CN105642377B - No benzene facture of microchip method based on 3 D-printing and product - Google Patents
No benzene facture of microchip method based on 3 D-printing and product Download PDFInfo
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- CN105642377B CN105642377B CN201610061308.6A CN201610061308A CN105642377B CN 105642377 B CN105642377 B CN 105642377B CN 201610061308 A CN201610061308 A CN 201610061308A CN 105642377 B CN105642377 B CN 105642377B
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The no benzene facture of microchip method based on 3 D-printing that the invention discloses a kind of, including：(1) threedimensional model of micro-fluidic chip substrate of the design with flow path groove；(2) three-dimensional printer print software is utilized, the micro-fluidic chip substrate with flow path groove is printed；(3) printed micro-fluidic chip substrate had into the mixture that the one side of flow path groove smears a strata dimethyl siloxane and curing agent, drying；(4) slurry is poured into flow path groove, dries, obtains the no benzene micro-fluidic chip based on 3 D-printing.The present invention is based on the production methods of the micro-fluidic chip of 3D printing, and process is simple and efficient, and production efficiency is high, is easy to industrialization large-scale production.Micro-fluidic chip made from the method for the present invention can realize that unlimited circulating repetition utilizes, and greatly reduce cost.
Description
Technical field
The present invention relates to micro fluidic chip technical fields, more particularly, to one kind based on 3D printing technique manufacture without drive speed
The production method of programmable micro-fluidic chip.
Background technology
Micro-fluidic chip is otherwise known as chip lab, is a kind of technology manipulated to fluid on micro-meter scale.
The technology will be on the basic function micro of chemistry and biology laboratory to one the only chip of several square centimeters of sizes.It is logical
The intersection of the subjects such as analytical chemistry, micro electro mechanical processing, computer, electronics, material system and biology, medicine is crossed, is realized from sample
Product are handled to the micromation of detection, automation, integrated and portability.Micro-fluidic chip has a variety of monotechnics flexible combinations
And the characteristics of large-scale integrated, just great information content can be obtained with a small amount of sample in this way, more likely surmount single analysis
Function becomes the miniature diversity operation platform of an entirety.Currently, micro-fluidic chip because its micromation, automation, it is integrated and
The great potential of portable flower aspect, is gradually applied in environmental monitoring and the research and development of pollutant analysis technology, and obtain
A series of impressive progresses.
Application No. is the patent document of CN201310341700.2 disclose it is a kind of for micro-fluidic chip simplicity quickly
Hot-press method, will need carry out thermocompression bonding or hot-forming thermoplasticity micro-fluidic chip alignment after, be placed on one
On the stainless steel plate of surface polishing；Micro-fluidic chip is fixed on stainless steel using the magnetic material of one or more specific shapes
In the lower plate of hot press of material；The stainless steel plate for being fixed with micro-fluidic chip is attached among two heating platens of hot-press equipment,
After applying specific temperature and pressure, hot pressing is completed.
Application No. is the patent documents of CN201310341536.5 to disclose a kind of laser processing of micro-fluidic chip,
The high molecular polymer chip of generation processing is cleaned up, is dried up by it；By the macromolecule membrane coating of 1~30 micron thickness
In work surface；Using carbon dioxide laser equipment, it is micro- to go out through-hole, channel, mixer in polymer core on piece Carving Machining
Etc. structures；Macromolecule membrane is removed from chip surface, obtains the micro-fluidic chip for being machined with micro-structure.
The manufacturing method of existing micro-fluidic chip mainly produces one side by the methods of photoetching, hot pressing and carries micro-nano
The chip of rice igfet channel, itself and cover plate then be bonded to obtain.Traditional micro-fluidic chip generally use glass, organic glass
The materials such as glass, organosilicon, the pump driving reagent that cost of manufacture is relatively high and needs are accurate flows in runner to be reacted.
Invention content
The present invention provides a kind of repeatable micro-fluidic chips utilized of no benzene based on FDM three-dimensional printing technologies
Production method, micro-fluidic chip can be manufactured easily and fast.
