CN103389303A - Multi-index analysis paper chip and preparation method thereof - Google Patents
Multi-index analysis paper chip and preparation method thereof Download PDFInfo
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- CN103389303A CN103389303A CN2013103417765A CN201310341776A CN103389303A CN 103389303 A CN103389303 A CN 103389303A CN 2013103417765 A CN2013103417765 A CN 2013103417765A CN 201310341776 A CN201310341776 A CN 201310341776A CN 103389303 A CN103389303 A CN 103389303A
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Abstract
The invention provides a multi-index analysis paper chip and a preparation method thereof. The multi-index analysis paper chip is characterized by being prepared by designing hydrophobic patterns on the surface of a hydrophilic paper sheet or polymeric membrane. The preparation method comprises the following steps of: (1) carrying out hydrophobic modification on the hydrophilic paper sheet or polymeric membrane by using a vapor deposition technology; (2) clinging a mask with an array through hole structure to the surface of the paper chip, and carrying out dry etching by using etching gas to obtain a hydrophilic array detecting region; and (3) fixing a chemical color reagent or biological probe in a hydrophilic detecting point. The preparation method of the paper chip provided by the invention is very rapid, simple and convenient, and multi-index on-site real-time detection can be realized. The preparation method is particularly suitable for producing disposable paper chips in a large scale, and is expected to be applied to biochemical medical diagnosis, environment monitoring and safe and rapid food detection.
Description
Technical field
The present invention relates to the micro-fluidic chip field, particularly relate to a kind of many index analysis refill sheet and preparation method thereof.
Background technology
The refill sheet refers to carry out a kind of test paper chip of chemistry, bioanalysis in the substrate of paper or polymeric membrane, due to one-shot forming, convenient and swift, with low cost, be very suitable for the making of disposable multiple biochemical analysis test paper, therefore, many index analysis refill sheet is also referred to as micro-fluidic chip on paper (paper-based microfluidic chips).At present, the refill sheet is widely used as a kind of conveniently analysis means in actual life, such as common pH test paper, urine detection test paper.But present refill sheet index is single, the preparation slightly complicated, and such as making the material such as SU8 or dimethyl silicone polymer (PDMS) with photoresist, cost is expensive and uncommon, is unfavorable for the application of patterning analysis test paper.The method of the material that the present invention adopts this low cost of silicon fluoride that gas phase modifies, be easy to get replaces the preparation method of the materials such as photoresist SU8 or dimethyl silicone polymer (PDMS), greatly reduced cost, simplified operation steps, provide very simple and easy to do method for patterned biochemical analysis test paper really is applied to actual production, tool is of great significance.
Summary of the invention
The invention provides a kind of many index analysis refill sheet and preparation method thereof, it is characterized in that this chip is to be prepared from by the hydrophobic pattern of the surface construction at hydrophilic paper or polymeric membrane.Described paper comprises filter paper, thieving paper etc.; Described polymeric membrane comprises nitrocellulose filter, glass fibre membrane etc.
The invention provides a kind of many index analysis refill sheet and preparation method thereof, it is characterized in that, have a plurality of check points that are fixed with chemochromic reagent or bioprobe on described many index analysis refill sheet.
The invention provides a kind of many index analysis refill sheet and preparation method thereof, it is characterized in that, the preparation method of described refill sheet comprises the following steps:
1. use gas phase deposition technology to carry out water wettability paper or polymeric membrane hydrophobically modified;
2. a mask plate with array through-hole structure is close to refill sheet surface, uses etching gas to carry out dry etching, obtain hydrophilic array detection zone;
3. fixedly chemochromic reagent or bioprobe in hydrophilic check point.
While adopting described refill sheet to carry out many index analysis, sample solution is joined different check points, can carry out many index determinings according to principles such as chemical colour reaction reaction or biological immune reaction, nucleic acid hybridizations.
