CN103105392A - Production method and application of patterned biochemical analysis test paper - Google Patents
Production method and application of patterned biochemical analysis test paper Download PDFInfo
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- CN103105392A CN103105392A CN2013100191150A CN201310019115A CN103105392A CN 103105392 A CN103105392 A CN 103105392A CN 2013100191150 A CN2013100191150 A CN 2013100191150A CN 201310019115 A CN201310019115 A CN 201310019115A CN 103105392 A CN103105392 A CN 103105392A
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Abstract
The invention discloses a production method and an application of patterned biochemical analysis test paper. The test paper is of a compound structure comprising wax and absorbent paper. The production method comprises the steps of: melting the solid wax into liquid on heating equipment; immersing a metal template with a required pattern into the liquid wax to further heat for 5-10 minutes; printing the metal template with the liquid wax as a stamp on the surface of the absorbent paper; absorbing the liquid wax on the metal template by the absorbent paper; cooling to form a plurality of millimeter-sized hydrophilic channels taking the wax as a hydrophobic barrier; and adding a biochemical analysis detection reagent in a set reaction area to obtain the patterned biochemical analysis test paper. The patterned biochemical analysis test paper is applied to multi-index biochemical analysis of various liquid samples such as urine, blood, serum, saliva and water; and the production method has the advantages of low cost and the like, and is simple and quick to operate.
Description
Technical field
The present invention relates to biochemical analysis product development and application thereof, a kind of patterned biochemical analysis test paper, its method for making and application are provided especially.
Background technology
Test paper has been widely used in actual life as a kind of analysis means with low cost, simple to operate, for example, surveys pregnant test paper, pH test paper, urine detection test paper etc.Compare with traditional method for making that is used for the biochemical analysis test paper that many indexes detects, one-shot forming of the present invention, simple to operate, consuming time less, cost is low, is particularly useful for all kinds of small-size laboratories and field fabrication.This patterned biochemical analysis test paper is also referred to as laboratory (Lab-on-paper) on micro-fluidic chip on paper (Paper-based microfluidics) or paper.
the patterned biochemical analysis test paper method for making of bibliographical information mainly comprises photoetch method at present, the paraffin print process, drafting method etc., their main limitation be related instrument (for example, the photoetching instrument, the wax spray printer, machinery drawing instrument etc.) with material (for example, photoresist SU8, the special-purpose paraffin of printer, dimethyl silicone polymers etc.) cost is very high and uncommon, operation steps is relatively loaded down with trivial details time-consuming simultaneously, and the professional skill that operating personnel are had relatively high expectations, thereby be unsuitable for all kinds of small-size laboratories or field condition fabricating patterned biochemical analysis test paper, limited this novel many indexs biochemical analysis test paper at widespread use (the Patterned Paper as a Platform for Inexpensive of numerous areas, Low-Volume, Portable Bioassays. Andres W. Martinez, Scott T. Phillips, Manish J. Butte and George M. Whitesides. Angew. Chem. Int. Ed. 2007, 46, 1318 – 1320, Fabrication and Characterization of Paper-Based Microfluidics Prepared in Nitrocellulose Membrane By Wax Printing. Yao Lu, Weiwei Shi, Jianhua Qin and Bingcheng Lin. Anal. Chem. 2010,82,329 – 335, Low-Cost Printing of Poly (dimethylsiloxane) Barriers To Define Microchannels in Paper Derek A. Bruzewicz, Meital Reches, and George M. Whitesides. Anal. Chem. 2008,80,3387-3392).
Summary of the invention
The purpose of this invention is to provide a kind of method for making and application of patterned biochemical analysis test paper.
The method for making concrete steps of patterned biochemical analysis test paper are:
First with solid wax at firing equipment (electric hot plate for example, regulate the melt temperature of a little higher than wax used of its temperature) on be melted into liquid, the metal form that will have again required pattern immerses and continues heating 5-10 minute in this liquid wax, subsequently the metal form that has been stained with liquid wax is used as seal and is imprinted on the thieving paper surface, liquid wax on metal form is absorbed by thieving paper, a plurality of millimetre-sized hydrophilic channel of cooling rear formation take wax as the hydrophobicity restraining mass adds biochemical analysis to detect reagent at the conversion zone of setting and just obtains patterned biochemical analysis test paper.
