CN105903501A - Polydimethylsiloxane-paper composite chip and preparation method and application thereof - Google Patents
Polydimethylsiloxane-paper composite chip and preparation method and application thereof Download PDFInfo
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- CN105903501A CN105903501A CN201610239287.2A CN201610239287A CN105903501A CN 105903501 A CN105903501 A CN 105903501A CN 201610239287 A CN201610239287 A CN 201610239287A CN 105903501 A CN105903501 A CN 105903501A
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- polydimethylsiloxane
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- 238000002360 preparation method Methods 0.000 title claims description 15
- 239000002131 composite material Substances 0.000 title abstract description 8
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract description 88
- 239000004205 dimethyl polysiloxane Substances 0.000 claims abstract description 87
- 238000000034 method Methods 0.000 claims abstract description 40
- -1 polydimethylsiloxane Polymers 0.000 claims abstract description 32
- 230000008569 process Effects 0.000 claims abstract description 15
- 230000035515 penetration Effects 0.000 claims abstract description 10
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims description 85
- 150000001875 compounds Chemical class 0.000 claims description 30
- 238000007711 solidification Methods 0.000 claims description 20
- 230000008023 solidification Effects 0.000 claims description 20
- 238000007639 printing Methods 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 15
- 239000011259 mixed solution Substances 0.000 claims description 13
- 239000003795 chemical substances by application Substances 0.000 claims description 12
- 229920000642 polymer Polymers 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 9
- 238000000059 patterning Methods 0.000 claims description 8
- 230000006798 recombination Effects 0.000 claims description 8
- 238000005215 recombination Methods 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 4
- 241000446313 Lamella Species 0.000 claims description 2
- 229920001131 Pulp (paper) Polymers 0.000 claims description 2
- 239000011152 fibreglass Substances 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims description 2
- 239000004745 nonwoven fabric Substances 0.000 claims description 2
- 239000010902 straw Substances 0.000 claims description 2
- 238000009941 weaving Methods 0.000 claims description 2
- 150000001335 aliphatic alkanes Chemical class 0.000 claims 1
- 239000011148 porous material Substances 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 10
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 7
- 238000010276 construction Methods 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 description 15
- 239000000243 solution Substances 0.000 description 11
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 10
- 239000004926 polymethyl methacrylate Substances 0.000 description 10
- 239000012530 fluid Substances 0.000 description 8
- 210000004369 blood Anatomy 0.000 description 7
- 239000008280 blood Substances 0.000 description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 210000003743 erythrocyte Anatomy 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 238000007306 functionalization reaction Methods 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 230000008595 infiltration Effects 0.000 description 3
- 238000001764 infiltration Methods 0.000 description 3
- 238000006116 polymerization reaction Methods 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- BAPJBEWLBFYGME-UHFFFAOYSA-N Methyl acrylate Chemical compound COC(=O)C=C BAPJBEWLBFYGME-UHFFFAOYSA-N 0.000 description 2
- 239000013592 cell lysate Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000010408 film Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 244000086443 Craterellus fallax Species 0.000 description 1
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000008233 hard water Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000011229 interlayer Substances 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 238000010147 laser engraving Methods 0.000 description 1
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000007712 rapid solidification Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007650 screen-printing Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- STZCRXQWRGQSJD-UHFFFAOYSA-M sodium;4-[[4-(dimethylamino)phenyl]diazenyl]benzenesulfonate Chemical compound [Na+].C1=CC(N(C)C)=CC=C1N=NC1=CC=C(S([O-])(=O)=O)C=C1 STZCRXQWRGQSJD-UHFFFAOYSA-M 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000003447 supported reagent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inks, Pencil-Leads, Or Crayons (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
A polydimethylsiloxane-paper composite chip is the polydimethylsiloxane-paper composite chip with controllable depth of penetration that is formed by patterned loading polydimethylsiloxane on paper. A paper chip is easy to process and easy to load a reagent; and a polydimethylsiloxane (PDMS) chip has advantages of mutual fitting, excellent biocompatibility and easy microfluidic pipeline construction on surface. The chip provided by the invention has all advantages of the paper chip and the polydimethylsiloxane chip.