Present invention simultaneously provides one kind by the prepared micro-fluidic chip of the above method and micro-fluidic chip component, this is micro-
Fluidic chip and micro-fluidic chip component package and opening are convenient, highly practical.
A kind of no benzene facture of microchip method based on 3 D-printing, includes the following steps：
Step (1)：Micro-fluidic chip substrate threedimensional model of the design with flow path groove；
Step (2)：Threedimensional model is generated into STL formats, imports FDM three-dimensional printer print softwares, sets printing ginseng
Number.The micro-fluidic chip substrate of flow path groove is provided using file printings such as polylactic acid (PLA)；
Step (3)：There is the one side of flow path groove to smear a strata dimethyl siloxane material printed PLA substrates
(PDMS), the substrate for smearing PDMS is then put into 50~70 degrees Celsius of drying boxes to dry 40~120 minutes.
Step (4)：One layer is laid in the flow path groove inner surface of micro-fluidic chip substrate to make sample to be tested with capillary
Driving layer firmly obtains the no benzene micro-fluidic chip based on 3 D-printing.
Driving layer material can select according to actual needs, need to ensure that driving layer makees detected sample with capillary
Firmly, such as the stronger sample of polarity, hydrophilic driving layer material may be selected；It, can be with for the weaker sample of polarity
Select the stronger driving layer material of lipophile.
There are two ways to step (4) is laid with driving layer：
The first is paving slurry processes：Cellulose powder and water are carried out in certain proportion to be mixed into slurry, it then will be fine
The plain slurry material of dimension pours into flow path groove, is then placed in 50~70 degrees Celsius of drying boxes 40~120 minutes.The cellulose powder
It is either one or more in starch, chitin, hemicellulose or replace with cellulose and starch, chitin to can be replaced paper pulp
One or more mixture in matter, hemicellulose；The paper pulp may be selected common wood pulp, straw pulp, jute pulp, reed pulp, sugarcane slurry,
Bamboo pulp, rag stock etc..
Second is direct place mat method：The place mat flexibility paper in flow path groove obtains driving without pump based on 3 D-printing
Dynamic micro-fluidic chip.
In step (1), according to design requirement, existing mature technology can be used in structure micro-fluidic chip substrate model, can lead to
It crosses computer aided design cad (Computer Aided Design) software and obtains three-dimensional model diagram, can be used business CAD soft
Part, such as CorelDraw, the designs such as Solidworks.
To improve printing precision, the filling rate of FDM printings can be properly increased, preferably, filling rate is in print procedure
50~80；Further preferred filling rate is 60, and since chip base size is that Centimeter Level is other, the time-write interval is also relatively
It is short.The depth of flow path groove can be arranged to 0.2mm-1mm according to demand.
In step (3), PDMS is the mixture of dimethyl silicone polymer and curing agent.The curing agent be and existing skill
The mass ratio of the curing agent mating with dimethyl silicone polymer in art, dimethyl silicone polymer and curing agent is generally 8-15:1.
It can effectively be obtained in water passage surface formation protective filmy layer by uniformly applying one layer of PDMS in flow path groove one side, prevent reagent
Penetrate into PLA chip bases.The present invention is modified runner groove surfaces using the PDMS configured in specific proportions simultaneously,
Enable runner that there is preferable hydrophobicity and surface quality, to be utilized to achieve the purpose that repeat.
In step (4), cellulose powder and deionized water are with mass ratio 1:The proportioning of 3-8 is mixed, and is answered when pouring into runner
The slurry in runner is set to reach saturation state using the principle of surface tension of liquid.It is put down after capable of just being dried in this state
Whole runner effect.When cellulose powder replaces with other powders, the mass ratio of powder and water can be adjusted as needed, to meet
Actual needs.
Complete just to complete after above four steps the making of entire micro-fluidic chip, micro-fluidic core made from this method
Piece can wash with water the cellulose powder (or other powders) in runner after using, and repeat step (4)
Realize the recycling of micro-fluidic chip.