Description of drawings
Fig. 1. the invention provides a kind of preparation method of many index analysis refill sheet.Wherein, A is the hydrophilic paper substrate; B is for modifying the refill sheet of silicon fluoride; C is the oxygen plasma etching based on mask; D is the refill sheet of hydrophilic-hydrophobic pattern; E is the refill sheet that is fixed with chemochromic reagent or bioprobe.
Fig. 2. the structural representation of a kind of many index analysis of array refill sheet provided by the invention.
Fig. 3. a kind of micro-fluidic refill sheet that is connected check point by a public injection port and a plurality of hydrophilic pathway provided by the invention.
Specific embodiments
The following examples are further described the present invention in connection with Figure of description.
Structure and the preparation thereof of 1 one kinds of many index analysis of array refill sheets of embodiment
A kind of many index analysis of array refill sheet, surface has the circle detection dot structure of array, and its preparation method is as shown in Figure l.At first the method for using gas phase to modify, carry out the modification of silicon fluoride (17 fluorine decyl trimethoxy silane) on Whatman filter paper A surface, prepare surface and be hydrophobic refill sheet B; A thickness lmm, the dimethyl silicone polymer film that is processed with array manhole structure are close on refill sheet B, are placed in oxygen plasma and carry out dry etching except the glue machine, at hydrophobic B chip surface, prepare hydrophilic circle detection zone D; The last solution that drips chemochromic reagent or bioprobe in this zone, such as metallic ion developer, protein probe etc., after dry solidification, prepare many index analysis refill sheet E of array.
As shown in Figure 2, during use, only the sample solution of certain volume need to be added drop-wise in check point, due to the edge hydrophobic effect, sample solution only is limited in check point.After standing a period of time, component to be measured in sample and the reagent reacting that is fixed on the refill sheet are abundant, chromogenic reaction or specific biologically will occur, and can distinguish or be placed in very easily special detecting device by human eye finally and carry out qualitative or quantitative analyzing and testing.
Structure and the preparation thereof of 2 one kinds of micro-fluidic many index analysis refill sheets of embodiment
As shown in Figure 3, a kind of micro-fluidic many index analysis refill sheet, connect check point by a public injection port and a plurality of hydrophilic pathway and form.Preparation process in its preparation method and embodiment 1 is similar, difference is, for obtain as shown in Figure 3 hydrophilic-the hydrophobic pattern figure, use a dimethyl silicone polymer film that is processed with the full engraved structure of manhole and fluid passage to be close on refill sheet B, be placed in oxygen plasma and carry out dry etching except the glue machine, the final micro-fluidic refill sheet that obtains to contain a plurality of check points.
During use, only the sample solution of certain volume need to be added drop-wise in public injection port, due to the water-wet behavior of edge hydrophobic effect and fluid passage, sample solution will enter check point along fluid passage.After standing a period of time, component to be measured in sample and the reagent reacting that is fixed in check point are abundant, chromogenic reaction or specific biologically will occur, and can distinguish or be placed in very easily special detecting device by human eye finally and carry out qualitative or quantitative analyzing and testing.
Refill sheet provided by the present invention, the preparation method is very fast and convenient, and can realize on-the-spot instant detection of many indexs.Be particularly suitable for producing in batches the disposable paper chip, be expected to be applied to biochemical medical diagnosis, environmental monitoring, food security fast detecting.
Claims (4)
1. sheet of index analysis refill more than a kind and preparation method thereof, is characterized in that this chip is to be prepared from by the hydrophobic pattern of the surface construction at hydrophilic paper or polymeric membrane.
2., according to a kind of many index analysis refill sheet claimed in claim 1 and preparation method thereof, it is characterized in that having a plurality of check points that are fixed with chemochromic reagent or bioprobe on described many index analysis refill sheet.
3., according to a kind of many index analysis refill sheet claimed in claim 1 and preparation method thereof, it is characterized in that, the preparation method of described refill sheet comprises the following steps:
1. use gas phase deposition technology to carry out water wettability paper or polymeric membrane hydrophobically modified;
2. a mask plate with array through-hole structure is close to refill sheet surface, uses etching gas to carry out dry etching, obtain hydrophilic array detection zone;
3. fixedly chemochromic reagent or bioprobe in hydrophilic check point.
