CN106093027A - Heavy metal ion detection chip - Google Patents
Heavy metal ion detection chip Download PDFInfo
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- CN106093027A CN106093027A CN201610580067.6A CN201610580067A CN106093027A CN 106093027 A CN106093027 A CN 106093027A CN 201610580067 A CN201610580067 A CN 201610580067A CN 106093027 A CN106093027 A CN 106093027A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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Abstract
This application discloses a kind of heavy metal ion detection chip, including hydrophilic base, this substrate surface is coated with one layer of hydrophobic layer, this hydrophobic layer stops that heavy metal ion enters base material and organic solvent can be allowed to pass through, and described hydrophobic layer surface adsorption has can be with the developer of at least one heavy metal ion generation chromogenic reaction.In the detection method of the heavy metal ion of the micro-fluidic scraps of paper of the present invention, contents of many kinds of heavy metal ion gives birth to chromogenic reaction with predetermined substance is counter, makes the detection sensitivity of heavy metal ion obtain great lifting by surface-hydrophobicized process.This micro-fluidic paper chip detection of heavy metal ion method can overcome the shortcomings such as tradition detection of heavy metal ion instrument is complicated, sample requirements is big, application is narrow, testing cost is high, it is possible to online qualitative and quantitative detection heavy metal ion.
Description
Technical field
The application relates to a kind of micro-fluidic paper chip detection technique, particularly relates to a kind of heavy metal ion detection chip.
Background technology
China about 50% earth surface water source is contaminated, and hundreds of organic compound, heavy metal ion enter water source, simultaneously
Lower water source also exceeds standard at fluorine, arsenic, ferrum, manganese etc..Water containing heavy metal ion (cadmium, chromium, copper, hydrargyrum, nickel etc.) is drunk by the mankind, meeting
Causing the mankind to suffer from various disease, have even can be carcinogenic.It is that water environment causes heavy metal ion dirty that water heavy metal ion is polluted
The occurring source of dye.Cellulose is as a kind of natural water wetted material, and the dropping of aqueous testing sample can be in capillary in paper chip
The lower automatic flowing of effect, it is not necessary to external driver device, is a kind of extraordinary micro-fluidic material.Micro-fluidic paper chip has very
Being widely applied, in quick diagnosis, food safety, the aspect such as environment measuring plays a very important role.Through last decade
Development, micro-fluidic paper chip has many processing methods, such as cut paper method, wax seal method, impact system etc..Current detection means side
There are colorimetric detection method, Electrochemical Detection, chemiluminescence detection etc. in face.Utilize the water-wet behavior of paper chip, by hydrophobic material, photoetching
Glue, AKD, wax, PDMS, OTS, hydrogel patterning is imprinted on its surface, by constructing hydrophobic channel, Ke Yi in paper chip
Micro-fluidic paper chip is designed and produced in two dimensional surface.By by the paper chip stacked in multi-layers of two, or by the method for paper folding
Paper chip is rolled at one piece, and corresponding hydrophilic region is directed at UNICOM, the paper chip on three directions can be made.Due to many
Several methods for mass making paper chip construct the microchannel majority not organic solvent-resistant of paper chip, therefore limit refill
The sheet application when detection.
Micro-fluidic paper chip is a kind of emerging microfluidic analysis technology platform, have low cost, processing is simple, use and
The advantage such as easy to carry, controls and the application such as environmental monitoring has great application prospect at clinical diagnosis, food quality.
But the most frequently used determination method of traditional micro-fluidic paper chip is colorimetry, but to there is detection sensitivity low for colorimetry
Shortcoming.
Summary of the invention
It is an object of the invention to provide a kind of heavy metal ion detection chip, to overcome deficiency of the prior art.