The present invention also provides a kind of micro-fluidic chip, by described in any of the above-described technical solution based on 3 D-printing
No benzene facture of microchip method makes to obtain.
The micro-fluidic chip of various structures can be made using the preparation method of the present invention.
Preferably, the micro-fluidic chip includes substrate, the substrate side is equipped with：
Center adding mouth at the center of substrate；
Several side adding mouths circumferentially disposed along the center of substrate；
The runner that side adding mouth is connected to center adding mouth respectively.
The present invention also provides a kind of micro-fluidic chip components, including micro-fluidic chip and the cover are on micro-fluidic chip
Cover plate；
If the micro-fluidic chip is equipped with center adding mouth, several side adding mouths around center adding mouth and general
The runner that dry side adding mouth is connected with center adding mouth respectively；
The cover plate is equipped with through-hole corresponding with the center adding mouth and side adding mouth；
The micro-fluidic chip is rotatably assorted with the cover, and the cover plate, which has, ensures all adding mouths on micro-fluidic chip
The sample-adding working position of (including center adding mouth and side adding mouth) conducting corresponding with the through-hole, and block all sides and add
The encapsulation work position of sample mouth；
Plectrum is equipped between the micro-fluidic chip and cover plate, the plectrum when cover plate is in encapsulation work position for covering
The center adding mouth.
When using above-mentioned micro-fluidic chip component, it is only necessary to which rotating cover plate can facilitate realization cover plate to work at two
Switching, realizes the encapsulation to micro-fluidic chip and sample-adding respectively；When cover plate is in sample-adding working position, on micro-fluidic chip in
Heart adding mouth and side adding mouth are simultaneously with corresponding through-hole to being just connected, at this time, it can be achieved that sample-adding to micro-fluidic chip；Add
After the completion of sample, or when not in use, cover plate can be rotated to encapsulation work position, cover plate blocks side adding mouth at this time, simultaneously
Plectrum blocks center adding mouth, completes the encapsulation to micro-fluidic chip, avoids the influence of outer bound pair micro-fluidic chip.
Preferably, described plectrum one end connects with cover plate axis, which is equipped with pilot hole, pilot hole and pivoted connection end
Between be equipped with covering center adding mouth occlusion part；The micro-fluidic chip is equipped with the connecting rod across the pilot hole, works as lid
When piece is rotated relative to micro-fluidic chip, the final covering realized to center adding mouth of plectrum rotation and opening are driven.
Preferably, the center adding mouth is located at the center of micro-fluidic chip, the side adding mouth is along micro-fluidic core
Piece is circumferentially uniformly distributed.
Preferably, being equipped with to level between the micro-fluidic chip and cover plate.Facilitate cover plate and micro-fluidic chip it
Between alignment.
Preferably, the depth of the runner is 0.2mm-1mm.
Preferably, the micro-fluidic chip is equipped with center adding mouth and a side surface of side adding mouth is covered with poly- two
Methylsiloxane cured film；Dimethyl silicone polymer solidification film surface is covered with cellulose powder.
The present invention makes substrate using FDM three-dimensional printing technologies, by the way of powdering, micro-fluidic chip is made.Make
To be preferred, used powder is cellulose powder, for white odorless, tasteless powder, not soluble in water, acetone, ethyl alcohol or toluene, property
Stablize, is highly suitable as test experience carrier.The diameter of cellulose powder is 74-125 μm, the particle of micron level size
Aggregation has capillarity, detection reagent can be allowed to flow to reaction tank automatically in runner through capillary action, is not necessarily to other
Outer power drive enormously simplifies the condition needed for detection, the present invention can be applied in various clinical detections.This method has can
Recycling, no benzene, flowing velocity is adjustable, runner high resolution, it is at low cost the advantages that.