4. according to a kind of many index analysis refill sheet claimed in claim 1 and preparation method thereof, it is characterized in that, while adopting described refill sheet to carry out many index analysis, sample solution is joined different check points, can carry out many index determinings according to principles such as chemical colour reaction reaction or biological immune reaction, nucleic acid hybridizations.
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104483360A (en) * | 2014-12-08 | 2015-04-01 | 中国科学院烟台海岸带研究所 | Potential paper chip and preparation and application thereof |
| CN104535563A (en) * | 2014-12-25 | 2015-04-22 | 浙江大学 | Detection system for spraying droplet density and size capable of being well used under both dry and wet environments, preparation method and detection method |
| CN105527282A (en) * | 2014-10-16 | 2016-04-27 | 郑兆珉 | Cloth-based biochemical detection device and manufacturing method thereof |
| CN105954524A (en) * | 2016-07-15 | 2016-09-21 | 陶少强 | Biochip for human heat shock protein detection |
| CN106018786A (en) * | 2016-07-15 | 2016-10-12 | 陶少强 | Intelligent detection device for detecting procalcitonin |
| CN106485250A (en) * | 2016-09-09 | 2017-03-08 | 华东理工大学 | A kind of recognition methods of paper chip gray value and its application |
| CN106807462A (en) * | 2017-01-13 | 2017-06-09 | 湖南师范大学 | A kind of acid etch modifies the preparation method of micro-fluidic paper chip |
| CN107384769A (en) * | 2017-08-24 | 2017-11-24 | 李峰 | Nucleic acid detection apparatus and its application based on paper |
| CN107462571A (en) * | 2016-06-02 | 2017-12-12 | 中国科学院理化技术研究所 | Preparation method and application of paper-based hydrophobic isolation pattern |
| US10106832B2 (en) | 2015-09-01 | 2018-10-23 | National Tsing Hua University | Method for manufacturing a microbial detection device, microbial detection method, microbial detection kit, and microbial detection device |
| CN109136077A (en) * | 2018-08-20 | 2019-01-04 | 中南大学 | A kind of preparation method of biochip |
| CN109580489A (en) * | 2017-09-29 | 2019-04-05 | 深圳先进技术研究院 | The chip and method of blood glucose and blood lipid are detected simultaneously |
| CN110157607A (en) * | 2019-06-03 | 2019-08-23 | 中国农业科学院农田灌溉研究所 | Soil microbial community characteristic reaction device and measurement device |
| CN110205236A (en) * | 2019-06-12 | 2019-09-06 | 中国检验检疫科学研究院 | A kind of paper micro-fluidic chip quickly detecting nucleic acid based on RPA technology |
| CN110231478A (en) * | 2019-07-03 | 2019-09-13 | 杭州霆科生物科技有限公司 | A kind of residual detection chip of agriculture of integrated enzyme inhibition and immuno-array |
| CN111269799A (en) * | 2020-02-12 | 2020-06-12 | 赛纳生物科技(北京)有限公司 | Preparation method of oil-sealed biochemical chip |
| CN111965095A (en) * | 2020-06-28 | 2020-11-20 | 中汽数据有限公司 | Environmental corrosion factor detection system and detection method |
| CN117233395A (en) * | 2023-09-28 | 2023-12-15 | 上海交通大学 | Quick diagnosis chip based on liquid unidirectional rectification and manufacturing method thereof |
| CN117330552A (en) * | 2023-11-27 | 2024-01-02 | 中国科学院烟台海岸带研究所 | Method for detecting gondolac toxins 1,4 by using molecular imprinting sensing paper chip |
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Cited By (25)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105527282A (en) * | 2014-10-16 | 2016-04-27 | 郑兆珉 | Cloth-based biochemical detection device and manufacturing method thereof |