Description
Technical field
The present invention relates to compound chip, particularly polydimethylsiloxane (PDMS) compartmentalization is carried on paper
Polydimethylsiloxane-paper compound chip that upper formation length of penetration is controlled and preparation method thereof.
Background technology
The concept of paper chip is mainly derived from the papery reagent paper of viable commercial product, utilize the porous character of paper allow by
Multiple detectable is carried on the zones of different of paper, and usual papery reagent paper can pass through the direct-vision methodes such as metachromasia,
Directly the many kinds of parameters in sample is characterized, there is cheap, easy-to-use feature.Utilize paper fiber pair simultaneously
The infiltration of liquid and capillarity can guide sample to carry out certain motion in paper chip, thus reach having
The effects such as the pre-separation of tinctorial pattern product, eliminate the dependence to complex fluid driving means, be suitable for amateur state,
As used when professional equipment, layman, rapid broad spectrum screening.Nearly ten years, researcher will
The Conceptual Extension of reagent paper becomes paper chip to be an important development, and the concept of paper chip not only combines the survey of reagent paper
The advantage of examination, also the pipeline in micro-fluidic, fluid controls, process control etc., concept is integrated comes, application model
Enclose and expand further it is considered to be most possibly obtain the microfluidic system of commercialization.Various relevant with paper chip
Technology of preparing, such as cerul patterning techniques, laser-engraving technique, printing technique, the development of paper folding technology etc. is more
It is the possibility enriching paper chip design, has significantly promoted the development in this field.But stationery is born due to it
Having loose structure, the whole scraps of paper are the connection main bodys of a fluid permeable, it is therefore necessary to by certain method
The flowing limiting liquid could realize the restriction to scraps of paper upper fluid flow range, thus the inspection of beneficially quantification
Survey.Hydrophobic material is mainly loaded on the scraps of paper by various patterning methods thus realizes by existing method
Fluid flow scope and the control of order.Such as in ink base paper printing technique and cerul patterning techniques etc..This
The chip structure that class method is produced is the most coarse, even if realizing three-dimensional tube structure by paper folding technology, by
Being all to be made up of the loose structure of paper substrate in pipeline, sample flow speed is slow, and sample adsorption loss is serious, limits
This kind of chip broader practice.
In order to solve this problem, there is seminar to propose chip compound with paper for paper hollow out pipeline, so will
The advantage of fluid line combines with paper chip, has expanded the function and application scope of paper chip.But it is such
Work limit to the most very much, mainly wax and marking ink be required for special handling could realize interlayer be combined.It is true that
Polydimethylsiloxane (PDMS) is used to have multiple advantage as chip material, as being most widely used
Micro-fluidic, PDMS has good printing opacity, breathability, excellent biocompatibility, price
Cheap, processing simplicity, its surface nature particularly hydrophilic and hydrophobic can pass through simple plasma or ozone
Producing the change from hydrophobic to super hydrophilic Deng process, the beneficially control of fluid flow, at this kind of simplicity
Reason can also realize the structure of three dimensional structure chip, makes chip functions more horn of plenty.But compare paper chip,
PDMS thin slice has bigger elasticity, easy deformation, it is difficult to maintain rock-steady structure, is difficult to load modification point simultaneously
Son or other various reagent, limit the application of PDMS.If able to by the advantage of paper chip and PDMS
Advantage combine and learn from other's strong points to offset one's weaknesses, will quickly analyze the fields such as detection for micro-fluidic chip and bring impressive progress.