For achieving the above object, the present invention provides following technical scheme:
The embodiment of the present application discloses a kind of heavy metal ion detection chip, and including hydrophilic base, this substrate surface covers
Being stamped one layer of hydrophobic layer, this hydrophobic layer stops that heavy metal ion enters base material and organic solvent can be allowed to pass through, described hydrophobic layer
Surface adsorption has can be with the developer of at least one heavy metal ion generation chromogenic reaction.
Preferably, in above-mentioned heavy metal ion detection chip, described base material is selected from filter paper, cellulose membrane, glass fibers
Dimension, writing paper or kraft paper.
Preferably, in above-mentioned heavy metal ion detection chip, described hydrophobic layer material selected from photoresist, AKD, wax,
PDMS or OTS.
Preferably, in above-mentioned heavy metal ion detection chip, the preparation method of described hydrophobic layer includes: by hydrophobic material
Material drips on the surface of micro-fluidic paper chip with the mixed solution of diluent, treat hydrophobic material be uniformly dispersed at substrate surface or
After coating uniformly, base material is heated so that hydrophobic material forms hydrophobic layer on surface..
Preferably, in above-mentioned heavy metal ion detection chip, described diluent is selected from the alcohol of a carbon to four carbon
Class, acetone, dichloromethane, hexane, heptane or petroleum ether.
Preferably, in above-mentioned heavy metal ion detection chip, described developer and metal ion Cr6+、Cu2+、Al3+、
Pb2+In at least one chromogenic reaction can occur.
Compared with prior art, it is an advantage of the current invention that: the detection side of the heavy metal ion of the micro-fluidic scraps of paper of the present invention
In method, contents of many kinds of heavy metal ion gives birth to chromogenic reaction with predetermined substance is counter, makes heavy metal ion by surface-hydrophobicized process
Detection sensitivity has obtained great lifting.This micro-fluidic paper chip detection of heavy metal ion method can overcome tradition heavy metal
The shortcomings such as ion detection instrument is complicated, sample requirements is big, application is narrow, testing cost is high, it is possible to online qualitative and quantitative
Detection heavy metal ion.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present application or technical scheme of the prior art, below will be to embodiment or existing
In having technology to describe, the required accompanying drawing used is briefly described, it should be apparent that, the accompanying drawing in describing below is only this
Some embodiments described in application, for those of ordinary skill in the art, on the premise of not paying creative work,
Other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 show the top view of heavy metal ion detection chip in the specific embodiment of the invention；
Fig. 2 show the side view of heavy metal ion detection chip in the specific embodiment of the invention；
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out detailed retouching
State, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based on the present invention
In embodiment, the every other enforcement that those of ordinary skill in the art are obtained on the premise of not making creative work
Example, broadly falls into the scope of protection of the invention.
Shown in Fig. 1 and Fig. 2, heavy metal ion detection chip, including base material, this base material is formed with the interior of hydrophiling
Portion's base material 1, the surface 2 of hydrophobization, surface adsorption has developer 3, and on the surface 2 of hydrophobization, definition has chromogenic reaction generation area
4。
In this technical scheme, hydrophiling inner substrate storage developer, hydrophobizing surface adsorbs a certain amount of developer, and
Stop heavy metal ion to be measured internal deeply toward base material so that chromogenic reaction occurs in micro-fluidic paper chip surface, chromogenic reaction
The region that generation area reacts with developer as heavy metal ion solution to be measured.
In above-mentioned heavy metal ion detection chip, the base material that can select has filter paper, cellulose membrane, glass fibre, book
Write paper using, kraft paper.
In above-mentioned heavy metal ion detection chip, the material that the hydrophobization of described micro-fluidic paper chip processes can be
Photoresist, AKD, wax, PDMS, OTS etc., the most various hydrophobic materials can select a series of concentration proportioning with diluent
(0.01%-10%).
In above-mentioned heavy metal ion detection chip, by the mixed solution 1 of the hydrophobic material of appropriate volume Yu diluent
～2 μ l drip on the surface of micro-fluidic paper chip.Conventional diluent has the carbon alcohols to four carbon, acetone, dichloromethane
Alkane, hexane, heptane, petroleum ether etc..