Described metal form is for utilizing conventional laser cutting or line process technology, adopts hollow, Yang Ke or cloudy die sinking formula, processes iron, copper or stainless steel and other metal materials and is made.
Described thieving paper is a kind of in filter paper, chromatographic paper and nitrocellulose filter.
The patterned biochemical analysis test paper that the present invention makes is applied to many quota students fractional analysis of the liquid samples such as urine, blood, serum, saliva and water, namely is made into urine detection test paper, blood testing test paper, serum Test paper, saliva Test paper and water quality monitoring test paper; These test paper can filter liquid samples such as urine, blood, serum, saliva and water, remove impurity, the liquid samples such as urine, blood, serum, saliva and water are directly applied to biochemical analysis detect.
The action principle of patterned biochemical analysis test paper provided by the invention is to form millimetre-sized hydrophilic pathway in thieving paper by wax, utilize subsequently its hydrophobic property to come the confined liquid sample to arrive the conversion zone of appointment according to the route of design in advance, complete to detect and analyze.Because wax is separated each conversion zone, the analysis between each index can the phase mutual interference simultaneously.
The present invention replaces the various with high costs and uncommon materials such as photoresist, the special-purpose paraffin of printer, dimethyl silicone polymer with the with low cost and common wax that is easy to get and form millimetre-sized hydrophilic channel on paper; Biochemical analysis test paper is made in metal form one step printing with patterning, do not need any expensive professional instrument, operation steps is simple, especially the figuratum metal form of tool can Reusability, and almost operating personnel are not had the professional skill requirement, the method for making that might replace existing many index tests test paper is fit to business development, thereby promotes the use of at all kinds of small-size laboratories.
Description of drawings
Fig. 1 is that embodiment of the present invention binary channels patterning test paper is made and the biochemical analysis process flow diagram.
Wherein: 1 is the tagger template seal; 2-wax; 3-filter paper; The circle detection district that 4-has dripped 0.6M potassium iodide aqueous solution and horseradish peroxidase/glucose oxidase mixed solution is used for glucose detection; The 5-center adds sample solution district; The square detection zone that 6-has dripped the ethanolic solution of 0.25M citrate buffer solution (PH=1.8) and 3.3mM Tetrabromophenol Blue is used for bovine serum albumin(BSA) (BSA); 7-glucose detection colour developing result; 8-BSA detects the colour developing result.
Fig. 2 is the figure as a result that the embodiment of the present invention is utilized binary channels patterned biochemical analysis test paper that the printing of cored iron template seal makes (left side) and sign rear (right side) before dripping colored water wettability ink and characterizing.
Wherein: 1-binary channels patterned biochemical analysis test paper; The colored water wettability ink of 2-.
Testing result when Fig. 3 is the bovine serum albumin(BSA) (BSA) of a series of variable concentrations of the embodiment of the present invention and glucose (Glucose) mixed solution.
Wherein: 1-glucose (Glucose); 2-bovine serum albumin(BSA) (BSA).
Embodiment
Embodiment:
One, utilize cored iron template seal printing fabricating patterned biochemical analysis test paper:
As shown in Figure 1, first solid wax is melted into liquid on electric hot plate, then the cored iron template is immersed in this liquid wax continues heating 5 minutes, subsequently the cored iron template of being stained with liquid wax is used as seal and is imprinted on the filter paper surface; Because cored iron template itself also has higher temperature, sticking in its lip-deep wax still keeps liquid condition to be absorbed by filter paper, just can block the fiber of filter paper after to be cooled solidifying, form a plurality of millimetre-sized hydrophilic channels (referring to Fig. 2), add biochemical analysis to detect reagent at the conversion zone of setting at last and just can obtain patterned biochemical analysis test paper.
Two, utilize the patterned biochemical analysis test paper for preparing to detect simultaneously glucose and the bovine serum albumin(BSA) (BSA) of variable concentrations:
Border circular areas in test paper and square region are respectively used to the detection of glucose and BSA.