The existing method being combined with paper by PDMS is mainly and paper surface carries out functionalization group grafting process, so
After utilize these functionalization groups to be chemically crosslinked with PDMS by paper, thus fit together.This kind of side
The cross-linking procedure that method relates to is complicated, it is thus achieved that paper chip the most there is not the relation interpenetrated, paste
Fastness is poor.And process owing to there is the chemical graft to paper, seriously limit the material kind that can load on paper
Class, is unfavorable for the functionalization of paper chip；Can permeate continuously owing to paper relative liquid in this kind of structure is one
Object, therefore in microchip, entirety can only serve as an interface, can application surface narrow.In order to solve this
Problem needs a kind of brand-new PDMS-paper associated methods of exploitation badly can overcome above defect.
Summary of the invention
The subject matter that the invention solves the problems that is how to utilize polydimethylsiloxane (PDMS) and stationery substrate
Combine, be allowed to both retain paper chip and be easily achieved the load of polytype material, the spy of stronger mechanical stability
Point, having again PDMS is prone to the advantage at surface construction microtube structure simultaneously, thus expands paper chip
Range of application.The most just it is an up PDMS and planar patterned precision that paper combines and control
PDMS longitudinal direction in paper cohesive process is combined the degree of depth.
For solving above-mentioned technical problem, the present invention uses PDMS and paper to form interpenetrative composite construction.
Utilize temperature that the regulating and controlling effect of PDMS solidification process speed is controlled scope that PDMS extends on paper and
Penetrate into the degree of depth in paper, be consequently formed PDMS-paper composite construction.Particularly under conditions of temperature is higher
PDMS can realize fast curing-formed within a short period of time, achieves PDMS first more high-precision in substrate
The pattern structure structure that degree, length of penetration are controlled.
Technical scheme is as follows:
A kind of polydimethylsiloxane-paper compound chip, it is a kind of by polydimethylsiloxane (PDMS) figure
Case is carried on paper and forms polydimethylsiloxane-paper compound chip that length of penetration is controlled.
Above-mentioned compound chip, described paper refers to all kinds of material with loose structure, such as wood pulp or straw pulp system
Become stationery, by weaving porous sheet, Non-woven fabrics lamella, polymer-based or fiberglass-based perforated membrane.
A kind of method preparing above-mentioned compound chip, it comprises the steps:
Step 1. prepares polydimethylsiloxane (PDMS) performed polymer and the mixed solution of firming agent；
Step 2, by writing, seal, printing or method for printing screen, by above-mentioned polydimethylsiloxane
Mixed solution transfers to the specific region of paper substrate；
Step 3. waits 0 second 30 minutes；
Whole material is made polydimethylsiloxane solidification prepare poly-diformazan 100 DEG C of-200 DEG C of heating by step 4.
Radical siloxane-paper compound chip.
The preparation method of above-mentioned compound chip, described printing process can be the method printed with plane or volume to volume
Printing.
The preparation method of above-mentioned compound chip, the polydimethylsiloxane paper recombination process narrated can be once to gather
Dimethyl siloxane paper recombination process, or repeatedly polydimethylsiloxane paper recombination process.
The application in separating, analyze, differentiate or identifying of the above-mentioned compound chip.
The concrete effect of the present invention is as follows:
The present invention utilizes the feature that polydimethylsiloxane controllable time at relatively high temperatures is polymerized, in conjunction with paper substrate figure
The method of case, the waiting time before being solidified by control and solidification temperature, it is achieved that patterning, controlled ooze
The PDMS-paper complex method of the degree of depth thoroughly.Utilize this novel polydimethylsiloxaneoxygen-enrichment-paper compound chip preparation side
Method, it is possible to make the chip of gained both have paper chip and be prone to processing, be prone to the advantage of supported reagent, have again poly-
Dimethyl siloxane (PDMS) chip can fit mutually, has the biocompatibility of excellence and be prone at it
The advantage that surface carries out Micro-flow pipe structure.
