CN107754961B

CN107754961B - Preparation method of paper microporous plate based on laser printing

Info

Publication number: CN107754961B
Application number: CN201711056027.2A
Authority: CN
Inventors: 朱晓霞; 刘姗
Original assignee: Nantong University
Current assignee: Nantong University
Priority date: 2017-11-01
Filing date: 2017-11-01
Publication date: 2021-01-26
Anticipated expiration: 2037-11-01
Also published as: CN107754961A

Abstract

The invention discloses a method for preparing a paper microporous plate by laser printing, which comprises the following steps: 1) manufacturing a template: designing corresponding templates according to the aperture and the hole spacing of a commercial 96-hole micro-porous plate by using mapping software; 2) printing: using hydrophilic filter paper as a raw material, and printing by using a laser printer, wherein a printed black area is a hydrophobic area, and a white area is a hydrophilic area; the selenium drum used by the laser printer is an original selenium drum. Compared with the traditional paper micropore preparation method, the preparation method does not need an expensive printer, and only needs a conventional office laser printer; no reagent is required to be prepared; no other harsh external conditions are needed, the operation is simple and convenient, and mass production can be realized.

Description

Preparation method of paper microporous plate based on laser printing

Technical Field

The invention relates to a preparation method of a paper-based microporous plate, in particular to a preparation method of a paper-based microporous plate by adopting a laser printing method.

Background

Paper Microfluidic technology (μ PADs) was first proposed by Whitesids team of Harvard university in 2007, and it was prepared by replacing traditional materials with Paper such as filter Paper, chromatography Paper, nitrocellulose membrane, etc. The paper has the advantages of low price, easy obtaining, good biocompatibility, capillary action, small sample consumption, low cost, convenient use and carrying and the like, and is the most common substrate material for the micro-fluidic at present.

In recent years, with the rapid development of Paper-based microfluidic technology, Paper-based materials have attracted great interest to researchers, and have been introduced into and replaced Paper-based Enzyme-linked Immunosorbent assays (p-ELISA) established by conventional 96-well microplates (CM Cheng, AW Martinez, J Gong, CR Mace, ST Phillips, E Carrilho, KA Mirica, GM whitetides, Paper-based ELISA, Angewandte Chemie, 2010, 49(28): 4771-4774.). And capturing a signal picture by using a scanner or a mobile phone, and analyzing the picture by using software. The method not only greatly reduces the cost and time of ELISA detection, but also provides a new channel for bedside detection (AW Martinez, ST Phillips, GM whitetides, E Carrilho. Diagnostics for the detection world: microfluidic paper-based Analytical Chemistry, 2010, 82(1): 3-10.).

Similar to the paper-based microfluidic chip manufacturing technology, the preparation of the paper-based ELISA microporous plate mainly utilizes certain substances to form hydrophobic isolation zones on the surface of filter paper, so that the reaction is only carried out in hydrophilic micropores between the isolation zones. The current manufacturing method mainly comprises the following steps: the photolithography Method comprises immersing a filter paper in an Octadecyl Trichlorosilane (OTS) solution, and then irradiating the filter paper under ultraviolet light to make the exposed area highly hydrophilic and the unexposed area highly hydrophobic, thereby forming a hydrophobic channel (Q He, C Ma, X Hu, H Chemistry, Method for preparing a hydrophobic channel by alkyl silane self-assembly and UV/O3-patterning, Analytical Chemistry, 2013, 85(3): 1327-; wax printing, i.e. printing a pattern of a hydrophobic area by a wax-jet printer, and then baking the paper until wax penetrates into the paper, thereby obtaining a hydrophobic channel (Y Lu, W Shi, L Jiang, J Qin, B Lin, Rapid prototyping of paper-based microfluidics with wax for low-cost, 2010, 30(9):1497 + 1500.); ink jet printing, i.e., removal of the polystyrene coating from the paper by ink jet etching, to yield hydrophilic channels (K Abe, K Kotera, K Suzuki, D characteristics. Inkjet-printed paper fluidic imaging-chemical sensing device, Analytical & biological Chemistry, 2010, 398(2): 885. sup. 893.); the silk-screen printing method is to coat the solid wax on the surface of the filter paper by adopting the silk-screen printing technology, then heat the filter paper, and form a corresponding pattern after the wax permeates into the filter paper after heating (W Dungchai, O Chailapakul, CS Henry. A low-cost, simple, and rapid fabrication method for paper-based microfluidics using wax screen-printing. analysis 2011, 136(1): 77-82.).