Compared with prior art, the invention has the advantages that：
The present invention is based on the production methods of the micro-fluidic chip of 3D printing, and process is simple and efficient, and production efficiency is high, easily
In industrialization large-scale production.Micro-fluidic chip substrate obtained theoretically may be implemented to recycle infinitely by the method for the invention
Recycling, greatly reduces cost.Due to this method using cellulose powder as runner medium, through capillary action come
The flowing for realizing reagent facilitates detection without accurate air pump driving device.Secondly, white cellulose powder is as medium,
In colorimetric test, it is convenient to the variation of reagent observation color.In addition, the present invention have in terms of cell culture and flow field control it is original
Performance and convenient for encapsulation.
The micro-fluidic chip component overall structure of the present invention is simple, easy to use, only can be realized by simply rotating
The switching of micro-fluidic chip working condition, encapsulates and opens very convenient, and practicability is stronger.
Description of the drawings
Fig. 1 is the schematic diagram of micro-fluidic chip substrate in the present invention；
Fig. 2 is the schematic diagram for being filled with cellulose powder slurry in the present invention in micro-fluidic chip substrate flow path groove.
Fig. 3 is final micro-fluidic chip schematic diagram obtained in the present invention.
Fig. 4 is that the gray-scale map that micro-fluidic chip carries out nitrite detection is made using the method for the present invention.
Fig. 5 is gray scale-concentration fitted figure of nitrite detection.
Fig. 6 is the Y shape T-SENSOR lab diagrams that the method for the present invention makes.
Fig. 7 is that the close and distant water flow passage resolution analysis of the method for the present invention micro-fluidic chip is tested.
Fig. 8 is the method for the present invention micro-fluidic chip cell culture experiments figure.
Fig. 9 (a) is the side structural schematic diagram of the micro-fluidic chip component of the present invention.Fig. 9 (b) is the micro-fluidic of the present invention
The other side structural schematic diagram of chip assembly.
Figure 10 is the method for the present invention micro-fluidic chip encapsulation schematic diagram and pictorial diagram.
In figure, 1- micro-fluidic chip substrates, 2- cellulose powder slurries, cellulose powder runner after 3- dryings, 4- detection zones, 5-
Detection reagent injection region, No. bis- liquid dyes channels 6-, 7- No.1 liquid dyes channel, the hydrophilic runner resolution ratio experiments of 8-, 9-
Most thin hydrophilic channel, the hydrophobic dam resolution ratio experiments of 10-, the most thin hydrophobic dams 11-.
Specific implementation mode
Embodiment：By taking the detection of a quincuncial facture of microchip and nitrite as an example, the present invention is carried
And manufacture craft further illustrate.
Fig. 1 shows the threedimensional model of designed quincunx micro-fluidic chip substrate 1, by the threedimensional model file generated
Obtained STL formatted files are imported into 3 D-printing software by STL formatted files, and setting print parameters filling rate is 60.Even
It is connected to computer, and starts FDM three-dimensional printers, is controlled by computer and moves FDM three-dimensional printer work, start micro-fluidic core
The print procedure of piece substrate 1.Existing printed material can be selected, for example polylactic acid (PLA), acrylonitrile-butadiene-benzene can be selected
Ethylene copolymer (ABS) etc., selects polylactic acid in the present embodiment.PLA micro-fluidic chips are removed after the completion of printing from printer
Substrate 1.
With dimethyl silicone polymer and curing agent 8-15:1 mass ratio prepares PDMS, is waited in PDMS after standing 20min
Bubble completely disappear after (in the present embodiment the mass ratio of dimethyl silicone polymer and curing agent be 10:1), by it is uniform
It is coated in the one side that micro-fluidic chip substrate 1 carries runner, forms a thin layer of protective layer.The micro-fluidic chip of PDMS will be coated
Curing process is dried in the drying box that substrate is put into 60 degrees Celsius, and the micro-fluidic core with PDMS protective layers is taken out after 1 hour
Piece substrate.