| CN104483360A (en) * | 2014-12-08 | 2015-04-01 | 中国科学院烟台海岸带研究所 | Potential paper chip and preparation and application thereof |
| CN104535563A (en) * | 2014-12-25 | 2015-04-22 | 浙江大学 | Detection system for spraying droplet density and size capable of being well used under both dry and wet environments, preparation method and detection method |
| US10106832B2 (en) | 2015-09-01 | 2018-10-23 | National Tsing Hua University | Method for manufacturing a microbial detection device, microbial detection method, microbial detection kit, and microbial detection device |
| CN107462571A (en) * | 2016-06-02 | 2017-12-12 | 中国科学院理化技术研究所 | Preparation method and application of paper-based hydrophobic isolation pattern |
| CN107462571B (en) * | 2016-06-02 | 2020-04-24 | 中国科学院理化技术研究所 | Preparation method and application of paper-based hydrophobic isolation pattern |
| CN106018786B (en) * | 2016-07-15 | 2018-06-29 | 安徽国科生物科技有限公司 | For detecting the intelligent detection equipment of Procalcitonin |
| CN106018786A (en) * | 2016-07-15 | 2016-10-12 | 陶少强 | Intelligent detection device for detecting procalcitonin |
| CN105954524A (en) * | 2016-07-15 | 2016-09-21 | 陶少强 | Biochip for human heat shock protein detection |
| CN106485250A (en) * | 2016-09-09 | 2017-03-08 | 华东理工大学 | A kind of recognition methods of paper chip gray value and its application |
| CN106807462A (en) * | 2017-01-13 | 2017-06-09 | 湖南师范大学 | A kind of acid etch modifies the preparation method of micro-fluidic paper chip |
| CN106807462B (en) * | 2017-01-13 | 2019-05-10 | 湖南师范大学 | A kind of acid etch modifies the preparation method of micro-fluidic paper chip |
| CN107384769A (en) * | 2017-08-24 | 2017-11-24 | 李峰 | Nucleic acid detection apparatus and its application based on paper |
| CN107384769B (en) * | 2017-08-24 | 2021-04-09 | 李峰 | Paper-based nucleic acid detection device and application thereof |
| CN109580489A (en) * | 2017-09-29 | 2019-04-05 | 深圳先进技术研究院 | The chip and method of blood glucose and blood lipid are detected simultaneously |
| CN109136077A (en) * | 2018-08-20 | 2019-01-04 | 中南大学 | A kind of preparation method of biochip |
| CN110157607A (en) * | 2019-06-03 | 2019-08-23 | 中国农业科学院农田灌溉研究所 | Soil microbial community characteristic reaction device and measurement device |
| CN110205236A (en) * | 2019-06-12 | 2019-09-06 | 中国检验检疫科学研究院 | A kind of paper micro-fluidic chip quickly detecting nucleic acid based on RPA technology |
| CN110231478A (en) * | 2019-07-03 | 2019-09-13 | 杭州霆科生物科技有限公司 | A kind of residual detection chip of agriculture of integrated enzyme inhibition and immuno-array |
| CN111269799A (en) * | 2020-02-12 | 2020-06-12 | 赛纳生物科技(北京)有限公司 | Preparation method of oil-sealed biochemical chip |
| CN111269799B (en) * | 2020-02-12 | 2023-07-04 | 赛纳生物科技(北京)有限公司 | Preparation method of oil-sealed biochemical chip |
| CN111965095A (en) * | 2020-06-28 | 2020-11-20 | 中汽数据有限公司 | Environmental corrosion factor detection system and detection method |
| CN117233395A (en) * | 2023-09-28 | 2023-12-15 | 上海交通大学 | Quick diagnosis chip based on liquid unidirectional rectification and manufacturing method thereof |
| CN117330552A (en) * | 2023-11-27 | 2024-01-02 | 中国科学院烟台海岸带研究所 | Method for detecting gondolac toxins 1,4 by using molecular imprinting sensing paper chip |
| CN117330552B (en) * | 2023-11-27 | 2024-02-02 | 中国科学院烟台海岸带研究所 | Method for detecting gondolac toxins 1,4 by using molecular imprinting sensing paper chip |
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Application publication date: 20131113 |