The manufacturing methods are high in cost, and the photoetching method needs photoresist; wax printing requires a specialized wax printer; ink-jet printing requires modification of the printer and also requires specially made inks; screen printing requires special dies. In addition, some substances are volatile or toxic, and influence the health of printing personnel in a high-concentration environment. The laser printing method of the present invention is thus proposed.

Disclosure of Invention

The invention provides a method for printing and preparing a microporous plate based on an office laser printer.

The technical scheme of the invention is as follows:

a preparation method of a paper microporous plate based on laser printing is characterized by comprising the following steps:

1) manufacturing a template: designing corresponding templates according to the aperture and the hole spacing of a commercial 96-hole micro-porous plate by using mapping software (such as photoshop);

2) printing: hydrophilic filter paper such as cellulose filter paper is used as a raw material, a printer is used for printing, a printed black area is a hydrophobic area, and a white area is a hydrophilic area. As shown in fig. 1. The printer is a conventional office laser printer. The toner cartridge used by the printer is an original toner cartridge. The original selenium drum comprises copolymer mixture (styrene and acrylate), wax, silicon dioxide and pigment.

In the step 1), the distance between holes in the template is 2mm, the diameter of the holes is 6mm, and the rest parts except the inner parts of the holes are black.

In the step 2), the printing mode is front-back alignment double-sided printing.

And 2) after the printing in the step 2) is finished, treating the filter paper at 150 ℃ for 2 hours, and taking out and cooling to room temperature.

The hydrophobic principle adopted by the invention is as follows: the cartridge is composed of copolymer mixture (styrene and acrylate), wax, silica and pigment. Waxes are water-insoluble substances, the presence of silica increasing their hydrophobicity. After printing and baking, the areas surrounded by wax form hydrophobic rings, and the areas without wax maintain the hydrophilicity of the filter paper.

In the invention, the laser printer with the original selenium drum is used for preparing the paper microporous plate for the first time, so the method has the following technical effects:

1) the preparation is simple, convenient and quick, the conventional printing can be finished without any treatment and special instruments, and the mass production can be realized;

2) the manufacturing cost is low, and only a laser printer for office is needed;

3) the operation of training professionals is not needed;

4) is safe and harmless, does not need to prepare any reagent, and has no toxic and harmful effects on the bodies of operators.

Drawings

FIG. 1 is a template pattern of a design;

FIG. 2 is a paper microporous plate prepared after front and back alignment printing;

FIG. 3 is contact angle data of hydrophobic regions of filter paper printed 1 times on a non-original cartridge;

FIG. 4 is a graph showing the leakage of hydrophilic pores in filter paper printed 1 time on a non-original cartridge;

FIG. 5 is contact angle data of the hydrophobic area of the filter paper printed 1 times on the original selenium drum;

FIG. 6 shows the leakage of hydrophilic pores on filter paper printed 1 time on the original cartridge;

FIG. 7 is contact angle data of hydrophobic areas of filter paper printed 2 times on an original selenium drum;

FIG. 8 is the leakage of the hydrophilic pores of the filter paper printed 2 times on the original cartridge;

FIG. 9 is contact angle data of hydrophobic areas of filter paper printed 3 times on an original selenium drum;

FIG. 10 shows the leakage of hydrophilic pores on filter paper printed 3 times on the original cartridge.

Detailed Description

Example 1

The preparation method of the paper microporous plate based on laser printing comprises the following steps:

firstly, a pattern of a required template is designed by utilizing photoshop drawing software, the diameter of a circle is 6mm, and the distance between two circles is 2 mm. The template of the software design is shown in fig. 1.