With cellulose powder and deionized water 1:5 mass ratio configures cellulose powder slurry, after stirring evenly.By cellulose
Slurry material is so uniform that pour into the flow path groove of the micro-fluidic chip substrate with PDMS protective layers, and Fig. 2 shows micro-fluidic cores
Piece substrate pours into the state diagram of cellulose powder slurry 2, using the effect of surface tension of liquid, enables the cellulose powder in flow path groove
Slurry reaches expanded state.It is so steady that be put into 60 degrees Celsius of drying boxes by the micro-fluidic chip substrate with cellulose powder slurry
Drying and processing is carried out, is taken out after 1 hour.Fig. 3 is the schematic diagram that micro-fluidic chip is finally made after drying, fiber after being dried in figure
Plain powder runner 3 has preferable capillary force due to the effect of cellulose powder.
In Fig. 4, nitrate detection agent is instilled in detection reagent injection region 5, waits for that nitrate detection agent is spread completely
To 8 detection zones 4, drop nitrite (a concentration of 10mg/L, 8mg/L, 6mg/L, 4mg/L, a 3mg/ is respectively instilled in detection zone 4
L, 2mg/L, 1mg/L) normal concentration reagent and a drop test agent to be checked.Colour developing staircase chart shown in Fig. 4 is obtained, is passed through
PHOTOSHOP photo handling softwares extract the gray value of each detection zone color, by the concentration and ash of normal concentration reagent
Angle value is as standard, using the cutting edge aligned Trendline of least square fitting.
Fig. 5 is concentration-gray scale linear trend figure of normal concentration reagent, according to the gray scale of linear equation and area to be detected
Value, it is 7.4mg/L that reagent concentration to be detected, which is calculated, and error can receive compared with preset 7mg/L, and according to figure
The R of 5 displays2It can be seen that reliability (the R of Trendline2For the ratio of regression sum of square and total sum of squares of deviations, total deviation is indicated
The ratio that can be explained by regression sum of square in quadratic sum, this ratio are the bigger the better, and model is more accurate, and regression effect is more aobvious
It writes.R2Between 0~1, closer to 1, regression fit effect is better, it is considered that the models fitting goodness more than 0.8 compares
It is high).
Fig. 6 is the micro-fluidic chip that is made using method of the present invention to realize experiment that T-SENSOR makes.It is real
It tests and is divided into two groups；Respectively as shown in (b) in (a) in Fig. 6 and Fig. 6, the entrance of Y types runner is arranged two in micro-fluidic chip, point
Not Wei No.1 liquid dyes channel 6, No. two liquid dyes channels 7.
In wherein Fig. 6 (a) use micro-fluidic chip in Y type runner two entrances length be respectively 15.5mm,
4.5mm, width 2mm, depth 0.5mm.The length phase of Y type runner two entrances in the micro-fluidic chip that (b) is used in Fig. 6
Deng, respectively 15.5mm, 15.5mm, width 2mm, depth 0.5mm；The sprue width of Y types runner is 4mm in Fig. 6.
Two groups of micro-fluidic chips are respectively separated first and fix time, and record liquid distinguishes situation：The time of (a) in wherein Fig. 6
Point is respectively 30s, 60s, 120s；The time point of (b) is respectively 30s, 90s, 120s in Fig. 6.Record in result such as Fig. 6 (a) and
(b) shown in.
It will be appreciated from fig. 6 that in this experiment, No.1 liquid dyes channel 7 and No. two 6 clear-cuts of liquid dyes channel,
After 120min, entire runner is uniformly distributed according to the length of liquid feeding end is inversely proportional：The width of (a) different colours is in Fig. 6
0.9mm (No.1 liquid), 3.1mm (No. two liquid)；The width of (b) different colours is 2mm (No.1 liquid) and 2mm (two in Fig. 6
Number liquid).Fully demonstrate the excellent flow field control ability of micro-fluidic chip made by the method for the invention.