1, glucose detection: at first to add 0.5 μ L concentration be the potassium iodide aqueous solution of 0.6M to the circle detection district on test paper, and then dripping 0.5 μ L volume ratio in the above is the horseradish peroxidase of 5:1 and the mixed solution of glucose oxidase.
2, bovine serum albumin(BSA) (BSA) detects: at first the square detection zone on test paper adds 0.5 μ L citrate buffer solution (concentration is 0.25M, pH=1.8), then continuing in the above to drip 0.5 μ L solvent is that ethanol, concentration are the Tetrabromophenol Blue solution of 3.3mM.
3, solution in step (1) circle detection district and the square detection zone of step (2) is in air (room temperature) after drying, the mixed solution that adds glucose and BSA at application of sample place, center, it (is that 0mM glucose and 0mMBSA mix that mixed solution mixes by concentration corresponding to up and down in Fig. 3, 15mM glucose and 50mMBSA mix, 30mM glucose and 75mMBSA mix, 60mM glucose and 100mMBSA mix, 120mM glucose and 125mMBSA mix), mixed solution is two conversion zones of automatic stream under capillarity, and react and develop the color with wherein dried reagent, wherein the BSA object will obtain blue product with the bromophenol blue reaction under acid condition, and the glucose target thing will generate yellow color product with the potassium iodide reaction under glucose oxidase and the effect of horseradish peroxidase co-catalysis.The color of two kinds of products presents certain linear relationship with the concentration of correspondence analysis thing separately respectively.In other words, in test paper on conversion zone the color of product darker, reflect that indirectly in sample, the correspondence analysis substrate concentration is higher.Testing result when Fig. 3 is the bovine serum albumin(BSA) (BSA) of a series of variable concentrations and glucose (Glucose) mixed solution.
Claims (2)
1. the method for making of a patterned biochemical analysis test paper, it is characterized in that concrete steps are: first solid wax is melted into liquid on firing equipment, the metal form that will have again required pattern immerses and continues heating 5-10 minute in this liquid wax, subsequently the metal form that has been stained with liquid wax is used as seal and is imprinted on the thieving paper surface, liquid wax on metal form is absorbed by thieving paper, a plurality of millimetre-sized hydrophilic channel of cooling rear formation take wax as the hydrophobicity restraining mass adds biochemical analysis to detect reagent at the conversion zone of setting and just obtains patterned biochemical analysis test paper;
Described metal form is for utilizing conventional laser cutting or line process technology, adopts hollow, Yang Ke or cloudy die sinking formula, processes iron, copper or stainless steel metal material and is made;
Described thieving paper is a kind of in filter paper, chromatographic paper and nitrocellulose filter.
2. the application of patterned biochemical analysis test paper according to claim 1, it is characterized in that patterned biochemical analysis test paper is applied to many quota students fractional analysis of urine, blood, serum, saliva and water liquid sample, namely is made into urine detection test paper, blood testing test paper, serum Test paper, saliva Test paper and water quality monitoring test paper; These test paper can filter urine, blood, serum, saliva and water liquid sample, remove impurity, urine, blood, serum, saliva and water liquid sample are directly applied to biochemical analysis detect.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103777003A (en) * | 2014-01-27 | 2014-05-07 | 西南大学 | Portable electrochemical quantitative immunochromatography filtering paper as well as test paper and application thereof |
| CN104181153A (en) * | 2013-05-28 | 2014-12-03 | 郑兆珉 | Handwriting paper raw medical detection test piece and manufacturing method thereof |
| CN104215633A (en) * | 2014-09-11 | 2014-12-17 | 中国水稻研究所 | Paper chip detection method for rapidly determining content of amylose in paddy rice and application system adopted in paper chip detection system for rapidly determining content of amylose in paddy rice |