And secondly, printing the filter paper on the front and back sides of the non-original selenium drum in the printer for 1 time respectively. After printing, the filter paper is placed at 150 ℃ for 2 hours, and after natural cooling, the paper microporous plate is shown in fig. 2.

The contact angle is detected by dripping water in the black hydrophobic area, the result is shown in figure 3, the contact angle is 85.5 degrees (less than 90 degrees represents that the water is hydrophilic), and the black area has no hydrophobicity; at the same time, 50 microliter of dye was added dropwise to the white hydrophilic wells to observe the leakage. As a result, as shown in FIG. 4, it can be clearly seen that the dye is present in the peripheral holes of the dye dropping hole within about 1min, i.e., the microporous plate has poor sealing property and leakage.

The results show that the non-original selenium drum is not suitable for preparing the paper microporous plate.

Example 2

And secondly, printing the filter paper on the front and back sides of the original selenium drum in the printer for 1 time respectively. After printing, the filter paper was treated at 150 ℃ for 2 hours, and after natural cooling, the paper microplates were as shown in fig. 2.

The contact angle is detected by dropping water on the black hydrophobic area, and the result is shown in fig. 5, the contact angle is 100 ° (the contact angle is more than 90 °, which represents the hydrophobicity, the larger the contact angle is, the better the hydrophobicity is), which indicates that the black area has the hydrophobicity; at the same time, 50 microliter of dye is dripped into the white hydrophilic hole, and the sealing condition of the micropore is observed after half an hour. The result is shown in fig. 6, no dye appears in the holes around the dye dropping hole, i.e. the printing prepared microporous plate has no leakage.

The result shows that the micropore prepared by printing the original selenium drum has good sealing property.

Example 3

And secondly, the original selenium drum is arranged in the printer, and the filter paper is aligned to the front side and the back side and is printed for 2 times respectively. After printing, the filter paper was treated at 150 ℃ for 2 hours, and after natural cooling, the paper microplates were as shown in fig. 2.

Dropping water in the black hydrophobic area to detect the contact angle, wherein the data is shown in fig. 7, the contact angle is 113.3 degrees, which indicates that the black area has hydrophobicity; meanwhile, 50 microliter of dye is dripped into the white hydrophilic hole, and the dye leakage condition is observed after half an hour, so that the result is shown in figure 8, the color of the dye does not appear in the holes around the dripped dye hole, namely the microporous plate does not leak.

The results show that the micropore prepared by printing the original selenium drum for 2 times has good sealing performance and stronger hydrophobic effect than that of printing for 1 time.

Example 4

And secondly, the original selenium drum is arranged in the printer, and the filter paper is aligned to the front side and the back side and is printed for 3 times respectively. After printing, the filter paper was treated at 150 ℃ for 2 hours, and after natural cooling, the paper microplates were as shown in fig. 2.

Dropping water in the black hydrophobic area to detect the contact angle, wherein the data is shown in fig. 9, the contact angle is 104.7 degrees, which indicates that the black area has hydrophobicity; meanwhile, 50 microliter of dye is dripped into the white hydrophilic hole, and the dye leakage condition is observed after half an hour, so that the result is shown in figure 10, the color of the dye does not appear in the holes around the dripped dye hole, namely the microporous plate does not leak.

The results show that the micropore prepared by printing the original selenium drum for 3 times has good sealing performance and the hydrophobic effect is similar to that of printing for 2 times.

Claims

1. A preparation method of a paper microporous plate based on laser printing is characterized by comprising the following steps:

1) manufacturing a template: designing corresponding templates according to the aperture and the hole spacing of a commercial 96-hole micro-porous plate by using mapping software;

2) printing: using hydrophilic filter paper as a raw material, and printing by using a laser printer, wherein a printed black area is a hydrophobic area, and a white area is a hydrophilic area; the selenium drum used by the laser printer is an original selenium drum which mainly comprises styrene, acrylate, wax, silicon dioxide and pigment;

in the step 2), the printing mode is front-back alignment double-sided printing;

2. The method of claim 1, wherein: in the step 1), the distance between holes in the template is 2mm, the diameter of the holes is 6mm, and the rest parts except the inner parts of the holes are black.