CN104209153A - Preparation method of paper micro-fluidic chips - Google Patents
Preparation method of paper micro-fluidic chips Download PDFInfo
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- CN104209153A CN104209153A CN201410457911.7A CN201410457911A CN104209153A CN 104209153 A CN104209153 A CN 104209153A CN 201410457911 A CN201410457911 A CN 201410457911A CN 104209153 A CN104209153 A CN 104209153A
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Abstract
The invention discloses a preparation method of paper micro-fluidic chips. The preparation method of the paper micro-fluidic chips comprises the following steps: step one, designing patterns of chips; step two, mixing methylbenzene with polydimethylsiloxane (PDMS) according to a mass ratio of (3:1)-(8:1), and stirring to obtain a mixed solution; and step three, injecting the mixed solution into an ink box of an ink-jet printer, printing the designed chip patterns on porous membranes by using the ink-jet printer, drying the porous membranes at the temperature of 70-90 DEG C for 1-3 hours after the printing is finished, taking the porous membranes out and cooling to obtain the micro-fluidic chips. The preparation method of the paper micro-fluidic chips is simple, feasible, low in cost and suitable for industrialization.
Description
Technical field
The present invention relates to chip field, especially relate to a kind of preparation method of papery micro-fluidic chip.
Background technology
Micro-fluidic (Microfluidics) or micro-total analysis system (MicroTAS), or the laboratory (Lab On a Chip) on chip is a kind of with accurate manipulation and control minute yardstick fluid, and especially submicrometer structure is the science and technology of principal character." laboratory on chip " refers to utilize microflow control technique by multiple system (as sample preparation, mixing, compartment analysis and detection) integrated concept on one chip.Microflow control technique is proposed in early 1990s first by Manz etc., microminiaturized by analytical instrument, drastically increases efficiency and the speed of compartment analysis.Nowadays, it has developed into the brand-new research field that multidisciplinary (engineering, physics, chemistry, micro Process and bioengineering) intersects, and for many analytical systems bring microminiaturized benefit.Micro-fluidic chip has following characteristics: less sample volume consumption, less energy ezpenditure, less device etc.
Papery micro-fluidic chip (Paper-based Microfluidics) is a kind of new microfluidic chip developed in recent years.Proposed papery micro-fluidic chip concept by the Whitesides group of Harvard University first in 2007 and produce papery chip, (Martinez A W is detected while the micro-fluidic chip made achieves bovine serum albumin(BSA) (BSA) and glucose, Phillips S T, Butte M J, et al. Patterned paper as a platform for inexpensive, low ?volume, portable bioassays [J]. Angewandte Chemie International Edition, 2007,46 (8): 1318-1320.).It utilizes paper as the substrate traditional microfluidic chip that to instead of with materials such as silicon, glass, high polymers be substrate.Micro-fluidic chip material is compared with common micro-fluidic chip, it is without the need to by outer Force system being the liquid flow in passage, it is that paper substrates processes through hydrophilic or hydrophobic processing by one, the region that hydrophilic paper material is divided into hydrophilic/hydrophobic alternate, form hydrophilic pathway, i.e. microfluidic channel.An important advantage of paper is exactly that liquid flow can not mix, and can flow in different passage, can not interference experiment result, so just can ensure many flux distributions.Compared with traditional silicon, glass, high polymer micro-flow control chips, papery micro-fluidic chip has the following advantages: cost is low, detection background is low, bio-compatibility is good, the easier portability of analytical system, post processing are simple etc.
The method of current making papery micro-fluidic chip comprises: photoetching process, ink-jet etching method, plasma processing, wax seal method, silk screen print method etc.Wherein, photolithography steps is loaded down with trivial details, and the optical cement reagent used is expensive, is unfavorable for batch production; In plasma processing method, plasma atmosphere easily penetrates into the area of coverage from vacancy section; The papery micro-fluidic chip that wax seal method makes has the shortcomings such as resolution ratio is low.