Heavy metal ion solution to be measured can be enriched in surface by the hydrophobic material on micro-fluidic surface.
In above-mentioned heavy metal ion detection chip, the above-mentioned hydrophobic material of solid block, powder, film like can be selected
Material is micro-fluidic paper chip surface-coated one layer.
After hydrophobic material is uniform at micro-fluidic paper chip Dispersion on surface or coating is uniform, micro-fluidic paper chip is put into
In baking oven or carry out heating 100～150 DEG C on hot plate, heat time heating time 2～3min so that hydrophobic material is formed on surface
Hydrophobic interfaces layer.
The detection method utilizing above-mentioned detection chip includes: developer dissolves in organic solvent, developer dropping is existed
The surface of micro-fluidic paper chip, by capillarity by chromogenic reagent solution absorption in the surface of base material and inside.Treat that solvent volatilizees
After, the dropping of band check weighing metal ion solution is reacted in chromogenic reaction generation area 4, the heavy metal ion that can be detected
There is Cr6+、Cu2+、Al3+、Pb2+One or more of.
Developer is soluble in multiple organic solvent such as the alcohols of carbon to four carbon, acetone, dichloromethane, oneself
Alkane, heptane, petroleum ether etc..
Heavy metal ion is reacted with developer, and the color product that reaction generates is gathered in the print that heavy metal ion solution stays
At note.
In one embodiment, the AKD-n-heptane solution 15 μ l of 0.1% is added in micro-fluidic paper chip hydrophobization
Surface 2, solution diffuses to whole micro-fluidic paper chip region 1,2 and 4 under capillary action, after normal heptane volatilizees, by miniflow
Control paper chip is put into and is incubated 2～3min at 100 DEG C.The micro-fluidic paper chip made is taken out, in chromogenic reaction generation area 4
Dripping 0.5 μ l concentration is the DPC of 3.5%, cupferron, dithizone, chrome azurol S acetone soln.After carrying liqs volatilization, check weighing will be treated
Metal ion solution drips in chromogenic reaction generation area 4, and the metal ion that can be detected has Cr6+、Cu2+、Al3+、Pb2+It
One or more, corresponding Monitoring lower-cut is respectively 0.05mg/L, 0.1mg/L, 0.01mg/L, 0.005mg/L, respectively lower than country
Related request in standard.
Here, also, it should be noted in order to avoid having obscured the present invention because of unnecessary details, the most only
Show and according to the closely-related structure of the solution of the present invention and/or process step, and eliminate little with relation of the present invention
Other details.
Finally, in addition it is also necessary to explanation, term " includes ", " comprising " or its any other variant are intended to non-exclusive
Comprising of property, so that include that the process of a series of key element, method, article or equipment not only include those key elements, and
Also include other key elements being not expressly set out, or also include intrinsic for this process, method, article or equipment
Key element.
Claims (6)
1. a heavy metal ion detection chip, it is characterised in that include hydrophilic base, this substrate surface is coated with one layer and dredges
Water layer, this hydrophobic layer stops that heavy metal ion enters base material and organic solvent can be allowed to pass through, and described hydrophobic layer surface adsorption has
Can be with the developer of at least one heavy metal ion generation chromogenic reaction.
Heavy metal ion detection chip the most according to claim 1, it is characterised in that: described base material is selected from filter paper, fiber
Element film, glass fibre, writing paper or kraft paper.
Heavy metal ion detection chip the most according to claim 1, it is characterised in that: described hydrophobic layer material is selected from photoetching
Glue, AKD, wax, PDMS or OTS.
Heavy metal ion detection chip the most according to claim 1, it is characterised in that: the preparation method bag of described hydrophobic layer
Include: the mixed solution of hydrophobic material with diluent is dripped on the surface of micro-fluidic paper chip, treats that hydrophobic material is at substrate surface
After being uniformly dispersed or coating uniformly, base material is heated so that hydrophobic material forms hydrophobic layer on surface..