Accompanying drawing explanation
Fig. 1. polydimethylsiloxane (PDMS) front aggressiveness and curing agent mixture vary with temperature hardening time
Relation.The front aggressiveness herein selected and firming agent ratio are 10:1.When temperature rises to more than 100 degrees Celsius,
Hardening time drops to reduced levels；Along with solidification temperature raises, curing rate is accelerated.
Fig. 2. polydimethylsiloxane (PDMS) paper chip patterning condition analysis.A.PDMS infiltration is deep
Degree and solidification temperature relation.Show that raising solidification temperature can reduce the PDMS length of penetration to paper substrate.B. filter
Paper PDMS planar diffusion scope and solidification temperature relation；C. printing paper PDMS planar diffusion scope and solidification
Temperature relation, shows that higher temperature is conducive to suppressing the planar diffusion of PDMS, thus obtains preferably figure
Case resolution.
The paper chip that Fig. 3 .A1-A2: polydimethylsiloxane (PDMS) patterns；B. PDMS is combined
The novel PDMS-paper chip sample of pipe layers.The PDMS paper chip combining PDMS pipe layers is the most sharp
The preserved pipeline formed between paper using and PDMS makes fluid flowing rather than traditional paper chip utilize paper
Capillarity transmit liquid, so make the flowing of liquid more quick, decrease liquid flowing simultaneously
During loss.
Fig. 4. for polymethyl methacrylate (PMMA) seal of chip desirable pattern.Wherein canescence
Part is PMMA seal region.Utilize this seal can make the patterning chip being similar in Fig. 3.
Fig. 5 .A: the PDMS paper chip printed by inkjet technology.This chip can utilize swan-neck
Road is used for premixing and the response analysis of liquid.B: can be used for preparing the PMMA mould of chip upper strata pipeline.
During use, PMMA solution is poured on mould and is heating and curing.
Fig. 6 .A: the PDMS paper chip printed by screen printing technique.This chip can utilize swan-neck
Road is used for premixing and the response analysis of liquid.B: can be used for preparing the PMMA mould of chip upper strata pipeline.
During use, PMMA solution is poured on mould and is heating and curing.
Fig. 7. for the poly-methyl of preparation process of the polydimethylsiloxane-paper compound chip of multistep recombination process
Acrylic acid methyl ester. (PMMA) seal.A. the seal that the length of penetration in front is deeper it is used for；B. for the back side
The seal that length of penetration is shallower.The white space alignment both reserved is needed during use.
Detailed description of the invention:
The invention will be further described with example below in conjunction with the accompanying drawings.
Embodiment 1: the preparation of polydimethylsiloxane-paper compound chip
Step 1. prepares polydimethylsiloxane (PDMS) performed polymer and the mixed solution of firming agent, performed polymer
It is 10:1 with firming agent mass ratio；
Step 2. carves the seal of chip desirable pattern, and Fig. 4 is shown in by its pattern, will be carved with chip desirable pattern
Seal picks PDMS, then cover printing paper (Zhenjiang Dadong Paper Co., Ltd., in emerging extra white xerography
Paper) surface；
2 seconds step 3. waiting time；
Seal 200 DEG C of solidifications, is taken off after solidification by step 4. by whole material, prepares polydimethylsiloxanes
Alkane-paper compound chip.
PDMS mixed solution is poured on the seal shown in Fig. 4 by step 5. so that pattern is completely submerged；
Step 6.PDMS heats 30min solidification at 60 DEG C, peels off seal with elbow tweezer；
After the PDMS reverse mould pipe surface obtained is dipped a small amount of PDMS solution by step 7., with refill before
Sheet pipe alignment is fitted；
Whole material is solidified 2s at 200 DEG C by step 8., prepares NEW TYPE OF COMPOSITE chip after solidification；
Step 9. drip respectively in each border circular areas of the periphery of chip methyl orange solution, colourless phenolphthalein solution,
C.I. 14645. solution, starch solution, luminol reagent；
Step 10. entreat in the chips pipeline handover region successively inject dilute hydrochloric acid solution, diluted sodium hydroxide solution,
Hard water, iodine water, blood sample also press the border circular areas at corresponding indicator place also the most respectively
Unclamp, make two kinds of solution be fully contacted reaction respectively.