Fig. 7 is the minimum resolution reality carried out in resolution ratio to the micro-fluidic chip made by this experiment the method
It tests, has made a series of close and distant water flow passage of gradients respectively, can be obtained most thin hydrophilic runner 9 (in such as Fig. 7 (a) to detect
It is shown) and most thin hydrophobic dam 11 (in such as Fig. 7 shown in (b)), wherein：(a) is that hydrophilic runner resolution ratio is tested in 8, Fig. 7 in Fig. 7
(b) it is the partial enlarged view that (c) in 10, Fig. 7 of hydrophobic dam resolution ratio experiment is part A in (a) in Fig. 7, (d) is in Fig. 7 in Fig. 7
(b) partial enlarged view of part B in.As can be seen from Figure, the attainable most thin hydrophilic runner 9 of institute and most carefully hydrophobic dam 11 divides
It is not 118 and 493 microns.The requirement of most of micro-fluidic detection application can be met.
Fig. 8 is the experiment that cell culture is carried out using the micro-fluidic chip made by this experiment the method.Pass through miniflow
It controls chip and real-time feed culture solution is carried out to cell, and compared with conventional cell cultural method：Wherein (a) is cell culture
Chip schematic diagram (left side) and cell cultivation chip pictorial diagram (right side), (b) be place 1 day, 4 days, 7 days after cell dyeing after it is microcosmic
Figure；(c) it is cell survival rate comparison diagram.By correction data as can be seen that the micro-fluidic chip made by this method is in cell
The survival rate of peer-level can substantially be reached in terms of culture with traditional cell culture processes.In addition, what the present invention made
Micro-fluidic chip is the channel design of opening, is easy to the controllable deposition cell on chip, is easy to merge with cell printing technique.It fills
Micro-fluidic chip made by clear this method of defending oneself has huge application prospect in terms of cell culture.
Fig. 9 (a) and Fig. 9 (b) show the structural schematic diagram of the micro-fluidic chip component of the present invention：A kind of micro-fluidic chip
Component includes the cover plate 102 of micro-fluidic chip 101 and the cover on micro-fluidic chip, and micro-fluidic chip adds equipped with center
Sample mouth 103, around center adding mouth several side adding mouths 104 and by several side adding mouths respectively with center adding mouth
The runner 105 of conducting.Center adding mouth is located at the center of micro-fluidic chip, and the side adding mouth is circumferentially equal along micro-fluidic chip
Even distribution.
Cover plate 102 is equipped with through-hole corresponding with center adding mouth 103 and side adding mouth 104 106；These through-holes 106
It is distributed at cover plate center and surrounding, it is corresponding with center adding mouth 103 and side adding mouth 104 respectively.Cover plate 102 is semi-surrounding
Bucket body structure, surround accommodate micro-fluidic chip 101 space.The opening position fold inward of cover plate 102, it is micro-fluidic to form blocking
What chip 101 slid blocks side 107.101 both sides of micro-fluidic chip are set there are two hangers 108, are set on the rib 107 of cover plate 102
There is the merging hole 109 coordinated with hangers 108, is used for the merging of micro-fluidic chip 101.
Micro-fluidic chip 101 is rotatably assorted with the cover 102, and cover plate, which has, ensures all adding mouths and institute on micro-fluidic chip
The sample-adding working position that through-hole corresponds to conducting is stated, and blocks the encapsulation work position of all side adding mouths.
Plectrum 110 is equipped between micro-fluidic chip 101 and cover plate 102, which is used to be in encapsulation work position in cover plate
When cover the center adding mouth.110 one end of plectrum is connect by axis 111 and cover plate axis, which is equipped with pilot hole
112, pilot hole is strip-shaped hole, and the occlusion part 113 of covering center adding mouth is equipped between pilot hole and pivoted connection end；Micro-fluidic chip
It is equipped with the connecting rod 114 across the pilot hole, when cover plate is rotated relative to micro-fluidic chip, drives plectrum rotation is final to realize
Covering to center adding mouth and opening.
