Rice Samples to be measured, detect according to method described in embodiment 2; Step 4) result of gained is:
The testing result of Rice Samples A is shown in Fig. 1 g, blank sample detection zone 1 is yellow, standard model detection zone 2-5 and testing sample detection zone 6-8 are yellow and add blueness, measure respectively the intensity of 8 detection zone colors with Photoshop, get standard samples respectively after the intensity of blank detection zone and the color intensity of testing sample thereby deduct.Draw and obtain typical curve y=180.39x+3858.2 according to the color intensity corresponding with it of the amylose content in four standard models.Remarks explanation: amylose content in x representative sample, its data unit is %; Y represents the color intensity of counter sample.
The color intensity of Rice Samples A of measuring for three times is updated to and obtains three results after typical curve and be respectively 19.1%, 16.2%, 17.2%, and the amylose content of Rice Samples A is 17.5 ± 1.4%.
Test 2, make rice A to be measured into rice B to be measured, all the other are with experiment 1, and the color intensity of Rice Samples B of measuring for three times is brought into and obtains three results after typical curve and be respectively 10.6%, 10.0%, 7.1%, the amylose content of Rice Samples B is 9.2 ± 1.9%.
Test 3, make rice A to be measured into rice C to be measured, all the other are with experiment 1, and the color intensity of Rice Samples C of measuring for three times is brought into and obtains three results after typical curve and be respectively 29.9%, 27.5%, 25.8%, the amylose content of Rice Samples C is 27.7 ± 2.1%.
Confirmatory experiment 1, rice A to be measured, B, C are detected according to current the used amylose automatic analyzer method based on iodine colorimetric, acquired results is as shown in table 1:
Table 1
| ? | The present invention | Automatic analyzer method |
| Sample A | 17.5±1.4％ | 17.7±0.3％ |
| Sample B | 9.2±1.9％ | 9.6±0.3％ |
| Sample C | 26.9±2.4％ | 26.7±0.4％ |
Through the inspection of t method of inspection, three kinds of Rice Samples there was no significant difference in 0.05 level of significance.
Show that this method is applicable to the mensuration of amylose content in rice; Be particularly useful for the initial screening test to the less demanding amylose content kind of accuracy of detection.
In order fully to prove the importance of various process parameters of the present invention, inventor has also carried out following contrast experiment in invention process:
The impact of HAc concentration on color sample intensity in contrast experiment 1, nitrite ion:
Described nitrite ion is the H containing HAC
2o
2aqueous solution, by the concentration of HAC by 200mmolL
-1make respectively 0,25,50,100,400 into, 800mmolL
-1, the amylose content in the sample of employing is 26.4%; The color intensity of corresponding gained as described in Figure 3.According to Fig. 3, we learn: the optium concentration of HAC is 200mmolL
-1.
Contrast experiment 2, KI-Na
2cO
3the impact of KI concentration on color sample intensity in aqueous solution:
KI-Na
2cO
3in aqueous solution, by the concentration of KI by 30mmolL
-1make respectively 0,7.5,15,60 into, 120mmolL
-1, the amylose content in the sample of employing is 26.4%.The color intensity of corresponding gained as described in Figure 4.According to Fig. 4, we learn: the optium concentration of KI is 30mmolL
-1.
Finally, it is also to be noted that, what more than enumerate is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, can also have many distortion.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (8)
1. the refill sheet detection system of amylose content Fast Measurement in rice, is characterized in that:
Comprise potassium iodide refill sheet and nitrite ion;
One, the method for making of described potassium iodide refill sheet is:
1., hydrophilic channel network is set in paper substrates, described hydrophilic channel network comprises center and the passage around center, the quantity of described passage is at least 4, the inner of each passage is connected with center, the outer end of each passage arranges detection zone;
Described paper substrates is hydrophobicity except the part beyond hydrophilic channel network;
2., drip KI-Na in center
2cO
3aqueous solution, described KI-Na
2cO
3aqueous solution is naturally osmotic in hydrophilic channel network; Naturally, after drying, obtain potassium iodide refill sheet;
Described KI-Na
2cO
3in aqueous solution, KI and Na
2cO
3concentration identical, be 30mmolL
-1;
Two, described nitrite ion is acid-strong oxidizer aqueous solution, and described strong oxidizer is the oxygenant of oxidisability higher than elemental iodine; In described acid-strong oxidizer aqueous solution, the concentration of acid is 50～400mmolL
-1, the concentration of strong oxidizer is 30～120mmolL
-1.
2. the refill sheet detection system of amylose content Fast Measurement in rice according to claim 1, is characterized in that:
Described nitrite ion is the H containing HAC
2o
2aqueous solution, the described H containing HAC
2o
2in aqueous solution, the concentration of HAC is 200mmolL
-1, H
2o
2concentration be 60mmolL
-1.
3. the refill sheet detection system of amylose content Fast Measurement in rice according to claim 1 and 2, is characterized in that:
Described hydrophilic channel network is rice font hydrophilic channel network;
Described rice font hydrophilic channel network comprises center and equably around 8 passages of center;
Drip the KI-Na of 9～11 μ L in described center
2cO
3aqueous solution.
4. the refill sheet detection system of amylose content Fast Measurement in rice according to claim 3, is characterized in that:
Thereby adopt following either method to realize and in paper substrates, prepare hydrophilic channel network:
Hexadecyl trichlorosilane chemical modification in conjunction with vacuum plasma treatment technology, wax seal technology, hexadecyl trichlorosilane chemical modification in conjunction with ultraviolet degradation technology, hexadecyl trichlorosilane chemical modification in conjunction with corona treatment techniques.