Embodiment 2: the preparation of polydimethylsiloxane-paper compound chip
Step 1. prepares polydimethylsiloxane (PDMS) performed polymer and the mixed solution of firming agent, pre-polymerization
Body and firming agent mass ratio are 10:1；
Step 2. passes through printing of inkjet printer, and by chip desirable pattern, this mixed solution is printed to printing paper
On, see Fig. 5 A；
Step 3. waits 30 minutes；
Whole material 100 DEG C of solidifications, is prepared polydimethylsiloxane-paper compound chip by step 4..
PDMS mixed solution is poured on the seal shown in Fig. 5 B by step 5. so that pattern is soaked completely
Not yet；
Step 6.PDMS heats 30min solidification at 60 DEG C, peels off seal with elbow tweezer；
After the PDMS reverse mould pipe surface obtained is dipped a small amount of PDMS solution by step 7., with refill before
Sheet pipe alignment is fitted；
Whole material is solidified 2min at 100 DEG C by step 8., prepares NEW TYPE OF COMPOSITE chip after solidification；
Step 9. is by human blood sample and erythrocyte cracked liquid (triumphant base is biological, lot number KGP1150-KGP11100)
It is injected separately into the sample delivery point above y-branch；
Step 10. is pressed the PDMS film covered on the liquid storage tank of right side and is unclamped, allows blood sample and cell
Lysate fully vibrates after crossing at y-branch mixing in pipeline；
The mixed sample of step 11. stands 10min after arriving liquid storage tank makes erythrocyte crack completely.
Obtain has cracked erythrocytic blood sample and sucking-off can carry out subsequent analysis again.Due to the sample used
Measure less, so being suitable for mixing and the subsequent treatment of micro-example.
Embodiment 3: the preparation of polydimethylsiloxane-paper compound chip
Step 1. prepares polydimethylsiloxane (PDMS) performed polymer and the mixed solution of firming agent, pre-polymerization
Body and firming agent mass ratio are 10:1；
Step 2. is printed by quick silk screen, transfers on printing paper by this mixed solution by chip desirable pattern,
See Fig. 6 A；
Step 3. waits 0 second；
Paper 150 DEG C of rapid solidifications, is prepared polydimethylsiloxane-paper compound chip by step 4..
PDMS mixed solution is poured on the seal shown in Fig. 6 B by step 5. so that pattern is soaked completely
Not yet；
Step 6.PDMS heats 30min solidification at 60 DEG C, peels off seal with elbow tweezer；
After the PDMS reverse mould pipe surface obtained is dipped a small amount of PDMS solution by step 7., with refill before
Sheet pipe alignment is fitted；
Whole material is solidified 2min at 100 DEG C by step 8., prepares NEW TYPE OF COMPOSITE chip after solidification；
Step 9. is by human blood sample and erythrocyte cracked liquid (triumphant base is biological, lot number KGP1150-KGP11100)
It is injected separately into the sample delivery point above y-branch；
Step 10. is pressed the PDMS film covered on the liquid storage tank of right side and is unclamped, allows blood sample and cell
Lysate fully vibrates after crossing at y-branch mixing in pipeline；
The mixed sample of step 11. stands 10min after arriving liquid storage tank makes erythrocyte crack completely.
Obtain has cracked erythrocytic blood sample and sucking-off can carry out subsequent analysis again.Due to the sample used
Amount less, so being suitable for the mixing of micro-example and subsequent treatment.