Figure 10 is the example being packaged to the micro-fluidic chip made by this method the method.(a), (b) point in figure
Closure Wei not encapsulated and open two status diagrams, (c), (d), (e) be the pictorial diagram that is packaged of micro-fluidic chip, (c),
(d) when being opened for chip, the pictorial diagram being loaded is (e) after the completion of being loaded, to close the pictorial diagram of chip.The device passes through
Round plum blossom monitor station is rotated to reach closure and open the switching between two states.It can be light by the structure for designing certain
Be easy to get and micro-fluidic chip be packaged, and can random switching state, need detection when, device is adjusted to open shape
State is detected experiment, when without detecting, device is adjusted to closed state, protects detection device.In micro-fluidic detection mistake
Have very great significance in journey.
Claims (10)
1. a kind of no benzene facture of microchip method based on 3 D-printing, which is characterized in that including：
(1) threedimensional model of micro-fluidic chip substrate of the design with flow path groove；
(2) threedimensional model is imported in three-dimensional printer, using three-dimensional printer print software, prints and be with depth
The micro-fluidic chip substrate of the flow path groove of 0.2mm-1mm；
(3) there is the one side of flow path groove to smear a strata dimethyl siloxane and solidification printed micro-fluidic chip substrate
The mixture of agent, drying form protective layer；
(4) be laid with one layer in the flow path groove inner surface with matcoveredn of micro-fluidic chip substrate has capillary to sample to be tested
The driving layer of active force obtains the no benzene micro-fluidic chip based on 3 D-printing.
2. the no benzene facture of microchip method according to claim 1 based on 3 D-printing, which is characterized in that
Being laid with the method for driving layer includes：
Method one：The slurry of powder with capillary force is poured into flow path groove, is dried；The slurry be paper pulp or
By in starch, cellulose, chitin, hemicellulose one or more powders and water be mixed to form；
Method two：Place mat has the flexible paper of capillary force in flow path groove, obtains driving without pump based on 3 D-printing
Dynamic micro-fluidic chip.
3. the no benzene facture of microchip method according to claim 1 or 2 based on 3 D-printing, feature exist
In in step (2), the filling rate during 3 D-printing is 50~80.
4. the no benzene facture of microchip method according to claim 1 or 2 based on 3 D-printing, feature exist
In in step (2), the material that 3 D-printing uses is polylactic acid or acrylonitrile-butadiene-styrene (ABS) polymer.
5. the no benzene facture of microchip method according to claim 1 or 2 based on 3 D-printing, feature exist
In the mass ratio of dimethyl silicone polymer and curing agent is (8-15):1.
6. the no benzene facture of microchip method according to claim 2 based on 3 D-printing, which is characterized in that
The mass ratio of powder and water is 1 in slurry:(3-8), slurry fill entire runner when pouring into runner.
7. the no benzene facture of microchip method according to claim 2 based on 3 D-printing, which is characterized in that
In step (4), drying temperature is 50~70 degrees Celsius, and drying time is 40~120 minutes.
8. the no benzene facture of microchip method according to claim 1 or 2 based on 3 D-printing, feature exist
In in step (3), drying temperature is 50~70 degrees Celsius, and drying time is 40~120 minutes.
9. the no benzene facture of microchip method according to claim 2 based on 3 D-printing, which is characterized in that
The diameter of powder is 74-125 μm in slurry.
10. a kind of micro-fluidic chip, which is characterized in that it described in claim 1~9 any claim based on three-dimensional by being beaten
The no benzene facture of microchip method of print makes to obtain.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610061308.6A CN105642377B (en) | 2016-01-28 | 2016-01-28 | No benzene facture of microchip method based on 3 D-printing and product |
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| CN106732840A (en) * | 2017-01-24 | 2017-05-31 | 厦门大学 | The 3D printing method and device of nanofiber paper substrate layered manufacturing micro-fluidic chip |
| CN106994369A (en) * | 2017-05-22 | 2017-08-01 | 东南大学 | Regulatable micro-fluidic integrated device of flux and preparation method thereof |
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