| CN104215758A (en) * | 2014-09-20 | 2014-12-17 | 桂林理工大学 | Instrument-free quantitative analysis method for fast detecting test paper, and application of instrument-free quantitative analysis method |
| CN104226388A (en) * | 2014-09-20 | 2014-12-24 | 桂林理工大学 | Laser-assisted disposable test paper making method |
| TWI494565B (en) * | 2013-07-09 | 2015-08-01 | Nat Univ Tsing Hua | Method of manufacturing biomedical molecular detection platform and the detection platform manufactured therefrom |
| CN106093027A (en) * | 2016-07-22 | 2016-11-09 | 苏州汶颢芯片科技有限公司 | Heavy metal ion detection chip |
| CN106268990A (en) * | 2016-07-26 | 2017-01-04 | 林捷琳 | A kind of paper micro-flow control chip preparation method based on dot matrix pressure sintering |
| CN106442514A (en) * | 2016-11-24 | 2017-02-22 | 桂林理工大学 | Simple ultrasensitive colorimetric detection method for bivalent copper ions |
| CN109839375A (en) * | 2019-01-30 | 2019-06-04 | 浙江工业大学 | A kind of paper substrate micro-fluidic chip and detection method for examination of glucose concentration |
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| CN101571538A (en) * | 2008-04-29 | 2009-11-04 | 中国科学院大连化学物理研究所 | Patterned biochemical analysis test paper and manufacturing method and application thereof |
| CN101726591A (en) * | 2009-12-09 | 2010-06-09 | 中国科学院大连化学物理研究所 | Wax-patterned nitrocellulose membrane and preparation method and application thereof |
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2013
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| CN101571538A (en) * | 2008-04-29 | 2009-11-04 | 中国科学院大连化学物理研究所 | Patterned biochemical analysis test paper and manufacturing method and application thereof |
| CN101726591A (en) * | 2009-12-09 | 2010-06-09 | 中国科学院大连化学物理研究所 | Wax-patterned nitrocellulose membrane and preparation method and application thereof |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104181153B (en) * | 2013-05-28 | 2017-01-04 | 郑兆珉 | Handwriting paper raw medical detection test piece and manufacturing method thereof |
| CN104181153A (en) * | 2013-05-28 | 2014-12-03 | 郑兆珉 | Handwriting paper raw medical detection test piece and manufacturing method thereof |
| TWI494565B (en) * | 2013-07-09 | 2015-08-01 | Nat Univ Tsing Hua | Method of manufacturing biomedical molecular detection platform and the detection platform manufactured therefrom |
| CN103777003A (en) * | 2014-01-27 | 2014-05-07 | 西南大学 | Portable electrochemical quantitative immunochromatography filtering paper as well as test paper and application thereof |
| CN104215633A (en) * | 2014-09-11 | 2014-12-17 | 中国水稻研究所 | Paper chip detection method for rapidly determining content of amylose in paddy rice and application system adopted in paper chip detection system for rapidly determining content of amylose in paddy rice |
| CN104215758A (en) * | 2014-09-20 | 2014-12-17 | 桂林理工大学 | Instrument-free quantitative analysis method for fast detecting test paper, and application of instrument-free quantitative analysis method |
| CN104226388A (en) * | 2014-09-20 | 2014-12-24 | 桂林理工大学 | Laser-assisted disposable test paper making method |
| CN106093027A (en) * | 2016-07-22 | 2016-11-09 | 苏州汶颢芯片科技有限公司 | Heavy metal ion detection chip |
| CN106268990A (en) * | 2016-07-26 | 2017-01-04 | 林捷琳 | A kind of paper micro-flow control chip preparation method based on dot matrix pressure sintering |
| CN106268990B (en) * | 2016-07-26 | 2018-07-24 | 林捷琳 | A kind of paper micro-flow control chip preparation method based on dot matrix pressure sintering |
| CN106442514A (en) * | 2016-11-24 | 2017-02-22 | 桂林理工大学 | Simple ultrasensitive colorimetric detection method for bivalent copper ions |
| CN106442514B (en) * | 2016-11-24 | 2019-08-13 | 桂林理工大学 | A kind of simple overdelicate bivalent cupric ion colorimetric detection method |
| CN109839375A (en) * | 2019-01-30 | 2019-06-04 | 浙江工业大学 | A kind of paper substrate micro-fluidic chip and detection method for examination of glucose concentration |
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Application publication date: 20130515 |