Embodiment 4: the preparation of the polydimethylsiloxane of multistep recombination process-paper compound chip
Step 1. prepares polydimethylsiloxane (PDMS) performed polymer and the mixed solution of firming agent, pre-polymerization
Body and firming agent mass ratio are 10:1；
Needed for step 2. will be carved with chip, the seal of the deeper pattern of polydimethylsiloxane length of penetration picks
PDMS, covers and specifies region on filter paper (Hangzhou Wo Hua filter paper company limited enjoy a double blessing board 9mm qualitative filter paper),
Such as Fig. 7 A；
Step 3. waits 10 minutes, makes PDMS thoroughly infiltrate through paper substrate；
Step 4. by whole material 100 DEG C of solidifications；
Needed for step 5. will be carved with chip, the seal of the shallower pattern of polydimethylsiloxane length of penetration picks
PDMS, covers appointment region on filter paper, such as Fig. 7 B；
Step 6. waits 0 second；
Whole material 180 DEG C of solidifications, is prepared polydimethylsiloxane-paper compound chip by step 7..
The preparation of step 8. yeast agar culture medium: weigh sodium nitrate 3.0g, disodium hydrogen phosphate 1.0g, chlorine
Change potassium 0.5g, bitter salt 0.5g, ferrous sulfate 0.01g, sucrose 30.0g, agar 30g, distilled water
1000ml dissolves, 121 DEG C of high temperature sterilizes.
The preparation of step 9. yeast culture fluid: repeat the operation of step 8, removes the step adding agar；
Step 10. is when culture medium solution is reduced to about 40 DEG C, by yeast suspension vol than 1:1 and training
Support base mixing；
Mixed liquor is dropped on polymethyl methacrylate (PMMA) thin film by step 11., suppresses agar thin slice；
Thin slice is lain in dot matrix area, side, chip shallower seal face by step 12., and chip pipe line area is placed in cultivation
In liquid, observed a Yeast Growth situation every 2 hours.It appeared that yeast can realize on chip
Persistently divide growth.
The patterning infiltration the method achieving multiple different depth PDMS structure on a paper substrate controls.
Claims (6)
1. polydimethylsiloxane-paper compound chip, is characterized in that: it is a kind of by polydimethylsiloxanes
Alkane patterning is carried on paper and forms polydimethylsiloxane-paper compound chip that length of penetration is controlled.
Compound chip the most according to claim 1, is characterized in that: described paper refer to all kinds of have many
The material of pore structure, the stationery made such as wood pulp or straw pulp, by weaving porous sheet, Non-woven fabrics lamella,
Polymer-based or fiberglass-based perforated membrane.
3. the method preparing compound chip described in claim 1, is characterized in that it comprises the steps:
Step 1. prepares polydimethylsiloxane (PDMS) performed polymer and the mixed solution of firming agent；
Step 2, by writing, seal, printing or method for printing screen, by above-mentioned poly dimethyl silicon
Oxygen alkane mixed solution transfers to the specific region of paper substrate；
Step 3. waits 0 second 30 minutes；
Whole material is made polydimethylsiloxane solidification prepare poly-two 100 DEG C of-200 DEG C of heating by step 4.
Methylsiloxane-paper compound chip.
4., according to the preparation method of the above-mentioned compound chip of claim 3, it is characterized in that: described printing process
It it is the method printing printed with plane or volume to volume.
The preparation method of compound chip the most according to claim 3, is characterized in that: described poly dimethyl silicon
Oxygen alkane paper recombination process is a polydimethylsiloxane paper recombination process, or repeatedly polydimethylsiloxane
Paper recombination process.
6. the application in separating, analyze, differentiate or identifying of the compound chip described in claim 1.
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| CN103203257A (en) * | 2013-03-12 | 2013-07-17 | 西安交通大学 | Method for manufacturing paper micro-fluidic chip |
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| CN109482247B (en) * | 2018-10-18 | 2021-04-13 | 哈尔滨工业大学（深圳） | Micro-fluidic chip manufacturing process and micro-fluidic chip |
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