Summary of the invention
the technical problem solved:for prior art problem, the invention provides a kind of preparation method of papery micro-fluidic chip, technique is simple, with low cost, is applicable to large-scale production.
technical scheme:
A kind of preparation method of papery micro-fluidic chip:
The first step, the pattern of design chips;
Second step, is that 3:1-8:1 mix with dimethyl silicone polymer according to mass ratio by toluene, stirs to obtain mixed liquor;
3rd step, injects the print cartridge of ink-jet printer, utilizes ink-jet printer to be printed on porous membrane by designed chip design by above-mentioned mixed liquor, after printing completes, by dry for porous membrane 1-3h, temperature is 70-90 DEG C, take out cooling, obtain micro-fluidic chip.
As preferably, the first step, pattern has three identical raceway grooves, and the width of every bar raceway groove, length and surveyed area diameter are respectively 200 μm, 9mm and 1.5mm, and the live width of pattern is 1mm.
As preferably, dimethyl silicone polymer is that the monomer of 10:1 and initator mix by mass ratio.
As preferably, second step, the mass ratio of toluene and dimethyl silicone polymer is 8:1.
As preferably, the 3rd step, porous membrane is nitrocellulose membrane.
As preferably, the 3rd step, after having printed, porous membrane drying process 2h, temperature is 80 DEG C.
As preferably, the 3rd step, the nozzle temperature of printer and substrate temperature are 25 DEG C, print voltage 30-40V, dot spacing scope 10-80 μm, print number of plies 10-15 layer.
As preferably, the 3rd step, the dot spacing of printer 30 μm, prints the number of plies 10 layers.
The present invention is that the print cartridge mixed liquor of PDMS and toluene being injected printer serves as " ink ", and porous fiber film serves as " paper ", so the micro-fluidic chip of preparation is called papery micro-fluidic chip.
beneficial effect
The present invention first designs pattern, directly PDMS is printed on porous membrane, eliminate the complicated processes preparing model in the methods such as photoetching process, wax seal, serigraphy, Simplified flowsheet, reduces cost, without the need to specific template, gained chip resolution is high, and pattern can design many raceway grooves as required, be applicable to large-scale production, can be applicable to the other field of fast detection of bed.
Accompanying drawing explanation
Fig. 1 is the microfluidic chip structure schematic diagram of three raceway grooves prepared by the present invention, wherein, and 1. raceway groove, 2. surveyed area, 3. sample application zone;
Fig. 2 is the pictorial diagram by the chip hydrophily channel width prepared under microscopic examination different condition, and wherein, a is chip prepared by embodiment 1, and b is chip prepared by embodiment 2, and c is chip prepared by embodiment 3.
Detailed description of the invention
The following examples can make the present invention of those skilled in the art comprehend, but do not limit the present invention in any way.
Embodiment 1
The first step, utilizes Photoshop to design required chip design, and pattern is the width of three identical raceway grooves, every bar raceway groove, and length and surveyed area diameter are respectively 180 μm, 8mm and 1.2mm, and the live width of pattern is 0.8mm.
Second step, mixes toluene according to mass ratio 3:1 with PDMS, the mixed liquor of stirring, and wherein, PDMS is monomer: initator in mass ratio 10:1 mixes.
3rd step, syringe is utilized by above-mentioned mixed liquor (being commonly called as " ink ") to inject the supporting print cartridge of ink-jet printer by the filter tip that pore size is 0.22 μm, the nozzle temperature of setting printer is 25 DEG C, substrate temperature is 25 DEG C, printing voltage is 36V, dot spacing is 30 μm and prints 10 layers, after printing completes, baking oven printing being had the nitrocellulose membrane of PDMS " ink " to put into 70 DEG C processes 3 hours, naturally papery micro-fluidic chip is obtained after cooling, by gained chip detection, as shown in a in Fig. 2, the channel width of papery micro-fluidic chip is 155.635 μm.
Embodiment 2
The first step, utilizes Photoshop to design required chip design, and pattern is the width of five identical raceway grooves, every bar raceway groove, and length and surveyed area diameter are respectively 200 μm, 9mm and 1.5mm, and the live width of pattern is 1mm.