5. the detection method of utilizing the refill sheet detection system as described in as arbitrary in claim 1～4 to carry out, is characterized in that comprising the steps:
1), prepare Rice Samples solution to be measured, rice amylose standard sample solution and blank sample solution; The setting of described rice amylose standard sample solution is at least the standard sample solution of two or more different amylose content;
2) draw, respectively 0.4～0.6 μ L Rice Samples solution to be measured, rice amylose standard sample solution and blank sample solution and be added drop-wise in the different detection zone of potassium iodide refill sheet, leave standstill and volatilize; Thereby corresponding Rice Samples to be measured detection zone, rice amylose standard sample detection zone and the blank detection zone of obtaining respectively;
3), drip 9～11 μ L nitrite ions in the center of potassium iodide refill sheet; Colour developing 10～30min; When the colour developing of blank detection zone and only for showing when yellow, enter following step 4), otherwise return to step 1) re-start detection;
4), utilize following either method to judge:
Method A,
By the color intensity difference of naked-eye observation and comparison rice amylose standard sample detection zone and Rice Samples to be measured detection zone, tentatively judge the content range of amylose in Rice Samples to be measured;
Method B,
Adopt instrument to obtain after the chip picture after colour developing, measure respectively the color intensity that obtains Rice Samples to be measured detection zone, rice amylose standard sample detection zone and blank detection zone by software; By the color intensity of rice amylose standard sample detection zone gained color intensity deduction blank sample detection zone, obtain the color intensity of standard model; By the color intensity of Rice Samples to be measured detection zone gained color intensity deduction blank sample detection zone, obtain the color intensity of Rice Samples to be measured;
According to the corresponding relation drawing standard curve between amylose content and the color intensity of the standard sample solution of different amylose contents, by in the above-mentioned typical curve of color intensity substitution of Rice Samples to be measured, thereby finally obtain the amylose content of Rice Samples to be measured.
6. the detection method of utilizing refill sheet detection system to carry out according to claim 5, is characterized in that: in described method B, adopt scanner scanning, or camera, mobile phone photograph, thereby obtain the chip picture after colour developing; Software is Photoshop or ImageJ software.
7. the detection method of utilizing refill sheet detection system to carry out according to claim 6, is characterized in that:
Rice amylose standard sample solution arranges the standard sample solution of following 4 kinds of different amylose contents: 1.5%, 10.4%, 16.2%, 26.4%; Above-mentioned % is % by weight.
8. according to the detection method of utilizing refill sheet detection system to carry out described in claim 5,6 or 7, it is characterized in that:
Prepare Rice Samples solution to be measured, rice amylose standard sample solution and blank sample solution according to the standard NY/T 55-1987 of the Ministry of Agriculture, in preparation process, sample weighting amount is doubled.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105181690A (en) * | 2015-09-28 | 2015-12-23 | 北京福德安科技有限公司 | 12-channel micro-flow rapid colorimetric water quality detection device |
| CN105067784B (en) * | 2015-07-22 | 2016-08-24 | 中国水稻研究所 | Utilize the physicochemical character index of rice quality to the method judging rice eating-quality |
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| CN114317678A (en) * | 2021-12-31 | 2022-04-12 | 港龙生物技术（深圳）有限公司 | Biological paper chip, high-flux multi-connection detection microporous plate device, preparation method and kit for multi-connection detection of vaginitis |
| CN114740166A (en) * | 2022-03-15 | 2022-07-12 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | Stable and convenient sweet potato amylose content determination method |
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| CN105067784B (en) * | 2015-07-22 | 2016-08-24 | 中国水稻研究所 | Utilize the physicochemical character index of rice quality to the method judging rice eating-quality |
| CN105181690A (en) * | 2015-09-28 | 2015-12-23 | 北京福德安科技有限公司 | 12-channel micro-flow rapid colorimetric water quality detection device |
| CN106353312A (en) * | 2016-10-09 | 2017-01-25 | 中国农业科学院茶叶研究所 | Quick detection method of tea polyphenol contents on basis of micro-fluidic paper chip technology |
| CN106353312B (en) * | 2016-10-09 | 2019-06-25 | 中国农业科学院茶叶研究所 | A kind of polyphenol content rapid detection method based on micro-fluidic core chip technology |
| CN106807462A (en) * | 2017-01-13 | 2017-06-09 | 湖南师范大学 | A kind of acid etch modifies the preparation method of micro-fluidic paper chip |
| CN106807462B (en) * | 2017-01-13 | 2019-05-10 | 湖南师范大学 | A kind of acid etch modifies the preparation method of micro-fluidic paper chip |
| CN109855928A (en) * | 2018-12-04 | 2019-06-07 | 界首市万花巢生物科技有限公司 | A method of extracting starch and content of starch detection from potato |
| CN114317678A (en) * | 2021-12-31 | 2022-04-12 | 港龙生物技术（深圳）有限公司 | Biological paper chip, high-flux multi-connection detection microporous plate device, preparation method and kit for multi-connection detection of vaginitis |
| CN114317678B (en) * | 2021-12-31 | 2023-11-10 | 港龙生物技术（深圳）有限公司 | Biological paper chip, high-throughput multi-detection microplate device, preparation method and kit for vaginal inflammation multi-detection |
| CN114740166A (en) * | 2022-03-15 | 2022-07-12 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | Stable and convenient sweet potato amylose content determination method |
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