Heavy metal ion detection chip the most according to claim 4, it is characterised in that: described diluent is selected from a carbon extremely
The alcohols of four carbon, acetone, dichloromethane, hexane, heptane or petroleum ether.
Heavy metal ion detection chip the most according to claim 1, it is characterised in that: described developer and metal ion Cr6 +、Cu2+、Al3+、Pb2+In at least one chromogenic reaction can occur.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610580067.6A CN106093027A (en) | 2016-07-22 | 2016-07-22 | Heavy metal ion detection chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610580067.6A CN106093027A (en) | 2016-07-22 | 2016-07-22 | Heavy metal ion detection chip |
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| CN106093027A true CN106093027A (en) | 2016-11-09 |
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| CN201610580067.6A Pending CN106093027A (en) | 2016-07-22 | 2016-07-22 | Heavy metal ion detection chip |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108872081A (en) * | 2018-09-04 | 2018-11-23 | 重庆科技学院 | A kind of multi-layer micro-fluidic chips detecting heavy metal ion |
| CN109142299A (en) * | 2018-09-08 | 2019-01-04 | 重庆科技学院 | A kind of petroleum wastewater heavy metal ion detection chip application method |
| CN109297867A (en) * | 2018-10-15 | 2019-02-01 | 成都理工大学 | A kind of contaminated area field quick detection pipe and detection method |
| CN109752231A (en) * | 2017-11-02 | 2019-05-14 | 浙江清华长三角研究院 | One kind being used for Cr(VI) it is enriched with the micro-fluidic chip of detection |
| CN110579469A (en) * | 2019-09-29 | 2019-12-17 | 桂林理工大学 | Instrument-free quantitative detection method for divalent mercury ions |
| CN116500099A (en) * | 2023-03-31 | 2023-07-28 | 宿州学院 | Microfluidic sensor for rapidly detecting heavy metal ions and application method thereof |
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| US20120238008A1 (en) * | 2011-03-15 | 2012-09-20 | Colorado State University Research Foundation | Rapid Detection of Pathogens Using Paper Devices |
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| CN103105392A (en) * | 2013-01-20 | 2013-05-15 | 桂林理工大学 | Production method and application of patterned biochemical analysis test paper |
| CN103575728A (en) * | 2012-08-01 | 2014-02-12 | 中国科学院大连化学物理研究所 | Trace heavy metal ion detection method taking filter paper as basic colorimetric sensor array |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109752231A (en) * | 2017-11-02 | 2019-05-14 | 浙江清华长三角研究院 | One kind being used for Cr(VI) it is enriched with the micro-fluidic chip of detection |
| CN108872081A (en) * | 2018-09-04 | 2018-11-23 | 重庆科技学院 | A kind of multi-layer micro-fluidic chips detecting heavy metal ion |
| CN109142299A (en) * | 2018-09-08 | 2019-01-04 | 重庆科技学院 | A kind of petroleum wastewater heavy metal ion detection chip application method |
| CN109142299B (en) * | 2018-09-08 | 2023-07-18 | 重庆科技学院 | Application method of petroleum wastewater heavy metal ion detection chip |
| CN109297867A (en) * | 2018-10-15 | 2019-02-01 | 成都理工大学 | A kind of contaminated area field quick detection pipe and detection method |
| CN109297867B (en) * | 2018-10-15 | 2023-10-13 | 成都理工大学 | On-site rapid detection tube and detection method for polluted area |
| CN110579469A (en) * | 2019-09-29 | 2019-12-17 | 桂林理工大学 | Instrument-free quantitative detection method for divalent mercury ions |
| CN116500099A (en) * | 2023-03-31 | 2023-07-28 | 宿州学院 | Microfluidic sensor for rapidly detecting heavy metal ions and application method thereof |
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Application publication date: 20161109 |