Second step, mixes toluene according to mass ratio 5:1 with PDMS, the mixed liquor of stirring, and wherein, PDMS is monomer: initator in mass ratio 10:1 mixes.
3rd step, syringe is utilized by above-mentioned mixed liquor (being commonly called as " ink ") to inject the supporting print cartridge of ink-jet printer by the filter tip that pore size is 0.22 μm, the nozzle temperature of setting printer is 25 DEG C, substrate temperature is 25 DEG C, printing voltage is 36V, dot spacing is 30 μm and prints 10 layers, after printing completes, baking oven printing being had the nitrocellulose membrane of PDMS " ink " to put into 80 DEG C processes 2 hours, naturally papery micro-fluidic chip is obtained after cooling, by gained chip detection, as shown in b in Fig. 2, the channel width of papery micro-fluidic chip is 170.283 μm.
Embodiment 3
The first step, utilizes Photoshop to design required chip design, and pattern is the width of five identical raceway grooves, every bar raceway groove, and length and surveyed area diameter are respectively 210 μm, 9.5mm and 1.8mm, and the live width of pattern is 1.2mm.
Second step, mixes toluene according to mass ratio 8:1 with PDMS, the mixed liquor of stirring, and wherein, PDMS is monomer: initator in mass ratio 10:1 mixes.
3rd step, syringe is utilized by above-mentioned mixed liquor (being commonly called as " ink ") to inject the supporting print cartridge of ink-jet printer by the filter tip that pore size is 0.22 μm, the nozzle temperature of setting printer is 25 DEG C, substrate temperature is 25 DEG C, printing voltage is 36V, dot spacing is 30 μm and prints 10 layers, after printing completes, baking oven printing being had the nitrocellulose membrane of PDMS " ink " to put into 90 DEG C processes 1 hour, naturally papery micro-fluidic chip is obtained after cooling, by gained chip detection, as shown in c in Fig. 2, the channel width of papery micro-fluidic chip is 214.227 μm.
Test philosophy: deposition detects antibody in optional two surveyed areas in chip prepared by the present invention after, dry process, testing sample solution is added in the circular sample application zone of chip, sample solution is in the dirty region after testing of the capillarity of nitrocellulose filter, now be deposited on surveyed area antibody can and testing sample solution in antigen combine, because the antigen in testing sample has set microballoon mark, so can obtain corresponding detection signal at surveyed area.
The hydrophily channel width of papery micro-fluidic chip will affect the resolution ratio of chip, and hydrophily raceway groove is narrower, and the resolution ratio of chip will be higher, be applied to testing sample amount required in detection fewer, realize low cost, the advantages such as portability.Can be found out by embodiment 1-3, not only width is little for gained chip raceway groove of the present invention, and the gap of setting width and developed width is also very little, is applicable to large-scale production.
Claims (8)
1. a preparation method for papery micro-fluidic chip, is characterized in that, comprises the following steps:
The first step, the pattern of design chips;
Second step, is that 3:1-8:1 mix with dimethyl silicone polymer according to mass ratio by toluene, stirs to obtain mixed liquor;
3rd step, injects the print cartridge of ink-jet printer, utilizes ink-jet printer to be printed on porous membrane by designed chip design by above-mentioned mixed liquor, after printing completes, by dry for porous membrane 1-3h, temperature is 70-90 DEG C, take out cooling, obtain micro-fluidic chip.
2. the preparation method of papery micro-fluidic chip according to claim 1, it is characterized in that: the first step, pattern has three identical raceway grooves, and the width of every bar raceway groove, length and surveyed area diameter are respectively 200 μm, 9mm and 1.5mm, and the live width of pattern is 1mm.
3. the preparation method of papery micro-fluidic chip according to claim 1, is characterized in that: second step, and dimethyl silicone polymer is that the monomer of 10:1 and initator mix by mass ratio.
4. the preparation method of papery micro-fluidic chip according to claim 1, it is characterized in that: second step, the mass ratio of toluene and dimethyl silicone polymer is 8:1.
5. the preparation method of papery micro-fluidic chip according to claim 1, it is characterized in that: the 3rd step, porous membrane is nitrocellulose membrane.
6. the preparation method of papery micro-fluidic chip according to claim 1, is characterized in that: the 3rd step, and after having printed, the dry 2h of porous membrane, temperature is 80 DEG C.
7. the preparation method of papery micro-fluidic chip according to claim 1, it is characterized in that: the 3rd step, the nozzle temperature of printer and substrate temperature are 25 DEG C, print voltage 30-40V, dot spacing scope 10-80 μm, print number of plies 10-15 layer.
8. the preparation method of papery micro-fluidic chip according to claim 7, is characterized in that: the dot spacing of printer is 30 μm, prints the number of plies 10 layers.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104677896A (en) * | 2015-01-31 | 2015-06-03 | 太原理工大学 | Preparation and application of paper-based microfluidic chip for colorimetric analysis |
| CN105013545A (en) * | 2015-06-30 | 2015-11-04 | 苏州东辰林达检测技术有限公司 | Preparation method of paper-based micro fluidic chip |
| CN105903501A (en) * | 2016-04-18 | 2016-08-31 | 南京大学 | Polydimethylsiloxane-paper composite chip and preparation method and application thereof |
| CN106040326A (en) * | 2016-05-27 | 2016-10-26 | 南京大学 | Macro-porous paper based-polydimethylsiloxane composite microfluidic chip and preparation method and application thereof |
| CN107226262A (en) * | 2017-05-23 | 2017-10-03 | 北京化工大学 | Integrate the papery food drug packing box of paper substrate micro-fluidic chip |
| CN107754961A (en) * | 2017-11-01 | 2018-03-06 | 南通大学 | The preparation method of papery microwell plate based on laser printing |
| CN109116029A (en) * | 2018-07-21 | 2019-01-01 | 福建医科大学 | A method of paper substrate micro-fluidic chip is made based on inkjet printing technology controllability and is detected for glucose quantitation |
| CN109837214A (en) * | 2017-11-28 | 2019-06-04 | 中国科学院大连化学物理研究所 | Three layers of folder film micro-fluidic chip and its preparation based on nitrocellulose basilar memebrane |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104677896A (en) * | 2015-01-31 | 2015-06-03 | 太原理工大学 | Preparation and application of paper-based microfluidic chip for colorimetric analysis |
| CN105013545A (en) * | 2015-06-30 | 2015-11-04 | 苏州东辰林达检测技术有限公司 | Preparation method of paper-based micro fluidic chip |
| CN105903501A (en) * | 2016-04-18 | 2016-08-31 | 南京大学 | Polydimethylsiloxane-paper composite chip and preparation method and application thereof |
| CN106040326A (en) * | 2016-05-27 | 2016-10-26 | 南京大学 | Macro-porous paper based-polydimethylsiloxane composite microfluidic chip and preparation method and application thereof |
| CN106040326B (en) * | 2016-05-27 | 2018-08-17 | 南京大学 | A kind of macropore paper substrate-compound micro-fluidic chip of dimethyl silicone polymer and its preparation method and purposes |
| CN107226262A (en) * | 2017-05-23 | 2017-10-03 | 北京化工大学 | Integrate the papery food drug packing box of paper substrate micro-fluidic chip |
| CN107754961A (en) * | 2017-11-01 | 2018-03-06 | 南通大学 | The preparation method of papery microwell plate based on laser printing |
| CN109837214A (en) * | 2017-11-28 | 2019-06-04 | 中国科学院大连化学物理研究所 | Three layers of folder film micro-fluidic chip and its preparation based on nitrocellulose basilar memebrane |
| CN109116029A (en) * | 2018-07-21 | 2019-01-01 | 福建医科大学 | A method of paper substrate micro-fluidic chip is made based on inkjet printing technology controllability and is detected for glucose quantitation |
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Application publication date: 20141217 |